Spg008507 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008507
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	flavonol synthase/flavanone 3-hydroxylase-like
Location	scaffold10: 34645933 .. 34647559 (-)
RNA-Seq Expression	Spg008507
Synteny	Spg008507

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGTTGAAACCGTATTGATTGAAACGACGAAGCAAAAAGCCAACAAGAACATGTAGCTGTAGCCTCTGTTCTTTAATTAGGGCTGGTAGTTCTATCCAACGATCCTAAACTTGTCCACGTTTCTTCTTCATTCATTCTCCATTCTCCAACCATTCCCAGATCCCACTTTTAAACTCACCCAAATCGGAATCTGTGCTCCATTGCCACCAGAAATGGAGGTCGGGAGAGTCCAGGACATCGCCTCGGCGGTGCTCTTCTCCGGCAGTCTCCCGCCCGAGTTCGTCCGCCCGGAGACCGAGCAGCCCGGAATCACCACCGTCAAAGGCCAAACCCTCGCCGTTCCCGTCATCGATTTCAGCAACCCAGATGAGGACTCTGTAATTCGAGCCATCGCCGACGCCAGTCGCCATTGGGGCATGTTTCAAATCGTCAACCACGAGATCCCAAACGAAGCGATCGCCCATTTGCAGGCTGTGGGGAAGGAATTCTTCGAGCTCTCACCAGAGGAGAAAGAGGTTTACGCTAAGCCCGCTGGTTCCAAATCCGTCGAAGGCTACGGAACCAAGCTCCAGAAGGAAGTCGATGGAAAGAAAGGCTGGGTCGACCATTTGTTCCATATCATCTGGCCTCCTTCTGCCATTAACTACAAATTCTGGCCTCGAACCCCTCCTTCTTACAGGTATGGAGATGAGAACAAAAAAATCTCGCCAAATTCCATTACGTTTCTTGATCGTTGAGTTTTCTGGGTCTGAATACAGGGCTGCGAACGAGGAGTACAGAAAGCACTTACAAGGGGTTGTGGATAAGCTCTTCAAAAGCCTCTCGCTCGGGCTGGGGCTCGAAGGGCATGAACTGAAAGAGCGCGCTGGTGGAGATGAATTAGTCTATTTACTGAAGATCAATTACTACCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCACACACCGACATGTCGACCATTACAATTCTTGTTCCGAATGAAGTTCAAGGCCTCCAGGCCTTCAGAGATGGGCATTGGTACGATGTCAATTACATCCCCAATGCCCTCGTCATTCACATTGGCGATCAAATTGAGGTACTTAAAAGTCGTTTTCAACTCCATGGATTTGCAGGTGGTTCTGCTGTGAAAAGGGCTTGATTAGAACTGGTTTTTTGTTCTGTGTGTGAGCAGATTCTGAGTAATGGGAAGTACAAGAGTGTGTTGCACAGGACGACTGTGAACAAGGACAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCGCCGGCAGATTTTGAAGTTGGGCCTCACCCGAAACTGGTGGATGAAGAGAATCCGGCGAAGTATAAGACGAAGAAGTACGGTGAGTATGCATATTGCAAGTTGAATAAGATTCCACAGTGACTGTGACAGCGCCTGAGATTCTGAGATTCTTCTGCAGCTTCTTCCTTGGTCGTTTGTTTCGTCGTCTCTGGGCTTAGTGGTTTATAAAGGCTCAAGTTTATGTAATTTAGCATATGGCAACGAGGGTTTGTGTTATTTTCAATGAATAACGTACTCTGTTTTAGATTGATGCTTCTCGGTAGAAATCAAACAACATAATATTAGTATTGAAAGTTTGATTAAATGAAGAAGG

mRNA sequence

ATGGAGGTCGGGAGAGTCCAGGACATCGCCTCGGCGGTGCTCTTCTCCGGCAGTCTCCCGCCCGAGTTCGTCCGCCCGGAGACCGAGCAGCCCGGAATCACCACCGTCAAAGGCCAAACCCTCGCCGTTCCCGTCATCGATTTCAGCAACCCAGATGAGGACTCTGTAATTCGAGCCATCGCCGACGCCAGTCGCCATTGGGGCATGTTTCAAATCGTCAACCACGAGATCCCAAACGAAGCGATCGCCCATTTGCAGGCTGTGGGGAAGGAATTCTTCGAGCTCTCACCAGAGGAGAAAGAGGTTTACGCTAAGCCCGCTGGTTCCAAATCCGTCGAAGGCTACGGAACCAAGCTCCAGAAGGAAGTCGATGGAAAGAAAGGCTGGGTCGACCATTTGTTCCATATCATCTGGCCTCCTTCTGCCATTAACTACAAATTCTGGCCTCGAACCCCTCCTTCTTACAGGGCTGCGAACGAGGAGTACAGAAAGCACTTACAAGGGGTTGTGGATAAGCTCTTCAAAAGCCTCTCGCTCGGGCTGGGGCTCGAAGGGCATGAACTGAAAGAGCGCGCTGGTGGAGATGAATTAGTCTATTTACTGAAGATCAATTACTACCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCACACACCGACATGTCGACCATTACAATTCTTGTTCCGAATGAAGTTCAAGGCCTCCAGGCCTTCAGAGATGGGCATTGGTACGATGTCAATTACATCCCCAATGCCCTCGTCATTCACATTGGCGATCAAATTGAGATTCTGAGTAATGGGAAGTACAAGAGTGTGTTGCACAGGACGACTGTGAACAAGGACAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCGCCGGCAGATTTTGAAGTTGGGCCTCACCCGAAACTGGTGGATGAAGAGAATCCGGCGAAGTATAAGACGAAGAAGTACGGTGAGTATGCATATTGCAAGTTGAATAAGATTCCACAGTGA

Coding sequence (CDS)

ATGGAGGTCGGGAGAGTCCAGGACATCGCCTCGGCGGTGCTCTTCTCCGGCAGTCTCCCGCCCGAGTTCGTCCGCCCGGAGACCGAGCAGCCCGGAATCACCACCGTCAAAGGCCAAACCCTCGCCGTTCCCGTCATCGATTTCAGCAACCCAGATGAGGACTCTGTAATTCGAGCCATCGCCGACGCCAGTCGCCATTGGGGCATGTTTCAAATCGTCAACCACGAGATCCCAAACGAAGCGATCGCCCATTTGCAGGCTGTGGGGAAGGAATTCTTCGAGCTCTCACCAGAGGAGAAAGAGGTTTACGCTAAGCCCGCTGGTTCCAAATCCGTCGAAGGCTACGGAACCAAGCTCCAGAAGGAAGTCGATGGAAAGAAAGGCTGGGTCGACCATTTGTTCCATATCATCTGGCCTCCTTCTGCCATTAACTACAAATTCTGGCCTCGAACCCCTCCTTCTTACAGGGCTGCGAACGAGGAGTACAGAAAGCACTTACAAGGGGTTGTGGATAAGCTCTTCAAAAGCCTCTCGCTCGGGCTGGGGCTCGAAGGGCATGAACTGAAAGAGCGCGCTGGTGGAGATGAATTAGTCTATTTACTGAAGATCAATTACTACCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCACACACCGACATGTCGACCATTACAATTCTTGTTCCGAATGAAGTTCAAGGCCTCCAGGCCTTCAGAGATGGGCATTGGTACGATGTCAATTACATCCCCAATGCCCTCGTCATTCACATTGGCGATCAAATTGAGATTCTGAGTAATGGGAAGTACAAGAGTGTGTTGCACAGGACGACTGTGAACAAGGACAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCGCCGGCAGATTTTGAAGTTGGGCCTCACCCGAAACTGGTGGATGAAGAGAATCCGGCGAAGTATAAGACGAAGAAGTACGGTGAGTATGCATATTGCAAGTTGAATAAGATTCCACAGTGA

Protein sequence

MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAIADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQKEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLGLGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADFEVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ

Homology

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BLAST of Spg008507 vs. NCBI nr
Match: KAG6578981.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 303/335 (90.45%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGSLP EFVRP+TEQPGITTVKGQ LAVPVIDF NPDEDSVI+AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDGKKGWVDHLFHIIWPPSAIN+KFWP+ PPSYRAANEEYRKHL GVVDKLFKSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFKSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

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BLAST of Spg008507 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 303/335 (90.45%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGSLP EFVRP+TEQPGITTVKGQ LAVPVIDF NPDEDSVIRAI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDGKKGWVDHLFHIIWPPSAIN+KFWP+ PPSYRAANEEYRKHL GVVDKLF+SLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

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BLAST of Spg008507 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.0 bits (1660), Expect = 6.9e-181
Identity = 302/335 (90.15%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGSLP EFVRP+TEQPGITTVKGQ LAVPVIDF NPDEDSVI+AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDGKKGWVDHLFHIIWPPSAIN+KFWP+ PPSYRAANEEYRKHL GVVDKLF+SLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

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BLAST of Spg008507 vs. NCBI nr
Match: XP_022938382.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 642.9 bits (1657), Expect = 1.5e-180
Identity = 301/335 (89.85%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGSLP EFVRP+TEQPGITTVKGQ LAVPVIDF NPDEDSVI+AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDG+KGWVDHLFHIIWPPSAIN+KFWP+ PPSYRAANEEYRKHL GVVDKLF+SLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Spg008507 vs. NCBI nr
Match: XP_022993310.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 638.3 bits (1645), Expect = 3.8e-179
Identity = 298/335 (88.96%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGS+P EFVRP+ EQPGITTVKGQ LAVPVIDF NPDEDSVI+AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDGKKGWVDHLFHIIWPPSAIN+KFWP+ PPSYR ANEEYRKHL GVVDKLF+SLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELK+ AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

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BLAST of Spg008507 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 5.6e-149
Identity = 243/336 (72.32%), Postives = 282/336 (83.93%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEV RVQ IAS      ++P E++R E EQP  TT+ G  L VPVID  +PDE+ +++ I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSP-EEKEVYAKPAGSKSVEGYGTKL 120
           ADAS+ WG+FQ++NH IP+EAIA LQ VGKEFFE  P EEKE+ AK  GS  +EGYGT L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 QKEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSL 180
           QKEV+GKKGWVDHLFH IWPPSA+NY++WP+ PPSYR ANEEY K ++ VVD++FKSLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQ 240
           GLGLEGHE+ E AGGDE+VYLLKINYYPPCPRPDLALGV AHTDMS ITILVPNEVQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 AFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPAD 300
            F+DGHWYDV YIPNAL++HIGDQ+EILSNGKYKSV HRTTVNKDKTR+SWPVFLEPP++
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FEVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
            EVGP PKL+ E NP K+KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

[Close]

BLAST of Spg008507 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 4.4e-146
Identity = 240/335 (71.64%), Postives = 279/335 (83.28%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEV RVQ+IAS      ++P E++R E EQP I+TV G  L VPVID S+ DE  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
           ++AS+ WG+FQ+VNH IPNE I  LQ VGK FFEL  EEKE+ AKP GS+S+EGYGT+LQ
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDGKKGWVDHLFH IWPPSAINY+FWP+ PP+YR ANEEY K LQ VVD LFK LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           L LE +  K+ AGGD+LVYL+KINYYPPCPRPDLALGV AHTDMS IT+LVPNEV GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           ++DGHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV HRTTVNK+KTR+SWPVFLEPP D 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGP PKLV+EENPAK+KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

[Close]

BLAST of Spg008507 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 6.4e-145
Identity = 239/323 (73.99%), Postives = 273/323 (84.52%), Query Frame = 0


Query: 17  GSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAIADASRHWGMFQIVNHE 76
           G++P EF+R E EQPGITTV G+ L VP+IDFS+PDE+ +I  I +AS +WGM+QIVNH+
Sbjct: 15  GTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHD 74

Query: 77  IPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQKEVD----GKKGWVDH 136
           IP+E I+ LQAVGKEFFEL  EEKE YAKP  S S+EGYGTKL KE+      KKGWVD+
Sbjct: 75  IPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDN 134

Query: 137 LFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLGLGLEGHELKERA 196
           LF+ IWPPS +NY+FWP+ PPSYR ANEEY KHL  VV+KLF+ LSLGLGLEG ELK+ A
Sbjct: 135 LFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAA 194

Query: 197 GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQAFRDGHWYDVNYI 256
           GGD L YLLKINYYPPCPRPDLALGV AHTDMST+TILVPN+VQGLQA +DG WYDV YI
Sbjct: 195 GGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYI 254

Query: 257 PNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADFEVGPHPKLVDEE 316
           PNALVIHIGDQ+EI+SNGKY SVLHRTTVNKDKTRISWPVFLEPPAD  VGPHP+LV+  
Sbjct: 255 PNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAV 314

Query: 317 NPAKYKTKKYGEYAYCKLNKIPQ 336
           N  KYKTKKYG+Y YCK+NK+PQ
Sbjct: 315 NQPKYKTKKYGDYVYCKINKLPQ 337

[Close]

BLAST of Spg008507 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 3.2e-144
Identity = 234/335 (69.85%), Postives = 277/335 (82.69%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEV RVQ IAS    +G++P EF+RPE EQP  TT  G    +P ID  +P +D ++R+I
Sbjct: 1   MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
           A+ASR WG+FQ+ NH IP++ I  LQAVGKEFFEL  EEKEVY++PA +K V+GYGTKLQ
Sbjct: 61  AEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEV+GKK WVDHLFH +WPPS+INY+FWP+ PPSYRA NEEY K+++ VVDKLF  LSLG
Sbjct: 121 KEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LG+EG  LKE AGGD++ Y+LKINYYPPCPRPDLALGV AHTD+S +T+LVPNEV GLQ 
Sbjct: 181 LGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           F+D  W D  YIPNALVIHIGDQIEILSNGKYK+VLHRTTVNKDKTR+SWPVFLEPPAD 
Sbjct: 241 FKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADT 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
            VGP P+LVD+ENP KYK KK+ +Y+YCKLNK+PQ
Sbjct: 301 VVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335

[Close]

BLAST of Spg008507 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 4.6e-143
Identity = 236/337 (70.03%), Postives = 277/337 (82.20%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDF--SNPDEDSVIR 60
           MEV RVQ I+       ++P E++R E+EQP +TT++G  L VPVID   SN  E++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTK 120
            IA+ASR WG+FQ+VNH IP++AIA LQ VGKEFFEL  +EKEV AKP G + VEGYGTK
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LQKEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLS 180
           LQKE+ GKKGWVDHLFHIIWP SA+NY FWP  PP YR ANEEY   L+GVVDKLF++LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGL 240
           LG+GLE HELK+ +GGD+L+Y+LKINYYPPCPRPDLALGV AHTDMS ITILVPNEVQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPA 300
           Q  +D HWYDV YIPNAL+IHIGDQIEI+SNGKYKSV HRTTVNKDKTR+SWPVFLEPP 
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 DFEVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           +   GP  KL+ +ENPAK+KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

[Close]

BLAST of Spg008507 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.5e-181
Identity = 301/335 (89.85%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGSLP EFVRP+TEQPGITTVKGQ LAVPVIDF NPDEDSVI+AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDG+KGWVDHLFHIIWPPSAIN+KFWP+ PPSYRAANEEYRKHL GVVDKLF+SLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Spg008507 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.8e-179
Identity = 298/335 (88.96%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQDIAS+ LFSGS+P EFVRP+ EQPGITTVKGQ LAVPVIDF NPDEDSVI+AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DASR+WGMFQI+NH+IPNEAIAHLQAVGK FFEL PEEKEVYAKP  SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KEVDGKKGWVDHLFHIIWPPSAIN+KFWP+ PPSYR ANEEYRKHL GVVDKLF+SLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELK+ AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMSTITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV KD+ RISWPVFLEPP +F
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP KYKTKKYGEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Spg008507 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.0e-178
Identity = 292/335 (87.16%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           M+VGRVQ++AS++LFSG+LP EFVRPETEQPGITTVKGQ LAVPVIDF+NPD+DSV+ AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DAS +WGMFQI+NH+IPNEAI HLQAVGKEFFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+INY FWP+ PPSYR ANEEYRK+L GVV+KLF+SLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKER GGDELVYLLKINYYPPCPRPDLALGVPAHTDMS +TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNK+KTRISWPVFLEPP +F
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
            VGPHPKLVDEENPAKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Spg008507 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.0e-178
Identity = 292/335 (87.16%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           M+VGRVQ++AS++LFSG+LP EFVRPETEQPGITTVKGQ LAVPVIDF+NPD+DSV+ AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DAS +WGMFQI+NH+IPNEAI HLQAVGKEFFEL PEEKE+YAKP  SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+INY FWP+ PPSYR ANEEYRK+L GVV+KLF+SLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKER GGDELVYLLKINYYPPCPRPDLALGVPAHTDMS +TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNK+KTRISWPVFLEPP +F
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
            VGPHPKLVDEENPAKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Spg008507 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.7e-177
Identity = 291/335 (86.87%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEVGRVQ++AS++LFSG+LP +FVRP+TEQPGITTVKGQ LAVPVIDF+NPD+DSV+ AI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
            DAS +WGMFQI+NH+IPNEAI HLQAVGKEFF+L PE KE+YAKP  SKSVEGYGT LQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+INY FWP+ PPSYRAANEEYRKHL GVV+KLF+SLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGLEGHELKER GGDELVYLLKINYYPPCPRPDLALGVPAHTDMS ITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNK+KTRISWPVFLEPP +F
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIPQ 336
           EVGPHPKLVDE+ PA+YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Spg008507 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 453.4 bits (1165), Expect = 1.6e-127
Identity = 206/334 (61.68%), Postives = 258/334 (77.25%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEV RVQDI+S+ L + ++P EF+R E EQP ITT +G T A+PV+D S+PDE+SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
             AS  WG+FQ+VNH IP E I  LQ VG++FFEL   EKE  AKP  SK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           K+ +GKK WVDHLFH IWPPS +NY+FWP+ PP YR  NEEY  H++ + + L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGL+   LKE  GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+VLHRTTV+K+KTR+SWPVFLEPP + 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIP 335
            VGP P+L  ++NP K+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Spg008507 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 453.4 bits (1165), Expect = 1.6e-127
Identity = 206/334 (61.68%), Postives = 258/334 (77.25%), Query Frame = 0


Query: 1   MEVGRVQDIASAVLFSGSLPPEFVRPETEQPGITTVKGQTLAVPVIDFSNPDEDSVIRAI 60
           MEV RVQDI+S+ L + ++P EF+R E EQP ITT +G T A+PV+D S+PDE+SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  ADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEEKEVYAKPAGSKSVEGYGTKLQ 120
             AS  WG+FQ+VNH IP E I  LQ VG++FFEL   EKE  AKP  SK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAANEEYRKHLQGVVDKLFKSLSLG 180
           K+ +GKK WVDHLFH IWPPS +NY+FWP+ PP YR  NEEY  H++ + + L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSTITILVPNEVQGLQA 240
           LGL+   LKE  GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKDKTRISWPVFLEPPADF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+VLHRTTV+K+KTR+SWPVFLEPP + 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPAKYKTKKYGEYAYCKLNKIP 335
            VGP P+L  ++NP K+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Spg008507 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 342.0 bits (876), Expect = 5.3e-94
Identity = 157/295 (53.22%), Postives = 203/295 (68.81%), Query Frame = 0


Query: 40  TLAVPVIDFSNPDEDSVIRAIADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFFELSPEE 99
           +L +PVID SNPDE+ V  A+  AS+ WG+FQ+VNH IP E I  L  VG EFFEL   E
Sbjct: 11  SLDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETE 70

Query: 100 KEVYAKPAGSKSVEGYGTKLQKEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPPSYRAAN 159
           KE  AKP  S  +EGY TK QK+++G+  WVDHLFH IWPPS +N+KFWP+ PP Y   N
Sbjct: 71  KEAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVN 130

Query: 160 EEYRKHLQGVVDKLFKSLSLGLGLEGHELKERAGGDELVYLLKINYYPPCPRPDLALGVP 219
           EEY  H++ + +K+ + LS GLGL    LKE  GG+ + YL+KINYYPPCP P+L +G P
Sbjct: 131 EEYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAP 190

Query: 220 AHTDMSTITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRT 279
            HTD++ IT+LV NE  GLQAF+D  W D  Y  + +++ IGDQ   +SNGKYKSV HR 
Sbjct: 191 DHTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRA 250

Query: 280 TVNKDKTRISWPVFLEPPADFEVGPHPKLV-DEENPAKYKTKKYGEYAYCKLNKI 334
            ++K+KTRISWPVF+E   D   GP P+L+  +EN  K+K   Y +Y + KL K+
Sbjct: 251 KMDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

[Close]

BLAST of Spg008507 vs. TAIR 10
Match: AT5G63600.1 (flavonol synthase 5 )

HSP 1 Score: 298.9 bits (764), Expect = 5.1e-81
Identity = 138/292 (47.26%), Postives = 190/292 (65.07%), Query Frame = 0


Query: 34  TTVKGQTLAVPVIDFSNPDEDSVIRAIADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFF 93
           +T+ G  + VPV+D S  DED ++R +  AS  WG+FQ+VNH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  ELSPEEKEVYAKPAGSKSVEGYGTKLQKEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPP 153
           EL   EKE  AK    +  EGY    +  + G   W +HLFH + PPS INYK+WP+ PP
Sbjct: 84  ELPDAEKETVAK---EEDFEGY---KKNYLGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRAANEEYRKHLQGVVDKLFKSLSLGLGLEGHELKERAGGDELVYLLKINYYPPCPRPD 213
            YR   EEY KH++ + +K+   LS GLGL+     +  GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSTITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYK 273
           L +G  AH+DM  I +L+PNEV GLQAF+D  W D++YI +A+V+ IGDQ+  ++NG+ K
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMRMTNGRLK 263

Query: 274 SVLHRTTVNKDKTRISWPVFLEPPADFEVGPHPKLVDEENPAKYKTKKYGEY 326
           +VLHR   +KDK RISWPVF+ P AD  VGP P+   +ENP K++T  Y +Y
Sbjct: 264 NVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 309

[Close]

BLAST of Spg008507 vs. TAIR 10
Match: AT5G63600.2 (flavonol synthase 5 )

HSP 1 Score: 296.6 bits (758), Expect = 2.5e-80
Identity = 138/293 (47.10%), Postives = 191/293 (65.19%), Query Frame = 0


Query: 34  TTVKGQTLAVPVIDFSNPDEDSVIRAIADASRHWGMFQIVNHEIPNEAIAHLQAVGKEFF 93
           +T+ G  + VPV+D S  DED ++R +  AS  WG+FQ+VNH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  ELSPEEKEVYAKPAGSKSVEGYGTKLQKEVDGKKGWVDHLFHIIWPPSAINYKFWPRTPP 153
           EL   EKE  AK    +  EGY    +  + G   W +HLFH + PPS INYK+WP+ PP
Sbjct: 84  ELPDAEKETVAK---EEDFEGY---KKNYLGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRAANEEYRKHLQGVVDKLFKSLSLGLGLEGHELKERAGGDELVYLLKINYYPPCPRPD 213
            YR   EEY KH++ + +K+   LS GLGL+     +  GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSTITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEI-LSNGKY 273
           L +G  AH+DM  I +L+PNEV GLQAF+D  W D++YI +A+V+ IGDQ+ + ++NG+ 
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMVWMTNGRL 263

Query: 274 KSVLHRTTVNKDKTRISWPVFLEPPADFEVGPHPKLVDEENPAKYKTKKYGEY 326
           K+VLHR   +KDK RISWPVF+ P AD  VGP P+   +ENP K++T  Y +Y
Sbjct: 264 KNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 310

The following BLAST results are available for this feature:

BLAST of Spg008507 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578981.1	3.1e-181	90.45	Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp...	[more]
XP_023551148.1	3.1e-181	90.45	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7016505.1	6.9e-181	90.15	Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp...	[more]
XP_022938382.1	1.5e-180	89.85	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata]	[more]
XP_022993310.1	3.8e-179	88.96	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima]	[more]

BLAST of Spg008507 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q07512	5.6e-149	72.32	Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ...	[more]
Q9M547	4.4e-146	71.64	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
Q9XHG2	6.4e-145	73.99	Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2...	[more]
Q9ZWQ9	3.2e-144	69.85	Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ...	[more]
Q7XZQ6	4.6e-143	70.03	Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS...	[more]

BLAST of Spg008507 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FDW3	7.5e-181	89.85	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JY72	1.8e-179	88.96	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5D3CHN5	2.0e-178	87.16	Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BCS7	2.0e-178	87.16	flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863...	[more]
A0A0A0K885	1.7e-177	86.87	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]

BLAST of Spg008507 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08640.1	1.6e-127	61.68	flavonol synthase 1	[more]
AT5G08640.2	1.6e-127	61.68	flavonol synthase 1	[more]
AT5G63590.1	5.3e-94	53.22	flavonol synthase 3	[more]
AT5G63600.1	5.1e-81	47.26	flavonol synthase 5	[more]
AT5G63600.2	2.5e-80	47.10	flavonol synthase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 43..140 e-value: 4.4E-22 score: 79.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..333 e-value: 1.8E-96 score: 325.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 201..296 e-value: 2.1E-25 score: 89.1
None	No IPR available	PANTHER	PTHR47990:SF36	OS02G0767300 PROTEIN	coord: 10..332
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..332
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 17..333
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 196..296 score: 14.804257

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008507.1	Spg008507.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051555	flavonol biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0045431	flavonol synthase activity
molecular_function	GO:0046872	metal ion binding