Spg008194 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008194
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptiontRNA (adenine(58)-N(1))-methyltransferase
Locationscaffold2: 19089604 .. 19091760 (-)
RNA-Seq ExpressionSpg008194
SyntenySpg008194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGGTCTTTCTTCTTCCATTAGGGCTGGATTTCACGATTTTGAACATAGCAGAGAGAGAGGGAAAAAACCCAAAAACAGAGAGGAAGAAAAACGCAGCCGCCGGTCACTGGGTTTCGTTGGTCTCGGTTCAGAGGTCCGTTCGGCGCCGGTTGTGGGTCTCGGGTTGTACGGCAGAAGTGGGTCTCGATTAGAGACGTCGTGGGTCCCTGTTCAGGCGCCGGTGTGGGCCTCGGAGAAGCGACTTCGCGTGTGTTTCGGTTGAGTGCAGCTGTGGGTCTCGGTTTAGGGACTGTTTTGCTCCGGTTTGAAGCTCGTTTCTTCTCAGTTGTGGGTCTCGGTATAGGAGGCTGAATCGTGTTGTGGGTGTTTCGTTGGAAGCTCAAATCGGCCAAGATTTGAGGTATTTCACTGGTTGAATTTAATACCATTTATACTCTGTATTTGTGACTTGATGCTCGTTGATAGAAGTCTCATATAAGCTGGTTCTCAATCTGGAGGTATATCGAAAGGTTAAATCACTGTATATCTTTGTGTTCTTTCTCTCTTGGTTGTGCTTATTTGTTGCTGCTCTTTTCCAATGGAAAATTTGTAGAACTGTCCAATTAGATTCTTTGAGCGAGAATTGAGTGTGTAGAGCGATTGACAACTGAAGTTTTTAAAAGACATTTATTCCAGATAGTAGTTTCGATTTTAACTACTTATTGTCCAAAATGTGTTGCTGTTATTGTGTATCTTTGTTTTATTTATTTGTTTTTTCCCCTAAATTTCTACAGAAGTTGGAATTCTTGATTCTTTATAAACTTTTGAAGACCAAGGATGTTGCCAACTGATGAGTCGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTCTATGAGAGACATGACATAATGAAGGCTGTGAAAGTGTGTGAAAGTTCAACATTTCAGAATAGGTTTGGCGTGTTTAAACATTCGGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTCAGCCACAGGACACAGATTCTATACATCGCAGACATCAGCTTCGTAATTATGTTCTTGGAAATAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACGGGGAGTGGATCTTTGACAACGTCGTTAGCGAGGGCTGTAGCGCCTACCGGACACGTCCATACGTTTGATTTTCATGAACAGAGGGCTGCCTCGGCGAGGTAGGATCGTAGATCTAATGTGTGTTTTCAAGTTACCCTGCTGAGTAATTGTTCTATAATGGTTTTCCATTTTTAAACAGATCTTGAGCCTGTTTAAGTAGTACTTATTGCTTGTTTCCCTCTTCTTTCAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTGACTGTGAGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGTTGAAACAAGATGGCGTGTTATGCTCCTTCTCCCCGTGTATCGAGCAAGTTCAACGCTCGGCAGAAACTCTTCGATCTAAGTTTACAGGTTCTAAATGACCCTATTGTCATTTAACTTCATTGCTGAGATATGTTGAATTGTAGTGTTCACTTTTGGTCCTCTTTTGTGTAGATATAAGGACATTTGAGGTATTGCTTCGAACATATGAAGTTCGGGAAGGTAAACCAGAGAGTAGCCAGGTTGATGGAGGTGGCTCTGTTGGGATTCCTCCGTGCAAGAGGCGGCTGAGATCGAATGAAGCAAACGACATGAACGATGATTCGAGTACGACGGTCATGGTCAGGCCATGTAGTGAGGCCAGAGGGCACACTGGTTATCTAACATTTGCTAGACTTAGGTGCATCTCATAACACTAATATAGTTTATGAATTCTTTAACAAGGAAATTTGAAGTTTTGTATTCTTTTTCATCACTCTGGAACCTTGATTCTTATTTAAATTATTGAATGTTCAATAATTTTATTCCTGATATCATCACTCTTCATCATCTTGGCTAATTTCATGAGTTTTGTACTTTTGACTTTGCCAGACACCCA

mRNA sequence

ATGGAATTTGGTCTTTCTTCTTCCATTAGGGCTGGATTTCACGATTTTGAACATAGCAGAGAGAGAGGGAAAAAACCCAAAAACAGAGAGGAAGAAAAACGCAGCCGCCGGTCACTGGGTTTCGTTGGTCTCGGTTCAGAGGTCCGTTCGGCGCCGGTTGTGGGTCTCGGGTTGTACGGCAGAAGTGGTTGTGGGTCTCGGTATAGGAGGCTGAATCGTGTTGTGGGTGTTTCGTTGGAAGCTCAAATCGGCCAAGATTTGAGACCAAGGATGTTGCCAACTGATGAGTCGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTCTATGAGAGACATGACATAATGAAGGCTGTGAAAGTGTGTGAAAGTTCAACATTTCAGAATAGGTTTGGCGTGTTTAAACATTCGGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTCAGCCACAGGACACAGATTCTATACATCGCAGACATCAGCTTCGTAATTATGTTCTTGGAAATAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACGGGGAGTGGATCTTTGACAACGTCGTTAGCGAGGGCTGTAGCGCCTACCGGACACGTCCATACGTTTGATTTTCATGAACAGAGGGCTGCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTGACTGTGAGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGTTGAAACAAGATGGCGTGTTATGCTCCTTCTCCCCGTGTATCGAGCAAGTTCAACGCTCGGCAGAAACTCTTCGATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGAACATATGAAGTTCGGGAAGGTAAACCAGAGAGTAGCCAGGTTGATGGAGGTGGCTCTGTTGGGATTCCTCCGTGCAAGAGGCGGCTGAGATCGAATGAAGCAAACGACATGAACGATGATTCGAGTACGACGGTCATGGTCAGGCCATGTAGTGAGGCCAGAGGGCACACTGGTTATCTAACATTTGCTAGACTTAGGTGCATCTCATAA

Coding sequence (CDS)

ATGGAATTTGGTCTTTCTTCTTCCATTAGGGCTGGATTTCACGATTTTGAACATAGCAGAGAGAGAGGGAAAAAACCCAAAAACAGAGAGGAAGAAAAACGCAGCCGCCGGTCACTGGGTTTCGTTGGTCTCGGTTCAGAGGTCCGTTCGGCGCCGGTTGTGGGTCTCGGGTTGTACGGCAGAAGTGGTTGTGGGTCTCGGTATAGGAGGCTGAATCGTGTTGTGGGTGTTTCGTTGGAAGCTCAAATCGGCCAAGATTTGAGACCAAGGATGTTGCCAACTGATGAGTCGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTCTATGAGAGACATGACATAATGAAGGCTGTGAAAGTGTGTGAAAGTTCAACATTTCAGAATAGGTTTGGCGTGTTTAAACATTCGGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTCAGCCACAGGACACAGATTCTATACATCGCAGACATCAGCTTCGTAATTATGTTCTTGGAAATAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACGGGGAGTGGATCTTTGACAACGTCGTTAGCGAGGGCTGTAGCGCCTACCGGACACGTCCATACGTTTGATTTTCATGAACAGAGGGCTGCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACCAGCTTAGTGACTGTGAGAGTTAGAGATATTCAAGGTCAGGGGTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGTTGAAACAAGATGGCGTGTTATGCTCCTTCTCCCCGTGTATCGAGCAAGTTCAACGCTCGGCAGAAACTCTTCGATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGAACATATGAAGTTCGGGAAGGTAAACCAGAGAGTAGCCAGGTTGATGGAGGTGGCTCTGTTGGGATTCCTCCGTGCAAGAGGCGGCTGAGATCGAATGAAGCAAACGACATGAACGATGATTCGAGTACGACGGTCATGGTCAGGCCATGTAGTGAGGCCAGAGGGCACACTGGTTATCTAACATTTGCTAGACTTAGGTGCATCTCATAA

Protein sequence

MEFGLSSSIRAGFHDFEHSRERGKKPKNREEEKRSRRSLGFVGLGSEVRSAPVVGLGLYGRSGCGSRYRRLNRVVGVSLEAQIGQDLRPRMLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFARLRCIS
Homology
[Close]
BLAST of Spg008194 vs. NCBI nr
Match: XP_038894928.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894929.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894930.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894931.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894932.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894933.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894934.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894935.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894936.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894937.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida])

HSP 1 Score: 591.7 bits (1524), Expect = 4.9e-165
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTDE KKISFTRSIRDGDLVIVYERHDIMKAVKVCE ST QNRFGVFKH+DWIGKPFG
Sbjct: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCLVLESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLTSLVTV VRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEV+EG+ ES+QVDGGGSV +PPCKRRLRS E++DMNDDSST VMVRPCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 310

