Spg008243 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008243
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description(1->3)-beta-glucan endohydrolase
Locationscaffold2: 18976125 .. 18977111 (+)
RNA-Seq ExpressionSpg008243
SyntenySpg008243
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTATGATTAAGCAAATTTTTATTCAATTTCATGCAGCTGCTCAAATTGGTGTATGTTATGGACAACTTGGCAATGATCTACCATCTCCTGCAGAAGTTATCGACCTTTACAAAAAAAACAACATCCAAAGAATGCGACTTTACGCTCCAACCCATCCTACTTTCAATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTCGGCATTCCCAACCATGAGCTCAAAGACATGGCCACCATCCAAGGAAAAGCTCATTCATGGGTCCAACACAACATCCTCAACTTTGGTGATGTCAAATTCAAGTACATTGTTGTCGGAAACGAAATCAAAACTTACGATCATGCTGCTCAATTCCTCGTACCAGCCATGCAAAACATCCAAAATGCAATTTCAGCCGTGGGCTTGAGAGGTAAAATCAAAGTTTCAACTTCATTCGACACCGGAATTCTTGCAGAGTCCTTCCCTCCTTCAAAGGGCTCCTTCAGAGGAGACTATCTTCCAATTCTTGACCCGACCATTCGCTTCCTCGTCGACAACAACTCGCCGTTGCTCGTCAACGTGTACCCTTACTTCAGCTATATCGATAACACTGCAGAAATTTCTCTCGATTATGCACTTTTTAGAGGGACGCCGGTTGTTGCAGATGACCAACTTGTTTATCGCAATTTGTTTGATGCTATTTTGGATGCTGTTTACTCTGCTTTGGAGAAATCTGGCGGTGGAGGTCTGGAAATTGTTGTATCGGAGACTGGTTGGCCAACAGATCGTGGGGTGGCGGCGAACGTAGATAATGCAAGGACTTACATCAACAATCTAATTCAACATGTGAAAGTAGGGACACCGAAGCGACCGAGAAGGCCTGTTGAGACCTACGTGTTTGCGATGTTCGATGAGAATGAGAAAACAACTCCTCCTGAAGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAACCCAAATACCATGTCAACTTCAACTGA

mRNA sequence

ATGTCTATGATTAAGCAAATTTTTATTCAATTTCATGCAGCTGCTCAAATTGGTGTATGTTATGGACAACTTGGCAATGATCTACCATCTCCTGCAGAAGTTATCGACCTTTACAAAAAAAACAACATCCAAAGAATGCGACTTTACGCTCCAACCCATCCTACTTTCAATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTCGGCATTCCCAACCATGAGCTCAAAGACATGGCCACCATCCAAGGAAAAGCTCATTCATGGGTCCAACACAACATCCTCAACTTTGGTGATGTCAAATTCAAGTACATTGTTGTCGGAAACGAAATCAAAACTTACGATCATGCTGCTCAATTCCTCGTACCAGCCATGCAAAACATCCAAAATGCAATTTCAGCCGTGGGCTTGAGAGGTAAAATCAAAGTTTCAACTTCATTCGACACCGGAATTCTTGCAGAGTCCTTCCCTCCTTCAAAGGGCTCCTTCAGAGGAGACTATCTTCCAATTCTTGACCCGACCATTCGCTTCCTCGTCGACAACAACTCGCCGTTGCTCGTCAACGTGTACCCTTACTTCAGCTATATCGATAACACTGCAGAAATTTCTCTCGATTATGCACTTTTTAGAGGGACGCCGGTTGTTGCAGATGACCAACTTGTTTATCGCAATTTGTTTGATGCTATTTTGGATGCTGTTTACTCTGCTTTGGAGAAATCTGGCGGTGGAGGTCTGGAAATTGTTGTATCGGAGACTGGTTGGCCAACAGATCGTGGGGTGGCGGCGAACGTAGATAATGCAAGGACTTACATCAACAATCTAATTCAACATGTGAAAGTAGGGACACCGAAGCGACCGAGAAGGCCTGTTGAGACCTACGTGTTTGCGATGTTCGATGAGAATGAGAAAACAACTCCTCCTGAAGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAACCCAAATACCATGTCAACTTCAACTGA

Coding sequence (CDS)

ATGTCTATGATTAAGCAAATTTTTATTCAATTTCATGCAGCTGCTCAAATTGGTGTATGTTATGGACAACTTGGCAATGATCTACCATCTCCTGCAGAAGTTATCGACCTTTACAAAAAAAACAACATCCAAAGAATGCGACTTTACGCTCCAACCCATCCTACTTTCAATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTCGGCATTCCCAACCATGAGCTCAAAGACATGGCCACCATCCAAGGAAAAGCTCATTCATGGGTCCAACACAACATCCTCAACTTTGGTGATGTCAAATTCAAGTACATTGTTGTCGGAAACGAAATCAAAACTTACGATCATGCTGCTCAATTCCTCGTACCAGCCATGCAAAACATCCAAAATGCAATTTCAGCCGTGGGCTTGAGAGGTAAAATCAAAGTTTCAACTTCATTCGACACCGGAATTCTTGCAGAGTCCTTCCCTCCTTCAAAGGGCTCCTTCAGAGGAGACTATCTTCCAATTCTTGACCCGACCATTCGCTTCCTCGTCGACAACAACTCGCCGTTGCTCGTCAACGTGTACCCTTACTTCAGCTATATCGATAACACTGCAGAAATTTCTCTCGATTATGCACTTTTTAGAGGGACGCCGGTTGTTGCAGATGACCAACTTGTTTATCGCAATTTGTTTGATGCTATTTTGGATGCTGTTTACTCTGCTTTGGAGAAATCTGGCGGTGGAGGTCTGGAAATTGTTGTATCGGAGACTGGTTGGCCAACAGATCGTGGGGTGGCGGCGAACGTAGATAATGCAAGGACTTACATCAACAATCTAATTCAACATGTGAAAGTAGGGACACCGAAGCGACCGAGAAGGCCTGTTGAGACCTACGTGTTTGCGATGTTCGATGAGAATGAGAAAACAACTCCTCCTGAAGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAACCCAAATACCATGTCAACTTCAACTGA

Protein sequence

MSMIKQIFIQFHAAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN
Homology
BLAST of Spg008243 vs. NCBI nr
Match: XP_022961079.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata])

HSP 1 Score: 536.2 bits (1380), Expect = 2.0e-148
Identity = 262/330 (79.39%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 1   MSMIKQIFIQFH-AAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNAL 60
           +S++  +F   H  +AQIGVCYGQLGNDLPSP EVI+LYK+NNIQRMRLYA  HPTF+AL
Sbjct: 16  LSLLWLLFASMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDAL 75

Query: 61  RGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQF 120
           RGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYIVVGNEIKTYD AA F
Sbjct: 76  RGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASF 135

Query: 121 LVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVD 180
           LVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ DYLPIL+PTIRFL+D
Sbjct: 136 LVPAMQNIQNAISAVGLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLD 195

Query: 181 NNSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEK 240
           NN+PLLVNVYPYFSY+ NTA+IS+DYALFRG +P V D Q VY NLFDAILDAVYSALEK
Sbjct: 196 NNAPLLVNVYPYFSYVANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEK 255

Query: 241 SGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFD 300
           +GG GLEIVVSETGWPT+ G AA V+NA TY NNLIQHVK GTPKR  +PVETYVFAMFD
Sbjct: 256 AGGDGLEIVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFD 315

Query: 301 ENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 329
           ENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 316 ENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Spg008243 vs. NCBI nr
Match: KAG6590085.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 533.9 bits (1374), Expect = 9.9e-148
Identity = 260/315 (82.54%), Postives = 284/315 (90.16%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQIGVCYGQLGNDLPSP EVI+LYK+NNIQRMRLYAP HPTF+ALRGSNIELMLGIPN+E
Sbjct: 78  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPNHPTFDALRGSNIELMLGIPNNE 137

Query: 75  LKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 134
           L+D+A  Q KA SWVQ N+++FGDVKFKYIVVGNEIKTYD AA FLVPAMQNIQNAISAV
Sbjct: 138 LEDLANFQEKADSWVQDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAISAV 197

Query: 135 GLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSY 194
           GL G+IKVST+FDTGILAES+PPSKGSF+ DYLPIL+PTIRFL+DNN+PLLVNVYPYFSY
Sbjct: 198 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 257

Query: 195 IDNTAEISLDYALFRGT-PVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGW 254
           + NTA+IS+DYALFRGT P V D Q VY NLFDAILDAVYSALE SGG  +EIVVSETGW
Sbjct: 258 VANTADISMDYALFRGTSPSVVDGQFVYHNLFDAILDAVYSALETSGGDRVEIVVSETGW 317

Query: 255 PTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWG 314
           PT+ G AA V+NA TY NNLIQHVK GTPKR  +PVETYVFAMFDENEKTTPPEVEKHWG
Sbjct: 318 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 377

Query: 315 LFSPNKQPKYHVNFN 329
           LFSP+K PKY VNFN
Sbjct: 378 LFSPSKLPKYEVNFN 392

BLAST of Spg008243 vs. NCBI nr
Match: XP_022987103.1 (probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima])

HSP 1 Score: 533.1 bits (1372), Expect = 1.7e-147
Identity = 261/330 (79.09%), Postives = 291/330 (88.18%), Query Frame = 0

Query: 1   MSMIKQIFIQFH-AAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNAL 60
           +S++  +F + H  +AQIGVCYGQLGNDLPSP EVI+LYK+NNIQRMRLYAP HPTF+AL
Sbjct: 16  VSLLWLLFARMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDAL 75

Query: 61  RGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQF 120
           RGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYIVVGNEIKT D AA F
Sbjct: 76  RGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASF 135

Query: 121 LVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVD 180
           LVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ DYLPIL+PTIRFL+D
Sbjct: 136 LVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLD 195

Query: 181 NNSPLLVNVYPYFSYIDNTAEISLDYALFRGT-PVVADDQLVYRNLFDAILDAVYSALEK 240
           NN+PLLVNVYPYFSY+ NTA+IS+DYALFRGT P V D Q VY NLFDAILDAVYSALEK
Sbjct: 196 NNAPLLVNVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEK 255

Query: 241 SGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFD 300
           +G  GLEI VSETGWPT+ G AA V+NA TY NNLIQHVK GTPKR  +PVETYVFAMFD
Sbjct: 256 AGSDGLEIAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFD 315

Query: 301 ENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 329
           ENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 316 ENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Spg008243 vs. NCBI nr
Match: XP_022139185.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 531.2 bits (1367), Expect = 6.4e-147
Identity = 258/314 (82.17%), Postives = 281/314 (89.49%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQIGVCYGQLG+DLPSP EVIDLY +NNI+RMRLYAP  P+ NALRGSNIELMLGIPNHE
Sbjct: 32  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 91

Query: 75  LKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 134
           L+ MAT Q  A+SW Q N+L+FGDVKFKYIVVGNEIK  D AA+FLVPAMQNIQNAISA 
Sbjct: 92  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 151

Query: 135 GLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSY 194
           GLRGKIKVST+ DTG+LAES+PPSKGSFR DYLPILDPTIRFLVDNNSPLL+N+Y YFS+
Sbjct: 152 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 211

Query: 195 IDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGWP 254
           I NT +ISLDYALF G  +V+D QLVY+NLFDAILDAVYSALEKS GGGLEIVVSE+GWP
Sbjct: 212 ISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGWP 271

Query: 255 TDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWGL 314
           TD G AA VDNARTY NNLIQHVK GTPKRP  P+ETYVFAMFDEN+KTTPPEVEKHWGL
Sbjct: 272 TDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWGL 331

Query: 315 FSPNKQPKYHVNFN 329
           FSPNKQPKY VNF+
Sbjct: 332 FSPNKQPKYLVNFH 345

BLAST of Spg008243 vs. NCBI nr
Match: XP_038895085.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 508.4 bits (1308), Expect = 4.5e-140
Identity = 246/316 (77.85%), Postives = 277/316 (87.66%), Query Frame = 0

Query: 13  AAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPN 72
           A A+IGVCYGQLGNDLPSP EVI+LYK+NNIQRMRLYAP  PTF+ALRGSNIELMLGIPN
Sbjct: 31  AGAEIGVCYGQLGNDLPSPQEVIELYKQNNIQRMRLYAPNQPTFDALRGSNIELMLGIPN 90

Query: 73  HELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAIS 132
            +++ MA  Q  A++WVQ NILNF DVKFKYIVVGNEIKT + AA FLVPAMQNIQNAIS
Sbjct: 91  DQIRSMAATQDNANAWVQENILNFPDVKFKYIVVGNEIKTNEEAATFLVPAMQNIQNAIS 150

Query: 133 AVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYF 192
           AVGL+G+IKVST+FDTGIL++SFPPS+GSF  +YLPIL+PT RFL+DN+SPLLVN+YPYF
Sbjct: 151 AVGLQGQIKVSTAFDTGILSQSFPPSQGSFDTNYLPILNPTFRFLLDNSSPLLVNLYPYF 210

Query: 193 SYIDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETG 252
           SY+  T  + LDYA+F GT ++ D +L Y+NLFDAILDAVYSALEKSGGG LEIVVSETG
Sbjct: 211 SYV-TTPRMGLDYAIFTGTSLIGDGELNYQNLFDAILDAVYSALEKSGGGSLEIVVSETG 270

Query: 253 WPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHW 312
           WPTD G AA VDNARTY NNLIQHV  GTPKR  R +ETY+FAMFDENEKTTPPEVE+HW
Sbjct: 271 WPTDHGDAATVDNARTYNNNLIQHVNQGTPKRRGRAIETYLFAMFDENEKTTPPEVERHW 330

Query: 313 GLFSPNKQPKYHVNFN 329
           GLFSPNKQPKY VNFN
Sbjct: 331 GLFSPNKQPKYSVNFN 345

BLAST of Spg008243 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 2.7e-116
Identity = 202/318 (63.52%), Postives = 244/318 (76.73%), Query Frame = 0

Query: 12  HAAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIP 71
           H  A IGVC G +G+DLP  AEV+ LYK NNI RMRLY P      ALRGSNI+L+LG+P
Sbjct: 35  HTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVP 94

Query: 72  NHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAI 131
           N  L+ +A  Q  A++WVQ+N+ N+ +VKFKYI VGNE+K  D  AQFLVPAM+NIQ AI
Sbjct: 95  NENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAI 154

Query: 132 SAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPY 191
           S  GL  KIKVST+ DTG+L E+FPPS GSF+ +Y  +L P IRFLV + SPLLVN+YPY
Sbjct: 155 SLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPY 214

Query: 192 FSYIDNTAEISLDYALFRGTPVVADD-QLVYRNLFDAILDAVYSALEKSGGGGLEIVVSE 251
           F+Y  NT +I LDYALF    VV  D    YRNLFDA+LD VY+ALEK+GGG L++V+SE
Sbjct: 215 FAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISE 274

Query: 252 TGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEK 311
           TGWP+  G A  +DNART+I+NLIQHVK GTP+RP RP+ETY+FAMFDEN KT  PE+EK
Sbjct: 275 TGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKT--PELEK 334

Query: 312 HWGLFSPNKQPKYHVNFN 329
           HWGLFSP KQPKY ++FN
Sbjct: 335 HWGLFSPTKQPKYQISFN 350

BLAST of Spg008243 vs. ExPASy Swiss-Prot
Match: Q9M2M0 (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN=BG1 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.8e-113
Identity = 195/314 (62.10%), Postives = 246/314 (78.34%), Query Frame = 0

Query: 16  QIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHEL 75
           Q+GVCYG+ GN+LPSPAE I L+K+ NIQR+RLY+P H    ALRGSNIE+ LG+PN  L
Sbjct: 26  QVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYL 85

Query: 76  KDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 135
           + +A+ Q +A++WVQ  ++N+ + V+F+YI VGNE+K  D  AQFLVPAM+NI  A+ A 
Sbjct: 86  QSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAA 145

Query: 136 GLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSY 195
           GL G+IKVSTS D G+L ES+PPSKGSFRGD + +++P IRFLV  NSPLL+N+Y YFSY
Sbjct: 146 GLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 205

Query: 196 IDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGW 255
             N  +I LDYALF   + +V+D    Y+NLFDA+LDA+YSALEKSGG  LEIVV+ETGW
Sbjct: 206 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 265

Query: 256 PTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWG 315
           PT  G   N++NAR Y NNLI+HVK GTPKRP + +ETY+FA++DEN+K TPP VEK WG
Sbjct: 266 PTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFWG 325

Query: 316 LFSPNKQPKYHVNF 328
           LF PNKQPKY +NF
Sbjct: 326 LFYPNKQPKYDINF 339

BLAST of Spg008243 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.6e-108
Identity = 199/318 (62.58%), Postives = 243/318 (76.42%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQ GVCYG+LGN+LP+P EV+ LY + NI+RMR+Y P+     ALRGSNIEL+L IPN  
Sbjct: 32  AQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDIPNDN 91

Query: 75  LKDMATIQGKAHSWVQHNILNF-GDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISA 134
           L+++A+ Q  A+ WVQ NI N+  +V+F+Y+ VGNE+K     AQFLVPA++NIQ AIS 
Sbjct: 92  LRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAISN 151

Query: 135 VGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYL-PILDPTIRFLVDNNSPLLVNVYPYF 194
            GL  ++KVST+ DTG LAESFPPSKGSF+ DY    LD  IRFLV+NN+PL+VNVY YF
Sbjct: 152 AGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYSYF 211

Query: 195 SYIDNTAEISLDYALFRGTPVVADD-QLVYRNLFDAILDAVYSALEKSGGGGLEIVVSET 254
           +Y  N  +ISLDYALFR   VV  D  L YRNLFDA +DAVY+ALEK+GGG L IVVSE+
Sbjct: 212 AYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVSES 271

Query: 255 GWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKH 314
           GWP+  G A ++DNARTY  NL+++VK GTPKRP  P+ETYVFAMFDEN+K   PE EK 
Sbjct: 272 GWPSSGGTATSLDNARTYNTNLVRNVKQGTPKRPGAPLETYVFAMFDENQK--QPEFEKF 331

Query: 315 WGLFSP-NKQPKYHVNFN 329
           WGLFSP  KQPKY +NFN
Sbjct: 332 WGLFSPITKQPKYSINFN 347

BLAST of Spg008243 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.5e-102
Identity = 184/319 (57.68%), Postives = 238/319 (74.61%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQ GVCYG+ GN LPSPA+V+ L  +NNI+RMR+Y P  PT  ALRGSNIELMLG+PN +
Sbjct: 24  AQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTLEALRGSNIELMLGVPNPD 83

Query: 75  LKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQF---LVPAMQNIQNAI 134
           L+++A  Q  A +WVQ+N+ N+G+VKF+YI VGNE+   +  +++   L+ AM+NIQ AI
Sbjct: 84  LENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNENSKYVPVLLNAMRNIQTAI 143

Query: 135 SAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPY 194
           S  GL  +IKVST+ +TG+  ++ PPS G F+ D    ++P I FLV N +PLLVN+YPY
Sbjct: 144 SGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEPIINFLVTNRAPLLVNLYPY 203

Query: 195 FSYIDNTAEISLDYALFRGTPVVADDQ-LVYRNLFDAILDAVYSALEKSGGGGLEIVVSE 254
           F+ I N A+I L+YALF  + VV +D    YRNLFDAILDA YSALEK+ G  LEIVVSE
Sbjct: 204 FA-IANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDATYSALEKASGSSLEIVVSE 263

Query: 255 TGWPT-DRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVE 314
           +GWP+   G   ++DNARTY NNLI HVK G+PKRP  P+ETYVFA+FDE++K   PE+E
Sbjct: 264 SGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIETYVFALFDEDQK--DPEIE 323

Query: 315 KHWGLFSPNKQPKYHVNFN 329
           KH+GLFS N QPKY ++FN
Sbjct: 324 KHFGLFSANMQPKYQISFN 339

BLAST of Spg008243 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-99
Identity = 181/319 (56.74%), Postives = 230/319 (72.10%), Query Frame = 0

Query: 14  AAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNH 73
           A  +GVCYG LGN+LP  ++V+ LYK  NI+RMRLY P      ALRGSNIE+MLG+PN 
Sbjct: 30  AQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVMLGVPNS 89

Query: 74  ELKDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIKTY---DHAAQFLVPAMQNIQN 133
           +L+++A     A++WVQ N+ NF   VKF+YI VGNE+          ++L+PAM+NI+N
Sbjct: 90  DLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAMRNIRN 149

Query: 134 AISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVY 193
           AIS+ GL+  IKVS+S D  ++  SFPPS+GSFR D    +DP I F+   NSPLLVN+Y
Sbjct: 150 AISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPIIGFVRRINSPLLVNIY 209

Query: 194 PYFSYIDNTAEISLDYALFRGTPVVADD-QLVYRNLFDAILDAVYSALEKSGGGGLEIVV 253
           PYFSY  N  +ISL YALF    VV  D  L YRNLFDA+ DAVY+AL ++GGG +EIVV
Sbjct: 210 PYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAGGGSIEIVV 269

Query: 254 SETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEV 313
           SE+GWP+    AA  +NA TY  NLIQHVK G+P+RP + +ETY+FAMFDEN K   PE+
Sbjct: 270 SESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETYLFAMFDENNKN--PEL 329

Query: 314 EKHWGLFSPNKQPKYHVNF 328
           EKH+GLFSPNKQPKY ++F
Sbjct: 330 EKHFGLFSPNKQPKYPLSF 346

BLAST of Spg008243 vs. ExPASy TrEMBL
Match: A0A6J1HCY3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 9.7e-149
Identity = 262/330 (79.39%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 1   MSMIKQIFIQFH-AAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNAL 60
           +S++  +F   H  +AQIGVCYGQLGNDLPSP EVI+LYK+NNIQRMRLYA  HPTF+AL
Sbjct: 16  LSLLWLLFASMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDAL 75

Query: 61  RGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQF 120
           RGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYIVVGNEIKTYD AA F
Sbjct: 76  RGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASF 135

Query: 121 LVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVD 180
           LVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ DYLPIL+PTIRFL+D
Sbjct: 136 LVPAMQNIQNAISAVGLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLD 195

Query: 181 NNSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEK 240
           NN+PLLVNVYPYFSY+ NTA+IS+DYALFRG +P V D Q VY NLFDAILDAVYSALEK
Sbjct: 196 NNAPLLVNVYPYFSYVANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEK 255

Query: 241 SGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFD 300
           +GG GLEIVVSETGWPT+ G AA V+NA TY NNLIQHVK GTPKR  +PVETYVFAMFD
Sbjct: 256 AGGDGLEIVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFD 315

Query: 301 ENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 329
           ENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 316 ENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Spg008243 vs. ExPASy TrEMBL
Match: A0A6J1JIH6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.2e-148
Identity = 261/330 (79.09%), Postives = 291/330 (88.18%), Query Frame = 0

Query: 1   MSMIKQIFIQFH-AAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNAL 60
           +S++  +F + H  +AQIGVCYGQLGNDLPSP EVI+LYK+NNIQRMRLYAP HPTF+AL
Sbjct: 16  VSLLWLLFARMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDAL 75

Query: 61  RGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQF 120
           RGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYIVVGNEIKT D AA F
Sbjct: 76  RGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASF 135

Query: 121 LVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVD 180
           LVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ DYLPIL+PTIRFL+D
Sbjct: 136 LVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLD 195

Query: 181 NNSPLLVNVYPYFSYIDNTAEISLDYALFRGT-PVVADDQLVYRNLFDAILDAVYSALEK 240
           NN+PLLVNVYPYFSY+ NTA+IS+DYALFRGT P V D Q VY NLFDAILDAVYSALEK
Sbjct: 196 NNAPLLVNVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEK 255

Query: 241 SGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFD 300
           +G  GLEI VSETGWPT+ G AA V+NA TY NNLIQHVK GTPKR  +PVETYVFAMFD
Sbjct: 256 AGSDGLEIAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFD 315

Query: 301 ENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 329
           ENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 316 ENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

BLAST of Spg008243 vs. ExPASy TrEMBL
Match: A0A6J1CBL7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.1e-147
Identity = 258/314 (82.17%), Postives = 281/314 (89.49%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQIGVCYGQLG+DLPSP EVIDLY +NNI+RMRLYAP  P+ NALRGSNIELMLGIPNHE
Sbjct: 32  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 91

Query: 75  LKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 134
           L+ MAT Q  A+SW Q N+L+FGDVKFKYIVVGNEIK  D AA+FLVPAMQNIQNAISA 
Sbjct: 92  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 151

Query: 135 GLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSY 194
           GLRGKIKVST+ DTG+LAES+PPSKGSFR DYLPILDPTIRFLVDNNSPLL+N+Y YFS+
Sbjct: 152 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 211

Query: 195 IDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGWP 254
           I NT +ISLDYALF G  +V+D QLVY+NLFDAILDAVYSALEKS GGGLEIVVSE+GWP
Sbjct: 212 ISNTKDISLDYALFTGMSMVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGWP 271

Query: 255 TDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWGL 314
           TD G AA VDNARTY NNLIQHVK GTPKRP  P+ETYVFAMFDEN+KTTPPEVEKHWGL
Sbjct: 272 TDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWGL 331

Query: 315 FSPNKQPKYHVNFN 329
           FSPNKQPKY VNF+
Sbjct: 332 FSPNKQPKYLVNFH 345

BLAST of Spg008243 vs. ExPASy TrEMBL
Match: A0A1S3C9Z3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 9.4e-136
Identity = 244/315 (77.46%), Postives = 269/315 (85.40%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQIGVCYGQLGNDLPSPAEVIDLY +NNIQRMRLYAP   TFNALRGS+IELMLG+PN E
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQATFNALRGSSIELMLGLPNDE 92

Query: 75  LKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 134
           ++ MA  Q  A++WVQ NILNF DV FKYIVVGNEIKT + AA+FLVPAMQNIQNAISAV
Sbjct: 93  IQSMAATQDNANAWVQDNILNFPDVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 135 GLRGKIKVSTSFDTGILA-ESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFS 194
           GL GKIKVST+F TGIL+ ESFPPS GSF   YLPIL+PTIRFL+DNNSPLLVN+YPYFS
Sbjct: 153 GLGGKIKVSTAFHTGILSVESFPPSHGSFDASYLPILNPTIRFLLDNNSPLLVNLYPYFS 212

Query: 195 YIDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGW 254
           Y+  T  + LDYA+F GT +V D +  Y+NLF AILD VYSALEK+GGG LEIVVSETGW
Sbjct: 213 YV-GTPSMELDYAIFTGTSLVVDGEFNYQNLFHAILDTVYSALEKNGGGSLEIVVSETGW 272

Query: 255 PTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWG 314
           PTD G AA VDNARTY NNLIQ VK GTPKR  R +ETYVFAMFDENEKTTPPEVE+HWG
Sbjct: 273 PTDGGEAATVDNARTYTNNLIQRVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 315 LFSPNKQPKYHVNFN 329
           LFSPNKQPK+ V+FN
Sbjct: 333 LFSPNKQPKFSVSFN 346

BLAST of Spg008243 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 5.1e-134
Identity = 239/314 (76.11%), Postives = 270/314 (85.99%), Query Frame = 0

Query: 15  AQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHE 74
           AQIGVCYGQLGNDLPSPAEVIDLY +NNIQRMRLYAP   TFNALRGS+IELMLG+PN +
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTFNALRGSSIELMLGLPNDQ 92

Query: 75  LKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 134
           ++ MA  Q  A++W+Q NILNF DV FKYIVVGNEIKT + AA+FLVPAMQNIQNAISAV
Sbjct: 93  IQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 135 GLRGKIKVSTSFDTGIL-AESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFS 194
           GL+G+IKVST+F TGIL AESFPPS GSF  +YLPIL+PTIRFL+DNNSPLL+N+YPYFS
Sbjct: 153 GLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYFS 212

Query: 195 YIDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGW 254
           Y+  T  + LDYA+F GT +V D +  Y+NLFDAILD VYSALEK+GGG LE+VVSETGW
Sbjct: 213 YV-ATPNMELDYAIFTGTSLVEDGEFNYQNLFDAILDTVYSALEKNGGGSLEVVVSETGW 272

Query: 255 PTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWG 314
           PT+ G AA VDNARTY NNLIQHVK GTPKR  R +ETYVFAMFDENEKTTPPEVE+HWG
Sbjct: 273 PTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWG 332

Query: 315 LFSPNKQPKYHVNF 328
           LFSPNKQPK  + F
Sbjct: 333 LFSPNKQPKSFLCF 345

BLAST of Spg008243 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 409.1 bits (1050), Expect = 3.4e-114
Identity = 195/314 (62.10%), Postives = 246/314 (78.34%), Query Frame = 0

Query: 16  QIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHEL 75
           Q+GVCYG+ GN+LPSPAE I L+K+ NIQR+RLY+P H    ALRGSNIE+ LG+PN  L
Sbjct: 26  QVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYL 85

Query: 76  KDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAV 135
           + +A+ Q +A++WVQ  ++N+ + V+F+YI VGNE+K  D  AQFLVPAM+NI  A+ A 
Sbjct: 86  QSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAA 145

Query: 136 GLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSY 195
           GL G+IKVSTS D G+L ES+PPSKGSFRGD + +++P IRFLV  NSPLL+N+Y YFSY
Sbjct: 146 GLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 205

Query: 196 IDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGW 255
             N  +I LDYALF   + +V+D    Y+NLFDA+LDA+YSALEKSGG  LEIVV+ETGW
Sbjct: 206 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 265

Query: 256 PTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWG 315
           PT  G   N++NAR Y NNLI+HVK GTPKRP + +ETY+FA++DEN+K TPP VEK WG
Sbjct: 266 PTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFWG 325

Query: 316 LFSPNKQPKYHVNF 328
           LF PNKQPKY +NF
Sbjct: 326 LFYPNKQPKYDINF 339

BLAST of Spg008243 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 362.5 bits (929), Expect = 3.7e-100
Identity = 186/318 (58.49%), Postives = 230/318 (72.33%), Query Frame = 0

Query: 13  AAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPN 72
           A  QIGVCYG+ GN+L   +EV+ LY++ NI+RMRLY P   T NALRGSNIEL+L +PN
Sbjct: 31  AVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPN 90

Query: 73  HELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAIS 132
            +L+ +A+ Q +A +WV++N+ N+ +V F+YI VGNE++  D AA F++PAMQNI+ A+S
Sbjct: 91  PDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVS 150

Query: 133 AVGLRGKIKVSTSFDT-GILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPY 192
           ++G    IKVST+ DT GI    FPPS G+F  ++   + P I FL    SPLLVN YPY
Sbjct: 151 SLG----IKVSTAIDTRGI--SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPY 210

Query: 193 FSYIDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSE 252
           FSY  N  +I LDY LF   + VV D Q  YRNLF AILD VY++LEK+GGG LEIVVSE
Sbjct: 211 FSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSE 270

Query: 253 TGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEK 312
           +GWPT  G A  VDNARTY+NNLIQ VK G+P+RP R  ETY+FAMFDEN K   PE EK
Sbjct: 271 SGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSK-QGPETEK 330

Query: 313 HWGLFSPNKQPKYHVNFN 329
            WGLF PN QPKY VNFN
Sbjct: 331 FWGLFLPNLQPKYVVNFN 341

BLAST of Spg008243 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 362.5 bits (929), Expect = 3.7e-100
Identity = 182/319 (57.05%), Postives = 233/319 (73.04%), Query Frame = 0

Query: 12  HAAAQIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIP 71
           H A QIGVCYG LG+ LPSP++V+ LYK+ NIQRMRLY P      ALRGS+IEL+L +P
Sbjct: 27  HTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVP 86

Query: 72  NHELKDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNA 131
           + +L+ +A+ Q +A  WVQ N+ ++ D V+F+YI VGNE+K       FL+ AMQNI+NA
Sbjct: 87  SSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVK--PSVGGFLLQAMQNIENA 146

Query: 132 ISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYP 191
           +S  GL  ++KVST+  T    ++ PPS+G FR +Y   L+P I FL    SPLLVN+YP
Sbjct: 147 VSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYP 206

Query: 192 YFSYIDNTAEISLDYALFRGTPVVADDQ-LVYRNLFDAILDAVYSALEKSGGGGLEIVVS 251
           YFSY+ +TA I LDYALF     V +D    Y+NLFDA LD+VY+ALEKSGGG LEIVVS
Sbjct: 207 YFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVS 266

Query: 252 ETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVE 311
           ETGWPT+  V  +V+NA+TY+NNLIQHVK G+P+RP + +ETY+FAMFDEN+K   P  E
Sbjct: 267 ETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKK--EPTYE 326

Query: 312 KHWGLFSPNKQPKYHVNFN 329
           K WGLF P++Q KY VNFN
Sbjct: 327 KFWGLFHPDRQSKYEVNFN 339

BLAST of Spg008243 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 6.3e-92
Identity = 165/316 (52.22%), Postives = 224/316 (70.89%), Query Frame = 0

Query: 17  IGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHELK 76
           +GVCYG +GN+LPS ++ I L+++NNI+R+RLY P     NALR + IE+++G+PN +L+
Sbjct: 24  VGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVPNTDLR 83

Query: 77  DMATIQGKAHSWVQHNILN-FGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVG 136
            + T    A SW+Q+N+LN +  V FKYI VGNE+    +    ++PAM+N+ +A+    
Sbjct: 84  SL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSP-SNGGDVVLPAMRNVYDALRGAN 143

Query: 137 LRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYFSYI 196
           L+ +IKVST+ D  ++  SFPPS G FRGD    +DP I FL   NS LL N+YPYFSY+
Sbjct: 144 LQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYPYFSYV 203

Query: 197 DNTAEISLDYALFRGTPVVA-DDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGWP 256
           DN  +ISL YALF    VV  D    Y+NLFDA+LD VYSA+E+SGGG L +VVSE+GWP
Sbjct: 204 DNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVVSESGWP 263

Query: 257 TDRGVAANVDNARTYINNLIQHVKV--GTPKRPRRPVETYVFAMFDENEKTTPPEVEKHW 316
           ++ G AA+ DNAR +  NL   V+   GTPKRP R VETY+FAMFDEN+K+  PE+EK++
Sbjct: 264 SNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKS--PEIEKNF 323

Query: 317 GLFSPNKQPKYHVNFN 329
           GLF PNKQPK+ + F+
Sbjct: 324 GLFFPNKQPKFPITFS 335

BLAST of Spg008243 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 256.1 bits (653), Expect = 3.7e-68
Identity = 130/323 (40.25%), Postives = 202/323 (62.54%), Query Frame = 0

Query: 16  QIGVCYGQLGNDLPSPAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHEL 75
           ++GVCYG+  +DLP+P++V+ L +++NI+ +R+Y        A   ++IELM+G+PN +L
Sbjct: 25  KVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDL 84

Query: 76  KDMATIQGKAHSWVQHNIL-NFGDVKFKYIVVGNEIKTYDH--AAQFLVPAMQNIQNAIS 135
              +  Q    +W+++++L  +   K  YI VG E     H  A+ F+VPAMQN+  A+ 
Sbjct: 85  NAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALR 144

Query: 136 AVGLRGKIKVSTSFDTGILAESFPPSKGSFRGDYLPILDPTIRFLVDNNSPLLVNVYPYF 195
            VGL  +IKVST+   GIL+ SFPPS G+F   Y   L P + FL +N SP ++++YPY+
Sbjct: 145 KVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYY 204

Query: 196 SYIDNTAEISLDYALFRGTPVVADDQ--LVYRNLFDAILDAVYSALEKSGGGGLEIVVSE 255
           +Y D+   +SLDY LF  +  V D    L+Y+N+FDA +DA+Y AL       ++I+V+E
Sbjct: 205 AYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVTE 264

Query: 256 TGWPT----DRGVAANVDNARTYINNLIQHV--KVGTPKRPRRPVETYVFAMFDENEKTT 315
           TGWPT        AA+ DNA TY +N+I+HV    GTP +P   +  Y+F++F+EN K  
Sbjct: 265 TGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRK-A 324

Query: 316 PPEVEKHWGLFSPNKQPKYHVNF 328
             + E++WGLF P++   Y ++F
Sbjct: 325 GLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022961079.12.0e-14879.39glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata][more]
KAG6590085.19.9e-14882.54Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma... [more]
XP_022987103.11.7e-14779.09probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima][more]
XP_022139185.16.4e-14782.17glucan endo-1,3-beta-glucosidase-like [Momordica charantia][more]
XP_038895085.14.5e-14077.85glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P524082.7e-11663.52Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... [more]
Q9M2M04.8e-11362.10Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q037731.6e-10862.58Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1[more]
P364018.5e-10257.68Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4... [more]
P079791.4e-9956.74Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1HCY39.7e-14979.39(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 P... [more]
A0A6J1JIH68.2e-14879.09(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=... [more]
A0A6J1CBL73.1e-14782.17(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 ... [more]
A0A1S3C9Z39.4e-13677.46(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV... [more]
A0A0A0LYQ85.1e-13476.11(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G57270.13.4e-11462.10beta-1,3-glucanase 1 [more]
AT3G57240.13.7e-10058.49beta-1,3-glucanase 3 [more]
AT3G57260.13.7e-10057.05beta-1,3-glucanase 2 [more]
AT4G16260.16.3e-9252.22Glycosyl hydrolase superfamily protein [more]
AT5G56590.13.7e-6840.25O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 15..328
e-value: 2.2E-131
score: 439.6
NoneNo IPR availablePANTHERPTHR32227:SF138GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATEDcoord: 9..325
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 17..327
e-value: 1.8E-120
score: 402.3
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 9..325
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 17..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008243.1Spg008243.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds