Spg008155 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008155
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Proteinase inhibitor I25, cystatin
Location	scaffold1: 53417123 .. 53417428 (+)
RNA-Seq Expression	Spg008155
Synteny	Spg008155

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTTTGAGCCGATTGAGGATATCAACGATCCACGCGTGCAAGGAATTGGAAGCTTTGCAGTGGAGAGAAGTGGAGAAAATTTGAAGTTCAAACGCGTGGTAAATGGTCTGAAACAGCCTACCTTCGGTGTTTTAATTTGTAAATGGTTTATATATCGTCTTGTGGTGGAGGCTGAAAAAGCTAATGGAATTAATTGGACATATGAGACTGAGGTGTATGTGTGTATCAACCCATTTGACGGAAACTGGGAACCAGAACGTAGCCAACTCAAATATTTTAAGTCAATCCTCGAGTATGAGTAG

mRNA sequence

ATGAGTTTTGAGCCGATTGAGGATATCAACGATCCACGCGTGCAAGGAATTGGAAGCTTTGCAGTGGAGAGAAGTGGAGAAAATTTGAAGTTCAAACGCGTGGTAAATGGTCTGAAACAGCCTACCTTCGGTGTTTTAATTTGTAAATGGTTTATATATCGTCTTGTGGTGGAGGCTGAAAAAGCTAATGGAATTAATTGGACATATGAGACTGAGGTGTATGTGTGTATCAACCCATTTGACGGAAACTGGGAACCAGAACGTAGCCAACTCAAATATTTTAAGTCAATCCTCGAGTATGAGTAG

Coding sequence (CDS)

ATGAGTTTTGAGCCGATTGAGGATATCAACGATCCACGCGTGCAAGGAATTGGAAGCTTTGCAGTGGAGAGAAGTGGAGAAAATTTGAAGTTCAAACGCGTGGTAAATGGTCTGAAACAGCCTACCTTCGGTGTTTTAATTTGTAAATGGTTTATATATCGTCTTGTGGTGGAGGCTGAAAAAGCTAATGGAATTAATTGGACATATGAGACTGAGGTGTATGTGTGTATCAACCCATTTGACGGAAACTGGGAACCAGAACGTAGCCAACTCAAATATTTTAAGTCAATCCTCGAGTATGAGTAG

Protein sequence

MSFEPIEDINDPRVQGIGSFAVERSGENLKFKRVVNGLKQPTFGVLICKWFIYRLVVEAEKANGINWTYETEVYVCINPFDGNWEPERSQLKYFKSILEYE

Homology

[Close]

BLAST of Spg008155 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 69.3 bits (168), Expect = 2.1e-08
Identity = 40/82 (48.78%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 3  FEPIEDINDPRVQGIGSFAVE----RSGENLKFKRVVNGL-------KQPTFGVLICKWF 62
          FEPI+DINDP+VQ IG+  V+    ++GENLKF RVVNGL         P  G       
Sbjct: 7  FEPIKDINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNGLFAGFVDPFSPLGGT------ 66

Query: 63 IYRLVVEAEKANGINWTYETEV 74
          +Y LV+EA  A  INWTY+ +V
Sbjct: 67 VYELVLEAINAGKINWTYKVKV 82

[Close]

BLAST of Spg008155 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 69.3 bits (168), Expect = 2.1e-08
Identity = 38/76 (50.00%), Postives = 47/76 (61.84%), Query Frame = 0


Query: 4  EPIEDINDPRVQGIGSFAVE-----RSGENLKFKRVVNGLKQPTF-GVLICKWFIYRLVV 63
          +PI DINDP VQ +G  AVE      SGE LKF RVVNGLK   + G    +  +Y LV+
Sbjct: 11 KPIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVL 70

Query: 64 EAEKANGINWTYETEV 74
          EA+    INWTY T++
Sbjct: 71 EAKTNEEINWTYATKL 86

[Close]

BLAST of Spg008155 vs. NCBI nr
Match: KAA0040521.1 (Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-07
Identity = 32/74 (43.24%), Postives = 48/74 (64.86%), Query Frame = 0


Query: 5  PIEDINDPRVQGIGSFAV----ERSGENLKFKRVVNGLKQPTFGVLICKWFIYRLVVEAE 64
          PI+D++   +QG+G FAV    E  GENL+F +V+NG +Q   GV I    I+ +V+ A+
Sbjct: 13 PIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFEQGRRGVRI----IFTIVLSAK 72

Query: 65 KANGINWTYETEVY 75
            NGI WTY+ +V+
Sbjct: 73 NCNGIIWTYKAKVF 82

[Close]

BLAST of Spg008155 vs. NCBI nr
Match: XP_038897049.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 64.3 bits (155), Expect = 6.8e-07
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 3   FEPIEDINDPRVQGIGSFAV----ERSGENLKFKRVVNGLKQPTFGVLICKWFIYRLVVE 62
           +EPIE+I DP VQ +G F+V    +++G NLKF RVVNG KQ   G+       YRL +E
Sbjct: 27  WEPIENIEDPYVQEMGRFSVMEHNKQTGANLKFIRVVNGEKQVVAGI------NYRLRIE 86

Query: 63  AEKANGINWTYETEVYVCINPFDGNWEPERSQLKYFKSILE 100
           A     + WTYE  V       D  WE ++ +L YF  +L+
Sbjct: 87  ARNEMNLVWTYEAMVN------DRPWE-KKWKLIYFVPLLK 114

[Close]

BLAST of Spg008155 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 63.9 bits (154), Expect = 8.9e-07
Identity = 47/115 (40.87%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 2   SFEPIEDINDP-RVQGIGSFAVE----RSGENLKFKRVVNGLK-------QPTFGVLICK 61
           +F+PIEDIND   VQ +G+  V+    ++GENLKF RVVNGL         P  GVL   
Sbjct: 6   AFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVL--- 65

Query: 62  WFIYRLVVEAEKANGINWTYETEV---YVCINPFDGNWEPERSQLKYFKSILEYE 102
              Y LV+EA   + INWTY+  V        PF   +       + FK +LEYE
Sbjct: 66  ---YELVLEAITTSKINWTYKARVSKSKAITPPFHMLY-----SFQSFKPVLEYE 109

[Close]

BLAST of Spg008155 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.0e-06
Identity = 35/97 (36.08%), Postives = 49/97 (50.52%), Query Frame = 0


Query: 3   FEPIEDINDPRVQGIGSFAVER----SGENLKFKRVVNGLKQPTFGVLICKWFIYRLVVE 62
           + PI D+ DP +Q +G +AVER    S + L+F+RV +G +Q   G+       YRLVV 
Sbjct: 35  WSPITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVVSGM------NYRLVVS 94

Query: 63  AEKANGINWTYETEVYVCINPFDGNWEPERSQLKYFK 96
           A    G   +Y   VY      + +W   R QL  FK
Sbjct: 95  ASDPAGATASYVAVVY------EQSWTNTR-QLTSFK 118

[Close]

BLAST of Spg008155 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-04
Identity = 31/78 (39.74%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 2  SFEPIEDINDPRVQGIGSFAVER-----SGENLKFKRVVNGLKQPTFGVLICKWFIYRLV 61
          S+EP+ DINDP VQ +G +AV       +   L F+RV +G KQ   GV       YRL 
Sbjct: 29 SWEPV-DINDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGV------NYRLT 88

Query: 62 VEAEKANGINWTYETEVY 75
          +EA  +   +  YE  VY
Sbjct: 89 LEASSSGAKDGRYEAVVY 99

[Close]

BLAST of Spg008155 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-08
Identity = 38/76 (50.00%), Postives = 47/76 (61.84%), Query Frame = 0


Query: 4  EPIEDINDPRVQGIGSFAVE-----RSGENLKFKRVVNGLKQPTF-GVLICKWFIYRLVV 63
          +PI DINDP VQ +G  AVE      SGE LKF RVVNGLK   + G    +  +Y LV+
Sbjct: 11 KPIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVL 70

Query: 64 EAEKANGINWTYETEV 74
          EA+    INWTY T++
Sbjct: 71 EAKTNEEINWTYATKL 86

[Close]

BLAST of Spg008155 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.7e-08
Identity = 38/76 (50.00%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 4  EPIEDINDPRVQGIGSFAVE-----RSGENLKFKRVVNGLKQPTF-GVLICKWFIYRLVV 63
          +PI DINDP VQ IG  AVE       GE LKF RVVNGLK   + G    +  +Y LV+
Sbjct: 11 KPIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVL 70

Query: 64 EAEKANGINWTYETEV 74
          EA+    INWTY T++
Sbjct: 71 EAKTNEEINWTYATKL 86

[Close]

BLAST of Spg008155 vs. ExPASy TrEMBL
Match: A0A5A7TFU6 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 8.7e-08
Identity = 32/74 (43.24%), Postives = 48/74 (64.86%), Query Frame = 0


Query: 5  PIEDINDPRVQGIGSFAV----ERSGENLKFKRVVNGLKQPTFGVLICKWFIYRLVVEAE 64
          PI+D++   +QG+G FAV    E  GENL+F +V+NG +Q   GV I    I+ +V+ A+
Sbjct: 13 PIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFEQGRRGVRI----IFTIVLSAK 72

Query: 65 KANGINWTYETEVY 75
            NGI WTY+ +V+
Sbjct: 73 NCNGIIWTYKAKVF 82

[Close]

BLAST of Spg008155 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.3e-07
Identity = 47/115 (40.87%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 2   SFEPIEDINDP-RVQGIGSFAVE----RSGENLKFKRVVNGLK-------QPTFGVLICK 61
           +F+PIEDIND   VQ +G+  V+    ++GENLKF RVVNGL         P  GVL   
Sbjct: 6   AFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVL--- 65

Query: 62  WFIYRLVVEAEKANGINWTYETEV---YVCINPFDGNWEPERSQLKYFKSILEYE 102
              Y LV+EA   + INWTY+  V        PF   +       + FK +LEYE
Sbjct: 66  ---YELVLEAITTSKINWTYKARVSKSKAITPPFHMLY-----SFQSFKPVLEYE 109

[Close]

BLAST of Spg008155 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.3e-07
Identity = 47/115 (40.87%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 2   SFEPIEDINDP-RVQGIGSFAVE----RSGENLKFKRVVNGLK-------QPTFGVLICK 61
           +F+PIEDIND   VQ +G+  V+    ++GENLKF RVVNGL         P  GVL   
Sbjct: 6   AFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVL--- 65

Query: 62  WFIYRLVVEAEKANGINWTYETEV---YVCINPFDGNWEPERSQLKYFKSILEYE 102
              Y LV+EA   + INWTY+  V        PF   +       + FK +LEYE
Sbjct: 66  ---YELVLEAITTSKINWTYKARVSKSKAITPPFHMLY-----SFQSFKPVLEYE 109

[Close]

BLAST of Spg008155 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.2e-04
Identity = 27/71 (38.03%), Postives = 37/71 (52.11%), Query Frame = 0


Query: 3  FEPIEDINDPRVQGIGSFAV----ERSGENLKFKRVVNGLKQPTFGVLICKWFIYRLVVE 62
          + PI ++ DP+V  IG FAV    +RS   LKF+ VV+G  Q   G        YRL V 
Sbjct: 31 WSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGT------NYRLKVA 90

Query: 63 AEKANGINWTY 70
          A   +G++  Y
Sbjct: 91 ANDGDGVSKNY 95

The following BLAST results are available for this feature:

BLAST of Spg008155 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649716.1	2.1e-08	48.78	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
KGN54823.1	2.1e-08	50.00	hypothetical protein Csa_012607 [Cucumis sativus]	[more]
KAA0040521.1	1.8e-07	43.24	Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. maku...	[more]
XP_038897049.1	6.8e-07	41.58	cysteine proteinase inhibitor 5-like [Benincasa hispida]	[more]
XP_008442277.1	8.9e-07	40.87	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]

BLAST of Spg008155 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10J94	6.0e-06	36.08	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
Q10Q47	1.1e-04	39.74	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of Spg008155 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ98	1.0e-08	50.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]
A0A0A0L191	6.7e-08	50.00	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]
A0A5A7TFU6	8.7e-08	43.24	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 S...	[more]
A0A5A7TJK0	4.3e-07	40.87	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5B9	4.3e-07	40.87	cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=...	[more]

BLAST of Spg008155 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	1.2e-04	38.03	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 1..97 e-value: 4.9E-10 score: 41.5
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 5..75
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 6..74 e-value: 8.5E-9 score: 35.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008155.1	Spg008155.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity