Spg007785 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg007785
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
Locationscaffold4: 8779699 .. 8783821 (+)
RNA-Seq ExpressionSpg007785
SyntenySpg007785
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTCGCCCATAAGCTTGCGTGCCAAGCTGTTTTTGGTTTTTCTGGGAACTGTAATCGGTTTTTTGATACTTCTTCCAATTCGGAAGAATATTTCGTTTTGTTGGGCCATATTGGGCCTGAATATGCATCTCTCCGTTTTTTTTGGGTATGTTGGTTAGTTTATGTCAGTTGAGCTATATTCTTATTGGCATATTCCACCATTATTAGATCGTAGTTACATTGTTTTGATCTAATTTAATAGATTTTCGATATGTTATTTCCTAATGACAATTCAATGCAAATTAAAATAAATTATATGCTCCCTATTAATTGTTTTACACATGCATGAAGTTTGTTTTCAAAATCATCTCATTCATCAACCACACTATTCTAACTATTGATTTGGTGTGAAAGTTTGTGTCATCGCATAAAAATTTAAAGTTTGAGATTTATGGGATAAACTCATGGGTCTCTACGATATTCCAAACTTGGTCAATTTGGAATACATTTTAATTTAAATATAAAAATTGTTTATAATTTAAACACTCATATGTGTTGTTATATTTACTCATAAGTTTTTTTATATACAAATTGTATACTTAAAAACTCTTTTTAAAAAATTTGGTTTTAAGTGTTTCTCTCAAAATAACTTATTTTTCAACCTCATTTTCTAATAAATCATTTTTAATGGTCGCCAAACACTATAAATTATTTTAAAATTAAACATTTGAAAAGTTAATCCAAACATACTCTTACTCTTTCAATGTCACTTAATCGCACTATTTTTTTCTAAATTTAACGTTTATGAAACTCAAACTCTAGGCTTAGTTTTCCTAAACCCTCCCCTAGACCTAATATAGGTTTACTAGTTTTATCCTTTTAATACAACCTGAAGTGGGTAATACTCAACAAACAATACTTGTTTACACATTGCTTAGTAGGATATGATCATATAAATAATTTTGTCAATTAAAAAACATGTGAAGGTGAATAATCAAACCTCTAATCACTTAGTTAAAGATACATGCTTAAACCAATTAAGTCATCAAACTATGTTCATATCTTTTTGTTTTTTTTTTTCTTTCTTATAATATTCCTATCTACGACCTAGTTCTATTGGATTTAATAGATAAGTGAATATTATCATACAGATCCGACCCGACCCGATTTCCAAAATGTTGTTGTTGTTGTTGTTTTTATTATTATTATTATTATTATTATTATTATTTTCATACTTGGGTACTCTCGATCTCATGCTACTGATCTTGAGCCTGCCCACGTTGGGCCCATCCTTGATCTTTCTCATTAATAAAAGACGCACCCATCTCTCTCTCTCTCTCCCACTCGCTCCTCCACTCAGTCACTCTCCTTCTCATCCACCATTTTCGTGCAGAAAAACCAGAACCAGAGCAAGAGCTTTTGTCTTCTCTCTCATTTCCACTCTCGGACTCTGCTTCCCCAGAAGCAAGAGTAATGGAGCGGCTAACTCTCTCCCTCTTCTTCTTCTTCATCCTCTTCCTCTCCCCATTTGCAGGTAAATTTAAAACCTAAACCATTCTAATTTGTTTCTAATGTTAGTTTTGTTTTCGTCTTCAATGTCGTTTGTTTGTTCTGTTCTGTTTTTGCAGATTGTGGTTCAGTTGGAGTCAACTATGGCAGAATCGCCAACAATCTCCCTTCCGCCGTTAAAGTCGTCAAGCTCCTCAAATCCCACGGCCTTCAAAGGGTCAAAGTCTACGACACCGATCCGGCGGTTCTCAGAGCTCTCTCCGGCTCCGGCATTAAAGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCGAAACGCCTCACATTCGCCTACACTTGGGTCGAAAAGAATGTCGCCGCCTACTATCCTTCGACGGAAATCGAAGCCATTGCCGTCGGGAACGAGGTCTTCGTCGACCCACATAACACGACGTCGTTTCTCGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAATACAACCTCCATTCCGCCATTAAAGTCTCTTCCCCTATTGCCTTAAGCGCCTTGCAAAACTCTTACCCTGCCTCCGCCGGTTCATTCCGGCCGGAGCTCGTCGAATCGGTTTTCCGGCCAATGCTGGAGTTTCTCCGGCAAACCGGCTCCTATTTAATGGTTAACGCTTACCCATTTTTCGCTTACGAGTCTAACTCCGATGTGATTTCCTTAGACTACGCTCTCTTCCGGGATAATCCCGGCGTTGTTGATTCCGGTAGCGGTTACCGGTATTTCAGTTTGTTCGACGCCCAAATCGATGCCGTTTTTGCCGCAATGTCGGCTCTGAAATACGACGACATCAAGCTGGTGGTTACTGAAACTGGGTGGCCGTCGAAAGGCGACGAGAACGAGATCGGCGCTAGTGTTGAAAACGCCGCCGCGTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGTTGCGGCCCAAAGCAGATCTGACCGTCTATTTATTCGCCCTTTTCAACGAGAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGTTTATTTTACCCCAACGAAGAGCAGGTTTATGAAATCCCTTTTACTACAGAAGCGTTGAAGGATTACCACGACAAACCGTCACCGGCGCCGGCATCCGGCGGCGACAGGCAGTCGGCGCCGCCGGTGGGTGGCGACGGAGGTGTGTCGAAAAGTCAGACGGGGAACACGTGGTGCATTGCGAGTGGTGAAGCGGGGAAAGAGAAGCTGCAGGCGAGTCTAGATTATGCTTGTGGTGAAGGAGGAGCGGATTGCCGTCCGATCCAGCCGGGTGCCACGTGTTACAATCCCAACACGCTCGAGGCACACGCTTCGTATGCTTTCAATAGCTATTACCAGAAGAATGCACGGAAGGTCGGCACGTGTTACTTTGGAGGGGCGGCTTACGTAGTCACTCAACCACCCAGTAAGCACTCTATAATTTCTCTTTTTTTTTAATTGTTTTATTTTAAATGCGCATGTTAGTGGATCCGACCCGACCCGGCCCGTGTTTTGAGGGTAAAATGCGCTGGCGTGGTCCACGAGGTTGAAAGAACAATGAATGATGGAGCTGCACGCCAGCTCAGTAAGAAGTCAAATTTCACAGCCCACATGCCGGGCACTATTAAACAAGGGAATCTAATTTTGCTTACGTGGCATCTCCTTTTTGGGAAAACCCTACACACAAAAATATTCTTTTACAAAATGAGGGGTTGGTGGGTGTAAATTCAATTTTATCTAGGTTAAAATACAATTTGGGTAAAGTTTCAATTTTCAGTTTGGTCCCTTAATTTAGACTTATTTCCAATTTCCTTTTGAATAAAAAATTTTTAAAACTATAGGTGTCAAATTTGCAATTTAATGTTGAACAAAACTTGGCTGCATATCGGTCCTACATACCCTATTAAAATCTTGACAACTAAGAAATAGGAAGGGAGAAAATGAGAAATTTTTTTATTATTTCTCATTGTTGTCAACATTTTGATGGCGGTATGTAGGGAGATCCCTGCATATCGGTATGCAGCTAAGTTTTTTCGTATTTACTGTGGTAAAAGTTAGAAAAGGAAGAGAATGAATATTTTTTTTTGTCCCATGGGATTCAGGATAAAGGAGAAGGGGAAGCAAAAGCCATGGAATTTGAGGGAAAAGATGGATCTTTAACTCTTTTTTCTCAATGAGAAGACAAAAACTTAGAGAATTTGAGCTTTTGGGTTCTTTTTTTTAGTTATTCTAATTCCTTTGAGCAGGTATCTGAAAAGGGTTGTTTTTATTTTTCTTTTTTGGTGGCTTTTTTTTTTTGCAGAGTATGGTAGCTGTGAATTCCCAACAGGGTATTAAAGAGATGAAAAGAATCTAAAGGGAGCAGTTGAAATGAAGGGATAATAGAGAAAAAAAAAAGAGGTTACTTTTTAAGTTTGTTTTTAGAGTATGAGAGAATTCTTTTGTAGTCCAATTGCTTGGATATTGGTGGCTAAGTTAGAAGAGTTCCTTTGTCTGTAATTAACATGTTCTTCTTCCTTGTTAGACTTCATGTACTTTTGTTATCTCAAATTGATTGCTACTAATTTTAAAGCCAAAGTTCTAATAATATCTCTCCTTGATCTGAT

mRNA sequence

ATGGAGCGGCTAACTCTCTCCCTCTTCTTCTTCTTCATCCTCTTCCTCTCCCCATTTGCAGATTGTGGTTCAGTTGGAGTCAACTATGGCAGAATCGCCAACAATCTCCCTTCCGCCGTTAAAGTCGTCAAGCTCCTCAAATCCCACGGCCTTCAAAGGGTCAAAGTCTACGACACCGATCCGGCGGTTCTCAGAGCTCTCTCCGGCTCCGGCATTAAAGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCGAAACGCCTCACATTCGCCTACACTTGGGTCGAAAAGAATGTCGCCGCCTACTATCCTTCGACGGAAATCGAAGCCATTGCCGTCGGGAACGAGGTCTTCGTCGACCCACATAACACGACGTCGTTTCTCGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAATACAACCTCCATTCCGCCATTAAAGTCTCTTCCCCTATTGCCTTAAGCGCCTTGCAAAACTCTTACCCTGCCTCCGCCGGTTCATTCCGGCCGGAGCTCGTCGAATCGGTTTTCCGGCCAATGCTGGAGTTTCTCCGGCAAACCGGCTCCTATTTAATGGTTAACGCTTACCCATTTTTCGCTTACGAGTCTAACTCCGATGTGATTTCCTTAGACTACGCTCTCTTCCGGGATAATCCCGGCGTTGTTGATTCCGGTAGCGGTTACCGGTATTTCAGTTTGTTCGACGCCCAAATCGATGCCGTTTTTGCCGCAATGTCGGCTCTGAAATACGACGACATCAAGCTGGTGGTTACTGAAACTGGGTGGCCGTCGAAAGGCGACGAGAACGAGATCGGCGCTAGTGTTGAAAACGCCGCCGCGTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGTTGCGGCCCAAAGCAGATCTGACCGTCTATTTATTCGCCCTTTTCAACGAGAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGTTTATTTTACCCCAACGAAGAGCAGGTTTATGAAATCCCTTTTACTACAGAAGCGTTGAAGGATTACCACGACAAACCGTCACCGGCGCCGGCATCCGGCGGCGACAGGCAGTCGGCGCCGCCGGTGGGTGGCGACGGAGGTGTGTCGAAAAGTCAGACGGGGAACACGTGGTGCATTGCGAGTGGTGAAGCGGGGAAAGAGAAGCTGCAGGCGAGTCTAGATTATGCTTGTGGTGAAGGAGGAGCGGATTGCCGTCCGATCCAGCCGGGTGCCACGTGTTACAATCCCAACACGCTCGAGGCACACGCTTCGTATGCTTTCAATAGCTATTACCAGAAGAATGCACGGAAGGTCGGCACGTGTTACTTTGGAGGGGCGGCTTACGTAGTCACTCAACCACCCAGTAAGCACTCTATAATTTCTCTTTTTTTTTAA

Coding sequence (CDS)

ATGGAGCGGCTAACTCTCTCCCTCTTCTTCTTCTTCATCCTCTTCCTCTCCCCATTTGCAGATTGTGGTTCAGTTGGAGTCAACTATGGCAGAATCGCCAACAATCTCCCTTCCGCCGTTAAAGTCGTCAAGCTCCTCAAATCCCACGGCCTTCAAAGGGTCAAAGTCTACGACACCGATCCGGCGGTTCTCAGAGCTCTCTCCGGCTCCGGCATTAAAGTCACCGTCGATTTGCCCAACGAGCTCCTCTTCGCCGCCGCGAAACGCCTCACATTCGCCTACACTTGGGTCGAAAAGAATGTCGCCGCCTACTATCCTTCGACGGAAATCGAAGCCATTGCCGTCGGGAACGAGGTCTTCGTCGACCCACATAACACGACGTCGTTTCTCGTTCCGGCCATGAAGAACATTCACCAAGCCCTCGTCAAATACAACCTCCATTCCGCCATTAAAGTCTCTTCCCCTATTGCCTTAAGCGCCTTGCAAAACTCTTACCCTGCCTCCGCCGGTTCATTCCGGCCGGAGCTCGTCGAATCGGTTTTCCGGCCAATGCTGGAGTTTCTCCGGCAAACCGGCTCCTATTTAATGGTTAACGCTTACCCATTTTTCGCTTACGAGTCTAACTCCGATGTGATTTCCTTAGACTACGCTCTCTTCCGGGATAATCCCGGCGTTGTTGATTCCGGTAGCGGTTACCGGTATTTCAGTTTGTTCGACGCCCAAATCGATGCCGTTTTTGCCGCAATGTCGGCTCTGAAATACGACGACATCAAGCTGGTGGTTACTGAAACTGGGTGGCCGTCGAAAGGCGACGAGAACGAGATCGGCGCTAGTGTTGAAAACGCCGCCGCGTACAACGGGAATCTCGTCCGTCGGATTCTGAGCGGCGGCGGAACCCCGTTGCGGCCCAAAGCAGATCTGACCGTCTATTTATTCGCCCTTTTCAACGAGAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGTTTATTTTACCCCAACGAAGAGCAGGTTTATGAAATCCCTTTTACTACAGAAGCGTTGAAGGATTACCACGACAAACCGTCACCGGCGCCGGCATCCGGCGGCGACAGGCAGTCGGCGCCGCCGGTGGGTGGCGACGGAGGTGTGTCGAAAAGTCAGACGGGGAACACGTGGTGCATTGCGAGTGGTGAAGCGGGGAAAGAGAAGCTGCAGGCGAGTCTAGATTATGCTTGTGGTGAAGGAGGAGCGGATTGCCGTCCGATCCAGCCGGGTGCCACGTGTTACAATCCCAACACGCTCGAGGCACACGCTTCGTATGCTTTCAATAGCTATTACCAGAAGAATGCACGGAAGGTCGGCACGTGTTACTTTGGAGGGGCGGCTTACGTAGTCACTCAACCACCCAGTAAGCACTCTATAATTTCTCTTTTTTTTTAA

Protein sequence

MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAPASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPSKHSIISLFF
Homology
BLAST of Spg007785 vs. NCBI nr
Match: XP_038886813.1 (LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida])

HSP 1 Score: 867.1 bits (2239), Expect = 7.1e-248
Identity = 433/469 (92.32%), Postives = 449/469 (95.74%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60
           MER TL  FF F LFLS FA+ GS+GVNYGRIANNLPSAVKVVKLLK+HGLQRVKVYDTD
Sbjct: 1   MERPTLCFFFIFPLFLSSFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 180
           VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYP+SAGSFRPELVE+V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVETV 180

Query: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPMLEFLRQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNADVISLDYALFRDNPGVVDAGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASVENAAAYNGNLVRRIL+GGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILNGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKKNGP SERNYGLFYPNEE+VYEIPFTTE LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPASERNYGLFYPNEEKVYEIPFTTEGLKDYHDKPSPAP 360

Query: 361 ASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGG-----ADCR 420
            +GG+  S PP  GDGGVSKSQTGNTWC+ASGEAGKEKLQ+SLDYACGEGG     ADCR
Sbjct: 361 VTGGNGPSGPPESGDGGVSKSQTGNTWCVASGEAGKEKLQSSLDYACGEGGXXXXXADCR 420

Query: 421 PIQPGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           PIQPGATCYNPNTLEAHASYAFNSYYQKN+RKVGTCYFGGAAYVVTQPP
Sbjct: 421 PIQPGATCYNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 469

BLAST of Spg007785 vs. NCBI nr
Match: KAG6572415.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012014.1 Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 867.1 bits (2239), Expect = 7.1e-248
Identity = 434/464 (93.53%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60
           MERLTLS FF FILFLSPFA+ GS+GVNYGRIANNLPSAVKVVKLLK+HGLQRVKVYDTD
Sbjct: 1   MERLTLS-FFIFILFLSPFAESGSIGVNYGRIANNLPSAVKVVKLLKTHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYP+SAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASV NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 ASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPG 420
           A+GGD Q+     GDGGVSKSQTGNTWC+ASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGDGQTGSG-SGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKNARK+GTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

BLAST of Spg007785 vs. NCBI nr
Match: XP_022952844.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata])

HSP 1 Score: 865.1 bits (2234), Expect = 2.7e-247
Identity = 434/464 (93.53%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60
           MERLTLS FF F+LFLSPFA+ GS+GVNYGRIAN+LPSAVKVVKLLKSHGLQRVKVYDTD
Sbjct: 1   MERLTLS-FFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAYTWVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYP+SAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASV NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 ASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPG 420
           A+GGD Q A    GDGGVSKSQTGNTWC+ASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGDGQ-AGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKNARK+GTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

BLAST of Spg007785 vs. NCBI nr
Match: XP_004140121.1 (glucan endo-1,3-beta-glucosidase 12 [Cucumis sativus] >KGN47963.1 hypothetical protein Csa_003377 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 5.1e-246
Identity = 427/465 (91.83%), Postives = 449/465 (96.56%), Query Frame = 0

Query: 1   MERL-TLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDT 60
           ME+L +L  F+FF+LFLSPFA+ GS+GVNYGRI N+LPSAVKVVKLLKSHGLQRVKVYDT
Sbjct: 1   MEQLKSLWFFYFFLLFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120
           DPAVL+ALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLKALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVES 180
           FVDPHNTTSFLVPAMKNIHQALVKYNLHS IKVSSPIALSALQNSYP+SAGSFRPELVE+
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVET 180

Query: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFD 240
           VFRPMLEFLRQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 300
           AQIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPA 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKD+ DKPSP 
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPK 360

Query: 361 PASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQP 420
           P SGG+  +APP  GDGGVSKSQTGNTWC+ASGEAGKEKLQ+ LDYACGEGGADCRPIQ 
Sbjct: 361 PVSGGNAPTAPPASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQV 420

Query: 421 GATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           GATCYNPNTLEAHASYAFNSYYQKN+RKVGTCYFGGAAYVVTQPP
Sbjct: 421 GATCYNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 465

BLAST of Spg007785 vs. NCBI nr
Match: XP_023553980.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860.1 bits (2221), Expect = 8.7e-246
Identity = 431/464 (92.89%), Postives = 448/464 (96.55%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60
           MERLTLS FF F+LFL PFA+ GS+GVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD
Sbjct: 1   MERLTLS-FFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FA TWVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFANTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYP+SAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASV NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 ASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPG 420
           A+GGD Q+     GDGGVSKSQTGNTWC+ASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGDGQTGSG-SGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKNARK+GTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

BLAST of Spg007785 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.6e-99
Identity = 195/474 (41.14%), Postives = 288/474 (60.76%), Query Frame = 0

Query: 2   ERLTLSLFFFF-ILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 61
           +RL L  + F  IL    F     +G+ YGR A+NLPS  +V +L++   ++ V++YD +
Sbjct: 3   QRLNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDAN 62

Query: 62  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 121
             VL+A + +GI++ + +PN  L A A+  +   TW+  N+  YYPST+I +I+VG EV 
Sbjct: 63  IDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVT 122

Query: 122 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 181
             P N T  ++PAM+NIH AL K  L   IK+SS  +L+ L  S+P S+ SF  +   + 
Sbjct: 123 EAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAF 182

Query: 182 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 241
            +PMLEFL +  S  M++ YP++AY  +++ + L+YALF  +  VVD  +G  Y ++FDA
Sbjct: 183 LKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDA 242

Query: 242 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 301
           Q+DA++ A++A+ +  +K++VTE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP
Sbjct: 243 QLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTP 302

Query: 302 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFT---------TEALKD 361
            +P  ++ VYLF+LFNEN+K G  SERN+G+FY N   VY + FT         T +   
Sbjct: 303 AKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTG 362

Query: 362 YHDKPSPAPASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGG 421
               PS +P   G+       GG GG  K      WCIAS +A   +LQ +LD+ACG G 
Sbjct: 363 TSPSPSSSPIINGNSTVTIGGGGGGGTKK------WCIASSQASVTELQTALDWACGPGN 422

Query: 422 ADCRPIQPGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPS 466
            DC  +QP   C+ P+T+ +HASYAFN+YYQ++      C F GA+  V + PS
Sbjct: 423 VDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPS 469

BLAST of Spg007785 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.4e-99
Identity = 193/460 (41.96%), Postives = 283/460 (61.52%), Query Frame = 0

Query: 11  FFILFLSPFADC---GSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRAL 70
           F I  L    DC   G VGV YGR A++LP+  KVV+L++ H ++ V++YD +  VL+A 
Sbjct: 9   FSISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAF 68

Query: 71  SGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPH-NT 130
             + I++ + +PN  L A ++  +   TW++ +V  YYP+T+I  I VG E   DPH N 
Sbjct: 69  GNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINA 128

Query: 131 TSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESVFRPMLE 190
           +SF+VPAM+N+  AL K  L   IKVS+ ++L  L  S+P SAG+F         RPMLE
Sbjct: 129 SSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPMLE 188

Query: 191 FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVF 250
           FL +  S  M++ YP++AY  + + +SLDY LF  +  V+D  +G  Y ++FDAQ+DA++
Sbjct: 189 FLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALY 248

Query: 251 AAMSALKYDDIKLVVTETGWPSKGDENE-IGASVENAAAYNGNLVRRILSGGGTPLRPKA 310
            A++AL +  IK++VTETGWP+KG   E   AS +NA  YN N++R +++  GTP +P  
Sbjct: 249 YALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGE 308

Query: 311 DLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAPASGGD 370
            + VY+F+LFNEN+K G  SERN+GLFYP++  VY++ FT ++   +H   S        
Sbjct: 309 AMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKS-NGFHSNSS-------- 368

Query: 371 RQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPGATCYN 430
                      G + S + N+WCIAS +A +  L+ +LD+ACG G  DC  IQP   C+ 
Sbjct: 369 -----------GTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQ 428

Query: 431 PNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPS 466
           P+TL +HAS+ FNSY+Q+N      C FGGA   V + PS
Sbjct: 429 PDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPS 447

BLAST of Spg007785 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 339.3 bits (869), Expect = 6.8e-92
Identity = 188/469 (40.09%), Postives = 275/469 (58.64%), Query Frame = 0

Query: 6   LSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLR 65
           L L F F+   S  +    +GVN G    N+PS  +VV LLKS  + RV++YD D ++L 
Sbjct: 4   LLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLL 63

Query: 66  ALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHN 125
           A + +G++V + +PN+ L   ++    A  WV +NVAAYYP+T I  IAVG+EV     N
Sbjct: 64  AFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTN 123

Query: 126 TTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESVFRPML 185
             S LV A+K I  ALV  NL   IKVS+P + + + +S+P S   F  +  + V  P+L
Sbjct: 124 AASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN-KTWDPVIVPLL 183

Query: 186 EFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRD---NPGVVDSGSGYRYFSLFDAQI 245
           +FL+ TGS L++N YP+F Y  ++ VI LDYALF+    N   VD+ +   Y ++FDA +
Sbjct: 184 KFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIV 243

Query: 246 DAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLR 305
           DA + AMS L + +I +VVTE+GWPSKG  +E  A+VENA  YN NL++ +++  GTP  
Sbjct: 244 DAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKH 303

Query: 306 PKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAPAS 365
           P   +T Y++ L+NE+ + GP SE+N+GLFY N   VY +                    
Sbjct: 304 PGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTL-------------------- 363

Query: 366 GGDRQSAPPVGGDGGVSKSQTGN-TWCIASGEAGKEKLQASLDYACGEGGADCRPIQPGA 425
                    + G G +  + T N T+CIA  +  ++ LQA+LD+ACG G  DC  +  G 
Sbjct: 364 --------RLAGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGE 423

Query: 426 TCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPSKHSII 471
           +CY P+ + AH++YAFN+YYQK  +  G+C F G A V T  PS+ + +
Sbjct: 424 SCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCV 443

BLAST of Spg007785 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 3.4e-91
Identity = 187/472 (39.62%), Postives = 287/472 (60.81%), Query Frame = 0

Query: 4   LTLSLFFFFILFLSPFADCGS---VGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 63
           L++S+ +F ++FLS F    +   +GVNYG++A+NLP   + VKLL+S  +Q+V++Y  D
Sbjct: 3   LSISI-YFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGAD 62

Query: 64  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 123
           PA+++AL+G+G+ + +   N  + + A     A  W+  NV  +YP+++I  I VGNE+ 
Sbjct: 63  PAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEIL 122

Query: 124 V--DPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVE 183
           +  DP N  + L+PAM+N+ +AL   +L   IKVS+  +++ L +S P S+GSF     +
Sbjct: 123 MSNDP-NLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAG-YQ 182

Query: 184 SVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLF 243
           +  + +L+FL  TGS   +N YPFFAY+S+    +L + LF  N G VDS +G +Y ++F
Sbjct: 183 TGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMF 242

Query: 244 DAQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGG 303
           DAQ+DAV +A+ ++ ++ +++VV ETGW S+GD NE+GASV+NA AYNGNL+  + S  G
Sbjct: 243 DAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVG 302

Query: 304 TPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSP 363
           TPL P   +  Y+FAL++EN K GP+SER +GLF  +   VY++                
Sbjct: 303 TPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGL-------------- 362

Query: 364 APASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQ 423
             A        P        S   T + WC+    A  E+LQASLD+ACG  G DC  IQ
Sbjct: 363 --AKSSSSSQTP--------SGKVTSSGWCVPKKGATNEELQASLDWACGH-GIDCGAIQ 422

Query: 424 PGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPSKHSII 471
           PG  C+ PN + +HA+YA N Y+QK+ ++   C F   A V +Q PS ++ +
Sbjct: 423 PGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCV 446

BLAST of Spg007785 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 332.8 bits (852), Expect = 6.4e-90
Identity = 189/467 (40.47%), Postives = 273/467 (58.46%), Query Frame = 0

Query: 4   LTLSLFFFFILFLSPFADCGS-VGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPA 63
           L+LSL        SP AD GS +GVN G   +++P   +VV LLK+  ++ +++Y+ DP 
Sbjct: 10  LSLSLLVLASASPSPPADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPG 69

Query: 64  VLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVD 123
           +L AL+ +GIKV + +PN+ L    +  + A  WV++NV A+YP+T I A++VG+EV   
Sbjct: 70  LLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTS 129

Query: 124 PHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESVFR 183
             N    LV A+KN+H AL+  NL   IKVS+P++ S + + +P S   F   L  +V  
Sbjct: 130 LSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSL-NAVIV 189

Query: 184 PMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR---DNPGVVDSGSGYRYFSLFD 243
           P+L FL+ T SYLMVN YP+  Y  ++ VI LDYALF+    N   VD+ +  RY + FD
Sbjct: 190 PLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFD 249

Query: 244 AQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 303
           A +DA + AM+ L + +I ++VTE+GWPSKG+ NE  A+++NA  YN NL+R +L+  GT
Sbjct: 250 AMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGT 309

Query: 304 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPA 363
           P RP   ++ Y++ L+NE+ K G  SE+N+GLF  N E VY +  T              
Sbjct: 310 PKRPGIAVSTYIYELYNEDTKAG-LSEKNWGLFNANGEPVYVLRLT-------------- 369

Query: 364 PASGGDRQSAPPVGGDGGVSKSQTGN-TWCIASGEAGKEKLQASLDYACGEGGADCRPIQ 423
                           G V  + T N T+C A   A  + LQA+LD+ACG G  DC PI+
Sbjct: 370 --------------NSGSVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIK 429

Query: 424 PGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPS 466
            G TCY P+ + AHA+YAF++YY +       C F G A + T  PS
Sbjct: 430 QGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

BLAST of Spg007785 vs. ExPASy TrEMBL
Match: A0A6J1GMW6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 434/464 (93.53%), Postives = 450/464 (96.98%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60
           MERLTLS FF F+LFLSPFA+ GS+GVNYGRIAN+LPSAVKVVKLLKSHGLQRVKVYDTD
Sbjct: 1   MERLTLS-FFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAYTWVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYP+SAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASV NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 ASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPG 420
           A+GGD Q A    GDGGVSKSQTGNTWC+ASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGDGQ-AGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKNARK+GTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPP 462

BLAST of Spg007785 vs. ExPASy TrEMBL
Match: A0A0A0KEL0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 427/465 (91.83%), Postives = 449/465 (96.56%), Query Frame = 0

Query: 1   MERL-TLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDT 60
           ME+L +L  F+FF+LFLSPFA+ GS+GVNYGRI N+LPSAVKVVKLLKSHGLQRVKVYDT
Sbjct: 1   MEQLKSLWFFYFFLLFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDT 60

Query: 61  DPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120
           DPAVL+ALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV
Sbjct: 61  DPAVLKALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEV 120

Query: 121 FVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVES 180
           FVDPHNTTSFLVPAMKNIHQALVKYNLHS IKVSSPIALSALQNSYP+SAGSFRPELVE+
Sbjct: 121 FVDPHNTTSFLVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVET 180

Query: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFD 240
           VFRPMLEFLRQTGSYLMVNAYPFFAYESN+DVISLDYALFRDNPGVVD+GSGYRYF+LFD
Sbjct: 181 VFRPMLEFLRQTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFD 240

Query: 241 AQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 300
           AQIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT
Sbjct: 241 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 300

Query: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPA 360
           PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKD+ DKPSP 
Sbjct: 301 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPK 360

Query: 361 PASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQP 420
           P SGG+  +APP  GDGGVSKSQTGNTWC+ASGEAGKEKLQ+ LDYACGEGGADCRPIQ 
Sbjct: 361 PVSGGNAPTAPPASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQV 420

Query: 421 GATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           GATCYNPNTLEAHASYAFNSYYQKN+RKVGTCYFGGAAYVVTQPP
Sbjct: 421 GATCYNPNTLEAHASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPP 465

BLAST of Spg007785 vs. ExPASy TrEMBL
Match: A0A6J1I0V3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.5e-246
Identity = 430/464 (92.67%), Postives = 447/464 (96.34%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60
           MERLTLS FF F+LFL PFA+ GS+GVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD
Sbjct: 1   MERLTLS-FFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 60

Query: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120
           PAVLRALSGSGIKVTVDLPNELLFAAAKRL+FAYTWVEKNVAAYYPSTEIEAIAVGNEVF
Sbjct: 61  PAVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 120

Query: 121 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 180
           VDPHNTTSFLVPAMKNIHQALVK+NLHSAIKVSSPIALSALQNSYP+SAGSFRPELVE V
Sbjct: 121 VDPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPV 180

Query: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 240
           FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVDSGSGYRYF+LFDA
Sbjct: 181 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDA 240

Query: 241 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 300
           QIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGAS  NAAAYNGNL+RRILSGGGTP
Sbjct: 241 QIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGTP 300

Query: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAP 360
           LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE+VY+IPFTTE LKDYHDKPSPAP
Sbjct: 301 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDKPSPAP 360

Query: 361 ASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPG 420
           A+GGD Q+     GDGGVSKSQTGNTWC+ASGEAGKEKLQA LDYACGEGGADCRPIQP 
Sbjct: 361 ATGGDGQTGSG-HGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           ATCYNPNTL+AHASYAFNSYYQKN RK+GTCYFGGAAYVVTQPP
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPP 462

BLAST of Spg007785 vs. ExPASy TrEMBL
Match: A0A6J1ERV0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.7e-242
Identity = 425/470 (90.43%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSP----FADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKV 60
           MERLTLS FF F+L L+       + GS+GVNYGRIANNLPSA+KVV LLKSHGLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLAASFPVLTEAGSIGVNYGRIANNLPSALKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVG 120
           YDTDPAVLRA+SGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKN+ AYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPEL 180
           NEVFVDPHNTTSFL+PAMKNIHQALVKYNLHS+IKVSSPIALSALQNSYP+SAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 VESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFS 240
           +ESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVD+GSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDK- 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE VY+IPFTTE LKDYHDK 
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 -PSPAPASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADC 420
            PSP+PA+GGD +S  PV GDG VSKSQTGNTWC+ASGEAGKEKLQASLDYACGEGGADC
Sbjct: 361 SPSPSPATGGDGKSG-PVNGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC 420

Query: 421 RPIQPGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           RPIQ GATCYNPNTLEAHASYAFNSYYQKN R  GTCYFGGAAYVVTQPP
Sbjct: 421 RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRMTGTCYFGGAAYVVTQPP 469

BLAST of Spg007785 vs. ExPASy TrEMBL
Match: A0A6J1JLE7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.2e-242
Identity = 425/470 (90.43%), Postives = 443/470 (94.26%), Query Frame = 0

Query: 1   MERLTLSLFFFFILFLSP----FADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKV 60
           MERLTLS FF F+L LSP      + GS+GVNYGRIANNLPSAVKVV LLKSHGLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLSPSFAVVTEAGSIGVNYGRIANNLPSAVKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVG 120
           YDTDPAVL A+SGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKN+ AYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLTAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPEL 180
           NEVFVDPHNTTSFL+PAMKNIHQALVKYNLHS+IKVSSPIALSALQNSYP+SAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 VESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFS 240
           +ESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR+NPGVVD+GSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASV NAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVGNAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDK- 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE VY+IPFTTE LKDYHDK 
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 -PSPAPASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADC 420
            PSP+PA+GGD +S  PV GDG VSKSQ+GNTWC+ASGEAGKEKLQASLDYACGEGGADC
Sbjct: 361 SPSPSPATGGDGKSG-PVNGDGNVSKSQSGNTWCVASGEAGKEKLQASLDYACGEGGADC 420

Query: 421 RPIQPGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           RPIQ GATCYNPNTLEAHASYAFNSYYQKN RK GTCYFGGAAYVVTQ P
Sbjct: 421 RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQQP 469

BLAST of Spg007785 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 705.7 bits (1820), Expect = 2.6e-203
Identity = 342/461 (74.19%), Postives = 409/461 (88.72%), Query Frame = 0

Query: 6   LSLFFFFILFLSPF--ADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAV 65
           L+   F +L LSPF  +D GS+GVNYGRI++ LPSA KVV+LLKS G+ RVK++D DP+V
Sbjct: 4   LTPLIFLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSV 63

Query: 66  LRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDP 125
           L+ALSGSGIKVTVDLPNELLF+AAKR +FA +WV++NVAAY+PST+IE+IAVGNEVFVD 
Sbjct: 64  LKALSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDT 123

Query: 126 HNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESVFRP 185
           HNTTSFL+PAM+NIH+AL+ +NLHS IK+SSP+ALSALQNSYP+S+GSFRPEL++SV +P
Sbjct: 124 HNTTSFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKP 183

Query: 186 MLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQID 245
           ML+FLR+TGS LM+N YPFFAYE NSDVI LDYAL R+NPG+VDSG+G RYF+LFDAQID
Sbjct: 184 MLDFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQID 243

Query: 246 AVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRP 305
           AVFAAMSALKYDDI+++VTETGWPSKGDENE+GA++ NAA+YNGNL+RRIL+ GGTPLRP
Sbjct: 244 AVFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRP 303

Query: 306 KADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAPASG 365
           KADLTVYLFALFNENKK GPTSERNYGLF+P+E++VY+IPFTTE LK Y D     P +G
Sbjct: 304 KADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRD-GGHTPVTG 363

Query: 366 GDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPGATC 425
           GD+ + PP+   GGVSKS  G TWC+A+G+AG+E+LQ  LDYACGEGGADCRPIQPGA C
Sbjct: 364 GDQVTKPPM--SGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANC 423

Query: 426 YNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           Y+P+TLEAHAS+AFNSYYQK  R  G+CYFGGAAYVV+QPP
Sbjct: 424 YSPDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPP 461

BLAST of Spg007785 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 596.7 bits (1537), Expect = 1.7e-170
Identity = 300/468 (64.10%), Postives = 361/468 (77.14%), Query Frame = 0

Query: 6   LSLFFFFILFLSPF-------ADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYD 65
           +++F   +L LS F       AD G +GVNYGRIA+NLP+  KVV+LLK+ G+ R+K+YD
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 66  TDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNE 125
           T+  VL AL+ SGIKV V LPNE L +AA   ++  TWV+ N+  Y P+T+IEAIAVGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 126 VFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVE 185
           VFVDP NTT++LVPAMKN+  +LVK+NL  +IK+SSPIALSAL +SYP SAGSF+PEL+E
Sbjct: 121 VFVDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIE 180

Query: 186 SVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLF 245
            V +PML+ LR+T S+LMVNAYPFFAY +N+D ISLDYALF++N G VDSG+G +Y SL 
Sbjct: 181 PVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLL 240

Query: 246 DAQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGG 305
           DAQIDAVFAAMSA+ ++D+KLVVTETGWPS GDENEIGA   NAAAYNG LV+R+L+G G
Sbjct: 241 DAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNG 300

Query: 306 TPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSP 365
           TPL+PK  L VYLFALFNEN+K GPTSERNYGLFYPNE +VY++    ++     +K   
Sbjct: 301 TPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKV 360

Query: 366 APASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQ 425
            P                 V  S  G TWC+A+G+  KEKLQ  LDYACGEGGADCRPIQ
Sbjct: 361 VP-----------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQ 420

Query: 426 PGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPSK 467
           PGATCYNP +LEAHASYAFNSYYQKNAR VGTC FGGAAYVV+QPPSK
Sbjct: 421 PGATCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPSK 451

BLAST of Spg007785 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 592.8 bits (1527), Expect = 2.4e-169
Identity = 298/466 (63.95%), Postives = 359/466 (77.04%), Query Frame = 0

Query: 6   LSLFFFFILFLSPF-------ADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYD 65
           +++F   +L LS F       AD G +GVNYGRIA+NLP+  KVV+LLK+ G+ R+K+YD
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 66  TDPAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNE 125
           T+  VL AL+ SGIKV V LPNE L +AA   ++  TWV+ N+  Y P+T+IEAIAVGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 126 VFVDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVE 185
           VFVDP NTT++LVPAMKN+  +LVK+NL  +IK+SSPIALSAL +SYP SAGSF+PEL+E
Sbjct: 121 VFVDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIE 180

Query: 186 SVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLF 245
            V +PML+ LR+T S+LMVNAYPFFAY +N+D ISLDYALF++N G VDSG+G +Y SL 
Sbjct: 181 PVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLL 240

Query: 246 DAQIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGG 305
           DAQIDAVFAAMSA+ ++D+KLVVTETGWPS GDENEIGA   NAAAYNG LV+R+L+G G
Sbjct: 241 DAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNG 300

Query: 306 TPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSP 365
           TPL+PK  L VYLFALFNEN+K GPTSERNYGLFYPNE +VY++    ++     +K   
Sbjct: 301 TPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNKEKV 360

Query: 366 APASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQ 425
            P                 V  S  G TWC+A+G+  KEKLQ  LDYACGEGGADCRPIQ
Sbjct: 361 VP-----------------VKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQ 420

Query: 426 PGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
           PGATCYNP +LEAHASYAFNSYYQKNAR VGTC FGGAAYVV+QPP
Sbjct: 421 PGATCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPP 449

BLAST of Spg007785 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 585.9 bits (1509), Expect = 3.0e-167
Identity = 299/458 (65.28%), Postives = 357/458 (77.95%), Query Frame = 0

Query: 9   FFFFILFLSPF-ADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDPAVLRAL 68
           +F  + FLS   A  G VGVNYGRIANNLPS  KVV LLKS G+ R+K++DTD  VL AL
Sbjct: 8   YFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLTAL 67

Query: 69  SGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTT 128
           + S IKV V LPNELL +AA   +FA  W++ ++  Y+P+TEIEAIAVGNEVFVDP   T
Sbjct: 68  ANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-TIT 127

Query: 129 SFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESVFRPMLEF 188
            +LV AMKNIH +LVKY L  AIK+SSPIALSAL NSYP S+GSF+PEL+E V +PML  
Sbjct: 128 PYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPMLAL 187

Query: 189 LRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDAQIDAVFA 248
           L+QT SYLMVNAYPFFAY +N+D ISLDYALF++N G +DSG+G +Y SLFDAQIDAV+A
Sbjct: 188 LQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAVYA 247

Query: 249 AMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADL 308
           A+SA+ +  +K++VTETGWPS GDENEIGAS  NAAAYN  LV+R+L+G GTPLRP   L
Sbjct: 248 ALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTEPL 307

Query: 309 TVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFTTEALKDYHDKPSPAPASGGDRQ 368
            VYLFALFNEN+K GPTSERNYGLFYPNE +VY +PFT                    ++
Sbjct: 308 NVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFT--------------------KK 367

Query: 369 SAPPVGGD-GGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGGADCRPIQPGATCYNP 428
           S  PV G+ G V  +  G+TWC+++GE  KEKLQ +LDYACGEGGADCRPIQPGATCY+P
Sbjct: 368 STTPVNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHP 427

Query: 429 NTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPP 465
            +LEAHASYAFNSYYQKN+R+VGTC+FGGAA+VVTQPP
Sbjct: 428 ESLEAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPP 444

BLAST of Spg007785 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 367.1 bits (941), Expect = 2.2e-101
Identity = 196/476 (41.18%), Postives = 290/476 (60.92%), Query Frame = 0

Query: 2   ERLTLSLFFFF-ILFLSPFADCGSVGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTD 61
           +RL L  + F  IL    F     +G+ YGR A+NLPS  +V +L++   ++ V++YD +
Sbjct: 3   QRLNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDAN 62

Query: 62  PAVLRALSGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVF 121
             VL+A + +GI++ + +PN  L A A+  +   TW+  N+  YYPST+I +I+VG EV 
Sbjct: 63  IDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVT 122

Query: 122 VDPHNTTSFLVPAMKNIHQALVKYNLHSAIKVSSPIALSALQNSYPASAGSFRPELVESV 181
             P N T  ++PAM+NIH AL K  L   IK+SS  +L+ L  S+P S+ SF  +   + 
Sbjct: 123 EAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAF 182

Query: 182 FRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRDNPGVVDSGSGYRYFSLFDA 241
            +PMLEFL +  S  M++ YP++AY  +++ + L+YALF  +  VVD  +G  Y ++FDA
Sbjct: 183 LKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDA 242

Query: 242 QIDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTP 301
           Q+DA++ A++A+ +  +K++VTE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP
Sbjct: 243 QLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTP 302

Query: 302 LRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEEQVYEIPFT---------TEALKD 361
            +P  ++ VYLF+LFNEN+K G  SERN+G+FY N   VY + FT         T +   
Sbjct: 303 AKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTG 362

Query: 362 YHDKPSPAPASGGDRQSAPPVGGDGGVSKSQTGNTWCIASGEAGKEKLQASLDYACGEGG 421
               PS +P   G+       GG GG  K      WCIAS +A   +LQ +LD+ACG G 
Sbjct: 363 TSPSPSSSPIINGNSTVTIGGGGGGGTKK------WCIASSQASVTELQTALDWACGPGN 422

Query: 422 ADCRPIQPGATCYNPNTLEAHASYAFNSYYQKNARKVGTCYFGGAAYVVTQPPSKH 468
            DC  +QP   C+ P+T+ +HASYAFN+YYQ++      C F GA+  V + PSK+
Sbjct: 423 VDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSKY 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886813.17.1e-24892.32LOW QUALITY PROTEIN: glucan endo-1,3-beta-glucosidase 12 [Benincasa hispida][more]
KAG6572415.17.1e-24893.53Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022952844.12.7e-24793.53glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata][more]
XP_004140121.15.1e-24691.83glucan endo-1,3-beta-glucosidase 12 [Cucumis sativus] >KGN47963.1 hypothetical p... [more]
XP_023553980.18.7e-24692.89glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8VYE52.6e-9941.14Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU93.4e-9941.96Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q9ZU916.8e-9240.09Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Q9M0693.4e-9139.62Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q9C7U56.4e-9040.47Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GMW61.3e-24793.53(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 P... [more]
A0A0A0KEL02.5e-24691.83(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3... [more]
A0A6J1I0V35.5e-24692.67(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=... [more]
A0A6J1ERV01.7e-24290.43(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 P... [more]
A0A6J1JLE78.2e-24290.43(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=... [more]
Match NameE-valueIdentityDescription
AT2G05790.12.6e-20374.19O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.21.7e-17064.10O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.12.4e-16963.95O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.13.0e-16765.28O-Glycosyl hydrolases family 17 protein [more]
AT4G29360.22.2e-10141.18O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 386..470
e-value: 4.3E-44
score: 162.5
IPR012946X8 domainPFAMPF07983X8coord: 386..457
e-value: 4.5E-22
score: 78.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..353
e-value: 7.5E-127
score: 424.7
NoneNo IPR availableGENE3D1.20.58.1040coord: 381..471
e-value: 1.7E-10
score: 43.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 350..380
NoneNo IPR availablePANTHERPTHR32227:SF350O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN-RELATEDcoord: 11..468
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 25..344
e-value: 2.5E-91
score: 306.6
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 257..270
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 11..468
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 25..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg007785.1Spg007785.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds