Spg008005 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008005
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDiacylglycerol O-acyltransferase
Locationscaffold4: 7945229 .. 7949466 (-)
RNA-Seq ExpressionSpg008005
SyntenySpg008005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGACCATGCACGTATGCATTTTATCACAGGCAGTAGCCAAATATGTATACACGACGGCGAAAAATTCAAGCATAAGCATTTCAAATATGATCGGGCCAAATGAGAAAGTGTCTCTCAACGGTCACCCAGCAAAAGGAATGTACTTTTCTGTGGTTGGTATACCTCAGGTACTATGCGCGTTTTATTATTTGGAGAATAATTGCTTGCATCCTGGATATGTAAGGATCGTAAGGCCTGTTTGGTAGGCAATCCGGATTCTGTTTTATGTTTTCAGATTCACTGAATTAAGTGAATATGCGTTTGGCAGATGTGTTTTTGAAGACTGTTTTCAGATTCTGTGATTCAAATAGTGGATATTCTGAAAACAACTTTTTTATGTTTTCAGTGTATCCAGATTTTAAATTTAAAATTTTTTTAAATACATAATTAAATTAAAAATGATAAAAACTTTAATAAATATAGTATTTTCATGTTATAAACTCAATTCATTTTTGTGAAATTAAAATTTGTATAATATAATGTATTATAAATTATATGATATTATAAAATAATAATTATACAAAATTCATAACATATAAAATGCTTATAAATTAATTAATAATAGTTTATATTCAACTTACTACTTTAGTTGATAACTACAAATAGTTTATGGTTTATAAATATATTATAAAAACACATTTTGAACAATTTTTGAAATTGGAATCAAATTTCTGAATCTGCTACCAAACACATATCTGAAAACATGAAATACAACTTTGTTTTCATTGAATTCCTTGTGTTTCAAATTTCTGTTTTCAAATCACCTACCAAGCAGACCCTTAGTTTCTACCTTTCTCGGTTGTCAGGATTTTAATGAAGTTTGTAAGAAAATCCCTATCCACCACTATGCAGCCTATTGTTCTTATTTTTTATTCTTAGAAAAAAACTATTTGGGAGTGTTTTTACAAGCTCAATTTTCAAGAAATTCGTTAGATTTTAGTATAATAGATGAGTGGTTATCACAAAGTTTAAACTAATTCTCAAAACAATAATTTACCTTTTTTCACTTTTTTTATAATTCTTAGGCTCCGTTTAAATAACTGTTTGGTTTTTTAAAATTAAGTTTATAAACACTACTTCCATTTACAAATTTATATGTTTTATTATCTACTTCATTCCTATGGTTTCAAAAACCAAGTCAAGTTTTAAAAACTAAAAAAAATTAGATTTCAAAAACTTGTTTTTATTTTTGGGATTTGGCTAAGAATTCAAATGTGTCCTTAACAAAGATGAAAACCATGGTATAGAAATTTGGAGAAAACAAGCATGATCTTCAAAAACCCAAATTGAACCAAAATGGTTAATATATGTAAGGGACAACTGGACCCCCACATATCTCCACAATAGTATGATATTGTCCACTTTGGGCATAAGCCCTCATGGTTTTTCTTTTGGTTCTCCCCAAAAGACCTCATACGAATGAAGATACTTGTCCTCCCTTATAAGTCCATGATCCTCCCATTATCTAGCCAATGCGGGACTTTGGTCGCACTCTCTAATTCCCAACAATCCTCCCCTCAAACGAAGTACCACCGAGCCTCCCCTCAAATAGTCATCTATCCGTCCAACTCTCTAGGCTTCACCGCTTTACCGAGAGTTCTTGCTCTTATCCAGGATGACTCCTTTTCTCCGAAGTATACCGCCTGCTCAACAGAGCCCAACCACGGATTTTCCCCATGACTATTAAGCTCATAAACTTCTTTGTTCGGCACCAGAGGATTCCACCCGACACGGCTTTCGTCTTGGGGCATGACTCTGATACCACTTGTAAGGGACAACCGGACACCCACATATCTCCACAATGGTATGATATTCTCCACTTTGGGCATAAGCCCTCATGACTTTGTTTTTGGAAAGGCCTCATACCAATGGAGATACTTGTCCTCCCTTATAAACCCATGATCCTCCCATTATCTAGCCAATGTGGGACTTTGGTCGCACTTTCTAATTCCCAAAAACTGGACAATATCATACCATTGTGGAGATATGTGGGTGTTCGGTAGTCCCTTACAAGTGGTATCAGAGTCATGTCCCAGAGGTAGCCATGTCGGGTGGAATCCTCGGGTGCCGAGCAAAGAAGTTGTTGTTGGGTTTTATGCCCTAAAACTCGTAGATAGTGAATGTAAACAAATTTTATACTTGTGTTTAATAAAGTGTTATTTAGTTTCTGCAATAAAATGTTATTGTTAAATATTGCACTATTTGACCCAAATCCAATAAACTAATATCCAAGGTTGTTTGTATGTAACTTGAACTAGTATGTGGGGACATATGGGGATCGATGTTCAAGTAACAACCTAAAAGGTCTATAGTACAAGGATAAGGCTGGGTACCTTATCTTGGTAACACTGTGTGATACGGCCCGCTTTGTATTTGATACAGACGTATTGATCCAAAGCGTCCATGTAGTAGACATGCGAGTGGGGGTATCCTATGCAATGAGTTTGCATAAGACCGAACCGCGAAATAGTAACCGCTAGCTATAACACCGTTAACTGGTTAGGTTTCTATTTCACTAGGATGACCTAGGCAACTTAGTCTGAATCCTGAGTGGATTATGGACTCCTATTCTCGAGGGATTGTCCTTTGATTTGTATGGGTGAGAGTGGCCAGTACGCCGACTCAATAAGCCTACCATTTTGGGGACAAGACCGAGTGGAGAGTTGGGAACATAATCACACAAGATGGAATTCACTCCTTCCCGACTTTTGAGGAAGTAGATGAGTGTTCCCTTAAGTGGTGTCTCCGGGTCTTGAACAAAGGGCCCTACCCTCTCACTGGCCCGAGAGAGGTTTTCTGTTATGGTTGGACCACAAACATGTTGTTCATTAGAGGAGCACTGGTACTTAAGGAAGTAGAGGTAATTCAGGGGTAAAATAGTAATTTGACCCAGCTGGAGTTACGAACACTCGTGAAGGACTAACTTGCTGTTATTGGTCGATATCCGTGGACACAGAAATATTCTGCAGTGAGAAGAGTGTAGTTGTGGGTCTTTAGTGGAGTGACCCACAATTAACGAATGGAGACTAACTTGGTTAATGAGTTTAGCCAATTAGTCTCGTATCGTTGGAGCTTCTGATCTGTAGGTCCATTAGGTCCCACTGGTAGCTCGTAAAGAGACTTGAAGCAAATAAATATAAATTGTAAATTGAAAGTGTTCAATTTATTTGGGAAAACATATAATGTATATTGATGCATTATAATATAAAGTTTATGAAACTTTATAATATAATTTTTTAATTAAATTTCAAAAATTAATTCTTGAGAGAAAATATTTGAAAGAGTTCAAATAAATTTAATATGAATTGGATTCATATTAATTTTAATTTTAATAAAATCCCTTAATTAATTATTTATTAAAATTAGAGACGTTTTCCTATTAGGAAAGGCCTTCTTTTTCTTATTAGGAAAAAGGTCTTGGCCTTTACTATATATATGACCCCTGGGGTGGGGTGAAAGGCATCTCATTGTCTCTCCTTCTCTCAGAGATCTCCCCCAAAAGACTCCCACAAGTCTCCTGCTTCAAGAAGTCTTAGAGTCATACCGGTGTAGCCATAGTGGTGGTGTTCGCACTAGTTTGTGATTATTTCACAAGCAATTGGTGAATTTAATCTGAGTTGTCAACGAGAGTAAATAATTTGTTACTATATAAATTAGATTTAGATCTAATATTTTTGTATGCTTAGCCTTAATATAAATCTAGAATAGTTCTAAGTTTATTGCCAATGTATTGGTATTTTAGATTAAACAAAAAATTAATTACGAATCGGTCCCAATCACGCTTCCGCTTTACGGAATTACGGAATTCCAATCCTTCAGTTGTGAGCCTTGAAGTAGTCATGGAGGTTTGTGGTTAAGCTCTAGGCAGGCAGTATACTCCAGAGAAGAAGAGTTGTCCTGGATAAGAGCAAGAACTCTCGGTAAAGCGGTGAACCAAAAGCAAAGTCATGAGGGCTTATGCCCAAAGTGGACAATATCATACTATTGTGGAGATATGCGGGTGTCCGGTTGTTCCTTAGAAGTGGTATCATTGCCATTCCTCAGGCGTAGCCGTGTCGGGTGGAATCCTCAGGTGTCGAACAAAGAAGTTGTGAGCCTAAGTAGTCATGGGGGAAATCCGTGGTTGAGCTCTAGGCAAGCGGGTATACTCCGGAGAAGAGGGGTTGTCCTGGATAAGAGCAAGAACTCTCGGTAA

mRNA sequence

ATGTTGACCATGCACGTATGCATTTTATCACAGGCAGTAGCCAAATATGTATACACGACGGCGAAAAATTCAAGCATAAGCATTTCAAATATGATCGGGCCAAATGAGAAAGTGTCTCTCAACGGTCACCCAGCAAAAGGAATGTACTTTTCTGTGGTTGGTATACCTCAGAGGATTCCACCCGACACGGCTTTCGTCTTGGGGCATGACTCTGATACCACTTGTAAGGGACAACCGGACACCCACATATCTCCACAATGTCCCTTACAAGTGGTATCAGAGTCATGTCCCAGAGGTAGCCATGTCGGGTGGAATCCTCGGGTGCCGAGCAAAGAAGTTGTTGTTGGGTTTTATGCCCTAAAACTCGTAGATAGTGAATGGCCCTACCCTCTCACTGGCCCGAGAGAGGTTTTCTGTTATGGTTGGACCACAAACATGTTGTTCATTAGAGGAGCACTGGTACTTAAGGAAGTAGAGAGATCTCCCCCAAAAGACTCCCACAAGTCTCCTGCTTCAAGAAGTCTTAGAGTCATACCGGTCTCTAGGCAGGCAGTATACTCCAGAGAAGAAGAGTTGTCCTGGATAAGAGCAAGAACTCTCGGTAAAGCGGTGAACCAAAAGCAAAGTCATGAGGGCTTATGCCCAAAGTGGACAATATCATACTATTGTGGAGATATGCGGGTGTCCGGTTGTTCCTTAGAAGTGGTATCATTGCCATTCCTCAGGCGTAGCCGTGTCGGGTGGAATCCTCAGGTGTCGAACAAAGAAGTTGTGAGCCTAAGTAGTCATGGGGGAAATCCGTGGTTGAGCTCTAGGCAAGCGGGTATACTCCGGAGAAGAGGGGTTGTCCTGGATAAGAGCAAGAACTCTCGGTAA

Coding sequence (CDS)

ATGTTGACCATGCACGTATGCATTTTATCACAGGCAGTAGCCAAATATGTATACACGACGGCGAAAAATTCAAGCATAAGCATTTCAAATATGATCGGGCCAAATGAGAAAGTGTCTCTCAACGGTCACCCAGCAAAAGGAATGTACTTTTCTGTGGTTGGTATACCTCAGAGGATTCCACCCGACACGGCTTTCGTCTTGGGGCATGACTCTGATACCACTTGTAAGGGACAACCGGACACCCACATATCTCCACAATGTCCCTTACAAGTGGTATCAGAGTCATGTCCCAGAGGTAGCCATGTCGGGTGGAATCCTCGGGTGCCGAGCAAAGAAGTTGTTGTTGGGTTTTATGCCCTAAAACTCGTAGATAGTGAATGGCCCTACCCTCTCACTGGCCCGAGAGAGGTTTTCTGTTATGGTTGGACCACAAACATGTTGTTCATTAGAGGAGCACTGGTACTTAAGGAAGTAGAGAGATCTCCCCCAAAAGACTCCCACAAGTCTCCTGCTTCAAGAAGTCTTAGAGTCATACCGGTCTCTAGGCAGGCAGTATACTCCAGAGAAGAAGAGTTGTCCTGGATAAGAGCAAGAACTCTCGGTAAAGCGGTGAACCAAAAGCAAAGTCATGAGGGCTTATGCCCAAAGTGGACAATATCATACTATTGTGGAGATATGCGGGTGTCCGGTTGTTCCTTAGAAGTGGTATCATTGCCATTCCTCAGGCGTAGCCGTGTCGGGTGGAATCCTCAGGTGTCGAACAAAGAAGTTGTGAGCCTAAGTAGTCATGGGGGAAATCCGTGGTTGAGCTCTAGGCAAGCGGGTATACTCCGGAGAAGAGGGGTTGTCCTGGATAAGAGCAAGAACTCTCGGTAA

Protein sequence

MLTMHVCILSQAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRIPPDTAFVLGHDSDTTCKGQPDTHISPQCPLQVVSESCPRGSHVGWNPRVPSKEVVVGFYALKLVDSEWPYPLTGPREVFCYGWTTNMLFIRGALVLKEVERSPPKDSHKSPASRSLRVIPVSRQAVYSREEELSWIRARTLGKAVNQKQSHEGLCPKWTISYYCGDMRVSGCSLEVVSLPFLRRSRVGWNPQVSNKEVVSLSSHGGNPWLSSRQAGILRRRGVVLDKSKNSR
Homology
BLAST of Spg008005 vs. NCBI nr
Match: XP_038886811.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 83.6 bits (205), Expect = 3.1e-12
Identity = 38/50 (76.00%), Postives = 45/50 (90.00%), Query Frame = 0

Query: 10  SQAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           S+A AKYVY T KNSSISISN+IGP EK++L+GHPAKGMYF+VVGIPQ +
Sbjct: 370 SEAAAKYVYKTIKNSSISISNLIGPKEKIALSGHPAKGMYFTVVGIPQSL 419

BLAST of Spg008005 vs. NCBI nr
Match: XP_038888791.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 79.3 bits (194), Expect = 5.9e-11
Identity = 36/49 (73.47%), Postives = 44/49 (89.80%), Query Frame = 0

Query: 11  QAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           +A AKYVY TA+NSSISISNMIGP EK++L GHPAKG+YF+VVG+PQ +
Sbjct: 375 EAAAKYVYKTARNSSISISNMIGPKEKMALLGHPAKGIYFTVVGLPQSL 423

BLAST of Spg008005 vs. NCBI nr
Match: XP_016901702.1 (PREDICTED: O-acyltransferase WSD1-like, partial [Cucumis melo])

HSP 1 Score: 79.0 bits (193), Expect = 7.7e-11
Identity = 35/50 (70.00%), Postives = 43/50 (86.00%), Query Frame = 0

Query: 10  SQAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           S+  +KYVY T KNSS+SISN+IGP EK++L GHPAKGMYF+VVGIPQ +
Sbjct: 368 SEVASKYVYKTIKNSSLSISNLIGPKEKMALTGHPAKGMYFTVVGIPQSL 417

BLAST of Spg008005 vs. NCBI nr
Match: KAA0031540.1 (O-acyltransferase WSD1-like [Cucumis melo var. makuwa] >TYK06993.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 76.6 bits (187), Expect = 3.8e-10
Identity = 34/46 (73.91%), Postives = 41/46 (89.13%), Query Frame = 0

Query: 14  AKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           +KYVY T KNSS+SISN+IGP EK++L GHPAKGMYF+VVGIPQ +
Sbjct: 194 SKYVYKTIKNSSLSISNLIGPKEKMALTGHPAKGMYFTVVGIPQSL 239

BLAST of Spg008005 vs. NCBI nr
Match: XP_004136846.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43650.1 hypothetical protein Csa_017285 [Cucumis sativus])

HSP 1 Score: 76.3 bits (186), Expect = 5.0e-10
Identity = 33/50 (66.00%), Postives = 42/50 (84.00%), Query Frame = 0

Query: 10  SQAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           S+  +KYVY T KNSS+SI+N+IGP EK++L GHP KGMYF+VVGIPQ +
Sbjct: 368 SEVASKYVYKTIKNSSLSITNLIGPKEKMTLTGHPVKGMYFTVVGIPQSL 417

BLAST of Spg008005 vs. ExPASy TrEMBL
Match: A0A1S4E0D6 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495689 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 3.7e-11
Identity = 35/50 (70.00%), Postives = 43/50 (86.00%), Query Frame = 0

Query: 10  SQAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           S+  +KYVY T KNSS+SISN+IGP EK++L GHPAKGMYF+VVGIPQ +
Sbjct: 368 SEVASKYVYKTIKNSSLSISNLIGPKEKMALTGHPAKGMYFTVVGIPQSL 417

BLAST of Spg008005 vs. ExPASy TrEMBL
Match: A0A5A7SLE1 (O-acyltransferase WSD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003090 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-10
Identity = 34/46 (73.91%), Postives = 41/46 (89.13%), Query Frame = 0

Query: 14  AKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           +KYVY T KNSS+SISN+IGP EK++L GHPAKGMYF+VVGIPQ +
Sbjct: 194 SKYVYKTIKNSSLSISNLIGPKEKMALTGHPAKGMYFTVVGIPQSL 239

BLAST of Spg008005 vs. ExPASy TrEMBL
Match: A0A0A0K1W0 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051300 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 2.4e-10
Identity = 33/50 (66.00%), Postives = 42/50 (84.00%), Query Frame = 0

Query: 10  SQAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           S+  +KYVY T KNSS+SI+N+IGP EK++L GHP KGMYF+VVGIPQ +
Sbjct: 368 SEVASKYVYKTIKNSSLSITNLIGPKEKMTLTGHPVKGMYFTVVGIPQSL 417

BLAST of Spg008005 vs. ExPASy TrEMBL
Match: A0A5A7SQF4 (O-acyltransferase WSD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003080 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.0e-08
Identity = 32/47 (68.09%), Postives = 40/47 (85.11%), Query Frame = 0

Query: 11  QAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQ 58
           +A AK VY  A+NSS+SISNMIGP EK++L GHPAKG+YF++ GIPQ
Sbjct: 79  EAAAKCVYKMARNSSLSISNMIGPKEKMALLGHPAKGIYFTIFGIPQ 125

BLAST of Spg008005 vs. ExPASy TrEMBL
Match: A0A6J1HZY6 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.7e-08
Identity = 29/49 (59.18%), Postives = 42/49 (85.71%), Query Frame = 0

Query: 11  QAVAKYVYTTAKNSSISISNMIGPNEKVSLNGHPAKGMYFSVVGIPQRI 60
           +  AK++Y TA+N+S+SISNM+GP EK+++ GHP KG+YF+VVGIPQ +
Sbjct: 369 EVAAKFMYKTAQNTSLSISNMMGPREKMAMCGHPVKGVYFTVVGIPQSL 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886811.13.1e-1276.00O-acyltransferase WSD1-like isoform X1 [Benincasa hispida][more]
XP_038888791.15.9e-1173.47O-acyltransferase WSD1-like [Benincasa hispida][more]
XP_016901702.17.7e-1170.00PREDICTED: O-acyltransferase WSD1-like, partial [Cucumis melo][more]
KAA0031540.13.8e-1073.91O-acyltransferase WSD1-like [Cucumis melo var. makuwa] >TYK06993.1 O-acyltransfe... [more]
XP_004136846.25.0e-1066.00O-acyltransferase WSD1 [Cucumis sativus] >KGN43650.1 hypothetical protein Csa_01... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S4E0D63.7e-1170.00Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495689 PE=4 SV... [more]
A0A5A7SLE11.9e-1073.91O-acyltransferase WSD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A0A0K1W02.4e-1066.00Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051300 PE=4... [more]
A0A5A7SQF41.0e-0868.09O-acyltransferase WSD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A6J1HZY61.7e-0859.18Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 9..57
e-value: 3.5E-10
score: 40.0
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 4..58
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 4..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008005.1Spg008005.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity