Spg007590 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg007590
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450 71A25-like
Location	scaffold2: 862055 .. 863517 (-)
RNA-Seq Expression	Spg007590
Synteny	Spg007590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTGTGATCTTCTTCTCCATTTGTTCTTTCATCCAATTTCCCTCACTGCTTTGATCGTCTTCCTCATCTTCCTTCTCCAAAAATGGTTGAGCTCTTCATCCTCAGAAGCCTCGCCCCCTTCTCCGCCAAAGAAGCTTCCCATTTTCGGACACCTTTTGAGCTTCGGCTCCCATCCCCATCATCTCACTTTCCAAGCCTATGCCCGCCGCTACGGCCCTCTGTTTCTGCTTCACCTCGGCTCCAAGCCCACCATCGTCGTCTCTTCAGCAGAATTGGCTCGCGAAATCATGAAGACCCACGATCTCGTCTTTTCCAACCGACCCAAATCAATCAAATCTGATAAGCTTCTTTACGGCTCCAAAGATATAACCGCAGCTCCTTACGGCGAGTACTGGAGGCAGATGAAAAGCCTGGCCGTGCTCCATCTTTTGAGCAACAAAAGAGTTCGATCCTTTCGCTTTGTCAGAGAAGAAGAAGTCAAATTGATGATCGAGAACATCCAACGAAACCCAGCGTCCGTGAATTTGACAGAATTGTTCTCTCAACTGACCAACAACGTCATTTGCAGGGTGGCTTTAGGGAGAAAATATGGGGTGGGAGAAGATGGGAAGAATCTCCACTCTGTTTTGCTGGAGGGGATGAAATTATTGGGGAGTTTTAGTATCAGAAACTTCATTCCATGGCTGGGCTGGATCGATGCTATCACTGGACTGGATGCCAAAATCGAGAGGGTTGCCAAAGAGTTGGATGCTTTCTTAGACAGAGTGATTGAAGATCATGTCAATCCAGAAAATCGTGACGAGCACAAAGACTTGGTGGACATTCTACTCTGTATACAGAGAGAGAAATCCATGGGTTTTCCCCTGAACATGGATAGCATAAAAGCTTTACTCTTGGTAATGGGTGATCCCTAAATGTAATCAATCTCATTTGTAAGTTAAGAATAATCTATGATTCGTTTTGATTACAGGACATGTTTGTGGCAGGCAAGGACACGACTTACACATTAATGGAGTGGACAATGTCAGAATTGTTGAGACACCCAGATGCCATGAAGAAATTGCAGAGTGAGATCAGAGAAATTACAGGTGACAGAGCATACGTAGACGATGATGATTTGGAAAAGATGCATTACTTGAAAGCAGTAATCAAAGAAACGTTGCGACTACACCCACCAGTCCCACTTCTTTCTCCTAGAGAACCGACTCAATACGTCAAGCTAAAAGGCTACGATATCAAACCGGGCACTCGAGTTACGATCAATGCATGGGCCATCGGAAGAGACCCGAAACTATGGGAAGAAGCAGAGGAGTTTCGGCCGGAAAGGTTTATGAATAGATGGATGGATTTCAAGGGTCATGATTTCGAGCTAGTCCCGTTTGGTGCAGGGAGAAGAGGATGCCCGGGAGTTGTGTTTGCAATGGTAAATGTTGATGTTAAACTGAAACCTCGAGCTTAG

mRNA sequence

ATGGACTGTGATCTTCTTCTCCATTTGTTCTTTCATCCAATTTCCCTCACTGCTTTGATCGTCTTCCTCATCTTCCTTCTCCAAAAATGGTTGAGCTCTTCATCCTCAGAAGCCTCGCCCCCTTCTCCGCCAAAGAAGCTTCCCATTTTCGGACACCTTTTGAGCTTCGGCTCCCATCCCCATCATCTCACTTTCCAAGCCTATGCCCGCCGCTACGGCCCTCTGTTTCTGCTTCACCTCGGCTCCAAGCCCACCATCGTCGTCTCTTCAGCAGAATTGGCTCGCGAAATCATGAAGACCCACGATCTCGTCTTTTCCAACCGACCCAAATCAATCAAATCTGATAAGCTTCTTTACGGCTCCAAAGATATAACCGCAGCTCCTTACGGCGAGTACTGGAGGCAGATGAAAAGCCTGGCCGTGCTCCATCTTTTGAGCAACAAAAGAGTTCGATCCTTTCGCTTTGTCAGAGAAGAAGAAGTCAAATTGATGATCGAGAACATCCAACGAAACCCAGCGTCCGTGAATTTGACAGAATTGTTCTCTCAACTGACCAACAACGTCATTTGCAGGGTGGCTTTAGGGAGAAAATATGGGGTGGGAGAAGATGGGAAGAATCTCCACTCTGTTTTGCTGGAGGGGATGAAATTATTGGGGAGTTTTAGTATCAGAAACTTCATTCCATGGCTGGGCTGGATCGATGCTATCACTGGACTGGATGCCAAAATCGAGAGGGTTGCCAAAGAGTTGGATGCTTTCTTAGACAGAGTGATTGAAGATCATGTCAATCCAGAAAATCGTGACGAGCACAAAGACTTGGTGGACATTCTACTCTGTATACAGAGAGAGAAATCCATGGGTTTTCCCCTGAACATGGATAGCATAAAAGCTTTACTCTTGGACATGTTTGTGGCAGGCAAGGACACGACTTACACATTAATGGAGTGGACAATGTCAGAATTGTTGAGACACCCAGATGCCATGAAGAAATTGCAGAGTGAGATCAGAGAAATTACAGGTGACAGAGCATACGTAGACGATGATGATTTGGAAAAGATGCATTACTTGAAAGCAGTAATCAAAGAAACGTTGCGACTACACCCACCAGTCCCACTTCTTTCTCCTAGAGAACCGACTCAATACGTCAAGCTAAAAGGCTACGATATCAAACCGGGCACTCGAGTTACGATCAATGCATGGGCCATCGGAAGAGACCCGAAACTATGGGAAGAAGCAGAGGAGTTTCGGCCGGAAAGGTTTATGAATAGATGGATGGATTTCAAGGGTCATGATTTCGAGCTAGTCCCGTTTGGTGCAGGGAGAAGAGGATGCCCGGGAGTTGTGTTTGCAATGGTAAATGTTGATGTTAAACTGAAACCTCGAGCTTAG

Coding sequence (CDS)

ATGGACTGTGATCTTCTTCTCCATTTGTTCTTTCATCCAATTTCCCTCACTGCTTTGATCGTCTTCCTCATCTTCCTTCTCCAAAAATGGTTGAGCTCTTCATCCTCAGAAGCCTCGCCCCCTTCTCCGCCAAAGAAGCTTCCCATTTTCGGACACCTTTTGAGCTTCGGCTCCCATCCCCATCATCTCACTTTCCAAGCCTATGCCCGCCGCTACGGCCCTCTGTTTCTGCTTCACCTCGGCTCCAAGCCCACCATCGTCGTCTCTTCAGCAGAATTGGCTCGCGAAATCATGAAGACCCACGATCTCGTCTTTTCCAACCGACCCAAATCAATCAAATCTGATAAGCTTCTTTACGGCTCCAAAGATATAACCGCAGCTCCTTACGGCGAGTACTGGAGGCAGATGAAAAGCCTGGCCGTGCTCCATCTTTTGAGCAACAAAAGAGTTCGATCCTTTCGCTTTGTCAGAGAAGAAGAAGTCAAATTGATGATCGAGAACATCCAACGAAACCCAGCGTCCGTGAATTTGACAGAATTGTTCTCTCAACTGACCAACAACGTCATTTGCAGGGTGGCTTTAGGGAGAAAATATGGGGTGGGAGAAGATGGGAAGAATCTCCACTCTGTTTTGCTGGAGGGGATGAAATTATTGGGGAGTTTTAGTATCAGAAACTTCATTCCATGGCTGGGCTGGATCGATGCTATCACTGGACTGGATGCCAAAATCGAGAGGGTTGCCAAAGAGTTGGATGCTTTCTTAGACAGAGTGATTGAAGATCATGTCAATCCAGAAAATCGTGACGAGCACAAAGACTTGGTGGACATTCTACTCTGTATACAGAGAGAGAAATCCATGGGTTTTCCCCTGAACATGGATAGCATAAAAGCTTTACTCTTGGACATGTTTGTGGCAGGCAAGGACACGACTTACACATTAATGGAGTGGACAATGTCAGAATTGTTGAGACACCCAGATGCCATGAAGAAATTGCAGAGTGAGATCAGAGAAATTACAGGTGACAGAGCATACGTAGACGATGATGATTTGGAAAAGATGCATTACTTGAAAGCAGTAATCAAAGAAACGTTGCGACTACACCCACCAGTCCCACTTCTTTCTCCTAGAGAACCGACTCAATACGTCAAGCTAAAAGGCTACGATATCAAACCGGGCACTCGAGTTACGATCAATGCATGGGCCATCGGAAGAGACCCGAAACTATGGGAAGAAGCAGAGGAGTTTCGGCCGGAAAGGTTTATGAATAGATGGATGGATTTCAAGGGTCATGATTTCGAGCTAGTCCCGTTTGGTGCAGGGAGAAGAGGATGCCCGGGAGTTGTGTTTGCAATGGTAAATGTTGATGTTAAACTGAAACCTCGAGCTTAG

Protein sequence

MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTELFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDAFLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAEEFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKLKPRA

Homology

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BLAST of Spg007590 vs. NCBI nr
Match: XP_022921784.1 (cytochrome P450 71A3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 691.4 bits (1783), Expect = 5.2e-195
Identity = 343/465 (73.76%), Postives = 397/465 (85.38%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR+IMKTHDLVF+NRPKS  +DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDIMKTHDLVFANRPKSSMADKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED  NL S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDLINLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DA+ +RVAKELD FL RVI+DHV+ ENRDE    HKDLVD+LL IQR  ++GFPL MDSI
Sbjct: 241 DARAKRVAKELDVFLGRVIQDHVDSENRDENSGKHKDLVDVLLWIQRNNTIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG +TT T+MEWTMSELL+HP+AMKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFAAGTETTSTVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KLKGYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKGHDF+L+PFGAGRRGCPG+ FA V  ++ L
Sbjct: 421 EFRPERFMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIAL 464

[Close]

BLAST of Spg007590 vs. NCBI nr
Match: XP_022921787.1 (cytochrome P450 71A3-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 688.7 bits (1776), Expect = 3.4e-194
Identity = 344/463 (74.30%), Postives = 395/463 (85.31%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR+IMKTHDLVF+NRPKS  +DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDIMKTHDLVFANRPKSSMADKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED  NL S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDLINLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DA+ +RVAKELD FL RVI+DHV+ ENRDE    HKDLVD+LL IQR  ++GFPL MDSI
Sbjct: 241 DARAKRVAKELDVFLGRVIQDHVDSENRDENSGKHKDLVDVLLWIQRNNTIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHY 360
           KAL+LDMF AG DT  T++EW MSELL+HP+AMKKL+SEIREI+GD  YV++DDL+KMHY
Sbjct: 301 KALILDMFSAGTDTISTVLEWAMSELLKHPEAMKKLKSEIREISGDH-YVNEDDLDKMHY 360

Query: 361 LKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAEEF 420
           LKAVIKETLRLHPP+PLL PRE TQY+KL+GYDIKP TRV INAWAIGRDP++WEEAEEF
Sbjct: 361 LKAVIKETLRLHPPIPLLVPRESTQYIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEF 420

Query: 421 RPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           RPERFMN  +DFKG DFEL+PFGAGRRGCPGV FA V  ++ L
Sbjct: 421 RPERFMNSSIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGL 461

[Close]

BLAST of Spg007590 vs. NCBI nr
Match: XP_022921783.1 (cytochrome P450 71A22-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 688.0 bits (1774), Expect = 5.8e-194
Identity = 342/465 (73.55%), Postives = 395/465 (84.95%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+ELAR++MKTHDLVF+NRPKS K DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSELARDVMKTHDLVFANRPKSTKHDKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            SK + AAPYGEYWRQMKS+ VLHLLSN+RV+SFR VREEEVKLMIE IQ+NP SVNLTE
Sbjct: 121 NSKVVAAAPYGEYWRQMKSIDVLHLLSNRRVQSFRSVREEEVKLMIEKIQQNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTN+V+CRVALGRK+  GEDG  L S+LLE ++LLGSF IR+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNDVVCRVALGRKFDAGEDGMKLRSLLLELLELLGSFGIRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DAK +RVAKELD FLDRVI+D V+ ENRDE    HKDLVD+LL +QR  S+GFPL MDSI
Sbjct: 241 DAKGKRVAKELDVFLDRVIQDRVHSENRDENSGKHKDLVDVLLWVQRNNSIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG +TT T+MEWTMSELL+HP+AMKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFAAGTETTSTVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KLKGYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKGHDF+L+PFGAGRRGCPG+ FA V  ++ L
Sbjct: 421 EFRPERFMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIAL 464

[Close]

BLAST of Spg007590 vs. NCBI nr
Match: XP_022921785.1 (cytochrome P450 71A3-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 686.8 bits (1771), Expect = 1.3e-193
Identity = 341/465 (73.33%), Postives = 395/465 (84.95%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR+IMKTHDLVF+NRPKS  +DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDIMKTHDLVFANRPKSSMADKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED  NL S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDLINLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DA+ +RVAKELD FL RVI+DHV+ ENRDE    HKDLVD+LL IQR  ++GFPL MDSI
Sbjct: 241 DARAKRVAKELDVFLGRVIQDHVDSENRDENSGKHKDLVDVLLWIQRNNTIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG DT  T++EW MSELL+HP+AMKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFSAGTDTISTVLEWAMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KLKGYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKGHDF+L+PFGAGRRGCPG+ FA V  ++ L
Sbjct: 421 EFRPERFMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIAL 464

[Close]

BLAST of Spg007590 vs. NCBI nr
Match: XP_023515571.1 (cytochrome P450 71A24-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.9 bits (1766), Expect = 4.9e-193
Identity = 337/465 (72.47%), Postives = 394/465 (84.73%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSS-SSEASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP+S+   I+  I+L  +  SSS   E+SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVGLICHPVSVAGFIILFIWLFFRQESSSQEEESSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGS+PTIVVSS+ELAR++MKTHDLVF+NRPKS K DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSRPTIVVSSSELARDVMKTHDLVFANRPKSTKHDKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            SK + AAPYGEYWRQMKS+ VLHLLSN+RV+SFR VREEEVKLMIE IQ+NP SVNLTE
Sbjct: 121 NSKVVAAAPYGEYWRQMKSIDVLHLLSNRRVQSFRSVREEEVKLMIEKIQQNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTN+V+CRVALGRK+  GEDG  L S+LLE + LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNDVVCRVALGRKFDAGEDGMKLRSLLLELLDLLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DAK +RVAKELD FLDRVI+DHV+ ENRDE    HKDLVD+LL +QR  S+GFPL MDSI
Sbjct: 241 DAKGKRVAKELDVFLDRVIQDHVHSENRDENSGKHKDLVDVLLWVQRNNSIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG +TT T++EWTMSELL+HP+ MKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFAAGTETTSTVLEWTMSELLKHPEVMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KL+GYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKG DFEL+PFGAGRRGCPG+ FA V  +V L
Sbjct: 421 EFRPERFMNNSIDFKGQDFELIPFGAGRRGCPGIAFATVINEVAL 464

[Close]

BLAST of Spg007590 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.4e-144
Identity = 257/461 (55.75%), Postives = 344/461 (74.62%), Query Frame = 0


Query: 10  FFHPISLTALIVFLIFLLQKWLSSSSS----EASPPSPPKKLPIFGHLLSFGSHPHHLTF 69
           F  P++ T L +FL+ LL  W SS+ +       PPSPP KLPI G+L   GS+PH  + 
Sbjct: 17  FLQPLAFTLLAIFLV-LLYTWYSSTKTTTQKSTQPPSPP-KLPIIGNLHQIGSYPHR-SL 76

Query: 70  QAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDIT 129
           QA ++R+GPL LLH GS P +VVSSAE AREI+KTHDL FS+RPKS   +KLLY  KD+ 
Sbjct: 77  QALSQRHGPLMLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVA 136

Query: 130 AAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASV-NLTELFSQL 189
           +APYGEYWRQ++S+ VL+LLSN+RVRSFR VREEE K MI NI+ + +SV NL+E+F +L
Sbjct: 137 SAPYGEYWRQVRSICVLNLLSNRRVRSFRSVREEETKSMIRNIKGSSSSVLNLSEMFVRL 196

Query: 190 TNNVICRVALGRKYGVGEDGKN---LHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDA 249
           TN+V+C+VALGRKY  GE G++      +L E   LLG+ +I +++PWL W+  + GL A
Sbjct: 197 TNDVVCKVALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGA 256

Query: 250 KIERVAKELDAFLDRVIEDHVNPENR---DEHKDLVDILLCIQREKSMGFPLNMDSIKAL 309
           K+++VAK+LD F+D V+++H+N  +R   D+ KD +DILL IQ+E S G P++  S+K +
Sbjct: 257 KLDKVAKQLDDFIDTVVQEHMNHSSRSGDDDQKDFLDILLAIQKETSAGIPIDGVSVKGI 316

Query: 310 LLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA-YVDDDDLEKMHYLK 369
           +LDMF AG DTTY+ +EW M+ELLRHP  M KLQ+E+R I G+R   + +DDL +MHYLK
Sbjct: 317 ILDMFAAGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVEMHYLK 376

Query: 370 AVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAEEFRP 429
           AV KETLRLHPP+PLL PR  T+ V++ GY+IK  T+V I+AW IGRDPKL+++ EEF P
Sbjct: 377 AVTKETLRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEP 436

Query: 430 ERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           ERF+N  +D+KG+DFEL+PFGAGRR CPG+ FAM   ++ L
Sbjct: 437 ERFLNNGIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIAL 474

[Close]

BLAST of Spg007590 vs. ExPASy Swiss-Prot
Match: Q9STK9 (Cytochrome P450 71A24 OS=Arabidopsis thaliana OX=3702 GN=CYP71A24 PE=2 SV=3)

HSP 1 Score: 463.4 bits (1191), Expect = 3.0e-129
Identity = 239/448 (53.35%), Postives = 315/448 (70.31%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           I L   I+ +  L  K  S      +PPSPP +LP+  +L   G HPH  +  + + RYG
Sbjct: 8   ILLLCSIILITILFFKKQSRGKKSNAPPSPP-RLPLIRNLHQLGRHPHR-SLCSLSHRYG 67

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LLH GS P +VVSSA+ A++++KTHD VF++RP+S   DK+ Y  +D+  APYGEYW
Sbjct: 68  PLMLLHFGSVPVLVVSSADAAKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYW 127

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQ-RNPASVNLTELFSQLTNNVICRV 193
           RQMKS+ VLHL SNK VRSFR VR+EE+ LMIE I+  +   +NL+E+   LTNNVICRV
Sbjct: 128 RQMKSVCVLHLFSNKMVRSFRDVRQEEISLMIEKIRISSSLRINLSEILVNLTNNVICRV 187

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKYG   D K+L   L    +LLG FS+ +++ WL WID I GLD ++ +++ +LD 
Sbjct: 188 ALGRKYGGKTDFKDLMKRL---TRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDE 247

Query: 254 FLDRVIEDHVNPENRDEHK-DLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTY 313
           FL+RV++DHV   + D HK D VD LL I+REKS+GF ++  SIKA++LD+FV   DTTY
Sbjct: 248 FLERVVQDHV---DGDGHKNDFVDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTY 307

Query: 314 TLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVP 373
           TL+EW M+ELL H + + +LQ E+R +  D++ V +DDL+ M YLKAVIKETLRLHPP+P
Sbjct: 308 TLLEWAMTELLCHHECLDRLQEEVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLHPPLP 367

Query: 374 LLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGH 433
           L+ P E T  VKL+ Y I  GT V INAWAIGR+   W  +AEEFRPER +N ++D++G 
Sbjct: 368 LMVPHESTHDVKLRDYHIPAGTHVMINAWAIGREAATWGPDAEEFRPERHLNSYVDYRGQ 427

Query: 434 DFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           D ELVPFGAGRR CP + FA+V  +V L
Sbjct: 428 DTELVPFGAGRRICPAISFAVVLDEVVL 447

[Close]

BLAST of Spg007590 vs. ExPASy Swiss-Prot
Match: Q9STK8 (Cytochrome P450 71A25 OS=Arabidopsis thaliana OX=3702 GN=CYP71A25 PE=2 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 8.8e-129
Identity = 236/447 (52.80%), Postives = 323/447 (72.26%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           I L ++I   I  L+K LS    + +PPSPP  LP+ G+L   G H H  +    +RRYG
Sbjct: 6   ILLWSIIFMTILFLKKQLSGKKGK-TPPSPP-GLPLIGNLHQLGRHTHR-SLCDLSRRYG 65

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LLHLG  P ++VSSA++A+EI+KTHD  F+NRP+S  S KLLY ++D+ +APYGEYW
Sbjct: 66  PLMLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYW 125

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPA-SVNLTELFSQLTNNVICRV 193
           RQMKS+ V+HLLSNK VRSFR VREEE+ LM+  I+++ +   N++++   LTN+VICRV
Sbjct: 126 RQMKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLTNDVICRV 185

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKYG   D K L   L E   LLG+FSI +F+PWL W+D I G DA+++++ K+LD 
Sbjct: 186 ALGRKYGGETDFKKLTDRLSE---LLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDD 245

Query: 254 FLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTYT 313
           F ++V++DH + + RD   DL+D LL ++REKS GF +   SIKA+ LD+FV G DT++T
Sbjct: 246 FFEKVVQDHEDGDRRD-GTDLIDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFT 305

Query: 314 LMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVPL 373
           L+EW M+ELLRHP ++ +LQ E+R I   ++ V +DD++ M YLKAVIKE LRLHPP P+
Sbjct: 306 LLEWAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLHPPFPM 365

Query: 374 LSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGHD 433
           ++P E T+ VKL+ Y I  GT+V +NAWAIGR+   W  +AEEF+PER ++  +DF+G +
Sbjct: 366 MAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLDTSVDFRGQN 425

Query: 434 FELVPFGAGRRGCPGVVFAMVNVDVKL 459
           FEL+PFGAGRR CP V FA+V  +V L
Sbjct: 426 FELLPFGAGRRICPAVSFAVVLNEVVL 445

[Close]

BLAST of Spg007590 vs. ExPASy Swiss-Prot
Match: Q9STL2 (Cytochrome P450 71A21 OS=Arabidopsis thaliana OX=3702 GN=CYP71A21 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.7e-127
Identity = 232/447 (51.90%), Postives = 315/447 (70.47%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           I L +LI+F+  L  K         +P SPP +LP+ G+L   G HPH  +  + + RYG
Sbjct: 7   IILQSLIIFITILFFKKQKRGKKSNTPRSPP-RLPLIGNLHQLGHHPHR-SLCSLSHRYG 66

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LLHLG  P +VVSSA++AR+I+KTHD VF++RP+S   +KL Y  +D+  APYGEYW
Sbjct: 67  PLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYW 126

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPA-SVNLTELFSQLTNNVICRV 193
           RQ+KS+ VL LLSNK V SFR VR+EE+ LM+E IQ++ +  VN++EL   LTN+VI R+
Sbjct: 127 RQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQVNVSELLGSLTNDVISRI 186

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKY    D K L   L   M L+G FS+  ++PWLGWID I+GLD ++ +   +LD 
Sbjct: 187 ALGRKYSGETDSKELMKRL---MMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDE 246

Query: 254 FLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTYT 313
           FL++V++DHV+ +   +  D VD+LL IQREKS+GF ++   IKA++LD+ VAG D++Y 
Sbjct: 247 FLEKVVQDHVDGDG--QRTDFVDVLLRIQREKSIGFEIDRLCIKAIVLDVLVAGTDSSYA 306

Query: 314 LMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVPL 373
           LM+W M+ELLRHP+ ++ LQ E+R I      V ++D++ M YLKAVIKET RLHPP+PL
Sbjct: 307 LMDWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLHPPLPL 366

Query: 374 LSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGHD 433
           L+P E  Q V L  Y I  GT+V INAWAIGR+   W  +AE+FRPER ++  +DF+GH+
Sbjct: 367 LAPHESIQDVILGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLDSSVDFRGHN 426

Query: 434 FELVPFGAGRRGCPGVVFAMVNVDVKL 459
           FELVPFGAGRR CP + FA+V ++V L
Sbjct: 427 FELVPFGAGRRICPAISFAVVLIEVAL 446

[Close]

BLAST of Spg007590 vs. ExPASy Swiss-Prot
Match: Q9STK7 (Cytochrome P450 71A26 OS=Arabidopsis thaliana OX=3702 GN=CYP71A26 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.8e-127
Identity = 235/455 (51.65%), Postives = 323/455 (70.99%), Query Frame = 0


Query: 6   LLHLFFHPISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTF 65
           ++ +FF   S+  ++  +IF  QK     ++  SPP     LP+ G+L   G HPH  + 
Sbjct: 1   MMIMFFLLCSIIFVVTIIIFRKQKRGKKRNTLPSPPG----LPLIGNLHQLGRHPHR-SL 60

Query: 66  QAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDIT 125
            + + RYGPL LLH G  P +VVSSAELAR+++KTHD VF++RP+S   +KLLY   D+ 
Sbjct: 61  CSLSHRYGPLMLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVA 120

Query: 126 AAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRN-PASVNLTELFSQL 185
           +APYGEYWRQMKS+ VLHL SNK VRSFR VREEE+ LM+E I+++    VNL+++   L
Sbjct: 121 SAPYGEYWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSL 180

Query: 186 TNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIE 245
           TN+VIC+VALGRKYG   D K L   L    KLLG+FS+ +++PWL WID I GLD ++E
Sbjct: 181 TNDVICKVALGRKYGGETDFKELMERL---NKLLGTFSVGSYVPWLAWIDWIRGLDCQLE 240

Query: 246 RVAKELDAFLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFV 305
           + A ++D F +RV++DHV+  NRD   D VD+LL IQR+K++GF +N  SIKA+++++FV
Sbjct: 241 KTANDVDKFFERVVQDHVD-GNRD-MTDFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFV 300

Query: 306 AGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETL 365
            G DT+ TLMEW M+ELLRHP  +K+LQ E+R I  D++ V +++++ M YLKAVIKE L
Sbjct: 301 GGTDTSSTLMEWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEAL 360

Query: 366 RLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNR 425
           RLHPP+PL+ P E TQ V+L  + I  GT+V INAWAIGR+   W  + EEFRPER ++ 
Sbjct: 361 RLHPPLPLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDS 420

Query: 426 WMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
            +D++G  FEL+PFG+GRR CP + FA+V  +V L
Sbjct: 421 SVDYRGQAFELIPFGSGRRICPAISFAVVLNEVVL 445

[Close]

BLAST of Spg007590 vs. ExPASy TrEMBL
Match: A0A6J1E1I6 (cytochrome P450 71A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.5e-195
Identity = 343/465 (73.76%), Postives = 397/465 (85.38%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR+IMKTHDLVF+NRPKS  +DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDIMKTHDLVFANRPKSSMADKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED  NL S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDLINLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DA+ +RVAKELD FL RVI+DHV+ ENRDE    HKDLVD+LL IQR  ++GFPL MDSI
Sbjct: 241 DARAKRVAKELDVFLGRVIQDHVDSENRDENSGKHKDLVDVLLWIQRNNTIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG +TT T+MEWTMSELL+HP+AMKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFAAGTETTSTVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KLKGYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKGHDF+L+PFGAGRRGCPG+ FA V  ++ L
Sbjct: 421 EFRPERFMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIAL 464

[Close]

BLAST of Spg007590 vs. ExPASy TrEMBL
Match: A0A6J1E1E9 (cytochrome P450 71A3-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.6e-194
Identity = 344/463 (74.30%), Postives = 395/463 (85.31%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR+IMKTHDLVF+NRPKS  +DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDIMKTHDLVFANRPKSSMADKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED  NL S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDLINLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DA+ +RVAKELD FL RVI+DHV+ ENRDE    HKDLVD+LL IQR  ++GFPL MDSI
Sbjct: 241 DARAKRVAKELDVFLGRVIQDHVDSENRDENSGKHKDLVDVLLWIQRNNTIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHY 360
           KAL+LDMF AG DT  T++EW MSELL+HP+AMKKL+SEIREI+GD  YV++DDL+KMHY
Sbjct: 301 KALILDMFSAGTDTISTVLEWAMSELLKHPEAMKKLKSEIREISGDH-YVNEDDLDKMHY 360

Query: 361 LKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAEEF 420
           LKAVIKETLRLHPP+PLL PRE TQY+KL+GYDIKP TRV INAWAIGRDP++WEEAEEF
Sbjct: 361 LKAVIKETLRLHPPIPLLVPRESTQYIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEF 420

Query: 421 RPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           RPERFMN  +DFKG DFEL+PFGAGRRGCPGV FA V  ++ L
Sbjct: 421 RPERFMNSSIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGL 461

[Close]

BLAST of Spg007590 vs. ExPASy TrEMBL
Match: A0A6J1E4T4 (cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.8e-194
Identity = 342/465 (73.55%), Postives = 395/465 (84.95%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+ELAR++MKTHDLVF+NRPKS K DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSELARDVMKTHDLVFANRPKSTKHDKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            SK + AAPYGEYWRQMKS+ VLHLLSN+RV+SFR VREEEVKLMIE IQ+NP SVNLTE
Sbjct: 121 NSKVVAAAPYGEYWRQMKSIDVLHLLSNRRVQSFRSVREEEVKLMIEKIQQNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTN+V+CRVALGRK+  GEDG  L S+LLE ++LLGSF IR+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNDVVCRVALGRKFDAGEDGMKLRSLLLELLELLGSFGIRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DAK +RVAKELD FLDRVI+D V+ ENRDE    HKDLVD+LL +QR  S+GFPL MDSI
Sbjct: 241 DAKGKRVAKELDVFLDRVIQDRVHSENRDENSGKHKDLVDVLLWVQRNNSIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG +TT T+MEWTMSELL+HP+AMKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFAAGTETTSTVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KLKGYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKGHDF+L+PFGAGRRGCPG+ FA V  ++ L
Sbjct: 421 EFRPERFMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIAL 464

[Close]

BLAST of Spg007590 vs. ExPASy TrEMBL
Match: A0A6J1E6T0 (cytochrome P450 71A3-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 6.2e-194
Identity = 341/465 (73.33%), Postives = 395/465 (84.95%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           MDCDLL+ L  HP S+   IV  I+LL    SSS  E +SPPSPP+KLPIFGHLL  GSH
Sbjct: 1   MDCDLLVALIGHPASVAGFIVLFIWLLFCQKSSSQEEQSSPPSPPRKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR+IMKTHDLVF+NRPKS  +DKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDIMKTHDLVFANRPKSSMADKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED  NL S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDLINLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DA+ +RVAKELD FL RVI+DHV+ ENRDE    HKDLVD+LL IQR  ++GFPL MDSI
Sbjct: 241 DARAKRVAKELDVFLGRVIQDHVDSENRDENSGKHKDLVDVLLWIQRNNTIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRA--YVDDDDLEKM 360
           KAL+LDMF AG DT  T++EW MSELL+HP+AMKKL+SEIREI+GD    YV++DDL+KM
Sbjct: 301 KALILDMFSAGTDTISTVLEWAMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKM 360

Query: 361 HYLKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAE 420
           HYLKAV+KETLRLHPP+PLL PRE TQ +KLKGYDIKP TRV INAWAIGRDP++W+EAE
Sbjct: 361 HYLKAVLKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAE 420

Query: 421 EFRPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           EFRPERFMN  +DFKGHDF+L+PFGAGRRGCPG+ FA V  ++ L
Sbjct: 421 EFRPERFMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIAL 464

[Close]

BLAST of Spg007590 vs. ExPASy TrEMBL
Match: A0A6J1JG96 (cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.2e-192
Identity = 341/463 (73.65%), Postives = 394/463 (85.10%), Query Frame = 0


Query: 1   MDCDLLLHLFFHPISLTALIVFLIFLLQKWLSSSSSE-ASPPSPPKKLPIFGHLLSFGSH 60
           M+ DLL+ L  HP+S+   IV  I+LL +  SSS  E +SPPSPP KLPIFGHLL  GSH
Sbjct: 1   MEGDLLVALIGHPVSVAGFIVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSH 60

Query: 61  PHHLTFQAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLY 120
           P H+T Q+YARR+GPLFLLHLGSKPTIVVSS+E+AR++MKTHDLVF+NRPKS  SDKLLY
Sbjct: 61  P-HITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLY 120

Query: 121 GSKDITAAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPASVNLTE 180
            + DI+AAPYGEYWRQM+S+ VLHLLSNKRV+SFR VREEEVKLMIE IQRNP SVNLTE
Sbjct: 121 NASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTE 180

Query: 181 LFSQLTNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGL 240
            FS LTNNV+CRVALG KYGVGED   L S+LLE ++LLGSF +R+FIPWLGWID I+GL
Sbjct: 181 TFSGLTNNVVCRVALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGL 240

Query: 241 DAKIERVAKELDAFLDRVIEDHVNPENRDE----HKDLVDILLCIQREKSMGFPLNMDSI 300
           DAK +RVAKELD FLDRVI+DHV+ ENRDE    H DLVD+LL IQR  S+GFPL MDSI
Sbjct: 241 DAKAKRVAKELDVFLDRVIQDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSI 300

Query: 301 KALLLDMFVAGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHY 360
           KAL+LDMF AG DTT T++EW MSEL +HP+AMKKL+SEIREI+GD+ YV++DDL+KMHY
Sbjct: 301 KALILDMFSAGTDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQ-YVNEDDLDKMHY 360

Query: 361 LKAVIKETLRLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLWEEAEEF 420
           LKAV+KETLRLHPP+PLL PRE TQ +KL+GYDIKP TRV INAWAIGRDP++WEEAEEF
Sbjct: 361 LKAVMKETLRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEF 420

Query: 421 RPERFMNRWMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           RPERFMN  +DFKG DFEL+PFGAGRRGCPGV FA V  ++ L
Sbjct: 421 RPERFMNSSIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGL 461

[Close]

BLAST of Spg007590 vs. TAIR 10
Match: AT3G48290.1 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 463.4 bits (1191), Expect = 2.2e-130
Identity = 239/448 (53.35%), Postives = 315/448 (70.31%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           I L   I+ +  L  K  S      +PPSPP +LP+  +L   G HPH  +  + + RYG
Sbjct: 8   ILLLCSIILITILFFKKQSRGKKSNAPPSPP-RLPLIRNLHQLGRHPHR-SLCSLSHRYG 67

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LLH GS P +VVSSA+ A++++KTHD VF++RP+S   DK+ Y  +D+  APYGEYW
Sbjct: 68  PLMLLHFGSVPVLVVSSADAAKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYW 127

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQ-RNPASVNLTELFSQLTNNVICRV 193
           RQMKS+ VLHL SNK VRSFR VR+EE+ LMIE I+  +   +NL+E+   LTNNVICRV
Sbjct: 128 RQMKSVCVLHLFSNKMVRSFRDVRQEEISLMIEKIRISSSLRINLSEILVNLTNNVICRV 187

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKYG   D K+L   L    +LLG FS+ +++ WL WID I GLD ++ +++ +LD 
Sbjct: 188 ALGRKYGGKTDFKDLMKRL---TRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDE 247

Query: 254 FLDRVIEDHVNPENRDEHK-DLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTY 313
           FL+RV++DHV   + D HK D VD LL I+REKS+GF ++  SIKA++LD+FV   DTTY
Sbjct: 248 FLERVVQDHV---DGDGHKNDFVDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTY 307

Query: 314 TLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVP 373
           TL+EW M+ELL H + + +LQ E+R +  D++ V +DDL+ M YLKAVIKETLRLHPP+P
Sbjct: 308 TLLEWAMTELLCHHECLDRLQEEVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLHPPLP 367

Query: 374 LLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGH 433
           L+ P E T  VKL+ Y I  GT V INAWAIGR+   W  +AEEFRPER +N ++D++G 
Sbjct: 368 LMVPHESTHDVKLRDYHIPAGTHVMINAWAIGREAATWGPDAEEFRPERHLNSYVDYRGQ 427

Query: 434 DFELVPFGAGRRGCPGVVFAMVNVDVKL 459
           D ELVPFGAGRR CP + FA+V  +V L
Sbjct: 428 DTELVPFGAGRRICPAISFAVVLDEVVL 447

[Close]

BLAST of Spg007590 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 461.8 bits (1187), Expect = 6.3e-130
Identity = 236/447 (52.80%), Postives = 323/447 (72.26%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           I L ++I   I  L+K LS    + +PPSPP  LP+ G+L   G H H  +    +RRYG
Sbjct: 6   ILLWSIIFMTILFLKKQLSGKKGK-TPPSPP-GLPLIGNLHQLGRHTHR-SLCDLSRRYG 65

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LLHLG  P ++VSSA++A+EI+KTHD  F+NRP+S  S KLLY ++D+ +APYGEYW
Sbjct: 66  PLMLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYW 125

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPA-SVNLTELFSQLTNNVICRV 193
           RQMKS+ V+HLLSNK VRSFR VREEE+ LM+  I+++ +   N++++   LTN+VICRV
Sbjct: 126 RQMKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLTNDVICRV 185

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKYG   D K L   L E   LLG+FSI +F+PWL W+D I G DA+++++ K+LD 
Sbjct: 186 ALGRKYGGETDFKKLTDRLSE---LLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDD 245

Query: 254 FLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTYT 313
           F ++V++DH + + RD   DL+D LL ++REKS GF +   SIKA+ LD+FV G DT++T
Sbjct: 246 FFEKVVQDHEDGDRRD-GTDLIDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFT 305

Query: 314 LMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVPL 373
           L+EW M+ELLRHP ++ +LQ E+R I   ++ V +DD++ M YLKAVIKE LRLHPP P+
Sbjct: 306 LLEWAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLHPPFPM 365

Query: 374 LSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGHD 433
           ++P E T+ VKL+ Y I  GT+V +NAWAIGR+   W  +AEEF+PER ++  +DF+G +
Sbjct: 366 MAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLDTSVDFRGQN 425

Query: 434 FELVPFGAGRRGCPGVVFAMVNVDVKL 459
           FEL+PFGAGRR CP V FA+V  +V L
Sbjct: 426 FELLPFGAGRRICPAVSFAVVLNEVVL 445

[Close]

BLAST of Spg007590 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-128
Identity = 232/447 (51.90%), Postives = 315/447 (70.47%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           I L +LI+F+  L  K         +P SPP +LP+ G+L   G HPH  +  + + RYG
Sbjct: 7   IILQSLIIFITILFFKKQKRGKKSNTPRSPP-RLPLIGNLHQLGHHPHR-SLCSLSHRYG 66

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LLHLG  P +VVSSA++AR+I+KTHD VF++RP+S   +KL Y  +D+  APYGEYW
Sbjct: 67  PLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYW 126

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPA-SVNLTELFSQLTNNVICRV 193
           RQ+KS+ VL LLSNK V SFR VR+EE+ LM+E IQ++ +  VN++EL   LTN+VI R+
Sbjct: 127 RQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQVNVSELLGSLTNDVISRI 186

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKY    D K L   L   M L+G FS+  ++PWLGWID I+GLD ++ +   +LD 
Sbjct: 187 ALGRKYSGETDSKELMKRL---MMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDE 246

Query: 254 FLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTYT 313
           FL++V++DHV+ +   +  D VD+LL IQREKS+GF ++   IKA++LD+ VAG D++Y 
Sbjct: 247 FLEKVVQDHVDGDG--QRTDFVDVLLRIQREKSIGFEIDRLCIKAIVLDVLVAGTDSSYA 306

Query: 314 LMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVPL 373
           LM+W M+ELLRHP+ ++ LQ E+R I      V ++D++ M YLKAVIKET RLHPP+PL
Sbjct: 307 LMDWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLHPPLPL 366

Query: 374 LSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGHD 433
           L+P E  Q V L  Y I  GT+V INAWAIGR+   W  +AE+FRPER ++  +DF+GH+
Sbjct: 367 LAPHESIQDVILGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLDSSVDFRGHN 426

Query: 434 FELVPFGAGRRGCPGVVFAMVNVDVKL 459
           FELVPFGAGRR CP + FA+V ++V L
Sbjct: 427 FELVPFGAGRRICPAISFAVVLIEVAL 446

[Close]

BLAST of Spg007590 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 456.8 bits (1174), Expect = 2.0e-128
Identity = 235/455 (51.65%), Postives = 323/455 (70.99%), Query Frame = 0


Query: 6   LLHLFFHPISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTF 65
           ++ +FF   S+  ++  +IF  QK     ++  SPP     LP+ G+L   G HPH  + 
Sbjct: 1   MMIMFFLLCSIIFVVTIIIFRKQKRGKKRNTLPSPPG----LPLIGNLHQLGRHPHR-SL 60

Query: 66  QAYARRYGPLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDIT 125
            + + RYGPL LLH G  P +VVSSAELAR+++KTHD VF++RP+S   +KLLY   D+ 
Sbjct: 61  CSLSHRYGPLMLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVA 120

Query: 126 AAPYGEYWRQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRN-PASVNLTELFSQL 185
           +APYGEYWRQMKS+ VLHL SNK VRSFR VREEE+ LM+E I+++    VNL+++   L
Sbjct: 121 SAPYGEYWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSL 180

Query: 186 TNNVICRVALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIE 245
           TN+VIC+VALGRKYG   D K L   L    KLLG+FS+ +++PWL WID I GLD ++E
Sbjct: 181 TNDVICKVALGRKYGGETDFKELMERL---NKLLGTFSVGSYVPWLAWIDWIRGLDCQLE 240

Query: 246 RVAKELDAFLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFV 305
           + A ++D F +RV++DHV+  NRD   D VD+LL IQR+K++GF +N  SIKA+++++FV
Sbjct: 241 KTANDVDKFFERVVQDHVD-GNRD-MTDFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFV 300

Query: 306 AGKDTTYTLMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETL 365
            G DT+ TLMEW M+ELLRHP  +K+LQ E+R I  D++ V +++++ M YLKAVIKE L
Sbjct: 301 GGTDTSSTLMEWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEAL 360

Query: 366 RLHPPVPLLSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNR 425
           RLHPP+PL+ P E TQ V+L  + I  GT+V INAWAIGR+   W  + EEFRPER ++ 
Sbjct: 361 RLHPPLPLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDS 420

Query: 426 WMDFKGHDFELVPFGAGRRGCPGVVFAMVNVDVKL 459
            +D++G  FEL+PFG+GRR CP + FA+V  +V L
Sbjct: 421 SVDYRGQAFELIPFGSGRRICPAISFAVVLNEVVL 445

[Close]

BLAST of Spg007590 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 456.4 bits (1173), Expect = 2.6e-128
Identity = 231/447 (51.68%), Postives = 316/447 (70.69%), Query Frame = 0


Query: 14  ISLTALIVFLIFLLQKWLSSSSSEASPPSPPKKLPIFGHLLSFGSHPHHLTFQAYARRYG 73
           +SL   I  L F+ QK    S++ ASPP    +LP+ G+L   G HPH  +  + + RYG
Sbjct: 10  LSLIIFITILFFIKQKKGKKSNTPASPP----RLPLIGNLHQLGRHPHR-SLCSLSNRYG 69

Query: 74  PLFLLHLGSKPTIVVSSAELAREIMKTHDLVFSNRPKSIKSDKLLYGSKDITAAPYGEYW 133
           PL LL  G  P +VVSSA++AR+I+KT+D VF++RP+S   +K+ Y ++D+  APYGEYW
Sbjct: 70  PLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYW 129

Query: 134 RQMKSLAVLHLLSNKRVRSFRFVREEEVKLMIENIQRNPA-SVNLTELFSQLTNNVICRV 193
           RQMKS+ VLHLL+NK VRSFR VR+EE+ LM+E IQ++ +  VNL+EL   LTN+VI RV
Sbjct: 130 RQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQVNLSELLGSLTNDVISRV 189

Query: 194 ALGRKYGVGEDGKNLHSVLLEGMKLLGSFSIRNFIPWLGWIDAITGLDAKIERVAKELDA 253
           ALGRKY    D K L   L    KLLG F +  ++PWL WID I+GLD ++++   +LD 
Sbjct: 190 ALGRKYSDETDFKELMKRL---TKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDE 249

Query: 254 FLDRVIEDHVNPENRDEHKDLVDILLCIQREKSMGFPLNMDSIKALLLDMFVAGKDTTYT 313
           FL++V++DH   +   +  D VD+LL IQREKS+GF ++  SIKA++LD+ V G DT+Y 
Sbjct: 250 FLEKVVQDH--EDGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYA 309

Query: 314 LMEWTMSELLRHPDAMKKLQSEIREITGDRAYVDDDDLEKMHYLKAVIKETLRLHPPVPL 373
           LMEW M+ELL  P+ + +LQ E+R I    + V +DD++ M+YLKAVIKET+RLHPP+PL
Sbjct: 310 LMEWAMTELLHRPECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLHPPLPL 369

Query: 374 LSPREPTQYVKLKGYDIKPGTRVTINAWAIGRDPKLW-EEAEEFRPERFMNRWMDFKGHD 433
           + P E TQ V+L  Y I  GT+V INAWAIGR+   W  +AE+FRPER +N  +DF+GH+
Sbjct: 370 MVPHESTQDVRLGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLNSSVDFRGHN 429

Query: 434 FELVPFGAGRRGCPGVVFAMVNVDVKL 459
           FEL+PFGAGRR CP + FA++ ++V L
Sbjct: 430 FELIPFGAGRRICPAISFAVILIEVTL 446

The following BLAST results are available for this feature:

BLAST of Spg007590 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022921784.1	5.2e-195	73.76	cytochrome P450 71A3-like isoform X2 [Cucurbita moschata]	[more]
XP_022921787.1	3.4e-194	74.30	cytochrome P450 71A3-like isoform X4 [Cucurbita moschata]	[more]
XP_022921783.1	5.8e-194	73.55	cytochrome P450 71A22-like isoform X1 [Cucurbita moschata]	[more]
XP_022921785.1	1.3e-193	73.33	cytochrome P450 71A3-like isoform X3 [Cucurbita moschata]	[more]
XP_023515571.1	4.9e-193	72.47	cytochrome P450 71A24-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg007590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q6L2	4.4e-144	55.75	Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1	[more]
Q9STK9	3.0e-129	53.35	Cytochrome P450 71A24 OS=Arabidopsis thaliana OX=3702 GN=CYP71A24 PE=2 SV=3	[more]
Q9STK8	8.8e-129	52.80	Cytochrome P450 71A25 OS=Arabidopsis thaliana OX=3702 GN=CYP71A25 PE=2 SV=1	[more]
Q9STL2	1.7e-127	51.90	Cytochrome P450 71A21 OS=Arabidopsis thaliana OX=3702 GN=CYP71A21 PE=2 SV=1	[more]
Q9STK7	2.8e-127	51.65	Cytochrome P450 71A26 OS=Arabidopsis thaliana OX=3702 GN=CYP71A26 PE=3 SV=1	[more]

BLAST of Spg007590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E1I6	2.5e-195	73.76	cytochrome P450 71A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114299...	[more]
A0A6J1E1E9	1.6e-194	74.30	cytochrome P450 71A3-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC1114299...	[more]
A0A6J1E4T4	2.8e-194	73.55	cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429...	[more]
A0A6J1E6T0	6.2e-194	73.33	cytochrome P450 71A3-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114299...	[more]
A0A6J1JG96	1.2e-192	73.65	cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1	[more]

BLAST of Spg007590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48290.1	2.2e-130	53.35	cytochrome P450, family 71, subfamily A, polypeptide 24	[more]
AT3G48280.1	6.3e-130	52.80	cytochrome P450, family 71, subfamily A, polypeptide 25	[more]
AT3G48320.1	1.2e-128	51.90	cytochrome P450, family 71, subfamily A, polypeptide 21	[more]
AT3G48270.1	2.0e-128	51.65	cytochrome P450, family 71, subfamily A, polypeptide 26	[more]
AT3G48310.1	2.6e-128	51.68	cytochrome P450, family 71, subfamily A, polypeptide 22	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 294..311 score: 35.08 coord: 314..340 score: 31.54 coord: 69..88 score: 38.72 coord: 184..202 score: 25.03 coord: 434..444 score: 55.33 coord: 357..375 score: 42.78 coord: 398..422 score: 37.24 coord: 93..114 score: 23.25
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 305..322 score: 35.45 coord: 435..444 score: 58.17 coord: 444..455 score: 31.04 coord: 358..369 score: 52.33
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 41..456 e-value: 2.0E-97 score: 326.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 27..458 e-value: 1.9E-122 score: 411.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 40..454
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 22..454 e-value: 1.9E-18 score: 64.5
None	No IPR available	PIRSR	PIRSR000209-1	PIRSR000209-1	coord: 42..453 e-value: 2.0E-39 score: 132.8
None	No IPR available	PANTHER	PTHR47956:SF4	CYTOCHROME P450 71A21-RELATED	coord: 17..456
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 17..456
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 437..446

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg007590.1	Spg007590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen