Spg008438 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008438
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionadenylate isopentenyltransferase-like
Locationscaffold10: 31559254 .. 31560165 (+)
RNA-Seq ExpressionSpg008438
SyntenySpg008438
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTCCTCCGCCCCTTCCGATAACCTCCTCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCCTCCGAGATCATCAATTCCGACAAAATGCAACTCTACCGCGGCCTCGACATCACCACCAACAAGCTCCCCTTCTCCGACCGCCGCGGCGTCCCCCACCACCTCCTCGGAGACTTCGACCCTCTAGCCGGCGAGGTCTCCGCCGCCGCCTTCCGCTCCCACGCCTCTACCGTCGTCTCCGGCGTCGTCTCCAGAGGGAAACTTCCGGTCGTCGTCGGTGGGTCTAACTCGTTCATCCACGCGCTTGTCACGGCGGATTTCGATCAGGACGACGACGTTTTCGCCGGGTCTCGGAATCGGGTCTCGGCGGAGTTTCGGTACCGGTGCTGCTTTCTGTGGGTCGACGTGTCGTTTCCGGTTTTGAAGCGGTACTTGTCGAAGCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGCCGAGTTGGCCGAGTTTTACGACCCGGAAATGGCCGAGTCGGAGCCCCGAGTCGGGCTGAGGAAGGCCATCGGAGTGCCGGAGTTCGAGGAGTTTTTCCGGCGGCATCACCCGAGAGGGAGGGAGTACAGAGAGGGGGATCCGCTGCGGGCGGGCGCATACGAAGAGGCGGTGAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGGGGAAGATCGGACGGCTGAGAAGGGCCGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTCTGGCGGTTCTGGCGGTTCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGAAACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGACCTTCATTCAGGAGGAGTAG

mRNA sequence

ATGCTCTCCTCCGCCCCTTCCGATAACCTCCTCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCCTCCGAGATCATCAATTCCGACAAAATGCAACTCTACCGCGGCCTCGACATCACCACCAACAAGCTCCCCTTCTCCGACCGCCGCGGCGTCCCCCACCACCTCCTCGGAGACTTCGACCCTCTAGCCGGCGAGGTCTCCGCCGCCGCCTTCCGCTCCCACGCCTCTACCGTCGTCTCCGGCGTCGTCTCCAGAGGGAAACTTCCGGTCGTCGTCGGTGGGTCTAACTCGTTCATCCACGCGCTTGTCACGGCGGATTTCGATCAGGACGACGACGTTTTCGCCGGGTCTCGGAATCGGGTCTCGGCGGAGTTTCGGTACCGGTGCTGCTTTCTGTGGGTCGACGTGTCGTTTCCGGTTTTGAAGCGGTACTTGTCGAAGCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGCCGAGTTGGCCGAGTTTTACGACCCGGAAATGGCCGAGTCGGAGCCCCGAGTCGGGCTGAGGAAGGCCATCGGAGTGCCGGAGTTCGAGGAGTTTTTCCGGCGGCATCACCCGAGAGGGAGGGAGTACAGAGAGGGGGATCCGCTGCGGGCGGGCGCATACGAAGAGGCGGTGAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGGGGAAGATCGGACGGCTGAGAAGGGCCGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTCTGGCGGTTCTGGCGGTTCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGAAACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGACCTTCATTCAGGAGGAGTAG

Coding sequence (CDS)

ATGCTCTCCTCCGCCCCTTCCGATAACCTCCTCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCCTCCGAGATCATCAATTCCGACAAAATGCAACTCTACCGCGGCCTCGACATCACCACCAACAAGCTCCCCTTCTCCGACCGCCGCGGCGTCCCCCACCACCTCCTCGGAGACTTCGACCCTCTAGCCGGCGAGGTCTCCGCCGCCGCCTTCCGCTCCCACGCCTCTACCGTCGTCTCCGGCGTCGTCTCCAGAGGGAAACTTCCGGTCGTCGTCGGTGGGTCTAACTCGTTCATCCACGCGCTTGTCACGGCGGATTTCGATCAGGACGACGACGTTTTCGCCGGGTCTCGGAATCGGGTCTCGGCGGAGTTTCGGTACCGGTGCTGCTTTCTGTGGGTCGACGTGTCGTTTCCGGTTTTGAAGCGGTACTTGTCGAAGCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGCCGAGTTGGCCGAGTTTTACGACCCGGAAATGGCCGAGTCGGAGCCCCGAGTCGGGCTGAGGAAGGCCATCGGAGTGCCGGAGTTCGAGGAGTTTTTCCGGCGGCATCACCCGAGAGGGAGGGAGTACAGAGAGGGGGATCCGCTGCGGGCGGGCGCATACGAAGAGGCGGTGAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGGGGAAGATCGGACGGCTGAGAAGGGCCGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTCTGGCGGTTCTGGCGGTTCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGAAACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGACCTTCATTCAGGAGGAGTAG

Protein sequence

MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFIQEE
Homology
BLAST of Spg008438 vs. NCBI nr
Match: KAG7032515.1 (hypothetical protein SDJN02_06564, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538.5 bits (1386), Expect = 3.7e-149
Identity = 276/302 (91.39%), Postives = 286/302 (94.70%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 36  MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 95

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA FD
Sbjct: 96  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFD 155

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NRVSAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 156 QDYDVFDGPLNRVSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 215

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLRAGAYEEAVRKIKENTWQLTK
Sbjct: 216 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTK 275

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 276 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 334

Query: 301 QE 303
           +E
Sbjct: 336 EE 334

BLAST of Spg008438 vs. NCBI nr
Match: KAG6601802.1 (hypothetical protein SDJN03_07035, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 538.5 bits (1386), Expect = 3.7e-149
Identity = 276/302 (91.39%), Postives = 286/302 (94.70%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 1   MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 60

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA FD
Sbjct: 61  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFD 120

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NRVSAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 121 QDYDVFDGPLNRVSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 180

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLRAGAYEEAVRKIKENTWQLTK
Sbjct: 181 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 241 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 299

Query: 301 QE 303
           +E
Sbjct: 301 EE 299

BLAST of Spg008438 vs. NCBI nr
Match: XP_022963367.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 538.5 bits (1386), Expect = 3.7e-149
Identity = 276/302 (91.39%), Postives = 286/302 (94.70%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 36  MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 95

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA FD
Sbjct: 96  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFD 155

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NRVSAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 156 QDYDVFDGPLNRVSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 215

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLRAGAYEEAVRKIKENTWQLTK
Sbjct: 216 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTK 275

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 276 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 334

Query: 301 QE 303
           +E
Sbjct: 336 EE 334

BLAST of Spg008438 vs. NCBI nr
Match: XP_022971768.1 (adenylate isopentenyltransferase-like [Cucurbita maxima])

HSP 1 Score: 535.0 bits (1377), Expect = 4.1e-148
Identity = 274/302 (90.73%), Postives = 285/302 (94.37%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 36  MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 95

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA  D
Sbjct: 96  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLD 155

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NRVSAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 156 QDYDVFDGPLNRVSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 215

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLR+GAYEEAVRKIKENTWQLTK
Sbjct: 216 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRSGAYEEAVRKIKENTWQLTK 275

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 276 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 334

Query: 301 QE 303
           +E
Sbjct: 336 EE 334

BLAST of Spg008438 vs. NCBI nr
Match: XP_023515564.1 (adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.0 bits (1377), Expect = 4.1e-148
Identity = 274/302 (90.73%), Postives = 285/302 (94.37%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 36  MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 95

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA  D
Sbjct: 96  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLD 155

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NR+SAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 156 QDYDVFDGPLNRLSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 215

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLRAGAYEEAVRKIKENTWQLTK
Sbjct: 216 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTK 275

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 276 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 334

Query: 301 QE 303
           +E
Sbjct: 336 EE 334

BLAST of Spg008438 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.6e-89
Identity = 183/303 (60.40%), Postives = 218/303 (71.95%), Query Frame = 0

Query: 8   DNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHHL 67
           + LL+L+GATG+GKS LSIDLAA FP E+INSDKMQ+Y+GLDITTNK+   DR GVPHHL
Sbjct: 30  EKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 89

Query: 68  LGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQD-DDVF 127
           LG+ DP  GE++ A FRS A   VS +  R KLPV+VGGSNSFIHAL+   FD     VF
Sbjct: 90  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVF 149

Query: 128 -AGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE----M 187
             GS + VS+E RY CCFLWVDVS  VL  YL+KRVDDMLE GM  ELAEFY PE     
Sbjct: 150 EEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHD 209

Query: 188 AESEPRVGLRKAIGVPEFEEFFRRHHPRGREYRE--GDPLRAGAYEEAVRKIKENTWQLT 247
            +S  R GLRKAIGVPEF+ +F +  P   E  +   D +R GA+EEAVR IKENT  L 
Sbjct: 210 EDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCHLA 269

Query: 248 KRQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTF 303
           KRQ+GKI RL+ AGWDL+R+DAT    +  A + S++ EK +EIWEKQV+EPSVKIV  F
Sbjct: 270 KRQIGKILRLKGAGWDLRRLDATE---SFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRF 329

BLAST of Spg008438 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 5.7e-84
Identity = 158/290 (54.48%), Postives = 212/290 (73.10%), Query Frame = 0

Query: 8   DNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHHL 67
           D ++++LGATG+GKS LS+DLA RFPSEIINSDK+Q+Y GL+ITTN++   DRRGVPHHL
Sbjct: 65  DKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHL 124

Query: 68  LGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVFA 127
           LG  +P  GE++A  FRS AS VV  + SR K+P++ GGSNSF+HAL+   FD   D F+
Sbjct: 125 LGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFS 184

Query: 128 GSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEPR 187
                +S++ RY CCF+WVDVS  VL  YL +RVD+M+++GM  EL+ FYDP  +  E R
Sbjct: 185 SGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETR 244

Query: 188 VGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIG 247
            G+RKAIGVPEF+ +F+ + P  ++  + D LR  AY++AV  IK NTW L KRQ+ KI 
Sbjct: 245 FGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIE 304

Query: 248 RLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVK 298
            L+ AGW+++RVDATA+  AV+  + S + +K  E WE+QV+EPSVKIVK
Sbjct: 305 MLKDAGWEIERVDATASFKAVM--MKSSSEKKWRENWEEQVLEPSVKIVK 351

BLAST of Spg008438 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 6.7e-77
Identity = 150/298 (50.34%), Postives = 212/298 (71.14%), Query Frame = 0

Query: 7   SDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHH 66
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+    DRRGVPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 67  LLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVF 126
           LLG+ +P AGEV+AA FR  A+  +S +  R KLP++ GGSNS+IHAL+   +D ++  F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 127 AGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEP 186
           +  +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  EP
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 187 RVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKI 246
            +G+ KAIGV EF+++ +  +    +  + DP+R  AYE+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 247 GRLRRAGWDLKRVDATAAVL-AVLAVLASE-AAEKRSEIWEKQVVEPSVKIVKTFIQE 303
            +LR AGWD+K+VDATA+   A+ A    E  AE + +IW K+V+EP VKIV++ + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

BLAST of Spg008438 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.1e-74
Identity = 151/299 (50.50%), Postives = 208/299 (69.57%), Query Frame = 0

Query: 8   DNLLLLLGATGSGKSSLSIDLAAR-FPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHH 67
           D ++L+ G TG+GKS LS+DLA R FP+EIINSDKMQ+Y+G +I TN +P  ++ GVPHH
Sbjct: 45  DKVVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHH 104

Query: 68  LLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVF 127
           LLG F P  GE++ A FRS A+  +S ++S  KLP+VVGGSNSF HAL+   FD D D F
Sbjct: 105 LLGQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPF 164

Query: 128 A--GSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAES 187
           +   S + + ++ RY+CC LWVDV  PVL ++L  RVD M+E+G+V +LAE YDP + +S
Sbjct: 165 SPGSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDS 224

Query: 188 EPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLG 247
             R+G+RK IGV EF+ +F R +P+  +    D  R  AYEE V+ +KE T +L K+Q  
Sbjct: 225 GRRLGVRKTIGVEEFDRYF-RVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKE 284

Query: 248 KIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRS-EIWEKQVVEPSVKIVKTFIQE 303
           KI +L R GW++KR+DATAA++A L    ++   K   EIWEK +V+ SV+IVK F+ E
Sbjct: 285 KIMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of Spg008438 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 144/296 (48.65%), Postives = 200/296 (67.57%), Query Frame = 0

Query: 10  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHHLLG 69
           +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +TTN++   +R GVPHHLLG
Sbjct: 45  VVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLG 104

Query: 70  DFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVFAGS 129
           +  P   E++ + FRS AS  +S + +RG LP++ GGSNSFIHAL+   FD     F+ S
Sbjct: 105 ELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFS-S 164

Query: 130 RNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEPRV- 189
              +S+  RY CCFLWVDVS  VL  YLSKRVD M+E+GM  ELA FYDP  + S  R  
Sbjct: 165 ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAH 224

Query: 190 GLRKAIGVPEFEEFFRRHHP-RGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIG 249
           G+ K IG+PEF+ +F  + P R ++  E D  R GAY+EAV++IKENTW+L K+Q+ +I 
Sbjct: 225 GIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIM 284

Query: 250 RLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFIQEE 304
           +L+ +GWD++R+DAT +                 EIW+  V++ S+K+VK F+ ++
Sbjct: 285 KLKSSGWDIQRLDATPSF-----------GRSSREIWDNTVLDESIKVVKRFLVKD 328

BLAST of Spg008438 vs. ExPASy TrEMBL
Match: A0A6J1HF40 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463593 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.8e-149
Identity = 276/302 (91.39%), Postives = 286/302 (94.70%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 36  MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 95

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA FD
Sbjct: 96  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFD 155

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NRVSAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 156 QDYDVFDGPLNRVSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 215

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLRAGAYEEAVRKIKENTWQLTK
Sbjct: 216 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTK 275

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 276 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 334

Query: 301 QE 303
           +E
Sbjct: 336 EE 334

BLAST of Spg008438 vs. ExPASy TrEMBL
Match: A0A6J1I7X6 (adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111470452 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.0e-148
Identity = 274/302 (90.73%), Postives = 285/302 (94.37%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           MLSS PSDNLLLLLGATGSGKSSLSID+AARFPSEIINSDKMQLYRGLD+TTNKLPFSDR
Sbjct: 36  MLSSLPSDNLLLLLGATGSGKSSLSIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDR 95

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           RGVPHHLLGDFDPLAGEVSAAAFRS AS V+SGVVSRG LPVVVGGSNS+IHALVTA  D
Sbjct: 96  RGVPHHLLGDFDPLAGEVSAAAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLD 155

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
           QD DVF G  NRVSAEFRYRCCFLWVDVSFPVLK+YLSKRVDDMLEAGMV ELAEFYDPE
Sbjct: 156 QDYDVFDGPLNRVSAEFRYRCCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPE 215

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MAE+EPRVGLRKAIGVPEFEEFFRR+HP+GREYREGDPLR+GAYEEAVRKIKENTWQLTK
Sbjct: 216 MAETEPRVGLRKAIGVPEFEEFFRRYHPKGREYREGDPLRSGAYEEAVRKIKENTWQLTK 275

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQLGKIGRLR AGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 276 RQLGKIGRLRMAGWDLKRVDATA---AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFI 334

Query: 301 QE 303
           +E
Sbjct: 336 EE 334

BLAST of Spg008438 vs. ExPASy TrEMBL
Match: A0A5D3CF13 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold383G00180 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.6e-143
Identity = 263/302 (87.09%), Postives = 280/302 (92.72%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           +LSSAPSD+LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLP S R
Sbjct: 22  VLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHR 81

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           R VPHHLLGD+DPLAGEVS AAFRSHAS V+SGV+SRG LP+VVGGSNSFIHALV ADFD
Sbjct: 82  RAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFIHALVAADFD 141

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
              DVF GSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVD+ML+ GM+ ELA+FYDPE
Sbjct: 142 DTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMLEELAQFYDPE 201

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MA+ E RVGLRKAIGVPEFEE+FRRHHP+GR+YREGDPL+A A+EEAVRKIKENTWQLTK
Sbjct: 202 MADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTK 261

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQL KIGRLRRAGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 262 RQLWKIGRLRRAGWDLKRVDATA---AVVAVLASEAAEKRSEIWETQVVEPSVKIVKGFI 320

Query: 301 QE 303
           QE
Sbjct: 322 QE 320

BLAST of Spg008438 vs. ExPASy TrEMBL
Match: A0A1S3BD73 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.6e-143
Identity = 263/302 (87.09%), Postives = 280/302 (92.72%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           +LSSAPSD+LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLP S R
Sbjct: 22  VLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHR 81

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           R VPHHLLGD+DPLAGEVS AAFRSHAS V+SGV+SRG LP+VVGGSNSFIHALV ADFD
Sbjct: 82  RAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFIHALVAADFD 141

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
              DVF GSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVD+ML+ GM+ ELA+FYDPE
Sbjct: 142 DTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMLEELAQFYDPE 201

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MA+ E RVGLRKAIGVPEFEE+FRRHHP+GR+YREGDPL+A A+EEAVRKIKENTWQLTK
Sbjct: 202 MADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTK 261

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQL KIGRLRRAGWDLKRVDATA   AV+AVLASEAAEKRSEIWE QVVEPSVKIVK FI
Sbjct: 262 RQLWKIGRLRRAGWDLKRVDATA---AVVAVLASEAAEKRSEIWETQVVEPSVKIVKGFI 320

Query: 301 QE 303
           QE
Sbjct: 322 QE 320

BLAST of Spg008438 vs. ExPASy TrEMBL
Match: A0A0A0K943 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.7e-142
Identity = 262/302 (86.75%), Postives = 278/302 (92.05%), Query Frame = 0

Query: 1   MLSSAPSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDR 60
           +LSSAPSD+LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLP S R
Sbjct: 22  ILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHR 81

Query: 61  RGVPHHLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFD 120
           R VPHHLLGDFDPLAGEVS AAFRSHAS V+SGV+SRG LP+VVGGSNSF+HALV ADFD
Sbjct: 82  RAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFD 141

Query: 121 QDDDVFAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPE 180
              DVF GSRNRVS EF YRCCFLWVDVSFPVLKRYLSKRVD+ML+ GMV ELA+FYDPE
Sbjct: 142 ATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPE 201

Query: 181 MAESEPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTK 240
           MA+SE RVGLRKAIGVPEFEE+FRRHHP+GR+YREGDPL+A A+EEAVRKIKENTWQLTK
Sbjct: 202 MADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQARAFEEAVRKIKENTWQLTK 261

Query: 241 RQLGKIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 300
           RQL KIGRLRRAGWDLKRVDATA   AV+AVLASE AEKRSEIWE QVVEPSVKIVK FI
Sbjct: 262 RQLWKIGRLRRAGWDLKRVDATA---AVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFI 320

Query: 301 QE 303
           QE
Sbjct: 322 QE 320

BLAST of Spg008438 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 312.4 bits (799), Expect = 4.0e-85
Identity = 158/290 (54.48%), Postives = 212/290 (73.10%), Query Frame = 0

Query: 8   DNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHHL 67
           D ++++LGATG+GKS LS+DLA RFPSEIINSDK+Q+Y GL+ITTN++   DRRGVPHHL
Sbjct: 65  DKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHL 124

Query: 68  LGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVFA 127
           LG  +P  GE++A  FRS AS VV  + SR K+P++ GGSNSF+HAL+   FD   D F+
Sbjct: 125 LGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFS 184

Query: 128 GSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEPR 187
                +S++ RY CCF+WVDVS  VL  YL +RVD+M+++GM  EL+ FYDP  +  E R
Sbjct: 185 SGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETR 244

Query: 188 VGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIG 247
            G+RKAIGVPEF+ +F+ + P  ++  + D LR  AY++AV  IK NTW L KRQ+ KI 
Sbjct: 245 FGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIE 304

Query: 248 RLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVK 298
            L+ AGW+++RVDATA+  AV+  + S + +K  E WE+QV+EPSVKIVK
Sbjct: 305 MLKDAGWEIERVDATASFKAVM--MKSSSEKKWRENWEEQVLEPSVKIVK 351

BLAST of Spg008438 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 288.9 bits (738), Expect = 4.8e-78
Identity = 150/298 (50.34%), Postives = 212/298 (71.14%), Query Frame = 0

Query: 7   SDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHH 66
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+    DRRGVPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 67  LLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVF 126
           LLG+ +P AGEV+AA FR  A+  +S +  R KLP++ GGSNS+IHAL+   +D ++  F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 127 AGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEP 186
           +  +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  EP
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 187 RVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKI 246
            +G+ KAIGV EF+++ +  +    +  + DP+R  AYE+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 247 GRLRRAGWDLKRVDATAAVL-AVLAVLASE-AAEKRSEIWEKQVVEPSVKIVKTFIQE 303
            +LR AGWD+K+VDATA+   A+ A    E  AE + +IW K+V+EP VKIV++ + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

BLAST of Spg008438 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 279.6 bits (714), Expect = 2.9e-75
Identity = 151/299 (50.50%), Postives = 208/299 (69.57%), Query Frame = 0

Query: 8   DNLLLLLGATGSGKSSLSIDLAAR-FPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHH 67
           D ++L+ G TG+GKS LS+DLA R FP+EIINSDKMQ+Y+G +I TN +P  ++ GVPHH
Sbjct: 45  DKVVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHH 104

Query: 68  LLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVF 127
           LLG F P  GE++ A FRS A+  +S ++S  KLP+VVGGSNSF HAL+   FD D D F
Sbjct: 105 LLGQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPF 164

Query: 128 A--GSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAES 187
           +   S + + ++ RY+CC LWVDV  PVL ++L  RVD M+E+G+V +LAE YDP + +S
Sbjct: 165 SPGSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDS 224

Query: 188 EPRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLG 247
             R+G+RK IGV EF+ +F R +P+  +    D  R  AYEE V+ +KE T +L K+Q  
Sbjct: 225 GRRLGVRKTIGVEEFDRYF-RVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKE 284

Query: 248 KIGRLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRS-EIWEKQVVEPSVKIVKTFIQE 303
           KI +L R GW++KR+DATAA++A L    ++   K   EIWEK +V+ SV+IVK F+ E
Sbjct: 285 KIMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of Spg008438 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 278.1 bits (710), Expect = 8.4e-75
Identity = 144/296 (48.65%), Postives = 200/296 (67.57%), Query Frame = 0

Query: 10  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPHHLLG 69
           +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +TTN++   +R GVPHHLLG
Sbjct: 45  VVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLG 104

Query: 70  DFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDVFAGS 129
           +  P   E++ + FRS AS  +S + +RG LP++ GGSNSFIHAL+   FD     F+ S
Sbjct: 105 ELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFS-S 164

Query: 130 RNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESEPRV- 189
              +S+  RY CCFLWVDVS  VL  YLSKRVD M+E+GM  ELA FYDP  + S  R  
Sbjct: 165 ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAH 224

Query: 190 GLRKAIGVPEFEEFFRRHHP-RGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIG 249
           G+ K IG+PEF+ +F  + P R ++  E D  R GAY+EAV++IKENTW+L K+Q+ +I 
Sbjct: 225 GIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIM 284

Query: 250 RLRRAGWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFIQEE 304
           +L+ +GWD++R+DAT +                 EIW+  V++ S+K+VK F+ ++
Sbjct: 285 KLKSSGWDIQRLDATPSF-----------GRSSREIWDNTVLDESIKVVKRFLVKD 328

BLAST of Spg008438 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 214.9 bits (546), Expect = 8.8e-56
Identity = 127/296 (42.91%), Postives = 182/296 (61.49%), Query Frame = 0

Query: 6   PSDNLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPFSDRRGVPH 65
           P D +++++GATG+GKS LS+D+A RF +EIINSDK+Q+++GLDI TNK+   +  GVPH
Sbjct: 39  PKDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPH 98

Query: 66  HLLGDFDPLAGEVSAAAFRSHASTVVSGVVSRGKLPVVVGGSNSFIHALVTADFDQDDDV 125
           HLLG   P A +++AA +   A+  +  V++RGKLP++VGGSNS++ ALV    D  ++ 
Sbjct: 99  HLLGVLPPEA-DLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALV----DDKENK 158

Query: 126 FAGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSKRVDDMLEAGMVAELAEFYDPEMAESE 185
           F           RY CCFLWVDV+ PVL  ++S+RVD M+E+GMV E+ EF+D   + S+
Sbjct: 159 FRS---------RYDCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFD--FSNSD 218

Query: 186 PRVGLRKAIGVPEFEEFFRRHHPRGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGK 245
              G++KAIG PEF+ FFR       E RE          + + +IK NT++L  RQ  K
Sbjct: 219 YSRGIKKAIGFPEFDRFFRNEQFLNVEDRE------ELLSKVLEEIKRNTFELACRQREK 278

Query: 246 IGRLRRA-GWDLKRVDATAAVLAVLAVLASEAAEKRSEIWEKQVVEPSVKIVKTFI 301
           I RLR+   W ++RVDAT       + + +  A      WE+ V  PS   V  F+
Sbjct: 279 IERLRKVKKWSIQRVDATPVFTKRRSKMDANVA------WERLVAGPSTDTVSRFL 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032515.13.7e-14991.39hypothetical protein SDJN02_06564, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6601802.13.7e-14991.39hypothetical protein SDJN03_07035, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022963367.13.7e-14991.39adenylate isopentenyltransferase-like [Cucurbita moschata][more]
XP_022971768.14.1e-14890.73adenylate isopentenyltransferase-like [Cucurbita maxima][more]
XP_023515564.14.1e-14890.73adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q5GHF72.6e-8960.40Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID35.7e-8454.48Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9SB606.7e-7750.34Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ... [more]
Q9C6L14.1e-7450.50Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9LJL41.2e-7348.65Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1HF401.8e-14991.39adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
A0A6J1I7X62.0e-14890.73adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11147045... [more]
A0A5D3CF135.6e-14387.09Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BD735.6e-14387.09adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE... [more]
A0A0A0K9434.7e-14286.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68460.14.0e-8554.48isopentenyltransferase 1 [more]
AT4G24650.14.8e-7850.34isopentenyltransferase 4 [more]
AT1G25410.12.9e-7550.50isopentenyltransferase 6 [more]
AT3G19160.18.4e-7548.65ATP/ADP isopentenyltransferases [more]
AT3G63110.18.8e-5642.91isopentenyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 150..256
e-value: 5.8E-122
score: 408.1
NoneNo IPR availablePFAMPF01715IPPTcoord: 43..121
e-value: 2.5E-17
score: 63.4
coord: 134..243
e-value: 6.0E-13
score: 49.1
NoneNo IPR availablePANTHERPTHR11088:SF65ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATEDcoord: 6..301
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 9..300
e-value: 5.8E-122
score: 408.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 10..184
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 6..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008438.1Spg008438.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity