Spg008010 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008010
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	BHLH domain-containing protein
Location	scaffold4: 7856435 .. 7857344 (+)
RNA-Seq Expression	Spg008010
Synteny	Spg008010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCCCATTTCCAGTTCTCGGCTTCGCCGTCGTCGGCCGCAAAGGTCGAAAGGCGAGTCGTCGAGAAAAACAGAAGAAATCAGATGAAAAACCTCTGTTCTCAGCTCAATTCCCTCCTCCCAAATCAGGATTCCAAGGTCCTTTCTTATTTCCAAAACTTTCCATTTTTTATCTCATTTGCTCATTGACAGAGCATCCTCTGTTCTTGTGCTCTGTTTTTCTTGAAGAAACTTTGATGAATTATGCCACGAATTTTACCAAAATCTTGATGTTTCTTTGTGCAGGAGATTTCATTGACATTGCCGGATCAGATCGATGAAGCAATTAAGTACATAAAAGAGCTGGAGAAGAAAGTGAATTCAGCTAAGGAGAAGAAGGAAAAACTACAGGGGAAAAACAAGAGCTTCTTGAATATGGATTCTTCGTCTTCGTCGTCGTCTTCGTCTGCGTCGCCTCAGCTCAAAATCCATCAAATGGGTCGAAGTTTGGAGATAATTTTAAGCAGTGGATTGGATAATCAGTATCTCTTCTGTGAAACCATTCGCATTCTTCAAGAAGAAGGCACTGAAGTTGTCAATGCCAGCTTCTCTGTTTCTGGGAACTCTGTTTTTCACACCCTCCATGCACAAGTAAATCTCAATTCTCCCTTAAACTTACACGTCAAAAAAATGACATTACACGAGTTCGAACACAGGACCTGGTACTATTTTTATTCGTATTCCTGACGAGATTTGTGGTTTGAACGTACAGCTCGGAGACTCGGAGGTCGAGTTCGAAGCGGCGAAAGTGACCGAGAGGCTGAAGCGATTGGTTTACGGATCGAACAGCGACATGGAATCGCAGAAGGAGAAGCAGTGGTGGGATGAATTCCCATCCGAATTCCCATCTGATAATTGGGATTTTTAA

mRNA sequence

ATGGATCCCCATTTCCAGTTCTCGGCTTCGCCGTCGTCGGCCGCAAAGGTCGAAAGGCGAGTCGTCGAGAAAAACAGAAGAAATCAGATGAAAAACCTCTGTTCTCAGCTCAATTCCCTCCTCCCAAATCAGGATTCCAAGGAGATTTCATTGACATTGCCGGATCAGATCGATGAAGCAATTAAGTACATAAAAGAGCTGGAGAAGAAAGTGAATTCAGCTAAGGAGAAGAAGGAAAAACTACAGGGGAAAAACAAGAGCTTCTTGAATATGGATTCTTCGTCTTCGTCGTCGTCTTCGTCTGCGTCGCCTCAGCTCAAAATCCATCAAATGGGTCGAAGTTTGGAGATAATTTTAAGCAGTGGATTGGATAATCAGTATCTCTTCTGTGAAACCATTCGCATTCTTCAAGAAGAAGGCACTGAAGTTGTCAATGCCAGCTTCTCTGTTTCTGGGAACTCTGTTTTTCACACCCTCCATGCACAACTCGGAGACTCGGAGGTCGAGTTCGAAGCGGCGAAAGTGACCGAGAGGCTGAAGCGATTGGTTTACGGATCGAACAGCGACATGGAATCGCAGAAGGAGAAGCAGTGGTGGGATGAATTCCCATCCGAATTCCCATCTGATAATTGGGATTTTTAA

Coding sequence (CDS)

ATGGATCCCCATTTCCAGTTCTCGGCTTCGCCGTCGTCGGCCGCAAAGGTCGAAAGGCGAGTCGTCGAGAAAAACAGAAGAAATCAGATGAAAAACCTCTGTTCTCAGCTCAATTCCCTCCTCCCAAATCAGGATTCCAAGGAGATTTCATTGACATTGCCGGATCAGATCGATGAAGCAATTAAGTACATAAAAGAGCTGGAGAAGAAAGTGAATTCAGCTAAGGAGAAGAAGGAAAAACTACAGGGGAAAAACAAGAGCTTCTTGAATATGGATTCTTCGTCTTCGTCGTCGTCTTCGTCTGCGTCGCCTCAGCTCAAAATCCATCAAATGGGTCGAAGTTTGGAGATAATTTTAAGCAGTGGATTGGATAATCAGTATCTCTTCTGTGAAACCATTCGCATTCTTCAAGAAGAAGGCACTGAAGTTGTCAATGCCAGCTTCTCTGTTTCTGGGAACTCTGTTTTTCACACCCTCCATGCACAACTCGGAGACTCGGAGGTCGAGTTCGAAGCGGCGAAAGTGACCGAGAGGCTGAAGCGATTGGTTTACGGATCGAACAGCGACATGGAATCGCAGAAGGAGAAGCAGTGGTGGGATGAATTCCCATCCGAATTCCCATCTGATAATTGGGATTTTTAA

Protein sequence

MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILSSGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRLVYGSNSDMESQKEKQWWDEFPSEFPSDNWDF

Homology

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BLAST of Spg008010 vs. NCBI nr
Match: XP_038888628.1 (transcription factor bHLH162-like [Benincasa hispida])

HSP 1 Score: 336.3 bits (861), Expect = 2.0e-88
Identity = 178/212 (83.96%), Postives = 193/212 (91.04%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MDP+FQFSAS SSAAK+ERR++EKNRRNQMKNLCSQLNSLLPN DSK+ISL LPDQIDEA
Sbjct: 1   MDPNFQFSASSSSAAKIERRIIEKNRRNQMKNLCSQLNSLLPNHDSKDISLPLPDQIDEA 60

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           IKYIKELEKKV+SAKEKK +LQGKNKS +NMD  SSSSSSSASPQL IHQMG+S+EI+LS
Sbjct: 61  IKYIKELEKKVSSAKEKKNQLQGKNKSSINMD--SSSSSSSASPQLNIHQMGKSVEIVLS 120

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLFCET+RILQEEGTEV+NASFSVSGNSVFHT+HAQLGDS VEF   K TERLK
Sbjct: 121 SGLDNQYLFCETLRILQEEGTEVINASFSVSGNSVFHTIHAQLGDSMVEFGVTKATERLK 180

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSEFPSDNWD 213
           RLVYGSNSD+E QKEKQWWDEFP E    NWD
Sbjct: 181 RLVYGSNSDVEVQKEKQWWDEFPCE----NWD 206

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BLAST of Spg008010 vs. NCBI nr
Match: XP_022930718.1 (transcription factor bHLH162-like [Cucurbita moschata])

HSP 1 Score: 332.0 bits (850), Expect = 3.7e-87
Identity = 178/205 (86.83%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MD HFQFS S SSAAK+ERR+VEKNRRNQMK LCSQLNSLLPNQDSKEISL LPDQIDEA
Sbjct: 50  MDRHFQFSTSSSSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 109

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           +KYIKELEKKVN AKEKKEKL+GKNKS +N++SSSSSSSSSASPQLKIHQ GRSLEIILS
Sbjct: 110 VKYIKELEKKVNLAKEKKEKLEGKNKSSMNIESSSSSSSSSASPQLKIHQSGRSLEIILS 169

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLF ETI ILQEEG EVVNASFSVSG+SVF T HAQL  S +EFEAAKVTERLK
Sbjct: 170 SGLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLK 229

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSE 206
           RLVYGSNSDME QKEKQWWDEFPS+
Sbjct: 230 RLVYGSNSDMELQKEKQWWDEFPSD 254

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BLAST of Spg008010 vs. NCBI nr
Match: KAG6589225.1 (Transcription factor basic helix-loop-helix 162, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022925.1 Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323.9 bits (829), Expect = 1.0e-84
Identity = 176/204 (86.27%), Postives = 186/204 (91.18%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MD HFQFS   +SAAK+ERR+VEKNRRNQMK LCSQLNSLLPNQDSKEISL LPDQIDEA
Sbjct: 1   MDRHFQFS---TSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 60

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           +KYIKELEKKVN AKEKKEKL+GKNKS +N++SSSSSSSSSASPQLKIHQ GRSLEIILS
Sbjct: 61  VKYIKELEKKVNLAKEKKEKLEGKNKSSMNIESSSSSSSSSASPQLKIHQSGRSLEIILS 120

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLF ETI ILQEEG EVVNASFSVSG+SVF T HAQL  S +EFEAAKVTERLK
Sbjct: 121 SGLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLK 180

Query: 181 RLVYGSNSDMESQKEKQWWDEFPS 205
           RLVYGSNSDME QKEKQWWDEFPS
Sbjct: 181 RLVYGSNSDMELQKEKQWWDEFPS 201

[Close]

BLAST of Spg008010 vs. NCBI nr
Match: XP_023531689.1 (transcription factor bHLH162-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323.2 bits (827), Expect = 1.7e-84
Identity = 175/205 (85.37%), Postives = 185/205 (90.24%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MD HFQFS S SSAAK+ERR+VEKNRRNQMK LCSQLNSLLPNQDSKEISL LPDQIDEA
Sbjct: 46  MDRHFQFSTSSSSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 105

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           +KYIKELEKKVN AKEKKEKL+GKNKS +N++   SSSSSSASPQLKIHQ GRSLEIILS
Sbjct: 106 VKYIKELEKKVNLAKEKKEKLEGKNKSSMNIE---SSSSSSASPQLKIHQSGRSLEIILS 165

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLF ETI ILQEEG EVVNASFSVSG+SVF T HAQL  S +EFEAAKVTERLK
Sbjct: 166 SGLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLK 225

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSE 206
           RLVYGSNSDME QKEKQWWDEFPS+
Sbjct: 226 RLVYGSNSDMELQKEKQWWDEFPSD 247

[Close]

BLAST of Spg008010 vs. NCBI nr
Match: XP_022988952.1 (transcription factor bHLH162-like [Cucurbita maxima])

HSP 1 Score: 318.5 bits (815), Expect = 4.2e-83
Identity = 172/205 (83.90%), Postives = 185/205 (90.24%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MD HFQFS S SSAAK+ERR++EKNRRNQMK LCSQLNSLLPNQDSKEISL+LPDQIDEA
Sbjct: 1   MDRHFQFSTSSSSAAKIERRIIEKNRRNQMKTLCSQLNSLLPNQDSKEISLSLPDQIDEA 60

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           +KYIKELEKKVN AKEKKEKL+GKNKS +N++   SSSS SASPQLKIHQ GRSLEIILS
Sbjct: 61  VKYIKELEKKVNLAKEKKEKLEGKNKSPMNIE---SSSSCSASPQLKIHQSGRSLEIILS 120

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLF ETI ILQ+EG EVVNASFSVSG+SVF T HAQL  S +EFEAAKVTERLK
Sbjct: 121 SGLDNQYLFSETISILQKEGVEVVNASFSVSGSSVFQTFHAQLEGSIIEFEAAKVTERLK 180

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSE 206
           RLVYGSNSDME QKEKQWWDEFPS+
Sbjct: 181 RLVYGSNSDMELQKEKQWWDEFPSD 202

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BLAST of Spg008010 vs. ExPASy Swiss-Prot
Match: F4JIJ7 (Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.1e-23
Identity = 72/183 (39.34%), Postives = 115/183 (62.84%), Query Frame = 0


Query: 9   ASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELE 68
           ++   +  V+R+ VEKNRR QMK+L S+L SLLP+  S E  LTLPDQ+DEA  YIK+L+
Sbjct: 6   SNTGQSRSVDRKTVEKNRRMQMKSLYSELISLLPHHSSTE-PLTLPDQLDEAANYIKKLQ 65

Query: 69  KKVNSAKEKKEKLQGKN--KSFLNMDSSSSSSSSSAS-----PQLKIHQMGRSLEIILSS 128
             V   +E+K  L      +   ++ SSS SSS   S     P+++I + G    I L +
Sbjct: 66  VNVEKKRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVT 125

Query: 129 GLDNQYLFCETIRILQEE-GTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 184
            L+++++FCE IR+L EE G E+ +A +S+  ++VFHTLH ++ + +     +++ ERL+
Sbjct: 126 SLEHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEHDYG-ARSQIPERLE 185

[Close]

BLAST of Spg008010 vs. ExPASy Swiss-Prot
Match: F4I4E1 (Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 50/175 (28.57%), Postives = 95/175 (54.29%), Query Frame = 0


Query: 12  SSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELEKKV 71
           SS+ + +R + EK+RR +MK+L S L+S +    S    L +P  ID+A  Y+ +L++ V
Sbjct: 12  SSSLREQRNLREKDRRMRMKHLFSILSSHV----SPTRKLPVPHLIDQATSYMIQLKENV 71

Query: 72  NSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILSSGLD-NQYLFC 131
           N  KEKK       ++ L  +  +    S   P+L I     ++E+ L   L+  + +  
Sbjct: 72  NYLKEKK-------RTLLQGELGNLYEGSFLLPKLSIRSRDSTIEMNLIMDLNMKRVMLH 131

Query: 132 ETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRLVYG 186
           E + I +EEG +V++A+     +   +T+ AQ   S +  + +++ ER+++++YG
Sbjct: 132 ELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRIEERVRKIIYG 175

[Close]

BLAST of Spg008010 vs. ExPASy Swiss-Prot
Match: A2WZ60 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=39946 GN=IRO2 PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-07
Identity = 49/182 (26.92%), Postives = 89/182 (48.90%), Query Frame = 0


Query: 8   SASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKEL 67
           S    S  K+     E++RR Q+  L S L +LLP+ D  +  L++P  +   +KYI EL
Sbjct: 62  SGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTK-KLSIPTTVSRVLKYIPEL 121

Query: 68  EKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLK----IHQMGRSLEIILSSGL 127
           +K+V + + KK++L   + +        S   S     +     I+ M   +++ L S +
Sbjct: 122 QKQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEIMVQVSLLSNV 181

Query: 128 DNQYL-FCETIRILQEEGTEVVNASFSVS-GNSVFHTLHAQLGDSEVEFEAAKVTERLKR 184
               L   + I++L+ EG   +++S S   GN  F+++H Q  +  +  E     ERL++
Sbjct: 182 AGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFCERLEK 241

[Close]

BLAST of Spg008010 vs. ExPASy Swiss-Prot
Match: Q0JFZ0 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=39947 GN=IRO2 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-07
Identity = 49/182 (26.92%), Postives = 89/182 (48.90%), Query Frame = 0


Query: 8   SASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKEL 67
           S    S  K+     E++RR Q+  L S L +LLP+ D  ++S  +P  +   +KYI EL
Sbjct: 62  SGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTKLS--IPTTVSRVLKYIPEL 121

Query: 68  EKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLK----IHQMGRSLEIILSSGL 127
           +K+V + + KK++L   + +        S   S     +     I+ M   +++ L S +
Sbjct: 122 QKQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEIMVQVSLLSNV 181

Query: 128 DNQYL-FCETIRILQEEGTEVVNASFSVS-GNSVFHTLHAQLGDSEVEFEAAKVTERLKR 184
               L   + I++L+ EG   +++S S   GN  F+++H Q  +  +  E     ERL++
Sbjct: 182 AGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFCERLEK 241

[Close]

BLAST of Spg008010 vs. ExPASy Swiss-Prot
Match: Q9XIJ1 (Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.3e-07
Identity = 47/173 (27.17%), Postives = 93/173 (53.76%), Query Frame = 0


Query: 12  SSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELEKKV 71
           +S+ + +R + EK RR +MK+L S L+S +    S    L +P  ID+A+ Y+ +L++KV
Sbjct: 12  ASSLREQRNLREKERRMRMKHLFSILSSHV----SPTRRLPVPQLIDQAVSYMIQLKEKV 71

Query: 72  NSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILSSGLDNQ-YLFC 131
           N   E K ++ G        +  + S  SS  P+L I  +   +E+ L   L+ +  +  
Sbjct: 72  NYLNEMKRRMLGG-------EVKNRSEGSSLLPKLSIRSLDSIIEMNLVMDLNMKGVMLH 131

Query: 132 ETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRLV 184
           + + + +EEG +V++A+     +  F+T+ AQ     +  + +++ ERL+ ++
Sbjct: 132 KLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDPSRIEERLRDII 173

[Close]

BLAST of Spg008010 vs. ExPASy TrEMBL
Match: A0A6J1EW58 (transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111437113 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.8e-87
Identity = 178/205 (86.83%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MD HFQFS S SSAAK+ERR+VEKNRRNQMK LCSQLNSLLPNQDSKEISL LPDQIDEA
Sbjct: 50  MDRHFQFSTSSSSAAKIERRIVEKNRRNQMKTLCSQLNSLLPNQDSKEISLALPDQIDEA 109

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           +KYIKELEKKVN AKEKKEKL+GKNKS +N++SSSSSSSSSASPQLKIHQ GRSLEIILS
Sbjct: 110 VKYIKELEKKVNLAKEKKEKLEGKNKSSMNIESSSSSSSSSASPQLKIHQSGRSLEIILS 169

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLF ETI ILQEEG EVVNASFSVSG+SVF T HAQL  S +EFEAAKVTERLK
Sbjct: 170 SGLDNQYLFSETISILQEEGVEVVNASFSVSGSSVFQTFHAQLEGSMIEFEAAKVTERLK 229

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSE 206
           RLVYGSNSDME QKEKQWWDEFPS+
Sbjct: 230 RLVYGSNSDMELQKEKQWWDEFPSD 254

[Close]

BLAST of Spg008010 vs. ExPASy TrEMBL
Match: A0A6J1JEF7 (transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111486157 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 2.1e-83
Identity = 172/205 (83.90%), Postives = 185/205 (90.24%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MD HFQFS S SSAAK+ERR++EKNRRNQMK LCSQLNSLLPNQDSKEISL+LPDQIDEA
Sbjct: 1   MDRHFQFSTSSSSAAKIERRIIEKNRRNQMKTLCSQLNSLLPNQDSKEISLSLPDQIDEA 60

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           +KYIKELEKKVN AKEKKEKL+GKNKS +N++   SSSS SASPQLKIHQ GRSLEIILS
Sbjct: 61  VKYIKELEKKVNLAKEKKEKLEGKNKSPMNIE---SSSSCSASPQLKIHQSGRSLEIILS 120

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SGLDNQYLF ETI ILQ+EG EVVNASFSVSG+SVF T HAQL  S +EFEAAKVTERLK
Sbjct: 121 SGLDNQYLFSETISILQKEGVEVVNASFSVSGSSVFQTFHAQLEGSIIEFEAAKVTERLK 180

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSE 206
           RLVYGSNSDME QKEKQWWDEFPS+
Sbjct: 181 RLVYGSNSDMELQKEKQWWDEFPSD 202

[Close]

BLAST of Spg008010 vs. ExPASy TrEMBL
Match: A0A5D3C788 (Transcription factor bHLH55-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003340 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.7e-83
Identity = 168/212 (79.25%), Postives = 186/212 (87.74%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MDP+FQFSAS S+AAK+ERR++EKNRRNQMKNLC QL SL+P QDSKE+SL LPDQID A
Sbjct: 1   MDPNFQFSASSSTAAKIERRIIEKNRRNQMKNLCDQLKSLVPQQDSKEVSLALPDQIDVA 60

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           IKYIK+LEKKVNSAKEKK +LQGKNKS +NMDSSSSS     SPQLKI+QMG+SLEI+LS
Sbjct: 61  IKYIKDLEKKVNSAKEKKNRLQGKNKSAINMDSSSSS-----SPQLKINQMGKSLEIVLS 120

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SG+DNQYL CET+RILQEEG EVVNASFSVSG SVFHT+HA+LGDS VEF   K TERLK
Sbjct: 121 SGIDNQYLLCETLRILQEEGIEVVNASFSVSGKSVFHTIHAELGDSMVEFGTTKATERLK 180

Query: 181 RLVYGSNSDMESQKEKQWWDEFPSEFPSDNWD 213
           RLVYGSNSD+E QKEKQWWDEFPSE    NWD
Sbjct: 181 RLVYGSNSDVELQKEKQWWDEFPSE----NWD 203

[Close]

BLAST of Spg008010 vs. ExPASy TrEMBL
Match: A0A0A0K2C9 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G048070 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 6.0e-83
Identity = 170/214 (79.44%), Postives = 190/214 (88.79%), Query Frame = 0


Query: 1   MDPHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEA 60
           MDP+FQFSAS S+AAK+ERR++EKNRRNQMKNLC QL SL+P QDSKE+SL LPDQID A
Sbjct: 1   MDPNFQFSASSSTAAKIERRIIEKNRRNQMKNLCDQLKSLVPQQDSKEVSLALPDQIDVA 60

Query: 61  IKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILS 120
           IKYIK+LEK+VNSAKEKK +LQGKNKS +NMDSSSSSSSSS+SPQLKI+QMG+SLEIILS
Sbjct: 61  IKYIKDLEKRVNSAKEKKNRLQGKNKSAINMDSSSSSSSSSSSPQLKINQMGKSLEIILS 120

Query: 121 SGLDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 180
           SG DNQYL CET+RIL+EEGTEVV+ASFSVSGNSVFHT+HAQLGDS VEF   K TERL 
Sbjct: 121 SGNDNQYLLCETLRILEEEGTEVVSASFSVSGNSVFHTIHAQLGDSMVEFGMTKATERLT 180

Query: 181 RLVYGSNSDMESQK-EKQWWDEFPSEFPSDNWDF 214
           RLVY SNSD+E QK EKQWW EFPSE    NW+F
Sbjct: 181 RLVYRSNSDVELQKEEKQWWKEFPSE----NWEF 210

[Close]

BLAST of Spg008010 vs. ExPASy TrEMBL
Match: A0A1S3C1C4 (transcription factor bHLH55-like OS=Cucumis melo OX=3656 GN=LOC103495505 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.1e-80
Identity = 164/210 (78.10%), Postives = 183/210 (87.14%), Query Frame = 0


Query: 3   PHFQFSASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIK 62
           P+FQFSAS S+AAK+ERR++EKNRRNQMKNLC QL SL+P Q SKE+SL LPDQID AIK
Sbjct: 4   PNFQFSASSSTAAKIERRIIEKNRRNQMKNLCDQLKSLVPQQYSKEVSLALPDQIDVAIK 63

Query: 63  YIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILSSG 122
           YIK+LEKKVNSAKEKK +LQGKNKS +NMDSSSSS     SPQLKI+QMG+SLEI+LSSG
Sbjct: 64  YIKDLEKKVNSAKEKKNRLQGKNKSAINMDSSSSS-----SPQLKINQMGKSLEIVLSSG 123

Query: 123 LDNQYLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRL 182
           +DNQYL CET+RILQEEG EVVNASFSVSG SVFHT+HA+LGDS VEF   K TERLKRL
Sbjct: 124 IDNQYLLCETLRILQEEGIEVVNASFSVSGKSVFHTIHAELGDSMVEFGTTKATERLKRL 183

Query: 183 VYGSNSDMESQKEKQWWDEFPSEFPSDNWD 213
           VYGSNSD+E QKEKQWW+EFPSE    NWD
Sbjct: 184 VYGSNSDVELQKEKQWWEEFPSE----NWD 204

[Close]

BLAST of Spg008010 vs. TAIR 10
Match: AT4G20970.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 3.6e-24
Identity = 72/183 (39.34%), Postives = 115/183 (62.84%), Query Frame = 0


Query: 9   ASPSSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELE 68
           ++   +  V+R+ VEKNRR QMK+L S+L SLLP+  S E  LTLPDQ+DEA  YIK+L+
Sbjct: 6   SNTGQSRSVDRKTVEKNRRMQMKSLYSELISLLPHHSSTE-PLTLPDQLDEAANYIKKLQ 65

Query: 69  KKVNSAKEKKEKLQGKN--KSFLNMDSSSSSSSSSAS-----PQLKIHQMGRSLEIILSS 128
             V   +E+K  L      +   ++ SSS SSS   S     P+++I + G    I L +
Sbjct: 66  VNVEKKRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVT 125

Query: 129 GLDNQYLFCETIRILQEE-GTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLK 184
            L+++++FCE IR+L EE G E+ +A +S+  ++VFHTLH ++ + +     +++ ERL+
Sbjct: 126 SLEHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEHDYG-ARSQIPERLE 185

[Close]

BLAST of Spg008010 vs. TAIR 10
Match: AT1G10585.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 8.7e-10
Identity = 50/175 (28.57%), Postives = 95/175 (54.29%), Query Frame = 0


Query: 12  SSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELEKKV 71
           SS+ + +R + EK+RR +MK+L S L+S +    S    L +P  ID+A  Y+ +L++ V
Sbjct: 12  SSSLREQRNLREKDRRMRMKHLFSILSSHV----SPTRKLPVPHLIDQATSYMIQLKENV 71

Query: 72  NSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILSSGLD-NQYLFC 131
           N  KEKK       ++ L  +  +    S   P+L I     ++E+ L   L+  + +  
Sbjct: 72  NYLKEKK-------RTLLQGELGNLYEGSFLLPKLSIRSRDSTIEMNLIMDLNMKRVMLH 131

Query: 132 ETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRLVYG 186
           E + I +EEG +V++A+     +   +T+ AQ   S +  + +++ ER+++++YG
Sbjct: 132 ELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRIEERVRKIIYG 175

[Close]

BLAST of Spg008010 vs. TAIR 10
Match: AT1G10586.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 47/173 (27.17%), Postives = 93/173 (53.76%), Query Frame = 0


Query: 12  SSAAKVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELEKKV 71
           +S+ + +R + EK RR +MK+L S L+S +    S    L +P  ID+A+ Y+ +L++KV
Sbjct: 12  ASSLREQRNLREKERRMRMKHLFSILSSHV----SPTRRLPVPQLIDQAVSYMIQLKEKV 71

Query: 72  NSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQMGRSLEIILSSGLDNQ-YLFC 131
           N   E K ++ G        +  + S  SS  P+L I  +   +E+ L   L+ +  +  
Sbjct: 72  NYLNEMKRRMLGG-------EVKNRSEGSSLLPKLSIRSLDSIIEMNLVMDLNMKGVMLH 131

Query: 132 ETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRLV 184
           + + + +EEG +V++A+     +  F+T+ AQ     +  + +++ ERL+ ++
Sbjct: 132 KLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDPSRIEERLRDII 173

[Close]

BLAST of Spg008010 vs. TAIR 10
Match: AT5G51780.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 53.1 bits (126), Expect = 3.1e-07
Identity = 48/172 (27.91%), Postives = 87/172 (50.58%), Query Frame = 0


Query: 16  KVERRVVEKNRRNQMKNLCSQLNSLLPNQDSKEISLTLPDQIDEAIKYIKELEKKVNSAK 75
           K+  R  E+ RR +M +L + L SLLP    K    T  DQ++EA+ YIK L++K+    
Sbjct: 3   KMMHRETERQRRQEMASLYASLRSLLPLHFIKGKRST-SDQVNEAVNYIKYLQRKIKELS 62

Query: 76  EKKEKLQGKNKSFL----NMDSSSSSSSSSASPQLKIHQMGRSLEIILSSG-LDNQYLFC 135
            +++ L   ++  L    N D        S    + + Q    +EI+LSS     Q  F 
Sbjct: 63  VRRDDLMVLSRGSLLGSSNGDFKEDVEMISGKNHVVVRQCLVGVEIMLSSRCCGGQPRFS 122

Query: 136 ETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVEFEAAKVTERLKRL 183
             +++L E G  ++N+  S+  + + +T+ A++ D  +  + A++ +RL R+
Sbjct: 123 SVLQVLSEYGLCLLNSISSIVDDRLVYTIQAEVNDMALMIDLAELEKRLIRM 173

[Close]

BLAST of Spg008010 vs. TAIR 10
Match: AT1G10586.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.3e-05
Identity = 34/134 (25.37%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 51  LTLPDQIDEAIKYIKELEKKVNSAKEKKEKLQGKNKSFLNMDSSSSSSSSSASPQLKIHQ 110
           L +P  ID+A+ Y+ +L++KVN   E K ++ G        +  + S  SS  P+L I  
Sbjct: 6   LPVPQLIDQAVSYMIQLKEKVNYLNEMKRRMLGG-------EVKNRSEGSSLLPKLSIRS 65

Query: 111 MGRSLEIILSSGLDNQ-YLFCETIRILQEEGTEVVNASFSVSGNSVFHTLHAQLGDSEVE 170
           +   +E+ L   L+ +  +  + + + +EEG +V++A+     +  F+T+ AQ     + 
Sbjct: 66  LDSIIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIG 125

Query: 171 FEAAKVTERLKRLV 184
            + +++ ERL+ ++
Sbjct: 126 IDPSRIEERLRDII 132

The following BLAST results are available for this feature:

BLAST of Spg008010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888628.1	2.0e-88	83.96	transcription factor bHLH162-like [Benincasa hispida]	[more]
XP_022930718.1	3.7e-87	86.83	transcription factor bHLH162-like [Cucurbita moschata]	[more]
KAG6589225.1	1.0e-84	86.27	Transcription factor basic helix-loop-helix 162, partial [Cucurbita argyrosperma...	[more]
XP_023531689.1	1.7e-84	85.37	transcription factor bHLH162-like [Cucurbita pepo subsp. pepo]	[more]
XP_022988952.1	4.2e-83	83.90	transcription factor bHLH162-like [Cucurbita maxima]	[more]

BLAST of Spg008010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIJ7	5.1e-23	39.34	Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=...	[more]
F4I4E1	1.2e-08	28.57	Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=...	[more]
A2WZ60	1.8e-07	26.92	Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=399...	[more]
Q0JFZ0	1.8e-07	26.92	Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q9XIJ1	2.3e-07	27.17	Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=...	[more]

BLAST of Spg008010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EW58	1.8e-87	86.83	transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111437113 ...	[more]
A0A6J1JEF7	2.1e-83	83.90	transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111486157 PE...	[more]
A0A5D3C788	2.7e-83	79.25	Transcription factor bHLH55-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0K2C9	6.0e-83	79.44	BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G048070 PE=4 S...	[more]
A0A1S3C1C4	2.1e-80	78.10	transcription factor bHLH55-like OS=Cucumis melo OX=3656 GN=LOC103495505 PE=4 SV...	[more]

BLAST of Spg008010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20970.1	3.6e-24	39.34	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10585.1	8.7e-10	28.57	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.1	1.6e-08	27.17	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G51780.1	3.1e-07	27.91	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.2	1.3e-05	25.37	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 57..91
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR13935:SF63	HELIX LOOP HELIX DNA-BINDING DOMAIN PROTEIN	coord: 10..190
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 17..68 e-value: 2.2E-6 score: 27.5
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 14..67 score: 11.782114
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 8..95 e-value: 2.1E-10 score: 42.3
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 12..88
IPR015660	Achaete-scute transcription factor-related	PANTHER	PTHR13935	ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED	coord: 10..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008010.1	Spg008010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:2000112	regulation of cellular macromolecule biosynthetic process
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0090575	RNA polymerase II transcription regulator complex
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000977	RNA polymerase II transcription regulatory region sequence-specific DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity