Spg007596 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg007596
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein SIEVE ELEMENT OCCLUSION B-like
Locationscaffold2: 812723 .. 816192 (-)
RNA-Seq ExpressionSpg007596
SyntenySpg007596
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCTTTGTTTTTATAGCTTTCTCTTTTCATGTAACACACACAGCTTCCTTTCTTCTAATTGGCACATTTATCCACAAACCCATTTCATTTTAAGATAATATGTTTTCCTAAAAGTGATAAAGTATCAGCCTGCTCATCTGGATTATGATAATTCCCAACTTTAACAACTAATTCCAAAGCTTCTTTTGGCCCAAAGAAGAACAAAGACTTGCTCAATTCTCCCTCAGTTCCCTCACTGACCAATGCCATTTCAGCCCTTCACAATATGAACCAGACAATCCAACTCCCTTTATGAATATATATATTGCATGGAGTAACCAGCTTTAGCTTTTGTTTCAACTCCAGTTGCAAGCAAGGAACTACTTCTACATTGCTTTATTCCATCTCATAATGTTATATAAACCTCTATCTACCAAATTCTAAGTCACCAAACCAAAACTTCTCTACTGATATCTTGTTCTTTTCCAGCTAACTCTTGTGTGTTCAGTTTTGATATGGCAGCCCTCACTCGCCCAGCTCCCAAAATGAATCAGCTGATCAAAAATGACAAGAGAATGTTGTCTGCCACTGATGACAATGCAATGACTAAGCAAGTTCTCGCAACTCATGCTCCGGACGGGCGCGAGGTCTTTGTCAGGCCGATTCTGCTCACTGTCGAGGACATTATCCGCCGTGCCACACCCGATATCGGAAAAGTTCTTGATGTGAAACCTCTCTGATTTGCTCCATTTGGCCTTCTTTCCCATCTACTTAAGACTGATGTGGTGTGGTGAAATGATGCAGGGCAATGGCCAGCATGAGGATATGTTGGAGGACCAAATCAGTTCTGCAGAAATGGAAGGGATGCTCGAATCGTTGGCTCATGTCATACAAAAAGTCGGCGGCGAGGTCGAATATATATGCATTTGCTCTTTGAAAGTTTTTGAATTTTAGTCCTCTATCACCATTTCTGCCATGTTTAAGATGTAAAGGCTCTACTTCTTTTTGAACCTTTTTGAGACTGAGAACTTTTGAACTTTTGATGTTTCTGTTGATGGCAGCTAGCATGTAAGTGCTCAGGTGGAGATGCACATGCCACAACTATGGCTATATTGAACTTACTGTCAAATTACTCATGGGATGCAAAAGTGGTGATCACAATGGCTGCCTTTGCTGTGAACTATGGACAGTATTGGCTTTTGGCTCAACTTTACACAACCAATATGCTAGCCAAGGCTCTGGCTCTGCTGAAGCAACTGCCTGATGTTATAGAGCATTCCAATTCATTGAAGCCCCAATTTGATGCGCTTAACAAGCTCATCAAGGCTATGTTGGACGTAACGAAGTGCATTGTCGAGTTCACCGAGCTACCATCTCAATACATTTCACAGGACACGCCTGCAATGTCGGTTGCCATAGCTCATTTTCCTACAGCTGCCTATTGGACCATCAAAAGTGCGGTTGCTTGTACGTCGCTGATCGAAAGCCTCGTCAGCTTGAGCCATGAGTGAGTGATGCATTGCAATATACTCAGCAACATCATTGTAAAGCTTGAGCCTAATTCATAGTCAGGAATCAACTCTTTTTCTCTGATATGTTTCAGGCGGATTATGTCGACCACCGAAGTATGGGAACTTTCCAGCTTGGCTCACAAGGAAACAAATATATATGAGCATCTCCGGACGCAGCTGGATCTTTGCTTACAACACATAGGTGAGAAAGCAAACATTTGATAGCAATTTGCATACCTTTAAGTATATGCTGAAATGATAAACACTATGTGATGCAGATGGGAAAAGGCATTTGGAGGCCTATCAGAATCTTGTGAGAATTTCAGAAACTCTTCACTTAGACAACATGAAGCTTATCAGGGCTTTAATTTCTACAAGAGATGACATACAGCCTCTCTACGATGGCACCTCAAAAACGACGGTTTGTTCACAAATCCAATATACTTCAACTGCTACTCTGTTGAATCTAACTTCAAACTGAAGAGGAGGCTTTTTTCTGTTGGCACAAGCAGGTTCACCTTGAGATATTGAAGAGGAAACATGTGTTACTGTTGATATCAGATCTTGATATCACCCATGAAGAGGTTATGATTCTCGACAACCTATTCAAAGAGTCGCAGCAGCGACCGGAAATTCGATATGAGATAGTGTGGATACCAATCATCGATCATTCAATCGAACAGCACAACAAGAACCAACACAAGTTTAAGCAGCTGCAACAGCTGATGCCATGGTTCAGTGTGCATGACCCATCAATAATTGAGCTCTCAGTCGTAAGGTTCATCAAAGAAAAATGGAACTTCACAAAGAAAACAATCTTAGTGGCCTTAGATCCACAAGGAAGGGTGTCTTCCACCAATGCCCTTCACATGCTTTGGATATGGGGAAATCTAGCCTTCCCCTTCACTTCAGAGAGAGAAGAAGCACTCTGGAAAACAGAGAGCTGGAGGCTCGAACTTCTCATAGATGGCATAGATCTATCCATTCTCGATTGGGTATCTCCATTTTCCCAAATTTTTCATCACATTCTCCAAAACCCCATCTCATAAAACTTGCAAAGTTTCAAGAACAATTGACAAAAGGGGTTTGTTTCTTTTTGTTTCTTTTCCATCAGGCGGCAGAAGGAAGATACATATGCTTATTTGGAGGGGAAGACATGGAGTGGATCAAGGACTTCACAATCAAAACAAAACAAGTAGCAGAAGCAGCAAAAGTAGACTTACAAATGACGTATGTAGGGAAGAACAATGCCAAAGAACGAGTCAGGAAAATCACCATCATGATTGGAGACAACAAGCTGAGCCATTACTGGCCAGATTCCACACTGGTCTGGTTCTTCTGGGCACGATTAGAGAGCATGATGTACTCAAAACTAAACTATGGAAAAACAGTTGAAAATGACCAAATAATGCAAGAGATCATGACCCTTTTGAGCTTCGATGGAAGTGACAAAGGATGGGCCATTTTCTTTGGAAGAGGAGGGGAAATGACAAGAGCCAAAGGGGAAATTGCTCTGAGTTGCATATTGGCATTCGAAGAGTGGAAAGACGAGGTGGAAGAGAAGGGCTTTTTGACGGCATTGGCCGAGTATCTGCAGCAACTGAGAACCCCACATCACTGCAACCGCCTGATTCTGCCGGGGCTCGCCGGAAACATACCGGAAAATGTGGTGTGTGCAGAGTGTGGACGGGCCATGGAGAAGTATCTGATGTACCGATGCTGTGTTGAATGAGAAAGCAAAAGGGAAGAAGGAATATAGACTTGTGTTTGTTTGTTTTTTTTTTTTTAGTTTTGTAATTTGGATGTTTGTTTTGATTGGAAAAGTGCTTAGCTTACTACTTTGATTTGATTTTGTTGCTTCTTCTTAAGAGATCTCTGCATGCACCATTTTGGTGTAAAAAGAGGAATGTCTTTTGATTGTGTGATTTGAAGTTACTTTTCTTGTAAGTTGTGAGAACAAACCATAAATAAAGTTCTAT

mRNA sequence

ATGGCAGCCCTCACTCGCCCAGCTCCCAAAATGAATCAGCTGATCAAAAATGACAAGAGAATGTTGTCTGCCACTGATGACAATGCAATGACTAAGCAAGTTCTCGCAACTCATGCTCCGGACGGGCGCGAGGTCTTTGTCAGGCCGATTCTGCTCACTGTCGAGGACATTATCCGCCGTGCCACACCCGATATCGGAAAAGTTCTTGATGGCAATGGCCAGCATGAGGATATGTTGGAGGACCAAATCAGTTCTGCAGAAATGGAAGGGATGCTCGAATCGTTGGCTCATGTCATACAAAAAGTCGGCGGCGAGGTCGAATATATATGCATTTGCTCTTTGAAACTAGCATGTAAGTGCTCAGGTGGAGATGCACATGCCACAACTATGGCTATATTGAACTTACTGTCAAATTACTCATGGGATGCAAAAGTGGTGATCACAATGGCTGCCTTTGCTGTGAACTATGGACAGTATTGGCTTTTGGCTCAACTTTACACAACCAATATGCTAGCCAAGGCTCTGGCTCTGCTGAAGCAACTGCCTGATGTTATAGAGCATTCCAATTCATTGAAGCCCCAATTTGATGCGCTTAACAAGCTCATCAAGGCTATGTTGGACGTAACGAAGTGCATTGTCGAGTTCACCGAGCTACCATCTCAATACATTTCACAGGACACGCCTGCAATGTCGGTTGCCATAGCTCATTTTCCTACAGCTGCCTATTGGACCATCAAAAGTGCGGTTGCTTGTACGTCGCTGATCGAAAGCCTCGTCAGCTTGAGCCATGAGCGGATTATGTCGACCACCGAAGTATGGGAACTTTCCAGCTTGGCTCACAAGGAAACAAATATATATGAGCATCTCCGGACGCAGCTGGATCTTTGCTTACAACACATAGATGGGAAAAGGCATTTGGAGGCCTATCAGAATCTTGTGAGAATTTCAGAAACTCTTCACTTAGACAACATGAAGCTTATCAGGGCTTTAATTTCTACAAGAGATGACATACAGCCTCTCTACGATGGCACCTCAAAAACGACGGTTCACCTTGAGATATTGAAGAGGAAACATGTGTTACTGTTGATATCAGATCTTGATATCACCCATGAAGAGGTTATGATTCTCGACAACCTATTCAAAGAGTCGCAGCAGCGACCGGAAATTCGATATGAGATAGTGTGGATACCAATCATCGATCATTCAATCGAACAGCACAACAAGAACCAACACAAGTTTAAGCAGCTGCAACAGCTGATGCCATGGTTCAGTGTGCATGACCCATCAATAATTGAGCTCTCAGTCGTAAGGTTCATCAAAGAAAAATGGAACTTCACAAAGAAAACAATCTTAGTGGCCTTAGATCCACAAGGAAGGGTGTCTTCCACCAATGCCCTTCACATGCTTTGGATATGGGGAAATCTAGCCTTCCCCTTCACTTCAGAGAGAGAAGAAGCACTCTGGAAAACAGAGAGCTGGAGGCTCGAACTTCTCATAGATGGCATAGATCTATCCATTCTCGATTGGGCGGCAGAAGGAAGATACATATGCTTATTTGGAGGGGAAGACATGGAGTGGATCAAGGACTTCACAATCAAAACAAAACAAGTAGCAGAAGCAGCAAAAGTAGACTTACAAATGACGTATGTAGGGAAGAACAATGCCAAAGAACGAGTCAGGAAAATCACCATCATGATTGGAGACAACAAGCTGAGCCATTACTGGCCAGATTCCACACTGGTCTGGTTCTTCTGGGCACGATTAGAGAGCATGATGTACTCAAAACTAAACTATGGAAAAACAGTTGAAAATGACCAAATAATGCAAGAGATCATGACCCTTTTGAGCTTCGATGGAAGTGACAAAGGATGGGCCATTTTCTTTGGAAGAGGAGGGGAAATGACAAGAGCCAAAGGGGAAATTGCTCTGAGTTGCATATTGGCATTCGAAGAGTGGAAAGACGAGGTGGAAGAGAAGGGCTTTTTGACGGCATTGGCCGAGTATCTGCAGCAACTGAGAACCCCACATCACTGCAACCGCCTGATTCTGCCGGGGCTCGCCGGAAACATACCGGAAAATGTGGTGTGTGCAGAGTGTGGACGGGCCATGGAGAAGTATCTGATGTACCGATGCTGTGTTGAATGA

Coding sequence (CDS)

ATGGCAGCCCTCACTCGCCCAGCTCCCAAAATGAATCAGCTGATCAAAAATGACAAGAGAATGTTGTCTGCCACTGATGACAATGCAATGACTAAGCAAGTTCTCGCAACTCATGCTCCGGACGGGCGCGAGGTCTTTGTCAGGCCGATTCTGCTCACTGTCGAGGACATTATCCGCCGTGCCACACCCGATATCGGAAAAGTTCTTGATGGCAATGGCCAGCATGAGGATATGTTGGAGGACCAAATCAGTTCTGCAGAAATGGAAGGGATGCTCGAATCGTTGGCTCATGTCATACAAAAAGTCGGCGGCGAGGTCGAATATATATGCATTTGCTCTTTGAAACTAGCATGTAAGTGCTCAGGTGGAGATGCACATGCCACAACTATGGCTATATTGAACTTACTGTCAAATTACTCATGGGATGCAAAAGTGGTGATCACAATGGCTGCCTTTGCTGTGAACTATGGACAGTATTGGCTTTTGGCTCAACTTTACACAACCAATATGCTAGCCAAGGCTCTGGCTCTGCTGAAGCAACTGCCTGATGTTATAGAGCATTCCAATTCATTGAAGCCCCAATTTGATGCGCTTAACAAGCTCATCAAGGCTATGTTGGACGTAACGAAGTGCATTGTCGAGTTCACCGAGCTACCATCTCAATACATTTCACAGGACACGCCTGCAATGTCGGTTGCCATAGCTCATTTTCCTACAGCTGCCTATTGGACCATCAAAAGTGCGGTTGCTTGTACGTCGCTGATCGAAAGCCTCGTCAGCTTGAGCCATGAGCGGATTATGTCGACCACCGAAGTATGGGAACTTTCCAGCTTGGCTCACAAGGAAACAAATATATATGAGCATCTCCGGACGCAGCTGGATCTTTGCTTACAACACATAGATGGGAAAAGGCATTTGGAGGCCTATCAGAATCTTGTGAGAATTTCAGAAACTCTTCACTTAGACAACATGAAGCTTATCAGGGCTTTAATTTCTACAAGAGATGACATACAGCCTCTCTACGATGGCACCTCAAAAACGACGGTTCACCTTGAGATATTGAAGAGGAAACATGTGTTACTGTTGATATCAGATCTTGATATCACCCATGAAGAGGTTATGATTCTCGACAACCTATTCAAAGAGTCGCAGCAGCGACCGGAAATTCGATATGAGATAGTGTGGATACCAATCATCGATCATTCAATCGAACAGCACAACAAGAACCAACACAAGTTTAAGCAGCTGCAACAGCTGATGCCATGGTTCAGTGTGCATGACCCATCAATAATTGAGCTCTCAGTCGTAAGGTTCATCAAAGAAAAATGGAACTTCACAAAGAAAACAATCTTAGTGGCCTTAGATCCACAAGGAAGGGTGTCTTCCACCAATGCCCTTCACATGCTTTGGATATGGGGAAATCTAGCCTTCCCCTTCACTTCAGAGAGAGAAGAAGCACTCTGGAAAACAGAGAGCTGGAGGCTCGAACTTCTCATAGATGGCATAGATCTATCCATTCTCGATTGGGCGGCAGAAGGAAGATACATATGCTTATTTGGAGGGGAAGACATGGAGTGGATCAAGGACTTCACAATCAAAACAAAACAAGTAGCAGAAGCAGCAAAAGTAGACTTACAAATGACGTATGTAGGGAAGAACAATGCCAAAGAACGAGTCAGGAAAATCACCATCATGATTGGAGACAACAAGCTGAGCCATTACTGGCCAGATTCCACACTGGTCTGGTTCTTCTGGGCACGATTAGAGAGCATGATGTACTCAAAACTAAACTATGGAAAAACAGTTGAAAATGACCAAATAATGCAAGAGATCATGACCCTTTTGAGCTTCGATGGAAGTGACAAAGGATGGGCCATTTTCTTTGGAAGAGGAGGGGAAATGACAAGAGCCAAAGGGGAAATTGCTCTGAGTTGCATATTGGCATTCGAAGAGTGGAAAGACGAGGTGGAAGAGAAGGGCTTTTTGACGGCATTGGCCGAGTATCTGCAGCAACTGAGAACCCCACATCACTGCAACCGCCTGATTCTGCCGGGGCTCGCCGGAAACATACCGGAAAATGTGGTGTGTGCAGAGTGTGGACGGGCCATGGAGAAGTATCTGATGTACCGATGCTGTGTTGAATGA

Protein sequence

MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKCSGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQLPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAAYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHIDGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVLLLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLMPWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFTSEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEAAKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGKTVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKGFLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Homology
BLAST of Spg007596 vs. NCBI nr
Match: XP_023002120.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita maxima])

HSP 1 Score: 1298.5 bits (3359), Expect = 0.0e+00
Identity = 640/714 (89.64%), Postives = 676/714 (94.68%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MA+ TRPAP M++LIKND+RMLSA+DDNAMTKQ+LATHAPDGR V  +PIL T+ED+IRR
Sbjct: 1   MASHTRPAPNMHKLIKNDRRMLSASDDNAMTKQILATHAPDGRMVDFKPILFTIEDVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATP IG VLDGNGQHEDMLED ISSAEM+G+LESL +VIQKVG E          LACKC
Sbjct: 61  ATPAIGNVLDGNGQHEDMLEDHISSAEMDGVLESLTYVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHA+TMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHASTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDV+E+S+SLKPQF+ALNKL+KAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA
Sbjct: 181 LPDVMENSSSLKPQFEALNKLVKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
            YWTIKSAVACTSLIESLVSL+HERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI
Sbjct: 241 TYWTIKSAVACTSLIESLVSLTHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           DGK+HLEAYQNLVRISET HLDNMK IRALIS RDDIQPLYDGTSKTTVHLEILKRKHVL
Sbjct: 301 DGKKHLEAYQNLVRISETHHLDNMKFIRALISARDDIQPLYDGTSKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI+HEEVM+LDNLFKESQQR EIRYEIVWIPIID SIEQ  KN+H FK++Q +M
Sbjct: 361 LLISDLDISHEEVMVLDNLFKESQQRQEIRYEIVWIPIIDQSIEQQGKNKHTFKEVQMMM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSVH+PSIIE SV+RFIKEKWNFTKKTILVALDPQG+VSSTNALHMLWIWGN AFPFT
Sbjct: 421 PWFSVHEPSIIERSVIRFIKEKWNFTKKTILVALDPQGKVSSTNALHMLWIWGNQAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIK+FT+KTKQVAE 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKEFTMKTKQVAEE 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           AKVDLQM YVGKNNAKERVRKITIMIG+NKLSHYWPDSTL+WFFWARLESMMYSKLN+G+
Sbjct: 541 AKVDLQMAYVGKNNAKERVRKITIMIGENKLSHYWPDSTLIWFFWARLESMMYSKLNHGR 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGE AL CILA+EEWK EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGETALGCILAYEEWKTEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 704

BLAST of Spg007596 vs. NCBI nr
Match: XP_023530744.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1293.9 bits (3347), Expect = 0.0e+00
Identity = 637/714 (89.22%), Postives = 674/714 (94.40%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MA+ TR AP M++LIKND+RMLSA+DDNAMTKQ+LATHAPDGR V  +PIL T+ED+IRR
Sbjct: 1   MASHTRSAPNMHKLIKNDRRMLSASDDNAMTKQILATHAPDGRMVDFKPILFTIEDVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATPDIG VLDGNGQHEDMLED I SAEM+G LESL +VIQKVG E          LACKC
Sbjct: 61  ATPDIGNVLDGNGQHEDMLEDHIGSAEMDGALESLTYVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHA+TMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHASTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDV+E+S+SLKPQF+ALNKL+KAM+DVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA
Sbjct: 181 LPDVMENSSSLKPQFEALNKLVKAMMDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
            YWTIKSAVACTSLIESLVSL+HERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI
Sbjct: 241 TYWTIKSAVACTSLIESLVSLTHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           DGK+HLEAYQNLVRISET HLDNMK IRALIS RDDIQPLYDGTSKTTVHLEILKRKHVL
Sbjct: 301 DGKKHLEAYQNLVRISETHHLDNMKFIRALISARDDIQPLYDGTSKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI+HEEVM+LDNLFKESQQR EIRYEIVWIPIID SIEQ  KN+H FK++Q +M
Sbjct: 361 LLISDLDISHEEVMVLDNLFKESQQRQEIRYEIVWIPIIDQSIEQQGKNKHTFKEVQMMM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSVH+PSIIE SV+RFIKEKWNFTKKTILVALDPQG+VSSTNALHMLWIWGN AFPFT
Sbjct: 421 PWFSVHEPSIIERSVIRFIKEKWNFTKKTILVALDPQGKVSSTNALHMLWIWGNQAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIK+FT+KTKQVA+ 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKEFTMKTKQVAKE 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           AKVDLQM YVGKNNAKERVRKITIMIG+NKLSHYWPDSTL+WFFWARLESMMYSKLN+G+
Sbjct: 541 AKVDLQMAYVGKNNAKERVRKITIMIGENKLSHYWPDSTLIWFFWARLESMMYSKLNHGR 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGE AL CILA+EEWK EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGETALGCILAYEEWKTEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 704

BLAST of Spg007596 vs. NCBI nr
Match: KAG6587435.1 (Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021418.1 Protein SIEVE ELEMENT OCCLUSION B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 638/714 (89.36%), Postives = 671/714 (93.98%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MA+ TRP P M++LIKND+RMLSA+DDNAMTKQ+LATHAPDGR V  +PIL T+ED+IRR
Sbjct: 1   MASHTRPPPNMHKLIKNDRRMLSASDDNAMTKQILATHAPDGRMVDFKPILFTIEDVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATP IG VLDGNGQHEDMLED I SAEM+G LESL +VIQKVG E          LACKC
Sbjct: 61  ATPAIGNVLDGNGQHEDMLEDHIGSAEMDGALESLTYVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHA+TMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHASTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDV+E+S SLKPQF+ALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA
Sbjct: 181 LPDVMENSTSLKPQFEALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
            YWTIKSAVACTSLIESLVSL+HERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI
Sbjct: 241 TYWTIKSAVACTSLIESLVSLTHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           DGK+HLEAYQNLVRISET HLDNMK IRALIS RDDIQPLYDGT KTTVHLEILKRKHVL
Sbjct: 301 DGKKHLEAYQNLVRISETHHLDNMKFIRALISARDDIQPLYDGTLKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI+HEEVM+LDNLFKESQQR EIRYEIVWIPIID SIEQ  KN+H FK++Q +M
Sbjct: 361 LLISDLDISHEEVMVLDNLFKESQQRQEIRYEIVWIPIIDQSIEQQGKNKHTFKEVQMMM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSVH+PSIIE SV+RFIKEKWNFTKKTILVALDPQG+VSSTNALHMLWIWGN AFPFT
Sbjct: 421 PWFSVHEPSIIERSVIRFIKEKWNFTKKTILVALDPQGKVSSTNALHMLWIWGNQAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIK+FT+KTKQVAE 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKEFTMKTKQVAEE 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           AKVDLQM YVGKNNAKERVRKITIMIG+NKLSHYWPDSTL+WFFWARLESMMYSKLN+G+
Sbjct: 541 AKVDLQMAYVGKNNAKERVRKITIMIGENKLSHYWPDSTLIWFFWARLESMMYSKLNHGR 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGE AL CILA+EEWK EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGETALGCILAYEEWKTEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 704

BLAST of Spg007596 vs. NCBI nr
Match: XP_022926283.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita moschata])

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 638/714 (89.36%), Postives = 671/714 (93.98%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MA+ TRP P M++LIKND+RMLSA+DDNAMTKQ+LATHAPDGR V  +PIL T+ED+IRR
Sbjct: 1   MASHTRPPPNMHKLIKNDRRMLSASDDNAMTKQILATHAPDGRMVDFKPILFTIEDVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATP IG VLDGNGQHEDMLED I S EM+G LESL +VIQKVG E          LACKC
Sbjct: 61  ATPAIGNVLDGNGQHEDMLEDHIGSPEMDGALESLTYVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHA+TMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHASTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDV+E+S SLKPQF+ALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA
Sbjct: 181 LPDVMENSTSLKPQFEALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
           AYWTIKSAVACTSLIESLVSL+HERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI
Sbjct: 241 AYWTIKSAVACTSLIESLVSLTHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           DGK+HLEAYQNLVRISET HLDNMK IRALIS RDDIQPLYDGTSKTTVHLEILKRKHVL
Sbjct: 301 DGKKHLEAYQNLVRISETHHLDNMKFIRALISARDDIQPLYDGTSKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISD DI+HEEVM+LDNLFKESQQR EIRYEIVWIPIID SIEQ  KN+H FK++Q +M
Sbjct: 361 LLISDRDISHEEVMVLDNLFKESQQRQEIRYEIVWIPIIDQSIEQQGKNKHTFKEVQMMM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSVH+PSIIE SV+RFIKEKWNFTKKTILVALDPQG+VSSTNALHMLWIWGN AFPFT
Sbjct: 421 PWFSVHEPSIIERSVIRFIKEKWNFTKKTILVALDPQGKVSSTNALHMLWIWGNQAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIK+FT+KTKQVAE 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKEFTMKTKQVAEE 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           AKVDLQM YVGKNNAKERVRKITIMIG+NKLSHYWPDSTL+WFFWARLESMMYSKLN+G+
Sbjct: 541 AKVDLQMAYVGKNNAKERVRKITIMIGENKLSHYWPDSTLIWFFWARLESMMYSKLNHGR 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGE AL CILA+EEWK EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGETALGCILAYEEWKTEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 704

BLAST of Spg007596 vs. NCBI nr
Match: XP_038880148.1 (protein SIEVE ELEMENT OCCLUSION B-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 633/714 (88.66%), Postives = 671/714 (93.98%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MAA TRP PK +QL KND+RMLSA+DDNAMTKQV+ATHAPDGREV V+PILL VE++IRR
Sbjct: 1   MAAYTRPVPKTHQLAKNDRRMLSASDDNAMTKQVVATHAPDGREVNVKPILLIVEEVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATPDIGKV  GNGQHEDM EDQISSAEM+G+LE LAHVIQKVG E          LACKC
Sbjct: 61  ATPDIGKV--GNGQHEDMFEDQISSAEMDGVLEPLAHVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLL+QLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLSQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDVIEHSNSLKPQFDAL+KLI A+L+VTKCIV+FTELPS YIS DTPAMSVA+AHFPTA
Sbjct: 181 LPDVIEHSNSLKPQFDALSKLITAVLNVTKCIVKFTELPSHYISPDTPAMSVALAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
           AYWTIKS VACTSLIES VSLSHE I+STTEVWELSSLAHKETNI+EHL+TQLDLCLQHI
Sbjct: 241 AYWTIKSVVACTSLIESFVSLSHELIISTTEVWELSSLAHKETNIHEHLQTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           D KRHLEAYQNLVRISETLHLDNMK IRALIS R+DIQPLYDGTSK TVHLEILKRKHVL
Sbjct: 301 DEKRHLEAYQNLVRISETLHLDNMKFIRALISAREDIQPLYDGTSKLTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI+HEEVMI+DNLFKESQQRPEIRYEIVWIPIID SI+QH+KN+HKF++L+QLM
Sbjct: 361 LLISDLDISHEEVMIVDNLFKESQQRPEIRYEIVWIPIIDRSIQQHSKNKHKFEELKQLM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PW+SV+DPSIIE SV+R+IKEKWNF KKTILVALDPQG+VSSTNALHMLWIWGNLAFPFT
Sbjct: 421 PWYSVYDPSIIEQSVIRYIKEKWNFKKKTILVALDPQGKVSSTNALHMLWIWGNLAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYIC+FGGED EWIK+FT KTKQVAEA
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICIFGGEDTEWIKEFTSKTKQVAEA 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           A VDLQM YVGKNNAKERVRKI+IMI DNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK
Sbjct: 541 ANVDLQMAYVGKNNAKERVRKISIMIADNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEM RAKGE ALSCILAF++WK+EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMARAKGETALSCILAFDQWKEEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FL ALAEYLQQLRTPHHCNRLILPGL GNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLKALAEYLQQLRTPHHCNRLILPGLTGNIPENVVCAECGRAMEKYLMYRCCVE 702

BLAST of Spg007596 vs. ExPASy Swiss-Prot
Match: Q9SS87 (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.4e-157
Identity = 291/710 (40.99%), Postives = 440/710 (61.97%), Query Frame = 0

Query: 24  ATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQHEDML---- 83
           ++D++ M K +  TH+PD REV VR +L  VEDI+ RAT      LD    +  ML    
Sbjct: 37  SSDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRAT------LDSEDTNASMLPLPT 96

Query: 84  EDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKCSGGDAHATTMAILNLLSNY 143
           ED++  + M  +L+S+++ I +V  E+ Y  +         +G D+H  TM++   LS++
Sbjct: 97  EDKLMQSSMMSVLDSVSYAIDRVACEIAYKSL---------TGSDSHEITMSVFEHLSSF 156

Query: 144 SWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQLPDVIEHSNSLKPQFDALN 203
            WD K+V+T+AAFA+NYG++WLL Q Y+ N LAK+LA+LK +P  +++  +L+     LN
Sbjct: 157 QWDGKLVLTLAAFALNYGEFWLLVQFYSKNQLAKSLAMLKLVP--VQNRVTLESVSQGLN 216

Query: 204 KLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAAYWTIKSAVACTSLIESLV 263
            LI+ M  VT C+VE +ELP +YI+ D P +S  ++  P A YWTI+S +AC S I  + 
Sbjct: 217 DLIREMKSVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMIT 276

Query: 264 SLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHIDGKRHLEAYQNLVRISETL 323
           ++ HE + +  ++WE S LA+K  NI++HL   L LC +HI+ +R  E+ + L  + +T 
Sbjct: 277 AMGHEMMNTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTT 336

Query: 324 HLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVLLLISDLDITHEEVMILDNL 383
           H+DNMK++ AL+  +  I PL DG +K  VHL++L+RK VLLLISDL+I  +E+ I + +
Sbjct: 337 HIDNMKILTALVHPKPHITPLQDGLTKRKVHLDVLRRKTVLLLISDLNILQDELSIFEQI 396

Query: 384 FKESQQ-------RPEIRYEIVWIPIID--HSIEQHNKNQHKFKQLQQLMPWFSVHDPSI 443
           + ES++       +  + YE+VW+P++D     E+    Q KF+ L+  MPW+SV  P +
Sbjct: 397 YTESRRNLVGVDGKSHMPYEVVWVPVVDPIEDFERSPILQKKFEDLRDPMPWYSVDSPKL 456

Query: 444 IELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFTSEREEALWKT 503
           IE  VV F++ +W+F  K ILV +DPQG  +S NALHM+WIWG  AFPFT  REE LW+ 
Sbjct: 457 IERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRR 516

Query: 504 ESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEAAKVDLQMTYV 563
           E++ L L++DGID  I +W     YI L+GG+D++WI+ FT+  K  A+ + V+L+M YV
Sbjct: 517 ETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNVNLEMAYV 576

Query: 564 GKNN--AKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGKTVENDQIM 623
           GK N   +E++R+I+ +I    LSH W +  L+WFFW RLESM+YSK+  GK  ++D +M
Sbjct: 577 GKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVM 636

Query: 624 QEIMTLLSFDGSDKGWAIFFGRGGEMTR-AKGEIALSCILAFEEWKDEVEEKGFLTALA- 683
           Q I  +LS+D    GWA+   +G E+   A G I  +  +    WK  V  KG+  A++ 
Sbjct: 637 QGIKKILSYDKLG-GWAL-LSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYTKAMSD 696

Query: 684 ----EYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCC 713
               E L++   P       +   +G IPE + C EC R MEKY+ + CC
Sbjct: 697 HHHDEVLRETGKPCGHFDFHITARSGRIPEKMNCFECQRPMEKYMSFSCC 727

BLAST of Spg007596 vs. ExPASy Swiss-Prot
Match: Q93XX2 (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 3.6e-129
Identity = 267/727 (36.73%), Postives = 393/727 (54.06%), Query Frame = 0

Query: 16  KNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQH 75
           +N + M S +DD  M  +VL TH+PD     V  +L  V DI +   P I    D +   
Sbjct: 128 RNGRPMFSLSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIFKSHVPSI----DSSAPK 187

Query: 76  EDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC-SGGDAH-------- 135
             ++    +        E+ A +I ++  E++          CKC  GG++H        
Sbjct: 188 PSLVFKDYAD---HTSFETFADLIDQISCEID----------CKCLHGGESHGMMTSGLH 247

Query: 136 -----ATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQL 195
                 TT ++L+L+S Y WDAK+V+ ++A AV YG + LLA+ + TN L K+LAL+KQL
Sbjct: 248 LDSRNTTTFSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQL 307

Query: 196 PDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAA 255
           P +    N+L  + D    L++ M+D+T  I++  +LP  +I+      +    H PTA 
Sbjct: 308 PSIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHIT------AAFTDHIPTAV 367

Query: 256 YWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHID 315
           YW ++  + C S I        ++IMS  EV E+   + +   I  +L  Q       I+
Sbjct: 368 YWIVRCVLICVSHISGASGFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMTIE 427

Query: 316 GKRHLEAYQNLVRISET-LHLDNMKLIRALISTRDDIQPLYD--GTSKTTVHLEILKRKH 375
                E YQ L++   T +H+D   ++  L+     I  LY   G SK  V + +L +KH
Sbjct: 428 EGIIEEEYQELIQTFTTIIHVD---VVPPLLRLLRPIDFLYHGAGVSKRRVGINVLTQKH 487

Query: 376 VLLLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQ 435
           VLLLISDL+   +E+ IL++L+ E+ Q+    +EI+W+P+ D   E    +  KF+ L  
Sbjct: 488 VLLLISDLENIEKELYILESLYTEAWQQ---SFEILWVPVQDFWTE---ADDAKFEALHM 547

Query: 436 LMPWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFP 495
            M W+ + +P  +  + +RF++E W F  + ILVALDP+G+V STNA  M+WIW   A P
Sbjct: 548 NMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVWIWQPFAHP 607

Query: 496 FTSEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVA 555
           FT+ RE  LW  + W LE LIDG D   L+   +G+YICL+GGEDM+WIK+FT   + VA
Sbjct: 608 FTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVDGKYICLYGGEDMQWIKNFTSLWRNVA 667

Query: 556 EAAKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLN- 615
           +AA + L+M YVGK N K  ++ I   I +  LSH  PD   +WFFW R+ESM  SK   
Sbjct: 668 KAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVESMWESKQRM 727

Query: 616 ---YG-------KTVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCIL 675
              +G       K  E D ++QE++ +L + G   GW +       M RAKG +    + 
Sbjct: 728 LKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAKGNLFSRGLA 787

Query: 676 AFEEWKDEVEEKGFLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYL 715
            F EW+  +  KGFLTAL ++L     PHHC R +LP  AG IP  V C EC R MEKY 
Sbjct: 788 EFNEWEVNIPTKGFLTALNDHLLMRLPPHHCTRFMLPETAGIIPNEVECTECRRTMEKYY 822

BLAST of Spg007596 vs. ExPASy Swiss-Prot
Match: Q9FXE2 (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 SV=2)

HSP 1 Score: 270.4 bits (690), Expect = 5.8e-71
Identity = 199/709 (28.07%), Postives = 340/709 (47.95%), Query Frame = 0

Query: 17  NDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQHE 76
           N +R +SA +++ + +Q+L +H PDGR +    +L  VE I       +  VL  +    
Sbjct: 2   NFRRDISALNEDIIVEQLLRSHDPDGRWLDSEMLLQEVETI-------LSFVLQNDVSRP 61

Query: 77  DMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKCSG-GDAHATTMAILNL 136
            + E+ I++ E+    E+L + I ++          S+++ C C+G  +    TM + +L
Sbjct: 62  LLTENCITTIEVFDSKETLPYAIFRI----------SVQMLCPCTGENEIRKRTMVLFDL 121

Query: 137 LSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQLPDVIEHSNSLKPQF 196
           L  Y WDAK V+ +   A  YG   L   L   + +A ++A L QLP  IE +   +P  
Sbjct: 122 LKEYRWDAKAVLVLGVLAATYGGLLLPVHLAICDPVAASIAKLNQLP--IERT-KFRPWL 181

Query: 197 DALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAAYWTIKSAVACTSLI 256
           ++LN LIKAM+DVTKCI++F ++P +    D   +   +++     Y  +KSA+ C   I
Sbjct: 182 ESLNLLIKAMVDVTKCIIKFEKIPFKQAKLDNNILGETLSNIYLTTYRVVKSALTCMQQI 241

Query: 257 ----------ESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHIDGKRH 316
                     +S  + +   I S     ELSSL ++  NI+  L  Q++ C   I+    
Sbjct: 242 PYFKQTQQAKKSRKTAAELSIESRRAAGELSSLGYQLLNIHTRLNKQVEDCSTQIEE--- 301

Query: 317 LEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVLLLISD 376
            E  Q L  I+   H DN  ++  L S +DD+ PL   + +  + +  ++ K  LLL+S 
Sbjct: 302 -EINQRLRNINIETHQDNQDVLHLLFSLQDDL-PLQQYSRQ--ISITEVQDKVTLLLLSK 361

Query: 377 LDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLMPWFSV 436
             +     ++       S    E  YEI+W+P I  S +  ++ +  F      +PW SV
Sbjct: 362 PPVEPLFFLLQQLYDHPSNTNTEQNYEIIWVP-IPSSQKWTDEEKEIFDFYSNSLPWISV 421

Query: 437 HDPSIIELSVVRFIKEKWNF-TKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFTSERE 496
             P ++  +++ F K++W++   + +LV +D  GR  + NA+ M+ IWG  A+PF+  RE
Sbjct: 422 RQPWLMSSTILNFFKQEWHYKDNEAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSRE 481

Query: 497 EALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEAAKVD 556
           + LWK   W + LL+DGI  +      EGR IC+FG E+++WI +F    +++       
Sbjct: 482 DELWKEHGWSINLLLDGIHPTF-----EGREICIFGSENLDWIDEFVSLARKIQNLG-FQ 541

Query: 557 LQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLN--YGKTV 616
           L++ Y+      ER  + + ++            TL   FW RLES+  SKL     +  
Sbjct: 542 LELIYLSNQRRDERAMEESSILF---------SPTLQQLFWLRLESIERSKLKRIVIEPS 601

Query: 617 ENDQIMQEIMTLLSFD-GSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKGF 676
           + D++ +E+  LL FD G  +GW I  G G       GE     +     W +  +  GF
Sbjct: 602 KPDRVFEEVRNLLDFDYGKHRGWGI-IGNGSTAETVDGEKMTERMRKIVRWGEYAKGLGF 661

Query: 677 LTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYR 711
             A+    ++     H    ++P       + V C +C   M++++ Y+
Sbjct: 662 TEAIEIAAEKPCELSH--TAVVPFEEALTMKVVTCEKCKWPMKRFVAYQ 664

BLAST of Spg007596 vs. ExPASy TrEMBL
Match: A0A6J1KN22 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111496075 PE=4 SV=1)

HSP 1 Score: 1298.5 bits (3359), Expect = 0.0e+00
Identity = 640/714 (89.64%), Postives = 676/714 (94.68%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MA+ TRPAP M++LIKND+RMLSA+DDNAMTKQ+LATHAPDGR V  +PIL T+ED+IRR
Sbjct: 1   MASHTRPAPNMHKLIKNDRRMLSASDDNAMTKQILATHAPDGRMVDFKPILFTIEDVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATP IG VLDGNGQHEDMLED ISSAEM+G+LESL +VIQKVG E          LACKC
Sbjct: 61  ATPAIGNVLDGNGQHEDMLEDHISSAEMDGVLESLTYVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHA+TMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHASTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDV+E+S+SLKPQF+ALNKL+KAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA
Sbjct: 181 LPDVMENSSSLKPQFEALNKLVKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
            YWTIKSAVACTSLIESLVSL+HERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI
Sbjct: 241 TYWTIKSAVACTSLIESLVSLTHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           DGK+HLEAYQNLVRISET HLDNMK IRALIS RDDIQPLYDGTSKTTVHLEILKRKHVL
Sbjct: 301 DGKKHLEAYQNLVRISETHHLDNMKFIRALISARDDIQPLYDGTSKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI+HEEVM+LDNLFKESQQR EIRYEIVWIPIID SIEQ  KN+H FK++Q +M
Sbjct: 361 LLISDLDISHEEVMVLDNLFKESQQRQEIRYEIVWIPIIDQSIEQQGKNKHTFKEVQMMM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSVH+PSIIE SV+RFIKEKWNFTKKTILVALDPQG+VSSTNALHMLWIWGN AFPFT
Sbjct: 421 PWFSVHEPSIIERSVIRFIKEKWNFTKKTILVALDPQGKVSSTNALHMLWIWGNQAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIK+FT+KTKQVAE 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKEFTMKTKQVAEE 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           AKVDLQM YVGKNNAKERVRKITIMIG+NKLSHYWPDSTL+WFFWARLESMMYSKLN+G+
Sbjct: 541 AKVDLQMAYVGKNNAKERVRKITIMIGENKLSHYWPDSTLIWFFWARLESMMYSKLNHGR 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGE AL CILA+EEWK EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGETALGCILAYEEWKTEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 704

BLAST of Spg007596 vs. ExPASy TrEMBL
Match: A0A6J1EHL3 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111433459 PE=4 SV=1)

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 638/714 (89.36%), Postives = 671/714 (93.98%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MA+ TRP P M++LIKND+RMLSA+DDNAMTKQ+LATHAPDGR V  +PIL T+ED+IRR
Sbjct: 1   MASHTRPPPNMHKLIKNDRRMLSASDDNAMTKQILATHAPDGRMVDFKPILFTIEDVIRR 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATP IG VLDGNGQHEDMLED I S EM+G LESL +VIQKVG E          LACKC
Sbjct: 61  ATPAIGNVLDGNGQHEDMLEDHIGSPEMDGALESLTYVIQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHA+TMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHASTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDV+E+S SLKPQF+ALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA
Sbjct: 181 LPDVMENSTSLKPQFEALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
           AYWTIKSAVACTSLIESLVSL+HERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI
Sbjct: 241 AYWTIKSAVACTSLIESLVSLTHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           DGK+HLEAYQNLVRISET HLDNMK IRALIS RDDIQPLYDGTSKTTVHLEILKRKHVL
Sbjct: 301 DGKKHLEAYQNLVRISETHHLDNMKFIRALISARDDIQPLYDGTSKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISD DI+HEEVM+LDNLFKESQQR EIRYEIVWIPIID SIEQ  KN+H FK++Q +M
Sbjct: 361 LLISDRDISHEEVMVLDNLFKESQQRQEIRYEIVWIPIIDQSIEQQGKNKHTFKEVQMMM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSVH+PSIIE SV+RFIKEKWNFTKKTILVALDPQG+VSSTNALHMLWIWGN AFPFT
Sbjct: 421 PWFSVHEPSIIERSVIRFIKEKWNFTKKTILVALDPQGKVSSTNALHMLWIWGNQAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIK+FT+KTKQVAE 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKEFTMKTKQVAEE 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           AKVDLQM YVGKNNAKERVRKITIMIG+NKLSHYWPDSTL+WFFWARLESMMYSKLN+G+
Sbjct: 541 AKVDLQMAYVGKNNAKERVRKITIMIGENKLSHYWPDSTLIWFFWARLESMMYSKLNHGR 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGE AL CILA+EEWK EVEEKG
Sbjct: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGETALGCILAYEEWKTEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 704

BLAST of Spg007596 vs. ExPASy TrEMBL
Match: A0A1S3CM32 (protein SIEVE ELEMENT OCCLUSION B-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502357 PE=4 SV=1)

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 599/715 (83.78%), Postives = 650/715 (90.91%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MAA  R APKM+Q  K D+RML A+DDNAMTKQ+LATH+PD  +V V+PILL VE++IR 
Sbjct: 1   MAAFGRLAPKMHQ-TKTDRRMLYASDDNAMTKQILATHSPDSHKVDVKPILLIVEEVIRH 60

Query: 61  ATPD-IGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACK 120
           ATPD IGKV+DGNGQ    L+DQ+S AEM+GMLE LAHV+QKVG E          LACK
Sbjct: 61  ATPDIIGKVIDGNGQ----LDDQLSLAEMDGMLEPLAHVVQKVGAE----------LACK 120

Query: 121 CSGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLK 180
             GGDAHATTMAILNLLSNYSWDAKVVIT+AAFAV YGQYWLLAQLYTTNMLAKALALLK
Sbjct: 121 FLGGDAHATTMAILNLLSNYSWDAKVVITLAAFAVTYGQYWLLAQLYTTNMLAKALALLK 180

Query: 181 QLPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPT 240
           QLPDVIEHSNSLKP FDAL+KLI A+L+VTKCIV+FTELPS YIS DTPAMSVA+A+FPT
Sbjct: 181 QLPDVIEHSNSLKPHFDALSKLIAAILNVTKCIVKFTELPSHYISSDTPAMSVALANFPT 240

Query: 241 AAYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQH 300
           AAYWTIKS VACTSLIESLVSLSHE IMSTTEVWELSSLAHK  NI+EHL+ QL+LC+Q+
Sbjct: 241 AAYWTIKSLVACTSLIESLVSLSHELIMSTTEVWELSSLAHKVKNIHEHLQMQLELCIQY 300

Query: 301 IDGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHV 360
           ID KRH EAYQNLVRISETLHLDNMK IRALIS+R+DI PLYDGT+KTTVHLEILKRKHV
Sbjct: 301 IDEKRHEEAYQNLVRISETLHLDNMKFIRALISSREDIHPLYDGTTKTTVHLEILKRKHV 360

Query: 361 LLLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQL 420
           LLLISDLDI HEEVMILDNLFKES QRPEIRYEIVWIPIID ++EQH+K++HKF++L+QL
Sbjct: 361 LLLISDLDIPHEEVMILDNLFKESHQRPEIRYEIVWIPIIDPAVEQHSKSKHKFEELKQL 420

Query: 421 MPWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPF 480
           MPWFSV+DPSIIELS +RFIKEKWNF KKTILVALDPQG+VSSTNALHMLWIWGNLAFPF
Sbjct: 421 MPWFSVYDPSIIELSTIRFIKEKWNFRKKTILVALDPQGKVSSTNALHMLWIWGNLAFPF 480

Query: 481 TSEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAE 540
           TSEREEALWKTESWRLELLIDGIDLSILDWAAEGRYIC++GGED EWIK+FT KTK+VAE
Sbjct: 481 TSEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICIYGGEDTEWIKEFTSKTKKVAE 540

Query: 541 AAKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYG 600
            A VDLQM YVGKNNAKERVRKI+IMI DNKLSHYWPDSTLVWFFW RLESMMYSKLNYG
Sbjct: 541 TANVDLQMAYVGKNNAKERVRKISIMISDNKLSHYWPDSTLVWFFWTRLESMMYSKLNYG 600

Query: 601 KTVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEK 660
           KTVEND IMQEIMTLLSFDGSDKGWAIFFGR GE TRAKGE  LSC+LAF++WK+EVEEK
Sbjct: 601 KTVENDPIMQEIMTLLSFDGSDKGWAIFFGRAGETTRAKGETVLSCLLAFDQWKEEVEEK 660

Query: 661 GFLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           GF+ AL EYLQQL+TPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 GFVKALDEYLQQLKTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 700

BLAST of Spg007596 vs. ExPASy TrEMBL
Match: A0A1S3CN48 (protein SIEVE ELEMENT OCCLUSION B-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502357 PE=4 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 595/714 (83.33%), Postives = 646/714 (90.48%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MAA  R APKM+Q  K D+RML A+DDNAMTKQ+LATH+PD  +V V+PILL VE++IR 
Sbjct: 1   MAAFGRLAPKMHQ-TKTDRRMLYASDDNAMTKQILATHSPDSHKVDVKPILLIVEEVIRH 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATPDI  +  GNGQ    L+DQ+S AEM+GMLE LAHV+QKVG E          LACK 
Sbjct: 61  ATPDI--IGKGNGQ----LDDQLSLAEMDGMLEPLAHVVQKVGAE----------LACKF 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
            GGDAHATTMAILNLLSNYSWDAKVVIT+AAFAV YGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 LGGDAHATTMAILNLLSNYSWDAKVVITLAAFAVTYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDVIEHSNSLKP FDAL+KLI A+L+VTKCIV+FTELPS YIS DTPAMSVA+A+FPTA
Sbjct: 181 LPDVIEHSNSLKPHFDALSKLIAAILNVTKCIVKFTELPSHYISSDTPAMSVALANFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
           AYWTIKS VACTSLIESLVSLSHE IMSTTEVWELSSLAHK  NI+EHL+ QL+LC+Q+I
Sbjct: 241 AYWTIKSLVACTSLIESLVSLSHELIMSTTEVWELSSLAHKVKNIHEHLQMQLELCIQYI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           D KRH EAYQNLVRISETLHLDNMK IRALIS+R+DI PLYDGT+KTTVHLEILKRKHVL
Sbjct: 301 DEKRHEEAYQNLVRISETLHLDNMKFIRALISSREDIHPLYDGTTKTTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI HEEVMILDNLFKES QRPEIRYEIVWIPIID ++EQH+K++HKF++L+QLM
Sbjct: 361 LLISDLDIPHEEVMILDNLFKESHQRPEIRYEIVWIPIIDPAVEQHSKSKHKFEELKQLM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSV+DPSIIELS +RFIKEKWNF KKTILVALDPQG+VSSTNALHMLWIWGNLAFPFT
Sbjct: 421 PWFSVYDPSIIELSTIRFIKEKWNFRKKTILVALDPQGKVSSTNALHMLWIWGNLAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYIC++GGED EWIK+FT KTK+VAE 
Sbjct: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICIYGGEDTEWIKEFTSKTKKVAET 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           A VDLQM YVGKNNAKERVRKI+IMI DNKLSHYWPDSTLVWFFW RLESMMYSKLNYGK
Sbjct: 541 ANVDLQMAYVGKNNAKERVRKISIMISDNKLSHYWPDSTLVWFFWTRLESMMYSKLNYGK 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVEND IMQEIMTLLSFDGSDKGWAIFFGR GE TRAKGE  LSC+LAF++WK+EVEEKG
Sbjct: 601 TVENDPIMQEIMTLLSFDGSDKGWAIFFGRAGETTRAKGETVLSCLLAFDQWKEEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           F+ AL EYLQQL+TPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FVKALDEYLQQLKTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 697

BLAST of Spg007596 vs. ExPASy TrEMBL
Match: A0A0A0LP10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002870 PE=4 SV=1)

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 590/714 (82.63%), Postives = 642/714 (89.92%), Query Frame = 0

Query: 1   MAALTRPAPKMNQLIKNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRR 60
           MAA  RPA KM+QL K D+RML  +DDNAMTKQ+LATH+PD  +V V+PILL VE++IR 
Sbjct: 1   MAAFGRPALKMHQLTKTDRRMLPVSDDNAMTKQILATHSPDSHKVDVKPILLIVEEVIRH 60

Query: 61  ATPDIGKVLDGNGQHEDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC 120
           ATPDI  +  GNGQ    L+DQ+  AEM+GMLE LAHV+QKVG E          LACKC
Sbjct: 61  ATPDI--IAKGNGQ----LDDQLGLAEMDGMLEPLAHVVQKVGAE----------LACKC 120

Query: 121 SGGDAHATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQ 180
           SGGDAHATTMAILNLLSNYSWDAKVVIT+AAF+V YGQYWLLAQLYTTNMLAKALALLKQ
Sbjct: 121 SGGDAHATTMAILNLLSNYSWDAKVVITLAAFSVTYGQYWLLAQLYTTNMLAKALALLKQ 180

Query: 181 LPDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTA 240
           LPDVIEHSNSLKP FDAL+KLI A+L+VTKCIV+FTELPSQYIS DTPAMSVA+A FPTA
Sbjct: 181 LPDVIEHSNSLKPHFDALSKLIAAILNVTKCIVKFTELPSQYISSDTPAMSVALASFPTA 240

Query: 241 AYWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHI 300
           AYWTIKS VACTSLIESLVSLSHE IMSTTEVWELSSLAHK  +I+ HL+ QL LC+Q+I
Sbjct: 241 AYWTIKSLVACTSLIESLVSLSHELIMSTTEVWELSSLAHKVKDIHGHLQMQLKLCIQYI 300

Query: 301 DGKRHLEAYQNLVRISETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVL 360
           D KRH EAYQNLVRISETLHLDNMK IRA ISTR+DI P+YDGT+K TVHLEILKRKHVL
Sbjct: 301 DEKRHEEAYQNLVRISETLHLDNMKFIRAFISTREDIHPIYDGTTKMTVHLEILKRKHVL 360

Query: 361 LLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLM 420
           LLISDLDI HEEVMILDNLFKE+ QRPEIRYEIVWIPIID +IEQH+K++HKF++L+QLM
Sbjct: 361 LLISDLDIPHEEVMILDNLFKEAHQRPEIRYEIVWIPIIDPAIEQHSKSKHKFEELKQLM 420

Query: 421 PWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFT 480
           PWFSV+DPSIIELS +RFIKEKWNF KKTILVALDPQG+VSSTNALHM+WIWGNLAFPFT
Sbjct: 421 PWFSVYDPSIIELSTIRFIKEKWNFRKKTILVALDPQGKVSSTNALHMIWIWGNLAFPFT 480

Query: 481 SEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEA 540
           SEREE LWKTESWRLELLIDGID SILDWAAEGRYIC++GGED EWIK+FT KTK+VAE 
Sbjct: 481 SEREEELWKTESWRLELLIDGIDFSILDWAAEGRYICIYGGEDTEWIKEFTSKTKKVAET 540

Query: 541 AKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGK 600
           A VDLQM YVGKNNAKERVRKI+IMI DNKLSHYW DSTLVWFFWARLESMMYSKLNYGK
Sbjct: 541 ANVDLQMAYVGKNNAKERVRKISIMISDNKLSHYWADSTLVWFFWARLESMMYSKLNYGK 600

Query: 601 TVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKG 660
           TVEND IMQEIMTLLSFDGSDKGWAIFFGR GE TRAKGE  LSCILAF++WK+EVEEKG
Sbjct: 601 TVENDPIMQEIMTLLSFDGSDKGWAIFFGRAGETTRAKGETVLSCILAFDQWKEEVEEKG 660

Query: 661 FLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 715
           F+ ALA+YLQQL+TPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE
Sbjct: 661 FVKALADYLQQLKTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCCVE 698

BLAST of Spg007596 vs. TAIR 10
Match: AT3G01680.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01670.1); Has 122 Blast hits to 112 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 558.1 bits (1437), Expect = 9.9e-159
Identity = 291/710 (40.99%), Postives = 440/710 (61.97%), Query Frame = 0

Query: 24  ATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQHEDML---- 83
           ++D++ M K +  TH+PD REV VR +L  VEDI+ RAT      LD    +  ML    
Sbjct: 37  SSDESMMLKLIQQTHSPDAREVQVRGLLSLVEDILDRAT------LDSEDTNASMLPLPT 96

Query: 84  EDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKCSGGDAHATTMAILNLLSNY 143
           ED++  + M  +L+S+++ I +V  E+ Y  +         +G D+H  TM++   LS++
Sbjct: 97  EDKLMQSSMMSVLDSVSYAIDRVACEIAYKSL---------TGSDSHEITMSVFEHLSSF 156

Query: 144 SWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQLPDVIEHSNSLKPQFDALN 203
            WD K+V+T+AAFA+NYG++WLL Q Y+ N LAK+LA+LK +P  +++  +L+     LN
Sbjct: 157 QWDGKLVLTLAAFALNYGEFWLLVQFYSKNQLAKSLAMLKLVP--VQNRVTLESVSQGLN 216

Query: 204 KLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAAYWTIKSAVACTSLIESLV 263
            LI+ M  VT C+VE +ELP +YI+ D P +S  ++  P A YWTI+S +AC S I  + 
Sbjct: 217 DLIREMKSVTACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMIT 276

Query: 264 SLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHIDGKRHLEAYQNLVRISETL 323
           ++ HE + +  ++WE S LA+K  NI++HL   L LC +HI+ +R  E+ + L  + +T 
Sbjct: 277 AMGHEMMNTQMDLWETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTT 336

Query: 324 HLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVLLLISDLDITHEEVMILDNL 383
           H+DNMK++ AL+  +  I PL DG +K  VHL++L+RK VLLLISDL+I  +E+ I + +
Sbjct: 337 HIDNMKILTALVHPKPHITPLQDGLTKRKVHLDVLRRKTVLLLISDLNILQDELSIFEQI 396

Query: 384 FKESQQ-------RPEIRYEIVWIPIID--HSIEQHNKNQHKFKQLQQLMPWFSVHDPSI 443
           + ES++       +  + YE+VW+P++D     E+    Q KF+ L+  MPW+SV  P +
Sbjct: 397 YTESRRNLVGVDGKSHMPYEVVWVPVVDPIEDFERSPILQKKFEDLRDPMPWYSVDSPKL 456

Query: 444 IELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFTSEREEALWKT 503
           IE  VV F++ +W+F  K ILV +DPQG  +S NALHM+WIWG  AFPFT  REE LW+ 
Sbjct: 457 IERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRR 516

Query: 504 ESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEAAKVDLQMTYV 563
           E++ L L++DGID  I +W     YI L+GG+D++WI+ FT+  K  A+ + V+L+M YV
Sbjct: 517 ETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNVNLEMAYV 576

Query: 564 GKNN--AKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLNYGKTVENDQIM 623
           GK N   +E++R+I+ +I    LSH W +  L+WFFW RLESM+YSK+  GK  ++D +M
Sbjct: 577 GKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVM 636

Query: 624 QEIMTLLSFDGSDKGWAIFFGRGGEMTR-AKGEIALSCILAFEEWKDEVEEKGFLTALA- 683
           Q I  +LS+D    GWA+   +G E+   A G I  +  +    WK  V  KG+  A++ 
Sbjct: 637 QGIKKILSYDKLG-GWAL-LSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYTKAMSD 696

Query: 684 ----EYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYRCC 713
               E L++   P       +   +G IPE + C EC R MEKY+ + CC
Sbjct: 697 HHHDEVLRETGKPCGHFDFHITARSGRIPEKMNCFECQRPMEKYMSFSCC 727

BLAST of Spg007596 vs. TAIR 10
Match: AT3G01670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 121 Blast hits to 111 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 121; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 463.8 bits (1192), Expect = 2.6e-130
Identity = 267/727 (36.73%), Postives = 393/727 (54.06%), Query Frame = 0

Query: 16  KNDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQH 75
           +N + M S +DD  M  +VL TH+PD     V  +L  V DI +   P I    D +   
Sbjct: 128 RNGRPMFSLSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIFKSHVPSI----DSSAPK 187

Query: 76  EDMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKC-SGGDAH-------- 135
             ++    +        E+ A +I ++  E++          CKC  GG++H        
Sbjct: 188 PSLVFKDYAD---HTSFETFADLIDQISCEID----------CKCLHGGESHGMMTSGLH 247

Query: 136 -----ATTMAILNLLSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQL 195
                 TT ++L+L+S Y WDAK+V+ ++A AV YG + LLA+ + TN L K+LAL+KQL
Sbjct: 248 LDSRNTTTFSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQL 307

Query: 196 PDVIEHSNSLKPQFDALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAA 255
           P +    N+L  + D    L++ M+D+T  I++  +LP  +I+      +    H PTA 
Sbjct: 308 PSIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHIT------AAFTDHIPTAV 367

Query: 256 YWTIKSAVACTSLIESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHID 315
           YW ++  + C S I        ++IMS  EV E+   + +   I  +L  Q       I+
Sbjct: 368 YWIVRCVLICVSHISGASGFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMTIE 427

Query: 316 GKRHLEAYQNLVRISET-LHLDNMKLIRALISTRDDIQPLYD--GTSKTTVHLEILKRKH 375
                E YQ L++   T +H+D   ++  L+     I  LY   G SK  V + +L +KH
Sbjct: 428 EGIIEEEYQELIQTFTTIIHVD---VVPPLLRLLRPIDFLYHGAGVSKRRVGINVLTQKH 487

Query: 376 VLLLISDLDITHEEVMILDNLFKESQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQ 435
           VLLLISDL+   +E+ IL++L+ E+ Q+    +EI+W+P+ D   E    +  KF+ L  
Sbjct: 488 VLLLISDLENIEKELYILESLYTEAWQQ---SFEILWVPVQDFWTE---ADDAKFEALHM 547

Query: 436 LMPWFSVHDPSIIELSVVRFIKEKWNFTKKTILVALDPQGRVSSTNALHMLWIWGNLAFP 495
            M W+ + +P  +  + +RF++E W F  + ILVALDP+G+V STNA  M+WIW   A P
Sbjct: 548 NMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVWIWQPFAHP 607

Query: 496 FTSEREEALWKTESWRLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVA 555
           FT+ RE  LW  + W LE LIDG D   L+   +G+YICL+GGEDM+WIK+FT   + VA
Sbjct: 608 FTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVDGKYICLYGGEDMQWIKNFTSLWRNVA 667

Query: 556 EAAKVDLQMTYVGKNNAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLN- 615
           +AA + L+M YVGK N K  ++ I   I +  LSH  PD   +WFFW R+ESM  SK   
Sbjct: 668 KAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVESMWESKQRM 727

Query: 616 ---YG-------KTVENDQIMQEIMTLLSFDGSDKGWAIFFGRGGEMTRAKGEIALSCIL 675
              +G       K  E D ++QE++ +L + G   GW +       M RAKG +    + 
Sbjct: 728 LKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAKGNLFSRGLA 787

Query: 676 AFEEWKDEVEEKGFLTALAEYLQQLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYL 715
            F EW+  +  KGFLTAL ++L     PHHC R +LP  AG IP  V C EC R MEKY 
Sbjct: 788 EFNEWEVNIPTKGFLTALNDHLLMRLPPHHCTRFMLPETAGIIPNEVECTECRRTMEKYY 822

BLAST of Spg007596 vs. TAIR 10
Match: AT1G67790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 208 Blast hits to 125 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 224.6 bits (571), Expect = 2.6e-58
Identity = 176/700 (25.14%), Postives = 308/700 (44.00%), Query Frame = 0

Query: 17  NDKRMLSATDDNAMTKQVLATHAPDGREVFVRPILLTVEDIIRRATPDIGKVLDGNGQHE 76
           N +R +SA +++ + +Q+L +H PDGR +    +L  VE I       +  VL  +    
Sbjct: 2   NFRRDISALNEDIIVEQLLRSHDPDGRWLDSEMLLQEVETI-------LSFVLQNDVSRP 61

Query: 77  DMLEDQISSAEMEGMLESLAHVIQKVGGEVEYICICSLKLACKCSG-GDAHATTMAILNL 136
            + E+ I++ E+    E+L + I ++          S+++ C C+G  +    TM + +L
Sbjct: 62  LLTENCITTIEVFDSKETLPYAIFRI----------SVQMLCPCTGENEIRKRTMVLFDL 121

Query: 137 LSNYSWDAKVVITMAAFAVNYGQYWLLAQLYTTNMLAKALALLKQLPDVIEHSNSLKPQF 196
           L  Y WDAK V+ +   A  YG   L   L   + +A ++A L QLP  IE +   +P  
Sbjct: 122 LKEYRWDAKAVLVLGVLAATYGGLLLPVHLAICDPVAASIAKLNQLP--IERT-KFRPWL 181

Query: 197 DALNKLIKAMLDVTKCIVEFTELPSQYISQDTPAMSVAIAHFPTAAYWTIKSAVACTSLI 256
           ++LN LIKAM+DVTKCI++F ++P +    D   +   +++     Y  +KSA+ C   +
Sbjct: 182 ESLNLLIKAMVDVTKCIIKFEKIPFKQAKLDNNILGETLSNIYLTTYRVVKSALTC---M 241

Query: 257 ESLVSLSHERIMSTTEVWELSSLAHKETNIYEHLRTQLDLCLQHIDGKRHLEAYQNLVRI 316
           + +      + +S TEV +  +L                                     
Sbjct: 242 QQIPYFKQTQQISITEVQDKVTL------------------------------------- 301

Query: 317 SETLHLDNMKLIRALISTRDDIQPLYDGTSKTTVHLEILKRKHVLLLISDLDITHEEVMI 376
                         L+ ++  ++PL+                                 +
Sbjct: 302 --------------LLLSKPPVEPLF--------------------------------FL 361

Query: 377 LDNLFKE-SQQRPEIRYEIVWIPIIDHSIEQHNKNQHKFKQLQQLMPWFSVHDPSIIELS 436
           L  L+   S    E  YEI+W+P I  S +  ++ +  F      +PW SV  P ++  +
Sbjct: 362 LQQLYDHPSNTNTEQNYEIIWVP-IPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSST 421

Query: 437 VVRFIKEKWNF-TKKTILVALDPQGRVSSTNALHMLWIWGNLAFPFTSEREEALWKTESW 496
           ++ F K++W++   + +LV +D  GR  + NA+ M+ IWG  A+PF+  RE+ LWK   W
Sbjct: 422 ILNFFKQEWHYKDNEAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEHGW 481

Query: 497 RLELLIDGIDLSILDWAAEGRYICLFGGEDMEWIKDFTIKTKQVAEAAKVDLQMTYVGKN 556
            + LL+DGI  +      EGR IC+FG E+++WI +F    +++       L++ Y+   
Sbjct: 482 SINLLLDGIHPTF-----EGREICIFGSENLDWIDEFVSLARKIQNLG-FQLELIYLSNQ 541

Query: 557 NAKERVRKITIMIGDNKLSHYWPDSTLVWFFWARLESMMYSKLN--YGKTVENDQIMQEI 616
              ER  + + ++            TL   FW RLES+  SKL     +  + D++ +E+
Sbjct: 542 RRDERAMEESSILF---------SPTLQQLFWLRLESIERSKLKRIVIEPSKPDRVFEEV 576

Query: 617 MTLLSFD-GSDKGWAIFFGRGGEMTRAKGEIALSCILAFEEWKDEVEEKGFLTALAEYLQ 676
             LL FD G  +GW I  G G       GE     +     W +  +  GF  A+    +
Sbjct: 602 RNLLDFDYGKHRGWGI-IGNGSTAETVDGEKMTERMRKIVRWGEYAKGLGFTEAIEIAAE 576

Query: 677 QLRTPHHCNRLILPGLAGNIPENVVCAECGRAMEKYLMYR 711
           +     H    ++P       + V C +C   M++++ Y+
Sbjct: 662 KPCELSH--TAVVPFEEALTMKVVTCEKCKWPMKRFVAYQ 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023002120.10.0e+0089.64protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita maxima][more]
XP_023530744.10.0e+0089.22protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo][more]
KAG6587435.10.0e+0089.36Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022926283.10.0e+0089.36protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita moschata][more]
XP_038880148.10.0e+0088.66protein SIEVE ELEMENT OCCLUSION B-like isoform X3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SS871.4e-15740.99Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 S... [more]
Q93XX23.6e-12936.73Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 S... [more]
Q9FXE25.8e-7128.07Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 S... [more]
Match NameE-valueIdentityDescription
A0A6J1KN220.0e+0089.64protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC1114960... [more]
A0A6J1EHL30.0e+0089.36protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A1S3CM320.0e+0083.78protein SIEVE ELEMENT OCCLUSION B-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S3CN480.0e+0083.33protein SIEVE ELEMENT OCCLUSION B-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0LP100.0e+0082.63Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002870 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G01680.19.9e-15940.99CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640);... [more]
AT3G01670.12.6e-13036.73unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G67790.12.6e-5825.14unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027942Sieve element occlusion, N-terminalPFAMPF14576SEO_Ncoord: 26..320
e-value: 1.1E-115
score: 385.8
IPR027944Sieve element occlusion, C-terminalPFAMPF14577SEO_Ccoord: 482..712
e-value: 4.6E-100
score: 333.8
NoneNo IPR availablePANTHERPTHR33232:SF20PROTEIN SIEVE ELEMENT OCCLUSION B-LIKEcoord: 8..713
IPR039299Protein SIEVE ELEMENT OCCLUSIONPANTHERPTHR33232PROTEIN SIEVE ELEMENT OCCLUSION B-LIKEcoord: 8..713

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg007596.1Spg007596.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010088 phloem development