[Close]
BLAST of Spg008194 vs. NCBI nr
Match: XP_022987109.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 589.3 bits (1518), Expect = 2.4e-164
Identity = 291/310 (93.87%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCESSTFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Spg008194 vs. NCBI nr
Match: KAE8653334.1 (hypothetical protein Csa_023207, partial [Cucumis sativus])

HSP 1 Score: 588.6 bits (1516), Expect = 4.1e-164
Identity = 288/311 (92.60%), Postives = 302/311 (97.11%), Query Frame = 0 

Query: 90  RMLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPF 149
           RMLPTDE K ISFTRSIRDGDLVIVYERHDIMKAVKVC+ ST QNRFGVFKHSDWIGKPF
Sbjct: 65  RMLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPF 124

Query: 150 GSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGS 209
           GSK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGS
Sbjct: 125 GSKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGS 184

Query: 210 GSLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFV 269
           GSLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLTSLVTV VRDIQGQGFPEEFV
Sbjct: 185 GSLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFV 244

Query: 270 GLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVL 329
           GLADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVL
Sbjct: 245 GLADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVL 304

Query: 330 LRTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTG 389
           LRTYEV+EG+ ES+QVDGGGSVG+PPCKRRLRS E++DM+++SSTTVMVRPCSEARGHTG
Sbjct: 305 LRTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTG 364

Query: 390 YLTFARLRCIS 401
           YLTFARLRC+S
Sbjct: 365 YLTFARLRCLS 375

[Close]
BLAST of Spg008194 vs. NCBI nr
Match: KAG7023747.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588.2 bits (1515), Expect = 5.4e-164
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCE+STFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAE MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAESMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EANDMN DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDMNGDSS-TVMVRPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Spg008194 vs. NCBI nr
Match: XP_022158112.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica charantia] >XP_022158113.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica charantia])

HSP 1 Score: 588.2 bits (1515), Expect = 5.4e-164
Identity = 289/310 (93.23%), Postives = 299/310 (96.45%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+SKKISFTRSI DGDLVIVYERHDIMKAVKVCE ST QNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLT+LVTV VRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVRE K E SQVDGGGSVG+PPCKRRLRSN+ANDM++D STTVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTF+RLRCIS
Sbjct: 301 LTFSRLRCIS 310

[Close]
BLAST of Spg008194 vs. ExPASy Swiss-Prot
Match: Q80XC2 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus OX=10090 GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.5e-65
Identity = 140/298 (46.98%), Postives = 189/298 (63.42%), Query Frame = 0 

Query: 100 ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 159
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV+ ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 160 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 219
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 220 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 279
           +VAPTGH+HT +FH+QRA  ARE+F++  L+  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 280 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 339
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 340 EGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFA 395
                        +V +P       + E N M  D+S      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGANNLETN-MGSDASPFRSGTPMKETVGHTGYLTFA 285

[Close]
BLAST of Spg008194 vs. ExPASy Swiss-Prot
Match: Q96FX7 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens OX=9606 GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 7.8e-65
Identity = 139/298 (46.64%), Postives = 186/298 (62.42%), Query Frame = 0 

Query: 100 ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 159
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 160 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 219
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 220 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 279
            +APTGH+HT +FH+QRA  ARE+F++  +   VTVR +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 280 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 339
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L ++ F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 340 EGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFA 395
                        +V +PP    L +        D+S      P  EA GHTGYLTFA
Sbjct: 244 -------------TVSLPP--PDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFA 284

[Close]
BLAST of Spg008194 vs. ExPASy Swiss-Prot
Match: Q6AY46 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus OX=10116 GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.5e-63
Identity = 137/301 (45.51%), Postives = 187/301 (62.13%), Query Frame = 0 

Query: 100 ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 159
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV  ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 160 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 219
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 220 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 279
           ++APTGH+HT +FH+QRA  ARE+F++  ++  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 280 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVR 339
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 340 EGK---PESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTF 395
                 P+    D  G+VG                  D++      P  E  GHTGYLTF
Sbjct: 244 TVSLPLPDLGADDLEGNVG-----------------SDATPFRSGTPMKETVGHTGYLTF 285

[Close]
BLAST of Spg008194 vs. ExPASy Swiss-Prot
Match: A6H791 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus OX=9913 GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.2e-60
Identity = 135/298 (45.30%), Postives = 181/298 (60.74%), Query Frame = 0 

Query: 100 ISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHS-DWIGKPFGSKVLSNKG 159
           +++   I++GD  I+   H  M AV+V   +  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 160 GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLAR 219
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 220 AVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVGLADSVFLD 279
            +APTGH+HT +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 280 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLRS-KFTDIRTFEVLLRTYEVR 339
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  F+++ T EVL + Y VR
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVR 243

Query: 340 EGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGYLTFA 395
                        S+ +P    R           D++      P  E  GHTGYLTFA
Sbjct: 244 T-----------VSLPVPDLGAR--------PGPDAAPFRSGTPMKETVGHTGYLTFA 280

[Close]
BLAST of Spg008194 vs. ExPASy Swiss-Prot
Match: Q86JJ0 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum OX=44689 GN=trmt61a PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.2e-60
Identity = 129/306 (42.16%), Postives = 190/306 (62.09%), Query Frame = 0 

Query: 106 IRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFGSKVLSNKG-GFVYLL 165
           I++GD V++Y   D M  + +  ++ + ++FG ++H + IGK +GSK+ S+ G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 166 APTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSGSLTTSLARAVAPTG 225
           A TPELW++ L HRTQIL+  DIS +I  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 226 HVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQG-QGFPEEFVGL-ADSVFLDLPQP 285
           H+ TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 286 WLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLR-SKFTDIRTFEVLLRTYEVREGKPE 345
           W AI +A  ++    +LCSFSPCIEQVQ +   L  SKF +I+T EVL+RT++ R  + E
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 346 SSQVDGGGSVGIPPCKRRLRSNEANDMNDD------------SSTTVMVRPCSEARGHTG 396
              +        P       +N  N++ ++                ++ +P +EARGHTG
Sbjct: 248 ELNLTN------PYIDNNNNNNNNNNIEENRGKFEIGGIEGLKKDKLLSKPFTEARGHTG 307

[Close]
BLAST of Spg008194 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 1.1e-165
Identity = 294/323 (91.02%), Postives = 309/323 (95.67%), Query Frame = 0 

Query: 78  SLEAQIGQDLRPRMLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFG 137
           S+EA I   LR RMLPTDE K ISFTRSIRDGDLVIVYERHDIMKAVKVC+ ST QNRFG
Sbjct: 384 SIEAGILDSLR-RMLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFG 443

Query: 138 VFKHSDWIGKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIV 197
           VFKHSDWIGKPFGSK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIV
Sbjct: 444 VFKHSDWIGKPFGSKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIV 503

Query: 198 PGCLVLESGTGSGSLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVR 257
           PGCLVLESGTGSGSLTTSLARAVAPTGHV+TFDFHEQRA+SAREDFEKTGLTSLVTV VR
Sbjct: 504 PGCLVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVR 563

Query: 258 DIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLR 317
           DIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETLR
Sbjct: 564 DIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLR 623

Query: 318 SKFTDIRTFEVLLRTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVM 377
           SKFTDIRTFEVLLRTYEV+EG+ ES+QVDGGGSVG+PPCKRRLRS E++DM+++SSTTVM
Sbjct: 624 SKFTDIRTFEVLLRTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVM 683

Query: 378 VRPCSEARGHTGYLTFARLRCIS 401
           VRPCSEARGHTGYLTFARLRC+S
Sbjct: 684 VRPCSEARGHTGYLTFARLRCLS 705

[Close]
BLAST of Spg008194 vs. ExPASy TrEMBL
Match: A0A6J1JHY4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.2e-164
Identity = 291/310 (93.87%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCESSTFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Spg008194 vs. ExPASy TrEMBL
Match: A0A6J1DWC1 (tRNA (adenine(58)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024670 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.6e-164
Identity = 289/310 (93.23%), Postives = 299/310 (96.45%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+SKKISFTRSI DGDLVIVYERHDIMKAVKVCE ST QNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDDSKKISFTRSISDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLS+KGGFVYLLAPTPELWTLVLSHRTQILYIADISF+IMFLEIVPGCLVLESGTGSG
Sbjct: 61  SKVLSHKGGFVYLLAPTPELWTLVLSHRTQILYIADISFIIMFLEIVPGCLVLESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLT+LVTV VRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTNLVTVGVRDIQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAERMLK DGVLCSFSPCIEQVQRSAE LRS FTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKPDGVLCSFSPCIEQVQRSAEALRSSFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVRE K E SQVDGGGSVG+PPCKRRLRSN+ANDM++D STTVMV+PCSEARGHTGY
Sbjct: 241 RTYEVREDKMECSQVDGGGSVGLPPCKRRLRSNQANDMSEDPSTTVMVKPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTF+RLRCIS
Sbjct: 301 LTFSRLRCIS 310

[Close]
BLAST of Spg008194 vs. ExPASy TrEMBL
Match: A0A6J1H9F4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.6e-164
Identity = 290/310 (93.55%), Postives = 302/310 (97.42%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCE+STFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 391 LTFARLRCIS 401
           LTFARLRC+S
Sbjct: 301 LTFARLRCLS 309

[Close]
BLAST of Spg008194 vs. ExPASy TrEMBL
Match: A0A6J1J9F9 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484759 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.4e-164
Identity = 290/309 (93.85%), Postives = 301/309 (97.41%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPTD+ KKISFTRSIRDGDLVIVYERHD+MKAVKVCESSTFQNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCESSTFQNRFGMFKHSDWIGKPFG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SLTTSLARAVAPTGHV+TFDFHEQRAASAREDFEKTGLTSLVTV VRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGGSVGIPPCKRRLRSNEANDMNDDSSTTVMVRPCSEARGHTGY 390
           RTYEVREGK ESSQV+GGGSV +PPCK+R+RS EAND N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 391 LTFARLRCI 400
           LTFARLRC+
Sbjct: 301 LTFARLRCL 308

[Close]
BLAST of Spg008194 vs. TAIR 10
Match: AT5G14600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.9e-136
Identity = 236/318 (74.21%), Postives = 271/318 (85.22%), Query Frame = 0 

Query: 91  MLPTDESKKISFTRSIRDGDLVIVYERHDIMKAVKVCESSTFQNRFGVFKHSDWIGKPFG 150
           MLPT+  K  SF R I DGDLVIVYERHD+MK VKV +    QNRFGV+KHSDWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 151 SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 210
           +KV SNKG FVYLLAPTPELWTLVLSHRTQILYIADISFV+M+LE+VPGC+VLESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 211 SLTTSLARAVAPTGHVHTFDFHEQRAASAREDFEKTGLTSLVTVRVRDIQGQGFPEEFVG 270
           SL+TSLARAVAPTGHV++FDFHEQRA SAREDFEKTG++SLVTV VRDIQG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 271 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 330
           LADSVFLDLPQPWLA+PSA +MLKQDGVLCSFSPCIEQVQR+ E LRS F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 331 RTYEVREGKPESSQVDGGG----SVGIPPCKRRLRSNE----ANDMNDDSSTTVMVRPCS 390
           RTYEV+E K ++S + G      + G+ PCKR+ RSNE    + D + ++S+ VM RPCS
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCS 300

Query: 391 EARGHTGYLTFARLRCIS 401
           EARGHTGYLTFARL+C++
Sbjct: 301 EARGHTGYLTFARLQCLA 318

The following BLAST results are available for this feature:
BLAST of Spg008194 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038894928.14.9e-16593.55tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa h... [more]
XP_022987109.12.4e-16493.87tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A-like isoform... [more]
KAE8653334.14.1e-16492.60hypothetical protein Csa_023207, partial [Cucumis sativus][more]
KAG7023747.15.4e-16493.55tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, partial [Cu... [more]
XP_022158112.15.4e-16493.23tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Momordica c... [more]
BLAST of Spg008194 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q80XC23.5e-6546.98tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu... [more]
Q96FX77.8e-6546.64tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi... [more]
Q6AY462.5e-6345.51tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no... [more]
A6H7911.2e-6045.30tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru... [more]
Q86JJ01.2e-6042.16tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste... [more]
BLAST of Spg008194 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LYQ81.1e-16591.02(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3... [more]
A0A6J1JHY41.2e-16493.87tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1DWC12.6e-16493.23tRNA (adenine(58)-N(1))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1H9F42.6e-16493.55tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J9F93.4e-16493.85tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
BLAST of Spg008194 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G14600.13.9e-13674.21S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PFAMPF08704GCD14coord: 159..395
e-value: 4.5E-97
score: 324.5
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PANTHERPTHR12133TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASEcoord: 91..397
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PROSITEPS51620SAM_TRM61coord: 109..397
score: 99.860229
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 168..398
e-value: 6.7E-81
score: 273.0
NoneNo IPR availableGENE3D3.10.330.20coord: 100..167
e-value: 1.2E-25
score: 91.3
NoneNo IPR availablePIRSRPIRSR017269-1PIRSR017269-1coord: 137..338
e-value: 3.6E-40
score: 135.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 339..374
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..36
NoneNo IPR availablePANTHERPTHR12133:SF2TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61Acoord: 91..397
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 202..299
e-value: 0.0079908
score: 33.9427
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 105..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008194.1Spg008194.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0016429 tRNA (adenine-N1-)-methyltransferase activity