Spg008459 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg008459 vs. NCBI nr
Match: XP_038884306.1 (LOW QUALITY PROTEIN: putative wall-associated receptor kinase-like 16 [Benincasa hispida])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.4e-306
Identity = 539/747 (72.16%), Postives = 616/747 (82.46%), Query Frame = 0

Query: 1   MVVKIAILA--LAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKT-YDP 60
           MVV IAI +   AAAV SQA PGCE KC +LEIPYPFGM K CYL+ NF I CN+T Y P
Sbjct: 14  MVVNIAIFSAPAAAAVPSQALPGCESKCGDLEIPYPFGMKKGCYLNINFSITCNETHYHP 73

Query: 61  PKPFLMGSNIEVTNISLYGELQILQYVARDCYSKDG-PSVNNRPYLRVPMFTISNTKNKF 120
           PKPFLM  NIEVTNISLYGEL IL Y+ARDCY KDG P +NNRP L VPMF ISNTKNKF
Sbjct: 74  PKPFLMNGNIEVTNISLYGELHILNYIARDCYRKDGLPGINNRPRLTVPMFAISNTKNKF 133

Query: 121 TVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLT-RDGSCSGNGCCQLEIPKGLKYLDY 180
           TV+GCDTYAY+YG+L GESYTSGC+  C   + +T  DGSCSGNGCCQLEIPKGLK L  
Sbjct: 134 TVVGCDTYAYIYGDLPGESYTSGCMALCTAESDVTIEDGSCSGNGCCQLEIPKGLKLLRL 193

Query: 181 EVKSFNNHTGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTAN 240
           +V+SF+NHT V +FNPCG+AFV++Q+ F+FS  Y +   +DR+PLVLDWGIKNDTC   N
Sbjct: 194 DVRSFDNHTYVHSFNPCGFAFVVQQNQFSFSKKYIQNITEDRLPLVLDWGIKNDTCQPPN 253

Query: 241 NTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDH 300
               CLCGPNS RNSSLS+DGSEYYCQCLDGF GNPYL  GCQ+INECE     C+ +  
Sbjct: 254 QQRQCLCGPNSXRNSSLSLDGSEYYCQCLDGFHGNPYLPXGCQDINECEDGSDPCVPQAK 313

Query: 301 CKDTNGSYTCYCPDGF-GDGTLNGTRCTSKSLSP--VRIIVGVGVGFTVLLIGSTWMHLV 360
           C++  G+YTCYCP+ + GDG   G  C  K  +   ++II+G GVGF VLLIG +W++L 
Sbjct: 314 CENKAGNYTCYCPEKYEGDGKREGAGCKRKYSNSMLIQIIIGTGVGFVVLLIGISWLYLG 373

Query: 361 YKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKG 420
           YKKWKFIQ+K++FFKNNGG +LQQHLSQ QS  D+V+IF+QEELEKATNKF+ES VVGKG
Sbjct: 374 YKKWKFIQQKEKFFKNNGGTMLQQHLSQWQSPADTVKIFSQEELEKATNKFNESTVVGKG 433

Query: 421 GYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVP 480
           GYGTV+KG+L DGSVVAIKKS+LVDQSQTSQFINEVIVLS++NHRNVVKLLGCCLET+VP
Sbjct: 434 GYGTVHKGVLDDGSVVAIKKSQLVDQSQTSQFINEVIVLSQVNHRNVVKLLGCCLETQVP 493

Query: 481 LLVYEFITNGTLFDHIHDKTKHAS--LSWEARLKIASETAGVLSYLHSSASTPIIHRDIK 540
           LLVYEFI NGTLFDHIHD+TK+ S  LSWEARL+IASETAGV+SYLHSSASTPIIHRDIK
Sbjct: 494 LLVYEFIPNGTLFDHIHDQTKYYSNHLSWEARLRIASETAGVISYLHSSASTPIIHRDIK 553

Query: 541 STNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF 600
           S NILLDHN TAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF
Sbjct: 554 SANILLDHNLTAKVSDFGASKLVPMDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF 613

Query: 601 GIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEE-NFKQIKEVA 660
           GIVLLELITGK AVRFDGPE ERNLAMY   AMKEDR+ ++V+K M  +E    QIKEV 
Sbjct: 614 GIVLLELITGKKAVRFDGPEIERNLAMYVFRAMKEDRLVEIVDKGMVMDEGKLNQIKEVV 673

Query: 661 KLAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNS-NS 720
           K+AKEC++VKGEERP+MKEVAMELEGLR MQ++HSW N NLSN EEM  LLD   NS + 
Sbjct: 674 KVAKECVRVKGEERPSMKEVAMELEGLRVMQVQHSWINNNLSNSEEMISLLDETPNSTDQ 733

Query: 721 FVVGDSMNTMDYSIKAQIL-SRMPDGR 733
           F+V +S+N +D SIK  IL + +PD R
Sbjct: 734 FLVSNSINFVDNSIKIDILGTHIPDAR 760

BLAST of Spg008459 vs. NCBI nr
Match: XP_038886240.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.5e-292
Identity = 517/736 (70.24%), Postives = 595/736 (80.84%), Query Frame = 0

Query: 9   ALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKT-YDPPKPFLMGSNI 68
           ++A A A+ A   CE +C NLEIPYPFGM K CYL+ENF + CNKT Y PPK FL GSNI
Sbjct: 19  SVALATAAAASQDCERQCGNLEIPYPFGMKKGCYLNENFLVTCNKTHYHPPKAFLRGSNI 78

Query: 69  EVTNIS-LYGELQILQYVARDCYSKDG-PSVNN-RPYLRVPMFTISNTKNKFTVIGCDTY 128
           EVT+IS L  EL IL YVARDCY+KDG P ++  RP+L V MF+ISNTKNK T++GCDTY
Sbjct: 79  EVTHISILPSELHILNYVARDCYTKDGRPDISRARPFLVVDMFSISNTKNKLTIVGCDTY 138

Query: 129 AYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEVKSFNNHT 188
            YL+GE+ GE Y+SGC+  CGN+++  +DGSCSG+GCCQLEIPKGLK +  +V+SFNNHT
Sbjct: 139 GYLHGEIQGEIYSSGCMALCGNSSRTIKDGSCSGSGCCQLEIPKGLKEIQLDVRSFNNHT 198

Query: 189 GVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANNTDNCLCGP 248
            V + NPCGYAFVI+Q+ FTFS +Y + F + +VPLVLDWGIKNDTC   N+ D CLCGP
Sbjct: 199 RVHSLNPCGYAFVIQQNRFTFSKNYIKNFTESKVPLVLDWGIKNDTCQPPNHKDKCLCGP 258

Query: 249 NSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHCKDTNGSYT 308
           NS++NSSLS+DGSEYYCQCLDGF GNPYL  GCQ+I+EC+   H C  K  C +T G+YT
Sbjct: 259 NSQKNSSLSLDGSEYYCQCLDGFHGNPYLSQGCQDIDECQDGSHECKFKHQCVNTPGNYT 318

Query: 309 CYCPDGF-GDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYKKWKFIQRKD 368
           C CP  + GDG   G  CT  +   + II+G+ VG  VL I S W++L YKKW+FIQ+K 
Sbjct: 319 CNCPKNYKGDGRRGGEGCTQNTTFFILIIIGIVVGLLVLGISSMWLYLAYKKWRFIQQKA 378

Query: 369 RFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGYGTVYKGLLA 428
           +FF  NGG +LQ+H+SQ QSS+D +RIFT+EELEKATN FDES VVGKGGYGTVYKG+L 
Sbjct: 379 KFFNKNGGLVLQEHISQWQSSSDMLRIFTREELEKATNNFDESVVVGKGGYGTVYKGVLM 438

Query: 429 DGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLLVYEFITNGT 488
           DGS++AIKKSKLVDQSQT QFINEVI+LS+INHRNVVKLLGCCLET VPLLVYEFITNGT
Sbjct: 439 DGSIIAIKKSKLVDQSQTKQFINEVIILSQINHRNVVKLLGCCLETRVPLLVYEFITNGT 498

Query: 489 LFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDHNYTAK 548
           LF+HIH K  H+ LSW+ RLKIAS+TAGVLSYLHSSASTPIIHRDIKSTNILLDHNYTAK
Sbjct: 499 LFEHIHRKNNHSFLSWKTRLKIASQTAGVLSYLHSSASTPIIHRDIKSTNILLDHNYTAK 558

Query: 549 VSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKMA 608
           VSDFGASKLVPLDQTQ+STMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGK A
Sbjct: 559 VSDFGASKLVPLDQTQISTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGKKA 618

Query: 609 VRFDGPEAERNLAMYALCAMKEDRVEDVVEKR-MATEEN-FKQIKEVAKLAKECLKVKGE 668
           V F GPE ERNLAMY LCA+KEDRVE++VE   M TEE  F QIKEV KLAKECL+VKGE
Sbjct: 619 VTFTGPELERNLAMYVLCAIKEDRVEEIVENEIMETEEGVFWQIKEVTKLAKECLRVKGE 678

Query: 669 ERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVVG-DSM--NTM 728
           ERPTMKEVAMEL+ LR MQ+EH     NLS         D AS+S S  VG D+M  N M
Sbjct: 679 ERPTMKEVAMELDRLRVMQVEH----LNLS---------DEASDSTSINVGCDNMNNNAM 738

Query: 729 DYSIKAQILSRMPDGR 733
           D+SIKAQILSR+P GR
Sbjct: 739 DHSIKAQILSRIPLGR 741

BLAST of Spg008459 vs. NCBI nr
Match: XP_008441595.1 (PREDICTED: wall-associated receptor kinase 2-like [Cucumis melo] >TYK22298.1 wall-associated receptor kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 988.8 bits (2555), Expect = 2.5e-284
Identity = 506/745 (67.92%), Postives = 595/745 (79.87%), Query Frame = 0

Query: 1   MVVKIAILALAAAVAS---QAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKTYDP 60
           +VV IAI   A A +S   Q K  C+ KC NLEIPYPFG++++CYL+ NF IEC    D 
Sbjct: 16  IVVNIAIFLAATASSSVPPQVKFKCKSKCGNLEIPYPFGITEKCYLNINFSIECRPQEDR 75

Query: 61  PKPFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFT 120
             PFLM SNI+VTNISL+GE+ +L YVAR C   DG   +N+PY+ VPMFTISNTKNKFT
Sbjct: 76  EIPFLMNSNIKVTNISLHGEIHVLNYVARACSKSDGSLYSNKPYITVPMFTISNTKNKFT 135

Query: 121 VIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEV 180
           VIGCD+YAY++G++DGESY SGC+  CGN+T+  +DG CSG+GCCQLEIP+GLK +D  V
Sbjct: 136 VIGCDSYAYIFGQVDGESYRSGCMALCGNSTESIKDGPCSGSGCCQLEIPEGLKRIDLVV 195

Query: 181 KSFNNHTGV-LNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANN 240
            SFNN+T V  + NPCGYAFVIE+++F F   Y   + +  VPLVLDW IK+DTC     
Sbjct: 196 GSFNNYTTVNESINPCGYAFVIEENSFEFKKDYLTNYTEYEVPLVLDWRIKSDTC----P 255

Query: 241 TDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHC 300
           TD CLCGP S+    + ++GS YYCQC DGF GNPYL  GCQ+INECE   H C+    C
Sbjct: 256 TDKCLCGPKSQ----MIMNGSGYYCQCQDGFHGNPYLDQGCQDINECEEGSHLCVPDASC 315

Query: 301 KDT-NGSYTCYCPDGF-GDGTLNGTRCTSK--SLSPVRIIVGVGVGFTVLLIGSTWMHLV 360
           ++   G+YTC+CP+ + GDG   GT C  K  +   ++I +G GVGFTV LI  +W++L 
Sbjct: 316 ENAPAGNYTCHCPEKYEGDGRHGGTGCRQKYSNSKLIQIAIGTGVGFTVFLIAISWLYLG 375

Query: 361 YKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKG 420
           YKKW+FIQRK+ FFK NGG +LQQHLSQ QS TD+VRIF+QEELEKATNKF+ES VVGKG
Sbjct: 376 YKKWRFIQRKEEFFKKNGGTMLQQHLSQWQSPTDTVRIFSQEELEKATNKFNESTVVGKG 435

Query: 421 GYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVP 480
           GYGTV+KG+LADGSV+AIKKS+LVDQSQTSQFINEVIVLS++NHRNVVKLLGCCLETEVP
Sbjct: 436 GYGTVHKGVLADGSVIAIKKSQLVDQSQTSQFINEVIVLSQVNHRNVVKLLGCCLETEVP 495

Query: 481 LLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKST 540
           LLVYEFITNGTLFDHIHD+T +  +SWEARL+IASETAGV+SYLHSSASTPIIHRDIKST
Sbjct: 496 LLVYEFITNGTLFDHIHDRTIN-HISWEARLRIASETAGVISYLHSSASTPIIHRDIKST 555

Query: 541 NILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGI 600
           NILLDHN TAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYL TSELTEKSDVYSFGI
Sbjct: 556 NILLDHNLTAKVSDFGASKLVPMDQTQLSTMVQGTLGYLDPEYLSTSELTEKSDVYSFGI 615

Query: 601 VLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEE-NFKQIKEVAKL 660
           VLLELITGK AV FDGPEAERNLA Y LCAMKEDR+ +VV+K+M  +E    QIKEV+K+
Sbjct: 616 VLLELITGKKAVSFDGPEAERNLAKYVLCAMKEDRLAEVVDKKMVMDEGKLNQIKEVSKI 675

Query: 661 AKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVV 720
           AKEC++V+GEERP MKEVAMELEGL+ MQ++HSW N NLSN EEM  LLD  SNS  F++
Sbjct: 676 AKECVRVRGEERPNMKEVAMELEGLKVMQVKHSWINNNLSNSEEMISLLDETSNSTQFLL 735

Query: 721 GDSMN-TMDYSIKAQIL-SRMPDGR 733
             SMN T D SI   IL + +PD R
Sbjct: 736 SSSMNPTNDNSITTHILTTHIPDAR 751

BLAST of Spg008459 vs. NCBI nr
Match: XP_023512416.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 988.4 bits (2554), Expect = 3.3e-284
Identity = 507/740 (68.51%), Postives = 596/740 (80.54%), Query Frame = 0

Query: 1   MVVKIAILALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKT-YDPPK 60
           M+V I IL+ +   ASQA  GC D+C +L+IPYPFG  + CYL++NF I CN T +DPP+
Sbjct: 12  MIVNILILS-SPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHFDPPR 71

Query: 61  PFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNR---PYLRVPMFTISNTKNKF 120
           PFL   NI+VTNIS+ GELQIL + A+DCY K+  SV+ R     L +  FT+S+TKNKF
Sbjct: 72  PFLRTGNIQVTNISISGELQILHFGAKDCYPKNN-SVDARRTSATLNLSTFTVSSTKNKF 131

Query: 121 TVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYE 180
           TVIGCDTYA+L G+++G+SY + C+  C N T + RDG+CSGNGCCQL+IP GLK L Y 
Sbjct: 132 TVIGCDTYAFLSGQIEGQSYRTACVALCDNITTV-RDGACSGNGCCQLDIPSGLKGLRYR 191

Query: 181 VKSFNNHTGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANN 240
           V+SF+NHT VL+FNPCGYAFV E+D F FS++Y R+FPQ +VP+VLDWGI N TC TAN 
Sbjct: 192 VRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDWGISNTTCSTANK 251

Query: 241 TDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINEC-EGKFHNCLSKDH 300
             NC+CGPNS   + L  DGSEY C CLDGF+GNPYL  GCQ+I+EC + + ++C  K  
Sbjct: 252 KSNCVCGPNSMMVNPLP-DGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDC--KFE 311

Query: 301 CKDTNGSYTCYCPDGF-GDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYK 360
           C +T G+YTC CP+GF GDG   G  CT  S S V++I+GV VGFTVL+IGSTW++L Y+
Sbjct: 312 CVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYR 371

Query: 361 KWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGY 420
           KWK I+ K++FF+ NGG +LQ+HLSQ +SSTD V IFTQEEL+KATNK+DESAV+GKGGY
Sbjct: 372 KWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDESAVIGKGGY 431

Query: 421 GTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLL 480
           GTVYKG+L DGSVVAIKKSKLVDQSQTSQFINEVIVLS+INHRNVVKLLGCCLET+VPLL
Sbjct: 432 GTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLETQVPLL 491

Query: 481 VYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNI 540
           VYEF+TNGTLFDHIHD TKH  LSWEARL+IASETAGV+SYLHSSASTPIIHRDIK+TNI
Sbjct: 492 VYEFVTNGTLFDHIHDTTKHVPLSWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNI 551

Query: 541 LLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL 600
           LLD NY AKVSDFGASKLV LDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL
Sbjct: 552 LLDDNYNAKVSDFGASKLVTLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL 611

Query: 601 LELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKE 660
           LELITGK AV F+GPEAERNLAMY LCAMKEDR+ +VVEK MA E  F QIKEV K+A++
Sbjct: 612 LELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFGQIKEVGKVARK 671

Query: 661 CLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVVGDS 720
           CL++ GEERP+MKEVAMELEGLR M +EH W N       E   + DGA  SN FVV  S
Sbjct: 672 CLRISGEERPSMKEVAMELEGLRVM-VEHEWVN-------EENLVADGA--SNMFVVSGS 731

Query: 721 MNTMDYSIKAQILSRMPDGR 733
            N +D S+K QIL  + DGR
Sbjct: 732 TNVVDDSMKVQILPLIHDGR 735

BLAST of Spg008459 vs. NCBI nr
Match: XP_022989770.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 987.3 bits (2551), Expect = 7.3e-284
Identity = 502/740 (67.84%), Postives = 596/740 (80.54%), Query Frame = 0

Query: 1   MVVKIAILALAA-AVASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKT-YDPP 60
           M++ I IL+ +  AVASQA  GC+D+C +L+IPYPFG  + CYL++NF I CN T +DPP
Sbjct: 12  MIINILILSPSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHFDPP 71

Query: 61  KPFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNR--PYLRVPMFTISNTKNKF 120
           +PFL   NI+VTNIS+ GELQIL + A+DCY K+      R    L +  FT+S+TKNKF
Sbjct: 72  RPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSSTKNKF 131

Query: 121 TVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYE 180
           TVIGCDTYA+L G+++G+SY + C+  C N T + RDG+CSGNGCCQL+IP GLK L Y 
Sbjct: 132 TVIGCDTYAFLSGQIEGQSYRTACVALCDNITTV-RDGACSGNGCCQLDIPSGLKGLRYR 191

Query: 181 VKSFNNHTGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANN 240
           V+SF+NHT V +FNPCGYAFV E+D F FS++Y R FPQ +VP+VLDWGI N TC TANN
Sbjct: 192 VRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDWGISNTTCSTANN 251

Query: 241 TDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINEC-EGKFHNCLSKDH 300
             NC+CGPNS   + L  DGSEY C CLDGF+GNPYL  GCQ+I+EC + + ++C  K  
Sbjct: 252 KSNCICGPNSMMVNVLP-DGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDC--KFE 311

Query: 301 CKDTNGSYTCYCPDGF-GDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYK 360
           C +T G+YTC CP+GF GDG   G  CT  S S V++I+GV VGFTVL+IGSTW++L Y+
Sbjct: 312 CVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYR 371

Query: 361 KWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGY 420
           KWK I+ K++FF+ NGG +LQ+HLSQ +SSTD+V IFTQEEL+KATNK+DESAV+GKGGY
Sbjct: 372 KWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESAVIGKGGY 431

Query: 421 GTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLL 480
           GTVYKG+L DGSVVAIKKSKLVDQSQTSQFINEVIVLS+INHRNVVKLLGCCLET+VPLL
Sbjct: 432 GTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLETQVPLL 491

Query: 481 VYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNI 540
           VYEF+TNGTLFDHIHD TKH  LSW+ARL+IA ETAGV+SYLHSSASTPIIHRDIK+TNI
Sbjct: 492 VYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTPIIHRDIKTTNI 551

Query: 541 LLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL 600
           LLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL
Sbjct: 552 LLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL 611

Query: 601 LELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKE 660
           LELITGK AV F+GPEAERNLAMY LCAMKEDR+ +VVEK MA E  F+QIK+V K+A++
Sbjct: 612 LELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFEQIKDVGKVARK 671

Query: 661 CLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVVGDS 720
           CL++ GEERP+MKEV MELEGLR M  EH W       V+E   + DGA  SN FVV  S
Sbjct: 672 CLRISGEERPSMKEVVMELEGLRVM-AEHEW-------VDEENLVADGA--SNIFVVSGS 731

Query: 721 MNTMDYSIKAQILSRMPDGR 733
            N +D S+K Q+L  + DGR
Sbjct: 732 TNVVDDSMKVQVLPLIHDGR 737

BLAST of Spg008459 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.1e-170
Identity = 328/732 (44.81%), Postives = 466/732 (63.66%), Query Frame = 0

Query: 2   VVKIAILALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPK 61
           VV +  LA    V  Q +  C+ +C N+ + YPFG S  CY   DE+F + CN+     +
Sbjct: 9   VVAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNE-----Q 68

Query: 62  PFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNN-RPYLRVPMFTISNTKNKFTV 121
             L   N+ V N+SL G+L++    +R CY   G   +       +  FT+S   N+FTV
Sbjct: 69  EKLFFGNMPVINMSLSGQLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTV 128

Query: 122 IGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEVK 181
           +GC++YA+L      E Y++GCI  C + T  T++GSCSG GCCQ+ +P+G  ++  +  
Sbjct: 129 VGCNSYAFLRTS-GVEKYSTGCISICDSAT--TKNGSCSGEGCCQIPVPRGYSFVRVKPH 188

Query: 182 SFNNHTGVLNFNPCGYAFVIEQDNFTFSS----SYFREFPQDRVPLVLDWGIKNDTCLTA 241
           SF+NH  V  FNPC YAF++E   F F +    +  R       P+VLDW I + TC   
Sbjct: 189 SFHNHPTVHLFNPCTYAFLVEDGMFDFHALEDLNNLRNV--TTFPVVLDWSIGDKTCKQV 248

Query: 242 NNTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKD 301
                 +CG NS      S  G+ Y C+CL+GF+GNPYL  GCQ+INEC    HNC    
Sbjct: 249 EYRG--VCGGNS--TCFDSTGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHS 308

Query: 302 HCKDTNGSYTCYCPDGFGDGTLNGTRCTSKSLSP----VRIIVGVGVGFTVLLIGSTWMH 361
            C++T GS+ C CP G+   +LN   CT K         +I +G  +GF+V+++G + + 
Sbjct: 309 TCENTKGSFNCNCPSGYRKDSLNS--CTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQ 368

Query: 362 LVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVG 421
              K  K  + + +FF+ NGG +L Q +S    S   V+IFT++ +++ATN + ES ++G
Sbjct: 369 QKIKHRKNTELRQKFFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILG 428

Query: 422 KGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETE 481
           +GG GTVYKG+L D S+VAIKK++L ++SQ  QFINEV+VLS+INHRNVVK+LGCCLETE
Sbjct: 429 QGGQGTVYKGILPDNSIVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETE 488

Query: 482 VPLLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIK 541
           VPLLVYEFI +GTLFDH+H     +SL+WE RL+IA+E AG L+YLHSSAS PIIHRDIK
Sbjct: 489 VPLLVYEFINSGTLFDHLHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIK 548

Query: 542 STNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF 601
           + NILLD N TAKV+DFGAS+L+P+D+ QL+T+VQGTLGYLDPEY  T  L EKSDVYSF
Sbjct: 549 TANILLDKNLTAKVADFGASRLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSF 608

Query: 602 GIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAK 661
           G+VL+EL++G+ A+ F+ P   +NL      A K +R  ++++ ++  E+N ++I+E A+
Sbjct: 609 GVVLMELLSGQKALCFERPHCPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAAR 668

Query: 662 LAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWAN--KNLSNVEEM--TYLLDGASNS 717
           +A EC ++ GEERP MKEVA ELE LR    ++ W++  +    +E +    +L     +
Sbjct: 669 IAAECTRLMGEERPRMKEVAAELEALRVKTTKYKWSDQYRETGEIEHLLGVQILSAQGET 723

BLAST of Spg008459 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.4e-165
Identity = 317/700 (45.29%), Postives = 453/700 (64.71%), Query Frame = 0

Query: 8   LALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPKPFLMGS 67
           LA    V +Q +  C+ +C ++ I YPFG+S  CY   D++F I C    +  KP ++ S
Sbjct: 15  LAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITC----EEDKPNVL-S 74

Query: 68  NIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFTVIGCDTYA 127
           NIEV N +  G+L+ L   +  CY +   +     + R+   + S   NKFT++GC+ +A
Sbjct: 75  NIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFS-PNNKFTLVGCNAWA 134

Query: 128 YLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLE--IPKGLKYLDYEVKSFNNH 187
            L      ++Y++GC+  C  +T    +  C+G GCC+ E  IP     ++ +   F N 
Sbjct: 135 -LLSTFGIQNYSTGCMSLC--DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENM 194

Query: 188 TGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQD--RVPLVLDWGIKNDTCLTANNTDNCL 247
           T V +FNPC YAF +E   F FSS    +  ++  R P++LDW I N TC      +  +
Sbjct: 195 TSVEHFNPCSYAFFVEDGMFNFSSLEDLKDLRNVTRFPVLLDWSIGNQTCEQVVGRN--I 254

Query: 248 CGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHCKDTNG 307
           CG NS      S  G  Y C+CL GF GNPYL  GCQ+INEC  + HNC     C++T G
Sbjct: 255 CGGNS--TCFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLG 314

Query: 308 SYTCYCPDGFGDGT-----LNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYKKW 367
           S+ C CP G    T     ++  +   K L    +++G  +GF ++L+  +++    +  
Sbjct: 315 SFHCQCPSGSDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHR 374

Query: 368 KFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGYGT 427
           K  + + +FF+ NGG +L Q LS    S   V+IFT+E +++AT+ ++ES ++G+GG GT
Sbjct: 375 KNTELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGT 434

Query: 428 VYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLLVY 487
           VYKG+L D S+VAIKK++L D+SQ  QFINEV+VLS+INHRNVVKLLGCCLETEVPLLVY
Sbjct: 435 VYKGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVY 494

Query: 488 EFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILL 547
           EFI++GTLFDH+H     +SL+WE RL+IA E AG L+YLHS AS PIIHRD+K+ NILL
Sbjct: 495 EFISSGTLFDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILL 554

Query: 548 DHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLE 607
           D N TAKV+DFGAS+L+P+DQ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+E
Sbjct: 555 DENLTAKVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLME 614

Query: 608 LITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKECL 667
           L++G+ A+ F+ P++ ++L  Y + AMKE+R+ ++++ ++  E N ++I+E A++A EC 
Sbjct: 615 LLSGEKALCFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECT 674

Query: 668 KVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEM 695
           ++ GEERP+MKEVA ELE LR    +H W+++    VE +
Sbjct: 675 RIMGEERPSMKEVAAELEALRVKTTKHQWSDQYPKEVEHL 701

BLAST of Spg008459 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 581.3 bits (1497), Expect = 1.6e-164
Identity = 319/725 (44.00%), Postives = 452/725 (62.34%), Query Frame = 0

Query: 8   LALAAAVASQAKPG--CEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPKPFLM 67
           LA    V  Q +PG  C++KC N+ I YPFG+S  CY   +E+F I C +     +P ++
Sbjct: 16  LACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE----DRPHVL 75

Query: 68  GSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFTVIGCDT 127
            S+IEV N +  G+LQ+L   +  CY + G          +   ++S   NK T +GC+ 
Sbjct: 76  -SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLS-ANNKLTAVGCNA 135

Query: 128 YAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEVKS--FN 187
            + L      ++Y++ C+  C +  +   DG C+G GCC++++   L    +E  S    
Sbjct: 136 LS-LLDTFGMQNYSTACLSLCDSPPE--ADGECNGRGCCRVDVSAPLDSYTFETTSGRIK 195

Query: 188 NHTGVLNFNPCGYAFVIEQDNFTFSSS--YFREFPQDRVPLVLDWGIKNDTCLTANNTDN 247
           + T   +F+PC YAF++E D F FSS+          R P++LDW + N TC    +T  
Sbjct: 196 HMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGSTS- 255

Query: 248 CLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKF----HNCLSKDH 307
            +CG NS    S   +G  Y C+C +GF GNPYL  GCQ++NEC        HNC     
Sbjct: 256 -ICGGNSTCLDSTPRNG--YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKT 315

Query: 308 CKDTNGSYTCYCPDGFGDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYKK 367
           C++  G + C C  G+   T     C  K  +   I++   +GF V+L+G   +    K 
Sbjct: 316 CRNKVGGFYCKCQSGYRLDTTT-MSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKH 375

Query: 368 WKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGYG 427
            K  + +++FF+ NGG +L Q LS    S   V+IFT++ ++KATN + ES ++G+GG G
Sbjct: 376 LKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQG 435

Query: 428 TVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLLV 487
           TVYKG+L D S+VAIKK++L D SQ  QFINEV+VLS+INHRNVVKLLGCCLETEVPLLV
Sbjct: 436 TVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLV 495

Query: 488 YEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNIL 547
           YEFITNGTLFDH+H     +SL+WE RLKIA E AG L+YLHSSAS PIIHRDIK+ NIL
Sbjct: 496 YEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANIL 555

Query: 548 LDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLL 607
           LD N TAKV+DFGAS+L+P+D+ +L TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+
Sbjct: 556 LDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLM 615

Query: 608 ELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKEC 667
           EL++G+ A+ F  P++ ++L  Y   A KE+R+++++   +  E+N K+I+E A++A EC
Sbjct: 616 ELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAARIAAEC 675

Query: 668 LKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMT--YLLDGASNSNSFVVGD 717
            ++ GEERP MKEVA +LE LR  + +H W+++     E +   ++L     ++S +  D
Sbjct: 676 TRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSSIGYD 726

BLAST of Spg008459 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 575.9 bits (1483), Expect = 6.7e-163
Identity = 323/739 (43.71%), Postives = 462/739 (62.52%), Query Frame = 0

Query: 2   VVKIAILALAAAVASQAKP--GCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDP 61
           +V I  LA    V  Q +P   C+ KC N+ I YPFG+S  CY   D+NF    N T   
Sbjct: 9   LVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNF----NLTCVV 68

Query: 62  PKPFLMGSNIEVTNISLYGELQILQYVARDCY-SKDGPSVNNRPYLRVPMFTISNTKNKF 121
            +  L+   I+VTNIS  G + +L     +CY  K+  +     Y     F++S + NKF
Sbjct: 69  EEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTALGYQLGSSFSLS-SNNKF 128

Query: 122 TVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLE---IPKGLKYL 181
           T++GC+  + L      ++Y++GC+  C  N++   +G C+G GCC  E   +P      
Sbjct: 129 TLVGCNALS-LLSTFGKQNYSTGCLSLC--NSQPEANGRCNGVGCCTTEDFSVPFDSDTF 188

Query: 182 DYEVKSFNN---------HTGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQD--RVPLVL 241
            +      N         +T V  FNPC YAF++E   F F SS   +  ++  R P+ L
Sbjct: 189 QFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPVAL 248

Query: 242 DWGIKNDTCLTANNTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINE 301
           DW I N TC  A +T   +CG NS   +S + +G  Y C+C +G+ GNPY   GC++I+E
Sbjct: 249 DWSIGNQTCEQAGSTR--ICGKNSSCYNSTTRNG--YICKCNEGYDGNPYRSEGCKDIDE 308

Query: 302 CEGKFHNCLSKDHCKDTNGSYTCYCPDGFGDGTLNGT-RCTSKSLSPVRIIVGVGVGFTV 361
           C    HNC     C++ +G + C CP G+    LN +  CT       RI + + +G  V
Sbjct: 309 CISDTHNCSDPKTCRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIGVLV 368

Query: 362 LLIGSTWMHLVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATN 421
           LL+ +  +    K+ K+ + + +FF+ NGG +L Q LS    S    +IFT+E +++ATN
Sbjct: 369 LLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATN 428

Query: 422 KFDESAVVGKGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVK 481
            +DES ++G+GG GTVYKG+L D ++VAIKK++L D  Q  QFI+EV+VLS+INHRNVVK
Sbjct: 429 GYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVK 488

Query: 482 LLGCCLETEVPLLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSAS 541
           +LGCCLETEVPLLVYEFITNGTLFDH+H     +SL+WE RL+IA E AG L+YLHSSAS
Sbjct: 489 ILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLHSSAS 548

Query: 542 TPIIHRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSEL 601
            PIIHRDIK+ NILLD N TAKV+DFGASKL+P+D+ QL+TMVQGTLGYLDPEY  T  L
Sbjct: 549 IPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTTGLL 608

Query: 602 TEKSDVYSFGIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEEN 661
            EKSDVYSFG+VL+EL++G+ A+ F+ P+A ++L  Y + A +E+R+ ++++ ++  E+N
Sbjct: 609 NEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVLNEDN 668

Query: 662 FKQIKEVAKLAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMT--YL 717
            K+I+E A++A EC ++ GEERP MKEVA +LE LR  + +H W+++     E +   ++
Sbjct: 669 LKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHI 728

BLAST of Spg008459 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 5.6e-154
Identity = 307/704 (43.61%), Postives = 432/704 (61.36%), Query Frame = 0

Query: 2   VVKIAILALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPK 61
           +V I  L+    V  Q  P C +KC N+ + YPFG S  C+   D +F + C        
Sbjct: 8   LVAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC------VN 67

Query: 62  PFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPY-LRVPMFTISNTKNKFTV 121
             L    +EV  IS   +L++L   +  CY+  G       Y   +   T+S   N  T 
Sbjct: 68  ENLFYKGLEVVEISHSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSG-NNTITA 127

Query: 122 IGCDTYAYLYGELDGESYTS-GCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEV 181
           +GC++YA++    +G    S GCI  C +      +G C+G GCCQ  +P G  +L    
Sbjct: 128 LGCNSYAFV--SSNGTRRNSVGCISAC-DALSHEANGECNGEGCCQNPVPAGNNWLIVRS 187

Query: 182 KSFNNHTGV--LNFNPCGYAFVIEQDNFTFSSSYFREFPQDR---VPLVLDWGIKNDTCL 241
             F+N T V  ++   C YAF++E   F +++S    + Q+R    P+VLDW I+ +TC 
Sbjct: 188 YRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETC- 247

Query: 242 TANNTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEG----KFH 301
                    CG N   ++S S  G  Y C+C  GFQGNPYL  GCQ+INEC        H
Sbjct: 248 --GQVGEKKCGVNGICSNSAS--GIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKH 307

Query: 302 NCLSKDHCKDTNGSYTCYCPDGFGDGTLNGTRCTSKS----LSPVRIIVGVGVGFTVLLI 361
           NC     C++  G + C C   +   T   T C  K     +    I++G  +GF V+L+
Sbjct: 308 NCSGDSTCENKLGHFRCNCRSRYELNTTTNT-CKPKGNPEYVEWTTIVLGTTIGFLVILL 367

Query: 362 GSTWMHLVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFD 421
             + +    K  K  + + +FF+ NGG +L Q LS    S   V+IFT+E +++AT+ +D
Sbjct: 368 AISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYD 427

Query: 422 ESAVVGKGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLG 481
           E+ ++G+GG GTVYKG+L D S+VAIKK++L D SQ  QFINEV+VLS+INHRNVVKLLG
Sbjct: 428 ENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLG 487

Query: 482 CCLETEVPLLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPI 541
           CCLETEVPLLVYEFI++GTLFDH+H     +SL+WE RL++A E AG L+YLHSSAS PI
Sbjct: 488 CCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPI 547

Query: 542 IHRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 601
           IHRDIK+ NILLD N TAKV+DFGAS+L+P+D+  L+TMVQGTLGYLDPEY  T  L EK
Sbjct: 548 IHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEK 607

Query: 602 SDVYSFGIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQ 661
           SDVYSFG+VL+EL++G+ A+ F+ P+  +++  Y   A KE+R+ ++++ ++  E N ++
Sbjct: 608 SDVYSFGVVLMELLSGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQRE 667

Query: 662 IKEVAKLAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANK 687
           I++ A++A EC ++ GEERP MKEVA ELE LR  + +H W+++
Sbjct: 668 IQKAARIAVECTRLTGEERPGMKEVAAELEALRVTKTKHKWSDE 695

BLAST of Spg008459 vs. ExPASy TrEMBL
Match: A0A5D3DFR4 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3926G00050 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 1.2e-284
Identity = 506/745 (67.92%), Postives = 595/745 (79.87%), Query Frame = 0

Query: 1   MVVKIAILALAAAVAS---QAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKTYDP 60
           +VV IAI   A A +S   Q K  C+ KC NLEIPYPFG++++CYL+ NF IEC    D 
Sbjct: 16  IVVNIAIFLAATASSSVPPQVKFKCKSKCGNLEIPYPFGITEKCYLNINFSIECRPQEDR 75

Query: 61  PKPFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFT 120
             PFLM SNI+VTNISL+GE+ +L YVAR C   DG   +N+PY+ VPMFTISNTKNKFT
Sbjct: 76  EIPFLMNSNIKVTNISLHGEIHVLNYVARACSKSDGSLYSNKPYITVPMFTISNTKNKFT 135

Query: 121 VIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEV 180
           VIGCD+YAY++G++DGESY SGC+  CGN+T+  +DG CSG+GCCQLEIP+GLK +D  V
Sbjct: 136 VIGCDSYAYIFGQVDGESYRSGCMALCGNSTESIKDGPCSGSGCCQLEIPEGLKRIDLVV 195

Query: 181 KSFNNHTGV-LNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANN 240
            SFNN+T V  + NPCGYAFVIE+++F F   Y   + +  VPLVLDW IK+DTC     
Sbjct: 196 GSFNNYTTVNESINPCGYAFVIEENSFEFKKDYLTNYTEYEVPLVLDWRIKSDTC----P 255

Query: 241 TDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHC 300
           TD CLCGP S+    + ++GS YYCQC DGF GNPYL  GCQ+INECE   H C+    C
Sbjct: 256 TDKCLCGPKSQ----MIMNGSGYYCQCQDGFHGNPYLDQGCQDINECEEGSHLCVPDASC 315

Query: 301 KDT-NGSYTCYCPDGF-GDGTLNGTRCTSK--SLSPVRIIVGVGVGFTVLLIGSTWMHLV 360
           ++   G+YTC+CP+ + GDG   GT C  K  +   ++I +G GVGFTV LI  +W++L 
Sbjct: 316 ENAPAGNYTCHCPEKYEGDGRHGGTGCRQKYSNSKLIQIAIGTGVGFTVFLIAISWLYLG 375

Query: 361 YKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKG 420
           YKKW+FIQRK+ FFK NGG +LQQHLSQ QS TD+VRIF+QEELEKATNKF+ES VVGKG
Sbjct: 376 YKKWRFIQRKEEFFKKNGGTMLQQHLSQWQSPTDTVRIFSQEELEKATNKFNESTVVGKG 435

Query: 421 GYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVP 480
           GYGTV+KG+LADGSV+AIKKS+LVDQSQTSQFINEVIVLS++NHRNVVKLLGCCLETEVP
Sbjct: 436 GYGTVHKGVLADGSVIAIKKSQLVDQSQTSQFINEVIVLSQVNHRNVVKLLGCCLETEVP 495

Query: 481 LLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKST 540
           LLVYEFITNGTLFDHIHD+T +  +SWEARL+IASETAGV+SYLHSSASTPIIHRDIKST
Sbjct: 496 LLVYEFITNGTLFDHIHDRTIN-HISWEARLRIASETAGVISYLHSSASTPIIHRDIKST 555

Query: 541 NILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGI 600
           NILLDHN TAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYL TSELTEKSDVYSFGI
Sbjct: 556 NILLDHNLTAKVSDFGASKLVPMDQTQLSTMVQGTLGYLDPEYLSTSELTEKSDVYSFGI 615

Query: 601 VLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEE-NFKQIKEVAKL 660
           VLLELITGK AV FDGPEAERNLA Y LCAMKEDR+ +VV+K+M  +E    QIKEV+K+
Sbjct: 616 VLLELITGKKAVSFDGPEAERNLAKYVLCAMKEDRLAEVVDKKMVMDEGKLNQIKEVSKI 675

Query: 661 AKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVV 720
           AKEC++V+GEERP MKEVAMELEGL+ MQ++HSW N NLSN EEM  LLD  SNS  F++
Sbjct: 676 AKECVRVRGEERPNMKEVAMELEGLKVMQVKHSWINNNLSNSEEMISLLDETSNSTQFLL 735

Query: 721 GDSMN-TMDYSIKAQIL-SRMPDGR 733
             SMN T D SI   IL + +PD R
Sbjct: 736 SSSMNPTNDNSITTHILTTHIPDAR 751

BLAST of Spg008459 vs. ExPASy TrEMBL
Match: A0A1S3B4I0 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485674 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 1.2e-284
Identity = 506/745 (67.92%), Postives = 595/745 (79.87%), Query Frame = 0

Query: 1   MVVKIAILALAAAVAS---QAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKTYDP 60
           +VV IAI   A A +S   Q K  C+ KC NLEIPYPFG++++CYL+ NF IEC    D 
Sbjct: 16  IVVNIAIFLAATASSSVPPQVKFKCKSKCGNLEIPYPFGITEKCYLNINFSIECRPQEDR 75

Query: 61  PKPFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFT 120
             PFLM SNI+VTNISL+GE+ +L YVAR C   DG   +N+PY+ VPMFTISNTKNKFT
Sbjct: 76  EIPFLMNSNIKVTNISLHGEIHVLNYVARACSKSDGSLYSNKPYITVPMFTISNTKNKFT 135

Query: 121 VIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEV 180
           VIGCD+YAY++G++DGESY SGC+  CGN+T+  +DG CSG+GCCQLEIP+GLK +D  V
Sbjct: 136 VIGCDSYAYIFGQVDGESYRSGCMALCGNSTESIKDGPCSGSGCCQLEIPEGLKRIDLVV 195

Query: 181 KSFNNHTGV-LNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANN 240
            SFNN+T V  + NPCGYAFVIE+++F F   Y   + +  VPLVLDW IK+DTC     
Sbjct: 196 GSFNNYTTVNESINPCGYAFVIEENSFEFKKDYLTNYTEYEVPLVLDWRIKSDTC----P 255

Query: 241 TDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHC 300
           TD CLCGP S+    + ++GS YYCQC DGF GNPYL  GCQ+INECE   H C+    C
Sbjct: 256 TDKCLCGPKSQ----MIMNGSGYYCQCQDGFHGNPYLDQGCQDINECEEGSHLCVPDASC 315

Query: 301 KDT-NGSYTCYCPDGF-GDGTLNGTRCTSK--SLSPVRIIVGVGVGFTVLLIGSTWMHLV 360
           ++   G+YTC+CP+ + GDG   GT C  K  +   ++I +G GVGFTV LI  +W++L 
Sbjct: 316 ENAPAGNYTCHCPEKYEGDGRHGGTGCRQKYSNSKLIQIAIGTGVGFTVFLIAISWLYLG 375

Query: 361 YKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKG 420
           YKKW+FIQRK+ FFK NGG +LQQHLSQ QS TD+VRIF+QEELEKATNKF+ES VVGKG
Sbjct: 376 YKKWRFIQRKEEFFKKNGGTMLQQHLSQWQSPTDTVRIFSQEELEKATNKFNESTVVGKG 435

Query: 421 GYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVP 480
           GYGTV+KG+LADGSV+AIKKS+LVDQSQTSQFINEVIVLS++NHRNVVKLLGCCLETEVP
Sbjct: 436 GYGTVHKGVLADGSVIAIKKSQLVDQSQTSQFINEVIVLSQVNHRNVVKLLGCCLETEVP 495

Query: 481 LLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKST 540
           LLVYEFITNGTLFDHIHD+T +  +SWEARL+IASETAGV+SYLHSSASTPIIHRDIKST
Sbjct: 496 LLVYEFITNGTLFDHIHDRTIN-HISWEARLRIASETAGVISYLHSSASTPIIHRDIKST 555

Query: 541 NILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGI 600
           NILLDHN TAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYL TSELTEKSDVYSFGI
Sbjct: 556 NILLDHNLTAKVSDFGASKLVPMDQTQLSTMVQGTLGYLDPEYLSTSELTEKSDVYSFGI 615

Query: 601 VLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEE-NFKQIKEVAKL 660
           VLLELITGK AV FDGPEAERNLA Y LCAMKEDR+ +VV+K+M  +E    QIKEV+K+
Sbjct: 616 VLLELITGKKAVSFDGPEAERNLAKYVLCAMKEDRLAEVVDKKMVMDEGKLNQIKEVSKI 675

Query: 661 AKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVV 720
           AKEC++V+GEERP MKEVAMELEGL+ MQ++HSW N NLSN EEM  LLD  SNS  F++
Sbjct: 676 AKECVRVRGEERPNMKEVAMELEGLKVMQVKHSWINNNLSNSEEMISLLDETSNSTQFLL 735

Query: 721 GDSMN-TMDYSIKAQIL-SRMPDGR 733
             SMN T D SI   IL + +PD R
Sbjct: 736 SSSMNPTNDNSITTHILTTHIPDAR 751

BLAST of Spg008459 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 3.5e-284
Identity = 502/740 (67.84%), Postives = 596/740 (80.54%), Query Frame = 0

Query: 1   MVVKIAILALAA-AVASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKT-YDPP 60
           M++ I IL+ +  AVASQA  GC+D+C +L+IPYPFG  + CYL++NF I CN T +DPP
Sbjct: 12  MIINILILSPSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHFDPP 71

Query: 61  KPFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNR--PYLRVPMFTISNTKNKF 120
           +PFL   NI+VTNIS+ GELQIL + A+DCY K+      R    L +  FT+S+TKNKF
Sbjct: 72  RPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSSTKNKF 131

Query: 121 TVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYE 180
           TVIGCDTYA+L G+++G+SY + C+  C N T + RDG+CSGNGCCQL+IP GLK L Y 
Sbjct: 132 TVIGCDTYAFLSGQIEGQSYRTACVALCDNITTV-RDGACSGNGCCQLDIPSGLKGLRYR 191

Query: 181 VKSFNNHTGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANN 240
           V+SF+NHT V +FNPCGYAFV E+D F FS++Y R FPQ +VP+VLDWGI N TC TANN
Sbjct: 192 VRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDWGISNTTCSTANN 251

Query: 241 TDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINEC-EGKFHNCLSKDH 300
             NC+CGPNS   + L  DGSEY C CLDGF+GNPYL  GCQ+I+EC + + ++C  K  
Sbjct: 252 KSNCICGPNSMMVNVLP-DGSEYRCGCLDGFEGNPYLPRGCQDIDECRDERLNDC--KFE 311

Query: 301 CKDTNGSYTCYCPDGF-GDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYK 360
           C +T G+YTC CP+GF GDG   G  CT  S S V++I+GV VGFTVL+IGSTW++L Y+
Sbjct: 312 CVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIGSTWLYLGYR 371

Query: 361 KWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGY 420
           KWK I+ K++FF+ NGG +LQ+HLSQ +SSTD+V IFTQEEL+KATNK+DESAV+GKGGY
Sbjct: 372 KWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDESAVIGKGGY 431

Query: 421 GTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLL 480
           GTVYKG+L DGSVVAIKKSKLVDQSQTSQFINEVIVLS+INHRNVVKLLGCCLET+VPLL
Sbjct: 432 GTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGCCLETQVPLL 491

Query: 481 VYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNI 540
           VYEF+TNGTLFDHIHD TKH  LSW+ARL+IA ETAGV+SYLHSSASTPIIHRDIK+TNI
Sbjct: 492 VYEFVTNGTLFDHIHDTTKHVPLSWKARLRIALETAGVISYLHSSASTPIIHRDIKTTNI 551

Query: 541 LLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL 600
           LLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL
Sbjct: 552 LLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL 611

Query: 601 LELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKE 660
           LELITGK AV F+GPEAERNLAMY LCAMKEDR+ +VVEK MA E  F+QIK+V K+A++
Sbjct: 612 LELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFEQIKDVGKVARK 671

Query: 661 CLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGASNSNSFVVGDS 720
           CL++ GEERP+MKEV MELEGLR M  EH W       V+E   + DGA  SN FVV  S
Sbjct: 672 CLRISGEERPSMKEVVMELEGLRVM-AEHEW-------VDEENLVADGA--SNIFVVSGS 731

Query: 721 MNTMDYSIKAQILSRMPDGR 733
            N +D S+K Q+L  + DGR
Sbjct: 732 TNVVDDSMKVQVLPLIHDGR 737

BLAST of Spg008459 vs. ExPASy TrEMBL
Match: A0A0A0KDE6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G349850 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.8e-280
Identity = 504/752 (67.02%), Postives = 595/752 (79.12%), Query Frame = 0

Query: 1   MVVKIAILALAAA---VASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKT-YD 60
           +VV IAI + A +   V S AK GCE KC ++EIP+PFGMS  CYL+ NF I CN T + 
Sbjct: 16  IVVNIAIFSAAVSSSPVPSLAKLGCESKCGDVEIPFPFGMSDNCYLNINFSITCNHTHFT 75

Query: 61  PPKPFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPS-VNNRPYLRVPMFTISNTKNK 120
           P KPFLM SN+EVTNISL+GEL +L YVAR CYS+DG + + NRP +RVPMFTISNTKNK
Sbjct: 76  PAKPFLMNSNVEVTNISLHGELHVLNYVARACYSEDGSADIKNRPSIRVPMFTISNTKNK 135

Query: 121 FTVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDY 180
           FTVIGCDTYAY+ GELDGESY SGC+  CG   K  +DGSC  +GCCQLEIPKGL+ L  
Sbjct: 136 FTVIGCDTYAYISGELDGESYRSGCMALCGTFRKNIKDGSC-WSGCCQLEIPKGLQKLAL 195

Query: 181 EVKSFNNHT-----GVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDT 240
           EV SF+N+T       LN + CGYAFVIEQ+ F F  SY   + +++VPLVLDW IK++ 
Sbjct: 196 EVGSFHNYTEPENKSNLNLSQCGYAFVIEQNIFNFKKSYINNYTEEKVPLVLDWKIKHEN 255

Query: 241 CLTANNTDNCLCGPNSKRNSSLSVDGSEYY-CQCLDGFQGNPYL--GCQNINECEGKFHN 300
           C    +TD C CG  S++   +  DGS+YY C+C +G+ GNPYL  GCQ+ NEC+   H 
Sbjct: 256 C----STDKCKCGQKSEK---ILEDGSKYYRCKCPNGYHGNPYLDEGCQDTNECKLGTHQ 315

Query: 301 CLSKDHCKDT-NGSYTCYCPDGF-GDGTLNGTRCTSKSLSP--VRIIVGVGVGFTVLLIG 360
           C+S D C++   G+YTCYCP+ + GDG   GT C  K  +   ++I  G GVG TVLLI 
Sbjct: 316 CVSNDMCENAPEGTYTCYCPENYEGDGKEGGTGCRKKHSNSKFIKIATGTGVGITVLLIA 375

Query: 361 STWMHLVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDE 420
            +W++L YKKWKFIQRK+ FFK NGG +LQQHLSQ QS TD+VRIF+QEELEKATNKF+E
Sbjct: 376 ISWLYLGYKKWKFIQRKEEFFKKNGGTMLQQHLSQWQSPTDTVRIFSQEELEKATNKFNE 435

Query: 421 SAVVGKGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGC 480
           S VVGKGGYGTV+KG+L DGSV+AIKKS+L+DQSQTSQFINEVIVLS++NHRNVVKLLGC
Sbjct: 436 STVVGKGGYGTVHKGVLDDGSVIAIKKSQLLDQSQTSQFINEVIVLSQVNHRNVVKLLGC 495

Query: 481 CLETEVPLLVYEFITNGTLFDHIHDKTKHAS-LSWEARLKIASETAGVLSYLHSSASTPI 540
           CLET+VPLLVYEFITNGTLFDHIHD+TK+++ + WEARL+IASETAGV+SYLHSSASTP+
Sbjct: 496 CLETQVPLLVYEFITNGTLFDHIHDRTKYSNHIPWEARLRIASETAGVISYLHSSASTPV 555

Query: 541 IHRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 600
           IHRDIKSTNILLDHN+TAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYLL SELTEK
Sbjct: 556 IHRDIKSTNILLDHNFTAKVSDFGASKLVPMDQTQLSTMVQGTLGYLDPEYLLKSELTEK 615

Query: 601 SDVYSFGIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEE-NFK 660
           SDVYSFGIVLLELITGK AV FDGPEAERNLAMY LCAMKEDR+ +VV+K M  +E    
Sbjct: 616 SDVYSFGIVLLELITGKKAVCFDGPEAERNLAMYVLCAMKEDRLAEVVDKEMVMDEGKLN 675

Query: 661 QIKEVAKLAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMTYLLDGA 720
           QIKEV+K+AKEC++V+GEERP MKEVAMELEGL+ MQ++HSW   NLSN EEM  LL   
Sbjct: 676 QIKEVSKIAKECVRVRGEERPNMKEVAMELEGLKVMQVQHSWIKNNLSNSEEMISLLGET 735

Query: 721 SNSNSFVVGDSMNTMDYSIKAQIL-SRMPDGR 733
           SNS  F+V   MN+   SI   IL + +PD R
Sbjct: 736 SNSTQFLVSSRMNSTSNSITTDILTAHVPDAR 759

BLAST of Spg008459 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.8e-280
Identity = 503/735 (68.44%), Postives = 588/735 (80.00%), Query Frame = 0

Query: 6   AILALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYLDENFFIECNKTY-DPPKPFLMG 65
           A  A AAA ASQA PGCE+ C +++IPYPFGM + CYL+E FFI CNKT+ D PK FL G
Sbjct: 19  AAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFFISCNKTHNDSPKAFLQG 78

Query: 66  SNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFTVIGCDTY 125
            N+ VTNIS+ GEL IL + ARDCY  +     +  +L    F +S+ KNKFTVIGCDT+
Sbjct: 79  GNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTE-FAVSSAKNKFTVIGCDTF 138

Query: 126 AYLYGELDG-ESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEVKSFNNH 185
           +++ G + G + Y S C+  C + T + +DG+CSGNGCCQLEIPKGL  L+Y V SF+NH
Sbjct: 139 SFISGLVGGTQFYKSACVALCDDITTV-KDGACSGNGCCQLEIPKGLNNLNYTVSSFDNH 198

Query: 186 TGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQDRVPLVLDWGIKNDTCLTANNTDNCLCG 245
           T VL+FNPCGYAFVIE+D F FSS Y R FP +RVPLVLDW I N+TC    NT NC+CG
Sbjct: 199 TNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAISNNTC---ENTTNCICG 258

Query: 246 PNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHCKDTNGSY 305
           P+S++ + +  DGSEY CQC DGF+GNPYL  GCQ+++EC+   H+C  K  C +T G+Y
Sbjct: 259 PSSRKVNFVE-DGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDC--KFECVNTEGNY 318

Query: 306 TCYCPDGF-GDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYKKWKFIQRK 365
           TC CP+GF GDG   G  CT  S S V+IIVGV VGFTVLLIG TW +L Y+KWKF++ K
Sbjct: 319 TCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLK 378

Query: 366 DRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGYGTVYKGLL 425
           + FF+ NGG +LQQHLSQ Q+S D VRIFTQEELEKATNK+DESAVVGKGGYGTVYKG+L
Sbjct: 379 EEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVL 438

Query: 426 ADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLLVYEFITNG 485
            DG VVAIKKSKLVDQSQTSQFINEV+VLS+INHRNVVKLLGCCLET+VPLLVYEFITNG
Sbjct: 439 EDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNG 498

Query: 486 TLFDHIHDKTKH-ASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILLDHNYT 545
           TL+DHIHDK  H  SL WEARL+IASETAGV+SYLHSSASTPIIHRDIK+TNILLD NYT
Sbjct: 499 TLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYT 558

Query: 546 AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLELITGK 605
           AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVL+ELITGK
Sbjct: 559 AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGK 618

Query: 606 MAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKECLKVKGE 665
            AV F+GPEAERNLAMY LCAMKEDR+E+VVEK MA E   +QIKEVAK+AKECL+V+GE
Sbjct: 619 KAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGE 678

Query: 666 ERPTMKEVAMELEGLRQMQIEHSWAN-KNLSNVEEM-TYLLDGASNSNSFVVGDSMNTMD 725
           ERP+MKEVAMELEGLR + +E+ W N  NL N EEM  YLL+  + ++   +    N +D
Sbjct: 679 ERPSMKEVAMELEGLRVL-VENKWVNDNNLINAEEMVAYLLEDNNGASESTLLSMNNVVD 738

Query: 726 YSIKAQILSRMPDGR 733
            S+K QIL R+ DGR
Sbjct: 739 DSMKVQILPRIHDGR 744

BLAST of Spg008459 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 601.7 bits (1550), Expect = 8.0e-172
Identity = 328/732 (44.81%), Postives = 466/732 (63.66%), Query Frame = 0

Query: 2   VVKIAILALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPK 61
           VV +  LA    V  Q +  C+ +C N+ + YPFG S  CY   DE+F + CN+     +
Sbjct: 9   VVAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNE-----Q 68

Query: 62  PFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNN-RPYLRVPMFTISNTKNKFTV 121
             L   N+ V N+SL G+L++    +R CY   G   +       +  FT+S   N+FTV
Sbjct: 69  EKLFFGNMPVINMSLSGQLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTV 128

Query: 122 IGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEVK 181
           +GC++YA+L      E Y++GCI  C + T  T++GSCSG GCCQ+ +P+G  ++  +  
Sbjct: 129 VGCNSYAFLRTS-GVEKYSTGCISICDSAT--TKNGSCSGEGCCQIPVPRGYSFVRVKPH 188

Query: 182 SFNNHTGVLNFNPCGYAFVIEQDNFTFSS----SYFREFPQDRVPLVLDWGIKNDTCLTA 241
           SF+NH  V  FNPC YAF++E   F F +    +  R       P+VLDW I + TC   
Sbjct: 189 SFHNHPTVHLFNPCTYAFLVEDGMFDFHALEDLNNLRNV--TTFPVVLDWSIGDKTCKQV 248

Query: 242 NNTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKD 301
                 +CG NS      S  G+ Y C+CL+GF+GNPYL  GCQ+INEC    HNC    
Sbjct: 249 EYRG--VCGGNS--TCFDSTGGTGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHS 308

Query: 302 HCKDTNGSYTCYCPDGFGDGTLNGTRCTSKSLSP----VRIIVGVGVGFTVLLIGSTWMH 361
            C++T GS+ C CP G+   +LN   CT K         +I +G  +GF+V+++G + + 
Sbjct: 309 TCENTKGSFNCNCPSGYRKDSLNS--CTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQ 368

Query: 362 LVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVG 421
              K  K  + + +FF+ NGG +L Q +S    S   V+IFT++ +++ATN + ES ++G
Sbjct: 369 QKIKHRKNTELRQKFFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILG 428

Query: 422 KGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETE 481
           +GG GTVYKG+L D S+VAIKK++L ++SQ  QFINEV+VLS+INHRNVVK+LGCCLETE
Sbjct: 429 QGGQGTVYKGILPDNSIVAIKKARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETE 488

Query: 482 VPLLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIK 541
           VPLLVYEFI +GTLFDH+H     +SL+WE RL+IA+E AG L+YLHSSAS PIIHRDIK
Sbjct: 489 VPLLVYEFINSGTLFDHLHGSLYDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIK 548

Query: 542 STNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF 601
           + NILLD N TAKV+DFGAS+L+P+D+ QL+T+VQGTLGYLDPEY  T  L EKSDVYSF
Sbjct: 549 TANILLDKNLTAKVADFGASRLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSF 608

Query: 602 GIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAK 661
           G+VL+EL++G+ A+ F+ P   +NL      A K +R  ++++ ++  E+N ++I+E A+
Sbjct: 609 GVVLMELLSGQKALCFERPHCPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAAR 668

Query: 662 LAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWAN--KNLSNVEEM--TYLLDGASNS 717
           +A EC ++ GEERP MKEVA ELE LR    ++ W++  +    +E +    +L     +
Sbjct: 669 IAAECTRLMGEERPRMKEVAAELEALRVKTTKYKWSDQYRETGEIEHLLGVQILSAQGET 723

BLAST of Spg008459 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 584.7 bits (1506), Expect = 1.0e-166
Identity = 317/700 (45.29%), Postives = 453/700 (64.71%), Query Frame = 0

Query: 8   LALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPKPFLMGS 67
           LA    V +Q +  C+ +C ++ I YPFG+S  CY   D++F I C    +  KP ++ S
Sbjct: 15  LAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITC----EEDKPNVL-S 74

Query: 68  NIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFTVIGCDTYA 127
           NIEV N +  G+L+ L   +  CY +   +     + R+   + S   NKFT++GC+ +A
Sbjct: 75  NIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFS-PNNKFTLVGCNAWA 134

Query: 128 YLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLE--IPKGLKYLDYEVKSFNNH 187
            L      ++Y++GC+  C  +T    +  C+G GCC+ E  IP     ++ +   F N 
Sbjct: 135 -LLSTFGIQNYSTGCMSLC--DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENM 194

Query: 188 TGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQD--RVPLVLDWGIKNDTCLTANNTDNCL 247
           T V +FNPC YAF +E   F FSS    +  ++  R P++LDW I N TC      +  +
Sbjct: 195 TSVEHFNPCSYAFFVEDGMFNFSSLEDLKDLRNVTRFPVLLDWSIGNQTCEQVVGRN--I 254

Query: 248 CGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKFHNCLSKDHCKDTNG 307
           CG NS      S  G  Y C+CL GF GNPYL  GCQ+INEC  + HNC     C++T G
Sbjct: 255 CGGNS--TCFDSTRGKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLG 314

Query: 308 SYTCYCPDGFGDGT-----LNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYKKW 367
           S+ C CP G    T     ++  +   K L    +++G  +GF ++L+  +++    +  
Sbjct: 315 SFHCQCPSGSDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHR 374

Query: 368 KFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGYGT 427
           K  + + +FF+ NGG +L Q LS    S   V+IFT+E +++AT+ ++ES ++G+GG GT
Sbjct: 375 KNTELRQQFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGT 434

Query: 428 VYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLLVY 487
           VYKG+L D S+VAIKK++L D+SQ  QFINEV+VLS+INHRNVVKLLGCCLETEVPLLVY
Sbjct: 435 VYKGILQDNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVY 494

Query: 488 EFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNILL 547
           EFI++GTLFDH+H     +SL+WE RL+IA E AG L+YLHS AS PIIHRD+K+ NILL
Sbjct: 495 EFISSGTLFDHLHGSMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILL 554

Query: 548 DHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLLE 607
           D N TAKV+DFGAS+L+P+DQ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+E
Sbjct: 555 DENLTAKVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLME 614

Query: 608 LITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKECL 667
           L++G+ A+ F+ P++ ++L  Y + AMKE+R+ ++++ ++  E N ++I+E A++A EC 
Sbjct: 615 LLSGEKALCFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECT 674

Query: 668 KVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEM 695
           ++ GEERP+MKEVA ELE LR    +H W+++    VE +
Sbjct: 675 RIMGEERPSMKEVAAELEALRVKTTKHQWSDQYPKEVEHL 701

BLAST of Spg008459 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 581.3 bits (1497), Expect = 1.1e-165
Identity = 319/725 (44.00%), Postives = 452/725 (62.34%), Query Frame = 0

Query: 8   LALAAAVASQAKPG--CEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPKPFLM 67
           LA    V  Q +PG  C++KC N+ I YPFG+S  CY   +E+F I C +     +P ++
Sbjct: 16  LACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE----DRPHVL 75

Query: 68  GSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPYLRVPMFTISNTKNKFTVIGCDT 127
            S+IEV N +  G+LQ+L   +  CY + G          +   ++S   NK T +GC+ 
Sbjct: 76  -SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLS-ANNKLTAVGCNA 135

Query: 128 YAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEVKS--FN 187
            + L      ++Y++ C+  C +  +   DG C+G GCC++++   L    +E  S    
Sbjct: 136 LS-LLDTFGMQNYSTACLSLCDSPPE--ADGECNGRGCCRVDVSAPLDSYTFETTSGRIK 195

Query: 188 NHTGVLNFNPCGYAFVIEQDNFTFSSS--YFREFPQDRVPLVLDWGIKNDTCLTANNTDN 247
           + T   +F+PC YAF++E D F FSS+          R P++LDW + N TC    +T  
Sbjct: 196 HMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGSTS- 255

Query: 248 CLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEGKF----HNCLSKDH 307
            +CG NS    S   +G  Y C+C +GF GNPYL  GCQ++NEC        HNC     
Sbjct: 256 -ICGGNSTCLDSTPRNG--YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKT 315

Query: 308 CKDTNGSYTCYCPDGFGDGTLNGTRCTSKSLSPVRIIVGVGVGFTVLLIGSTWMHLVYKK 367
           C++  G + C C  G+   T     C  K  +   I++   +GF V+L+G   +    K 
Sbjct: 316 CRNKVGGFYCKCQSGYRLDTTT-MSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKH 375

