Spg007678 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg007678
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (Adenine(37)-N6)-methyltransferase
Location	scaffold2: 1832359 .. 1836145 (+)
RNA-Seq Expression	Spg007678
Synteny	Spg007678

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGAAGATCCCAAACCTAGAGAAGAAATAACAAAGCGGGAAACTGGTTATCAAACCTCGGGCTGCCGGATCGAGTTCGCCTGAGCGCTGAAGCTGCTTTCCAATTCCAACTCTGTAAGACCAAATGGCCCACGGCGCAGAATCAAGGTGGCTGCCGCCCGCCATGAGCGTTACACTGGCGTTTATCTCCGCCTCTGCCTCCGCCATCATATCTAGTAAAACTTTGAAACTTTTAAAGATCCTTGAAGTCCTCTCTCTCAATTTCTCCACTTTCCTACATTTTTCCTCACAACTGGAGTTTTCTGCTTTTTCCCAGTTTATCTAGCGACGAGGAAAACCAGAGAGTTGGATTCCAAGGTTCAAGAGCTCGAGACTTCTCTGGCAGCTTCTTTAAAGAAAACCGCTGCCGAGAGAAAAGGCCGAATACGGGCCCAGCAGGTATTTCATTATATGCTCCTCTGCATCGCTTCACTTTCCTATGTTATTTTTGATATTTTGATTTCTTTTTTCTCCTAATTCGTAGGCTTTAAGGAAAGCACTTGCAGAACCAAAGTCTGATAGCGTGGAATGCACTTCGTATCCCATGACACCAATTGGTGTCATCCAGTCATCCTTCTCTACTAGGTTAGTTTAAGATTGCATTATCCGGCAGTCTAAACATCAATTGTAGGGTTGTTGTTTAGTTTTCTCATTGTTCTGTTCATACAACCGAGTAAACAAATGGATATGTTTAGGCCTTAATTCTTCAATTAGCTGTAGAAATCGAGCAATCAACAACTTTTGTTGGGTTCTTTTAGGAATGGGACTCCAAGGCAATCTTTAATTGTACCTCTTGCGAGGGCACGGTTGGTGTTCTCTGCTCGTATTCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCACTGCTGGATTATATATGTCTTTCATTTGAATACAGATCTTGAGAAGCTGTGGAAGGATCCGTCTAAATCAAAATTCAAGGCAAAAGTACGTTTCTTCATTTGTTGATGCTCCTTAAAAATGGAATTCATAGTTTTATTATTCTTATAGTTTCCCAAAATCCCATAAAAGGACAGCTCAATGGTAGGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCAATCGGACTCACTATTGCGAAGGTAAGATAAGAATTTCAATCTTGTGACTTTTGAGCACATGGTCCAATATGTTTTGTATGGTTAGGCTGTTTCAATTTCTTTTAGTATTAGAAATTCATGGTTACAAGCTTACAGTTGTAAGTGTTAGACGACATCACGACATTCTAATGCAATTAATAAATGGGGGATTATTAAATTAAATCTAAGATTAGGAATCTGCTATGTTACCACTTCAGGTGGAGGCTGTCCAGGGACATCAAATTATACTCTCTGGTGCCGATCTTGTTGATGGAACTGTAAGTGTTTTTGTTTCTAATTTTTTCTAATCAGCAGGATAAAAGATTTAATCTTGAAATAAATCATTACTTCTTGTGGTGATGATTGTTATGATTTATTGCTTTGTTGTTGTTACAGCCAGTTCTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAGGATGCAAAAGTCCCAGAGTGGTTAATGGTATTTCTTAAATATTTCTATGACTAAATGTTATCTTAGAATTTCTTTGATGCTTATGACTAAATGTATGTGGTATTTGTATAGATGAAAAAAAAAATCAATTTTTATGTGGTAATTTTAGCCACTCATTCTTTCATTCATTTGTCCCAACGCAGATTTATTTTGCTTCCAAATTTCAAAATGTGGAAAATCTCTACACATATGCATGCCATATAATTAGTTGGTGCAAATAACTTAAGAAATTTTAGCTTCCCAGATACTATTTGGTGCTATTTCTCATAGGTGGAGTTTTTGATACTCTATGACCATGTAATATGTTTCATACGTTCTTCATGGCAATCTGACATCATATTTTACGTGAATGCAGGGGGAAAATTTGTTGAGTGTTGCATCCATCTGTTTCTCTGAAGAATTTACTTCCACTCTATCGGAGTGTTGGCCACTAATGGTGAGTTTTTTTTTACCCTTTAATTTTTTATTAAAGTTGTTCTGGGTAGTTCATTTTTGATACTGATTAGAAGTTTAGCTTGTTCATGCATTATTTCACTATTTGTTTTGCACTGAAGAAAGTAATAAACAGTAAACAACAGTATTTTACCTAGCCATTCTAAGTTTCTTTAAAATTTGGATATTGGAGAGGTCTAATGTAAAATCAATAGTCTGTTTTTTTTTCATTGGTATTAATCAAAGGGAACCTTTCAAACTATTATATTTTTTTCCCCAAGGGTCCTTACTGGTTAGGGCTTGGAGTTTTGGGTGTTAATCAGCCTTAGGTTGGAACCTCAAATAGAAAGCTTAGTAAAACACAAGTGAAAACTCTTGTGGTCTCAGGCTGGTGTTGTAGCACGCAAAGTCTAAATGCCTAATGAAAATTATGCTTCTGGAATTTTACATTATGGACAGTGATCTGGGTCATTTCTTATTCTTTTACTCCTTTATAGTCGACCTTGACTTCAAAAAAAGAAAGAGAGAAAGAGAGAGCTGCTTTGTAGTCTGATTACCATTTTCATGAAACTCCATATTACAAAGAGCCAGTGCTCTCTACTTTCATGTTGTAAAAAGTAGGATATTCTTGGTCGAGTATGTCTGATCCCATCTTTGCAACAAAAATTACACGTATCATACTTCTCTTGTTTCAGGAAAAGAAGTCGCTTTATTCATCCGCAGATGAGTTGAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCTATGTCACAACGTAGTCGGCCTCATGACTCCCTCGTTAAGACAACAACAGGACATGAACTGTCCGATGGTAGTTCAGATTCAGATGGTCACCTAGATGACAAAGTTTCGAGTGGTACCCAAAGTTCTTCTGGGAGCATTATCTATCACCTACTTGTAGATGGATTAGATGTGTCGTACACGATTGGTTGCCATGGTGACGTGGTTGTTGGCAAAGTAGTACTCTCAGCACCAAGCTCAAGAACAAGCAGCGATCAACACAAAGAAAACTCTTCTGAATAGGCAAATCAAGGTAACTTGTCTTTTTACTAACCTTGCACTACTGTTAAAAGGATCCCCTATCTAGGGAATTTTCACTAAAAATTATACTGCCTTGTATGGAGCTCGATCTCCTTACGTTTCTTATATCATCTTTCTATTGAAAAAAGCTGTATGGTCGAATCTGCTAGAATGCTGGATATAATCTTGATTATGAGTCGTTAGCTGAATAGTGAAATAGTTCTGGTTGAAGAGGAGTAGCTAGTTGCTGATGGGTGATTGTTTGACTTTTGTATAACAATCTTAGAAGAAAAGGAACTGTTGTGACATCTTTACTCCAAAAGTAAGAGCTGTTCCGAAGTGAACTATGCACTATCAGGCTGAACTGATGCAGTTTTGATCAACCAATTTGAAAACAGAGGATCTGTTAGACATGTGATCCAACAAGAAATGTCCACATGCAGCATGGGACATAGGGGGTGTTCACGAAACTCAAGGAATTTTGTAATCGAACTGAATTGGAATGTGCATGGTTTGGATTTTGTTGGTTTTTATTTTTTAAAATGATTTTGGTTAGGTTTATCTAAATTTATATGAAGAAAATCAACTCTTCTTTTTTTTTTAGTTCAACAATAAATGGGATGAACGGATTCAAACTTCTAGCC

mRNA sequence

ATGGCCCACGGCGCAGAATCAAGGTGGCTGCCGCCCGCCATGAGCGTTACACTGGCGTTTATCTCCGCCTCTGCCTCCGCCATCATATCTATTTATCTAGCGACGAGGAAAACCAGAGAGTTGGATTCCAAGGTTCAAGAGCTCGAGACTTCTCTGGCAGCTTCTTTAAAGAAAACCGCTGCCGAGAGAAAAGGCCGAATACGGGCCCAGCAGGCTTTAAGGAAAGCACTTGCAGAACCAAAGTCTGATAGCGTGGAATGCACTTCGTATCCCATGACACCAATTGGTGTCATCCAGTCATCCTTCTCTACTAGGAATGGGACTCCAAGGCAATCTTTAATTGTACCTCTTGCGAGGGCACGGTTGGTGTTCTCTGCTCGTATTCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCACTGCTGGATTATATATGTCTTTCATTTGAATACAGATCTTGAGAAGCTGTGGAAGGATCCGTCTAAATCAAAATTCAAGGCAAAAGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCAATCGGACTCACTATTGCGAAGGTGGAGGCTGTCCAGGGACATCAAATTATACTCTCTGGTGCCGATCTTGTTGATGGAACTCCAGTTCTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAGGATGCAAAAGTCCCAGAGTGGTTAATGGGGGAAAATTTGTTGAGTGTTGCATCCATCTGTTTCTCTGAAGAATTTACTTCCACTCTATCGGAGTGTTGGCCACTAATGGAAAAGAAGTCGCTTTATTCATCCGCAGATGAGTTGAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCTATGTCACAACGTAGTCGGCCTCATGACTCCCTCGTTAAGACAACAACAGGACATGAACTGTCCGATGGTAGTTCAGATTCAGATGGTCACCTAGATGACAAAGTTTCGAGTGGTACCCAAAGTTCTTCTGGGAGCATTATCTATCACCTACTTGTAGATGGATTAGATGTGTCGTACACGATTGGTTGCCATGGTGACGTGGTTGTTGGCAAAGTAGTACTCTCAGCACCAAGCTCAAGAACAAGCAGCGATCAACACAAAGAAAACTCTTCTGAATAG

Coding sequence (CDS)

ATGGCCCACGGCGCAGAATCAAGGTGGCTGCCGCCCGCCATGAGCGTTACACTGGCGTTTATCTCCGCCTCTGCCTCCGCCATCATATCTATTTATCTAGCGACGAGGAAAACCAGAGAGTTGGATTCCAAGGTTCAAGAGCTCGAGACTTCTCTGGCAGCTTCTTTAAAGAAAACCGCTGCCGAGAGAAAAGGCCGAATACGGGCCCAGCAGGCTTTAAGGAAAGCACTTGCAGAACCAAAGTCTGATAGCGTGGAATGCACTTCGTATCCCATGACACCAATTGGTGTCATCCAGTCATCCTTCTCTACTAGGAATGGGACTCCAAGGCAATCTTTAATTGTACCTCTTGCGAGGGCACGGTTGGTGTTCTCTGCTCGTATTCCTCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCACTGCTGGATTATATATGTCTTTCATTTGAATACAGATCTTGAGAAGCTGTGGAAGGATCCGTCTAAATCAAAATTCAAGGCAAAAGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACTAGATCTCCACATAGACCATGCCCAATCGGACTCACTATTGCGAAGGTGGAGGCTGTCCAGGGACATCAAATTATACTCTCTGGTGCCGATCTTGTTGATGGAACTCCAGTTCTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAGGATGCAAAAGTCCCAGAGTGGTTAATGGGGGAAAATTTGTTGAGTGTTGCATCCATCTGTTTCTCTGAAGAATTTACTTCCACTCTATCGGAGTGTTGGCCACTAATGGAAAAGAAGTCGCTTTATTCATCCGCAGATGAGTTGAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCTATGTCACAACGTAGTCGGCCTCATGACTCCCTCGTTAAGACAACAACAGGACATGAACTGTCCGATGGTAGTTCAGATTCAGATGGTCACCTAGATGACAAAGTTTCGAGTGGTACCCAAAGTTCTTCTGGGAGCATTATCTATCACCTACTTGTAGATGGATTAGATGTGTCGTACACGATTGGTTGCCATGGTGACGTGGTTGTTGGCAAAGTAGTACTCTCAGCACCAAGCTCAAGAACAAGCAGCGATCAACACAAAGAAAACTCTTCTGAATAG

Protein sequence

MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTAAERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARARLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKVPEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMSQRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIGCHGDVVVGKVVLSAPSSRTSSDQHKENSSE

Homology

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BLAST of Spg007678 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 648.3 bits (1671), Expect = 4.3e-182
Identity = 325/382 (85.08%), Postives = 360/382 (94.24%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+ AESRW   A+SVTLAFI  SASA+IS+Y+ T+K+REL +KV+ELE SLA+SLKKTA
Sbjct: 1   MAYAAESRWSMAALSVTLAFI--SASAVISLYVVTKKSRELGTKVRELEASLASSLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSVECTSYPMTPIG I+SSFSTRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGLGEYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLTIAKVEA+QGHQI+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+NLL+VASICFSEEF  TLSECWPLMEKKSLY+S DE +NFVK+VLSWDIRS+S
Sbjct: 241 PKWLLGDNLLTVASICFSEEFIPTLSECWPLMEKKSLYASEDEFRNFVKEVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QR++PHDS VK TTGHE  DGSSDS+GHLDDKVSSG+QSSS +IIYHLLVDGLDVSYTIG
Sbjct: 301 QRNQPHDSHVKPTTGHECFDGSSDSEGHLDDKVSSGSQSSSENIIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
           CHG+VVVGKV L++PSSRT S+
Sbjct: 361 CHGNVVVGKVELASPSSRTRSN 380

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BLAST of Spg007678 vs. NCBI nr
Match: XP_022134721.1 (uncharacterized protein LOC111006924 [Momordica charantia])

HSP 1 Score: 646.7 bits (1667), Expect = 1.2e-181
Identity = 327/382 (85.60%), Postives = 357/382 (93.46%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+GAESRW   AMS+ LAFI  SASAI+S+YL TRK+REL SKV+ELE SLAASLKKT 
Sbjct: 1   MAYGAESRWSTAAMSIILAFI--SASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTT 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKALA+PKSD+VECTSYPMTPIGVIQS+FSTRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDL+KLWK+PSKS+FKAKVRVPRLKG+RV
Sbjct: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLTIAKVEA+QGHQI+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           PEWLMG+NLLSVASICFSEEFTSTLS+CWPLMEKKS Y+SADE KNFVKQVLSWDIRS+S
Sbjct: 241 PEWLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QR+RPH+S ++TTTGHELSDGSSD D HL DK S GTQ SS +IIYHLLVDGL+VSYTIG
Sbjct: 301 QRTRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
           CHGDVVV K  L++PSSRT S+
Sbjct: 361 CHGDVVVVKAELASPSSRTRSN 380

[Close]

BLAST of Spg007678 vs. NCBI nr
Match: TYK09463.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 629.4 bits (1622), Expect = 2.1e-176
Identity = 321/382 (84.03%), Postives = 352/382 (92.15%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+ AES W   AMSV L FI  SASAIIS+YL T+K+REL +KVQ+LE SLAASLKKTA
Sbjct: 1   MAYAAESIWSTAAMSVALTFI--SASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSVECTSYPMTPIG IQSSF+TRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGL EYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLT+AKVEAVQGH+I+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+N+L+VASICFSEEFTS LS+CWPL+EKKSLY+S +ELKNFVKQVLSWDIRS+S
Sbjct: 241 PKWLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QRSRPH S +KT TG EL D SSDSDGH+DDKVSS +QSSS S IYHLLVDGLDVSYTIG
Sbjct: 301 QRSRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
            HGDVVV KV L++PSSRT S+
Sbjct: 361 YHGDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of Spg007678 vs. NCBI nr
Match: XP_008453657.1 (PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.9e-175
Identity = 319/382 (83.51%), Postives = 351/382 (91.88%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+ AES W   AMSV L FI  SASAIIS+YL T+K+REL +KVQ+LE SLAASLKKTA
Sbjct: 1   MAYAAESIWSTAAMSVALTFI--SASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSVECTSYPMTPIG IQSSF+TRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGL EYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLT+AKVEAVQGH+I+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+++L+VASICFSEEFTS LS+CWPL+EKKSLY+S +ELKNFVKQVLSWDIRS+S
Sbjct: 241 PKWLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QRSRPH S +KT TG EL D SSDSDGH+DDKVSS +QS S S IYHLLVDGLDVSYTIG
Sbjct: 301 QRSRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
            HGDVVV KV L++PSSRT S+
Sbjct: 361 YHGDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of Spg007678 vs. NCBI nr
Match: XP_004149885.1 (uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical protein Csa_019626 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 1.1e-174
Identity = 319/382 (83.51%), Postives = 352/382 (92.15%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           M + A+SRW   AMSVTLAFI  SASAIIS+YL T+K+REL +KV++LE SLAASLKKTA
Sbjct: 1   MTYAADSRWSTAAMSVTLAFI--SASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSV+CTSYPMTPIG IQSSF+TRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGLGEYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLT+AKVEAVQGH+I+LSGADLVDGTPVLDVKPYLPYCDSVQ+A V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+N+L+VASICFSEEFTSTLS+CWPL EKKSLY+S DELKNFVKQVLSWDIRS+S
Sbjct: 241 PKWLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QRSRPH S +KT      SD SSDSDGHL+DKVSS +QSSS S IYHLLVDGLDVSYTIG
Sbjct: 301 QRSRPHCSHIKTD-----SDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
           CHGDVVV KV L++ SSRT S+
Sbjct: 361 CHGDVVVDKVELASSSSRTRSN 375

[Close]

BLAST of Spg007678 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 7.7e-33
Identity = 75/151 (49.67%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 94  PIGVIQSSFSTRNGTPRQSLIVPLARARLVFSARI---PPASIEGLGEYSHCWIIYVFHL 153
           P+G ++S FS +NGTPRQ  I   +RA L    RI   P  S+ GL ++SH WI++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 154 NTDLEKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAKVEAVQGHQII 213
           N  L            KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E V+G  I 
Sbjct: 92  NGHL----------SCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIY 151

Query: 214 LSGADLVDGTPVLDVKPYLPYCDSVQDAKVP 242
           LSG D++ GTPVLD+KPY+   DS Q+   P
Sbjct: 152 LSGIDMIHGTPVLDIKPYIAEYDSPQNVMEP 172

[Close]

BLAST of Spg007678 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 3.8e-32
Identity = 74/150 (49.33%), Postives = 95/150 (63.33%), Query Frame = 0


Query: 94  PIGVIQSSFSTRNGTPRQSLIVPLARARLVFSARI---PPASIEGLGEYSHCWIIYVFHL 153
           PIG ++S F  + GTPRQ  I   +RA L     I   P  S+ GL E+SH WI++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 154 NTDLEKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAKVEAVQGHQII 213
           N  L           +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E V+G  + 
Sbjct: 92  NGHL----------NYKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 214 LSGADLVDGTPVLDVKPYLPYCDSVQDAKV 241
           LSG D++DGTPVLD+KPY+   DS Q+  V
Sbjct: 152 LSGVDMIDGTPVLDIKPYIADYDSPQNLSV 171

[Close]

BLAST of Spg007678 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 5.5e-31
Identity = 72/147 (48.98%), Postives = 94/147 (63.95%), Query Frame = 0


Query: 94  PIGVIQSSFSTRNGTPRQSLIVPLARARLVFSARI---PPASIEGLGEYSHCWIIYVFHL 153
           PIG ++S FS + GTPRQ  I   +RA L     I   P  S+ GL ++SH WI++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 154 NTDLEKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTIAKVEAVQGHQII 213
           N  L           +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E V+G  + 
Sbjct: 92  NGHL----------NYKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 214 LSGADLVDGTPVLDVKPYLPYCDSVQD 238
           LSG D++ GTPVLD+KPY+   DS Q+
Sbjct: 152 LSGIDMIHGTPVLDIKPYIADYDSPQN 168

[Close]

BLAST of Spg007678 vs. ExPASy Swiss-Prot
Match: Q9RPT0 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rcsF PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.8e-27
Identity = 66/156 (42.31%), Postives = 97/156 (62.18%), Query Frame = 0


Query: 89  SYPMTPIGVIQSSFSTRNGTPRQSLIVPLARARLVFSARIPP----ASIEGLGEYSHCWI 148
           ++ ++PIG I+S F  +   PRQ L+ P AR  L     +PP     ++EGL + SH W+
Sbjct: 2   THSVSPIGYIRSCFMEKFAIPRQPLLAPAARGTLEL---LPPFDQVEALEGLEQVSHVWL 61

Query: 149 IYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGER-VGVFATRSPHRPCPIGLTIAKVEA 208
           +++FH             + K + KVR PRL G R +GVFATR+ HRP  IG ++ ++E 
Sbjct: 62  LFLFH----------QALEDKPRLKVRPPRLGGNRSLGVFATRATHRPNGIGQSVVRLEG 121

Query: 209 VQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAK 240
            +  ++ LSG DL+DGTPVLD+KPY+PY D+V DA+
Sbjct: 122 FEAGRLWLSGIDLLDGTPVLDIKPYVPYADAVADAR 144

[Close]

BLAST of Spg007678 vs. ExPASy Swiss-Prot
Match: O68638 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa OX=287 GN=rcsF PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.5e-25
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 0


Query: 89  SYPMTPIGVIQSSFSTRNGTPRQSLIVPLARARLVFSARIPP----ASIEGLGEYSHCWI 148
           S+ ++PIG ++S F  +   PRQ  + P A   L     +PP     ++EGL + SH W+
Sbjct: 2   SHSISPIGHVRSCFKEKFAIPRQPQLAPAATGVLEL---LPPFDTGDAVEGLEQVSHVWL 61

Query: 149 IYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKG-ERVGVFATRSPHRPCPIGLTIAKVEA 208
           I++FH             + K + KVR PRL G + +GVFATR+ HRP  +G ++ K+E 
Sbjct: 62  IFLFH----------QALEDKPRLKVRPPRLGGNQSMGVFATRATHRPNGLGQSVVKLEK 121

Query: 209 VQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDA 239
           V+  ++ LSG DL+DGTPV+D+KPY+PY D V DA
Sbjct: 122 VEPGRLWLSGIDLLDGTPVIDIKPYVPYADIVPDA 143

[Close]

BLAST of Spg007678 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.0e-182
Identity = 327/382 (85.60%), Postives = 357/382 (93.46%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+GAESRW   AMS+ LAFI  SASAI+S+YL TRK+REL SKV+ELE SLAASLKKT 
Sbjct: 1   MAYGAESRWSTAAMSIILAFI--SASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTT 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKALA+PKSD+VECTSYPMTPIGVIQS+FSTRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDL+KLWK+PSKS+FKAKVRVPRLKG+RV
Sbjct: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLTIAKVEA+QGHQI+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           PEWLMG+NLLSVASICFSEEFTSTLS+CWPLMEKKS Y+SADE KNFVKQVLSWDIRS+S
Sbjct: 241 PEWLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QR+RPH+S ++TTTGHELSDGSSD D HL DK S GTQ SS +IIYHLLVDGL+VSYTIG
Sbjct: 301 QRTRPHESHIETTTGHELSDGSSDLDTHLYDKASGGTQISSKNIIYHLLVDGLNVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
           CHGDVVV K  L++PSSRT S+
Sbjct: 361 CHGDVVVVKAELASPSSRTRSN 380

[Close]

BLAST of Spg007678 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.0e-176
Identity = 321/382 (84.03%), Postives = 352/382 (92.15%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+ AES W   AMSV L FI  SASAIIS+YL T+K+REL +KVQ+LE SLAASLKKTA
Sbjct: 1   MAYAAESIWSTAAMSVALTFI--SASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSVECTSYPMTPIG IQSSF+TRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGL EYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLT+AKVEAVQGH+I+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+N+L+VASICFSEEFTS LS+CWPL+EKKSLY+S +ELKNFVKQVLSWDIRS+S
Sbjct: 241 PKWLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QRSRPH S +KT TG EL D SSDSDGH+DDKVSS +QSSS S IYHLLVDGLDVSYTIG
Sbjct: 301 QRSRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSSSKSTIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
            HGDVVV KV L++PSSRT S+
Sbjct: 361 YHGDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of Spg007678 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.9e-175
Identity = 319/382 (83.51%), Postives = 351/382 (91.88%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+ AES W   AMSV L FI  SASAIIS+YL T+K+REL +KVQ+LE SLAASLKKTA
Sbjct: 1   MAYAAESIWSTAAMSVALTFI--SASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSVECTSYPMTPIG IQSSF+TRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGL EYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLT+AKVEAVQGH+I+LSGADLVDGTPVLDVKPYLPYCDSVQDA V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+++L+VASICFSEEFTS LS+CWPL+EKKSLY+S +ELKNFVKQVLSWDIRS+S
Sbjct: 241 PKWLLGDSVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QRSRPH S +KT TG EL D SSDSDGH+DDKVSS +QS S S IYHLLVDGLDVSYTIG
Sbjct: 301 QRSRPHCSHIKTVTGDELLDESSDSDGHVDDKVSSSSQSYSKSTIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
            HGDVVV KV L++PSSRT S+
Sbjct: 361 YHGDVVVDKVELASPSSRTRSN 380

[Close]

BLAST of Spg007678 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 5.5e-175
Identity = 319/382 (83.51%), Postives = 352/382 (92.15%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           M + A+SRW   AMSVTLAFI  SASAIIS+YL T+K+REL +KV++LE SLAASLKKTA
Sbjct: 1   MTYAADSRWSTAAMSVTLAFI--SASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTA 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA+AEPKSDSV+CTSYPMTPIG IQSSF+TRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFSARIPPASIEGLGEYSHCW+IYVFHLNTDL+KLWKDPSKSKFKAKVRVPRLKGERV
Sbjct: 121 RLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           GVFATRSPHRPCPIGLT+AKVEAVQGH+I+LSGADLVDGTPVLDVKPYLPYCDSVQ+A V
Sbjct: 181 GVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL+G+N+L+VASICFSEEFTSTLS+CWPL EKKSLY+S DELKNFVKQVLSWDIRS+S
Sbjct: 241 PKWLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIG 360
           QRSRPH S +KT      SD SSDSDGHL+DKVSS +QSSS S IYHLLVDGLDVSYTIG
Sbjct: 301 QRSRPHCSHIKTD-----SDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIG 360

Query: 361 CHGDVVVGKVVLSAPSSRTSSD 383
           CHGDVVV KV L++ SSRT S+
Sbjct: 361 CHGDVVVDKVELASSSSRTRSN 375

[Close]

BLAST of Spg007678 vs. ExPASy TrEMBL
Match: A0A6J1JCZ9 (uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.2e-171
Identity = 306/377 (81.17%), Postives = 347/377 (92.04%), Query Frame = 0


Query: 1   MAHGAESRWLPPAMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTA 60
           MA+   SR    AMS+TLAF+  SASAI+S+Y  T+K+REL SKV+ELE SLAASLKKT 
Sbjct: 1   MAYAVVSRSSTAAMSITLAFV--SASAILSLYFVTKKSRELGSKVRELEASLAASLKKTI 60

Query: 61  AERKGRIRAQQALRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARA 120
           AER+GRIRAQQALRKA++EPKSDSVECT YPM PIG IQSSFSTRNGTPRQSL+VPLARA
Sbjct: 61  AERQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARA 120

Query: 121 RLVFSARIPPASIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERV 180
           RLVFS+RIPPASIEGLGEYSHCW+IYVFHLNT+L+KLWKDPSKSKFKAKV+VPRLKGE+V
Sbjct: 121 RLVFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKV 180

Query: 181 GVFATRSPHRPCPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKV 240
           G+FATRSPHRPCPIG+TIAKVEA+QGHQI+LSGADLVDGTPVLDVKPYLPYCDS+QDA V
Sbjct: 181 GIFATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATV 240

Query: 241 PEWLMGENLLSVASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMS 300
           P+WL GEN+LSVASICFSEEFT +LSECWPL+EKKSLY+SADELK+FVKQVLSWDIRS+S
Sbjct: 241 PKWLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKSFVKQVLSWDIRSLS 300

Query: 301 QRSRPHDSLVKTTTGHELSDGSSDSDGHLDDKVSSGT-QSSSGSIIYHLLVDGLDVSYTI 360
           QRS+PH+S + TT GHELSDGSSDSDGHLD+ + SGT QSSS S++YHLLVDGL+VSYTI
Sbjct: 301 QRSQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTI 360

Query: 361 GCHGDVVVGKVVLSAPS 377
           GC GDVVV KV +++PS
Sbjct: 361 GCQGDVVVRKVEVASPS 375

[Close]

BLAST of Spg007678 vs. TAIR 10
Match: AT4G28020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0066 (InterPro:IPR001378); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-111
Identity = 204/361 (56.51%), Postives = 265/361 (73.41%), Query Frame = 0


Query: 13  AMSVTLAFISASASAIISIYLATRKTRELDSKVQELETSLAASLKKTAAERKGRIRAQQA 72
           A++ TLA +S SA+   S+Y   R+  +L+++++E E SL ++L+K+A+ERKGR++AQQA
Sbjct: 14  ALAFTLAVVSVSAA--FSLYRWRRREEDLENRIKEFEKSLGSTLEKSASERKGRVKAQQA 73

Query: 73  LRKALAEPKSDSVECTSYPMTPIGVIQSSFSTRNGTPRQSLIVPLARARLVFS-ARIPPA 132
           LR+ALA+ +S  +  T YPM PIG IQS FSTRNGTPRQ L+V LARA L+F  A +PPA
Sbjct: 74  LREALAQTESHDLHSTIYPMRPIGTIQSCFSTRNGTPRQPLLVSLARACLIFDPALVPPA 133

Query: 133 SIEGLGEYSHCWIIYVFHLNTDLEKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRP 192
           S+EGL EYSHCWI+YVFHLNTD+EKLW+ PS+SK KAKVRVPRL GER GVFATRSPHRP
Sbjct: 134 SLEGLEEYSHCWILYVFHLNTDIEKLWRKPSQSKLKAKVRVPRLNGERKGVFATRSPHRP 193

Query: 193 CPIGLTIAKVEAVQGHQIILSGADLVDGTPVLDVKPYLPYCDSVQDAKVPEWLMGENLLS 252
           CPIGLT+AKVE +Q  +++LSG DLVDGTPVLD+KPYLPY DS+Q A VP W+  +  L+
Sbjct: 194 CPIGLTVAKVEEIQKDKVLLSGVDLVDGTPVLDIKPYLPYSDSIQGASVPNWVKEDCSLA 253

Query: 253 VASICFSEEFTSTLSECWPLMEKKSLYSSADELKNFVKQVLSWDIRSMSQRSRPHDSLVK 312
           VAS+ FS+ F+S+++ CW L+EKKSLYSSADE ++ + QVLSWDIRSMSQR++P D+L  
Sbjct: 254 VASVTFSDTFSSSITSCWKLIEKKSLYSSADEFRSLITQVLSWDIRSMSQRNKPQDTL-- 313

Query: 313 TTTGHELSDGSSDSDGHLDDKVSSGTQSSSGSIIYHLLVDGLDVSYTIGCHGDVVVGKVV 372
                                           I+YHL+++GLDVSY I    +++V  V 
Sbjct: 314 -----------------------------DEEIVYHLVLEGLDVSYMIDNESNILVQDVS 341

The following BLAST results are available for this feature:

BLAST of Spg007678 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879626.1	4.3e-182	85.08	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida]	[more]
XP_022134721.1	1.2e-181	85.60	uncharacterized protein LOC111006924 [Momordica charantia]	[more]
TYK09463.1	2.1e-176	84.03	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008453657.1	3.9e-175	83.51	PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo]	[more]
XP_004149885.1	1.1e-174	83.51	uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical ...	[more]

BLAST of Spg007678 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9BU70	7.7e-33	49.67	tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=...	[more]
Q562D6	3.8e-32	49.33	tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV...	[more]
Q4V7E0	5.5e-31	48.98	tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE...	[more]
Q9RPT0	2.8e-27	42.31	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]
O68638	4.5e-25	41.29	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]

BLAST of Spg007678 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYL5	6.0e-182	85.60	uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A5D3CC84	1.0e-176	84.03	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BW92	1.9e-175	83.51	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LTM6	5.5e-175	83.51	TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P...	[more]
A0A6J1JCZ9	2.2e-171	81.17	uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678...	[more]

BLAST of Spg007678 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28020.1	1.4e-111	56.51	unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023370	TrmO-like, N-terminal domain	TIGRFAM	TIGR00104	TIGR00104	coord: 90..239 e-value: 2.8E-29 score: 100.1
IPR023370	TrmO-like, N-terminal domain	PFAM	PF01980	TrmO	coord: 107..233 e-value: 7.0E-43 score: 145.6
IPR023370	TrmO-like, N-terminal domain	PROSITE	PS51668	TSAA_2	coord: 92..237 score: 67.398773
IPR023370	TrmO-like, N-terminal domain	CDD	cd09281	UPF0066	coord: 94..228 e-value: 2.21397E-49 score: 161.12
IPR036414	YaeB, N-terminal domain superfamily	GENE3D	2.40.30.70		coord: 91..246 e-value: 1.7E-53 score: 182.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 305..339
None	No IPR available	PANTHER	PTHR12818:SF0	TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE	coord: 22..373
IPR040372	YaeB-like	PANTHER	PTHR12818	UNCHARACTERIZED	coord: 22..373
IPR036413	YaeB-like superfamily	SUPERFAMILY	118196	YaeB-like	coord: 88..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg007678.1	Spg007678.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016430	tRNA (adenine-N6-)-methyltransferase activity