Spg007517 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg007517 vs. NCBI nr
Match: XP_023516208.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914.1 bits (2361), Expect = 5.2e-262
Identity = 438/482 (90.87%), Postives = 461/482 (95.64%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASVPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEAV +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Spg007517 vs. NCBI nr
Match: XP_022921757.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 907.9 bits (2345), Expect = 3.7e-260
Identity = 436/482 (90.46%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEAV +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Spg007517 vs. NCBI nr
Match: XP_023516209.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.5 bits (2344), Expect = 4.8e-260
Identity = 437/482 (90.66%), Postives = 460/482 (95.44%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASVPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEA  +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Spg007517 vs. NCBI nr
Match: KAG6589732.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905.2 bits (2338), Expect = 2.4e-259
Identity = 436/482 (90.46%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ PP+N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEA  +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Spg007517 vs. NCBI nr
Match: XP_022921758.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 901.4 bits (2328), Expect = 3.5e-258
Identity = 435/482 (90.25%), Postives = 458/482 (95.02%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEA  +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Spg007517 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 4.6e-189
Identity = 314/466 (67.38%), Postives = 381/466 (81.76%), Query Frame = 0

Query: 22  SLKGCG---LSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKDPAAGASATAPPENVAPP- 81
           +++GCG   L+ + IDK +L+ +L MP+YLR+A+RD I+ KD    +     P   A   
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 82  PPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADR 141
            P AP+VVFINP+SGGRHG  LK+RLQQL++EEQVFDL +VKPHEFV+YGL CL+ LA +
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 142 GDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 201
           GD CA++ R+KIR+MVAGGDGTVGWVLG L EL+K  +  + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 202 WGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKVEPPHSMKLT-EESDRNE 261
           WGGSFPFAW+SA+KR+LHRAT G +  LDSW + +SMP+GE V+PP+S+K T EE+  ++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 262 AVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYS 321
           A   LD  GD+P K   YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS
Sbjct: 270 A---LDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYS 329

Query: 322 SYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYAS 381
           SYSCTQGWF T C  +P LRGL+NI++IH+KK NCSEWE+I +P SVRSIV LNL+NY S
Sbjct: 330 SYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGS 389

Query: 382 GRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRI 441
           GR+PWG  +P+YLEKRGF+EAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR 
Sbjct: 390 GRHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRF 449

Query: 442 EIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMINGD 483
           E+RGG  K A+LQMDGEPWKQP+  D+STFVEIK+VP QSLMING+
Sbjct: 450 ELRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Spg007517 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 2.8e-186
Identity = 311/476 (65.34%), Postives = 378/476 (79.41%), Query Frame = 0

Query: 13  IAARSSLFDS--LKGCGLSG---IRIDKHQLKTKLMMPQYLRLALRDSIRFKDPAA-GAS 72
           +A+R S  DS  ++GCGL+    + +DK +L+ +LMMP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSAIPDH 75

Query: 73  ATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQY 132
              P   VA   P AP+VVFINP SGGRHG  LK+RLQQL+SEEQVFDL +VKPHEFV+Y
Sbjct: 76  LLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRY 135

Query: 133 GLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPL 192
           GL CLE +A  GD CAK+ R ++R+MVAGGDGTVGWVLG L EL K  +  + PVG+IPL
Sbjct: 136 GLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPL 195

Query: 193 GTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKVEPPHSM 252
           GTGNDLSRSFGWGGSFPFAW+SAVKR+LHRA+ G V  LDSW + +SMP+GE V+PP+S+
Sbjct: 196 GTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSL 255

Query: 253 KLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQ 312
           K  EE   NE    LD   D P     YEGVFYNY SIGMDAQVAYGFHHLRN +PYLAQ
Sbjct: 256 KPAEE---NELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQ 315

Query: 313 GPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSI 372
           GP++NK+IYSS+ C+QGWF T C  DP LRGL+NI++IH+KK+NCS+WE+I +P +VRSI
Sbjct: 316 GPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSI 375

Query: 373 VALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAK 432
           VALNLH+Y SG +PWG  KP+YLEKRGF+EAH DDGL+EIFG KQGWH SFVM ++ISAK
Sbjct: 376 VALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAK 435

Query: 433 HIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMINGD 483
           HIAQAAA+R E+RGG  + A+LQMDGEPWKQP+S ++STFVEIK+VP QSLMIN +
Sbjct: 436 HIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of Spg007517 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 3.4e-184
Identity = 299/458 (65.28%), Postives = 376/458 (82.10%), Query Frame = 0

Query: 28  LSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKDPAAGASAT-APPENVAPPPPEAPLVVF 87
           L+ IR+ K +L+ ++M+PQYLR+A+RD I  KD +  AS++ APP       PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 88  INPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTR 147
           +NP+SGGR G  +K+RLQ LISEEQV+DL +VKP+EF++YGL CLE  A RGD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 148 QKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 207
           +K+R++VAGGDGTVGWVLG L EL  Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 208 KSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKVEPPHSMKLTEES--DRNEAVHELDDR 267
           KSA+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+S+K T+E   D+N     L+  
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQN-----LEIE 274

Query: 268 GDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGW 327
           G++P   + YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYS Y C+QGW
Sbjct: 275 GEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGW 334

Query: 328 FFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKP 387
           F T C  DP LRGLKNI+ +H+KK++ SEWE++P+P SVR++VALNLH+Y SGRNPWG  
Sbjct: 335 FLTHCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNL 394

Query: 388 KPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRK 447
           K +YLEKRGF+EA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K
Sbjct: 395 KQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWK 454

Query: 448 KAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMINGD 483
            A++QMDGEPWKQP+++D+STFV+IKRVP QSL++ GD
Sbjct: 455 DAFMQMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of Spg007517 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.9e-73
Identity = 156/422 (36.97%), Postives = 229/422 (54.27%), Query Frame = 0

Query: 79  PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRC-LEILADR 138
           P  P+VVFIN RSGG+ G  L +  ++L+++ QVFDL +  P E V + L C  E L   
Sbjct: 42  PCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDLSEEAP-EKVLHRLYCNFEKLKSN 101

Query: 139 GDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 198
           GD  A Q +  +R++VAGGDGT  W+LG +++L K    P  P+  +PLGTGN+L  SFG
Sbjct: 102 GDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-KLSHPP--PIATVPLGTGNNLPFSFG 161

Query: 199 WGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEK--------VEPPHSMKLT 258
           WG   P   + AVK  L +    +  ++DSWH+ + M A ++        +E PHS+   
Sbjct: 162 WGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIMRMRAPQEGPCEPIAPLELPHSL--- 221

Query: 259 EESDRNEAVHELDDRGDLPTK-LDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 318
                  A H +     L  +    Y G F+NYFS+GMDAQV+Y FH  R + P   +  
Sbjct: 222 ------HAFHRVSGSDSLNMEGYHTYRGGFWNYFSMGMDAQVSYEFHSERKRNPEKFKNQ 281

Query: 319 VTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVA 378
           +TN+  Y+     QGWF  S +  P+ R +  + ++ + K    +WE++ IP S+RSIV 
Sbjct: 282 LTNQSTYAKLGLKQGWFAASLT-HPSSRNIAQLAKVRIMKRPGGQWEELKIPRSIRSIVC 341

Query: 379 LNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 438
           LNL +++ G NPWG P    ++ R       DDGL+E+ G +  WH   ++        +
Sbjct: 342 LNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLLAPNGHGTRL 401

Query: 439 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFST-FVEIKRV---------PCQSLM 481
           AQA  IR E   G  +  ++++DGEPWKQP+ KD  T  VEI  +         PC+S  
Sbjct: 402 AQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDDDTVVVEISHLRQVTMLASDPCKSKS 449

BLAST of Spg007517 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.3e-71
Identity = 158/438 (36.07%), Postives = 236/438 (53.88%), Query Frame = 0

Query: 41  KLMMPQYLRLALRDSIRFKDPAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLK 100
           K  +P Y+ L+  D     +P A  S T  PEN        P++VFIN +SGG+ G +L 
Sbjct: 20  KFFIPSYV-LSPED-----EPEAQISCTTAPEN--------PILVFINSKSGGQLGAELI 79

Query: 101 QRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTV 160
              + L++++QVFDL    P + +Q     LE L D  D+ A + R K++++VAGGDGT 
Sbjct: 80  LTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTA 139

Query: 161 GWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTG 220
           GW+LG +++L   +     P+  +PLGTGN+L  +FGWG   P   +S+V+  L +    
Sbjct: 140 GWLLGVVSDLNLSNPP---PIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINA 199

Query: 221 QVRSLDSWHVSLSMPAGEK------VEPPHSMKLTEESDRN--EAVHELDDRGDLPTKLD 280
           +   +D+W + + M   ++      ++ PHS+     SD+   E  H             
Sbjct: 200 KEMKIDNWKILMRMKHPKEGSCDITLKLPHSLPRIFPSDQENMEGYH------------- 259

Query: 281 CYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKD 340
            Y G F+NYFS+GMDAQV+Y FH  R   P   +  + N+  Y   SCTQGWFF S    
Sbjct: 260 TYRGGFWNYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-H 319

Query: 341 PNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKR 400
           P+ + +  + +I +   N  +W  + IP S+RSIV LNL +++ G NPWG P P+    R
Sbjct: 320 PSSQNIAKLAKIQICDRN-GQWNDLHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDR 379

Query: 401 GFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDG 460
                  DDGL+EI G +  WH   ++        +AQA  +R+E + G  K AY+++DG
Sbjct: 380 SLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDG 423

Query: 461 EPWKQPI-SKDFSTFVEI 470
           EPWKQP+ S D +  VEI
Sbjct: 440 EPWKQPLPSNDETVMVEI 423

BLAST of Spg007517 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.8e-260
Identity = 436/482 (90.46%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEAV +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Spg007517 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 1.7e-258
Identity = 435/482 (90.25%), Postives = 458/482 (95.02%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEA  +LD++GDLPTKL  YEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Spg007517 vs. ExPASy TrEMBL
Match: A0A6J1JJR6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.9e-257
Identity = 431/482 (89.42%), Postives = 457/482 (94.81%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLA+RDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASLPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL  QDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGE+V
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEAV +LD++GDLPTKL  Y GVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RP LA+GP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPKLARGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Spg007517 vs. ExPASy TrEMBL
Match: A0A0A0LZ78 (Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 430/485 (88.66%), Postives = 453/485 (93.40%), Query Frame = 0

Query: 1   MDHSR---GGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIR 60
           MDHS    G  D+V++AARSS  DSLKGCGLSGIRIDKH+L TK++MPQYLR ALRDSIR
Sbjct: 105 MDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 164

Query: 61  FKDPAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLD 120
            KDP AGASAT PP+N+  P PE PLVVFINPRSGGRHGQKLKQRLQ+LISEEQVFDLLD
Sbjct: 165 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 224

Query: 121 VKPHEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKP 180
           VKPHEFVQYGLRCLEILAD GD CAKQTRQKIR+MVAGGDGTVGWVLGSLAELYKQDR P
Sbjct: 225 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 284

Query: 181 VLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAG 240
           VLPVGIIPLGTGNDL+RSFGWGGSFPFAWKSAVKRSL RATTGQV SLDSWHVSLSMPAG
Sbjct: 285 VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG 344

Query: 241 EKVEPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHL 300
           EKVEPPHS+KLTEESD NEAVH+LDD  D PTKL C+EGVFYNYFSIGMDAQVAYGFHHL
Sbjct: 345 EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL 404

Query: 301 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQI 360
           RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCS +PNL GLKNI+RIH+KKINC+EWE++
Sbjct: 405 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV 464

Query: 361 PIPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSF 420
           P+PSSVRSIVALNLHNYASGRNPWGK KPEYLEKRGF+EAHADDGLLEIFGLKQGWHTSF
Sbjct: 465 PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 524

Query: 421 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSL 480
           VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISK+ STFVEIKRVP QSL
Sbjct: 525 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL 584

Query: 481 MINGD 483
           MINGD
Sbjct: 585 MINGD 589

BLAST of Spg007517 vs. ExPASy TrEMBL
Match: A0A6J1JHE1 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 430/482 (89.21%), Postives = 456/482 (94.61%), Query Frame = 0

Query: 1   MDHSRGGADDVSIAARSSLFDSLKGCGLSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D VSIAARSSLFDSLKGCGLSGI IDK +L TK+MMPQYLRLA+RDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKD 60

Query: 61  PAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+ PP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASLPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL  QDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV LS+PAGE+V
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERV 240

Query: 241 EPPHSMKLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESD NEA  +LD++GDLPTKL  Y GVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RP LA+GP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPKLARGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGF+EAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KDFST+VEIKRVP QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Spg007517 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 662.1 bits (1707), Expect = 3.3e-190
Identity = 314/466 (67.38%), Postives = 381/466 (81.76%), Query Frame = 0

Query: 22  SLKGCG---LSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKDPAAGASATAPPENVAPP- 81
           +++GCG   L+ + IDK +L+ +L MP+YLR+A+RD I+ KD    +     P   A   
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 82  PPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADR 141
            P AP+VVFINP+SGGRHG  LK+RLQQL++EEQVFDL +VKPHEFV+YGL CL+ LA +
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 142 GDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 201
           GD CA++ R+KIR+MVAGGDGTVGWVLG L EL+K  +  + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 202 WGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKVEPPHSMKLT-EESDRNE 261
           WGGSFPFAW+SA+KR+LHRAT G +  LDSW + +SMP+GE V+PP+S+K T EE+  ++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 262 AVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYS 321
           A   LD  GD+P K   YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS
Sbjct: 270 A---LDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYS 329

Query: 322 SYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYAS 381
           SYSCTQGWF T C  +P LRGL+NI++IH+KK NCSEWE+I +P SVRSIV LNL+NY S
Sbjct: 330 SYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGS 389

Query: 382 GRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRI 441
           GR+PWG  +P+YLEKRGF+EAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR 
Sbjct: 390 GRHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRF 449

Query: 442 EIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMINGD 483
           E+RGG  K A+LQMDGEPWKQP+  D+STFVEIK+VP QSLMING+
Sbjct: 450 ELRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Spg007517 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 652.9 bits (1683), Expect = 2.0e-187
Identity = 311/476 (65.34%), Postives = 378/476 (79.41%), Query Frame = 0

Query: 13  IAARSSLFDS--LKGCGLSG---IRIDKHQLKTKLMMPQYLRLALRDSIRFKDPAA-GAS 72
           +A+R S  DS  ++GCGL+    + +DK +L+ +LMMP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSAIPDH 75

Query: 73  ATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQY 132
              P   VA   P AP+VVFINP SGGRHG  LK+RLQQL+SEEQVFDL +VKPHEFV+Y
Sbjct: 76  LLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRY 135

Query: 133 GLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPL 192
           GL CLE +A  GD CAK+ R ++R+MVAGGDGTVGWVLG L EL K  +  + PVG+IPL
Sbjct: 136 GLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPL 195

Query: 193 GTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKVEPPHSM 252
           GTGNDLSRSFGWGGSFPFAW+SAVKR+LHRA+ G V  LDSW + +SMP+GE V+PP+S+
Sbjct: 196 GTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSL 255

Query: 253 KLTEESDRNEAVHELDDRGDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQ 312
           K  EE   NE    LD   D P     YEGVFYNY SIGMDAQVAYGFHHLRN +PYLAQ
Sbjct: 256 KPAEE---NELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQ 315

Query: 313 GPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSI 372
           GP++NK+IYSS+ C+QGWF T C  DP LRGL+NI++IH+KK+NCS+WE+I +P +VRSI
Sbjct: 316 GPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSI 375

Query: 373 VALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAK 432
           VALNLH+Y SG +PWG  KP+YLEKRGF+EAH DDGL+EIFG KQGWH SFVM ++ISAK
Sbjct: 376 VALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAK 435

Query: 433 HIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMINGD 483
           HIAQAAA+R E+RGG  + A+LQMDGEPWKQP+S ++STFVEIK+VP QSLMIN +
Sbjct: 436 HIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of Spg007517 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 646.0 bits (1665), Expect = 2.4e-185
Identity = 299/458 (65.28%), Postives = 376/458 (82.10%), Query Frame = 0

Query: 28  LSGIRIDKHQLKTKLMMPQYLRLALRDSIRFKDPAAGASAT-APPENVAPPPPEAPLVVF 87
           L+ IR+ K +L+ ++M+PQYLR+A+RD I  KD +  AS++ APP       PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 88  INPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTR 147
           +NP+SGGR G  +K+RLQ LISEEQV+DL +VKP+EF++YGL CLE  A RGD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 148 QKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 207
           +K+R++VAGGDGTVGWVLG L EL  Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 208 KSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEKVEPPHSMKLTEES--DRNEAVHELDDR 267
           KSA+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+S+K T+E   D+N     L+  
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQN-----LEIE 274

Query: 268 GDLPTKLDCYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGW 327
           G++P   + YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYS Y C+QGW
Sbjct: 275 GEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGW 334

Query: 328 FFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKP 387
           F T C  DP LRGLKNI+ +H+KK++ SEWE++P+P SVR++VALNLH+Y SGRNPWG  
Sbjct: 335 FLTHCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNL 394

Query: 388 KPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRK 447
           K +YLEKRGF+EA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K
Sbjct: 395 KQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWK 454

Query: 448 KAYLQMDGEPWKQPISKDFSTFVEIKRVPCQSLMINGD 483
            A++QMDGEPWKQP+++D+STFV+IKRVP QSL++ GD
Sbjct: 455 DAFMQMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of Spg007517 vs. TAIR 10
Match: AT4G28130.1 (diacylglycerol kinase 6 )

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-72
Identity = 158/438 (36.07%), Postives = 236/438 (53.88%), Query Frame = 0

Query: 41  KLMMPQYLRLALRDSIRFKDPAAGASATAPPENVAPPPPEAPLVVFINPRSGGRHGQKLK 100
           K  +P Y+ L+  D     +P A  S T  PEN        P++VFIN +SGG+ G +L 
Sbjct: 20  KFFIPSYV-LSPED-----EPEAQISCTTAPEN--------PILVFINSKSGGQLGAELI 79

Query: 101 QRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTV 160
              + L++++QVFDL    P + +Q     LE L D  D+ A + R K++++VAGGDGT 
Sbjct: 80  LTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTA 139

Query: 161 GWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTG 220
           GW+LG +++L   +     P+  +PLGTGN+L  +FGWG   P   +S+V+  L +    
Sbjct: 140 GWLLGVVSDLNLSNPP---PIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINA 199

Query: 221 QVRSLDSWHVSLSMPAGEK------VEPPHSMKLTEESDRN--EAVHELDDRGDLPTKLD 280
           +   +D+W + + M   ++      ++ PHS+     SD+   E  H             
Sbjct: 200 KEMKIDNWKILMRMKHPKEGSCDITLKLPHSLPRIFPSDQENMEGYH------------- 259

Query: 281 CYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKD 340
            Y G F+NYFS+GMDAQV+Y FH  R   P   +  + N+  Y   SCTQGWFF S    
Sbjct: 260 TYRGGFWNYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-H 319

Query: 341 PNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKR 400
           P+ + +  + +I +   N  +W  + IP S+RSIV LNL +++ G NPWG P P+    R
Sbjct: 320 PSSQNIAKLAKIQICDRN-GQWNDLHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDR 379

Query: 401 GFMEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDG 460
                  DDGL+EI G +  WH   ++        +AQA  +R+E + G  K AY+++DG
Sbjct: 380 SLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDG 423

Query: 461 EPWKQPI-SKDFSTFVEI 470
           EPWKQP+ S D +  VEI
Sbjct: 440 EPWKQPLPSNDETVMVEI 423

BLAST of Spg007517 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 270.4 bits (690), Expect = 2.8e-72
Identity = 151/423 (35.70%), Postives = 226/423 (53.43%), Query Frame = 0

Query: 72  ENVAPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCL 131
           E  + P P +P++VFIN +SGG+ G +L    + L++  QVFDL    P + ++     L
Sbjct: 30  EEESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNL 89

Query: 132 EILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGND 191
           E L D  D  A+Q R+K++++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+
Sbjct: 90  ERLKD--DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNN 149

Query: 192 LSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLSMPAGEK---------VEP 251
           L  +FGWG   P   ++AV+  L +    +V  +D+WH+ + M   ++         +E 
Sbjct: 150 LPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLEL 209

Query: 252 PHSMKLTEESDRNEAVHELDDRGDLPTKLDC--YEGVFYNYFSIGMDAQVAYGFHHLRNK 311
           PHS+          A H +    +L  K  C  + G F+NYFS+GMDAQ++Y FH  R  
Sbjct: 210 PHSL---------HAFHRVSPTDEL-NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKL 269

Query: 312 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 371
            P   +  + N+  Y    CTQGWF  S    P  R +  + ++ +   N  +W+ + IP
Sbjct: 270 HPEKFKNQLVNQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIP 329

Query: 372 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFMEAHADDGLLEIFGLKQGWHTSFVMV 431
            S+RSIV LNL +++ G NPWG P P     RG      DDGL+E+ G +  WH   ++ 
Sbjct: 330 HSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLA 389

Query: 432 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDFST-FVEIKRVPCQSLMI 483
                  +AQA  IR E   G     +++MDGEPWKQP+  D  T  VEI  +   +++ 
Sbjct: 390 PNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLA 435