Spg008569 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008569
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	biotin--protein ligase 2-like
Location	scaffold10: 34563787 .. 34569843 (-)
RNA-Seq Expression	Spg008569
Synteny	Spg008569

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAATTTCTTCAAAAAAAAAAAAAAAAAAAGTATTAGGTATACTAATTCACGTAGTTGTCCAATTTTATAAGAGAAATACCAACCTGCCTCATTTGGCAAATCCAAAAGAATTTTAGAACTCTCAGCCGGCTCAGAAATCTCGGCGGTCTCAGCCAAGTTCATGATTCAAAATCACACTAGAATCAAGATCAAAGAACGGTCATCCCACCAGCCATCTTATTCTTCGTCATCTATGGCTTCCAATGCATCTGTGATGCCAATTTATCAACGCGTTTCGTGACTGCAAAGCCCATGTCTTGTTTTTTCCGCACCAGCTCGCAGAATCTTGCAAGGAGGCTCTTCGTAAGAGAAACAAGATGCTCATGACATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAGATTCTCCCTCTCTGAGGCCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGGTTTGTTTCTTGCTTGTTTCAATAAATGCTGGAGTAATTTTCCTGAAAATCTGGGTTCATTACGTTGATTTGGGACGAATTTTACTCTACGGAAGTTCGGTCGAACTGTTTCATAAAGATTTATGCGTTTTTCTGAAAAGAAGCTGAATAACCATATGGGGTATTGGTATTCTTCTGGAAAACAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGTTCTCTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGTGGCTGAGAATGAAACTGCCAAATTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGGTACTGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGAATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCAGTAAGTGTATCGAAGCATTTTTTTTCCTCACCATTTTTTCGTTCGTTGACGAGCTAGCTAACTATTAGTTTGCAACAGCAACTTTAGCGACTTTCCATTGGGTGCTGTCTGTGTGGCTGATGTACAGTTTAAGGGAAGAGGTGTGTAATGCCAATAAGTAACGACAAACTGGTAACATTGTTTGTTAGTTCTGGAATTCCTCTTTCTATTTAAAGTCACTTTGTGAATTTAAGTGAAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTTATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGTAACTTTTGTCATGTTTTGGCCCATATCAGCAACCCTTATGGAATCTTTGTTGTCATTTGGTAACTCGATTCTGGATCCCGTGATATGCTAAATAACCAAAACTTTACGGGTAGGGGATTAAATGAAACTGCAAGAGAAAAACTTATGGTCATGGAAGGAGGAAACAGTTTGTCAGAAAGAATCTGGGGGGACTTGGGAGTATATAGGATTTCAAGTAGTGGTCGACTGGCTTCCTATAACCAGAACTGTAAACGTACACTATGGAGATGTTTATACTCCATTACATATTTCGTTATTTCTACCGTGCCAATGACACGTAGCTATCACACTCATGTTGTGACTATTATTTGTACGCTTTTGAGGTTAATACCGCTCATTCTCTTTGTGTTTCTATTTCAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTAATTGTGTTTGTTTCCTTTTCCATCTATTTTTCTTCTACAGTCCTTTTTCCCCTTCCAAATTGAAACTTATAGTCTTATTGCACCAATTTGAAAAGCCTAGTGTCGCTGTGATATATTTTAACTGCTGTGTTCTTCCAGGTATAGGTTTGAATGTAGACAACGAAAAACCAACAACATGCTTGAATGAAGCTCTTACAAATTTATCCAGTACACCTTACAAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAACCTGTACGATATTTTTATAAATCAAGGTATTTATATGTCAATCTAACGTCTTCCTCATGATAAGACATTGTCACATTCATGCATAGATTTAGAGCTTCTCCTTCAATCCAACCTTAACAGTGCTCTGGAGATGCTTGTGAGTTATGAAAATTCTAAGCAGCTATCATCATTTGCGAAAAGAATTTGAAATTGTCCATCTCCTCTTCTTTTTCCTCATCACTGAGCATGGTAGTGTGAACATCATTCTATCACTTCATGCTCTTCGTTGCCTAAGGTTTTGTGCATGCTAAGCATTTGGCAAAGGATAGGACTTCAGATGAATAATTATACGTAAAGGAAATCTTCCTAAGTTTTCTAGTTACTCATCCATCAAGTCTGTAAAGATGATAGCTATATGTAATATTTCCTCTGAAGGGGGTGAGAGAGAGAGCTAAGATATTGTTCGTTTAGGATGTAGATTTTTAATCTTATTCTCTTGTGAAAAAGCTAGAATTTTTGTATCACACGTATTTCACTTATTGTTACCTTCCTAACATGCTGTCATACCTCCAAATATAGTCCACATCATGTTTATCTCAAGTTTGTTATGCTTCAATTTCAGAATTTCCCAGGTGACCTCATTCTGACTAAATCCGTGAATAATGGCATTTTGTTTTCATCATTTCTGAATAATTAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGGTAACTAATGCTGTGGATTAATTTATCTTCTTTATATATATATATATTCAACACATATTGTGTTCCAAGATTCTCACGATTATACTTAGTTTGCATTGTCTATACATTTGTTGTCATGGAAACCAAAAACATCGTGGAAATGGAAAATGTTGTTTGATTCTTTAAGTGCTTAATGTCTATGGAACTAGTAATACTTTGCTTATGTTGCCGTGGGACTCTGTCTTTGCACAAGTCAAACGTCACTTGTTTCAATTGTTTTGTTGACAATTTATGACATATAATATTGTGCAAGTTATGCTAAGTAGGTCTTACACTTTCTTCCCTATAGATATGACCTAAGAAGCATGGACACTTTAGTTTGACTAGCGTATCCGTGTCCGACACTTGTTGGACACGTATCGGACACTTATTAGCACAATTGATATGTAGCAAACACCAGTTGTAGGTTCACTTCGAATCCTTGTTAAGTATACTAAATAGACGCAATACTTAAATGACAAAAATAAAAAAATTTAGGAGTACAATACATTAAACTTATTTTTTTCAAACATACATTGCATGAACTTATTGATTTTGAATTTCCTTTTTGTATAAAAACAATATATATTTTAAAAAATGTATATTTTCTAAACGTGTCCGTGTCCTAGATTTTAAAAAAATGACGTGTTGCCATGTCTGTGTCGTGTCGTATCCGTGTCTCGTGTCCGTATCCGTGCTTCTTAGGATATGACTTTGTTTGGATCTATCTTGTAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGTACGTGATATCTGAATGATAGCTGATCCTGTGGCAGCTCTAGTATTTACACAAATGCTGCAAAGTGCAAACTTAATCCCACTACTCTTTTTGGTTCTTCACTGTGGATATTTATATATTTTTGGCTCAGAAGAAAAGACCTTGTCTCCTGCAGTTGAATTTGTTTTTATCTTGTAGTTAGATAATCCTATTCCTGAAGGATGTTAAATATTCGACATCAATTTGTTGTTCAACTTAGTACTGGAGAATGCTGAATGCACTACATTCTATGATTTTCATGGTTTTAAACACTAAATTCGTTGCTTATTGGTCAGAAATTCTTGTTTTCTTATATAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGGTCAAGTATTTGAATGGATTCCTATTCTTGATTTAGTGAATTAACTATGCTGTGCTGATATTTTTGCAAAGTTTGACACATACAGTTTGATACATCGACAGTGAGTGTACGACATCTGTGTCTATAGCATATCAATTGCATTTTGAAACTAATCCTGAAAGATTGAGAGAGTTCATTGTACATTAAGAAAAGTGACTTTGATATATGTATACCATGTGCCCTAACCTGAACGTTCAAACAACAGATAGGATAACTTGATAAGTCATCTGCTTCAGGCTTTCACCGAAAATCTGGTCACAACATTCTAAAATTTTTTACTTCCTGTTTATACTGTAGATCTGCTTACGAAAAGGAAAAGGTCTATCATAACTAAACTAAATCATTATTATTTACAAAATTGACTGTGAAATTGATTCCAAGTTCATGCATGACTAAGAAACAGTGGTCAATAAAGTTAACGTCTTCAAAGTGCTTTAGAATCAATCATAATGATTATAAAGGAAGTGACTGCCCTGTTGGCCAGTCAACCAATATTGTAGTTTCTGCTGTACTCAGTTGGATGAATTTTTAATTCTGATTTATTTATTGCTCAAAAGTCCTTTAGAACTAAGCTGGCAGCAATATCTACATGCCCTGTTCTGCATTGCATGCATCAGCTCACAACCCCAAGCATTTGAAAATTTTAACCATCTGAATTCTTCTCCAAATGCAATTGACTGTAGAGATCTGATTTTAGAAGGCTAATATGTGTAATAGATCTGATCTTAAATCTGATGATCATGTCATCTTCCTATTGTTCTGCAGTTTGGACTTTTTCAAAGGACTGATCAAGAGAAAACTGGAGTGAGAATGCTGCTTTTTCACTTTCCAACAGGTGGCTGACTATGAGCTGTATGGAATGGAATCATGTGCAATAAGAAGGAGACATTTGCTCTCTTTTATTTTTGGCAAACAATTCTCTGTTTCTAAACTGGAGGAAAGAAAGAGAAAGATATCTTACTTTTTATGATTAAACAATGAGATCTCTACTGAAGTTGTAAATATTATTTATCTTTAAACTTACTAAGGAGGGTCCATGGCCCAGAACGTGCTATATTTTTGTATCATCCTATTGAACGTGAAGGAATTCCGAGTGAGACAATTTCCAATTGATTAAAACTGTCCCCACAATTGGCATAATCAGTCAGTCAGCAGTTCTTTTTTGTTAGAAACTTAAGAAATTCTCTGCCACTTTATTGCCAGCTGTTGAGCTTGAAACTGAAACCCCGTTATGACAGAAGGTGTTGCGTTGTAATGGAGAACGGATGGAACTTATTTGAACTTTACAACTGTGAAATACGTGAAAAGAAACAGAAGGGCAAAAGATTCTTGTGGCGAGGCGCCATGTCAGACCCAGAAACTGGTATGTCTTCTTCTATACATTTCCTTACGGTTGTTGGGGCCTTTTGTTTAGGATTCACTATCAGAATAGAGAGAAGGGATTGGCTTGCCACATGCAGAACAGTGGATGGATTGAAAAACCCAAAAGGGTTCTATCGTAACATAAGTTTGGTCTCTGAACTTTCAATATCGTTTTTGTACCTAATAGTTTTCGAGACGTTACATGCTAACTATACTATTAAAGTTAGAGTTAGGTGGTGGGCTGATGTCAGGCTGGCTGAGGTGTTTGGTAGTGTTAGCATTGAAATGACGTAGTTGACGACATGAACTTATTAGACACAGACTTAATGGAAACTCGTGAAACTTTGGGACAATGCTCGTAATTCAAGTCAGCTTTGTATTGGTTTTGGAGGGGGTGGTGGGGGTCATGCAAGGACAGAGTTGGGCGGTGGGAGTGTGGAGTTTGAGGTAAACAAAAGCTGCAAAAGTGAGAAAGCCAGAAGCTGTGAAACTGGGTTTGTGTTCAGCTTATGTTATGATGTGCCCCTCAAGTAG

mRNA sequence

ATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAGATTCTCCCTCTCTGAGGCCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGTTCTCTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGTGGCTGAGAATGAAACTGCCAAATTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGGTACTGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGAATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCATCACTTTGTGAATTTAAGTGAAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTTATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTATAGGTTTGAATGTAGACAACGAAAAACCAACAACATGCTTGAATGAAGCTCTTACAAATTTATCCAGTACACCTTACAAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAACCTGTACGATATTTTTATAAATCAAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGGTCAAATCTGCTTACGAAAAGGAAAAGAAGGTGTTGCGTTGTAATGGAGAACGGATGGAACTTATTTGAACTTTACAACTGTGAAATACGTGAAAAGAAACAGAAGGGCAAAAGATTCTTGTGGCGAGGCGCCATGTCAGACCCAGAAACTGTTTTCGAGACGTTACATGCTAACTATACTATTAAAGTTAGAGTTAGGTGGTGGGCTGATGTCAGGCTGGCTGAGCTTTGTATTGGTTTTGGAGGGGGTGGTGGGGGTCATGCAAGGACAGAGTTGGGCGGTGGGAGTGTGGAGTTTGAGGTAAACAAAAGCTGCAAAAGTGAGAAAGCCAGAAGCTGTGAAACTGGGTTTGTGTTCAGCTTATGTTATGATGTGCCCCTCAAGTAG

Coding sequence (CDS)

ATGATTTTAACTAACCCACTTCGCTATTTCAGCAGGCGAGACTCGCGCTACATTTTTTCCATCATAGATTCTCCCTCTCTGAGGCCTCTCAGCAAAACAACTGCTTCAGCTCCCACCCCAGAAACTTCCATTAATGTGAGGGAAAAAAGGAGATTGTTCTCTTCAGCAATGGAGACTAGTTCAACATGTGCGTTGGTTTTGAGTGGAAAAACAGTGGCTGAGAATGAAACTGCCAAATTGCTGAAGAGAAATGACACTTTGAAGCTCCATGACGGTACTGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTATGGAGGAAAGTGGTTTTCAGATTGATTTCTATATGAATGCTCTTTCTACTAATACTTATGGTAGATTCCTCATTTGGTGTCCACGGATTCCTTCAACTCATGATGTCATTTCTCATCACTTTGTGAATTTAAGTGAAGGTCGATCAAAGAATTTGTGGGAATCACCCCCTGGTTGCCTTATGTTTTCCTTTACCATTCAAATGGAAGATGGGCGTATTGTTCCTCTGCTGCAGTATGTAATATCTCTCGCTATTACCGAGGCCATAAAAGATATTTGCGACAAAAAGGGTTTACCTTATATTGATTTGAAGATAAAGTGGCCAAACGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTATGCACTTCAACATATAGATCAAAGAAGTTCAACGTTAGTGCTGGTATAGGTTTGAATGTAGACAACGAAAAACCAACAACATGCTTGAATGAAGCTCTTACAAATTTATCCAGTACACCTTACAAGCTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAAACCTGTACGATATTTTTATAAATCAAGGGTTTCAGGCTCTTCAGGAACTCTACTGTCGGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAAGAAAAGAAAGGAGACCAAGTAGTGGAAAATGTAGTCACTATTCAGGGGTTGACATCTTCAGGATATTTGCTAGCTATTGGAGATGACCACCAAATGTGCGAGCTTCATCCCGATGGAAATAGGTCAAATCTGCTTACGAAAAGGAAAAGAAGGTGTTGCGTTGTAATGGAGAACGGATGGAACTTATTTGAACTTTACAACTGTGAAATACGTGAAAAGAAACAGAAGGGCAAAAGATTCTTGTGGCGAGGCGCCATGTCAGACCCAGAAACTGTTTTCGAGACGTTACATGCTAACTATACTATTAAAGTTAGAGTTAGGTGGTGGGCTGATGTCAGGCTGGCTGAGCTTTGTATTGGTTTTGGAGGGGGTGGTGGGGGTCATGCAAGGACAGAGTTGGGCGGTGGGAGTGTGGAGTTTGAGGTAAACAAAAGCTGCAAAAGTGAGAAAGCCAGAAGCTGTGAAACTGGGTTTGTGTTCAGCTTATGTTATGATGTGCCCCTCAAGTAG

Protein sequence

MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFSSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNLSEGRSKNLWESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNRSNLLTKRKRRCCVVMENGWNLFELYNCEIREKKQKGKRFLWRGAMSDPETVFETLHANYTIKVRVRWWADVRLAELCIGFGGGGGGHARTELGGGSVEFEVNKSCKSEKARSCETGFVFSLCYDVPLK

Homology

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BLAST of Spg008569 vs. NCBI nr
Match: XP_023517017.1 (biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.3 bits (1531), Expect = 9.2e-166
Identity = 304/374 (81.28%), Postives = 322/374 (86.10%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M LTNPLR FS R SR   SII+SPS R    TTASAPTP TS  V EK RLFS     A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL D  EIS+SLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISISLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS +F +L               GRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYV+SLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVVSLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 358
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

[Close]

BLAST of Spg008569 vs. NCBI nr
Match: XP_022990857.1 (biotin--protein ligase 2-like [Cucurbita maxima])

HSP 1 Score: 594.0 bits (1530), Expect = 1.2e-165
Identity = 306/374 (81.82%), Postives = 320/374 (85.56%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M LTNPLR FS R SR   SII SPS R    TTASAPTP TS  V EK RLFS     A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           ME SSTCALVLSGKT AENETAKLLKRNDTLKL D  EISVSLHSERDKS++ESGFQI  
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS +F +L               GRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 358
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

[Close]

BLAST of Spg008569 vs. NCBI nr
Match: XP_022957207.1 (biotin--protein ligase 2-like [Cucurbita moschata])

HSP 1 Score: 593.6 bits (1529), Expect = 1.6e-165
Identity = 305/374 (81.55%), Postives = 322/374 (86.10%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M LTNPLR FS R SR   SII+SPS R    TTASAPTP TS  V EK RLFS     A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL D  EISVSLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS +F +L               GRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDK+GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 358
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

[Close]

BLAST of Spg008569 vs. NCBI nr
Match: XP_016900373.1 (PREDICTED: biotin--protein ligase 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 587.0 bits (1512), Expect = 1.5e-163
Identity = 291/379 (76.78%), Postives = 326/379 (86.02%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFS    S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL D TEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISH+F NL               GRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNRSNLL 363
           GDD+QMCELHPDGN  ++L
Sbjct: 361 GDDNQMCELHPDGNSQSML 379

[Close]

BLAST of Spg008569 vs. NCBI nr
Match: XP_016900374.1 (PREDICTED: biotin--protein ligase 2-like isoform X6 [Cucumis melo])

HSP 1 Score: 586.3 bits (1510), Expect = 2.5e-163
Identity = 291/375 (77.60%), Postives = 324/375 (86.40%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFS    S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL D TEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISH+F NL               GRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 359
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

[Close]

BLAST of Spg008569 vs. ExPASy Swiss-Prot
Match: Q9SL92 (Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 PE=1 SV=2)

HSP 1 Score: 420.2 bits (1079), Expect = 3.1e-116
Identity = 201/330 (60.91%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 54  SSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSM--EESG 113
           +SAME+ ++C+LVL GK+  E E AK LK  ++LKL D T++S+ L SE    +  +++ 
Sbjct: 36  ASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNS 95

Query: 114 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNL 173
           F +  +MN++ T+ +GRFLIW PR+ STHDV+SH+F  L               GR+KN+
Sbjct: 96  FNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNV 155

Query: 174 WESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLY 233
           WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLPYID+KIKWPNDLY
Sbjct: 156 WESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLY 215

Query: 234 VNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILA 293
           VNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++     L+RE+IL 
Sbjct: 216 VNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILG 275

Query: 294 FFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSG 353
            FF+KFE  +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV+NVVTIQGLTSSG
Sbjct: 276 AFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSG 335

Query: 354 YLLAIGDDHQMCELHPDGNRSNLLTKRKRR 369
           YLLA+GDD+QM ELHPDGN  +      RR
Sbjct: 336 YLLAVGDDNQMYELHPDGNSFDFFKGLVRR 365

[Close]

BLAST of Spg008569 vs. ExPASy Swiss-Prot
Match: F4I4W2 (Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.1e-112
Identity = 194/327 (59.33%), Postives = 249/327 (76.15%), Query Frame = 0


Query: 57  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSM--EESGFQI 116
           M+  ++C+LVL GK+  E +TA  LK N+ LKL D +++S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 117 DFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWES 176
             +MN++ST+ +GRFLIW P + STHDV+SH+F  +               GR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 177 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 236
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 237 LKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFF 296
           LK+GGILCTSTYRS+KF VS G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 297 NKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLL 356
            KFEN +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 357 AIGDDHQMCELHPDGNRSNLLTKRKRR 369
           AIGDD+ M ELHPDGN  +      RR
Sbjct: 301 AIGDDNVMYELHPDGNSFDFFKGLVRR 327

[Close]

BLAST of Spg008569 vs. ExPASy Swiss-Prot
Match: P50747 (Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.4e-31
Identity = 81/275 (29.45%), Postives = 142/275 (51.64%), Query Frame = 0


Query: 112 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL---------------SEGRSK 171
           F ++ Y   L T   G+ +++    P+T  ++                       +GR  
Sbjct: 451 FNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGG 510

Query: 172 NLWESPPGC----LMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIK 231
           N+W SP GC    L+ S  ++ + G+ +P +Q+++S+A+ EA++ I + +    I+L++K
Sbjct: 511 NVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQD---INLRVK 570

Query: 232 WPNDLYVNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLS----ST 291
           WPND+Y + L K+GG+L  ST   + F +  G G NV N  PT C+N+ +T  +    + 
Sbjct: 571 WPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAE 630

Query: 292 PYKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVEN 351
              LR + ++A      E L   F ++G  ++  LY R W+HSGQ+V +   +G +    
Sbjct: 631 LKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPK---- 690

Query: 352 VVTIQGLTSSGYLLAIGDDHQMCELHPDGNRSNLL 363
            V+I GL  SG+L    +  ++  +HPDGN  ++L
Sbjct: 691 -VSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDML 717

[Close]

BLAST of Spg008569 vs. ExPASy Swiss-Prot
Match: Q920N2 (Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.0e-30
Identity = 81/275 (29.45%), Postives = 135/275 (49.09%), Query Frame = 0


Query: 112 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL---------------SEGRSK 171
           F ++ Y   L T   G+ +++     +T  ++      +                +GR  
Sbjct: 447 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGP 506

Query: 172 NLWESPPGC----LMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIK 231
           N W SP GC    L+    ++ + G+ +P +Q+++SLA+ EA++ I    G   I+L++K
Sbjct: 507 NAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSI---PGYEDINLRVK 566

Query: 232 WPNDLYVNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTP--- 291
           WPND+Y + L K+GG+L  ST   + F +  G G NV N  PT C+N+ +   +      
Sbjct: 567 WPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLIEEHNKQHGAG 626

Query: 292 -YKLRREDILAFFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVEN 351
              LR + ++A      E L D F +QG   +  LY + W+H GQ+V +   +G Q    
Sbjct: 627 LKPLRADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWVHGGQQVRLGSTEGPQ---- 686

Query: 352 VVTIQGLTSSGYLLAIGDDHQMCELHPDGNRSNLL 363
             +I GL  SG+L    +D  +  +HPDGN  ++L
Sbjct: 687 -ASIVGLDDSGFLQVHQEDGGVVTVHPDGNSFDML 713

[Close]

BLAST of Spg008569 vs. ExPASy Swiss-Prot
Match: O14353 (Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bpl1 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.1e-24
Identity = 91/323 (28.17%), Postives = 150/323 (46.44%), Query Frame = 0


Query: 84  NDTLKLHDGTEISVSLHSERDKSMEESGFQIDFYMNALSTNTYGRFLIWCPRIPSTHDVI 143
           N+ +K+  G E       E+     +  F ++ Y   ++   +G  +I  P I ST  ++
Sbjct: 317 NEIVKIWYGDE-------EKICKKAKPSFDLELYAKLINGCRFGLPIIVAPVIRSTQTLL 376

Query: 144 SHHFVNL----------------SEGRSKNLWESPPGCLMFSFTIQMEDGRI----VPLL 203
             ++  L                  GR +N+W SP G L FSF I ++        + L 
Sbjct: 377 DKNYRFLDSTNTGFTVLGNYQTAGRGRGQNMWVSPYGTLAFSFIINVDAKNFSTTPIALF 436

Query: 204 QYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYV------------NGLKVGGILCTS 263
           QY+++LA+   I++     G   I   IKWPND+YV              +K+ GI+ TS
Sbjct: 437 QYLMALAVVRGIREYA--PGYENIPAFIKWPNDVYVRVDKGGINFQGKQYMKLSGIIVTS 496

Query: 264 TYRSKKFNVSAGIGLNVDNEKPTTCLNEALT--NLSSTPYKLRR---EDILAFFFNKFEN 323
            YR    ++  G G+NV N  PT  LN  +   N +S   +L +   E +LA   N+F+ 
Sbjct: 497 NYRKNVLHLVVGCGINVSNLGPTVSLNTLVDEWNKNSDNPRLEKFSFEKLLASVLNQFDR 556

Query: 324 LYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSS-GYLLA--I 363
            + + + +GF  +   Y + WLHS Q V +    G + +     IQG+TS  G+LLA  +
Sbjct: 557 YHRLLLEEGFSLILPEYYQYWLHSNQTVNL--ASGGKAI-----IQGITSDFGFLLAQLL 616

[Close]

BLAST of Spg008569 vs. ExPASy TrEMBL
Match: A0A6J1JK17 (biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 5.8e-166
Identity = 306/374 (81.82%), Postives = 320/374 (85.56%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M LTNPLR FS R SR   SII SPS R    TTASAPTP TS  V EK RLFS     A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           ME SSTCALVLSGKT AENETAKLLKRNDTLKL D  EISVSLHSERDKS++ESGFQI  
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS +F +L               GRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDKKGLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 358
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

[Close]

BLAST of Spg008569 vs. ExPASy TrEMBL
Match: A0A6J1GYI6 (biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 7.6e-166
Identity = 305/374 (81.55%), Postives = 322/374 (86.10%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M LTNPLR FS R SR   SII+SPS R    TTASAPTP TS  V EK RLFS     A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           METSSTC+LVLSGKT AENETAKLLKRNDTLKL D  EISVSLHSERDKS++ESGFQI  
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           YMN+LST+T+GRFLIWC RIPST DVIS +F +L               GRSKNLWESPP
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGRIVPLLQYVISLAITEA+KDICDK+GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGGILCTSTYRSKKFNV+AGIGLNVDN+KPTTCLNEAL NLSSTPYK RREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 358
           FE LYDIFINQGFQAL+ELYC+TWLHSGQRVIVQEKK DQVVENVVTIQGLT +GYLLAI
Sbjct: 301 FEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENVVTIQGLTPTGYLLAI 360

[Close]

BLAST of Spg008569 vs. ExPASy TrEMBL
Match: A0A1S4DWM1 (biotin--protein ligase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 7.1e-164
Identity = 291/379 (76.78%), Postives = 326/379 (86.02%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFS    S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL D TEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISH+F NL               GRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 360
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

Query: 361 GDDHQMCELHPDGNRSNLL 363
           GDD+QMCELHPDGN  ++L
Sbjct: 361 GDDNQMCELHPDGNSQSML 379

[Close]

BLAST of Spg008569 vs. ExPASy TrEMBL
Match: A0A1S4DWL1 (biotin--protein ligase 2-like isoform X6 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 1.2e-163
Identity = 291/375 (77.60%), Postives = 324/375 (86.40%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFS    S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL D TEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISH+F NL               GRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 359
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

[Close]

BLAST of Spg008569 vs. ExPASy TrEMBL
Match: A0A5A7U4Q8 (Biotin--protein ligase 2-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G00810 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 4.6e-163
Identity = 290/374 (77.54%), Postives = 323/374 (86.36%), Query Frame = 0


Query: 1   MILTNPLRYFSRRDSRYIFSIIDSPSLRPLSKTTASAPTPETSINVREKRRLFS----SA 60
           M+L N LRYF RRDSR++FS+++S S   LS+  AS PTP  SIN RE+RRLFS    S 
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSMEESGFQIDF 120
           M+ S TC+LVLSGKT AENETAKLLKRNDTLKL D TEISV LHSE+DK +EE+GF+ID 
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWESPP 180
           Y+NALST+T+GRFLIW PR+PST DVISH+F NL               GRSKNLWESPP
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLK 240
           GCLMFSFTIQMEDGR VPLLQYVISLAITEAIKDICDK+GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNK 300
           VGG+LCTSTYR KKFNV+AGIGLNV+N+KP+TCLNEALT+LSSTPYK R+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

Query: 301 FENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAI 358
           FE LYD+FINQGF+AL+ELY +TWLHSGQRV+VQEKK DQVVENVVTIQGLTSSGYLLAI
Sbjct: 301 FERLYDVFINQGFRALEELYYQTWLHSGQRVVVQEKKEDQVVENVVTIQGLTSSGYLLAI 360

[Close]

BLAST of Spg008569 vs. TAIR 10
Match: AT2G25710.1 (holocarboxylase synthase 1 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.2e-117
Identity = 201/330 (60.91%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 54  SSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSM--EESG 113
           +SAME+ ++C+LVL GK+  E E AK LK  ++LKL D T++S+ L SE    +  +++ 
Sbjct: 36  ASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNS 95

Query: 114 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNL 173
           F +  +MN++ T+ +GRFLIW PR+ STHDV+SH+F  L               GR+KN+
Sbjct: 96  FNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNV 155

Query: 174 WESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLY 233
           WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLPYID+KIKWPNDLY
Sbjct: 156 WESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLY 215

Query: 234 VNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILA 293
           VNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++     L+RE+IL 
Sbjct: 216 VNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILG 275

Query: 294 FFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSG 353
            FF+KFE  +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV+NVVTIQGLTSSG
Sbjct: 276 AFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSG 335

Query: 354 YLLAIGDDHQMCELHPDGNRSNLLTKRKRR 369
           YLLA+GDD+QM ELHPDGN  +      RR
Sbjct: 336 YLLAVGDDNQMYELHPDGNSFDFFKGLVRR 365

[Close]

BLAST of Spg008569 vs. TAIR 10
Match: AT2G25710.2 (holocarboxylase synthase 1 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.2e-117
Identity = 201/330 (60.91%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 54  SSAMETSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSM--EESG 113
           +SAME+ ++C+LVL GK+  E E AK LK  ++LKL D T++S+ L SE    +  +++ 
Sbjct: 36  ASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNS 95

Query: 114 FQIDFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNL 173
           F +  +MN++ T+ +GRFLIW PR+ STHDV+SH+F  L               GR+KN+
Sbjct: 96  FNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNV 155

Query: 174 WESPPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLY 233
           WESP GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGLPYID+KIKWPNDLY
Sbjct: 156 WESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLY 215

Query: 234 VNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILA 293
           VNGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLN  L  ++     L+RE+IL 
Sbjct: 216 VNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILG 275

Query: 294 FFFNKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSG 353
            FF+KFE  +D+F++QGF++L+ELY RTWLHS QRVIV++K  DQVV+NVVTIQGLTSSG
Sbjct: 276 AFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSG 335

Query: 354 YLLAIGDDHQMCELHPDGNRSNLLTKRKRR 369
           YLLA+GDD+QM ELHPDGN  +      RR
Sbjct: 336 YLLAVGDDNQMYELHPDGNSFDFFKGLVRR 365

[Close]

BLAST of Spg008569 vs. TAIR 10
Match: AT1G37150.2 (holocarboxylase synthetase 2 )

HSP 1 Score: 407.5 bits (1046), Expect = 1.5e-113
Identity = 194/327 (59.33%), Postives = 249/327 (76.15%), Query Frame = 0


Query: 57  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSM--EESGFQI 116
           M+  ++C+LVL GK+  E +TA  LK N+ LKL D +++S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 117 DFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWES 176
             +MN++ST+ +GRFLIW P + STHDV+SH+F  +               GR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 177 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 236
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 237 LKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFF 296
           LK+GGILCTSTYRS+KF VS G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 297 NKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLL 356
            KFEN +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

Query: 357 AIGDDHQMCELHPDGNRSNLLTKRKRR 369
           AIGDD+ M ELHPDGN  +      RR
Sbjct: 301 AIGDDNVMYELHPDGNSFDFFKGLVRR 327

[Close]

BLAST of Spg008569 vs. TAIR 10
Match: AT1G37150.3 (holocarboxylase synthetase 2 )

HSP 1 Score: 361.7 bits (927), Expect = 9.3e-100
Identity = 170/291 (58.42%), Postives = 223/291 (76.63%), Query Frame = 0


Query: 57  METSSTCALVLSGKTVAENETAKLLKRNDTLKLHDGTEISVSLHSERDKSM--EESGFQI 116
           M+  ++C+LVL GK+  E +TA  LK N+ LKL D +++S+ L SE    +  ++S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 117 DFYMNALSTNTYGRFLIWCPRIPSTHDVISHHFVNL-------------SEGRSKNLWES 176
             +MN++ST+ +GRFLIW P + STHDV+SH+F  +               GR+KN+WES
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 177 PPGCLMFSFTIQMEDGRIVPLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNG 236
           P GCLM+SFT++MEDGR+VPL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 237 LKVGGILCTSTYRSKKFNVSAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFF 296
           LK+GGILCTSTYRS+KF VS G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 297 NKFENLYDIFINQGFQALQELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQ 333
            KFEN +D+F+ QGF++L+ELY RTWLHSGQRVI +EK  DQVV+NVVTIQ
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQ 291

[Close]

BLAST of Spg008569 vs. TAIR 10
Match: AT1G37150.4 (holocarboxylase synthetase 2 )

HSP 1 Score: 345.1 bits (884), Expect = 9.0e-95
Identity = 161/248 (64.92%), Postives = 196/248 (79.03%), Query Frame = 0


Query: 134 PRIPSTHDVISHHFVNL-------------SEGRSKNLWESPPGCLMFSFTIQMEDGRIV 193
           P + STHDV+SH+F  +               GR+KN+WESP GCLM+SFT++MEDGR+V
Sbjct: 37  PYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMYSFTLEMEDGRVV 96

Query: 194 PLLQYVISLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNV 253
           PL+QYV+SLA+TEA+KD+CDKKGL Y D+KIKWPNDLY+NGLK+GGILCTSTYRS+KF V
Sbjct: 97  PLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGILCTSTYRSRKFLV 156

Query: 254 SAGIGLNVDNEKPTTCLNEALTNLSSTPYKLRREDILAFFFNKFENLYDIFINQGFQALQ 313
           S G+GLNVDNE+PTTCLN  L ++      L+RE+IL  FF KFEN +D+F+ QGF++L+
Sbjct: 157 SVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFFDLFMEQGFKSLE 216

Query: 314 ELYCRTWLHSGQRVIVQEKKGDQVVENVVTIQGLTSSGYLLAIGDDHQMCELHPDGNRSN 369
           ELY RTWLHSGQRVI +EK  DQVV+NVVTIQGLTSSGYLLAIGDD+ M ELHPDGN  +
Sbjct: 217 ELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLLAIGDDNVMYELHPDGNSFD 276

The following BLAST results are available for this feature:

BLAST of Spg008569 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023517017.1	9.2e-166	81.28	biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022990857.1	1.2e-165	81.82	biotin--protein ligase 2-like [Cucurbita maxima]	[more]
XP_022957207.1	1.6e-165	81.55	biotin--protein ligase 2-like [Cucurbita moschata]	[more]
XP_016900373.1	1.5e-163	76.78	PREDICTED: biotin--protein ligase 2-like isoform X2 [Cucumis melo]	[more]
XP_016900374.1	2.5e-163	77.60	PREDICTED: biotin--protein ligase 2-like isoform X6 [Cucumis melo]	[more]

BLAST of Spg008569 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SL92	3.1e-116	60.91	Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 ...	[more]
F4I4W2	2.1e-112	59.33	Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1	[more]
P50747	1.4e-31	29.45	Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1	[more]
Q920N2	2.0e-30	29.45	Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1	[more]
O14353	2.1e-24	28.17	Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX...	[more]

BLAST of Spg008569 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JK17	5.8e-166	81.82	biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 S...	[more]
A0A6J1GYI6	7.6e-166	81.55	biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3...	[more]
A0A1S4DWM1	7.1e-164	76.78	biotin--protein ligase 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489963...	[more]
A0A1S4DWL1	1.2e-163	77.60	biotin--protein ligase 2-like isoform X6 OS=Cucumis melo OX=3656 GN=LOC103489963...	[more]
A0A5A7U4Q8	4.6e-163	77.54	Biotin--protein ligase 2-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of Spg008569 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25710.1	2.2e-117	60.91	holocarboxylase synthase 1	[more]
AT2G25710.2	2.2e-117	60.91	holocarboxylase synthase 1	[more]
AT1G37150.2	1.5e-113	59.33	holocarboxylase synthetase 2	[more]
AT1G37150.3	9.3e-100	58.42	holocarboxylase synthetase 2	[more]
AT1G37150.4	9.0e-95	64.92	holocarboxylase synthetase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004408	Biotin--acetyl-CoA-carboxylase ligase	TIGRFAM	TIGR00121	TIGR00121	coord: 152..342 e-value: 6.0E-28 score: 96.0
IPR004408	Biotin--acetyl-CoA-carboxylase ligase	CDD	cd16442	BPL	coord: 129..291 e-value: 5.70271E-40 score: 140.091
IPR003142	Biotin protein ligase, C-terminal	PFAM	PF02237	BPL_C	coord: 309..357 e-value: 2.5E-6 score: 27.3
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PFAM	PF03099	BPL_LplA_LipB	coord: 151..247 e-value: 8.8E-25 score: 87.1
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PROSITE	PS51733	BPL_LPL_CATALYTIC	coord: 115..292 score: 24.214273
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 102..306 e-value: 9.6E-34 score: 118.4
None	No IPR available	PANTHER	PTHR12835	BIOTIN PROTEIN LIGASE	coord: 57..362
None	No IPR available	PANTHER	PTHR12835:SF5	HOLOCARBOXYLASE SYNTHETASE (BIOTIN-(PROPRIONYL-COA-CARBOXYLASE (ATP-HYDROLYSING)) LIGASE)	coord: 57..362
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 115..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008569.1	Spg008569.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
molecular_function	GO:0004077	biotin-[acetyl-CoA-carboxylase] ligase activity