Spg008550 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg008550 vs. NCBI nr
Match: XP_038884256.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 520.0 bits (1338), Expect = 1.7e-143
Identity = 281/386 (72.80%), Postives = 320/386 (82.90%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFS--QPVLIPDFADGMQQIRNKSQKSFNVND 60
           MW KFR S+IRN TKL  SI+ C  +LCNFS  QPV I  FAD  Q+I  K QKSFNVN+
Sbjct: 1   MWTKFRHSEIRNSTKLSQSIIACFAHLCNFSQTQPVFISIFADEEQEIGKKPQKSFNVNE 60

Query: 61  EYLCTLRTKSYIEFFTKVQSIVHESPPSTSSSS--TDLRRKFSETILLQPGQLEAIPSIL 120
           EYLCTLRTKS+ EFFTK + IV ESPPS +SSS   D  RK SETILLQP +LEA+PSIL
Sbjct: 61  EYLCTLRTKSFSEFFTKAEFIVDESPPSITSSSAAADRCRKLSETILLQPVRLEAVPSIL 120

Query: 121 ESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAE 180
           ES  LLMLPELK L IDY NVSA+ASNLC+RLL N+KS RS SR IQQSLDSIEKC S+E
Sbjct: 121 ESSFLLMLPELKGLFIDYLNVSAKASNLCTRLLTNVKSTRSKSRCIQQSLDSIEKCFSSE 180

Query: 181 KIETIASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTC 240
            IE+IASDLL+ RAPFSD+DKRDF LIH +YTA+S RLNC+RKKV RKIR+IKIID  TC
Sbjct: 181 TIESIASDLLSLRAPFSDLDKRDFTLIHADYTAISRRLNCTRKKVERKIRTIKIIDGITC 240

Query: 241 GLIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAK 300
           GL +IT+RTLT  VMA    PGP  FG+RLKS  RKL+RH+MLRNGGLE+VGE++E AAK
Sbjct: 241 GLNSITTRTLTDSVMA--KDPGPVIFGYRLKSLGRKLVRHEMLRNGGLEKVGEKVEAAAK 300

Query: 301 GSYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQ 360
           GSYI+ RELDTTSRLVVRLGDAVDHGKAM+RLF  RKEDKFAV+VAMDE+KK+N+S+RKQ
Sbjct: 301 GSYIMKRELDTTSRLVVRLGDAVDHGKAMLRLFGGRKEDKFAVSVAMDEVKKNNVSMRKQ 360

Query: 361 VEDVEEHLYMCIVTINRTRTLVINQM 383
           VEDVEEHLY+CIVTINR+R  VI Q+
Sbjct: 361 VEDVEEHLYLCIVTINRSRASVIKQL 384

BLAST of Spg008550 vs. NCBI nr
Match: KAG6602186.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 516.2 bits (1328), Expect = 2.5e-142
Identity = 285/382 (74.61%), Postives = 310/382 (81.15%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDEY 60
           MW KFRAS+                        + I  FAD   QIRNK QKSFNVN+EY
Sbjct: 1   MWTKFRASRF-----------------------IWISVFADVKHQIRNKPQKSFNVNEEY 60

Query: 61  LCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILESPL 120
           LC LR++S++EFF K Q IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILESPL
Sbjct: 61  LCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILESPL 120

Query: 121 LLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIET 180
           L+MLPEL+ LLID+FNVSAEASNLC RLLANLK ARSNSRF+Q+ LDSIEKCSS ++IET
Sbjct: 121 LVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIET 180

Query: 181 IASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIA 240
           I SDLLAQRAP SD+DKRDFA IHD+Y AVS  LN +RKKVARKIRSIKIIDRFTCGL+A
Sbjct: 181 ITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVA 240

Query: 241 ITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYI 300
           ITSR LTALVMAA  GP   +  FRLKS  RKLLRHQML NGGLERVGEQLE AAKGSYI
Sbjct: 241 ITSRGLTALVMAADQGPVLRY--FRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+C VTINR+R  VINQM
Sbjct: 361 EEHLYLCFVTINRSRASVINQM 357

BLAST of Spg008550 vs. NCBI nr
Match: XP_022959620.1 (UPF0496 protein 3-like [Cucurbita moschata])

HSP 1 Score: 515.8 bits (1327), Expect = 3.3e-142
Identity = 285/382 (74.61%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDEY 60
           MW KFRAS+                        + I  FAD   QIRNK QKSFNVN+EY
Sbjct: 1   MWTKFRASRF-----------------------IWISVFADVKHQIRNKPQKSFNVNEEY 60

Query: 61  LCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILESPL 120
           LC LR++S++EFF K Q IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILESPL
Sbjct: 61  LCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILESPL 120

Query: 121 LLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIET 180
           L+MLPEL+ LLID+FNVSAEASNLC RLLANLK ARSNSRF+Q+ LDSIEKCSS ++IET
Sbjct: 121 LVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIET 180

Query: 181 IASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIA 240
           I SDLLAQRAP SD+DKRDFA IHD+Y AVS  LN +RKKVARKIRSIKIIDRFTCGL+A
Sbjct: 181 ITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVA 240

Query: 241 ITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYI 300
           ITSR LTALVMAA  GP   +  FRLKS  RKLLRHQML NGGLERVGEQLE AAKGSYI
Sbjct: 241 ITSRGLTALVMAADQGPVLRY--FRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDHGKAMVRLFVERKE+KFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKENKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 357

BLAST of Spg008550 vs. NCBI nr
Match: KAG7032870.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 514.6 bits (1324), Expect = 7.2e-142
Identity = 284/382 (74.35%), Postives = 309/382 (80.89%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDEY 60
           MW KFRAS+                        + I  FAD   QIRNK QKSFNVN+EY
Sbjct: 1   MWTKFRASRF-----------------------IWISVFADVKHQIRNKPQKSFNVNEEY 60

Query: 61  LCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILESPL 120
           LC LR++S++EFF K Q IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILESPL
Sbjct: 61  LCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILESPL 120

Query: 121 LLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIET 180
           L+MLPEL+ LLID+FNVSAEASNLC RLL NLK ARSNSRF+Q+ LDSIEKCSS ++IET
Sbjct: 121 LVMLPELRGLLIDFFNVSAEASNLCHRLLVNLKLARSNSRFVQKLLDSIEKCSSPKEIET 180

Query: 181 IASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIA 240
           I SDLLAQRAP SD+DKRDFA IHD+Y AVS  LN +RKKVARKIRSIKIIDRFTCGL+A
Sbjct: 181 ITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVA 240

Query: 241 ITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYI 300
           ITSR LTALVMAA  GP   +  FRLKS  RKLLRHQML NGGLERVGEQLE AAKGSYI
Sbjct: 241 ITSRGLTALVMAADQGPVLRY--FRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+C VTINR+R  VINQM
Sbjct: 361 EEHLYLCFVTINRSRASVINQM 357

BLAST of Spg008550 vs. NCBI nr
Match: XP_023542867.1 (UPF0496 protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.1 bits (1315), Expect = 8.0e-141
Identity = 281/382 (73.56%), Postives = 309/382 (80.89%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDEY 60
           MW KFRAS+                        + I  FAD   QIRNK Q+SFNVN+EY
Sbjct: 1   MWTKFRASRF-----------------------IWISVFADVKHQIRNKPQRSFNVNEEY 60

Query: 61  LCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILESPL 120
           LC LR++S++EFF K Q IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILESPL
Sbjct: 61  LCALRSRSFVEFFAKAQLIVQESPPSTSSSADHYCRKFSETILLQSDQLEANPSILESPL 120

Query: 121 LLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIET 180
           L+MLPEL+ LLID+FNVSAEASNLC RLLANLK ARSNSRF+Q+ LDSIEKC S ++IET
Sbjct: 121 LVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEIET 180

Query: 181 IASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIA 240
           I SDLLAQRAP SD+DKRDFA IHD Y AVS  LN +RKKVARKIRSIKIIDR+TCGL+A
Sbjct: 181 ITSDLLAQRAPLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGLVA 240

Query: 241 ITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYI 300
           ITSR LTALV+AA  GP   +  FRLKS  RKLL+HQML NGGLERVGEQLE AAKGSYI
Sbjct: 241 ITSRGLTALVLAADQGPVLRY--FRLKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 357

BLAST of Spg008550 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 2.9e-24
Identity = 101/359 (28.13%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 55  NVNDEYLCTLRTKSYIEFFTKVQ--------SIVHESPPSTSSSSTDLRRKFSETILLQP 114
           +VN+EY    RT SY+E  TK +        S +  S PS SSSS           LL P
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLDP 94

Query: 115 GQLEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSL 174
            Q E + ++++         L +L++ +F++S+EA ++C  LL  L+  + N   I++ +
Sbjct: 95  PQ-ETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 175 DSIEK-CSSAEKIETIASDL-------LAQRAPFSD-----IDKRDFALIHDEYTAVSYR 234
              ++ C+ A+ +E     L       L++ A   +     +++  F ++HD  + +  +
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 235 LNCSRKKVARKIRSIKIIDRFTCGLIAIT--SRTLTALVMA----AGPGPGPAHFGF--- 294
           L   ++++ RKIR  K   +     + IT  +  +T L++A     G    PA  G    
Sbjct: 215 LTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 274

Query: 295 -RLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGK 354
             L+    K   H+  ++  LE++G Q++ AAKG +IL  +LDT SRL  RL D ++H K
Sbjct: 275 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 334

Query: 355 AMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            +  +  + ++ +  +  A+ E          Q++++EEHLY+C  TINR+R LV+ Q+
Sbjct: 335 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of Spg008550 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 6.4e-24
Identity = 109/360 (30.28%), Postives = 174/360 (48.33%), Query Frame = 0

Query: 53  SFNVNDEYLCTLRTKSYIEFFTKVQSIV----------HESPPSTSSSSTDLRRKFSETI 112
           SF+  +EY    RT+SY +F+ +V  I           H      ++S      +     
Sbjct: 31  SFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEH 90

Query: 113 LLQPGQLEAIPSILESPLLLML-PELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRF 172
           LL+P Q  A+ + L SP    L P+++ LL  Y+  +A AS LCS LL +++  R   R 
Sbjct: 91  LLEPDQ-RAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRP 150

Query: 173 IQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFAL--IHDEYTAVSYRLNCSRK 232
           ++ +L    K +S   +  +A    A   PF+ +      L  +      +   L+  RK
Sbjct: 151 LKHTL---RKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRK 210

Query: 233 KVARKIRSIKIIDRFTCGLIAITSRTLTALV-----------MAAGPGPGPAHFGFRLKS 292
           K   +IRS+  + R    +  +T+  + A+V            AA P   PA  G R   
Sbjct: 211 KARHRIRSVARLRR-ALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERF-- 270

Query: 293 FSRKLLRHQMLRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMVRL 352
           FS +  R  ++          QLE AAKG+YILNR+++T SRLV R+ D  +H  A++RL
Sbjct: 271 FSGRAARRALV----------QLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRL 330

Query: 353 FVERKEDKFA------VAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            VE +    A      V   + +L K+  S R+Q++++EEHL++C +TIN+ R +V+N M
Sbjct: 331 CVEHRPAAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVMNFM 373

BLAST of Spg008550 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 7.0e-23
Identity = 108/360 (30.00%), Postives = 172/360 (47.78%), Query Frame = 0

Query: 53  SFNVNDEYLCTLRTKSYIEFFTKVQSIV----------HESPPSTSSSSTDLRRKFSETI 112
           SF+  +EY    RT+SY +F+ +V  I           H      ++S      +     
Sbjct: 31  SFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEH 90

Query: 113 LLQPGQLEAIPSILESPLLLML-PELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRF 172
           LL+P Q  A+ + L SP    L P+++ LL  Y+  +A AS LCS LL +++  R   R 
Sbjct: 91  LLEPDQ-RAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRP 150

Query: 173 IQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFAL--IHDEYTAVSYRLNCSRK 232
           ++ +L    K +S   +  +A    A   PF+ +      L  +      +   L+  RK
Sbjct: 151 LKHTL---RKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRK 210

Query: 233 KVARKIRSIKIIDRFTCGLIAITSRTLTALV-----------MAAGPGPGPAHFGFRLKS 292
           K   +IRS+  + R    +  +T+  + A+V            AA P   PA  G R   
Sbjct: 211 KARHRIRSVARLRR-ALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERF-- 270

Query: 293 FSRKLLRHQMLRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMVRL 352
           FS +  R  ++          QLE AAKG+YILNR+++T SRLV R+ D  +H  A+ RL
Sbjct: 271 FSGRAARRALV----------QLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRL 330

Query: 353 FVERKEDKFA------VAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            VE +    A      V   + +L K+  S R+Q++++EEHL++C +T N+ R +V+N M
Sbjct: 331 CVEHRPAAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVMNFM 373

BLAST of Spg008550 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-21
Identity = 108/351 (30.77%), Postives = 167/351 (47.58%), Query Frame = 0

Query: 49  KSQKSFNVNDEYLCTLRTKSYIEFFTKVQSIVHE--------SPPSTSSSSTDLR----R 108
           K     +V +EY    RT+SY  F+T+V  +  +        S P   SSST  R    R
Sbjct: 64  KDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYR 123

Query: 109 KFSETILLQPGQLEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSAR 168
            F+   LL P  L  I  IL+  +  +    + LL DYF  +A A  LC++LL N+   R
Sbjct: 124 LFAHN-LLDP-DLNTITRILD--VSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLR 183

Query: 169 SNSRFIQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNC 228
           S    ++    S E  +S   I+   +++     PF     R   LI      +  RL  
Sbjct: 184 SKYESLKPKFHS-ENHNSLALIDQF-TEISKWFDPFISSGSR-IQLIRSGCLYLLKRLES 243

Query: 229 SRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRH 288
            R K   K++ I  +   +  L+   + TL   +         A   F L   +  LL  
Sbjct: 244 RRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTI---------ASHAFALFLAAPTLLAS 303

Query: 289 QM----LRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVER 348
           Q     LRN  L +   +L+ AAKG+YIL+R+LDT SRLV R+ D V+H +AM   +V R
Sbjct: 304 QFKPAGLRN-KLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGR 363

Query: 349 KEDKFAVAVAM-DELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
              +   +  +  ELK+   S  ++++++EEH+Y+C +TINR R L++ ++
Sbjct: 364 GSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of Spg008550 vs. ExPASy Swiss-Prot
Match: A2YH25 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 PE=3 SV=2)

HSP 1 Score: 101.3 bits (251), Expect = 2.5e-20
Identity = 74/264 (28.03%), Postives = 140/264 (53.03%), Query Frame = 0

Query: 127 LKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIETIASDLL 186
           ++ LLI+YF+V+ EA   CS LLA + +AR +   +++ L  ++     +  + +A  + 
Sbjct: 123 VETLLIEYFDVTEEACEACSALLAAIGAARRHHLTLRRLLLRLDGGDDDDAKDALARHVR 182

Query: 187 AQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLI--AITSR 246
               P S     +F  +H   + ++ RL  +++++ R  R+++I        +  A  + 
Sbjct: 183 LDN-PLSPGSLSEFHDVHARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAA 242

Query: 247 TLTALVMAA----GPGPGPAHFGFRLKSFSRKLLRH--QMLRNGGLERVGEQLEEAAKGS 306
            + A+V+AA    G G   A FG      +R   R   + + +    R G  L+ AA+G+
Sbjct: 243 IVAAVVLAAHALVGIGVAAAAFGATPAGAARWWARRAAEKVSSRHYARAGATLDAAARGA 302

Query: 307 YILNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVE 366
           YI+ R+LDT SR+V R  D ++HG+ + R+ +    ++  +     E ++    +R Q+ 
Sbjct: 303 YIVGRDLDTVSRMVRRAHDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLA 362

Query: 367 DVEEHLYMCIVTINRTRTLVINQM 383
           ++EEH+ +C++TINRTR LV ++M
Sbjct: 363 ELEEHVCLCLITINRTRRLVAHEM 385

BLAST of Spg008550 vs. ExPASy TrEMBL
Match: A0A6J1H6T3 (UPF0496 protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460642 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.6e-142
Identity = 285/382 (74.61%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDEY 60
           MW KFRAS+                        + I  FAD   QIRNK QKSFNVN+EY
Sbjct: 1   MWTKFRASRF-----------------------IWISVFADVKHQIRNKPQKSFNVNEEY 60

Query: 61  LCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILESPL 120
           LC LR++S++EFF K Q IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILESPL
Sbjct: 61  LCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILESPL 120

Query: 121 LLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIET 180
           L+MLPEL+ LLID+FNVSAEASNLC RLLANLK ARSNSRF+Q+ LDSIEKCSS ++IET
Sbjct: 121 LVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIET 180

Query: 181 IASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIA 240
           I SDLLAQRAP SD+DKRDFA IHD+Y AVS  LN +RKKVARKIRSIKIIDRFTCGL+A
Sbjct: 181 ITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGLVA 240

Query: 241 ITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYI 300
           ITSR LTALVMAA  GP   +  FRLKS  RKLLRHQML NGGLERVGEQLE AAKGSYI
Sbjct: 241 ITSRGLTALVMAADQGPVLRY--FRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDHGKAMVRLFVERKE+KFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKENKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 357

BLAST of Spg008550 vs. ExPASy TrEMBL
Match: A0A6J1JMU4 (UPF0496 protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111487265 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 9.5e-140
Identity = 282/382 (73.82%), Postives = 309/382 (80.89%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDEY 60
           MW KFRAS+                        + I  FAD   QIRNK QKSFNVN+EY
Sbjct: 1   MWTKFRASRF-----------------------IWISVFADVKHQIRNKPQKSFNVNEEY 60

Query: 61  LCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILESPL 120
           LC LR++S++EFF K Q IV ESPPSTSS+     RKFSETILLQ  QLEA PSILESPL
Sbjct: 61  LCALRSRSFVEFFAKAQLIVQESPPSTSSTGHHC-RKFSETILLQIDQLEANPSILESPL 120

Query: 121 LLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIET 180
           L+MLPEL+ LLID+FNVSAEASNLC RLLANLK ARSNSRF+Q+ LDSIEKCSS ++IET
Sbjct: 121 LVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEIET 180

Query: 181 IASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIA 240
           I SDLLAQRAP SD+DKRDFA IHD+Y AVS  LN +RKKVARKIRSIKII++ TCGL+A
Sbjct: 181 ITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIINKCTCGLVA 240

Query: 241 ITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYI 300
           ITSR LTALVMAA  GP   +  FRLKS  RKLLRHQML NGGLERVGEQLE AAKGSYI
Sbjct: 241 ITSRGLTALVMAADQGPVLRY--FRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 356

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 356

BLAST of Spg008550 vs. ExPASy TrEMBL
Match: A0A6J1BWZ5 (UPF0496 protein 3-like OS=Momordica charantia OX=3673 GN=LOC111006097 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.4e-135
Identity = 272/385 (70.65%), Postives = 303/385 (78.70%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCS-ENLCNFSQPVLIPDFADGMQQIRNKSQKSFNVNDE 60
           MW K R  KIRN T +  SI  CS   L NFSQPVLI  FAD  QQIRNK QKSFNVNDE
Sbjct: 1   MWPKIRDLKIRNSTHISSSIFACSAAQLLNFSQPVLISAFADAKQQIRNKPQKSFNVNDE 60

Query: 61  YLCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTD--LRRKFSETILLQPGQLEAIPSILE 120
           Y+C LRTKSY+EFF K QS++ ES P ++SSST    RRK S TILL+P Q EAIPSILE
Sbjct: 61  YICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSGTILLEPSQ-EAIPSILE 120

Query: 121 SPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEK 180
           SP LLMLP+LK LLIDYFNVSAEAS  C+RLL +++  RSNSR IQ+SLDSIE CSS E 
Sbjct: 121 SPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPET 180

Query: 181 IETIASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCG 240
           IETIAS+ LA R PFSD DK DFALIHD+Y AVS RLNC+RKKVARKI+SI+II+  +CG
Sbjct: 181 IETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISCG 240

Query: 241 LIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKG 300
           L+AIT+RTLT L            F F  +SF RKLLR+QM+RNGGL  VGEQLE AAKG
Sbjct: 241 LVAITARTLTEL------------FRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKG 300

Query: 301 SYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQV 360
           SYILNRE DTTSRLV RL DA+DHGKAM RLFVERKEDKFAV VAMDELKKSNL +R QV
Sbjct: 301 SYILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQV 360

Query: 361 EDVEEHLYMCIVTINRTRTLVINQM 383
           E+VEEHLY+CIVTINR R LVINQ+
Sbjct: 361 EEVEEHLYLCIVTINRARGLVINQI 372

BLAST of Spg008550 vs. ExPASy TrEMBL
Match: A0A5D3DGL5 (UPF0496 protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00820 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 1.1e-127
Identity = 260/384 (67.71%), Postives = 304/384 (79.17%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQ-IRNKSQKSFNVNDE 60
           MW KF  SKI N TKL  SIL CS              FAD   + IR K ++SFNVN+E
Sbjct: 1   MWTKFSPSKIINSTKLSQSILACS-------------TFADQEHEIIRKKHKRSFNVNEE 60

Query: 61  YLCTLRTKSYIEFFTKVQSIVHESPPSTSSSST--DLRRKFSETILLQPGQLEAIPSILE 120
           YLCTLRT+S+ EFF K +S VHESPPST+SSS+  D   +FSETILLQPGQLEA+PSILE
Sbjct: 61  YLCTLRTESFAEFFLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSILE 120

Query: 121 SPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEK 180
           S  LLMLPELK L +DYFN+SA+AS+LC+RLLAN K  RS SR IQ+SLDSIEKC S+E 
Sbjct: 121 SSFLLMLPELKGLFVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSSET 180

Query: 181 IETIASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCG 240
           +E+IAS+LLA R+PFSD++KRDFALIHD+YT +S+RLNC+RKKVARKIRS+KI+D  TCG
Sbjct: 181 VESIASNLLALRSPFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGITCG 240

Query: 241 LIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKG 300
           L AIT+RTLT LV AA  GPG          F RKLLRH+MLRNGGLE+VGE+LE AAKG
Sbjct: 241 LNAITTRTLTDLVKAADRGPG---------VFGRKLLRHEMLRNGGLEKVGEKLEAAAKG 300

Query: 301 SYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVER-KEDKFAVAVAMDELKKSNLSIRKQ 360
           SYIL REL+TTSRLVVRLGDAVD+GKAMVRLF  R KEDKF V VAMDE+KK+N +IRK+
Sbjct: 301 SYILKRELETTSRLVVRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKR 360

Query: 361 VEDVEEHLYMCIVTINRTRTLVIN 381
           VEDVEEHL +CIV INR++  +IN
Sbjct: 361 VEDVEEHLCLCIVAINRSKASMIN 362

BLAST of Spg008550 vs. ExPASy TrEMBL
Match: A0A1S3B3L4 (UPF0496 protein 3-like OS=Cucumis melo OX=3656 GN=LOC103485615 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 1.1e-127
Identity = 260/384 (67.71%), Postives = 304/384 (79.17%), Query Frame = 0

Query: 1   MWAKFRASKIRNRTKLFPSILDCSENLCNFSQPVLIPDFADGMQQ-IRNKSQKSFNVNDE 60
           MW KF  SKI N TKL  SIL CS              FAD   + IR K ++SFNVN+E
Sbjct: 1   MWTKFSPSKIINSTKLSQSILACS-------------TFADQEHEIIRKKHKRSFNVNEE 60

Query: 61  YLCTLRTKSYIEFFTKVQSIVHESPPSTSSSST--DLRRKFSETILLQPGQLEAIPSILE 120
           YLCTLRT+S+ EFF K +S VHESPPST+SSS+  D   +FSETILLQPGQLEA+PSILE
Sbjct: 61  YLCTLRTESFAEFFLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSILE 120

Query: 121 SPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSLDSIEKCSSAEK 180
           S  LLMLPELK L +DYFN+SA+AS+LC+RLLAN K  RS SR IQ+SLDSIEKC S+E 
Sbjct: 121 SSFLLMLPELKGLFVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSSET 180

Query: 181 IETIASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNCSRKKVARKIRSIKIIDRFTCG 240
           +E+IAS+LLA R+PFSD++KRDFALIHD+YT +S+RLNC+RKKVARKIRS+KI+D  TCG
Sbjct: 181 VESIASNLLALRSPFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGITCG 240

Query: 241 LIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKG 300
           L AIT+RTLT LV AA  GPG          F RKLLRH+MLRNGGLE+VGE+LE AAKG
Sbjct: 241 LNAITTRTLTDLVKAADRGPG---------VFGRKLLRHEMLRNGGLEKVGEKLEAAAKG 300

Query: 301 SYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVER-KEDKFAVAVAMDELKKSNLSIRKQ 360
           SYIL REL+TTSRLVVRLGDAVD+GKAMVRLF  R KEDKF V VAMDE+KK+N +IRK+
Sbjct: 301 SYILKRELETTSRLVVRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKR 360

Query: 361 VEDVEEHLYMCIVTINRTRTLVIN 381
           VEDVEEHL +CIV INR++  +IN
Sbjct: 361 VEDVEEHLCLCIVAINRSKASMIN 362

BLAST of Spg008550 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 114.4 bits (285), Expect = 2.0e-25
Identity = 101/359 (28.13%), Postives = 179/359 (49.86%), Query Frame = 0

Query: 55  NVNDEYLCTLRTKSYIEFFTKVQ--------SIVHESPPSTSSSSTDLRRKFSETILLQP 114
           +VN+EY    RT SY+E  TK +        S +  S PS SSSS           LL P
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLDP 94

Query: 115 GQLEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSL 174
            Q E + ++++         L +L++ +F++S+EA ++C  LL  L+  + N   I++ +
Sbjct: 95  PQ-ETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 175 DSIEK-CSSAEKIETIASDL-------LAQRAPFSD-----IDKRDFALIHDEYTAVSYR 234
              ++ C+ A+ +E     L       L++ A   +     +++  F ++HD  + +  +
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 235 LNCSRKKVARKIRSIKIIDRFTCGLIAIT--SRTLTALVMA----AGPGPGPAHFGF--- 294
           L   ++++ RKIR  K   +     + IT  +  +T L++A     G    PA  G    
Sbjct: 215 LTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 274

Query: 295 -RLKSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGK 354
             L+    K   H+  ++  LE++G Q++ AAKG +IL  +LDT SRL  RL D ++H K
Sbjct: 275 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 334

Query: 355 AMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            +  +  + ++ +  +  A+ E          Q++++EEHLY+C  TINR+R LV+ Q+
Sbjct: 335 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of Spg008550 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-22
Identity = 108/351 (30.77%), Postives = 167/351 (47.58%), Query Frame = 0

Query: 49  KSQKSFNVNDEYLCTLRTKSYIEFFTKVQSIVHE--------SPPSTSSSSTDLR----R 108
           K     +V +EY    RT+SY  F+T+V  +  +        S P   SSST  R    R
Sbjct: 64  KDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYR 123

Query: 109 KFSETILLQPGQLEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSAR 168
            F+   LL P  L  I  IL+  +  +    + LL DYF  +A A  LC++LL N+   R
Sbjct: 124 LFAHN-LLDP-DLNTITRILD--VSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLR 183

Query: 169 SNSRFIQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFALIHDEYTAVSYRLNC 228
           S    ++    S E  +S   I+   +++     PF     R   LI      +  RL  
Sbjct: 184 SKYESLKPKFHS-ENHNSLALIDQF-TEISKWFDPFISSGSR-IQLIRSGCLYLLKRLES 243

Query: 229 SRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRH 288
            R K   K++ I  +   +  L+   + TL   +         A   F L   +  LL  
Sbjct: 244 RRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTI---------ASHAFALFLAAPTLLAS 303

Query: 289 QM----LRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVER 348
           Q     LRN  L +   +L+ AAKG+YIL+R+LDT SRLV R+ D V+H +AM   +V R
Sbjct: 304 QFKPAGLRN-KLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGR 363

Query: 349 KEDKFAVAVAM-DELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
              +   +  +  ELK+   S  ++++++EEH+Y+C +TINR R L++ ++
Sbjct: 364 GSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of Spg008550 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 102.8 bits (255), Expect = 6.1e-22
Identity = 93/349 (26.65%), Postives = 169/349 (48.42%), Query Frame = 0

Query: 55  NVNDEYLCTLRTKSYIEFFTKVQ--------SIVHESPPSTSSSSTDLRRKFSETILLQP 114
           +VN+EY    RT SY+E  TK +        S +  S PS SSSS           LL P
Sbjct: 35  SVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLDP 94

Query: 115 GQLEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSL 174
            Q E + ++++         L +L++ +F++S+EA ++C  LL  L+  + N   I++ +
Sbjct: 95  PQ-ETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVM 154

Query: 175 DSIEK-CSSAEKIETIASDL-------LAQRAPFSD-----IDKRDFALIHDEYTAVSYR 234
              ++ C+ A+ +E     L       L++ A   +     +++  F ++HD  + +  +
Sbjct: 155 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 214

Query: 235 LNCSRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKL 294
           L   ++++ RKI  + +       L+ + S  L                   L+    K 
Sbjct: 215 LTSKKRRIRRKISILGVF--AAPALLGLCSFCL-------------------LRKKKAKG 274

Query: 295 LRHQMLRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVERK 354
             H+  ++  LE++G Q++ AAKG +IL  +LDT SRL  RL D ++H K +  +  + +
Sbjct: 275 RMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAMCAKSR 334

Query: 355 EDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
           + +  +  A+ E          Q++++EEHLY+C  TINR+R LV+ Q+
Sbjct: 335 KIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 354

BLAST of Spg008550 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-17
Identity = 89/356 (25.00%), Postives = 159/356 (44.66%), Query Frame = 0

Query: 50  SQKSFNVNDEYLCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQ- 109
           S  +FN++ E     +T SY +  ++V  +V  +         D+    S+  +LQP + 
Sbjct: 41  SSPTFNLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQ--VLQPNKE 100

Query: 110 --LEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSL 169
              EAI  + ++        L +L+  YF  S +A+ LC  L  N+ SAR +       L
Sbjct: 101 CVQEAIRHVKQT-------TLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDL 160

Query: 170 DSIEKCSSAEKIETIASDLLAQ--------RAPFSDIDKRDFALIHDEYTAVSYRLNCSR 229
            +I    S   I+    DL             PFS  +   F      ++ + + L+   
Sbjct: 161 FNIFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRL 220

Query: 230 KKVARKIRSI------------KIIDRFTCGLIAITSRTLTALVMAAGPGPGPAHFGFRL 289
           +K   ++R I              +       + I S  L  L++ AGP   P    +  
Sbjct: 221 RKSRSRVRLIHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSP----YLP 280

Query: 290 KSFSRKLLRHQMLRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMV 349
            SF RK L +             QL  A+KG+++LN++LDT  RLV RL   +++ K ++
Sbjct: 281 HSFKRKELTNIC-----------QLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLI 340

Query: 350 RLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
           RL +ER  D  ++   +  L+KS+L +  Q++D+E+H+ +    +N+ R+L++ ++
Sbjct: 341 RLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 372

BLAST of Spg008550 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 79.0 bits (193), Expect = 9.5e-15
Identity = 85/344 (24.71%), Postives = 151/344 (43.90%), Query Frame = 0

Query: 50  SQKSFNVNDEYLCTLRTKSYIEFFTKVQSIVHESPPSTSSSSTDLRRKFSETILLQPGQ- 109
           S  +FN++ E     +T SY +  ++V  +V  +         D+    S+  +LQP + 
Sbjct: 41  SSPTFNLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQ--VLQPNKE 100

Query: 110 --LEAIPSILESPLLLMLPELKDLLIDYFNVSAEASNLCSRLLANLKSARSNSRFIQQSL 169
              EAI  + ++        L +L+  YF  S +A+ LC  L  N+ SAR +       L
Sbjct: 101 CVQEAIRHVKQT-------TLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDL 160

Query: 170 DSIEKCSSAEKIETIASDLLAQ--------RAPFSDIDKRDFALIHDEYTAVSYRLNCSR 229
            +I    S   I+    DL             PFS  +   F      ++ + + L+   
Sbjct: 161 FNIFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRL 220

Query: 230 KKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPGPAHFGFRLKSFSRKLLRHQM 289
           +K   ++R I                        AGP   P    +   SF RK L +  
Sbjct: 221 RKSRSRVRLIH--------------------HATAGPLCSP----YLPHSFKRKELTNIC 280

Query: 290 LRNGGLERVGEQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFA 349
                      QL  A+KG+++LN++LDT  RLV RL   +++ K ++RL +ER  D  +
Sbjct: 281 -----------QLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHS 340

Query: 350 VAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
           +   +  L+KS+L +  Q++D+E+H+ +    +N+ R+L++ ++
Sbjct: 341 IQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340