Query: 368 WKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFDESAVVGKGGYG 427
            K  + +++FF+ NGG +L Q LS    S   V+IFT++ ++KATN + ES ++G+GG G
Sbjct: 376 LKDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQG 435

Query: 428 TVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLGCCLETEVPLLV 487
           TVYKG+L D S+VAIKK++L D SQ  QFINEV+VLS+INHRNVVKLLGCCLETEVPLLV
Sbjct: 436 TVYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLV 495

Query: 488 YEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPIIHRDIKSTNIL 547
           YEFITNGTLFDH+H     +SL+WE RLKIA E AG L+YLHSSAS PIIHRDIK+ NIL
Sbjct: 496 YEFITNGTLFDHLHGSMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANIL 555

Query: 548 LDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLL 607
           LD N TAKV+DFGAS+L+P+D+ +L TMVQGTLGYLDPEY  T  L EKSDVYSFG+VL+
Sbjct: 556 LDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLM 615

Query: 608 ELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQIKEVAKLAKEC 667
           EL++G+ A+ F  P++ ++L  Y   A KE+R+++++   +  E+N K+I+E A++A EC
Sbjct: 616 ELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAARIAAEC 675

Query: 668 LKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMT--YLLDGASNSNSFVVGD 717
            ++ GEERP MKEVA +LE LR  + +H W+++     E +   ++L     ++S +  D
Sbjct: 676 TRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSSIGYD 726

BLAST of Spg008459 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 575.9 bits (1483), Expect = 4.7e-164
Identity = 323/739 (43.71%), Postives = 462/739 (62.52%), Query Frame = 0

Query: 2   VVKIAILALAAAVASQAKP--GCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDP 61
           +V I  LA    V  Q +P   C+ KC N+ I YPFG+S  CY   D+NF    N T   
Sbjct: 9   LVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNF----NLTCVV 68

Query: 62  PKPFLMGSNIEVTNISLYGELQILQYVARDCY-SKDGPSVNNRPYLRVPMFTISNTKNKF 121
            +  L+   I+VTNIS  G + +L     +CY  K+  +     Y     F++S + NKF
Sbjct: 69  EEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTALGYQLGSSFSLS-SNNKF 128

Query: 122 TVIGCDTYAYLYGELDGESYTSGCIGWCGNNTKLTRDGSCSGNGCCQLE---IPKGLKYL 181
           T++GC+  + L      ++Y++GC+  C  N++   +G C+G GCC  E   +P      
Sbjct: 129 TLVGCNALS-LLSTFGKQNYSTGCLSLC--NSQPEANGRCNGVGCCTTEDFSVPFDSDTF 188

Query: 182 DYEVKSFNN---------HTGVLNFNPCGYAFVIEQDNFTFSSSYFREFPQD--RVPLVL 241
            +      N         +T V  FNPC YAF++E   F F SS   +  ++  R P+ L
Sbjct: 189 QFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPVAL 248

Query: 242 DWGIKNDTCLTANNTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINE 301
           DW I N TC  A +T   +CG NS   +S + +G  Y C+C +G+ GNPY   GC++I+E
Sbjct: 249 DWSIGNQTCEQAGSTR--ICGKNSSCYNSTTRNG--YICKCNEGYDGNPYRSEGCKDIDE 308

Query: 302 CEGKFHNCLSKDHCKDTNGSYTCYCPDGFGDGTLNGT-RCTSKSLSPVRIIVGVGVGFTV 361
           C    HNC     C++ +G + C CP G+    LN +  CT       RI + + +G  V
Sbjct: 309 CISDTHNCSDPKTCRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIGVLV 368

Query: 362 LLIGSTWMHLVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATN 421
           LL+ +  +    K+ K+ + + +FF+ NGG +L Q LS    S    +IFT+E +++ATN
Sbjct: 369 LLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATN 428

Query: 422 KFDESAVVGKGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVK 481
            +DES ++G+GG GTVYKG+L D ++VAIKK++L D  Q  QFI+EV+VLS+INHRNVVK
Sbjct: 429 GYDESRILGQGGQGTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVK 488

Query: 482 LLGCCLETEVPLLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSAS 541
           +LGCCLETEVPLLVYEFITNGTLFDH+H     +SL+WE RL+IA E AG L+YLHSSAS
Sbjct: 489 ILGCCLETEVPLLVYEFITNGTLFDHLHGSIFDSSLTWEHRLRIAIEVAGTLAYLHSSAS 548

Query: 542 TPIIHRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSEL 601
            PIIHRDIK+ NILLD N TAKV+DFGASKL+P+D+ QL+TMVQGTLGYLDPEY  T  L
Sbjct: 549 IPIIHRDIKTANILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTTGLL 608

Query: 602 TEKSDVYSFGIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEEN 661
            EKSDVYSFG+VL+EL++G+ A+ F+ P+A ++L  Y + A +E+R+ ++++ ++  E+N
Sbjct: 609 NEKSDVYSFGVVLMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVLNEDN 668

Query: 662 FKQIKEVAKLAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANKNLSNVEEMT--YL 717
            K+I+E A++A EC ++ GEERP MKEVA +LE LR  + +H W+++     E +   ++
Sbjct: 669 LKEIQEAARIAAECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHI 728

BLAST of Spg008459 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 546.2 bits (1406), Expect = 4.0e-155
Identity = 307/704 (43.61%), Postives = 432/704 (61.36%), Query Frame = 0

Query: 2   VVKIAILALAAAVASQAKPGCEDKCDNLEIPYPFGMSKECYL--DENFFIECNKTYDPPK 61
           +V I  L+    V  Q  P C +KC N+ + YPFG S  C+   D +F + C        
Sbjct: 8   LVAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC------VN 67

Query: 62  PFLMGSNIEVTNISLYGELQILQYVARDCYSKDGPSVNNRPY-LRVPMFTISNTKNKFTV 121
             L    +EV  IS   +L++L   +  CY+  G       Y   +   T+S   N  T 
Sbjct: 68  ENLFYKGLEVVEISHSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSG-NNTITA 127

Query: 122 IGCDTYAYLYGELDGESYTS-GCIGWCGNNTKLTRDGSCSGNGCCQLEIPKGLKYLDYEV 181
           +GC++YA++    +G    S GCI  C +      +G C+G GCCQ  +P G  +L    
Sbjct: 128 LGCNSYAFV--SSNGTRRNSVGCISAC-DALSHEANGECNGEGCCQNPVPAGNNWLIVRS 187

Query: 182 KSFNNHTGV--LNFNPCGYAFVIEQDNFTFSSSYFREFPQDR---VPLVLDWGIKNDTCL 241
             F+N T V  ++   C YAF++E   F +++S    + Q+R    P+VLDW I+ +TC 
Sbjct: 188 YRFDNDTSVQPISEGQCIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETC- 247

Query: 242 TANNTDNCLCGPNSKRNSSLSVDGSEYYCQCLDGFQGNPYL--GCQNINECEG----KFH 301
                    CG N   ++S S  G  Y C+C  GFQGNPYL  GCQ+INEC        H
Sbjct: 248 --GQVGEKKCGVNGICSNSAS--GIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKH 307

Query: 302 NCLSKDHCKDTNGSYTCYCPDGFGDGTLNGTRCTSKS----LSPVRIIVGVGVGFTVLLI 361
           NC     C++  G + C C   +   T   T C  K     +    I++G  +GF V+L+
Sbjct: 308 NCSGDSTCENKLGHFRCNCRSRYELNTTTNT-CKPKGNPEYVEWTTIVLGTTIGFLVILL 367

Query: 362 GSTWMHLVYKKWKFIQRKDRFFKNNGGFLLQQHLSQGQSSTDSVRIFTQEELEKATNKFD 421
             + +    K  K  + + +FF+ NGG +L Q LS    S   V+IFT+E +++AT+ +D
Sbjct: 368 AISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYD 427

Query: 422 ESAVVGKGGYGTVYKGLLADGSVVAIKKSKLVDQSQTSQFINEVIVLSKINHRNVVKLLG 481
           E+ ++G+GG GTVYKG+L D S+VAIKK++L D SQ  QFINEV+VLS+INHRNVVKLLG
Sbjct: 428 ENRILGQGGQGTVYKGILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLG 487

Query: 482 CCLETEVPLLVYEFITNGTLFDHIHDKTKHASLSWEARLKIASETAGVLSYLHSSASTPI 541
           CCLETEVPLLVYEFI++GTLFDH+H     +SL+WE RL++A E AG L+YLHSSAS PI
Sbjct: 488 CCLETEVPLLVYEFISSGTLFDHLHGSMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPI 547

Query: 542 IHRDIKSTNILLDHNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 601
           IHRDIK+ NILLD N TAKV+DFGAS+L+P+D+  L+TMVQGTLGYLDPEY  T  L EK
Sbjct: 548 IHRDIKTANILLDENLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEK 607

Query: 602 SDVYSFGIVLLELITGKMAVRFDGPEAERNLAMYALCAMKEDRVEDVVEKRMATEENFKQ 661
           SDVYSFG+VL+EL++G+ A+ F+ P+  +++  Y   A KE+R+ ++++ ++  E N ++
Sbjct: 608 SDVYSFGVVLMELLSGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQRE 667

Query: 662 IKEVAKLAKECLKVKGEERPTMKEVAMELEGLRQMQIEHSWANK 687
           I++ A++A EC ++ GEERP MKEVA ELE LR  + +H W+++
Sbjct: 668 IQKAARIAVECTRLTGEERPGMKEVAAELEALRVTKTKHKWSDE 695