Spg007944 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg007944 vs. NCBI nr
Match: XP_022968991.1 (two pore calcium channel protein 1A-like [Cucurbita maxima])

HSP 1 Score: 1295.4 bits (3351), Expect = 0.0e+00
Identity = 665/738 (90.11%), Postives = 685/738 (92.82%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           MEESLL G  SSSSSSRRQIPHVRF+RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD
Sbjct: 1   MEESLLGGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
           QSNFQSAARLYFIFI+FD +WSLNFFALI+LNFFE+PLWCSKYETHSCNDREYFFLGQLP
Sbjct: 61  QSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEEPLWCSKYETHSCNDREYFFLGQLP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           YLTAVESLIYEA TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LY
Sbjct: 121 YLTAVESLIYEATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLY 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
           LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL
Sbjct: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYVIFEDTQQGKI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGV
Sbjct: 241 AYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAF LMDTNNVGFINKEQCL LLEE
Sbjct: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LNRYR+LPK+S+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS
Sbjct: 361 LNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
           IYHS FSKKLK FVRSP FGYAVSFILILNLVAVIIETTLDI NN GQKAWQELEFVFGW
Sbjct: 421 IYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           IYVVEM LKIY+FGF+NYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYL
Sbjct: 481 IYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+VNA
Sbjct: 541 LIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLVNA 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN  L KTDLD+DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ                
Sbjct: 601 GNATLSKTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFI 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 710
                        VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV
Sbjct: 661 SFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 720

BLAST of Spg007944 vs. NCBI nr
Match: XP_008455316.1 (PREDICTED: two pore calcium channel protein 1B isoform X1 [Cucumis melo])

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 666/743 (89.64%), Postives = 685/743 (92.19%), Query Frame = 0

Query: 1   MEESLLAGG-----SSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIP 60
           MEESLL GG     SS SS+SRRQ PHVRF+RRSDAIAYGSAYQRAAALVDLAEDG+GIP
Sbjct: 1   MEESLLGGGGGGGESSGSSTSRRQTPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIP 60

Query: 61  EKILDQSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFF 120
           EKILDQSNFQSAA+LYFIFIKFD IWSLNFFALIVLNFFEKPLWCSKY+THSCNDREYFF
Sbjct: 61  EKILDQSNFQSAAKLYFIFIKFDWIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF 120

Query: 121 LGQLPYLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFL 180
           LGQLPYLTAVESLIYEAVTL+IL+IHTFFPISYEGAQLYW N LNQLKVICIIIL ADFL
Sbjct: 121 LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFL 180

Query: 181 IYALYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLL 240
           +Y LYLSPVAFDYLPFR APYIRV+FFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLL
Sbjct: 181 VYTLYLSPVAFDYLPFRAAPYIRVIFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLL 240

Query: 241 FSSWLAYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLY 300
           FSSWLAYVIFEDTQQGK+IFTSFG TLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLY
Sbjct: 241 FSSWLAYVIFEDTQQGKLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLY 300

Query: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCL 360
           VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAFSLMDTNNVGFINKEQCL
Sbjct: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL 360

Query: 361 ILLEELNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWF 420
           +LLEELNRYRTLPKIS+DDYELIFDELDDS DFKIN+NEF DLCNAIALKFQKEDIPSWF
Sbjct: 361 MLLEELNRYRTLPKISRDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWF 420

Query: 421 EGYPSIYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE 480
           EGYPSIYHS FSKKLK FVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE
Sbjct: 421 EGYPSIYHSSFSKKLKAFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE 480

Query: 481 FVFGWIYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGE 540
           FVFGWIYVVEM LKIYA+GFMNYWRDGQNQFDF++TWIIVIGETITFVAPSGLTFLSNGE
Sbjct: 481 FVFGWIYVVEMALKIYAYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGE 540

Query: 541 WIRYLLIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600
           WIRYLLIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG
Sbjct: 541 WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600

Query: 601 GIVNAGNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ----------- 660
           GIVNAGN  LPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ           
Sbjct: 601 GIVNAGNPTLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS 660

Query: 661 ------------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKT 710
                             VVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTKT
Sbjct: 661 LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKT 720

BLAST of Spg007944 vs. NCBI nr
Match: KAG6572364.1 (Two pore calcium channel protein 1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 665/739 (89.99%), Postives = 685/739 (92.69%), Query Frame = 0

Query: 1    MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
            MEESLL G  SSSSSSRRQIPHVRF+RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD
Sbjct: 553  MEESLLGGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 612

Query: 61   QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWC-SKYETHSCNDREYFFLGQL 120
            QSNFQSAARLYFIFI+FD +WSLNFFALI+LNFFEKPLWC SKYETHSCNDREYFFLGQL
Sbjct: 613  QSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEKPLWCSSKYETHSCNDREYFFLGQL 672

Query: 121  PYLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYAL 180
            PYLTAVESLIYEA TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY L
Sbjct: 673  PYLTAVESLIYEATTLVILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTL 732

Query: 181  YLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW 240
            YLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW
Sbjct: 733  YLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW 792

Query: 241  LAYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLG 300
            LAYVIFEDTQQGKI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLG
Sbjct: 793  LAYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLG 852

Query: 301  VYFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLE 360
            VYFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAF LMDTNNVGFINKEQCL LLE
Sbjct: 853  VYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLE 912

Query: 361  ELNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYP 420
            ELNRYR+LPK+S+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYP
Sbjct: 913  ELNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYP 972

Query: 421  SIYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFG 480
            SIYHS FSKKLK FVRSP FGYAVSFILILNLVAVIIETTLDI NN GQKAWQELEFVFG
Sbjct: 973  SIYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFG 1032

Query: 481  WIYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRY 540
            WIYVVEM LKIY+FGF+NYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRY
Sbjct: 1033 WIYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRY 1092

Query: 541  LLIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVN 600
            LLIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+VN
Sbjct: 1093 LLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLVN 1152

Query: 601  AGNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ--------------- 660
            AGN  L +TDLD+DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ               
Sbjct: 1153 AGNATLSQTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYF 1212

Query: 661  --------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK 710
                          VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK
Sbjct: 1213 ISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK 1272

BLAST of Spg007944 vs. NCBI nr
Match: XP_022952442.1 (two pore calcium channel protein 1A-like [Cucurbita moschata])

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 665/739 (89.99%), Postives = 685/739 (92.69%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           MEESLL G  SSSSSSRRQIPHVRF+RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD
Sbjct: 1   MEESLLGGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWC-SKYETHSCNDREYFFLGQL 120
           QSNFQSAARLYFIFI+FD +WSLNFFALI+LNFFEKPLWC SKYETHSCNDREYFFLGQL
Sbjct: 61  QSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEKPLWCSSKYETHSCNDREYFFLGQL 120

Query: 121 PYLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYAL 180
           PYLTAVESLIYEA TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY L
Sbjct: 121 PYLTAVESLIYEATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTL 180

Query: 181 YLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW 240
           YLSPVAFD LPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW
Sbjct: 181 YLSPVAFDSLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW 240

Query: 241 LAYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLG 300
           LAYVIFEDTQQGKI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLG
Sbjct: 241 LAYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLG 300

Query: 301 VYFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLE 360
           VYFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAF LMDTNNVGFINKEQCL LLE
Sbjct: 301 VYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLE 360

Query: 361 ELNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYP 420
           ELNRYR+LPK+S+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYP
Sbjct: 361 ELNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYP 420

Query: 421 SIYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFG 480
           SIYHS FSKKLK FVRSP FGYAVSFILILNLVAVIIETTLDI NN GQKAWQELEFVFG
Sbjct: 421 SIYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFG 480

Query: 481 WIYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRY 540
           WIYVVEM LKIY+FGF+NYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRY
Sbjct: 481 WIYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRY 540

Query: 541 LLIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVN 600
           LLIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VN
Sbjct: 541 LLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVN 600

Query: 601 AGNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ--------------- 660
           AGN  L KTDLD+DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ               
Sbjct: 601 AGNATLSKTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYF 660

Query: 661 --------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK 710
                         VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK
Sbjct: 661 ISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK 720

BLAST of Spg007944 vs. NCBI nr
Match: XP_022930707.1 (two pore calcium channel protein 1A-like [Cucurbita moschata])

HSP 1 Score: 1285.4 bits (3325), Expect = 0.0e+00
Identity = 662/738 (89.70%), Postives = 688/738 (93.22%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           M+ESLL   SSSSSSSRRQIPHV+FKRRSDAIAYGSAYQRAAALVDLAEDG+GIPEKILD
Sbjct: 1   MDESLLGVESSSSSSSRRQIPHVKFKRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
           QSNFQSAA+LYFIFIKFD+IWS+NFFAL+VLNFFEKPLWCSKY+THSCNDREYFFLGQLP
Sbjct: 61  QSNFQSAAKLYFIFIKFDLIWSVNFFALVVLNFFEKPLWCSKYDTHSCNDREYFFLGQLP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           YLTAVESLIYEAVTL+IL+IHTFFPIS+EGAQLYWKNPLNQLKVIC+IIL ADFLIY LY
Sbjct: 121 YLTAVESLIYEAVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYTLY 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
           LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWL
Sbjct: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYVIFEDTQQGK++FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGV
Sbjct: 241 AYVIFEDTQQGKLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAFSLMDT+NVGFINK+QCLILLEE
Sbjct: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILLEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LNRYRTLPKIS+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPS FE YPS
Sbjct: 361 LNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAYPS 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
           IY+S  SKKLK FVRSP FGYA+SFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW
Sbjct: 421 IYNSVSSKKLKAFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           IYVVEM LKIYAFGFMNYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRYL
Sbjct: 481 IYVVEMALKIYAFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VNA
Sbjct: 541 LIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVNA 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN  L KTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ                
Sbjct: 601 GNPALSKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVYFI 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 710
                        VVAFVLEAFFAELDIESSENGEEQ+QD DSRKDRRRFVGTKTRSRKV
Sbjct: 661 SFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQEQDADSRKDRRRFVGTKTRSRKV 720

BLAST of Spg007944 vs. ExPASy Swiss-Prot
Match: Q75VR1 (Two pore calcium channel protein 1A OS=Nicotiana tabacum OX=4097 GN=TPC1A PE=2 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 5.6e-284
Identity = 496/732 (67.76%), Postives = 597/732 (81.56%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           MEE LL+G SS+S  +RR+I  + F RR DAIA+GSAYQ+AAALVDLAEDGIG+PE+IL+
Sbjct: 1   MEEYLLSGESSNSGRTRRRIGSI-FDRR-DAIAHGSAYQKAAALVDLAEDGIGLPEEILE 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
            ++F+ AA LYFIF +FD +WSLN+ AL+VLNFFEKPLWCSK+   SCN+R+Y++LG+LP
Sbjct: 61  GASFEKAAELYFIFTRFDFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           +LT  ESLI+E VTLL+L+IH  FPISYEG  LYW++ LN++KVI ++IL AD ++Y L+
Sbjct: 121 FLTGAESLIFEGVTLLLLIIHILFPISYEGFNLYWRSLLNRVKVILLLILVADIVVYILF 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
           L+   F YLPFR+APY+RVVFFI+NIR LR+S  ILAGMLGTYLNV+AL  LFLLFSSWL
Sbjct: 181 LAD--FYYLPFRIAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYV FEDT+QGK  FTS+G TLYQMF+LFTTSNNPDVWIPAYK SRWYCLFFVLYVLLGV
Sbjct: 241 AYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKS+L KQ+++ DRLR   L KAFSL+D  N G +N++QC +L EE
Sbjct: 301 YFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLFEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LN+YRTLPKIS DD++ IF ELDD+ DFKIN++EFADLC AI L+FQKED    FE  P+
Sbjct: 361 LNKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPN 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
            YHS  S+KL+ FVR   F Y + F+L++NLVAVIIETTLDI+NN GQ  WQ++EF FGW
Sbjct: 421 FYHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           +YV+EM LK+Y +GF NYWRDGQN+FDF+VTW+IVIGET TFVAP GLTFLSNGEWIRYL
Sbjct: 481 LYVIEMALKVYTYGFENYWRDGQNRFDFVVTWVIVIGETATFVAPDGLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV++YRAF+ATF TLIPSL+PYLGTIFC+LC YC+LG+Q+FGGIVN 
Sbjct: 541 LIARMLRLIRLLMHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIVNT 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN NL +TDL  +DYLLFNFNDYPNGMVTLFN+LVMGNWQVWMQ                
Sbjct: 601 GNPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTAWTYAYFV 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 704
                        +VAFVLEAF AE+D+E+S    + D D +++ +RRR VGTKTRS++V
Sbjct: 661 SFYLISVLWLLNLIVAFVLEAFQAEVDLEASARCVDGD-DKEAKSERRRNVGTKTRSQRV 720

BLAST of Spg007944 vs. ExPASy Swiss-Prot
Match: Q75VR0 (Two pore calcium channel protein 1B OS=Nicotiana tabacum OX=4097 GN=TPC1B PE=2 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 2.8e-283
Identity = 497/738 (67.34%), Postives = 597/738 (80.89%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           MEE LL G SS+S  +RR+   + F RR DAIA+GSAYQ+AAALVDLAEDGIG+PE+IL+
Sbjct: 1   MEEYLLPGESSNSCRTRRRSGSI-FDRR-DAIAHGSAYQKAAALVDLAEDGIGLPEEILE 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
            ++F+ AA LYF+F +FD +WSLN+ AL+VLNFFEKPLWCSK+   SCN+R+Y++LG+LP
Sbjct: 61  GASFEKAAELYFMFTRFDFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           +LT  ESLI+E VTLL+L+IH  FPISYEG  LYW++ LN+LKVI ++IL AD ++Y L 
Sbjct: 121 FLTGAESLIFEGVTLLLLIIHILFPISYEGFNLYWRSLLNRLKVILLLILVADIVVYILL 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
             P  F YLPFR+APY+RVVFFI+NIR LR+S  ILAGMLGTYLNV+AL  LFLLFSSWL
Sbjct: 181 --PADFYYLPFRIAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYV FEDT+QGK  FTS+G TLYQMF+LFTTSNNPDVWIPAYK SRWYCLFFVLYVLLGV
Sbjct: 241 AYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKS+L KQ+++ DRLR   L KAFSL+D  N G +N++QC +L EE
Sbjct: 301 YFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LN+YRTLPKIS DD++ IF+ELDD+ DFKIN+ EFADLC+AI L+FQKED    FE  P+
Sbjct: 361 LNKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPN 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
            YHS  S+KL+ F+R   F Y + F+L++NLVAVIIETTLDI+NN GQ  WQ++EF FGW
Sbjct: 421 FYHSPASEKLRGFIRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           +YV+EM LK+Y +GF NYWRDGQN+FDFIVTW+IVIGET TFVAP  LTFLSNGEWIRYL
Sbjct: 481 LYVIEMALKVYTYGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV++YRAF+ATFLTLIPSLMPYLGTIFC+LC YC+LG+Q+FGGIVN 
Sbjct: 541 LIARMLRLIRLLMHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNT 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN NL +TDL  +DYLLFNFNDYPNGMVTLFN+LVMGNWQVWMQ                
Sbjct: 601 GNPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAYFV 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 710
                        +VAFVLEAF AE+D+E+S    + D D +++++RRR VGTKTRS++V
Sbjct: 661 SFYLISVLWLLNLIVAFVLEAFQAEMDLEASARCVDGD-DKEAKRERRRNVGTKTRSQRV 720

BLAST of Spg007944 vs. ExPASy Swiss-Prot
Match: Q94KI8 (Two pore calcium channel protein 1 OS=Arabidopsis thaliana OX=3702 GN=TPC1 PE=1 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 3.9e-277
Identity = 468/710 (65.92%), Postives = 583/710 (82.11%), Query Frame = 0

Query: 27  RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNFQSAARLYFIFIKFDIIWSLNFF 86
           RRS+AI +G+ +Q+AAALVDLAEDGIG+P +ILDQS+F  +AR YFIF + D+IWSLN+F
Sbjct: 20  RRSEAITHGTPFQKAAALVDLAEDGIGLPVEILDQSSFGESARYYFIFTRLDLIWSLNYF 79

Query: 87  ALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLPYLTAVESLIYEAVTLLILVIHTFFPI 146
           AL+ LNFFE+PLWC K    SC DR+Y++LG+LPYLT  ES+IYE +TL IL++HTFFPI
Sbjct: 80  ALLFLNFFEQPLWCEKNPKPSCKDRDYYYLGELPYLTNAESIIYEVITLAILLVHTFFPI 139

Query: 147 SYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFRVAPYIRVVFFIMNI 206
           SYEG++++W + LN +KV C++IL  D L+  LYLSP+AFD+LPFR+APY+RV+ FI++I
Sbjct: 140 SYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFRIAPYVRVIIFILSI 199

Query: 207 RHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGKIIFTSFGATLYQMF 266
           R LR+++++L+GMLGTYLN+LAL +LFLLF+SW+A+V+FEDTQQG  +FTS+GATLYQMF
Sbjct: 200 RELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGLTVFTSYGATLYQMF 259

Query: 267 ILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSQLAKQISEM 326
           ILFTTSNNPDVWIPAYK+SRW  +FFVLYVL+GVYFVTNLILAVVYDSFK QLAKQ+S M
Sbjct: 260 ILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVYDSFKEQLAKQVSGM 319

Query: 327 DRLRKNILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISKDDYELIFDELDDSH 386
           D++++ +L KAF L+D++  G I+K QC+ L E+L  YRTLPKISK+++ LIFDELDD+ 
Sbjct: 320 DQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLTNYRTLPKISKEEFGLIFDELDDTR 379

Query: 387 DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKVFVRSPKFGYAVSFI 446
           DFKIN +EFADLC AIAL+FQKE++PS FE +P IYHS  S++L+ FVRSP FGYA+SFI
Sbjct: 380 DFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRAFVRSPNFGYAISFI 439

Query: 447 LILNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYAFGFMNYWRDGQNQF 506
           LI+N +AV++ETTLDIE +  QK WQ  EFVFGWIYV+EM LKIY +GF NYWR+G N+F
Sbjct: 440 LIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYTYGFENYWREGANRF 499

Query: 507 DFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLLMHVQQYRAFLATFL 566
           DF+VTW+IVIGET TF+ P   TF SNGEWIRYLL+ARMLRLIRLLM+VQ+YRAF+ATF+
Sbjct: 500 DFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLLMNVQRYRAFIATFI 559

Query: 567 TLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDDDDYLLFNFNDYPNG 626
           TLIPSLMPYLGTIFCVLC+YC++GVQVFGG+VNAGN  L +T+L +DDYLLFNFNDYPNG
Sbjct: 560 TLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKKLFETELAEDDYLLFNFNDYPNG 619

Query: 627 MVTLFNLLVMGNWQVWMQ-----------------------------VVAFVLEAFFAEL 686
           MVTLFNLLVMGNWQVWM+                             VVAFVLEAFF EL
Sbjct: 620 MVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLNLVVAFVLEAFFTEL 679

Query: 687 DIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAELDNKD 708
           D+E  E  + Q QD   +++RRR  G+K+RS++VD LLHHML  EL   +
Sbjct: 680 DLEEEE--KCQGQDSQEKRNRRRSAGSKSRSQRVDTLLHHMLGDELSKPE 727

BLAST of Spg007944 vs. ExPASy Swiss-Prot
Match: Q5QM84 (Two pore calcium channel protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPC1 PE=1 SV=2)

HSP 1 Score: 885.6 bits (2287), Expect = 3.8e-256
Identity = 449/731 (61.42%), Postives = 554/731 (75.79%), Query Frame = 0

Query: 7   AGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNFQS 66
           +GG      SR      +++RRSDA+AYG+ YQ+AAALVDLAEDG+GIPE +L+ + F+ 
Sbjct: 38  SGGGGGWRGSR------QYQRRSDALAYGNRYQKAAALVDLAEDGVGIPEDVLNDTRFER 97

Query: 67  AARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLPYLTAVE 126
           A R YF++++ D +WSLN FALI+LNF EKPLWC  Y  H+C+ R+ +FLGQLPYL+  E
Sbjct: 98  AMRFYFVYLRLDWLWSLNLFALILLNFLEKPLWCRGYSQHACDQRDLYFLGQLPYLSKTE 157

Query: 127 SLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAF 186
           SLIYE +TL+ILV+  F+P+SYEG  L+WKN +N+LKV+ + ILA D L++       AF
Sbjct: 158 SLIYEGLTLVILVMDIFYPLSYEGLNLFWKNTINKLKVLLLFILACDILVF-------AF 217

Query: 187 DYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFE 246
              PFRVAPYIRV F IMNIR LR   + L GM+GTYLNVLAL LLFLLF+SWLAYV FE
Sbjct: 218 SPQPFRVAPYIRVAFLIMNIRELRMCAVTLVGMVGTYLNVLALSLLFLLFASWLAYVTFE 277

Query: 247 DTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNL 306
           DT QGK +F+S+G TLYQMFILFTTSNNPDVW+PAYK+SRW  LFF++YVLLGVYF+TNL
Sbjct: 278 DTPQGKTVFSSYGTTLYQMFILFTTSNNPDVWVPAYKSSRWSSLFFIVYVLLGVYFLTNL 337

Query: 307 ILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEELNRYRT 366
           ILAV+YDSFK QLAKQ+S+ D  RK+IL KAF ++D    G++NKEQCL LL+ELN+YR+
Sbjct: 338 ILAVIYDSFKEQLAKQVSQADCTRKSILEKAFGIIDATGQGYLNKEQCLSLLDELNKYRS 397

Query: 367 LPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDF 426
           LPK S++D+ELIF ELD S DFK+   EFA LCN IA+KFQKE  PS+ E YPS YHS  
Sbjct: 398 LPKTSREDFELIFAELDQSGDFKVTSEEFATLCNTIAIKFQKEPPPSYLEKYPSFYHSAL 457

Query: 427 SKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEM 486
            + LK FVRSP F Y V F+L++NLVAVIIETTLDIEN+  QK WQE+EFVFGWIYV+EM
Sbjct: 458 CEWLKSFVRSPLFEYIVIFVLLMNLVAVIIETTLDIENSSSQKVWQEVEFVFGWIYVIEM 517

Query: 487 GLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARML 546
            LKI++ GF  YW +GQN+FDF++TW I IGET+TF  PS L+FLSNGEWIRYLL+ RML
Sbjct: 518 ALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLSFLSNGEWIRYLLLGRML 577

Query: 547 RLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLP 606
           RL R+L+ V+++RAF+ATF TL+ SLMPYLG +FC LC+YC+LG+Q+FGGIV AGN  L 
Sbjct: 578 RLTRILLQVRRFRAFVATFFTLMSSLMPYLGIVFCTLCIYCSLGLQIFGGIVYAGNPTLE 637

Query: 607 KTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------------- 666
           +TDL  +DYLLFNFNDYP+GMVTLFNLLVMGNWQ WM+                      
Sbjct: 638 ETDLFSNDYLLFNFNDYPSGMVTLFNLLVMGNWQAWMESYRQLTGSYWSLIYFVSFYLIS 697

Query: 667 -------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHH 709
                  +VAFVLEAFFAE+++E     + QD   + R +RRR V  +T+   VDILLHH
Sbjct: 698 VLLLLNLIVAFVLEAFFAEMELEKDGEADIQDPTLEGR-NRRRSVRVRTKGTMVDILLHH 754

BLAST of Spg007944 vs. ExPASy Swiss-Prot
Match: Q6S5H8 (Two pore calcium channel protein 1 OS=Hordeum vulgare OX=4513 GN=TPC1 PE=2 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 1.0e-245
Identity = 433/736 (58.83%), Postives = 544/736 (73.91%), Query Frame = 0

Query: 2   EESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQ 61
           E  L + GS   S         +++RRSDA+A+G  YQ+AAALVDLAEDG+GIPE +L+ 
Sbjct: 15  ERGLASSGSRRLSDGGGGQGSRKYRRRSDALAHGDRYQKAAALVDLAEDGVGIPEDVLND 74

Query: 62  SNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLPY 121
           + F  A   YF++++ D +WSLN FALI+LNF EKPLWC K   H+C+ R+ +FLGQLPY
Sbjct: 75  TRFGRAMSFYFVYLRLDWLWSLNIFALILLNFLEKPLWCRKDALHACDQRDMYFLGQLPY 134

Query: 122 LTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALYL 181
            +  ESLIYE +TL+ILV+    P+SYEG  ++W++  N+LK++ + ILA D L++    
Sbjct: 135 FSKTESLIYEGLTLVILVMEILCPLSYEGLNIFWRSTTNKLKILLLFILACDILVF---- 194

Query: 182 SPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLA 241
              AF   PFR+APYIRVVF IM IR LR   I LAG++GTYLNVLAL LLFLLF+SWLA
Sbjct: 195 ---AFSSQPFRLAPYIRVVFLIMTIRELRMCAITLAGLIGTYLNVLALSLLFLLFASWLA 254

Query: 242 YVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVY 301
           YV FEDT QGK IF+S+G TLYQMF+LFTTSNNPDVW+PAYK SRWY LFF++YVLLGVY
Sbjct: 255 YVTFEDTPQGKTIFSSYGVTLYQMFVLFTTSNNPDVWVPAYKISRWYSLFFIVYVLLGVY 314

Query: 302 FVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEEL 361
           F+TNLILAV+YDSFK Q AKQ+ ++D +RKNIL KAF L+DTN  G++++EQC+ LL EL
Sbjct: 315 FLTNLILAVIYDSFKEQFAKQLVQVDAIRKNILQKAFELIDTNTRGYLDREQCISLLNEL 374

Query: 362 NRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSI 421
           N+YR+LPK S++D+ELIF ELD S DFK+   EFADLCN IA+KFQKE  PS+ E +P  
Sbjct: 375 NKYRSLPKTSREDFELIFAELDRSGDFKVTSEEFADLCNTIAIKFQKEPPPSYLEKFP-F 434

Query: 422 YHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGWI 481
           YHS    +LK FVRS  F Y + F+L++NLVAVIIETTLDIEN+  Q+ WQE+EF  GWI
Sbjct: 435 YHSPVCGRLKSFVRSRTFEYIIVFVLLINLVAVIIETTLDIENSSSQETWQEVEFFLGWI 494

Query: 482 YVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLL 541
           YV EM LKI++ GF  YW +GQN+FDF++TW I IGET+TF  PS L FLSNGEWIRYLL
Sbjct: 495 YVAEMALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLPFLSNGEWIRYLL 554

Query: 542 IARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAG 601
           + R+LRL R+L+ VQ++R F+ATF TL+ SLMPYLG +FC+LC+YC+LG+Q+FGGIV AG
Sbjct: 555 LGRVLRLTRILLQVQRFRVFVATFFTLMSSLMPYLGIVFCILCMYCSLGLQIFGGIVYAG 614

Query: 602 NVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ----------------- 661
           N  L +TDL  +DYLLFNFNDYP+GMVTLFNLLVMGNWQVWM+                 
Sbjct: 615 NPTLEETDLFSNDYLLFNFNDYPSGMVTLFNLLVMGNWQVWMESYWQLTGSSWSLIYFVS 674

Query: 662 ------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVD 709
                       +VAFVLEAFFAE+++E  E  + Q       K +RR +  +++   VD
Sbjct: 675 FYLISILLLLNLIVAFVLEAFFAEMELEKGEEVDIQSPTSGGIK-KRRSMRVRSKGTMVD 734

BLAST of Spg007944 vs. ExPASy TrEMBL
Match: A0A6J1HWG3 (two pore calcium channel protein 1A-like OS=Cucurbita maxima OX=3661 GN=LOC111468128 PE=3 SV=1)

HSP 1 Score: 1295.4 bits (3351), Expect = 0.0e+00
Identity = 665/738 (90.11%), Postives = 685/738 (92.82%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           MEESLL G  SSSSSSRRQIPHVRF+RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD
Sbjct: 1   MEESLLGGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
           QSNFQSAARLYFIFI+FD +WSLNFFALI+LNFFE+PLWCSKYETHSCNDREYFFLGQLP
Sbjct: 61  QSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEEPLWCSKYETHSCNDREYFFLGQLP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           YLTAVESLIYEA TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY LY
Sbjct: 121 YLTAVESLIYEATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTLY 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
           LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL
Sbjct: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYVIFEDTQQGKI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLGV
Sbjct: 241 AYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAF LMDTNNVGFINKEQCL LLEE
Sbjct: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LNRYR+LPK+S+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS
Sbjct: 361 LNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
           IYHS FSKKLK FVRSP FGYAVSFILILNLVAVIIETTLDI NN GQKAWQELEFVFGW
Sbjct: 421 IYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           IYVVEM LKIY+FGF+NYWRDGQNQFDF+VTWIIVIGETITFVAPSGLTFLSNGEWIRYL
Sbjct: 481 IYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+VNA
Sbjct: 541 LIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLVNA 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN  L KTDLD+DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ                
Sbjct: 601 GNATLSKTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYFI 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 710
                        VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV
Sbjct: 661 SFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 720

BLAST of Spg007944 vs. ExPASy TrEMBL
Match: A0A1S3C1V7 (two pore calcium channel protein 1B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495512 PE=3 SV=1)

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 666/743 (89.64%), Postives = 685/743 (92.19%), Query Frame = 0

Query: 1   MEESLLAGG-----SSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIP 60
           MEESLL GG     SS SS+SRRQ PHVRF+RRSDAIAYGSAYQRAAALVDLAEDG+GIP
Sbjct: 1   MEESLLGGGGGGGESSGSSTSRRQTPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIP 60

Query: 61  EKILDQSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFF 120
           EKILDQSNFQSAA+LYFIFIKFD IWSLNFFALIVLNFFEKPLWCSKY+THSCNDREYFF
Sbjct: 61  EKILDQSNFQSAAKLYFIFIKFDWIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF 120

Query: 121 LGQLPYLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFL 180
           LGQLPYLTAVESLIYEAVTL+IL+IHTFFPISYEGAQLYW N LNQLKVICIIIL ADFL
Sbjct: 121 LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFL 180

Query: 181 IYALYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLL 240
           +Y LYLSPVAFDYLPFR APYIRV+FFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLL
Sbjct: 181 VYTLYLSPVAFDYLPFRAAPYIRVIFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLL 240

Query: 241 FSSWLAYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLY 300
           FSSWLAYVIFEDTQQGK+IFTSFG TLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLY
Sbjct: 241 FSSWLAYVIFEDTQQGKLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLY 300

Query: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCL 360
           VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAFSLMDTNNVGFINKEQCL
Sbjct: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL 360

Query: 361 ILLEELNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWF 420
           +LLEELNRYRTLPKIS+DDYELIFDELDDS DFKIN+NEF DLCNAIALKFQKEDIPSWF
Sbjct: 361 MLLEELNRYRTLPKISRDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWF 420

Query: 421 EGYPSIYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE 480
           EGYPSIYHS FSKKLK FVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE
Sbjct: 421 EGYPSIYHSSFSKKLKAFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE 480

Query: 481 FVFGWIYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGE 540
           FVFGWIYVVEM LKIYA+GFMNYWRDGQNQFDF++TWIIVIGETITFVAPSGLTFLSNGE
Sbjct: 481 FVFGWIYVVEMALKIYAYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGE 540

Query: 541 WIRYLLIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600
           WIRYLLIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG
Sbjct: 541 WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600

Query: 601 GIVNAGNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ----------- 660
           GIVNAGN  LPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ           
Sbjct: 601 GIVNAGNPTLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS 660

Query: 661 ------------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKT 710
                             VVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTKT
Sbjct: 661 LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKT 720

BLAST of Spg007944 vs. ExPASy TrEMBL
Match: A0A6J1GKF4 (two pore calcium channel protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111455128 PE=3 SV=1)

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 665/739 (89.99%), Postives = 685/739 (92.69%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           MEESLL G  SSSSSSRRQIPHVRF+RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD
Sbjct: 1   MEESLLGGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWC-SKYETHSCNDREYFFLGQL 120
           QSNFQSAARLYFIFI+FD +WSLNFFALI+LNFFEKPLWC SKYETHSCNDREYFFLGQL
Sbjct: 61  QSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEKPLWCSSKYETHSCNDREYFFLGQL 120

Query: 121 PYLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYAL 180
           PYLTAVESLIYEA TL+IL IHTFFPISYEGAQLYWKNPLNQLKV+C IIL ADF+IY L
Sbjct: 121 PYLTAVESLIYEATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYTL 180

Query: 181 YLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW 240
           YLSPVAFD LPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW
Sbjct: 181 YLSPVAFDSLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSW 240

Query: 241 LAYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLG 300
           LAYVIFEDTQQGKI+FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLLG
Sbjct: 241 LAYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLLG 300

Query: 301 VYFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLE 360
           VYFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAF LMDTNNVGFINKEQCL LLE
Sbjct: 301 VYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALLE 360

Query: 361 ELNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYP 420
           ELNRYR+LPK+S+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPSWFEGYP
Sbjct: 361 ELNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGYP 420

Query: 421 SIYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFG 480
           SIYHS FSKKLK FVRSP FGYAVSFILILNLVAVIIETTLDI NN GQKAWQELEFVFG
Sbjct: 421 SIYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVFG 480

Query: 481 WIYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRY 540
           WIYVVEM LKIY+FGF+NYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRY
Sbjct: 481 WIYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRY 540

Query: 541 LLIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVN 600
           LLIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VN
Sbjct: 541 LLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVN 600

Query: 601 AGNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ--------------- 660
           AGN  L KTDLD+DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ               
Sbjct: 601 AGNATLSKTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVYF 660

Query: 661 --------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK 710
                         VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK
Sbjct: 661 ISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRK 720

BLAST of Spg007944 vs. ExPASy TrEMBL
Match: A0A6J1ERC4 (two pore calcium channel protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111437105 PE=3 SV=1)

HSP 1 Score: 1285.4 bits (3325), Expect = 0.0e+00
Identity = 662/738 (89.70%), Postives = 688/738 (93.22%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           M+ESLL   SSSSSSSRRQIPHV+FKRRSDAIAYGSAYQRAAALVDLAEDG+GIPEKILD
Sbjct: 1   MDESLLGVESSSSSSSRRQIPHVKFKRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
           QSNFQSAA+LYFIFIKFD+IWS+NFFAL+VLNFFEKPLWCSKY+THSCNDREYFFLGQLP
Sbjct: 61  QSNFQSAAKLYFIFIKFDLIWSVNFFALVVLNFFEKPLWCSKYDTHSCNDREYFFLGQLP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           YLTAVESLIYEAVTL+IL+IHTFFPIS+EGAQLYWKNPLNQLKVIC+IIL ADFLIY LY
Sbjct: 121 YLTAVESLIYEAVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYTLY 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
           LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWL
Sbjct: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYVIFEDTQQGK++FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGV
Sbjct: 241 AYVIFEDTQQGKLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAFSLMDT+NVGFINK+QCLILLEE
Sbjct: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILLEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LNRYRTLPKIS+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPS FE YPS
Sbjct: 361 LNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAYPS 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
           IY+S  SKKLK FVRSP FGYA+SFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW
Sbjct: 421 IYNSVSSKKLKAFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           IYVVEM LKIYAFGFMNYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRYL
Sbjct: 481 IYVVEMALKIYAFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VNA
Sbjct: 541 LIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVNA 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN  L KTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ                
Sbjct: 601 GNPALSKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVYFI 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 710
                        VVAFVLEAFFAELDIESSENGEEQ+QD DSRKDRRRFVGTKTRSRKV
Sbjct: 661 SFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQEQDADSRKDRRRFVGTKTRSRKV 720

BLAST of Spg007944 vs. ExPASy TrEMBL
Match: A0A6J1JDS0 (two pore calcium channel protein 1A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485952 PE=3 SV=1)

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 661/738 (89.57%), Postives = 685/738 (92.82%), Query Frame = 0

Query: 1   MEESLLAGGSSSSSSSRRQIPHVRFKRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILD 60
           M+ESLL   S SSSSSRRQIPHV+FKRRSDAIAYGSAYQRAAALVDLAEDG+GIPEKILD
Sbjct: 1   MDESLLGVESGSSSSSRRQIPHVKFKRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKILD 60

Query: 61  QSNFQSAARLYFIFIKFDIIWSLNFFALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLP 120
           QSNFQSAA+LYFIFIKFD+IWS+NFFALIVLNFFEKPLWCSKY+THSCNDREYFFLGQLP
Sbjct: 61  QSNFQSAAKLYFIFIKFDLIWSVNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQLP 120

Query: 121 YLTAVESLIYEAVTLLILVIHTFFPISYEGAQLYWKNPLNQLKVICIIILAADFLIYALY 180
           YLTAVESLIYEAVTL+IL+IHTFFPIS+EGAQLYWKNPLNQLKVIC+IIL ADFLIY LY
Sbjct: 121 YLTAVESLIYEAVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYTLY 180

Query: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSSWL 240
           LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLAL  LFLLFSSWL
Sbjct: 181 LSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSSWL 240

Query: 241 AYVIFEDTQQGKIIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGV 300
           AYVIFEDT+QGK++FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLLGV
Sbjct: 241 AYVIFEDTEQGKLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGV 300

Query: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKNILAKAFSLMDTNNVGFINKEQCLILLEE 360
           YFVTNLILAVVYDSFKSQLAKQISEMDRLRK+ILAKAFSLMDT+NVGFINK+QCLILLEE
Sbjct: 301 YFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILLEE 360

Query: 361 LNRYRTLPKISKDDYELIFDELDDSHDFKINMNEFADLCNAIALKFQKEDIPSWFEGYPS 420
           LNRYRTLPKIS+DDYELIFDELDDS DFKINMNEFADLCNAIALKFQKEDIPS FE YP 
Sbjct: 361 LNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAYPL 420

Query: 421 IYHSDFSKKLKVFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480
           IY S  SKKLK FVRSP FGYA+SFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW
Sbjct: 421 IYDSVSSKKLKAFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGW 480

Query: 481 IYVVEMGLKIYAFGFMNYWRDGQNQFDFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYL 540
           IYVVEM LKIYAFGFMNYWRDGQNQFDF+VTWIIVIGET+TFVAPSGLTFLSNGEWIRYL
Sbjct: 481 IYVVEMALKIYAFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIRYL 540

Query: 541 LIARMLRLIRLLMHVQQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNA 600
           LIARMLRLIRLLMHV+QYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+VNA
Sbjct: 541 LIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLVNA 600

Query: 601 GNVNLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQ---------------- 660
           GN  L KTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ                
Sbjct: 601 GNPALSKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVYFI 660

Query: 661 -------------VVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKV 710
                        VVAFVLEAFFAELDIESSENGEEQDQD DSRKDRRRFVGTKTRSRKV
Sbjct: 661 SFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDADSRKDRRRFVGTKTRSRKV 720

BLAST of Spg007944 vs. TAIR 10
Match: AT4G03560.1 (two-pore channel 1 )

HSP 1 Score: 955.3 bits (2468), Expect = 2.8e-278
Identity = 468/710 (65.92%), Postives = 583/710 (82.11%), Query Frame = 0

Query: 27  RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNFQSAARLYFIFIKFDIIWSLNFF 86
           RRS+AI +G+ +Q+AAALVDLAEDGIG+P +ILDQS+F  +AR YFIF + D+IWSLN+F
Sbjct: 20  RRSEAITHGTPFQKAAALVDLAEDGIGLPVEILDQSSFGESARYYFIFTRLDLIWSLNYF 79

Query: 87  ALIVLNFFEKPLWCSKYETHSCNDREYFFLGQLPYLTAVESLIYEAVTLLILVIHTFFPI 146
           AL+ LNFFE+PLWC K    SC DR+Y++LG+LPYLT  ES+IYE +TL IL++HTFFPI
Sbjct: 80  ALLFLNFFEQPLWCEKNPKPSCKDRDYYYLGELPYLTNAESIIYEVITLAILLVHTFFPI 139

Query: 147 SYEGAQLYWKNPLNQLKVICIIILAADFLIYALYLSPVAFDYLPFRVAPYIRVVFFIMNI 206
           SYEG++++W + LN +KV C++IL  D L+  LYLSP+AFD+LPFR+APY+RV+ FI++I
Sbjct: 140 SYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFRIAPYVRVIIFILSI 199

Query: 207 RHLRESIIILAGMLGTYLNVLALGLLFLLFSSWLAYVIFEDTQQGKIIFTSFGATLYQMF 266
           R LR+++++L+GMLGTYLN+LAL +LFLLF+SW+A+V+FEDTQQG  +FTS+GATLYQMF
Sbjct: 200 RELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGLTVFTSYGATLYQMF 259

Query: 267 ILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSQLAKQISEM 326
           ILFTTSNNPDVWIPAYK+SRW  +FFVLYVL+GVYFVTNLILAVVYDSFK QLAKQ+S M
Sbjct: 260 ILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVYDSFKEQLAKQVSGM 319

Query: 327 DRLRKNILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISKDDYELIFDELDDSH 386
           D++++ +L KAF L+D++  G I+K QC+ L E+L  YRTLPKISK+++ LIFDELDD+ 
Sbjct: 320 DQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLTNYRTLPKISKEEFGLIFDELDDTR 379

Query: 387 DFKINMNEFADLCNAIALKFQKEDIPSWFEGYPSIYHSDFSKKLKVFVRSPKFGYAVSFI 446
           DFKIN +EFADLC AIAL+FQKE++PS FE +P IYHS  S++L+ FVRSP FGYA+SFI
Sbjct: 380 DFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRAFVRSPNFGYAISFI 439

Query: 447 LILNLVAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMGLKIYAFGFMNYWRDGQNQF 506
           LI+N +AV++ETTLDIE +  QK WQ  EFVFGWIYV+EM LKIY +GF NYWR+G N+F
Sbjct: 440 LIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYTYGFENYWREGANRF 499

Query: 507 DFIVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLLMHVQQYRAFLATFL 566
           DF+VTW+IVIGET TF+ P   TF SNGEWIRYLL+ARMLRLIRLLM+VQ+YRAF+ATF+
Sbjct: 500 DFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLLMNVQRYRAFIATFI 559

Query: 567 TLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNVNLPKTDLDDDDYLLFNFNDYPNG 626
           TLIPSLMPYLGTIFCVLC+YC++GVQVFGG+VNAGN  L +T+L +DDYLLFNFNDYPNG
Sbjct: 560 TLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKKLFETELAEDDYLLFNFNDYPNG 619

Query: 627 MVTLFNLLVMGNWQVWMQ-----------------------------VVAFVLEAFFAEL 686
           MVTLFNLLVMGNWQVWM+                             VVAFVLEAFF EL
Sbjct: 620 MVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLNLVVAFVLEAFFTEL 679

Query: 687 DIESSENGEEQDQDGDSRKDRRRFVGTKTRSRKVDILLHHMLSAELDNKD 708
           D+E  E  + Q QD   +++RRR  G+K+RS++VD LLHHML  EL   +
Sbjct: 680 DLEEEE--KCQGQDSQEKRNRRRSAGSKSRSQRVDTLLHHMLGDELSKPE 727