Spg007515 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg007515 vs. NCBI nr
Match: XP_038879910.1 (adenine/guanine permease AZG2-like [Benincasa hispida])

HSP 1 Score: 949.1 bits (2452), Expect = 1.6e-272
Identity = 482/544 (88.60%), Postives = 514/544 (94.49%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATF 60
           MGG G G   GG  GIR+SWG+MERALN AIARS VGKYFKLEARN+CFT+ELRAGLATF
Sbjct: 1   MGGSGRGDSHGGGCGIRNSWGKMERALNGAIARSMVGKYFKLEARNTCFTKELRAGLATF 60

Query: 61  LTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVG 120
           LTMAYIITVNANIL DSGG CS+ADCSAP NGTAAPDCMFKP+ GYQNCLSKTKSDLMVG
Sbjct: 61  LTMAYIITVNANILTDSGGMCSMADCSAPANGTAAPDCMFKPNPGYQNCLSKTKSDLMVG 120

Query: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCA 180
           TILSAMIGSFAMGVLANLPLGLAP MGPNAY+AYNLVGFHGSGP++YQTALAIFLVE CA
Sbjct: 121 TILSAMIGSFAMGVLANLPLGLAPAMGPNAYLAYNLVGFHGSGPIKYQTALAIFLVEACA 180

Query: 181 FIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTAC 240
           FIAVSALG+R+KLAK IPN VR+A AAGIGLFIAFVGLQAHQGLGL+GPDSATLVTLTAC
Sbjct: 181 FIAVSALGIRAKLAKLIPNSVRYACAAGIGLFIAFVGLQAHQGLGLIGPDSATLVTLTAC 240

Query: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAV 300
           SRTNPETGEC+GGKMQSPTFWLGSIGFVIMAYGLMK+FKGSMIYGIVFVTFVSWFRGTAV
Sbjct: 241 SRTNPETGECLGGKMQSPTFWLGSIGFVIMAYGLMKDFKGSMIYGIVFVTFVSWFRGTAV 300

Query: 301 TYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGT 360
           TYFPHSPLGD+KYNYFKKVVDFHKI+TTAGVVSFNGFN +EVWVAL TLFYID+LATTGT
Sbjct: 301 TYFPHSPLGDQKYNYFKKVVDFHKIETTAGVVSFNGFNRTEVWVALGTLFYIDVLATTGT 360

Query: 361 LYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGI 420
           L+TMAEIGGFVN+RG FEGEYMAYIVDG STVVAA+LGVSPVATYVESSAG+REGGRTGI
Sbjct: 361 LFTMAEIGGFVNERGNFEGEYMAYIVDGCSTVVAAMLGVSPVATYVESSAGIREGGRTGI 420

Query: 421 TAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480
           TAVVVSFCFMMSLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKE+DWGNVKESAPAF+TM
Sbjct: 421 TAVVVSFCFMMSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEVDWGNVKESAPAFVTM 480

Query: 481 VLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAEL 540
           VLMPLTYSIANGIVGGIG+YVA+SLYD+V+RLMNWL K RRVV +EQNQVSA    N EL
Sbjct: 481 VLMPLTYSIANGIVGGIGVYVAMSLYDNVMRLMNWLMKMRRVVAKEQNQVSAN-VVNTEL 540

Query: 541 TTVV 545
           T+VV
Sbjct: 541 TSVV 543

BLAST of Spg007515 vs. NCBI nr
Match: XP_038880324.1 (adenine/guanine permease AZG2-like [Benincasa hispida])

HSP 1 Score: 946.8 bits (2446), Expect = 8.1e-272
Identity = 475/532 (89.29%), Postives = 508/532 (95.49%), Query Frame = 0

Query: 11  GGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVN 70
           G  GGIR+SWG+MERALN+AIARS VGKYFKLEARN+CFT+ELRAGLATFLTMAYIITVN
Sbjct: 3   GSGGGIRNSWGKMERALNDAIARSMVGKYFKLEARNTCFTKELRAGLATFLTMAYIITVN 62

Query: 71  ANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSF 130
           ANIL DSGG CS+ADCSAP NGTAAPDCMFKP+ GYQNCLSKTKSDLMVGTILSAMIGSF
Sbjct: 63  ANILTDSGGMCSMADCSAPANGTAAPDCMFKPNPGYQNCLSKTKSDLMVGTILSAMIGSF 122

Query: 131 AMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLR 190
           AMGVLANLPLGLAP MGPNAY+AYNLVGFHGSGP++YQTALAIFLVE CAFIAVSALG+R
Sbjct: 123 AMGVLANLPLGLAPAMGPNAYLAYNLVGFHGSGPIKYQTALAIFLVEACAFIAVSALGIR 182

Query: 191 SKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTNPETGEC 250
           +KLAK IPN VR+A AAGIGLFIAFVGLQAHQGLGL+GPDSATLVTLTACSRTNPETGEC
Sbjct: 183 AKLAKLIPNSVRYACAAGIGLFIAFVGLQAHQGLGLIGPDSATLVTLTACSRTNPETGEC 242

Query: 251 IGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGD 310
           +GGKMQSPTFWLGSIGFVIMAYGLMK+FKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGD
Sbjct: 243 LGGKMQSPTFWLGSIGFVIMAYGLMKDFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGD 302

Query: 311 EKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGTLYTMAEIGGF 370
           +KYNYFKKVVDFHKI+TTAGVVSFNGFN +EVWVAL TLFYID+LATTGTL+TMAEIGGF
Sbjct: 303 QKYNYFKKVVDFHKIETTAGVVSFNGFNRTEVWVALGTLFYIDVLATTGTLFTMAEIGGF 362

Query: 371 VNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFM 430
           VN+RG FEGEYMAYIVDG STVVAA+LGVSPVATYVESSAG+REGGRTGITAVVVSFCFM
Sbjct: 363 VNERGNFEGEYMAYIVDGCSTVVAAMLGVSPVATYVESSAGIREGGRTGITAVVVSFCFM 422

Query: 431 MSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIA 490
           MSLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKE+DWGNVKESAPAF+TMVLMPLTYSIA
Sbjct: 423 MSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEVDWGNVKESAPAFVTMVLMPLTYSIA 482

Query: 491 NGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAELTT 543
           NGIVGGIG+YVA+SLYD+V+RLMNWL K RRVV +EQNQVSA    N ELT+
Sbjct: 483 NGIVGGIGVYVAMSLYDNVMRLMNWLMKMRRVVAKEQNQVSAN-VVNTELTS 533

BLAST of Spg007515 vs. NCBI nr
Match: XP_023002350.1 (adenine/guanine permease AZG2 [Cucurbita maxima])

HSP 1 Score: 944.5 bits (2440), Expect = 4.0e-271
Identity = 484/544 (88.97%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATF 60
           MGGGGDG GS G GG R+    +ERALNEAIARSFVGKYFKLEARNSCFT+ELRAGLATF
Sbjct: 1   MGGGGDGGGSSGVGGFRNPLRNIERALNEAIARSFVGKYFKLEARNSCFTKELRAGLATF 60

Query: 61  LTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVG 120
           LTMAYIITVNANILADSGGTCS+ADCS PV+GTAAPDCMFKP+ GYQNCL+  KSDLMVG
Sbjct: 61  LTMAYIITVNANILADSGGTCSMADCSVPVDGTAAPDCMFKPNPGYQNCLATAKSDLMVG 120

Query: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCA 180
           TILSAMIGSFAMGVLANLPLGLAPGMGPNAY+AYN+VGFHGSGP+ Y+TALAI LVEGCA
Sbjct: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYLAYNIVGFHGSGPIPYRTALAILLVEGCA 180

Query: 181 FIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTAC 240
           FIAVSALGLRSKLAK IP  VRFA AAGIGLFIAFVGLQ +QGLGLVGPD ATLVTLTAC
Sbjct: 181 FIAVSALGLRSKLAKLIPKSVRFACAAGIGLFIAFVGLQVNQGLGLVGPDPATLVTLTAC 240

Query: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAV 300
           SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKE KGSMIYGIVFVTFVSWFRGTAV
Sbjct: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKELKGSMIYGIVFVTFVSWFRGTAV 300

Query: 301 TYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGT 360
           TYFPHSPLGDEK+NYFKKVVDFHKIQTTAGV+SFNGFN S VWVALATLFYID+LATTGT
Sbjct: 301 TYFPHSPLGDEKFNYFKKVVDFHKIQTTAGVLSFNGFNRSPVWVALATLFYIDVLATTGT 360

Query: 361 LYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGI 420
           LYT+AEIGGFVN+RG FEGEYMAYIVDG STVVAALLGVSP+ATYVESSAG+REGGRTG+
Sbjct: 361 LYTLAEIGGFVNERGNFEGEYMAYIVDGGSTVVAALLGVSPIATYVESSAGIREGGRTGL 420

Query: 421 TAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480
           TAVVVSFCFM+SLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM
Sbjct: 421 TAVVVSFCFMLSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480

Query: 481 VLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAEL 540
           +LMPLTYSIANGIVGGIGLYVALSLYD V+R+M WLGK +RVV +EQNQVSA +AANAEL
Sbjct: 481 ILMPLTYSIANGIVGGIGLYVALSLYDQVVRVMKWLGKMKRVVAKEQNQVSA-SAANAEL 540

Query: 541 TTVV 545
            +VV
Sbjct: 541 VSVV 543

BLAST of Spg007515 vs. NCBI nr
Match: KAG6587426.1 (Adenine/guanine permease AZG2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021410.1 Adenine/guanine permease AZG2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 940.3 bits (2429), Expect = 7.6e-270
Identity = 482/544 (88.60%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATF 60
           MGGGGDG GS G GG R+    +ERALNEAIARSFVGKYFKLEARNSCFT+ELRAGLATF
Sbjct: 1   MGGGGDGGGSSGVGGFRNPLRNIERALNEAIARSFVGKYFKLEARNSCFTKELRAGLATF 60

Query: 61  LTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVG 120
           LTMAYIITVNANILADSGGTCS+ADCS PVNGTA+PDCMFKP+ GYQNCL+ TKSDLMVG
Sbjct: 61  LTMAYIITVNANILADSGGTCSMADCSVPVNGTASPDCMFKPNPGYQNCLATTKSDLMVG 120

Query: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCA 180
           TILSAMIGSFAMGVLANLPLGLAPGMGPNAY+AYNLVGFHGSGP+ Y+TALAI LVEGCA
Sbjct: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYLAYNLVGFHGSGPIPYRTALAILLVEGCA 180

Query: 181 FIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTAC 240
           FIAVSALGLRSKLAK IP  VRFA AAGIGLFIAFVGLQ +QGLGLVGPD ATLVTLTAC
Sbjct: 181 FIAVSALGLRSKLAKLIPKSVRFACAAGIGLFIAFVGLQVNQGLGLVGPDPATLVTLTAC 240

Query: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAV 300
           SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKE KGSMIYGIVFVT VSWFRGTAV
Sbjct: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKELKGSMIYGIVFVTLVSWFRGTAV 300

Query: 301 TYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGT 360
           TYFPH+PLG+EK+NYFKKVVDFHKIQTTAGV+SFNGFN + VWVALATLFYID+LATTGT
Sbjct: 301 TYFPHTPLGNEKFNYFKKVVDFHKIQTTAGVLSFNGFNRTPVWVALATLFYIDVLATTGT 360

Query: 361 LYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGI 420
           LYT+AEIGGFVN+RG FEGEYMAYIVDG STVVAALLGVSPVATYVESSAG+REGGRTG+
Sbjct: 361 LYTLAEIGGFVNERGNFEGEYMAYIVDGGSTVVAALLGVSPVATYVESSAGIREGGRTGL 420

Query: 421 TAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480
           TAVVVSFCFMMSLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKEI+WGNVKESAPAFITM
Sbjct: 421 TAVVVSFCFMMSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEIEWGNVKESAPAFITM 480

Query: 481 VLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAEL 540
           +LMPLTYSIANGIVGGIG+YVALSLYD V+R+M WLGK +RVV +EQNQVSA +AANAEL
Sbjct: 481 ILMPLTYSIANGIVGGIGVYVALSLYDQVVRVMKWLGKMKRVVAKEQNQVSA-SAANAEL 540

Query: 541 TTVV 545
            +VV
Sbjct: 541 VSVV 543

BLAST of Spg007515 vs. NCBI nr
Match: XP_022925638.1 (adenine/guanine permease AZG2 [Cucurbita moschata])

HSP 1 Score: 938.7 bits (2425), Expect = 2.2e-269
Identity = 481/544 (88.42%), Postives = 512/544 (94.12%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATF 60
           MGGGGDG GS G GG R+    +ERALNEAIARSFVGKYFKLEARNSCFT+ELRAGLATF
Sbjct: 1   MGGGGDGGGSSGVGGFRNPLRNIERALNEAIARSFVGKYFKLEARNSCFTKELRAGLATF 60

Query: 61  LTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVG 120
           LTMAYIITVNANILADSGGTCS+ADCS PVNG A+PDCMFKP+ GYQNCL+ TKSDLMVG
Sbjct: 61  LTMAYIITVNANILADSGGTCSMADCSVPVNGNASPDCMFKPNPGYQNCLATTKSDLMVG 120

Query: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCA 180
           TILSAMIGSFAMGVLANLPLGLAPGMGPNAY+AYNLVGFHGSGP+ Y+TALAI LVEGCA
Sbjct: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYLAYNLVGFHGSGPIPYRTALAILLVEGCA 180

Query: 181 FIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTAC 240
           F+AVSALGLRSKLAK IP  VRFA AAGIGLFIAFVGLQ +QGLGLVGPD ATLVTLTAC
Sbjct: 181 FVAVSALGLRSKLAKLIPKSVRFACAAGIGLFIAFVGLQVNQGLGLVGPDPATLVTLTAC 240

Query: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAV 300
           SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKE KGSMIYGIVFVT VSWFRGTAV
Sbjct: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKELKGSMIYGIVFVTLVSWFRGTAV 300

Query: 301 TYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGT 360
           TYFPHSPLGDEK+NYF+KVVDFHKIQTTAGV+SFNGFN + VWVALATLFYID+LATTGT
Sbjct: 301 TYFPHSPLGDEKFNYFQKVVDFHKIQTTAGVLSFNGFNRTPVWVALATLFYIDVLATTGT 360

Query: 361 LYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGI 420
           LYT+AEIGGFVN+RG FEGEYMAYIVDG STVVAALLGVSPVATYVESSAG+REGGRTG+
Sbjct: 361 LYTLAEIGGFVNERGNFEGEYMAYIVDGGSTVVAALLGVSPVATYVESSAGIREGGRTGL 420

Query: 421 TAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480
           TAVVVSFCFMMSLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKEI+WGNVKESAPAFITM
Sbjct: 421 TAVVVSFCFMMSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEIEWGNVKESAPAFITM 480

Query: 481 VLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAEL 540
           +LMPLTYSIANGIVGGIG+YVALSLYD V+R+M WLGK +RVV +EQNQVSA +AANAEL
Sbjct: 481 ILMPLTYSIANGIVGGIGVYVALSLYDQVVRVMKWLGKMKRVVSKEQNQVSA-SAANAEL 540

Query: 541 TTVV 545
            +VV
Sbjct: 541 VSVV 543

BLAST of Spg007515 vs. ExPASy Swiss-Prot
Match: Q84MA8 (Adenine/guanine permease AZG2 OS=Arabidopsis thaliana OX=3702 GN=AZG2 PE=2 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.4e-197
Identity = 340/517 (65.76%), Postives = 417/517 (80.66%), Query Frame = 0

Query: 19  SWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVNANILADSG 78
           SW  M++ LN+ +++SF+G++FKLEAR + FT ELRA  ATFLTMAYIITVNANILADSG
Sbjct: 10  SWSDMKKHLNDTVSKSFIGRFFKLEARKTTFTTELRAATATFLTMAYIITVNANILADSG 69

Query: 79  GTCSIADCSAPVNGT-AAPDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSFAMGVLAN 138
            TCSI DCS   + +   P+C+   + GY+ C+S+ K DL+V T LSAM+GS AMG+LAN
Sbjct: 70  ATCSINDCSTVASSSPPGPECVLGSNPGYEQCISRVKKDLVVATSLSAMVGSLAMGLLAN 129

Query: 139 LPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLRSKLAKFI 198
           LP GLAPGMG NAY+AYN+VGF GSG + Y TA+AI L+EGCAF+AVSALGLR KLA+ I
Sbjct: 130 LPFGLAPGMGANAYIAYNVVGFRGSGSISYHTAMAIVLLEGCAFLAVSALGLRGKLARLI 189

Query: 199 PNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTNPETGECIGGKMQS 258
           P  VR A A GIG+FIAFVGLQ +QG+GLVGPD +TLVTLTAC+ T+P TG C+GGKM+S
Sbjct: 190 PQTVRLACAVGIGMFIAFVGLQMNQGIGLVGPDKSTLVTLTACAETDPVTGACLGGKMKS 249

Query: 259 PTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGDEKYNYFK 318
           PTFWL  +GF+I ++GLMK  KGSMIYGIVFVT +SW RGT VT FPH+PLGD  YNYF 
Sbjct: 250 PTFWLAVVGFLITSFGLMKNVKGSMIYGIVFVTAISWIRGTQVTIFPHTPLGDSNYNYFT 309

Query: 319 KVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGTLYTMAEIGGFVNDRGTF 378
           K+VDFHKIQ+T G +SF  F  SEVWVA ATLFY+D+L TTG LYTMAEIGGFV D G F
Sbjct: 310 KIVDFHKIQSTLGAISFTEFRKSEVWVAFATLFYVDLLGTTGVLYTMAEIGGFVED-GKF 369

Query: 379 EGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFMMSLFFTP 438
           EGEY AY+VD  S+VV + LGV+  AT+VESSAG++EGG+TG+TAV+V   F+ S+FFTP
Sbjct: 370 EGEYAAYLVDAGSSVVGSALGVTTTATFVESSAGLKEGGKTGLTAVIVGLYFLASMFFTP 429

Query: 439 VLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIANGIVGGI 498
           ++++VP WA+GPSLVMVGVMMM VVK+I WG  KE+  AF+T++LMPLTYSIANGI+ GI
Sbjct: 430 LVTNVPRWAVGPSLVMVGVMMMGVVKDIRWGETKEAVTAFVTILLMPLTYSIANGIIAGI 489

Query: 499 GLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATA 535
           G+Y+ALS+YD VL +  WL   R+ V+RE NQVS+ A
Sbjct: 490 GIYLALSMYDVVLGVAKWLNGVRKRVMREHNQVSSVA 525

BLAST of Spg007515 vs. ExPASy Swiss-Prot
Match: Q9SRK7 (Adenine/guanine permease AZG1 OS=Arabidopsis thaliana OX=3702 GN=AZG1 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-156
Identity = 288/555 (51.89%), Postives = 381/555 (68.65%), Query Frame = 0

Query: 27  LNEAIARSFVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVNANILADSGGTCSIAD- 86
           LN  +  S VGK FKL  RNS FT ELRAG ATFLTMAYI+ VNA+IL+DSGGTCS++D 
Sbjct: 24  LNTYVGSSRVGKRFKLAERNSTFTTELRAGTATFLTMAYILAVNASILSDSGGTCSVSDC 83

Query: 87  -------------CSAPVNGTAAPD--CMFKP-DSGYQNCLSKTKSDLMVGTILSAMIGS 146
                        C+ P      PD  C F P + GY  C+ + + DL+V T+ +++IG 
Sbjct: 84  IPLCSNPAIEPSQCTGPGLRLIQPDVSCKFNPVNPGYAACVEEIRKDLIVATVAASLIGC 143

Query: 147 FAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGL 206
             MG++ANLPL LAPGMG NAY AY +VGFHGSG + Y+TALA   +EG  F+ +SA+G 
Sbjct: 144 VIMGLMANLPLALAPGMGTNAYFAYTVVGFHGSGSISYRTALAAVFIEGLIFLFISAIGF 203

Query: 207 RSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTN----- 266
           R+KLAK +P PVR + +AGIGLF+AF+GLQ +QG+GLVG   +TLVTL AC  ++     
Sbjct: 204 RAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLVGYSPSTLVTLAACPASSRISLA 263

Query: 267 ----------------PETGE--CIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGI 326
                             +G+  CI G+M+SPTFWLG +GFVI+AY L+K  KG+MIYGI
Sbjct: 264 PVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVIIAYCLVKNVKGAMIYGI 323

Query: 327 VFVTFVSWFRGTAVTYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVAL 386
           VFVT VSWFR T VT FP++  GD  ++YFKK+VD H I+ TAG +SF+G N    W AL
Sbjct: 324 VFVTAVSWFRNTEVTAFPNTSAGDAAHDYFKKIVDVHVIKHTAGALSFSGINKGHFWEAL 383

Query: 387 ATLFYIDILATTGTLYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYV 446
            T  Y+DIL TTGTLY+MA   GFV+++G F G+Y A++ D S+ V+ +LLG SPV  ++
Sbjct: 384 VTFLYVDILDTTGTLYSMARFAGFVDEKGDFAGQYFAFMSDASAIVIGSLLGTSPVTVFI 443

Query: 447 ESSAGMREGGRTGITAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEID 506
           ESS G+REGGRTG+TA+ V+  F++++FFTP+L+S+P WA+GP L++VGVMMMK V EID
Sbjct: 444 ESSTGIREGGRTGLTAITVAVYFLLAMFFTPLLASIPAWAVGPPLILVGVMMMKSVTEID 503

Query: 507 WGNVKESAPAFITMVLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVRE 542
           W +++E+ PAF+TM+LMPLTYS+A G++GGIG YV L L+D     +  LG  +R V  E
Sbjct: 504 WEDMREAIPAFVTMILMPLTYSVAYGLIGGIGSYVVLHLWDWGEEGLVKLGFLKRKVKEE 563

BLAST of Spg007515 vs. ExPASy Swiss-Prot
Match: L7WRR4 (Efflux pump notK' OS=Aspergillus versicolor OX=46472 GN=notK' PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.4e-101
Identity = 211/489 (43.15%), Postives = 299/489 (61.15%), Query Frame = 0

Query: 28  NEAIARSFVGKYFKLEA-------RNSCFTRELRAGLATFLTMAYIITVNANILADSGGT 87
           N A+ARS VGK+F+LE        + + F  ELRAGLATF  MAYII+VNANI +D+G T
Sbjct: 11  NTAVARSAVGKWFRLEGSGHPRERKGAYFFTELRAGLATFFAMAYIISVNANITSDTGAT 70

Query: 88  CSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSFAMGVLANLPL 147
           C    C A    T   +     ++ Y  C  +   D++  T   A + SF +G+LANLP+
Sbjct: 71  CV---CPAEDLETHCNN-----NTEYLLCKQEVNRDIVTATAAIASVASFFLGLLANLPV 130

Query: 148 GLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLRSKLAKFIPNP 207
            LAPGMG NAY AY +VG HGSG + Y  A+    VEG  F+ ++ LG+R  LA+ IP  
Sbjct: 131 ALAPGMGLNAYFAYTVVGHHGSGLIPYSLAVTAVFVEGWIFLGLTMLGIRQWLARAIPAS 190

Query: 208 VRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTNPET-GECIG-GKMQSP 267
           ++ A  AGIGL++  +GL    GLGLV     + + L  C+    ++ G C    KM++P
Sbjct: 191 IKLATGAGIGLYLTLIGLSYSAGLGLVQGAQDSPIQLAGCASDEFDSDGLCPSYAKMRNP 250

Query: 268 TFWLGSI-GFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGDEKYNYFK 327
           T W+G   G     + +M   KG++I GI+ V+ +SW R T VTYFPH+  GD  +++FK
Sbjct: 251 TMWIGIFCGGFFTVFLMMYRVKGAVIAGILLVSIISWPRTTPVTYFPHTTEGDSMFDFFK 310

Query: 328 KVVDFHKIQTTAGVVSFN-GFNSSEVWVALATLFYIDILATTGTLYTMAEIGGFVNDR-G 387
           KVV FH IQ T     +N   N  +  +AL T  Y+DIL  TGTLY+MA+  G +++R  
Sbjct: 311 KVVTFHPIQHTLVAQDWNISSNGGQFGLALITFLYVDILDATGTLYSMAKFAGAMDERTQ 370

Query: 388 TFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFMMSLFF 447
            FEG  MAY VD     + +L G  PV  +VES AG+ EGG+TG+T+ +   CF +++FF
Sbjct: 371 DFEGSAMAYTVDAICISIGSLFGSPPVTAFVESGAGISEGGKTGLTSCMTGICFFIAVFF 430

Query: 448 TPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIANGIVG 505
            P+ +S+PPWA G +LV+VG MMM    EI+W  + ++ PAF+T+ +MP TYSIA+G++ 
Sbjct: 431 APIFASIPPWATGSTLVIVGSMMMHATLEINWRYMGDAIPAFLTISVMPFTYSIADGLIA 490

BLAST of Spg007515 vs. ExPASy Swiss-Prot
Match: O94300 (Putative xanthine/uracil permease C887.17 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC887.17 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.5e-100
Identity = 218/515 (42.33%), Postives = 300/515 (58.25%), Query Frame = 0

Query: 31  IARSFVGKYFKLEA-------RNSCFTRELRAGLATFLTMAYIITVNANILADSGGTCSI 90
           +ARS  G++F+LE        + S F+ E+ AGL TF  MAYI+ VNA IL D+GGTC  
Sbjct: 18  VARSAFGRWFRLEGCGHPRERKGSRFSLEISAGLTTFFAMAYILAVNATILVDTGGTCEC 77

Query: 91  ADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSFAMGVLANLPLGLA 150
            + +         DC    D  Y  C      DL+  T   + + SF MG+ AN+P+G+A
Sbjct: 78  TEAN-------RDDCDKLDD--YVLCKEDFHRDLVTATAAISALASFCMGLFANMPVGMA 137

Query: 151 PGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLRSKLAKFIPNPVRF 210
           PGMG NAY AY +VG++G+G V Y+ AL    VEG  F  ++ +GLR  LA+ IP  ++F
Sbjct: 138 PGMGLNAYFAYQVVGYNGTGRVSYREALLAVFVEGFIFTGLTVIGLRQWLARVIPASLKF 197

Query: 211 AGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTNPE----TGECIGGKMQSPT 270
           A  AGIGL++  +GL    GLG++G  S+ +V L  C    PE       C G ++QS  
Sbjct: 198 ATGAGIGLYLTIIGLSPSAGLGVIGHSSSDIVALGGCP---PEYLNADYSCNGHQLQSGR 257

Query: 271 FWLGSI-GFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGDEKYNYFKK 330
            W+G   G V+ A  +M +FKG+++ GI  VT  SW R + VT FPH+  GD  +++FKK
Sbjct: 258 MWVGIFCGGVLTAILMMYKFKGAVLAGIALVTITSWPRRSLVTMFPHTLTGDYNFDFFKK 317

Query: 331 VVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGTLYTMAEIGGFVNDR-GTF 390
           VV F KI        +N     +  +AL T  Y+DI+  TGTLY+MA   G V+ R   F
Sbjct: 318 VVSFRKINRILVAQQWN-VTGGQFAIALITFLYVDIMDMTGTLYSMANYAGLVDPRTQDF 377

Query: 391 EGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFMMSLFFTP 450
           EG  +AYIVD  S  + +L G SPV  ++ES +G+  GGRTGI  +VV  CF +SLFF P
Sbjct: 378 EGSAVAYIVDALSISIGSLFGCSPVTAFIESGSGISAGGRTGILGMVVGICFFISLFFAP 437

Query: 451 VLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIANGIVGGI 510
           + SS+P WA G +LV+VG MMMK    I+W  + +S PAFIT+ LMP TYSIA G++ GI
Sbjct: 438 IFSSIPVWATGSTLVLVGSMMMKSTTLINWSYLGDSIPAFITIALMPFTYSIAYGLIAGI 497

Query: 511 GLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSA 533
             Y  L+   + +  M+      R+V  + NQ  A
Sbjct: 498 ICYALLNSIIYAIDKMS----RGRLVPADYNQKEA 515

BLAST of Spg007515 vs. ExPASy Swiss-Prot
Match: Q57772 (Putative permease MJ0326 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0326 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 5.3e-56
Identity = 155/482 (32.16%), Postives = 243/482 (50.41%), Query Frame = 0

Query: 35  FVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVNANILADSGGTCSIADCSAPVNGTA 94
           FV KYF+ E   +    E  AG+ TF+TMAYII VN  IL+ +G      D  A      
Sbjct: 5   FVEKYFEFEKYGTNLKVETLAGITTFMTMAYIIFVNPQILSTAG-----MDFGA------ 64

Query: 95  APDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAY 154
                                 +MV T +++ I +  MG+ A  P  LAPGMG NAY  Y
Sbjct: 65  ----------------------VMVATCIASAIATLVMGLYARYPFALAPGMGLNAYFTY 124

Query: 155 NLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIA 214
            +    G G + ++ AL    + G  FI ++   +R+ +   IPN +++  A GIGLFIA
Sbjct: 125 GVC--LGMG-IDWRVALGAVFISGVLFIILTLTKIRTWIFNVIPNAIKYGTAVGIGLFIA 184

Query: 215 FVGLQAHQGLGLVGPDSATLVTLTACSRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGL 274
           F+GL++    G++    ATLVTL               G +  P+  L   G  + +  +
Sbjct: 185 FIGLKS---AGIIVSSKATLVTL---------------GNLMEPSTLLALFGIFLTSILV 244

Query: 275 MKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSF 334
            +   G+++ GI+  + +    G        SP  +  ++    +       T   +   
Sbjct: 245 SRNVIGAILIGIIVTSLIGMILGI-------SPFPEGIFSMPPSIA-----PTFLQLDIM 304

Query: 335 NGFNSSEVWVALATLFYIDILATTGTLYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVA 394
              N   + + LA  F++D+  T GTL  +A   G+++  G       A + D + TVV 
Sbjct: 305 GALNLGLLTIVLA-FFFVDMFDTLGTLSALASQAGYLDKDGKLPRVEKALMADATGTVVG 364

Query: 395 ALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMV 454
           +LLG S V TY+ES++G+  GGRTG  +VVV+  F++SLFF PV+ ++PP+A   +LV+V
Sbjct: 365 SLLGTSTVTTYIESASGIALGGRTGFVSVVVAMLFLLSLFFYPVVKAIPPYATAAALVIV 419

Query: 455 GVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMN 514
           G +MM+ VK ID+ +  E+ PAFIT++ +PLT+SIA G+  G   Y  L ++    + ++
Sbjct: 425 GALMMRSVKYIDFDDYTEAIPAFITLLTIPLTFSIATGLALGFITYPILKVFTGRWKEVH 419

Query: 515 WL 517
           WL
Sbjct: 485 WL 419

BLAST of Spg007515 vs. ExPASy TrEMBL
Match: A0A6J1KNP2 (adenine/guanine permease AZG2 OS=Cucurbita maxima OX=3661 GN=LOC111496212 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 484/544 (88.97%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATF 60
           MGGGGDG GS G GG R+    +ERALNEAIARSFVGKYFKLEARNSCFT+ELRAGLATF
Sbjct: 1   MGGGGDGGGSSGVGGFRNPLRNIERALNEAIARSFVGKYFKLEARNSCFTKELRAGLATF 60

Query: 61  LTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVG 120
           LTMAYIITVNANILADSGGTCS+ADCS PV+GTAAPDCMFKP+ GYQNCL+  KSDLMVG
Sbjct: 61  LTMAYIITVNANILADSGGTCSMADCSVPVDGTAAPDCMFKPNPGYQNCLATAKSDLMVG 120

Query: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCA 180
           TILSAMIGSFAMGVLANLPLGLAPGMGPNAY+AYN+VGFHGSGP+ Y+TALAI LVEGCA
Sbjct: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYLAYNIVGFHGSGPIPYRTALAILLVEGCA 180

Query: 181 FIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTAC 240
           FIAVSALGLRSKLAK IP  VRFA AAGIGLFIAFVGLQ +QGLGLVGPD ATLVTLTAC
Sbjct: 181 FIAVSALGLRSKLAKLIPKSVRFACAAGIGLFIAFVGLQVNQGLGLVGPDPATLVTLTAC 240

Query: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAV 300
           SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKE KGSMIYGIVFVTFVSWFRGTAV
Sbjct: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKELKGSMIYGIVFVTFVSWFRGTAV 300

Query: 301 TYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGT 360
           TYFPHSPLGDEK+NYFKKVVDFHKIQTTAGV+SFNGFN S VWVALATLFYID+LATTGT
Sbjct: 301 TYFPHSPLGDEKFNYFKKVVDFHKIQTTAGVLSFNGFNRSPVWVALATLFYIDVLATTGT 360

Query: 361 LYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGI 420
           LYT+AEIGGFVN+RG FEGEYMAYIVDG STVVAALLGVSP+ATYVESSAG+REGGRTG+
Sbjct: 361 LYTLAEIGGFVNERGNFEGEYMAYIVDGGSTVVAALLGVSPIATYVESSAGIREGGRTGL 420

Query: 421 TAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480
           TAVVVSFCFM+SLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM
Sbjct: 421 TAVVVSFCFMLSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480

Query: 481 VLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAEL 540
           +LMPLTYSIANGIVGGIGLYVALSLYD V+R+M WLGK +RVV +EQNQVSA +AANAEL
Sbjct: 481 ILMPLTYSIANGIVGGIGLYVALSLYDQVVRVMKWLGKMKRVVAKEQNQVSA-SAANAEL 540

Query: 541 TTVV 545
            +VV
Sbjct: 541 VSVV 543

BLAST of Spg007515 vs. ExPASy TrEMBL
Match: A0A6J1EFS9 (adenine/guanine permease AZG2 OS=Cucurbita moschata OX=3662 GN=LOC111432993 PE=4 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 481/544 (88.42%), Postives = 512/544 (94.12%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGGIRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATF 60
           MGGGGDG GS G GG R+    +ERALNEAIARSFVGKYFKLEARNSCFT+ELRAGLATF
Sbjct: 1   MGGGGDGGGSSGVGGFRNPLRNIERALNEAIARSFVGKYFKLEARNSCFTKELRAGLATF 60

Query: 61  LTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVG 120
           LTMAYIITVNANILADSGGTCS+ADCS PVNG A+PDCMFKP+ GYQNCL+ TKSDLMVG
Sbjct: 61  LTMAYIITVNANILADSGGTCSMADCSVPVNGNASPDCMFKPNPGYQNCLATTKSDLMVG 120

Query: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCA 180
           TILSAMIGSFAMGVLANLPLGLAPGMGPNAY+AYNLVGFHGSGP+ Y+TALAI LVEGCA
Sbjct: 121 TILSAMIGSFAMGVLANLPLGLAPGMGPNAYLAYNLVGFHGSGPIPYRTALAILLVEGCA 180

Query: 181 FIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTAC 240
           F+AVSALGLRSKLAK IP  VRFA AAGIGLFIAFVGLQ +QGLGLVGPD ATLVTLTAC
Sbjct: 181 FVAVSALGLRSKLAKLIPKSVRFACAAGIGLFIAFVGLQVNQGLGLVGPDPATLVTLTAC 240

Query: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAV 300
           SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKE KGSMIYGIVFVT VSWFRGTAV
Sbjct: 241 SRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKELKGSMIYGIVFVTLVSWFRGTAV 300

Query: 301 TYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGT 360
           TYFPHSPLGDEK+NYF+KVVDFHKIQTTAGV+SFNGFN + VWVALATLFYID+LATTGT
Sbjct: 301 TYFPHSPLGDEKFNYFQKVVDFHKIQTTAGVLSFNGFNRTPVWVALATLFYIDVLATTGT 360

Query: 361 LYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGI 420
           LYT+AEIGGFVN+RG FEGEYMAYIVDG STVVAALLGVSPVATYVESSAG+REGGRTG+
Sbjct: 361 LYTLAEIGGFVNERGNFEGEYMAYIVDGGSTVVAALLGVSPVATYVESSAGIREGGRTGL 420

Query: 421 TAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITM 480
           TAVVVSFCFMMSLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKEI+WGNVKESAPAFITM
Sbjct: 421 TAVVVSFCFMMSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEIEWGNVKESAPAFITM 480

Query: 481 VLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAEL 540
           +LMPLTYSIANGIVGGIG+YVALSLYD V+R+M WLGK +RVV +EQNQVSA +AANAEL
Sbjct: 481 ILMPLTYSIANGIVGGIGVYVALSLYDQVVRVMKWLGKMKRVVSKEQNQVSA-SAANAEL 540

Query: 541 TTVV 545
            +VV
Sbjct: 541 VSVV 543

BLAST of Spg007515 vs. ExPASy TrEMBL
Match: A0A1S3CLM4 (adenine/guanine permease AZG2 OS=Cucumis melo OX=3656 GN=LOC103502331 PE=4 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 5.5e-266
Identity = 468/549 (85.25%), Postives = 514/549 (93.62%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAGG-----IRSSWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRA 60
           MGGGG G G G +GG      R+SW +ME+ALN+AIARS VGKYFKLEARN+CFT+ELRA
Sbjct: 1   MGGGGGGGGGGDSGGSSRVVTRNSWNKMEKALNDAIARSVVGKYFKLEARNTCFTKELRA 60

Query: 61  GLATFLTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKS 120
           GLATFLTMAYIITVNANILADSGGTCS+ADCSAP NGTA PDCM KP+ GY+NCLSK KS
Sbjct: 61  GLATFLTMAYIITVNANILADSGGTCSMADCSAPANGTATPDCMLKPNPGYENCLSKIKS 120

Query: 121 DLMVGTILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFL 180
           DLMVGTILSAMIGSFAMGVLANLPLGLAP MGPNAY+AYNLVGFHGSGP++YQTALA+FL
Sbjct: 121 DLMVGTILSAMIGSFAMGVLANLPLGLAPAMGPNAYLAYNLVGFHGSGPIKYQTALAVFL 180

Query: 181 VEGCAFIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLV 240
           VE C FIAVSALG+R+KLAKFIPN VR+A AAGIGLFIAFVGLQAHQGLGL+GPDSATLV
Sbjct: 181 VEACLFIAVSALGIRAKLAKFIPNSVRYACAAGIGLFIAFVGLQAHQGLGLIGPDSATLV 240

Query: 241 TLTACSRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWF 300
           TLTACSRTNPETGEC+GGKMQS TFWLGSIGFVIMAYGLMK+ KGSMIYGIVFVT VSWF
Sbjct: 241 TLTACSRTNPETGECLGGKMQSATFWLGSIGFVIMAYGLMKDLKGSMIYGIVFVTLVSWF 300

Query: 301 RGTAVTYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDIL 360
           RGTAVTYFPH+PLGDE+YNYF+K+VDFHKI+ TAGVVSFNGFN++EVWVALATLFYID+L
Sbjct: 301 RGTAVTYFPHNPLGDERYNYFRKIVDFHKIEKTAGVVSFNGFNTTEVWVALATLFYIDVL 360

Query: 361 ATTGTLYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREG 420
           ATTGTLYTMAEIGGFVN+RGTFEGEYMAYIVDG S+VVA +LGVSP+ATYVESSAG+REG
Sbjct: 361 ATTGTLYTMAEIGGFVNERGTFEGEYMAYIVDGCSSVVATMLGVSPIATYVESSAGIREG 420

Query: 421 GRTGITAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAP 480
           GRTGITA+VVSFCFM+SLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKE++WGNVKE+ P
Sbjct: 421 GRTGITAMVVSFCFMLSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEVEWGNVKEAVP 480

Query: 481 AFITMVLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATAA 540
           AF+TMVLMPLTYSIA+GIVGGIG+YVALSLYD+VLR+M WL K ++VV REQNQVSAT A
Sbjct: 481 AFVTMVLMPLTYSIADGIVGGIGVYVALSLYDNVLRVMKWLMKMKKVVAREQNQVSAT-A 540

Query: 541 ANAELTTVV 545
           AN ELTTVV
Sbjct: 541 ANTELTTVV 548

BLAST of Spg007515 vs. ExPASy TrEMBL
Match: A0A0A0LRB5 (Purine permease OS=Cucumis sativus OX=3659 GN=Csa_1G002730 PE=4 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.3e-264
Identity = 470/555 (84.68%), Postives = 510/555 (91.89%), Query Frame = 0

Query: 1   MGGGGDGRGSGGAG-----------GIRSSWGRMERALNEAIARSFVGKYFKLEARNSCF 60
           MGGGG G  SGG             G R+SW +ME+ALN+AIARS VGKYFKLEARN+CF
Sbjct: 1   MGGGGGGGDSGGGSSSSSSHSRSRVGTRNSWNKMEKALNDAIARSVVGKYFKLEARNTCF 60

Query: 61  TRELRAGLATFLTMAYIITVNANILADSGGTCSIADCSAPVNGTAAPDCMFKPDSGYQNC 120
           T+ELRAGLATFLTMAYIITVNANIL DSGGTCS+ADCSAPVNGTA PDCM KP+ GY+NC
Sbjct: 61  TKELRAGLATFLTMAYIITVNANILTDSGGTCSMADCSAPVNGTATPDCMLKPNPGYENC 120

Query: 121 LSKTKSDLMVGTILSAMIGSFAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQT 180
           LSK KSDLMVGT+LSAMIGSFAMGVLANLPLGLAP MGPNAY+AYNLVGFHGSGP++YQT
Sbjct: 121 LSKIKSDLMVGTVLSAMIGSFAMGVLANLPLGLAPAMGPNAYLAYNLVGFHGSGPIKYQT 180

Query: 181 ALAIFLVEGCAFIAVSALGLRSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGP 240
           ALA+FLVE C FIAVSALG+R+KLAKFIPN VR+A AAGIGLFIAFVGLQAHQGLGL+GP
Sbjct: 181 ALAVFLVEACLFIAVSALGIRAKLAKFIPNSVRYACAAGIGLFIAFVGLQAHQGLGLIGP 240

Query: 241 DSATLVTLTACSRTNPETGECIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFV 300
           DSATLVTLTACSRTN ETGEC+GGKMQS TFWLGSIGFVIMAYGLMK+ KGSMIYGIVFV
Sbjct: 241 DSATLVTLTACSRTNLETGECLGGKMQSATFWLGSIGFVIMAYGLMKDLKGSMIYGIVFV 300

Query: 301 TFVSWFRGTAVTYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVALATL 360
           T VSWFRGTAVTYFPHSPLGDE+YNYF+KVVDFHKI+ TAGVVSFNGFN++EVWVALATL
Sbjct: 301 TLVSWFRGTAVTYFPHSPLGDERYNYFRKVVDFHKIEKTAGVVSFNGFNTTEVWVALATL 360

Query: 361 FYIDILATTGTLYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYVESS 420
           FYID+LATTGTLYTMAEIGGFVN+RGTFEGEYMAYIVDG S+VVA LLGVSP+ATYVESS
Sbjct: 361 FYIDVLATTGTLYTMAEIGGFVNERGTFEGEYMAYIVDGCSSVVATLLGVSPIATYVESS 420

Query: 421 AGMREGGRTGITAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGN 480
           AG+REGGRTGITA+VVSFCFMMSLFFTP+LSSVPPWAIGPSLVMVGVMMMKVVKE++WGN
Sbjct: 421 AGIREGGRTGITAIVVSFCFMMSLFFTPLLSSVPPWAIGPSLVMVGVMMMKVVKEVEWGN 480

Query: 481 VKESAPAFITMVLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVREQNQ 540
           VKES PAF+TMVLMPLTYSIANGIVGGIG+YVALSLYD+VLRLM WL K ++VV  EQNQ
Sbjct: 481 VKESVPAFVTMVLMPLTYSIANGIVGGIGVYVALSLYDNVLRLMKWLMKMKKVVATEQNQ 540

Query: 541 VSATAAANAELTTVV 545
           VSAT AAN EL +VV
Sbjct: 541 VSAT-AANTELISVV 554

BLAST of Spg007515 vs. ExPASy TrEMBL
Match: A0A5A7UW37 (Adenine/guanine permease AZG2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00620 PE=4 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.8e-262
Identity = 455/522 (87.16%), Postives = 498/522 (95.40%), Query Frame = 0

Query: 23  MERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVNANILADSGGTCS 82
           ME+ALN+AIARS VGKYFKLEARN+CFT+ELRAGLATFLTMAYIITVNANILADSGGTCS
Sbjct: 1   MEKALNDAIARSVVGKYFKLEARNTCFTKELRAGLATFLTMAYIITVNANILADSGGTCS 60

Query: 83  IADCSAPVNGTAAPDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSFAMGVLANLPLGL 142
           +ADCSAP NGTA PDCM KP+ GY+NCLSK KSDLMVGTILSAMIGSFAMGVLANLPLGL
Sbjct: 61  MADCSAPANGTATPDCMLKPNPGYENCLSKIKSDLMVGTILSAMIGSFAMGVLANLPLGL 120

Query: 143 APGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLRSKLAKFIPNPVR 202
           AP MGPNAY+AYNLVGFHGSGP++YQTALA+FLVE C FIAVSALG+R+KLAKFIPN VR
Sbjct: 121 APAMGPNAYLAYNLVGFHGSGPIKYQTALAVFLVEACLFIAVSALGIRAKLAKFIPNSVR 180

Query: 203 FAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTNPETGECIGGKMQSPTFWL 262
           +A AAGIGLFIAFVGLQAHQGLGL+GPDSATLVTLTACSRTNPETGEC+GGKMQS TFWL
Sbjct: 181 YACAAGIGLFIAFVGLQAHQGLGLIGPDSATLVTLTACSRTNPETGECLGGKMQSATFWL 240

Query: 263 GSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGDEKYNYFKKVVDF 322
           GSIGFVIMAYGLMK+ KGSMIYGIVFVT VSWFRGTAVTYFPH+PLGDE+YNYF+K+VDF
Sbjct: 241 GSIGFVIMAYGLMKDLKGSMIYGIVFVTLVSWFRGTAVTYFPHNPLGDERYNYFRKIVDF 300

Query: 323 HKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGTLYTMAEIGGFVNDRGTFEGEYM 382
           HKI+ TAGVVSFNGFN++EVWVALATLFYID+LATTGTLYTMAEIGGFVN+RGTFEGEYM
Sbjct: 301 HKIEKTAGVVSFNGFNTTEVWVALATLFYIDVLATTGTLYTMAEIGGFVNERGTFEGEYM 360

Query: 383 AYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFMMSLFFTPVLSSV 442
           AYIVDG S+VVA +LGVSP+ATYVESSAG+REGGRTGITA+VVSFCFM+SLFFTP+LSSV
Sbjct: 361 AYIVDGCSSVVATMLGVSPIATYVESSAGIREGGRTGITAMVVSFCFMLSLFFTPLLSSV 420

Query: 443 PPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIANGIVGGIGLYVA 502
           PPWAIGPSLVMVGVMMMKVVKE++WGNVKE+ PAF+TMVLMPLTYSIA+GIVGGIG+YVA
Sbjct: 421 PPWAIGPSLVMVGVMMMKVVKEVEWGNVKEAVPAFVTMVLMPLTYSIADGIVGGIGVYVA 480

Query: 503 LSLYDHVLRLMNWLGKTRRVVVREQNQVSATAAANAELTTVV 545
           LSLYD+VLR+M WL K ++VV REQNQVSAT AAN ELTTVV
Sbjct: 481 LSLYDNVLRVMKWLMKMKKVVAREQNQVSAT-AANTELTTVV 521

BLAST of Spg007515 vs. TAIR 10
Match: AT5G50300.1 (Xanthine/uracil permease family protein )

HSP 1 Score: 690.6 bits (1781), Expect = 9.8e-199
Identity = 340/517 (65.76%), Postives = 417/517 (80.66%), Query Frame = 0

Query: 19  SWGRMERALNEAIARSFVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVNANILADSG 78
           SW  M++ LN+ +++SF+G++FKLEAR + FT ELRA  ATFLTMAYIITVNANILADSG
Sbjct: 10  SWSDMKKHLNDTVSKSFIGRFFKLEARKTTFTTELRAATATFLTMAYIITVNANILADSG 69

Query: 79  GTCSIADCSAPVNGT-AAPDCMFKPDSGYQNCLSKTKSDLMVGTILSAMIGSFAMGVLAN 138
            TCSI DCS   + +   P+C+   + GY+ C+S+ K DL+V T LSAM+GS AMG+LAN
Sbjct: 70  ATCSINDCSTVASSSPPGPECVLGSNPGYEQCISRVKKDLVVATSLSAMVGSLAMGLLAN 129

Query: 139 LPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGLRSKLAKFI 198
           LP GLAPGMG NAY+AYN+VGF GSG + Y TA+AI L+EGCAF+AVSALGLR KLA+ I
Sbjct: 130 LPFGLAPGMGANAYIAYNVVGFRGSGSISYHTAMAIVLLEGCAFLAVSALGLRGKLARLI 189

Query: 199 PNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTNPETGECIGGKMQS 258
           P  VR A A GIG+FIAFVGLQ +QG+GLVGPD +TLVTLTAC+ T+P TG C+GGKM+S
Sbjct: 190 PQTVRLACAVGIGMFIAFVGLQMNQGIGLVGPDKSTLVTLTACAETDPVTGACLGGKMKS 249

Query: 259 PTFWLGSIGFVIMAYGLMKEFKGSMIYGIVFVTFVSWFRGTAVTYFPHSPLGDEKYNYFK 318
           PTFWL  +GF+I ++GLMK  KGSMIYGIVFVT +SW RGT VT FPH+PLGD  YNYF 
Sbjct: 250 PTFWLAVVGFLITSFGLMKNVKGSMIYGIVFVTAISWIRGTQVTIFPHTPLGDSNYNYFT 309

Query: 319 KVVDFHKIQTTAGVVSFNGFNSSEVWVALATLFYIDILATTGTLYTMAEIGGFVNDRGTF 378
           K+VDFHKIQ+T G +SF  F  SEVWVA ATLFY+D+L TTG LYTMAEIGGFV D G F
Sbjct: 310 KIVDFHKIQSTLGAISFTEFRKSEVWVAFATLFYVDLLGTTGVLYTMAEIGGFVED-GKF 369

Query: 379 EGEYMAYIVDGSSTVVAALLGVSPVATYVESSAGMREGGRTGITAVVVSFCFMMSLFFTP 438
           EGEY AY+VD  S+VV + LGV+  AT+VESSAG++EGG+TG+TAV+V   F+ S+FFTP
Sbjct: 370 EGEYAAYLVDAGSSVVGSALGVTTTATFVESSAGLKEGGKTGLTAVIVGLYFLASMFFTP 429

Query: 439 VLSSVPPWAIGPSLVMVGVMMMKVVKEIDWGNVKESAPAFITMVLMPLTYSIANGIVGGI 498
           ++++VP WA+GPSLVMVGVMMM VVK+I WG  KE+  AF+T++LMPLTYSIANGI+ GI
Sbjct: 430 LVTNVPRWAVGPSLVMVGVMMMGVVKDIRWGETKEAVTAFVTILLMPLTYSIANGIIAGI 489

Query: 499 GLYVALSLYDHVLRLMNWLGKTRRVVVREQNQVSATA 535
           G+Y+ALS+YD VL +  WL   R+ V+RE NQVS+ A
Sbjct: 490 GIYLALSMYDVVLGVAKWLNGVRKRVMREHNQVSSVA 525

BLAST of Spg007515 vs. TAIR 10
Match: AT3G10960.1 (AZA-guanine resistant1 )

HSP 1 Score: 553.1 bits (1424), Expect = 2.4e-157
Identity = 288/555 (51.89%), Postives = 381/555 (68.65%), Query Frame = 0

Query: 27  LNEAIARSFVGKYFKLEARNSCFTRELRAGLATFLTMAYIITVNANILADSGGTCSIAD- 86
           LN  +  S VGK FKL  RNS FT ELRAG ATFLTMAYI+ VNA+IL+DSGGTCS++D 
Sbjct: 24  LNTYVGSSRVGKRFKLAERNSTFTTELRAGTATFLTMAYILAVNASILSDSGGTCSVSDC 83

Query: 87  -------------CSAPVNGTAAPD--CMFKP-DSGYQNCLSKTKSDLMVGTILSAMIGS 146
                        C+ P      PD  C F P + GY  C+ + + DL+V T+ +++IG 
Sbjct: 84  IPLCSNPAIEPSQCTGPGLRLIQPDVSCKFNPVNPGYAACVEEIRKDLIVATVAASLIGC 143

Query: 147 FAMGVLANLPLGLAPGMGPNAYMAYNLVGFHGSGPVRYQTALAIFLVEGCAFIAVSALGL 206
             MG++ANLPL LAPGMG NAY AY +VGFHGSG + Y+TALA   +EG  F+ +SA+G 
Sbjct: 144 VIMGLMANLPLALAPGMGTNAYFAYTVVGFHGSGSISYRTALAAVFIEGLIFLFISAIGF 203

Query: 207 RSKLAKFIPNPVRFAGAAGIGLFIAFVGLQAHQGLGLVGPDSATLVTLTACSRTN----- 266
           R+KLAK +P PVR + +AGIGLF+AF+GLQ +QG+GLVG   +TLVTL AC  ++     
Sbjct: 204 RAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLVGYSPSTLVTLAACPASSRISLA 263

Query: 267 ----------------PETGE--CIGGKMQSPTFWLGSIGFVIMAYGLMKEFKGSMIYGI 326
                             +G+  CI G+M+SPTFWLG +GFVI+AY L+K  KG+MIYGI
Sbjct: 264 PVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVIIAYCLVKNVKGAMIYGI 323

Query: 327 VFVTFVSWFRGTAVTYFPHSPLGDEKYNYFKKVVDFHKIQTTAGVVSFNGFNSSEVWVAL 386
           VFVT VSWFR T VT FP++  GD  ++YFKK+VD H I+ TAG +SF+G N    W AL
Sbjct: 324 VFVTAVSWFRNTEVTAFPNTSAGDAAHDYFKKIVDVHVIKHTAGALSFSGINKGHFWEAL 383

Query: 387 ATLFYIDILATTGTLYTMAEIGGFVNDRGTFEGEYMAYIVDGSSTVVAALLGVSPVATYV 446
            T  Y+DIL TTGTLY+MA   GFV+++G F G+Y A++ D S+ V+ +LLG SPV  ++
Sbjct: 384 VTFLYVDILDTTGTLYSMARFAGFVDEKGDFAGQYFAFMSDASAIVIGSLLGTSPVTVFI 443

Query: 447 ESSAGMREGGRTGITAVVVSFCFMMSLFFTPVLSSVPPWAIGPSLVMVGVMMMKVVKEID 506
           ESS G+REGGRTG+TA+ V+  F++++FFTP+L+S+P WA+GP L++VGVMMMK V EID
Sbjct: 444 ESSTGIREGGRTGLTAITVAVYFLLAMFFTPLLASIPAWAVGPPLILVGVMMMKSVTEID 503

Query: 507 WGNVKESAPAFITMVLMPLTYSIANGIVGGIGLYVALSLYDHVLRLMNWLGKTRRVVVRE 542
           W +++E+ PAF+TM+LMPLTYS+A G++GGIG YV L L+D     +  LG  +R V  E
Sbjct: 504 WEDMREAIPAFVTMILMPLTYSVAYGLIGGIGSYVVLHLWDWGEEGLVKLGFLKRKVKEE 563