Spg008070 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg008070 vs. NCBI nr
Match: KAG6589160.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 514/538 (95.54%), Postives = 525/538 (97.58%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR IGF WSFTFLFL +SLSCINGEDPYRF+TWN+TYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MRGIGFFWSFTFLFLVLSLSCINGEDPYRFYTWNVTYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPG+NFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGRNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPF TPAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFATPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTS LHYSN+A+
Sbjct: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSTLHYSNAAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQ DWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQTDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNS SFIPADTPLKLADY+KISGVFSLGSIPDYPTGSGA+LQTSVIAADFRG
Sbjct: 361 INGKQRYAVNSASFIPADTPLKLADYYKISGVFSLGSIPDYPTGSGAYLQTSVIAADFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPED  QSWHVDGHNFFVVGMDGG+WTPASRLTYNLRDTISRCTVQVYPKSWT
Sbjct: 421 YAEVVFENPEDTEQSWHVDGHNFFVVGMDGGEWTPASRLTYNLRDTISRCTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENW RQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRAVGL
Sbjct: 481 AVYMPLDNVGMWNIRSENWVRQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAVGL 538

BLAST of Spg008070 vs. NCBI nr
Match: XP_023531945.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 512/538 (95.17%), Postives = 523/538 (97.21%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR IGF WSFTFLFL +SLSCINGEDPYRF+TWN+TYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MRGIGFFWSFTFLFLVLSLSCINGEDPYRFYTWNVTYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPG+NFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGRNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAG FGGFKIQSRSIIPVPF TPAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGAFGGFKIQSRSIIPVPFATPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGR  NGNTFTVEQGKTYRFRISNVGL TAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLITAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTS LHYSN+A+
Sbjct: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSTLHYSNAAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQ DWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQTDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNS SFIPADTPLKLADY+KISGVFSLGSIPDYPTGSGA+LQTSVIAADFRG
Sbjct: 361 INGKQRYAVNSASFIPADTPLKLADYYKISGVFSLGSIPDYPTGSGAYLQTSVIAADFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPED  QSWHVDGHNFFVVGMDGG+WTPASRLTYNLRDTISRCTVQVYPKSWT
Sbjct: 421 YAEVVFENPEDTEQSWHVDGHNFFVVGMDGGEWTPASRLTYNLRDTISRCTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENW RQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRAVGL
Sbjct: 481 AVYMPLDNVGMWNIRSENWVRQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAVGL 538

BLAST of Spg008070 vs. NCBI nr
Match: XP_022930723.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 512/538 (95.17%), Postives = 523/538 (97.21%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR IG  WSFTFLFL +SLSCINGEDPYRF+TWN+TYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MRGIGLFWSFTFLFLVLSLSCINGEDPYRFYTWNVTYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPG+NFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGRNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAG FGGFKIQSRSIIPVPF TPAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGAFGGFKIQSRSIIPVPFATPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTS LHYSN+A+
Sbjct: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSTLHYSNAAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQ DWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQTDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNS SFIPADTPLKLADY+KISGVFSLGSIPDYPTGSGA+LQTSVIAADFRG
Sbjct: 361 INGKQRYAVNSASFIPADTPLKLADYYKISGVFSLGSIPDYPTGSGAYLQTSVIAADFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPED  QSWHVDGHNFFVVGMDGG+WTPASRLTYNLRDTISRCTVQVYPKSWT
Sbjct: 421 YAEVVFENPEDTEQSWHVDGHNFFVVGMDGGEWTPASRLTYNLRDTISRCTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENW RQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRAVGL
Sbjct: 481 AVYMPLDNVGMWNIRSENWVRQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAVGL 538

BLAST of Spg008070 vs. NCBI nr
Match: XP_022988735.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 511/538 (94.98%), Postives = 523/538 (97.21%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR IGF WSFTFLFL +SLSCINGEDPYRF+TWN+TYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MRGIGFFWSFTFLFLVLSLSCINGEDPYRFYTWNVTYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPG+NFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGKNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAG FGGFKI SRSIIPVPF TPAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGAFGGFKIHSRSIIPVPFTTPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRGPNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTS LHYSN+A+
Sbjct: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSTLHYSNAAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQ DWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQTDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNS SFIPADTPLKLADY+KISGVFSLGSIPDYPTGSGA+LQTSV+AADFRG
Sbjct: 361 INGKQRYAVNSASFIPADTPLKLADYYKISGVFSLGSIPDYPTGSGAYLQTSVMAADFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPED  QSWHVDGHNFFVVGMDGG+WTPASRLTYNLRDTISRCTVQVYPKSWT
Sbjct: 421 YAEVVFENPEDTEQSWHVDGHNFFVVGMDGGEWTPASRLTYNLRDTISRCTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENW RQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRAVGL
Sbjct: 481 AVYMPLDNVGMWNIRSENWVRQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAVGL 538

BLAST of Spg008070 vs. NCBI nr
Match: XP_008455444.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 510/534 (95.51%), Postives = 523/534 (97.94%), Query Frame = 0

Query: 6   FLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVT 65
           FL+S T LFL +S SCI GEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIE+VT
Sbjct: 6   FLYSLTSLFLVLSFSCIYGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIEAVT 65

Query: 66  NENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSY 125
           NENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSY
Sbjct: 66  NENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSY 125

Query: 126 FYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGS 185
           FYFPSLA HKAAGGFGGFKIQSRSIIP+PFPTPAGEFTVLAGDW+SKNHTDLRAILDGGS
Sbjct: 126 FYFPSLALHKAAGGFGGFKIQSRSIIPIPFPTPAGEFTVLAGDWYSKNHTDLRAILDGGS 185

Query: 186 DLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHT 245
           DLP PDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHT
Sbjct: 186 DLPSPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHT 245

Query: 246 LQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSVSGP 305
           LQNTYES+D+HLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSA+SVSGP
Sbjct: 246 LQNTYESIDVHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAASVSGP 305

Query: 306 PPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQ 365
           PPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQ
Sbjct: 306 PPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQ 365

Query: 366 RYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRGYAEVV 425
           RYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGA+LQTSV+AADFRGYAEVV
Sbjct: 366 RYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAYLQTSVMAADFRGYAEVV 425

Query: 426 FENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAVYMP 485
           FENPED VQSWHVDGHNFFVVGMDGGQWTPA+RLTYNLRDTISRCTVQVYPKSWTAVYMP
Sbjct: 426 FENPEDVVQSWHVDGHNFFVVGMDGGQWTPANRLTYNLRDTISRCTVQVYPKSWTAVYMP 485

Query: 486 LDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLN 540
           LDNVGMWNIRSENWARQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRA+GLN
Sbjct: 486 LDNVGMWNIRSENWARQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAIGLN 539

BLAST of Spg008070 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 9.6e-175
Identity = 293/542 (54.06%), Postives = 377/542 (69.56%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR +  L +  +L  A ++  ++ EDPY    WN+TYG   PLGV QQ ILINGQFPGP 
Sbjct: 1   MRGVKLLAACLYLAAAATV-VVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPN 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           I S +N N+IINVFN+LDEPFL++WNGIQ R+N WQDG  GT CPI PG N+TY  Q KD
Sbjct: 61  INSTSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFY+P+   H+AAGG+GG ++ SR +IPVP+  P  ++TVL GDW++K+HT L+  
Sbjct: 121 QIGSYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKF 180

Query: 181 LDGGSDLPFPDGLLINGRASNGN-----TFTVEQGKTYRFRISNVGLTTAINFRIQGHKM 240
           LDGG  +  PDG++ING++  G+      FT++ GKTYR RI NVG+ T+INFRIQ HKM
Sbjct: 181 LDGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKM 240

Query: 241 LLVEVEGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHY 300
            LVE+EG+H LQN Y+SLD+H+GQ +  +VTA+Q P+DYY+V S+RF   ++TTT +L Y
Sbjct: 241 KLVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRY 300

Query: 301 SNSASSVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQ 360
                  S   P GP +   WSL Q RS R NLTA+  RPNPQGSYHYG IN+T TI+L 
Sbjct: 301 EGGKGPASSQLPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLV 360

Query: 361 STAPIINGKQRYAVNSVSFIPADTPLKLADYFKISG-VFSLGSIPDYPTG---SGAHLQT 420
           +T   ++GK R+A+N VS    +TPLKLA+YF IS  VF   +I D PT        ++ 
Sbjct: 361 NTQGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEP 420

Query: 421 SVIAADFRGYAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCT 480
           +V+    R + EVVFEN E +VQSWH+DG++FF V ++ G WTP  R  YNL D +SR T
Sbjct: 421 NVLNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHT 480

Query: 481 VQVYPKSWTAVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALR 534
           VQVYPK W A+ +  DN GMWN+RSEN  R+YLGQQLY  V SP  S RDEY +P  +L+
Sbjct: 481 VQVYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQ 540

BLAST of Spg008070 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.4e-170
Identity = 282/542 (52.03%), Postives = 373/542 (68.82%), Query Frame = 0

Query: 9   SFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTNEN 68
           +F  L L +S+  I  EDPY +F WN+TYG I PLGV QQGILINGQFPGP+I   +N N
Sbjct: 7   TFVALLLCLSVGVI-AEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNN 66

Query: 69  LIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYFYF 128
           +++NVFN+LDEPFL +WNG+Q R+NSWQDG  GT CPI PGQNFTY  QVKDQIGSY YF
Sbjct: 67  IVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYF 126

Query: 129 PSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSDLP 188
           P+ A H+AAGG+G   + SR++IPVPF  PA E+ V  GDW++K H  L+ ILDGG  + 
Sbjct: 127 PTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIG 186

Query: 189 FPDGLLINGRASNGN-----TFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGT 248
            PDG++ING+++         FT+E GKTYR+R  N+G+ +++N R QGH M LVE+EG+
Sbjct: 187 RPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGS 246

Query: 249 HTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNS---AS 308
           HT+QN Y+SLD+H+GQ  SVLVTADQ P+DYY+VVS+RF  Q L++ +I+ Y+N    AS
Sbjct: 247 HTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPAS 306

Query: 309 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 368
                PP   T  I WS+ Q RS R NLTA+  RPNPQGSYHYG IN+T TI++ ++   
Sbjct: 307 PELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQ 366

Query: 369 INGKQRYAVNSVSFIPADTPLKLADYFKISG-VFSLGSIPDYPTGSGAHL--QTSVIAAD 428
           + GK RY +N +S    +TPLKL +YF  +   F    + D      + L   T+V  A 
Sbjct: 367 VGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNAT 426

Query: 429 FRGYAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPK 488
           +R + E++FEN E  ++++H+DG++FF V ++ G+W+P  R  YNL D +SR  +QVYP 
Sbjct: 427 YRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPN 486

Query: 489 SWTAVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVG 540
           SW A+ +  DN GMWN+RSE W + YLG+QLY  V SP+ S RDEY IP N   CG   G
Sbjct: 487 SWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 546

BLAST of Spg008070 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-156
Identity = 278/547 (50.82%), Postives = 360/547 (65.81%), Query Frame = 0

Query: 7   LWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTN 66
           L+    L   V++S     DPY F+ + ++Y    PLGV QQ I ING+FPGP I   TN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 67  ENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYF 126
           ENL++NV N LDE  L+ WNGIQQRR SWQDGV GTNCPIPP  N+TY  QVKDQIGS+F
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 127 YFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSD 186
           YFPSL F +A+GGFG F +  R+IIPVPF TP G+ TV  GDW+ +NHT LR  LD G D
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 187 LPFPDGLLINGRAS------------NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHK 246
           L  PDG+LING+              +  T TV  GKTYR R+SNVG++T++NFRIQGH 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 247 MLLVEVEGTHTLQNTYESLDIHLGQSYSVLVTADQ-PPQDYYIVVSTRFTSQIL----TT 306
           ++L E EG++T+Q  Y SLDIH+GQSYS LVT DQ    DYYIV S R  ++ +    T 
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 307 TSILHYSNSASSVSGPPPGGPTIQID--WSLEQARSLRRNLTANGPRPNPQGSYHYGLIN 366
             IL Y+NS     G  P GP  + D  +S+ QARS+R N++A+G RPNPQGS+ YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 367 VTHTIRLQSTAPI-INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSG 426
           VT    L++  P+ I+GK+R  +N +SF    TP++LAD  K+  V+ L   P  P    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPKRPLTGP 422

Query: 427 AHLQTSVIAADFRGYAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDT 486
           A + TS+I   +RG+ EVV +N +  +QS+H+ G+ FFVVGMD G+WT  SR TYN  D 
Sbjct: 423 AKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDG 482

Query: 487 ISRCTVQVYPKSWTAVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIP 534
           I+R T+QVYP +W+A+ + LDN G WN+R+EN    YLGQ+ Y+RV +P  + + E+  P
Sbjct: 483 IARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHP 542

BLAST of Spg008070 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.5e-154
Identity = 274/543 (50.46%), Postives = 360/543 (66.30%), Query Frame = 0

Query: 11  TFLFLAVSLSCIN-GEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTNENL 70
           +FL     LS ++   DP+  + + ++Y    PLGV QQ I +NGQFPGP + + TN N+
Sbjct: 10  SFLLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNV 69

Query: 71  IINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYFYFP 130
           ++NVFN LDEP L++W GIQ RRNSWQDGV GTNCPIPP  NFTY  QVKDQIGS+FY P
Sbjct: 70  VVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSP 129

Query: 131 SLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSDLPF 190
           SL F +A+GGFG   I +R IIP+PFP P GE   + GDW++++H  LR  LD G +L  
Sbjct: 130 SLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGM 189

Query: 191 PDGLLINGRAS---NGN--------TFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLV 250
           PDG+LING+     N +        TF VE GKTYR R+ NVG++T++NFRIQ H +LLV
Sbjct: 190 PDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLV 249

Query: 251 EVEGTHTLQNTYESLDIHLGQSYSVLVTADQ-PPQDYYIVVSTRFTS----QILTTTSIL 310
           E EG +T Q  +   D+H+GQSYS LVT DQ    DYYIV S RF +    Q +T  +IL
Sbjct: 250 ETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAIL 309

Query: 311 HYSNSASSVSGPPPGGPT-IQIDWS-LEQARSLRRNLTANGPRPNPQGSYHYGLINVTHT 370
           HYSNS   VSGP P   T +   WS + Q +++R+N +A+G RPNPQGS+HYG IN+T+T
Sbjct: 310 HYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNT 369

Query: 371 IRLQSTAP-IINGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQ 430
             L+S  P IING  R  +N +SF+   TP++LAD  K+ G + L   PD P      L 
Sbjct: 370 YILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRPLRLD 429

Query: 431 TSVIAADFRGYAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRC 490
            S+I A ++G+ +VVF+N +  +QS+HVDG++FFVVGMD G W+   + +YN  D ISR 
Sbjct: 430 RSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRS 489

Query: 491 TVQVYPKSWTAVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNAL 534
           T++VYP  WTAV + LDNVG+WNIR EN  R YLG++ Y+R+ +P    + E   P N L
Sbjct: 490 TIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVL 549

BLAST of Spg008070 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.7e-151
Identity = 267/553 (48.28%), Postives = 356/553 (64.38%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           M A  F ++F F F  +      G DPY  + + ++Y    PLGV QQ I +NG+FPGP 
Sbjct: 1   MAATDFFFAFVFSFALIFGFSFAG-DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPV 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           I + TN N+ +NV N LDEP L++W G+Q RRNSWQDGV GTNCPIPP  NFTY  Q+KD
Sbjct: 61  INATTNYNVHVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFY PSL F +A+GGFG   I +R ++P+PF  P GE   + GDW+++NHT LR I
Sbjct: 121 QIGSYFYSPSLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRI 180

Query: 181 LDGGSDLPFPDGLLINGRAS-----------NGNTFTVEQGKTYRFRISNVGLTTAINFR 240
           LD G +L  PDG+LING+                T  V+ GKTYR R+ NVG++T++NFR
Sbjct: 181 LDSGKELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFR 240

Query: 241 IQGHKMLLVEVEGTHTLQNTYESLDIHLGQSYSVLVTADQ-PPQDYYIVVSTRFTS---- 300
           IQ HK+LL+E EG +T Q  +   D+H+GQSYS LVT DQ    DYYIV S RF +    
Sbjct: 241 IQNHKLLLIETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVW 300

Query: 301 QILTTTSILHYSNSASSVSGPPPGGPT-IQIDWS-LEQARSLRRNLTANGPRPNPQGSYH 360
           Q +T   ILHYSNS    SGP P   T +   WS + Q R++++N +A+G RPNPQGS+H
Sbjct: 301 QRVTGVGILHYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFH 360

Query: 361 YGLINVTHTIRLQSTAPI-INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDY 420
           YG IN+T T  L+S  P  INGK R  +N +SF+   TP++LAD  K+ G + L   PD 
Sbjct: 361 YGQINITRTYILRSLPPTKINGKLRATLNGISFVNPSTPMRLADDHKVKGDYML-DFPDR 420

Query: 421 PTGSG-AHLQTSVIAADFRGYAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLT 480
           P       L +S+I A ++G+ +V+F+N +  +QS+H+DG+ F+VV MD G W+     +
Sbjct: 421 PLDEKLPRLSSSIINATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSS 480

Query: 481 YNLRDTISRCTVQVYPKSWTAVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWR 534
           YN  D ++R TV+VYP +WTAV + LDNVG+WNIR EN  R YLGQ+ Y+R+ +P  +  
Sbjct: 481 YNNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGS 540

BLAST of Spg008070 vs. ExPASy TrEMBL
Match: A0A6J1EW63 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111437116 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 512/538 (95.17%), Postives = 523/538 (97.21%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR IG  WSFTFLFL +SLSCINGEDPYRF+TWN+TYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MRGIGLFWSFTFLFLVLSLSCINGEDPYRFYTWNVTYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPG+NFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGRNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAG FGGFKIQSRSIIPVPF TPAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGAFGGFKIQSRSIIPVPFATPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTS LHYSN+A+
Sbjct: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSTLHYSNAAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQ DWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQTDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNS SFIPADTPLKLADY+KISGVFSLGSIPDYPTGSGA+LQTSVIAADFRG
Sbjct: 361 INGKQRYAVNSASFIPADTPLKLADYYKISGVFSLGSIPDYPTGSGAYLQTSVIAADFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPED  QSWHVDGHNFFVVGMDGG+WTPASRLTYNLRDTISRCTVQVYPKSWT
Sbjct: 421 YAEVVFENPEDTEQSWHVDGHNFFVVGMDGGEWTPASRLTYNLRDTISRCTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENW RQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRAVGL
Sbjct: 481 AVYMPLDNVGMWNIRSENWVRQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAVGL 538

BLAST of Spg008070 vs. ExPASy TrEMBL
Match: A0A6J1JKE6 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111485985 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 511/538 (94.98%), Postives = 523/538 (97.21%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           MR IGF WSFTFLFL +SLSCINGEDPYRF+TWN+TYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MRGIGFFWSFTFLFLVLSLSCINGEDPYRFYTWNVTYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPG+NFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGKNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAG FGGFKI SRSIIPVPF TPAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGAFGGFKIHSRSIIPVPFTTPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRGPNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTS LHYSN+A+
Sbjct: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSTLHYSNAAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQ DWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQTDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNS SFIPADTPLKLADY+KISGVFSLGSIPDYPTGSGA+LQTSV+AADFRG
Sbjct: 361 INGKQRYAVNSASFIPADTPLKLADYYKISGVFSLGSIPDYPTGSGAYLQTSVMAADFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPED  QSWHVDGHNFFVVGMDGG+WTPASRLTYNLRDTISRCTVQVYPKSWT
Sbjct: 421 YAEVVFENPEDTEQSWHVDGHNFFVVGMDGGEWTPASRLTYNLRDTISRCTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENW RQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRAVGL
Sbjct: 481 AVYMPLDNVGMWNIRSENWVRQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAVGL 538

BLAST of Spg008070 vs. ExPASy TrEMBL
Match: A0A1S3C0H2 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103495602 PE=3 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 510/534 (95.51%), Postives = 523/534 (97.94%), Query Frame = 0

Query: 6   FLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVT 65
           FL+S T LFL +S SCI GEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIE+VT
Sbjct: 6   FLYSLTSLFLVLSFSCIYGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIEAVT 65

Query: 66  NENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSY 125
           NENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSY
Sbjct: 66  NENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSY 125

Query: 126 FYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGS 185
           FYFPSLA HKAAGGFGGFKIQSRSIIP+PFPTPAGEFTVLAGDW+SKNHTDLRAILDGGS
Sbjct: 126 FYFPSLALHKAAGGFGGFKIQSRSIIPIPFPTPAGEFTVLAGDWYSKNHTDLRAILDGGS 185

Query: 186 DLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHT 245
           DLP PDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHT
Sbjct: 186 DLPSPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHT 245

Query: 246 LQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSVSGP 305
           LQNTYES+D+HLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSA+SVSGP
Sbjct: 246 LQNTYESIDVHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAASVSGP 305

Query: 306 PPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQ 365
           PPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQ
Sbjct: 306 PPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQ 365

Query: 366 RYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRGYAEVV 425
           RYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGA+LQTSV+AADFRGYAEVV
Sbjct: 366 RYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAYLQTSVMAADFRGYAEVV 425

Query: 426 FENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAVYMP 485
           FENPED VQSWHVDGHNFFVVGMDGGQWTPA+RLTYNLRDTISRCTVQVYPKSWTAVYMP
Sbjct: 426 FENPEDVVQSWHVDGHNFFVVGMDGGQWTPANRLTYNLRDTISRCTVQVYPKSWTAVYMP 485

Query: 486 LDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLN 540
           LDNVGMWNIRSENWARQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRA+GLN
Sbjct: 486 LDNVGMWNIRSENWARQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAIGLN 539

BLAST of Spg008070 vs. ExPASy TrEMBL
Match: A0A6J1C3B7 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111007875 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.1e-310
Identity = 510/538 (94.80%), Postives = 522/538 (97.03%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           M  + FL   TFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ
Sbjct: 1   MGEVRFLCLLTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD
Sbjct: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGSYFYFPSLAFHKAAG FGGFKI SRSIIPVPFP PAGEFTVLAGDW+SKNHTDLRAI
Sbjct: 121 QIGSYFYFPSLAFHKAAGAFGGFKILSRSIIPVPFPPPAGEFTVLAGDWYSKNHTDLRAI 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV
Sbjct: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQN Y+SLDIHLGQSYSVLVTADQP QDYYIVVSTRFTSQ+LTTTSILHYSNSA+
Sbjct: 241 EGTHTLQNIYDSLDIHLGQSYSVLVTADQPQQDYYIVVSTRFTSQVLTTTSILHYSNSAA 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
           SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYG+INVTHTIRLQSTAPI
Sbjct: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGIINVTHTIRLQSTAPI 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSI DYPTG G +LQTSV+AA+FRG
Sbjct: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSISDYPTGGGGYLQTSVMAANFRG 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           YAEVVFENPEDA+QSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYP+SWT
Sbjct: 421 YAEVVFENPEDAMQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPQSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGL 539
           AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSP NSWRDEYPIP NALRCGRA+GL
Sbjct: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPVNSWRDEYPIPRNALRCGRAIGL 538

BLAST of Spg008070 vs. ExPASy TrEMBL
Match: A0A5D3C5D0 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G004310 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.3e-303
Identity = 500/537 (93.11%), Postives = 516/537 (96.09%), Query Frame = 0

Query: 6   FLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVT 65
           FL+S T LFL +S SCI GEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIE+VT
Sbjct: 6   FLYSLTSLFLVLSFSCIYGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIEAVT 65

Query: 66  NENLIINVFNSLD---EPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQI 125
           NENLIINVFN  +      L+  NGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQI
Sbjct: 66  NENLIINVFNKYELFSTLILLCRNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQI 125

Query: 126 GSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILD 185
           GSYFYFPSLA HKAAGGFGGFKIQSRSIIP+PFPTPAGEFTVLAGDW+SKNHTDLRAILD
Sbjct: 126 GSYFYFPSLALHKAAGGFGGFKIQSRSIIPIPFPTPAGEFTVLAGDWYSKNHTDLRAILD 185

Query: 186 GGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEG 245
           GGSDLP PDGLLINGR  NGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEG
Sbjct: 186 GGSDLPSPDGLLINGRGLNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEG 245

Query: 246 THTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSV 305
           THTLQNTYES+D+HLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSA+SV
Sbjct: 246 THTLQNTYESIDVHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAASV 305

Query: 306 SGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIIN 365
           SGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIIN
Sbjct: 306 SGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIIN 365

Query: 366 GKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRGYA 425
           GKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGA+LQTSV+AADFRGYA
Sbjct: 366 GKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAYLQTSVMAADFRGYA 425

Query: 426 EVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAV 485
           EVVFENPED VQSWHVDGHNFFVVGM+GGQWTPA+RLTYNLRDTISRCTVQVYPKSWTAV
Sbjct: 426 EVVFENPEDVVQSWHVDGHNFFVVGMNGGQWTPANRLTYNLRDTISRCTVQVYPKSWTAV 485

Query: 486 YMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLN 540
           YMPLDNVGMWNIRSENWARQYLGQQLYLRVYSP NSWRDEYPIPSNAL+CGRA+GLN
Sbjct: 486 YMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPVNSWRDEYPIPSNALKCGRAIGLN 542

BLAST of Spg008070 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 838.2 bits (2164), Expect = 3.8e-243
Identity = 391/532 (73.50%), Postives = 450/532 (84.59%), Query Frame = 0

Query: 13  LFLAVS-LSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTNENLII 72
           LF+ +S L  +  EDPYRFF WNITYGDI+PLGV+QQGILING FPGP I SVTN+NLII
Sbjct: 11  LFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDNLII 70

Query: 73  NVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYFYFPSL 132
           NV+NSLDEPFL+SWNGIQQRRNS+ DGVYGT CPIPPG+N+TY+LQ+KDQIGS++YFPSL
Sbjct: 71  NVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSL 130

Query: 133 AFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSDLPFPD 192
            FHKAAGGFGG +I SR  IPVPFP PAG+ TVL GDW+  NHTDLRA LD G  LP PD
Sbjct: 131 GFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPD 190

Query: 193 GLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHTLQNTYE 252
           G+LINGR S+G T  VEQGKTYRFRISNVGL  ++NFRIQ HKM +VEVEGTHTLQ T+ 
Sbjct: 191 GILINGR-SSGATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFS 250

Query: 253 SLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSVSGPPPGGPT 312
           SLD+H+GQSYSVLVTADQ P+DYY+VVS+RFTS +LTTT I  YSNSA  VSGP PGGPT
Sbjct: 251 SLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGGPT 310

Query: 313 IQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQRYAVNS 372
           IQIDWSL QAR++R NL+A+GPRPNPQGSYHYG+IN T TIRL S+A  ++GKQRYAVNS
Sbjct: 311 IQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNS 370

Query: 373 VSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRGYAEVVFENPED 432
           VSF PADTPLK+ADYFKI GV+  GSI   PTG G +L TSV+  D+R + E++FEN ED
Sbjct: 371 VSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSED 430

Query: 433 AVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAVYMPLDNVGM 492
            VQSWH+DG++F+VVGMDGGQW+P SR  YNLRD ++RCTVQVYP SWTA+ + LDNVGM
Sbjct: 431 IVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGM 490

Query: 493 WNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLNTRPL 544
           WN+RSE WARQYLGQQLYLRVY+P+ S RDEYPIP NAL CGRA G +TRPL
Sbjct: 491 WNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGRSTRPL 541

BLAST of Spg008070 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 815.8 bits (2106), Expect = 2.0e-236
Identity = 380/533 (71.29%), Postives = 449/533 (84.24%), Query Frame = 0

Query: 11  TFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTNENLI 70
           + + L V ++ + G++PYRFFTW ITYGDI+PLGVKQQGILINGQFPGP I+++TN+N+I
Sbjct: 8   SIVLLLVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNII 67

Query: 71  INVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYFYFPS 130
           I+VFN L EPFLISWNG+QQR+NSWQDGV GT CPIPPG+NFTYV+QVKDQIGS++YFPS
Sbjct: 68  ISVFNYLKEPFLISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPS 127

Query: 131 LAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSDLPFP 190
           LAFHKAAG FG  ++ SR  IPVPF  P G+F +LAGDW+  NH  LR +L+ G +LP P
Sbjct: 128 LAFHKAAGAFGAIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNLPNP 187

Query: 191 DGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHTLQNTY 250
           DG+LINGR   GNTFTV+ GKTYRFRISNVG+ T++NFRIQGH M LVEVEG+HT+QN Y
Sbjct: 188 DGVLINGRGWGGNTFTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQNIY 247

Query: 251 ESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSVSGPPPGGP 310
            SLDIHLGQSYSVLVTA+Q PQDYYIV+S+RFT ++LTTTSILHYSNS   VSGP P GP
Sbjct: 248 TSLDIHLGQSYSVLVTANQAPQDYYIVISSRFTRKVLTTTSILHYSNSRKGVSGPVPNGP 307

Query: 311 TIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQRYAVN 370
           T+ I  SL QAR++RRNLTA+GPRPNPQGSYHYGLI    TI L ++AP INGKQRYAVN
Sbjct: 308 TLDIASSLYQARTIRRNLTASGPRPNPQGSYHYGLIKPGRTIILANSAPWINGKQRYAVN 367

Query: 371 SVSFIPADTPLKLADYFKISGVFSLGSIPDYPT-GSGAHLQTSVIAADFRGYAEVVFENP 430
             SF+  DTPLKLADYFKI GVF+LGSIP  P+ G+G +LQ+SV+AA+FR + EVVF+N 
Sbjct: 368 GASFVAPDTPLKLADYFKIPGVFNLGSIPTSPSGGNGGYLQSSVMAANFREFIEVVFQNW 427

Query: 431 EDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAVYMPLDNV 490
           E++VQSWHV G++FFVVGMDGGQWTP SR  YNLRD +SR TVQVYP++WTA+Y+ LDNV
Sbjct: 428 ENSVQSWHVSGYSFFVVGMDGGQWTPGSRAKYNLRDAVSRSTVQVYPRAWTAIYIALDNV 487

Query: 491 GMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLNTRP 543
           GMWNIRSENWARQYLGQQ YLRVY+ + S+RDEYP P NAL CGRA G +TRP
Sbjct: 488 GMWNIRSENWARQYLGQQFYLRVYTSSTSYRDEYPPPKNALMCGRAKGRHTRP 540

BLAST of Spg008070 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 800.0 bits (2065), Expect = 1.1e-231
Identity = 375/543 (69.06%), Postives = 441/543 (81.22%), Query Frame = 0

Query: 1   MRAIGFLWSFTFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQ 60
           M A+G       LF     + +  E PYRFF WN+TYGDI+PLGV+QQGILINGQFPGP 
Sbjct: 1   MMAVGRSGGTILLFCLSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPD 60

Query: 61  IESVTNENLIINVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKD 120
           I SVTN+NLIINV NSLDEPFLISWNG+Q RRNS+ DG+YGT CPIPP  N+TY+LQVKD
Sbjct: 61  IHSVTNDNLIINVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKD 120

Query: 121 QIGSYFYFPSLAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAI 180
           QIGS++YFPSLAFHKAAGGFGG +I SR  IPVPF  PAG++TVL GDW+  NHTDL++ 
Sbjct: 121 QIGSFYYFPSLAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSR 180

Query: 181 LDGGSDLPFPDGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEV 240
           LD G  LP PDG+LINGR SNG T  VEQGKTYR RISNVGL  ++NFRIQ H+M LVEV
Sbjct: 181 LDRGRKLPSPDGILINGR-SNGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEV 240

Query: 241 EGTHTLQNTYESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSAS 300
           EGTHTLQ  + SLD+H+GQSYSVL+TADQ P+DYY+VVS+RFT +I+TTT +L YS S++
Sbjct: 241 EGTHTLQTMFSSLDVHVGQSYSVLITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSST 300

Query: 301 SVSGPPPGGPTIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPI 360
             SGP PGGPTIQ+DWSL QAR++R NLTA+GPRPNPQGSYHYGLI +  TI   S+A  
Sbjct: 301 PASGPIPGGPTIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQ 360

Query: 361 INGKQRYAVNSVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRG 420
           INGKQRY VNSVSF+PADTPLKLAD+FKISGV+ + SI D PT  G +L TSV+  D+R 
Sbjct: 361 INGKQRYGVNSVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRT 420

Query: 421 YAEVVFENPEDAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWT 480
           + E+VFEN ED VQS+H++G++F+VVGMDGGQW   SR  YNLRD +SR TVQVYPKSWT
Sbjct: 421 FIEIVFENQEDIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWT 480

Query: 481 AVYMPLDNVGMWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLNT 540
           A+Y+ LDNVGMWN+RSE WARQYLGQQLYLRV++ + S RDEYPIP N+  CGRA G +T
Sbjct: 481 AIYIALDNVGMWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRARGRHT 540

Query: 541 RPL 544
           RPL
Sbjct: 541 RPL 542

BLAST of Spg008070 vs. TAIR 10
Match: AT1G21850.1 (SKU5 similar 8 )

HSP 1 Score: 772.3 bits (1993), Expect = 2.6e-223
Identity = 358/522 (68.58%), Postives = 425/522 (81.42%), Query Frame = 0

Query: 19  LSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTNENLIINVFNSLD 78
           +S +  EDPY+FF W++TYG+I PL V QQGILING+FPGP I +VTN+NLIINVFN LD
Sbjct: 19  ISFVAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTNDNLIINVFNHLD 78

Query: 79  EPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYFYFPSLAFHKAAG 138
           EPFLISW+GI+  RNS+QDGVYGT CPIPPG+N+TY LQVKDQIGS++YFPSL FHKAAG
Sbjct: 79  EPFLISWSGIRNWRNSYQDGVYGTTCPIPPGKNYTYALQVKDQIGSFYYFPSLGFHKAAG 138

Query: 139 GFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSDLPFPDGLLINGR 198
           GFG  +I SR  IPVPFP PAG++TVL GDW+  NH DLRA LD G  LPFPDG+LINGR
Sbjct: 139 GFGAIRISSRPRIPVPFPAPAGDYTVLIGDWYKTNHKDLRAQLDNGGKLPFPDGILINGR 198

Query: 199 ASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHTLQNTYESLDIHLG 258
            S G T  +E GKTYR RISNVGL  ++NFRIQ HKM LVEVEGTHT+Q  + SLD+H+G
Sbjct: 199 GS-GATLNIEPGKTYRLRISNVGLQNSLNFRIQNHKMKLVEVEGTHTIQTPFSSLDVHVG 258

Query: 259 QSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSVSGPPPGGPTIQIDWSL 318
           QSYSVL+TADQP +DYYIVVS+RFTS+IL T  +LHYSNSA  VSGP P  P IQ+ WS 
Sbjct: 259 QSYSVLITADQPAKDYYIVVSSRFTSKILITAGVLHYSNSAGPVSGPIPEAP-IQLRWSF 318

Query: 319 EQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQRYAVNSVSFIPAD 378
           +QAR+++ NL A+GPRPNPQG+YHYG I VT TI+L S+A  INGKQRYAVNS SF P D
Sbjct: 319 DQARAIKTNLAASGPRPNPQGTYHYGKIKVTRTIKLASSAGNINGKQRYAVNSASFYPTD 378

Query: 379 TPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRGYAEVVFENPEDAVQSWHV 438
           TPLKLADYFKI+GV++ GSIPD PT    +  TSV+  D++ + E+VFEN ED VQ+WH+
Sbjct: 379 TPLKLADYFKIAGVYNPGSIPDQPTHGAIYPVTSVMQTDYKAFVEIVFENWEDIVQTWHL 438

Query: 439 DGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAVYMPLDNVGMWNIRSEN 498
           DG++FFVVGM+ G+W+ ASR  YNL D +SRCTVQVYP+SWTA+Y+ LDNVGMWN+RSE 
Sbjct: 439 DGYSFFVVGMELGKWSAASRKVYNLNDAVSRCTVQVYPRSWTAIYVSLDNVGMWNLRSEL 498

Query: 499 WARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRAVGLNT 541
           W RQYLGQQ Y+RVY+P+ S RDEY IP NAL CGRA G +T
Sbjct: 499 WERQYLGQQFYMRVYTPSTSLRDEYLIPKNALLCGRATGHHT 538

BLAST of Spg008070 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 768.1 bits (1982), Expect = 4.8e-222
Identity = 358/525 (68.19%), Postives = 425/525 (80.95%), Query Frame = 0

Query: 11  TFLFLAVSLSCINGEDPYRFFTWNITYGDIWPLGVKQQGILINGQFPGPQIESVTNENLI 70
           T LFL   +S    EDPYRFF W++TYG+I PLGV QQGILING+FPGP I S+TN+NLI
Sbjct: 14  TLLFL---ISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITNDNLI 73

Query: 71  INVFNSLDEPFLISWNGIQQRRNSWQDGVYGTNCPIPPGQNFTYVLQVKDQIGSYFYFPS 130
           INVFN LDEPFL+SWNGI+  +NS+QDGVYGT CPIPPG+N+TY LQVKDQIGS++YFPS
Sbjct: 74  INVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYYFPS 133

Query: 131 LAFHKAAGGFGGFKIQSRSIIPVPFPTPAGEFTVLAGDWFSKNHTDLRAILDGGSDLPFP 190
           L FHKAAGGFGG +I SR++IPVPFPTPA ++T+L GDW+  NH DL+A LD G  LP P
Sbjct: 134 LGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPLP 193

Query: 191 DGLLINGRASNGNTFTVEQGKTYRFRISNVGLTTAINFRIQGHKMLLVEVEGTHTLQNTY 250
           DG+LINGR S+G T  +E GKTYR RISNVGL  ++NFRIQ H M LVEVEG +T+QN +
Sbjct: 194 DGILINGR-SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNLF 253

Query: 251 ESLDIHLGQSYSVLVTADQPPQDYYIVVSTRFTSQILTTTSILHYSNSASSVSGPPPGGP 310
            SLD+H+GQSYSVL+TADQP +DYY+VVS+RFTS+ILTTT +LHYSNS + VSGP P GP
Sbjct: 254 SSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDGP 313

Query: 311 TIQIDWSLEQARSLRRNLTANGPRPNPQGSYHYGLINVTHTIRLQSTAPIINGKQRYAVN 370
            I++ WS  QAR++R NLTA+GPRPNPQGSY YG+IN+T TIRL +    I GKQRYAVN
Sbjct: 314 -IKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGKQRYAVN 373

Query: 371 SVSFIPADTPLKLADYFKISGVFSLGSIPDYPTGSGAHLQTSVIAADFRGYAEVVFENPE 430
           S SF PADTPLKL DYFKI GV+  GSI D PT       TSV+ ADFR + EV+FEN E
Sbjct: 374 SASFYPADTPLKLVDYFKIDGVYKPGSISDQPTNGAIFPTTSVMQADFRAFVEVIFENSE 433

Query: 431 DAVQSWHVDGHNFFVVGMDGGQWTPASRLTYNLRDTISRCTVQVYPKSWTAVYMPLDNVG 490
           D VQSWH+DG++F+VVGM+ G+W+PASR  YNL D I RCT+QVYP+SWTA+Y+ LDNVG
Sbjct: 434 DIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNVG 493

Query: 491 MWNIRSENWARQYLGQQLYLRVYSPANSWRDEYPIPSNALRCGRA 536
           MWN+RSE W RQYLGQQ Y+RVY+ + S RDEY IP NAL CGRA
Sbjct: 494 MWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCGRA 533