Spg008233 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008233
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description(1->3)-beta-glucan endohydrolase
Locationscaffold2: 18765505 .. 18766040 (-)
RNA-Seq ExpressionSpg008233
SyntenySpg008233
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCTTAAGAGGCTCCAACATTGAGCTCATGCTTGGCCTCCCCAACCCCGATCTCCAAAGTATCGCTTCAAGTCAAGCTGAAGCCAATACATGGGTTCAAAACAACATCATAAACTTTCCCAATGTCAGATTCAAGTACATTCGTTGTCCCAGCCATGCAAAACATCCAAACTGCAGTTAACAACGCTGGCTTGGGCAACCAGATCAAAGTCTCCACCGCCATTGACACCGGAGTCCTCGGAGTGTCTTTCCCTCCTTCTAGTGGCTCCTTTAGAATTGTCAACTTGTACCCTTACTTTAGCTACATCGGCAACCCCAATGCCACCATTCGTTTGGACTGCACCGTCAGTGGTGGTCACTGATGGCCAGTTTCGTTATCAAAATTTGTTTGATGCAATTCTCGATGCAGTTTACGCAGCTTTGGAGAAGGCTAGGGGAGGAAATTTGGAGATTGTGATATCCGAGAGTGGGTGGCCATCGGCAGGGGTACAGCGACATCGATTGATAATGCAAGGACTTACAACAATAATCTGA

mRNA sequence

ATGCCCTTAAGAGGCTCCAACATTGAGCTCATGCTTGGCCTCCCCAACCCCGATCTCCAAAGTATCGCTTCAAGTCAAGCTGAAGCCAATACATGGTTAACAACGCTGGCTTGGGCAACCAGATCAAAGTCTCCACCGCCATTGACACCGGAGTCCTCGGAGTGTCTTTCCCTCCTTCTAGTGGCTCCTTTAGAATTGTCAACTTGTACCCTTACTTTAGCTACATCGGCAACCCCAATGCCACCATTCGTTTGGACTGCACCGTCAGTGGTGGTCACTGATGGCCAGTTTCGTTATCAAAATTTGTTTGATGCAATTCTCGATGCAGTTTACGCAGCTTTGGAGAAGGCTAGGGGAGGAAATTTGGAGATTGTGATATCCGAGAGTGGGTGGCCATCGGCAGGGGTACAGCGACATCGATTGATAATGCAAGGACTTACAACAATAATCTGA

Coding sequence (CDS)

ATGCCCTTAAGAGGCTCCAACATTGAGCTCATGCTTGGCCTCCCCAACCCCGATCTCCAAAGTATCGCTTCAAGTCAAGCTGAAGCCAATACATGGTTAACAACGCTGGCTTGGGCAACCAGATCAAAGTCTCCACCGCCATTGACACCGGAGTCCTCGGAGTGTCTTTCCCTCCTTCTAGTGGCTCCTTTAGAATTGTCAACTTGTACCCTTACTTTAGCTACATCGGCAACCCCAATGCCACCATTCGTTTGGACTGCACCGTCAGTGGTGGTCACTGATGGCCAGTTTCGTTATCAAAATTTGTTTGATGCAATTCTCGATGCAGTTTACGCAGCTTTGGAGAAGGCTAGGGGAGGAAATTTGGAGATTGTGATATCCGAGAGTGGGTGGCCATCGGCAGGGGTACAGCGACATCGATTGATAATGCAAGGACTTACAACAATAATCTGA

Protein sequence

MPLRGSNIELMLGLPNPDLQSIASSQAEANTWLTTLAWATRSKSPPPLTPESSECLSLLLVAPLELSTCTLTLATSATPMPPFVWTAPSVVVTDGQFRYQNLFDAILDAVYAALEKARGGNLEIVISESGWPSAGVQRHRLIMQGLTTII
Homology
BLAST of Spg008233 vs. NCBI nr
Match: KAA0038295.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 112.1 bits (279), Expect = 4.3e-21
Identity = 87/200 (43.50%), Postives = 99/200 (49.50%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGS IELMLGLPNPDLQ IASSQAEAN W+             +        P +  +S
Sbjct: 36  LRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNEVQPSSSAAS 95

Query: 63  ECLSLL-----------LVAPLELSTC--TLTLATSATP---------MP---------- 122
             +  +           LV  +++ST   T  LA S  P         MP          
Sbjct: 96  FVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPFMSSVIRFLK 155

Query: 123 -----------PF---------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 136
                      P+               ++TAPS VV DGQFRYQNLFDAILDAVYAALE
Sbjct: 156 DNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAILDAVYAALE 215

BLAST of Spg008233 vs. NCBI nr
Match: XP_008443726.1 (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 112.1 bits (279), Expect = 4.3e-21
Identity = 87/200 (43.50%), Postives = 99/200 (49.50%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGS IELMLGLPNPDLQ IASSQAEAN W+             +        P +  +S
Sbjct: 70  LRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNEVQPSSSAAS 129

Query: 63  ECLSLL-----------LVAPLELSTC--TLTLATSATP---------MP---------- 122
             +  +           LV  +++ST   T  LA S  P         MP          
Sbjct: 130 FVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPFMSSVIRFLK 189

Query: 123 -----------PF---------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 136
                      P+               ++TAPS VV DGQFRYQNLFDAILDAVYAALE
Sbjct: 190 DNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAILDAVYAALE 249

BLAST of Spg008233 vs. NCBI nr
Match: CAB4286634.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 110.9 bits (276), Expect = 9.5e-21
Identity = 75/142 (52.82%), Postives = 89/142 (62.68%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL--TTLAWATRSKSPPPLTPESSECLSLLL 62
           LRGSNIELMLG+PN +LQS+ASSQA ANTW+      +   + S   L  + S  +   +
Sbjct: 83  LRGSNIELMLGVPNDNLQSLASSQANANTWVQNNVRNYGNNAISSAGLGIKVSTAIDTGV 142

Query: 63  VAPL------ELSTCTLTLATSATPMPPFVWTA-PSVVVTDGQFRYQNLFDAILDAVYAA 122
           +         E  +    L     P+P     A PSVVV DGQ  Y+NLFDAILDAVYAA
Sbjct: 143 LGNSFPPSNGEFKSEYGALLNPHHPLPSEQQIAPPSVVVQDGQRGYRNLFDAILDAVYAA 202

Query: 123 LEKARGGNLEIVISESGWPSAG 136
           LEKA GG+LEIVISESGWPSAG
Sbjct: 203 LEKAGGGSLEIVISESGWPSAG 224

BLAST of Spg008233 vs. NCBI nr
Match: TYK19849.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 110.5 bits (275), Expect = 1.2e-20
Identity = 86/200 (43.00%), Postives = 98/200 (49.00%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGS IELMLGLPNPDLQ IASSQAEAN W+             +        P +  +S
Sbjct: 36  LRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNEVQPSSSAAS 95

Query: 63  ECLSLL-----------LVAPLELSTC--TLTLATSATP---------MP---------- 122
             +  +           L   +++ST   T  LA S  P         MP          
Sbjct: 96  FVVPAMRNIQTALNNAGLANRIKVSTAIDTSILAMSYPPSSGTIKNEDMPFMSSVIRFLK 155

Query: 123 -----------PF---------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 136
                      P+               ++TAPS VV DGQFRYQNLFDAILDAVYAALE
Sbjct: 156 DNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAILDAVYAALE 215

BLAST of Spg008233 vs. NCBI nr
Match: THG22084.1 (hypothetical protein TEA_027928 [Camellia sinensis var. sinensis])

HSP 1 Score: 107.5 bits (267), Expect = 1.0e-19
Identity = 73/164 (44.51%), Postives = 91/164 (55.49%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL--------------------------TTL 62
           LRGSNIELMLG+PN DLQ +AS+Q EAN W+                           T 
Sbjct: 68  LRGSNIELMLGVPNTDLQRLASNQTEANIWVQNNVKNYGNVKFRYIAVGNEVDPSNPNTA 127

Query: 63  AWATRSKSPPPLTPESSECLSLLL--VAPLELSTCTLTLATSAT---PMPPFVWTAPSVV 122
            +   +   P L P     +  L    APL L+        + T    +   ++TAPSVV
Sbjct: 128 PFLLPAMRKPELGPFLDPIIGFLANNGAPLLLNLYPYFSYANNTIDISLDYALFTAPSVV 187

Query: 123 VTDGQFRYQNLFDAILDAVYAALEKARGGNLEIVISESGWPSAG 136
           VTDG   Y+NLFDA+LDAVY+ALEKA GG+LEIV+SESGWP+AG
Sbjct: 188 VTDGSLGYRNLFDAMLDAVYSALEKAGGGSLEIVVSESGWPTAG 231

BLAST of Spg008233 vs. ExPASy Swiss-Prot
Match: F4J270 (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN=BG3 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.9e-19
Identity = 72/195 (36.92%), Postives = 94/195 (48.21%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGSNIEL+L +PNPDLQ +ASSQAEA+TW+          T  + +      P    +S
Sbjct: 77  LRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAAS 136

Query: 63  ECLSLLLVAPLELSTCTLTLAT-------------SATPMPPF----------------- 122
             L  +      +S+  + ++T             S T  P F                 
Sbjct: 137 FVLPAMQNIERAVSSLGIKVSTAIDTRGISGFPPSSGTFTPEFRSFIAPVISFLSSKQSP 196

Query: 123 -----------------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALEKARGG 136
                                  ++TAPS VV DGQ +Y+NLF AILD VYA+LEKA GG
Sbjct: 197 LLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGG 256

BLAST of Spg008233 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.9e-17
Identity = 72/203 (35.47%), Postives = 96/203 (47.29%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGSNIELMLG+PNPDL+++A+SQA A+TW+             +        PL  E+S
Sbjct: 68  LRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLN-ENS 127

Query: 63  ECLSLLLVA---------------PLELSTCTLTLATSATPMP----------------- 122
           + + +LL A                +++ST   T  T+ T  P                 
Sbjct: 128 KYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEPII 187

Query: 123 ---------------PF--------------VWTAPSVVVTDGQFRYQNLFDAILDAVYA 136
                          P+              ++T+  VVV D    Y+NLFDAILDA Y+
Sbjct: 188 NFLVTNRAPLLVNLYPYFAIANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDATYS 247

BLAST of Spg008233 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-16
Identity = 73/199 (36.68%), Postives = 94/199 (47.24%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWLTT------------LAWATRSKSPP---- 62
           LRGSNI+L+LG+PN +LQ IA SQA AN W+              +A     K       
Sbjct: 82  LRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSFAQ 141

Query: 63  ---PLTPESSECLSLL-LVAPLELSTCTLT----------------------------LA 122
              P      E +SL  L   +++ST   T                            L 
Sbjct: 142 FLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLV 201

Query: 123 TSATPM-----PPF--------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 135
           +  +P+     P F              ++TAPSVVV DG F Y+NLFDA+LD VYAALE
Sbjct: 202 SHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALE 261

BLAST of Spg008233 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 7.3e-16
Identity = 69/204 (33.82%), Postives = 93/204 (45.59%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL----------TTLAWATRSKSPPPLTPES 62
           LRGSNIE+MLG+PN DLQ+IA++ + AN W+              +        P+T  S
Sbjct: 75  LRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTS 134

Query: 63  SECLSLLLV----------APLE-----LSTCTLTLATSATP------------------ 122
           S    LL            A L+      S+  +TL  ++ P                  
Sbjct: 135 SLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPII 194

Query: 123 ----------------------------MPPFVWTAPSVVVTDGQFRYQNLFDAILDAVY 136
                                       +P  ++TAP+VVV DG   Y+NLFDA+ DAVY
Sbjct: 195 GFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAVY 254

BLAST of Spg008233 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 70/204 (34.31%), Postives = 95/204 (46.57%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL--TTLAWATRSKSP--------------- 62
           LRGS+IEL+L +PN DLQS+AS  + A TW+    + +A+  K                 
Sbjct: 72  LRGSDIELILDVPNTDLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNA 131

Query: 63  -------PPLTPESSECLSLLLVAPLELSTCTLT-------------------------- 122
                  P +    S   S  L   +++ST T +                          
Sbjct: 132 QYAQYVLPAMKNVQSAITSAGLQDQIKVSTATFSAVLGKSYPPSEGSFSDDVSSFINPII 191

Query: 123 --LATSATPM-----PPF--------------VWTAPSVVVTDGQFRYQNLFDAILDAVY 136
             LA + +P+     P F              ++TA  VVV DG ++YQNLFDA+LDA+Y
Sbjct: 192 SFLAENGSPLLANIYPYFSYTGDTQNIRLDYALFTASGVVVQDGSYQYQNLFDALLDALY 251

BLAST of Spg008233 vs. ExPASy TrEMBL
Match: A0A5A7TA86 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G001600 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.1e-21
Identity = 87/200 (43.50%), Postives = 99/200 (49.50%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGS IELMLGLPNPDLQ IASSQAEAN W+             +        P +  +S
Sbjct: 36  LRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNEVQPSSSAAS 95

Query: 63  ECLSLL-----------LVAPLELSTC--TLTLATSATP---------MP---------- 122
             +  +           LV  +++ST   T  LA S  P         MP          
Sbjct: 96  FVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPFMSSVIRFLK 155

Query: 123 -----------PF---------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 136
                      P+               ++TAPS VV DGQFRYQNLFDAILDAVYAALE
Sbjct: 156 DNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAILDAVYAALE 215

BLAST of Spg008233 vs. ExPASy TrEMBL
Match: A0A1S3B9H8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.1e-21
Identity = 87/200 (43.50%), Postives = 99/200 (49.50%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGS IELMLGLPNPDLQ IASSQAEAN W+             +        P +  +S
Sbjct: 70  LRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNEVQPSSSAAS 129

Query: 63  ECLSLL-----------LVAPLELSTC--TLTLATSATP---------MP---------- 122
             +  +           LV  +++ST   T  LA S  P         MP          
Sbjct: 130 FVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPFMSSVIRFLK 189

Query: 123 -----------PF---------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 136
                      P+               ++TAPS VV DGQFRYQNLFDAILDAVYAALE
Sbjct: 190 DNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAILDAVYAALE 249

BLAST of Spg008233 vs. ExPASy TrEMBL
Match: A0A6J5VFH2 ((1->3)-beta-glucan endohydrolase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS44172 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.6e-21
Identity = 75/142 (52.82%), Postives = 89/142 (62.68%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL--TTLAWATRSKSPPPLTPESSECLSLLL 62
           LRGSNIELMLG+PN +LQS+ASSQA ANTW+      +   + S   L  + S  +   +
Sbjct: 83  LRGSNIELMLGVPNDNLQSLASSQANANTWVQNNVRNYGNNAISSAGLGIKVSTAIDTGV 142

Query: 63  VAPL------ELSTCTLTLATSATPMPPFVWTA-PSVVVTDGQFRYQNLFDAILDAVYAA 122
           +         E  +    L     P+P     A PSVVV DGQ  Y+NLFDAILDAVYAA
Sbjct: 143 LGNSFPPSNGEFKSEYGALLNPHHPLPSEQQIAPPSVVVQDGQRGYRNLFDAILDAVYAA 202

Query: 123 LEKARGGNLEIVISESGWPSAG 136
           LEKA GG+LEIVISESGWPSAG
Sbjct: 203 LEKAGGGSLEIVISESGWPSAG 224

BLAST of Spg008233 vs. ExPASy TrEMBL
Match: A0A5D3D8R0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold811G00600 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 6.0e-21
Identity = 86/200 (43.00%), Postives = 98/200 (49.00%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGS IELMLGLPNPDLQ IASSQAEAN W+             +        P +  +S
Sbjct: 36  LRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNEVQPSSSAAS 95

Query: 63  ECLSLL-----------LVAPLELSTC--TLTLATSATP---------MP---------- 122
             +  +           L   +++ST   T  LA S  P         MP          
Sbjct: 96  FVVPAMRNIQTALNNAGLANRIKVSTAIDTSILAMSYPPSSGTIKNEDMPFMSSVIRFLK 155

Query: 123 -----------PF---------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALE 136
                      P+               ++TAPS VV DGQFRYQNLFDAILDAVYAALE
Sbjct: 156 DNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAILDAVYAALE 215

BLAST of Spg008233 vs. ExPASy TrEMBL
Match: A0A4S4EZL1 (Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_027928 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 5.1e-20
Identity = 73/164 (44.51%), Postives = 91/164 (55.49%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL--------------------------TTL 62
           LRGSNIELMLG+PN DLQ +AS+Q EAN W+                           T 
Sbjct: 68  LRGSNIELMLGVPNTDLQRLASNQTEANIWVQNNVKNYGNVKFRYIAVGNEVDPSNPNTA 127

Query: 63  AWATRSKSPPPLTPESSECLSLLL--VAPLELSTCTLTLATSAT---PMPPFVWTAPSVV 122
            +   +   P L P     +  L    APL L+        + T    +   ++TAPSVV
Sbjct: 128 PFLLPAMRKPELGPFLDPIIGFLANNGAPLLLNLYPYFSYANNTIDISLDYALFTAPSVV 187

Query: 123 VTDGQFRYQNLFDAILDAVYAALEKARGGNLEIVISESGWPSAG 136
           VTDG   Y+NLFDA+LDAVY+ALEKA GG+LEIV+SESGWP+AG
Sbjct: 188 VTDGSLGYRNLFDAMLDAVYSALEKAGGGSLEIVVSESGWPTAG 231

BLAST of Spg008233 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 97.1 bits (240), Expect = 1.3e-20
Identity = 72/195 (36.92%), Postives = 94/195 (48.21%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL---------TTLAWATRSKSPPPLTPESS 62
           LRGSNIEL+L +PNPDLQ +ASSQAEA+TW+          T  + +      P    +S
Sbjct: 77  LRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAAS 136

Query: 63  ECLSLLLVAPLELSTCTLTLAT-------------SATPMPPF----------------- 122
             L  +      +S+  + ++T             S T  P F                 
Sbjct: 137 FVLPAMQNIERAVSSLGIKVSTAIDTRGISGFPPSSGTFTPEFRSFIAPVISFLSSKQSP 196

Query: 123 -----------------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALEKARGG 136
                                  ++TAPS VV DGQ +Y+NLF AILD VYA+LEKA GG
Sbjct: 197 LLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGG 256

BLAST of Spg008233 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 74.3 bits (181), Expect = 9.2e-14
Identity = 61/202 (30.20%), Postives = 90/202 (44.55%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWLTT---------------------LAWATR 62
           LRGSNIE+ LGLPN  LQS+ASSQ++AN W+ T                     ++ +  
Sbjct: 69  LRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYA 128

Query: 63  SKSPPPLTPESSECLSLLLVAPLELSTCTLTLATSATPMPP------------------- 122
               P +       L+  L   +++ST ++ +       PP                   
Sbjct: 129 QFLVPAMENIDRAVLAAGLGGRIKVST-SVDMGVLRESYPPSKGSFRGDVMVVMEPIIRF 188

Query: 123 -----------------------------FVWTAPSVVVTDGQFRYQNLFDAILDAVYAA 136
                                         ++TAPS +V+D    YQNLFDA+LDA+Y+A
Sbjct: 189 LVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSA 248

BLAST of Spg008233 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 9.2e-14
Identity = 33/52 (63.46%), Postives = 44/52 (84.62%), Query Frame = 0

Query: 84  VWTAPSVVVTDGQFRYQNLFDAILDAVYAALEKARGGNLEIVISESGWPSAG 136
           ++T+PSVVV DG   YQNLFDA+LD VY+A+E++ GG+L +V+SESGWPS G
Sbjct: 213 LFTSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVVSESGWPSNG 264

BLAST of Spg008233 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-12
Identity = 63/197 (31.98%), Postives = 85/197 (43.15%), Query Frame = 0

Query: 3   LRGSNIELMLGLPNPDLQSIASSQAEANTWL----------------------------- 62
           LRGS+IEL+L +P+ DL+ +ASSQ EA+ W+                             
Sbjct: 74  LRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPSVGGF 133

Query: 63  ----------------------TTLAW-ATRSKSPPPLTPESSECLSLLLVAPLELSTCT 122
                                 T +A   T   SPP       E  S L      L++  
Sbjct: 134 LLQAMQNIENAVSGAGLEVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQ 193

Query: 123 LTLATSATPMPPF------------VWTAPSVVVTDGQFRYQNLFDAILDAVYAALEKAR 136
             L  +  P   +            ++TA S V  D  + YQNLFDA LD+VYAALEK+ 
Sbjct: 194 SPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSG 253

BLAST of Spg008233 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-09
Identity = 29/64 (45.31%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 89  SVVVTDGQFRYQNLFDAILDAVYAALEKARGGNLEIVISESGWPSAG------VQRHRLI 147
           S VVTDG+++Y N+FDA LDA   ALEK   G++++ ++E+GWP+ G      V+  R  
Sbjct: 217 SPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWPTRGNDPYTSVENARAY 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0038295.14.3e-2143.50glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa][more]
XP_008443726.14.3e-2143.50PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo][more]
CAB4286634.19.5e-2152.82unnamed protein product [Prunus armeniaca][more]
TYK19849.11.2e-2043.00glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo var. makuwa][more]
THG22084.11.0e-1944.51hypothetical protein TEA_027928 [Camellia sinensis var. sinensis][more]
Match NameE-valueIdentityDescription
F4J2701.9e-1936.92Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN... [more]
P364013.9e-1735.47Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4... [more]
P524081.5e-1636.68Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... [more]
P079797.3e-1633.82Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3[more]
A7PQW31.2e-1534.31Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120... [more]
Match NameE-valueIdentityDescription
A0A5A7TA862.1e-2143.50(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3B9H82.1e-2143.50(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV... [more]
A0A6J5VFH24.6e-2152.82(1->3)-beta-glucan endohydrolase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS441... [more]
A0A5D3D8R06.0e-2143.00(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A4S4EZL15.1e-2044.51Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_0279... [more]
Match NameE-valueIdentityDescription
AT3G57240.11.3e-2036.92beta-1,3-glucanase 3 [more]
AT3G57270.19.2e-1430.20beta-1,3-glucanase 1 [more]
AT4G16260.19.2e-1463.46Glycosyl hydrolase superfamily protein [more]
AT3G57260.11.3e-1231.98beta-1,3-glucanase 2 [more]
AT1G77780.11.8e-0945.31Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 79..136
e-value: 6.5E-16
score: 58.8
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 122..135
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..43
e-value: 6.8E-6
score: 27.3
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 68..148
e-value: 1.5E-17
score: 65.6
NoneNo IPR availablePANTHERPTHR32227:SF390GLUCAN ENDO-1,3-BETA-GLUCOSIDASE, ACIDIC-LIKEcoord: 84..138
NoneNo IPR availablePANTHERPTHR32227:SF390GLUCAN ENDO-1,3-BETA-GLUCOSIDASE, ACIDIC-LIKEcoord: 3..34
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 84..138
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 3..34
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 3..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008233.1Spg008233.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds