Homology
BLAST of Spg007501 vs. NCBI nr
Match:
KAG7023113.1 (Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 323/344 (93.90%), Postives = 335/344 (97.38%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIITGAT
Sbjct: 1 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGAT 60
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 61 SGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 120
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETAAK
Sbjct: 121 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAK 180
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIINVSSV+HGWVKKDGL+FGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 181 TGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 240
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDEL+AQKLWT+TRNLIN++LSKLST
Sbjct: 301 KSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 344
BLAST of Spg007501 vs. NCBI nr
Match:
XP_022921530.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Cucurbita moschata])
HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 322/344 (93.60%), Postives = 334/344 (97.09%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIITGAT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGAT 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 SGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETA K
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETATK 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIIN+SSV+HGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINISSVVHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDEL+AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 346
BLAST of Spg007501 vs. NCBI nr
Match:
XP_022988395.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 607.8 bits (1566), Expect = 5.7e-170
Identity = 321/344 (93.31%), Postives = 335/344 (97.38%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIITGAT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGAT 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 SGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETAA+
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAQ 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIINVSSV+HGWVKKDGL+FGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDE++AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDEIEAQKLWTRTRNLINKQLSKLST 346
BLAST of Spg007501 vs. NCBI nr
Match:
XP_023516084.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 607.4 bits (1565), Expect = 7.4e-170
Identity = 322/344 (93.60%), Postives = 334/344 (97.09%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIITGAT
Sbjct: 3 MAFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGAT 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 SGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
C+HFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETAAK
Sbjct: 123 CSHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAK 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIINVSSV+HGWVKKDGL+FGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDEL+AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 346
BLAST of Spg007501 vs. NCBI nr
Match:
XP_022921531.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X2 [Cucurbita moschata])
HSP 1 Score: 597.4 bits (1539), Expect = 7.7e-167
Identity = 318/344 (92.44%), Postives = 330/344 (95.93%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIIT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIIT--- 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
GIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 -GIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETA K
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETATK 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIIN+SSV+HGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINISSVVHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDEL+AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 342
BLAST of Spg007501 vs. ExPASy Swiss-Prot
Match:
A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)
HSP 1 Score: 266.5 bits (680), Expect = 4.1e-70
Identity = 151/315 (47.94%), Postives = 202/315 (64.13%), Query Frame = 0
Query: 22 LAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKRGVRIVMP 81
+ G AG SGYGS STAE VT + LTAIITG TSGIG E ARVL RG +++
Sbjct: 7 VTGKAGKSGYGSASTAEDVTHS---IDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIA 66
Query: 82 ARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNILINNAGVF 141
AR+ K A KE I + P A I ++DLSS+ SV+ F + FLAL PLNILINNAGV
Sbjct: 67 ARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVM 126
Query: 142 SKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVIHGWVKKD 201
+ SED +E FATN++GH+LLT +LL+KM +A ++GIEGRI+N+SS+ H + +
Sbjct: 127 FCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTE 186
Query: 202 GLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPGIVKTAIIR 261
G+ F + +P+ Y+ +AY QSKLAN+LH+ +SR+LQ +TIN+VHPG++ T + R
Sbjct: 187 GIMFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR 246
Query: 262 AHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETICSALANDE 321
H G L M+ L K QGA+TTCYVAL +G +GK++ DCN T S A D
Sbjct: 247 -HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDT 306
Query: 322 LQAQKLWTQTRNLIN 337
A KLW + L++
Sbjct: 307 TLADKLWDFSIKLVD 317
BLAST of Spg007501 vs. ExPASy Swiss-Prot
Match:
A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)
HSP 1 Score: 263.8 bits (673), Expect = 2.6e-69
Identity = 150/314 (47.77%), Postives = 201/314 (64.01%), Query Frame = 0
Query: 22 LAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKRGVRIVMP 81
+ G AG SGYGS STAE VT + LTAIITG TSGIG E ARVL RG +++
Sbjct: 7 VTGKAGKSGYGSASTAEDVTHS---IDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIA 66
Query: 82 ARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNILINNAGVF 141
+R+ K A KE I + P A I ++DLSS+ SV+ F + FLAL PLNILINNAGV
Sbjct: 67 SRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVM 126
Query: 142 SKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVIHGWVKKD 201
+ SED +E FATN++GH+LLT +LL+KM +A ++GIEGRI+N+SS+ H + +
Sbjct: 127 FCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTE 186
Query: 202 GLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPGIVKTAIIR 261
G+ F + +P+ Y+ +AY QSKLAN+LH+ +SR+LQ +TIN+VHPG++ T + R
Sbjct: 187 GIMFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR 246
Query: 262 AHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETICSALANDE 321
H G L M+ L K QGA+TTCYVAL + +GK++ADCN T S A D
Sbjct: 247 -HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDT 306
Query: 322 LQAQKLWTQTRNLI 336
A KLW + L+
Sbjct: 307 TLADKLWDFSIKLV 316
BLAST of Spg007501 vs. ExPASy Swiss-Prot
Match:
A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)
HSP 1 Score: 248.8 bits (634), Expect = 8.8e-65
Identity = 137/321 (42.68%), Postives = 210/321 (65.42%), Query Frame = 0
Query: 24 GIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKRGVRIVMPAR 83
G G SG+ S+STAE+VT + LTAI+TGA+SGIG ETARVL+ RGV +VM R
Sbjct: 5 GSKGASGFSSRSTAEEVTHGVDGTG---LTAIVTGASSGIGVETARVLSLRGVHVVMAVR 64
Query: 84 DLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNILINNAGVFSK 143
+ A+VKE I K+ P A++ V E+DLSS+ SV++F + + + G PLN+LINNAG+ +
Sbjct: 65 NTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMAC 124
Query: 144 NLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVIHGWVKKDGL 203
S+D +EL FATN+LGH+LLT++LL+ M T+ ++ EGRI+N+SS H + +G+
Sbjct: 125 PFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGV 184
Query: 204 NFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPGIVKTAIIRAH 263
F ++ + ++Y+ RAY QSKL N+LHA E+++QL+ +T N++HPG + T + R
Sbjct: 185 RFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYF 244
Query: 264 KGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETICSALANDELQ 323
++ ++ +A +LK+ QGA+TTCYVAL+ Q G SG+++ D N L D
Sbjct: 245 NPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTEL 304
Query: 324 AQKLWTQTRNLINRRLSKLST 345
A+K+W + L + + + S+
Sbjct: 305 AKKVWDFSTKLTDSQSGESSS 322
BLAST of Spg007501 vs. ExPASy Swiss-Prot
Match:
Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)
HSP 1 Score: 248.1 bits (632), Expect = 1.5e-64
Identity = 135/312 (43.27%), Postives = 202/312 (64.74%), Query Frame = 0
Query: 27 GPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKRGVRIVMPARDLK 86
G SG+ STAEQVT + LTAI+TGA+SGIGAET RVLA RG ++M R++
Sbjct: 9 GVSGFSGSSTAEQVTHG---IDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMV 68
Query: 87 KAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNILINNAGVFSKNLE 146
A VK+ I K+ P A++ E+DLSSL SV++F + F + G PLNILINNAG+ + +
Sbjct: 69 AAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128
Query: 147 FSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVIHGWVKKDGLNFG 206
S+D +EL FATN++GH+LLT +LL+ M +T ++ EGRI+NV+S H + +G+ F
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFD 188
Query: 207 QMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPGIVKTAIIRAHKGF 266
++ + ++YN RAY QSKLAN+LHA ++++ L+ +T N++HPG + T + R H
Sbjct: 189 KINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR-HNSA 248
Query: 267 ITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETICSALANDELQAQK 326
+ + + +LK QGA+TTCYVAL Q +G SG++++D N + D A+K
Sbjct: 249 VNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKK 308
Query: 327 LWTQTRNLINRR 339
LW + NL+ ++
Sbjct: 309 LWDFSINLVKQK 316
BLAST of Spg007501 vs. ExPASy Swiss-Prot
Match:
P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)
HSP 1 Score: 174.5 bits (441), Expect = 2.1e-42
Identity = 110/284 (38.73%), Postives = 166/284 (58.45%), Query Frame = 0
Query: 55 IITGATSGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSL 114
+ITGA +GIG ETAR LA+RG R+ + RD+ K IQ ++ ++++V ++DLS
Sbjct: 43 VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102
Query: 115 ASVQRFCNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKM 174
S++ F FLA L+ILINNAGV + D E A N+LGH+LLT +LL ++
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162
Query: 175 IETAAKTGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEM 234
E+A R++N+SSV H K + F + YN AY SKLAN+L +E+
Sbjct: 163 KESA-----PARVVNLSSVAHHLGK---IRFHDLQGDKYYNLGFAYCHSKLANVLFTREL 222
Query: 235 SRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVAL 294
+++L+G VT AVHPGIV++ ++R F+ L+ + S LKTT +GA T+ + AL
Sbjct: 223 AKRLKGTG--VTTYAVHPGIVRSKLVR--HSFLLCLLWRLFSPFLKTTWEGAQTSLHCAL 282
Query: 295 SSQTEGKSGKFYADCNETICSALANDELQAQKLWTQTRNLINRR 339
+ E SGK+++DC +T S A + A++LW + L+ R
Sbjct: 283 AEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVSCELLGIR 314
BLAST of Spg007501 vs. ExPASy TrEMBL
Match:
A0A6J1E617 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429765 PE=3 SV=1)
HSP 1 Score: 610.1 bits (1572), Expect = 5.5e-171
Identity = 322/344 (93.60%), Postives = 334/344 (97.09%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIITGAT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGAT 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 SGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETA K
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETATK 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIIN+SSV+HGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINISSVVHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDEL+AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 346
BLAST of Spg007501 vs. ExPASy TrEMBL
Match:
A0A6J1JJF7 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485650 PE=3 SV=1)
HSP 1 Score: 607.8 bits (1566), Expect = 2.7e-170
Identity = 321/344 (93.31%), Postives = 335/344 (97.38%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIITGAT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGAT 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 SGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETAA+
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAQ 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIINVSSV+HGWVKKDGL+FGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDE++AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDEIEAQKLWTRTRNLINKQLSKLST 346
BLAST of Spg007501 vs. ExPASy TrEMBL
Match:
A0A6J1E1M6 (short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429765 PE=3 SV=1)
HSP 1 Score: 597.4 bits (1539), Expect = 3.7e-167
Identity = 318/344 (92.44%), Postives = 330/344 (95.93%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIIT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIIT--- 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
GIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 -GIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETA K
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETATK 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIIN+SSV+HGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINISSVVHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDEL+AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 342
BLAST of Spg007501 vs. ExPASy TrEMBL
Match:
A0A1S3CLP7 (short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502388 PE=3 SV=1)
HSP 1 Score: 596.3 bits (1536), Expect = 8.3e-167
Identity = 318/342 (92.98%), Postives = 329/342 (96.20%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGI GPSGYGSKSTAEQV+ SS SS+SQLTAIITGAT
Sbjct: 20 MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFSSSSSSSQLTAIITGAT 79
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
SGIGAETARVLAKRGV+IVM ARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQ F
Sbjct: 80 SGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSF 139
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CN FL+LG PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTE LLEKMIETAAK
Sbjct: 140 CNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAK 199
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIINVSSV+HGWVKKDGL+F QMLNPN YNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 200 TGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKELSRQLQG 259
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 260 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 319
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKL 343
KSGKFYADCNET CS+LANDEL+AQKLWTQTRNLINRRLSKL
Sbjct: 320 KSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKL 361
BLAST of Spg007501 vs. ExPASy TrEMBL
Match:
A0A6J1JH35 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485650 PE=3 SV=1)
HSP 1 Score: 595.1 bits (1533), Expect = 1.8e-166
Identity = 317/344 (92.15%), Postives = 331/344 (96.22%), Query Frame = 0
Query: 1 MLFSVSIILSLAKLMKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGAT 60
M+FSVSIILSL KLMK+TLRYLAGIAGPSGYGSKSTAEQVTQ SSFSSTSQLTAIIT
Sbjct: 3 MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIIT--- 62
Query: 61 SGIGAETARVLAKRGVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRF 120
GIGAETARVLAKRGVRIVMPARD+KKAAQVKEAIQ+ESPEAEIIV EIDLSSLASVQ F
Sbjct: 63 -GIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSF 122
Query: 121 CNHFLALGFPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAK 180
CNHFLAL PLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LTE LLEKMIETAA+
Sbjct: 123 CNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAQ 182
Query: 181 TGIEGRIINVSSVIHGWVKKDGLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQG 240
TGIEGRIINVSSV+HGWVKKDGL+FGQMLNPNNYNGTRAYAQSKLANILHAKE+SRQLQG
Sbjct: 183 TGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQG 242
Query: 241 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 300
RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG
Sbjct: 243 RNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEG 302
Query: 301 KSGKFYADCNETICSALANDELQAQKLWTQTRNLINRRLSKLST 345
KSGKFYADCNET CS+LANDE++AQKLWT+TRNLIN++LSKLST
Sbjct: 303 KSGKFYADCNETNCSSLANDEIEAQKLWTRTRNLINKQLSKLST 342
BLAST of Spg007501 vs. TAIR 10
Match:
AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 452.6 bits (1163), Expect = 2.8e-127
Identity = 242/326 (74.23%), Postives = 280/326 (85.89%), Query Frame = 0
Query: 15 MKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKR 74
MK TLRYLAGIAGP+G+GS+STAEQVTQ+ SF S LTAIITG TSGIGAETARVLAKR
Sbjct: 1 MKGTLRYLAGIAGPNGFGSRSTAEQVTQH-SFFPCSHLTAIITGGTSGIGAETARVLAKR 60
Query: 75 GVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNIL 134
GVR+VM RD+KKA VKE I +E+PEA+II+FEIDLSSL+SV RFC+ FL+ PLNIL
Sbjct: 61 GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120
Query: 135 INNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVI 194
INNAGVFS NLEFSE+K+ELTFATN+LGHYLLTEML+EKMI+TA K+GIEGRIIN+SSVI
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180
Query: 195 HGWVKKDGLNFGQMLNP-NNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPG 254
H WVK D +F ++L+P + YNGTRAYAQSKLA ILHAK +S+QL+ RNA VTINAVHPG
Sbjct: 181 HNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPG 240
Query: 255 IVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETI 314
IVKT IIRAHKG TDSLF +ASKLLK+ SQGA+TTCYVALS++T+G SGK++ADCNET
Sbjct: 241 IVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNETN 300
Query: 315 CSALANDELQAQKLWTQTRNLINRRL 340
CS LANDE A KL TQ+R LI+ L
Sbjct: 301 CSDLANDEYVALKLCTQSRALIHDHL 325
BLAST of Spg007501 vs. TAIR 10
Match:
AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 383.6 bits (984), Expect = 1.6e-106
Identity = 205/271 (75.65%), Postives = 235/271 (86.72%), Query Frame = 0
Query: 15 MKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKR 74
MK TLRYLAGIAGP+G+GS+STAEQVTQ+ SF S LTAIITG TSGIGAETARVLAKR
Sbjct: 1 MKGTLRYLAGIAGPNGFGSRSTAEQVTQH-SFFPCSHLTAIITGGTSGIGAETARVLAKR 60
Query: 75 GVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNIL 134
GVR+VM RD+KKA VKE I +E+PEA+II+FEIDLSSL+SV RFC+ FL+ PLNIL
Sbjct: 61 GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120
Query: 135 INNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVI 194
INNAGVFS NLEFSE+K+ELTFATN+LGHYLLTEML+EKMI+TA K+GIEGRIIN+SSVI
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180
Query: 195 HGWVKKDGLNFGQMLNP-NNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPG 254
H WVK D +F ++L+P + YNGTRAYAQSKLA ILHAK +S+QL+ RNA VTINAVHPG
Sbjct: 181 HNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPG 240
Query: 255 IVKTAIIRAHKGFITDSLFFMASKLLKTTSQ 285
IVKT IIRAHKG TDSLF +ASKLLK+ SQ
Sbjct: 241 IVKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270
BLAST of Spg007501 vs. TAIR 10
Match:
AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 340.1 bits (871), Expect = 2.1e-93
Identity = 184/324 (56.79%), Postives = 236/324 (72.84%), Query Frame = 0
Query: 15 MKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKR 74
M ET+++L G GPSG+GS+STA+ VT NS S LTAIITGATSGIGAETARVLAKR
Sbjct: 1 MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRS---LTAIITGATSGIGAETARVLAKR 60
Query: 75 GVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNIL 134
G R+V+PAR +K A + K I E P+AEIIV +DLSSL SV+RF + F +L PLNIL
Sbjct: 61 GARLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120
Query: 135 INNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVI 194
INNAG ++ SED VE+TFATNYLGH+LLT++LL+KMIETAA+TG++GRI+NV+SV+
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180
Query: 195 HGWVKKDGLNFGQMLNPN--NYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHP 254
H W D L + ++ N NY+ TRAYA SKLAN+LH E+SR L +A VT N VHP
Sbjct: 181 HSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHP 240
Query: 255 GIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNET 314
GIVKT + R +G +TD +FF+ SKLLK+ Q A+TTCYVA S + GK+++DCNE
Sbjct: 241 GIVKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEA 300
Query: 315 ICSALANDELQAQKLWTQTRNLIN 337
S + L+AQ+LWT + L++
Sbjct: 301 RSSKSGSCNLKAQRLWTASDLLVS 321
BLAST of Spg007501 vs. TAIR 10
Match:
AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 333.2 bits (853), Expect = 2.5e-91
Identity = 175/325 (53.85%), Postives = 234/325 (72.00%), Query Frame = 0
Query: 15 MKETLRYLAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKR 74
M ET +YL G AG SG+GSKSTAE+VT+N S +TA+ITGATSGIGAETARVLAKR
Sbjct: 1 MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRS---ITAVITGATSGIGAETARVLAKR 60
Query: 75 GVRIVMPARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNIL 134
G R++ PAR++K A + KE I E PE EI+V ++DLSS+ASV+ F F +L PLN+L
Sbjct: 61 GARLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120
Query: 135 INNAGVFSKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVI 194
INNAG + SED +E+TFATNYLGH+LLT +LL KMI+TA +TG++GRI+NV+S I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180
Query: 195 HGWVKKDGLNFGQMLNPN--NYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHP 254
HGW D + + ++++ ++ TRAYA SKLAN+LH KE+S +LQ A VT+N VHP
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHP 240
Query: 255 GIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNET 314
G+V+T + R +G +TD +FF+ASKL+KT Q A+TTCYVA + + SGK++ DCNET
Sbjct: 241 GVVRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNET 300
Query: 315 ICSALANDELQAQKLWTQTRNLINR 338
S L + +A KLW + L+ +
Sbjct: 301 TPSGLGTNSSEATKLWAASEILVTQ 322
BLAST of Spg007501 vs. TAIR 10
Match:
AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 280.4 bits (716), Expect = 1.9e-75
Identity = 155/315 (49.21%), Postives = 208/315 (66.03%), Query Frame = 0
Query: 22 LAGIAGPSGYGSKSTAEQVTQNSSFSSTSQLTAIITGATSGIGAETARVLAKRGVRIVMP 81
+ G GPSG+GS STAE+VTQ + LTAIITG T GIG ETARVL+KRG +V+
Sbjct: 7 ITGRRGPSGFGSASTAEEVTQG---IDATNLTAIITGGTGGIGMETARVLSKRGAHVVIG 66
Query: 82 ARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQRFCNHFLALGFPLNILINNAGVF 141
AR++ A K I +++ A + + ++DLSS+ S++ F F AL PLN+LINNAGV
Sbjct: 67 ARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVM 126
Query: 142 SKNLEFSEDKVELTFATNYLGHYLLTEMLLEKMIETAAKTGIEGRIINVSSVIHGWVKKD 201
+ SED +EL FATN++GH+LLT +LL+ M TA +G+EGRI+NVSSV H + ++
Sbjct: 127 FCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQE 186
Query: 202 GLNFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQGRNARVTINAVHPGIVKTAIIR 261
G+ F + + +Y+ RAY QSKLANILHA E+SRQLQ +T N+VHPG++ T + +
Sbjct: 187 GIQFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQ 246
Query: 262 AHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETICSALANDE 321
H + L F + L K QGA+TTCYVAL +G +GK++ADCNE S LA DE
Sbjct: 247 -HTALLMRFLKFFSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDE 306
Query: 322 LQAQKLWTQTRNLIN 337
AQKLW + LIN
Sbjct: 307 TLAQKLWDFSVKLIN 317
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7023113.1 | 1.1e-170 | 93.90 | Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma... | [more] |
XP_022921530.1 | 1.1e-170 | 93.60 | short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Cucurbita moschata] | [more] |
XP_022988395.1 | 5.7e-170 | 93.31 | short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita maxim... | [more] |
XP_023516084.1 | 7.4e-170 | 93.60 | short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo... | [more] |
XP_022921531.1 | 7.7e-167 | 92.44 | short-chain dehydrogenase TIC 32, chloroplastic isoform X2 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
A0A078ISJ6 | 4.1e-70 | 47.94 | Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... | [more] |
A0A078IS66 | 2.6e-69 | 47.77 | Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... | [more] |
A2RVM0 | 8.8e-65 | 42.68 | Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... | [more] |
Q6RVV4 | 1.5e-64 | 43.27 | Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... | [more] |
P59837 | 2.1e-42 | 38.73 | Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1E617 | 5.5e-171 | 93.60 | short-chain dehydrogenase TIC 32, chloroplastic isoform X1 OS=Cucurbita moschata... | [more] |
A0A6J1JJF7 | 2.7e-170 | 93.31 | short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... | [more] |
A0A6J1E1M6 | 3.7e-167 | 92.44 | short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Cucurbita moschata... | [more] |
A0A1S3CLP7 | 8.3e-167 | 92.98 | short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10... | [more] |
A0A6J1JH35 | 1.8e-166 | 92.15 | short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... | [more] |
Match Name | E-value | Identity | Description | |
AT5G50130.1 | 2.8e-127 | 74.23 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT5G50130.2 | 1.6e-106 | 75.65 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT1G64590.1 | 2.1e-93 | 56.79 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT4G24050.1 | 2.5e-91 | 53.85 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT5G02540.1 | 1.9e-75 | 49.21 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |