Spg007808 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg007808
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description1-aminocyclopropane-1-carboxylate oxidase 1
Locationscaffold4: 8829894 .. 8831633 (+)
RNA-Seq ExpressionSpg007808
SyntenySpg007808
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATCAAAGCAAAGCCTTATATTACTCAACAGCAACACCCAAAGAGAGATTTTTTACAAACCACTTTTTGTATATCTCTGCAAAAAAATGGCTGTCTTTCCTGTCATCAACTTGGACAACATCAATGGTGATGGAAGAGCCAAGATATTGGAGCAAATTGAAGATGCCTGCCAAAACTGGGGATTCTTTGAGGTGATTCATTTCTTTTACGACAATTTGAATGTACGGTCTTTTAAAGGAAGTAGAGATGTCTTGTCCTCACCTGGTGGTAGAGACTTGGATTTTCTTTACTACCTATTCTTTGTTTTGGTCATTTTTGTAATGGGTTGGGCTTGATTTTGCAGCTGGTGAACCATGGGATAGCACATGAGTTTTTGGACACAGTGGAGAAGATGACAAGAGATCATTACAAGAAGTGTATGGAACAGAGGTTCAAGGAGACTGTGGCTAGCAAGGGCTTAGAGGCTGCACAGGCTGAAGTTAAAGATATGGACTGGGAAAGCACCTTCTTCTTGCGCCATCTCCCTGAATCGAACATGTCTGAGATGTCCGACCTTGAGGAGGACTACAAGTATCATATTGTCCGCTTTGAACTTAAATCTGTGTAGTCATATTTATAAGCTTTCTTGTCTTTTGGGACATTGTTCGCGTCTTTAATACTTTCTAATGTGACGTGCATGTGAATGATATACAGGAAGATTATGAAGGAATTTGCAAAGAAGATAGAGAACCTTGCAGAGGAGCTATTGGACCTGCTATGTGAGAATCTTGGGTTGGAAAAGGGTTACTTGAAGAAGGCTTTCTATGGATCAAAAGGTCCTACATTTGGGACAAAGGTGAGCAACTACCCGCCGTGCCCAAAGCCGGACCTGATCAAGGGTCTCCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGATGACAAGGTCAGTGGCCTACAACTCCTCAAGGATGGCGAATGGATCGACGTGCCCCCAATGCGCCATGCCATCGTCGTCAACCTTGGAGACCAACTCGAGGTATATCATTAAAGAAAAACACTCACACTATAGACTCAAAGTGAAGGAGGAAAGGGAAAAAAAAGAGTCCAACTTTAAACTTTTGAGAATGAAAAAGATTATATAAATAAAAGTTAAAACGTAAAACTTTGCAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCACAGAGTGGTAACTCAGACGAATGGAACTGGCCGAATGTCGATAGCTTCATTCTACAACCCGGGGAGCGATGCCGTGATCTACCCGGCTCCTGCGCTAGTCGAGAAAGAGGCTGAGGAGAAGAATCAAGTGTACCCCAAGTTTGTGTTTGAAGACTACATGAAGCTCTACATAGGTGTGAAGTTTCAGGCCAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCCAATTTGGGTCCATTGGCAACAGCATAAAAGAGAAAACAAGGGGAAAAAAAAACCCACTTTTTTAACAATAAATATTTAAAGGGGTTGTCATTGTGTTGCCTTTTAAAGTGGGTTGTGAGTGACTGAAACTATAGCTTATTGTTATTATTTTAATGAAAAGTTAAAAGCAAACATTATTGTAGTAGAAGAATTTATGTTTGTAGCAACTAGCAAAAGTGTTGTTCCTCATTTTCTTTATAATTAAATTCACTTTCAGTTTAACGGTCCAATGAAGTTTCAATATACTAGAAAAGAACATATTTTGAAGAGAAAGTGTG

mRNA sequence

ATGGCTGTCTTTCCTGTCATCAACTTGGACAACATCAATGGTGATGGAAGAGCCAAGATATTGGAGCAAATTGAAGATGCCTGCCAAAACTGGGGATTCTTTGAGCTGGTGAACCATGGGATAGCACATGAGTTTTTGGACACAGTGGAGAAGATGACAAGAGATCATTACAAGAAGTGTATGGAACAGAGGTTCAAGGAGACTGTGGCTAGCAAGGGCTTAGAGGCTGCACAGGCTGAAGTTAAAGATATGGACTGGGAAAGCACCTTCTTCTTGCGCCATCTCCCTGAATCGAACATGTCTGAGATGTCCGACCTTGAGGAGGACTACAAGAAGATTATGAAGGAATTTGCAAAGAAGATAGAGAACCTTGCAGAGGAGCTATTGGACCTGCTATGTGAGAATCTTGGGTTGGAAAAGGGTTACTTGAAGAAGGCTTTCTATGGATCAAAAGGTCCTACATTTGGGACAAAGGTGAGCAACTACCCGCCGTGCCCAAAGCCGGACCTGATCAAGGGTCTCCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGATGACAAGGTCAGTGGCCTACAACTCCTCAAGGATGGCGAATGGATCGACGTGCCCCCAATGCGCCATGCCATCGTCGTCAACCTTGGAGACCAACTCGAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCACAGAGTGGTAACTCAGACGAATGGAACTGGCCGAATGTCGATAGCTTCATTCTACAACCCGGGGAGCGATGCCGTGATCTACCCGGCTCCTGCGCTAGTCGAGAAAGAGGCTGAGGAGAAGAATCAAGTGTACCCCAAGTTTGTGTTTGAAGACTACATGAAGCTCTACATAGGTGTGAAGTTTCAGGCCAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCCAATTTGGGTCCATTGGCAACAGCATAA

Coding sequence (CDS)

ATGGCTGTCTTTCCTGTCATCAACTTGGACAACATCAATGGTGATGGAAGAGCCAAGATATTGGAGCAAATTGAAGATGCCTGCCAAAACTGGGGATTCTTTGAGCTGGTGAACCATGGGATAGCACATGAGTTTTTGGACACAGTGGAGAAGATGACAAGAGATCATTACAAGAAGTGTATGGAACAGAGGTTCAAGGAGACTGTGGCTAGCAAGGGCTTAGAGGCTGCACAGGCTGAAGTTAAAGATATGGACTGGGAAAGCACCTTCTTCTTGCGCCATCTCCCTGAATCGAACATGTCTGAGATGTCCGACCTTGAGGAGGACTACAAGAAGATTATGAAGGAATTTGCAAAGAAGATAGAGAACCTTGCAGAGGAGCTATTGGACCTGCTATGTGAGAATCTTGGGTTGGAAAAGGGTTACTTGAAGAAGGCTTTCTATGGATCAAAAGGTCCTACATTTGGGACAAAGGTGAGCAACTACCCGCCGTGCCCAAAGCCGGACCTGATCAAGGGTCTCCGAGCCCACACCGACGCCGGTGGCATCATCCTCCTCTTCCAAGATGACAAGGTCAGTGGCCTACAACTCCTCAAGGATGGCGAATGGATCGACGTGCCCCCAATGCGCCATGCCATCGTCGTCAACCTTGGAGACCAACTCGAGGTGATCACAAATGGAAGATACAAAAGTGTGATGCACAGAGTGGTAACTCAGACGAATGGAACTGGCCGAATGTCGATAGCTTCATTCTACAACCCGGGGAGCGATGCCGTGATCTACCCGGCTCCTGCGCTAGTCGAGAAAGAGGCTGAGGAGAAGAATCAAGTGTACCCCAAGTTTGTGTTTGAAGACTACATGAAGCTCTACATAGGTGTGAAGTTTCAGGCCAAGGAGCCAAGATTTGAAGCCATGAAAGCCAATGCCAATTTGGGTCCATTGGCAACAGCATAA

Protein sequence

MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKCMEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKKIENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQTNGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEPRFEAMKANANLGPLATA
Homology
BLAST of Spg007808 vs. NCBI nr
Match: KAA0061654.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa] >TYK05852.1 1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 611.3 bits (1575), Expect = 4.7e-171
Identity = 296/318 (93.08%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NINGDGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPALVEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. NCBI nr
Match: NP_001284392.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Full=1-aminocyclopropane-1-carboxylate oxidase 1; Short=ACC oxidase 1; AltName: Full=Ethylene-forming enzyme; Short=EFE; AltName: Full=PMEL1 [Cucumis melo] >ACY08855.1 ACC oxidase [Cucumis melo] >CAA49553.1 enzyme-forming ethylene [Cucumis melo] >CAA64797.1 ACC oxidase [Cucumis melo] >BAA06526.1 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 608.6 bits (1568), Expect = 3.1e-170
Identity = 295/318 (92.77%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NIN DGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPALVEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. NCBI nr
Match: XP_038887306.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Benincasa hispida])

HSP 1 Score: 608.2 bits (1567), Expect = 4.0e-170
Identity = 293/317 (92.43%), Postives = 308/317 (97.16%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NINGDGRAKILEQIEDACQNWGFFELVNHGI H+FLDTVEKMTRDHYKK 
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHKFLDTVEKMTRDHYKKS 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+SEMSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISEMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEP 300
           +GTGRMSIASFYNPGSDAVI+PAP LVEK+  E+ +VYPKFVFEDYMKLYIGVKFQAKEP
Sbjct: 241 SGTGRMSIASFYNPGSDAVIFPAPMLVEKDQAEEKEVYPKFVFEDYMKLYIGVKFQAKEP 300

Query: 301 RFEAMKANANLGPLATA 318
           RFEAMKANANLGP+ATA
Sbjct: 301 RFEAMKANANLGPMATA 317

BLAST of Spg007808 vs. NCBI nr
Match: BAI39989.1 (ACC oxidase [Cucumis melo])

HSP 1 Score: 607.4 bits (1565), Expect = 6.8e-170
Identity = 294/318 (92.45%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NIN DGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPA+VEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPAVVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. NCBI nr
Match: AEM42994.1 (ACC oxidase [Siraitia grosvenorii])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 292/317 (92.11%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MA FPVINL+NINGDGRAKI+EQ+EDACQNWGFFELVNHGI HEFLDTVEKMTR HYKKC
Sbjct: 1   MANFPVINLENINGDGRAKIMEQLEDACQNWGFFELVNHGIPHEFLDTVEKMTRYHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESN+SE+ DL++DY+KIMKEFAKK
Sbjct: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNISEIPDLDDDYRKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E LAEELLDLLCENLGLE GYLKKAFYGSKGPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLENGYLKKAFYGSKGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNG+YKSVMHRVVTQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGHWIDVPPMRHAIVVNLGDQLEVITNGKYKSVMHRVVTQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEP 300
           NGTGRMSIASFYNPGSDAVI+PAPALVEKEAEE N VYPKFVFEDYMKLY+GVKFQAKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEENNNVYPKFVFEDYMKLYVGVKFQAKEP 300

Query: 301 RFEAMKANANLGPLATA 318
           RFEAMKANANLGP+A A
Sbjct: 301 RFEAMKANANLGPMAIA 317

BLAST of Spg007808 vs. ExPASy Swiss-Prot
Match: Q04644 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 4.0e-173
Identity = 295/318 (92.77%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NIN DGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPALVEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. ExPASy Swiss-Prot
Match: P05116 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=ACO1 PE=2 SV=2)

HSP 1 Score: 532.3 bits (1370), Expect = 3.7e-150
Identity = 254/317 (80.13%), Postives = 287/317 (90.54%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           M  FP+INL+ +NGD RA  +E I+DAC+NWGFFELVNHGI HE +DTVEKMT+ HYKKC
Sbjct: 1   MENFPIINLEKLNGDERANTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           MEQRFKE VASKGLEA QAEV D+DWESTFFLRHLP SN+S++ DL+E+Y+++M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKR 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E LAEELLDLLCENLGLEKGYLK AFYGSKGP FGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKD +WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRV+ QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEP 300
           +GT RMS+ASFYNPGSDAVIYPA  LVEKEAEE  QVYPKFVF+DYMKLY G+KFQAKEP
Sbjct: 241 DGT-RMSLASFYNPGSDAVIYPAKTLVEKEAEESTQVYPKFVFDDYMKLYAGLKFQAKEP 300

Query: 301 RFEAMKANANLGPLATA 318
           RFEAMKA  +  P+A+A
Sbjct: 301 RFEAMKAMES-DPIASA 315

BLAST of Spg007808 vs. ExPASy Swiss-Prot
Match: Q9MB94 (1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 8.1e-150
Identity = 251/320 (78.44%), Postives = 290/320 (90.62%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           M  FP+INL+ +NG+GR   +E+I+DAC+NWGFFELV+HGI  EFLDTVE++T++HY++C
Sbjct: 1   MENFPIINLEGLNGEGRKATMEKIKDACENWGFFELVSHGIPTEFLDTVERLTKEHYRQC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           +EQRFKE VASKGLEA + EV DMDWESTF+LRHLP+SN+SE+ DLE+ Y+ +MKEFA K
Sbjct: 61  LEQRFKELVASKGLEAVKTEVNDMDWESTFYLRHLPKSNISEVPDLEDQYRNVMKEFALK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E LAE+LLDLLCENLGLEKGYLKKAFYG+ GPTFGTKVSNYPPCP P+LIKGLRAHTDA
Sbjct: 121 LEKLAEQLLDLLCENLGLEKGYLKKAFYGTNGPTFGTKVSNYPPCPNPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GG+ILLFQDDKVSGLQLLKDG+WIDVPPMRH+IV+NLGDQLEVITNG+Y+SV HRV+ QT
Sbjct: 181 GGLILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINLGDQLEVITNGKYRSVEHRVIAQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEP 300
           +GT RMSIASFYNPGSDAVIYPAP LVEKEAEEKNQVYPKFVFEDYMKLY G+KFQ KEP
Sbjct: 241 DGT-RMSIASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYAGLKFQPKEP 300

Query: 301 RFEAMKA---NANLGPLATA 318
           RFEAMKA   N +L P+ATA
Sbjct: 301 RFEAMKAVETNISLVPIATA 319

BLAST of Spg007808 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 1.4e-149
Identity = 249/319 (78.06%), Postives = 294/319 (92.16%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           M  FPVI+++ +NG+ RA  +E+I+DAC+NWGFFELVNHGI+HE +DTVE++T++HY KC
Sbjct: 1   MEAFPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           MEQRFKE VA+KGLEA Q+E+ D+DWESTFFLRHLP SN+SE+ DLE+D++K MKEFA+K
Sbjct: 61  MEQRFKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEFAEK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E LAE+LLDLLCEN+GLEKGYLKKAFYGSKGPTFGTKVSNYPPCP+P+LIKGLRAHTDA
Sbjct: 121 LEKLAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQD+KVSGLQLLKDGEWIDVPPM+H+IV+N+GDQLEVITNG+YKSVMHRV+ Q 
Sbjct: 181 GGIILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMHRVIAQP 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEP 300
           +G  RMSIASFYNPGSDAV+YPAPALV+KE +++ QVYPKFVFEDYMKLY G+KFQAKEP
Sbjct: 241 DG-NRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKFQAKEP 300

Query: 301 RFEAMKA--NA-NLGPLAT 317
           RFEAMKA  NA NLGP+AT
Sbjct: 301 RFEAMKAMENAVNLGPIAT 318

BLAST of Spg007808 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 4.0e-149
Identity = 253/321 (78.82%), Postives = 289/321 (90.03%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           M  FP+INL+ +NG  R   +E I+DAC+NWGFFELVNHGI HE +DTVEK+T+ HYKKC
Sbjct: 1   MENFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           MEQRFKE VASKGLEA QAEV D+DWESTFFLRHLP SN+SE+ DL+++Y+++M++FAK+
Sbjct: 61  MEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKR 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E LAEELLDLLCENLGLEKGYLKKAFYGSKGP FGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG+WIDVPPMRH+IVVNLGDQLEVITNG+YKSV+HRV+ QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEK-NQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GT RMS+ASFYNPGSDAVIYPAP LVEKEA+++  QVYPKFVF+DYMKLY G+KFQAKE
Sbjct: 241 DGT-RMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKE 300

Query: 301 PRFEAMK---ANANLGPLATA 318
           PRFEAMK   A+    P+ATA
Sbjct: 301 PRFEAMKAREADVKSDPIATA 320

BLAST of Spg007808 vs. ExPASy TrEMBL
Match: A0A5D3C3Q0 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00060 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 296/318 (93.08%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NINGDGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINGDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPALVEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. ExPASy TrEMBL
Match: D0V4H6 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491357 PE=2 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.5e-170
Identity = 295/318 (92.77%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NIN DGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPALVEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPALVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. ExPASy TrEMBL
Match: C8KIS1 (ACC oxidase OS=Cucumis melo OX=3656 GN=CM-ACO1 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.3e-170
Identity = 294/318 (92.45%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NIN DGRAKILEQIEDACQNWGFFELVNHGI HEFLD VEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINDDGRAKILEQIEDACQNWGFFELVNHGIPHEFLDMVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+S+MSDL+E+YKKIMKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVNDMDWESTFFLRHLPESNISQMSDLDEEYKKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVLTQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEA-EEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGRMSIASFYNPGSDAVIYPAPA+VEK+  EEK +VYPKFVFEDYMKLY+GVKFQAKE
Sbjct: 241 SGTGRMSIASFYNPGSDAVIYPAPAVVEKDQDEEKKEVYPKFVFEDYMKLYLGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANANLGP+ATA
Sbjct: 301 PRFEAMKANANLGPMATA 318

BLAST of Spg007808 vs. ExPASy TrEMBL
Match: A0A097BU17 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.3e-170
Identity = 293/318 (92.14%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MAVFP+INL+NINGDGR KILEQIEDACQNWGFFELVNHGI HEFLDTVEKMTRDHYKKC
Sbjct: 1   MAVFPIINLENINGDGRVKILEQIEDACQNWGFFELVNHGIPHEFLDTVEKMTRDHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKETV SKGLEAAQAEV DMDWESTFFLRHLPESN+SEMSDL+E+YKK+MKEFAKK
Sbjct: 61  MEERFKETVLSKGLEAAQAEVDDMDWESTFFLRHLPESNISEMSDLDEEYKKVMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +ENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA
Sbjct: 121 LENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKD  WIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRV+TQT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDDNWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVITQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVE-KEAEEKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +GTGR+SIASFYNPGSDAVI+PAPALVE  +AEEK +VYPKFVFEDYMKLYIGVKFQAKE
Sbjct: 241 SGTGRLSIASFYNPGSDAVIFPAPALVETDQAEEKKEVYPKFVFEDYMKLYIGVKFQAKE 300

Query: 301 PRFEAMKANANLGPLATA 318
           PRFEAMKANA+LGP+ATA
Sbjct: 301 PRFEAMKANASLGPMATA 318

BLAST of Spg007808 vs. ExPASy TrEMBL
Match: K7NBW2 (ACC oxidase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.3e-170
Identity = 292/317 (92.11%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           MA FPVINL+NINGDGRAKI+EQ+EDACQNWGFFELVNHGI HEFLDTVEKMTR HYKKC
Sbjct: 1   MANFPVINLENINGDGRAKIMEQLEDACQNWGFFELVNHGIPHEFLDTVEKMTRYHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESN+SE+ DL++DY+KIMKEFAKK
Sbjct: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNISEIPDLDDDYRKIMKEFAKK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           +E LAEELLDLLCENLGLE GYLKKAFYGSKGPTFGTKVSNYPPCPKP+LIKGLRAHTDA
Sbjct: 121 LEKLAEELLDLLCENLGLENGYLKKAFYGSKGPTFGTKVSNYPPCPKPELIKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDG WIDVPPMRHAIVVNLGDQLEVITNG+YKSVMHRVVTQ 
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGHWIDVPPMRHAIVVNLGDQLEVITNGKYKSVMHRVVTQA 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEP 300
           NGTGRMSIASFYNPGSDAVI+PAPALVEKEAEE N VYPKFVFEDYMKLY+GVKFQAKEP
Sbjct: 241 NGTGRMSIASFYNPGSDAVIFPAPALVEKEAEENNNVYPKFVFEDYMKLYVGVKFQAKEP 300

Query: 301 RFEAMKANANLGPLATA 318
           RFEAMKANANLGP+A A
Sbjct: 301 RFEAMKANANLGPMAIA 317

BLAST of Spg007808 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 506.5 bits (1303), Expect = 1.5e-143
Identity = 239/323 (73.99%), Postives = 286/323 (88.54%), Query Frame = 0

Query: 1   MAVFPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKC 60
           M  FP+INL+ +NG+ RA  +E+I+DAC+NWGFFE VNHGI+ E LD VEKMT++HYKKC
Sbjct: 1   MESFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKC 60

Query: 61  MEQRFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKK 120
           ME+RFKE++ ++GL++ ++EV D+DWESTF+L+HLP SN+S++ DL++DY+ +MK+FA K
Sbjct: 61  MEERFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGK 120

Query: 121 IENLAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDA 180
           IE L+EELLDLLCENLGLEKGYLKK FYGSK PTFGTKVSNYPPCP PDL+KGLRAHTDA
Sbjct: 121 IEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDA 180

Query: 181 GGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQT 240
           GGIILLFQDDKVSGLQLLKDGEW+DVPP++H+IVVNLGDQLEVITNG+YKSV HRV++QT
Sbjct: 181 GGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQT 240

Query: 241 NGTGRMSIASFYNPGSDAVIYPAPALVEKEAE-EKNQVYPKFVFEDYMKLYIGVKFQAKE 300
           +G GRMSIASFYNPGSD+VI+PAP L+ KEAE EK + YP+FVFEDYMKLY  VKFQAKE
Sbjct: 241 DGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKE 300

Query: 301 PRFEAMKANA-----NLGPLATA 318
           PRFEAMKA       N+GPLATA
Sbjct: 301 PRFEAMKAMETTVANNVGPLATA 323

BLAST of Spg007808 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 464.5 bits (1194), Expect = 6.6e-131
Identity = 215/315 (68.25%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 4   FPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKCMEQ 63
           FPV++L  +NG+ R + +  I +AC+NWGFFE+VNHG+ H+ +D +EKMT+DHYK C EQ
Sbjct: 7   FPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQEQ 66

Query: 64  RFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKKIEN 123
           +F + + SKGL+  + EV+D+DWESTF++RHLP+SN++++SD+ ++Y+  MK+F K++EN
Sbjct: 67  KFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRLEN 126

Query: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183
           LAE+LLDLLCENLGLEKGYLKK F+G+KGPTFGTKVSNYPPCPKP++IKGLRAHTDAGGI
Sbjct: 127 LAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAGGI 186

Query: 184 ILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQTNGT 243
           ILLFQDDKVSGLQLLKDG+WIDVPP+ H+IV+NLGDQLEVITNG+YKSV+HRVVTQ  G 
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQEG- 246

Query: 244 GRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEPRFE 303
            RMS+ASFYNPGSDA I PA +LVEK++E     YP FVF+DYMKLY GVKFQ KEPRF 
Sbjct: 247 NRMSVASFYNPGSDAEISPATSLVEKDSE-----YPSFVFDDYMKLYAGVKFQPKEPRFA 306

Query: 304 AMK---ANANLGPLA 316
           AMK   A   L P A
Sbjct: 307 AMKNASAVTELNPTA 315

BLAST of Spg007808 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 3.6e-129
Identity = 214/315 (67.94%), Postives = 268/315 (85.08%), Query Frame = 0

Query: 4   FPVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKCMEQ 63
           FPVI+L  +NG+ R + +  I+DACQNWGFFELVNHG+ ++ +D +E+MT++HYKK MEQ
Sbjct: 7   FPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQ 66

Query: 64  RFKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKKIEN 123
           +FKE + SKGL+  + EV+D+DWESTF+L HLP+SN+ ++ D+  +Y+  MK+F K++E 
Sbjct: 67  KFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGKRLEI 126

Query: 124 LAEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 183
           LAEELLDLLCENLGLEKGYLKK F+G+ GPTF TK+SNYPPCPKP++IKGLRAHTDAGG+
Sbjct: 127 LAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDAGGL 186

Query: 184 ILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMHRVVTQTNGT 243
           ILLFQDDKVSGLQLLKDG+W+DVPP++H+IV+NLGDQLEVITNG+YKSVMHRV+TQ  G 
Sbjct: 187 ILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQKEG- 246

Query: 244 GRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVKFQAKEPRFE 303
            RMSIASFYNPGSDA I PA +LV+K+++     YP FVF+DYMKLY G+KFQAKEPRFE
Sbjct: 247 NRMSIASFYNPGSDAEISPATSLVDKDSK-----YPSFVFDDYMKLYAGLKFQAKEPRFE 306

Query: 304 AMK---ANANLGPLA 316
           AMK   A A+L P+A
Sbjct: 307 AMKNAEAAADLNPVA 315

BLAST of Spg007808 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 299.3 bits (765), Expect = 3.7e-81
Identity = 144/308 (46.75%), Postives = 208/308 (67.53%), Query Frame = 0

Query: 5   PVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKCMEQR 64
           PVI+   ++G+ R+K +  ++ AC  WGFF + NHGI  E ++ V+KM   HY++ ++++
Sbjct: 12  PVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHLKEK 71

Query: 65  FKETVASKGLEAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAKKIENL 124
           F ++   K L  ++ +  D DWES+FF+ H P SN+ ++ ++ E+  K M E+  ++   
Sbjct: 72  FYQSEMVKAL--SEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEYVCQLHKF 131

Query: 125 AEELLDLLCENLGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPKPDLIKGLRAHTDAGGII 184
           AE L  L+CENLGL++  +  AF G KGP FGTKV+ YP CP+P+L++GLR HTDAGGII
Sbjct: 132 AERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAGGII 191

Query: 185 LLFQDDKVSGLQLLKDGEWIDVPPMR-HAIVVNLGDQLEVITNGRYKSVMHRVVTQTNGT 244
           LL QDD+V GL+  KDG+W+ +PP + + I VN GDQLE+++NGRYKSV+HRV+T  +G+
Sbjct: 192 LLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVKHGS 251

Query: 245 GRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPK-FVFEDYMKLYIGVKFQAKEPRF 304
            R+SIA+FYNP  DA+I PAP L          +YP  + F+DY+KLY   KF  K PR 
Sbjct: 252 -RLSIATFYNPAGDAIISPAPKL----------LYPSGYRFQDYLKLYSTTKFGDKGPRL 306

Query: 305 EAMKANAN 311
           E MK   N
Sbjct: 312 ETMKKMGN 306

BLAST of Spg007808 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-79
Identity = 156/313 (49.84%), Postives = 211/313 (67.41%), Query Frame = 0

Query: 5   PVINLDNINGDGRAKILEQIEDACQNWGFFELVNHGIAHEFLDTVEKMTRDHYKKCMEQR 64
           PVI+   +NG+ R K L +I  AC+ WGFF+LVNHGI  E L+ V+K++ D YK   E+ 
Sbjct: 4   PVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTEREEA 63

Query: 65  FKETVASKGL-----EAAQAEVKDMDWESTFFLRHLPESNMSEMSDLEEDYKKIMKEFAK 124
           FK +   K L     + +  +++++DWE  F L    + N +E      + K+ M E+ +
Sbjct: 64  FKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEW---PSNIKETMGEYRE 123

Query: 125 KIENLAEELLDLLCENLGLEKGYLKKAFY-----GSKGPTFGTKVSNYPPCPKPDLIKGL 184
           ++  LA ++++++ ENLGL KGY+KKAF      G +   FGTKVS+YPPCP P+L+ GL
Sbjct: 124 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 183

Query: 185 RAHTDAGGIILLFQDDKVSGLQLLKDGEWIDVPPMRHAIVVNLGDQLEVITNGRYKSVMH 244
           RAHTDAGG++LLFQDD+  GLQ+LKDGEWIDV P+ +AIV+N GDQ+EV++NGRYKS  H
Sbjct: 184 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 243

Query: 245 RVVTQTNGTGRMSIASFYNPGSDAVIYPAPALVEKEAEEKNQVYPKFVFEDYMKLYIGVK 304
           RV+ +  G  R SIASFYNP   A I PA A+ E+E  EK   YPKFVF DYM +Y   K
Sbjct: 244 RVLAREEG-NRRSIASFYNPSYKAAIGPA-AVAEEEGSEKK--YPKFVFGDYMDVYANQK 303

Query: 305 FQAKEPRFEAMKA 308
           F  KEPRF A+K+
Sbjct: 304 FMPKEPRFLAVKS 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0061654.14.7e-17193.081-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa] >TYK05852... [more]
NP_001284392.13.1e-17092.771-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo] >Q04644.1 RecName: Fu... [more]
XP_038887306.14.0e-17092.431-aminocyclopropane-1-carboxylate oxidase 1 [Benincasa hispida][more]
BAI39989.16.8e-17092.45ACC oxidase [Cucumis melo][more]
AEM42994.11.5e-16992.11ACC oxidase [Siraitia grosvenorii][more]
Match NameE-valueIdentityDescription
Q046444.0e-17392.771-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=ACO1 PE=2... [more]
P051163.7e-15080.131-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=A... [more]
Q9MB948.1e-15078.441-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 ... [more]
P312371.4e-14978.061-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ... [more]
Q085074.0e-14978.821-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P... [more]
Match NameE-valueIdentityDescription
A0A5D3C3Q02.3e-17193.081-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=11946... [more]
D0V4H61.5e-17092.771-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103491... [more]
C8KIS13.3e-17092.45ACC oxidase OS=Cucumis melo OX=3656 GN=CM-ACO1 PE=3 SV=1[more]
A0A097BU177.3e-17092.141-aminocyclopropane-1-carboxylate oxidase 1 OS=Citrullus lanatus OX=3654 PE=2 SV... [more]
K7NBW27.3e-17092.11ACC oxidase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G05010.11.5e-14373.99ethylene-forming enzyme [more]
AT1G62380.16.6e-13168.25ACC oxidase 2 [more]
AT1G12010.13.6e-12967.942-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G19590.13.7e-8146.75ACC oxidase 1 [more]
AT1G77330.11.2e-7949.842-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 103..134
NoneNo IPR availablePANTHERPTHR47990:SF63ACC OXIDASE 2coord: 4..307
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..307
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..305
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 3..316
e-value: 9.2E-102
score: 342.6
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 5..97
e-value: 2.0E-13
score: 51.1
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 159..254
e-value: 1.2E-28
score: 99.5
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 153..254
score: 13.627394

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg007808.1Spg007808.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
molecular_function GO:0009815 1-aminocyclopropane-1-carboxylate oxidase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0046872 metal ion binding