Spg007450 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg007450
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold2: 82450 .. 90977 (+)
RNA-Seq ExpressionSpg007450
SyntenySpg007450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGATGCGATCATCCAATCCCAATCGTTTCAATCGTCTATTCACCACGGGTCTGGGCATCGGGTCTCCATCTCTCTTCCCTCTTTCTCTCCCATTTGCACTCTCCGCTCATGGCGCAGGTTTCTCAGCTGCCTTGCGACGGCGATGGCATTTGCATGCTCTGCCAGGCCAAGCCTTCCGACTTGGAGACGATCACTTGCAAAACTTGCGTCACTCCGTGGCACGTCGGTTGTCTCTCAAATCCCCCTGAAACTCTGGCATCTACCCTTCAATGGGACTGTCCCGACTGTTCCCCCCCTCGCGAAGATGCTCCCCTTCCTCCACCCGGCAACTCTCCACTCCCCACTGCGCCTTCCAGTGACCTGGTTGCTGCGATTCGAGCCATTGAGGCCGATGCCTCCCTTACTGACCGCGAGAAGGCAAAGAAGCGCCAGGAGCTTCTTAGCGGAAAGTCTCAGTCCGAGAAGGATGATCATGATGCCAACAAGGAAAAGAGAAAAAGAGGGGACGATGTTTTGGATTTGTTCGACGAGAGATTGAACTGCTCCTTCTGCATGCAGTTACCAGAAAGGCCAGTCACTGTAAGTTCCCTATTGCATCGTTCTTTATCTTCTTTGACATGTCAGACCACCTGCTCCTTTCTTCTTGCCCTTGCATTACTTGCAAGACTCTTTAACCGTGTCTTTAGCTGTCTGTGTCATCTCACAAATGACATTTCATCTTCCATCTCATCGGTTTTTATTTTCGATTCACTTGTTTTAACGAAGAGTTATTTTGACTGTAATCTGTAAAATTCATTTTTACAGTATCATTTCAATTCGTCTTCTGTTCAATAGCTTAGACTAAACGAGACATAATACGGCTGTTTATTGCCTATTGACATTCATTTGTTTCTTCTCTCTTAAGGATTTCACAGTTTATTTAATGTGGTTCCCCTTTCTATTGAAGGAAATTCCTAGTGTTGAAATGCCAAACACTGGCCCTTTTTTGTCCTTAATTTAACCGATTAAGGTTCGTAATAAGAGTCCTCTCGCATTCAATTGCAATTTATAGTTTTTTCCCTGGTCGCAGATAGCGGACGATTCTTCCAATTAATGTCCTTAGGCGTTTTAGGTTGTTATTAGAATTATCATTAATGTCAAAATTCAAAAGTTTAATGCGAGGTATAGACTTATATTACTATTACATCGCACCCTCAAACCTTCATCAATAAGATTGGCTCATTAGGTGGAATTTGGAAATACGATGTTGATCAATATTTCGATGATAGGTTATTATGAAAATGTGGGTATAAAAGAATGGATTGTCCTTCAAAGTTGTAAAAAACGAAAATGTAAATGGTATTCGGTCTATGATTTTTTTTTTAAATTACCAATATATTTTCTAATACGTTATGAAAGTTCTGATGATACTTACAAGGTTTAGAGGGATCATTTTTGTTGATACCTGTACTTGATGTGCTCATCATAGATAATATTGAGAAAACCTTTCAACTGCAAAGTTCTAATGATTACGGTTGAACAAATGTTATATGTTTTATCACATACGAATTCCAAAAGGTTAAAGTTTTGAGTTCTCATCTCCACGTGTTGTCAAACTAATTAATGTTTATGTTTTTTATTTTTCAATCTTTAAAAGTTGTATAGTTGGAAGATAGAATCTTCAACTTGAGGAAGACAATTGATGCTTTTTTATTAATGAGTGATGCTTAAATTGAAATTTAAATCTAATGTTTTTGATATCTACTTCAGATCATTTTATGGACTTTTTGTTTTTTTTTTTTTCCTAGATTTGCTTATCTATTTACCTTCAGTTTCTATAAATGTGTCTTAGTGGTGACCCAGCAGAGGCATAACCATATGAATAAAAATTAATTTGATACCTACACACAGCAAAAGCATGGAAAATCTATGTTCTAAAATTATGGATGCCTGATTCATTGGTGCTGGGTCATCGGTTTTGAAAGTACAACAAATATTGTTGTTCCTATGAGTAAAATCATTCAAATCAAGGTTTTTGTTTTTTGTTGTAAAGTTTATGTACCGGTAAACCTTAGTTTGTATGTGAAATGAGAAAATTGGTCTTGGACGATTGATTATGAAGTCATTATGCACACCAATCTTGTATTATGAGTTGCATTCAACAAGATTGCTCGTTGTTGTTTGAGAAGATTATTGAGTCATCTTGGGAAAAAGTATTGGATTTTGCGCTTGAATGTCAAGTAGGGAAATAATATTGTAGAGGTTTGTGGGCTGAACCTGTTATCTTCATTTTTAATATTAATTAAAGTTCCATGTAGACTCAAGTTGTTTTTTGATCAAGGTGAAAATGATGTCTTCAACCATATATTGTTTGGATTTGGACATGGGAAACCTCGTTAAGTATTGTACAGCTGTTATTTGTAGAAATATTAATGCCAAGTTCACTGTGACCAATGTATGTAGAAGAAATTAAAGGTGATGCTTTGTGTTAGTGTTTTAATGTACAGTTTCAATTTCATGAATCAGGTACAGTTACTCAATTAGTAGTCAGATTTTTTCTTGTAATCTTTTTGTTAGTCACAGTAGTAAGTTAGTTATTGTCCATGAGGCGGAAATAGATGTTGTGCTGGGGTTTGGTCCTAGTTCAAGATAGTTTTCCTAGAATGTTGTGTTCATCATTGTACACAAATTGCTTTATTAGGAAACAGGTTCGTTCACCATTGAATATTTCTTTCATTTTTACCATTGAAGTTCTAAATTTCTGTGTATCTGTGCCATTTTTGTGAAAAATTGATTTTGTCAGCTGGTATAGTTTAGTTTTGGAATATAATTGAAGCCAACGTTTCATAAAATTTCTTATTTGCAAATGTTGACTCAATTTTGTGAGGCATGATGTTTTTATTCTGTCCATTTTATTTTGGTGTACATATGTGTTTATATTTCCTGTAATATCGTTTAATTCATCATTCAAGAAGTTGATGGTATGTAGTTGATTATATCTTTCCTTTGATCAACACAAAACTCTTGGTGCTCAGTTCAATGCCAAGCATTTGTTGATTCCTATTGTGGTTTGCATCTTTGAAATTTTAGTATTACTGCTGATTACTTTAGGCATCTAGCTGATGTGCAAGTAAACTATTAGGGGATTTACCTCGTCTATCAATTTGATAAATTGACTTTATTCTAATAAGAGTATGATTGTGTCGTTATCCAGACTCCATGTGGCCACAATTTCTGCTTAAAATGTTTCCAGAAGTGGATTGGACAAGGAAAAAACACTTGTGCAAAGTGCCGCTGTGTGATTCCCTCCAAAATGGCTAGCCAGCCCCGTATTAATTCCACCCTTGTAGTGGCCATTCGTATGGCTAAAATGTCAAAATCCATTGGTTCTGGAGGACCTCAGAAGGTTTATCATTTTGTCCACAATCAAAATAGACCAGATAAAGCCTATACCACTGACCGGGCACAGAAAAAAGGGAAAGCTAATGCTGCAAGTGGAAAAATATTTGTTACAATTCCATCCGATCATTTTGGTCCAATTCCTGCTGAAAATGACCCTGAGAGAAACCAGGGTGTTCTGGTTGGTGAATGCTGGGAGGATAGGCTAGAATGTCGGCAATGGGGTGCTCACTTTCCACATGTTGCAGGTATAGCAGGCCAGTCAAATAATGGTGCTCAATCAGTGGTTCTTTCTGGTGGCTACCAGGATGACGAAGATCATGGGGAGTGGTTCCTCTACACTGGAAGGTTATTTTTTTTTTCTTTCTGTACTCTATGCAGTCTGTTCTTTCCCTGTCCTTGTTGGTTATGCAATATGTCCTTGTCTTTTTCCTGTTCTGCAAACTTTAAACGAGCCAGGTTTTCAGATGGCCTGCTCTTGTGTTAAATAAACTTATGATAAGATATTTTAGTTCATTTTTCATTGGTTAGTACCATTCCCCATCTTGTCTCTTCTACTTTCCTGTTTGTTTTTAGCTTCTTAAGATTTTGACCTGTTTGGTACAGAGTAAATTTGGTGGTATAATATTAACTTATTGCAATATTTTTTTTATTCTCTTTTACATTTTTCAGTCACTATGCATGATAAACCATTGTGTTTGATGATTTTTCCTGGATTTTAATTGCAGTGGGGGTCGAGACCTTAGTGGGAACAAACGTACGAGCAAGGATCAGTCTTTTGACCAGAAGTTTGAAAAATCAAACAAAGCCCTACAAGTTAGTTGCTTGAAAGGTTATCCTGTTAGGGTTGTTAGGTAGGTGCAGATTAATGCCTGATATTCTGTGATTTTGGGGTTTCTATTTCTGTGTTGTTGTAGGCCCATTTATTTGGTTTCAACCTTTGGTTATACAGAATATTTCTTTTTGAGACAGTTACAGAACGTGGTTTACAGTGCCTTGATGTCTTGATAATTCATTTTCTAGTTAAAATTTATGCTGTCATTCAGTTCAGCATTTTAATTTGGAGAGTTCATGACTCAGTATTCCAGAACGGTCGAAGAATTATTTGATTTTTGACTTATGTGGAACTGGAAAAGCACATGGTTTAAATTAGTCTCATTGATTTTATGTCCTGTGTAACGTGAGTAAACTTCTGATTTTAAAACTAAAGAAATGCCGTTAAAGTCGTTAAACTACAACTGTTTTGGTTGAGCATGTTCAACATGTTTTTCCATGCTGGGTAGAGGTATGGGAAATCCAGAAAACTCACTCATTCATCTGTCGCTTGTCAAAATTGCGCCTGCTTTTTCATGCCAGACTTGAAGTTTCCTTGTGCCTTTTTCAGTCGTGATCGCATAGCAATTGGTGAAATAGCTTAGGAAAATTAGTGTTACTCTTGGCTTCTTGATGTTGTATGTTGGAGTAGATCTTTAATCCAAGTAAGGGTGTGAAGTTTATTAATGCGGTATGTTAATCTTCTTGATAACAGATCTCACAAAGAGAAGCGTTCCTCGTATGCTCCAGAGAAGGGTTTGCGGTATGATGGAATTTATAGGATTGAGAAGTGTTGGCGTAAAGTGGGAATTCAGGTATTCAAGCTATTCCTCCTTTCCTAAACTACCATCTGCTGAACAAATTATTGATTAATATGTGTATCCTGAAATTAGGGCTTTAAGGTATGTCGGTATTTATTTGTTAGATGCGACAATGATCCTGCTCCATGGACAAGGTTTGTGTTTCCCAATAATGTAATTGCTTTTGTGTTATCAAATGCTAGTTGCAGTCTCTGGCTTTGCCTCCAAAGCTTTTTCCTTTCATTAAACAAGTTATTTAACTACAGCGATGAACATGGAGATCGACCTAGACCCTTACCTGCAATTCCAGAGTTAAAAAAGGCCACAGATATTACGGAGAGGAAGGAAGGTCCATCATGGGACTTTGATGTGAGTATACCCTAGTTATATTTCACTTGTGAGATCTCCTTCTCCTCCAATATTTCACTTTTATTAGATTATTAATTAAATCGAATCAGTTCCTTTGTCTGAATATTAGTTTTTCTAACGATACAAGTTCTTTGAAAAGATAGCGAGCTTTCAAGTTTTGGTTGTTAGTAACCAAGGGCAGGTAGGCACTAGATAGCCTCTTTGTTGTAATACTATCTAATTTGAAGAACCCTTCAAAATCTTTATAATGTTTGGTTATGTAATCAATGTTTTAAAAGGCTAAAGGTTGAGGCTTTTCCTCTTGAAGAGGCGAAGCACAAGCCTCGAGGCGGTGTGAGGGGTAAGCTTCAATTTTAATTAAAAACATTACGTAAATCCTAAATTTGTGTGAACAAGTAATGCTAAACATAATACTTATATAAAAGTATAGAAAAAGTCACCCCTTGAATTGCTACTTGAACAAAAATTCTACAAATATATCTTTAACATCACTACTATTATCACTTATATGATTGACTTATTCTATACGTTTTTATTTTCAATGTTTTTAAAGCCTAAGTGGTAAAAGTAGTAATATTTTAAATTGTAACAACTCAAACCTTTATATTTTTAAAGAATAGGGAAATTAATACTATTCTTCTAATCATTATATGAGGCGCCTCAGCGCCTTTTTATAAAGGCATAAGCCTCTCCTGTGAGAGGCGTAAGTCTCCTATGGTCCAAAGAAGGCGTAAGTCTCGAGACTAAAAGTTATAGCTTCGCCTCGAGGCGAGCCTCAAGGCATAAGTCTCAATAACCTTTTAAAACATTGTATATAATCAAAAGTAATTCTTAAAATGTTTTCAGACTTGCGCTAAATGAATTAGCTGTTGCAAATTTGTGTGCATATCCTGTTTTAAAGACTTTTCATATACTCATATTTTCAGTCTGTTCGATAATTTCTTTTTCATTCTTTTTCTGTTTCTGAAAAATAAGTTTTGAAATACTAGTAAAAAAAGCTAAGATAGTTTCTAAAAGTTGTTTATGTTCTAGCATTTAGCTTACATTTTGGTTATATTTTTAAATTTTTAAATATCTAGAGCAATTACGAAATATTGTTTTACTAAAGGAACTTACCTGTATAGAAACAAAGGGAAAAGAAAAACAAAAAATCTAATAAACGTCACGTACATTGTTAGAGTTTTATAAGAATCTAAGAATTTATGAAATCTTGTGTATGTAATCTATTTCAAGGATTTTTCCCCTTAAAGAAAATTGATTTTGACCTTAAGATTAAAACAATGATTTTAATAAGTGAAAGGCAGTGCCAACAAAATGAAGTTGTTCTTTTCTGAACGAGTTTTAATTTTTGACTAGTCTAAAATATTCATAAATTTCTGTGAACCAGTTTTAGTATAAACTAAAGTTGTTTCTACTAGCATTCATCTACTTTCAAAGATGGAAATTGTTTAGAAAAATTTTCTTGCCACCAAAACATAATTTTTATTTTTAAGAGTAAAATAAAATTGTATTTAAAAGTAGTCCTAATTGGCCTTTCAATTTTTCTGACACTTCCAATTTTGTGAAATGCAGTTTTACTTGTATATTATTTCCTTGCGTTTGGGCAAATACTGTATTTAAGTGATATAATCTAAAGCTTGTGAAATTGAAAGGCGAAATTATGTAGATTTGCCTGGTTTAGATTTGGTTGGTTCTAGTGTGGCTAGTCTCTTGCGGAATAAAATTTAAGATTGTAGATACCGTGGTGTTTGGTTCACTCAGTTCTATAACCATTTATCTAAGCTAATAAATGATAAATGAAGTGTCTTGTGGTAAATTTATGAACCCTCTGCAAGTTCTTTTTCAGGAGAAAGATAGTCGGTGGAAGTGGAGCAAAGCCCCACCAACGAGCAAGAAACCAGTTGAAACCGAAGATTCTGCTTCTGGGAAAAGGTCAAGGAGAAGAATTAGGCAATCGCATAATATGAGTGTCCGAGAAAGGTTACTGAAAGGTTGTCTAATTATATTGAGTAAAGTCGCTAAAAAATGAAGGCGGTTTGGTGTTGAACATGACGAGGCATTACTAACGACACGTTTTTTTTTTTAACATATTCCATTTGCTGCATGTAACTGTGTATGCAGAGTTTAGCTGCCTTATTTGTCGGGAGGTAATGAGTCTGCCAATCACTACGCCTTGTGCCCATAACTTTTGCAAGACATGCTTGGAAGGTGCTTTTGTGGGCAAGACATTCTTGAGAGAGAGAAGCAGTGGTGGACGGGCCCTTAGATCCCAAAAGAATGTCATGAGTTGTCCTTGTTGCCCGACAGATATATCTGATTTTCTACAGAATCTCCAGGTGTGTGTCGCGAAAGATTCTTTTTGTTTTAACATGAAGAAAATTGTTTCTCTCTCACTTGGCTAGTTGCTACTATCAATAGGTAAATAGAGAGTTGTTGGATGTGATCGAGTCATTGAAAAGTAAGATTGAAGAAGGGGATGCATCATCTGAGAAGCTCTGTGAAGAAGAGATAGAAGGGGAAGAGGATGAGGAAGAGGAGGAGGGAAAAGATGGAAGCAGCAGTCCTGTGGAAACAAGAAAGCGAGCAAAGGCGGTGGTTAATGGTGGGGAGGAAAATGCTGTGGCCGGGAATGGAGATGATTCACCCTCAAGTCCCCTCCACGTGGATGATTTGGAATGAAGTAGAGACAATTCAGGGGTATTTTTTTTTTGATGGGTTCGGGTACTAGTATAATTTTGGTAGATATGCGTCTCCTGTCATTTGATTTGAAGACAATATCAAATGGCAATGTCGTTTACTTCTTGGAGACGCTAGACAGACACAGTGTTATTGTATTTTCTTTTCGAGAACTTGTGTAAATGGGTCAGTTGTACTTGTATTTTCTTTACACAGTGATGGCATCATAGGTCGAGTAGAATGTACTTGTTTGTAATACACAGTGATGGCATGATTCATGGGTCCTTCCATTCCCATTCCAACTGATTGATTTCTTCTATAAATTCTTTGGTTTAGGTTTGAACATGCCGAGAAAGATGCGGAAAGTCATTGTCTTTGGGCCGTAAACGTTGACAACCAAGTCCAGGAGCCGAAAGACCAAAGTTCTAAAACACCGCTTGTCGGCCGCTCACTTATTTTATTTATTGTTTGAAGGAAAGCCATTTTATTG

mRNA sequence

ATGGCGCAGGTTTCTCAGCTGCCTTGCGACGGCGATGGCATTTGCATGCTCTGCCAGGCCAAGCCTTCCGACTTGGAGACGATCACTTGCAAAACTTGCGTCACTCCGTGGCACGTCGGTTGTCTCTCAAATCCCCCTGAAACTCTGGCATCTACCCTTCAATGGGACTGTCCCGACTGTTCCCCCCCTCGCGAAGATGCTCCCCTTCCTCCACCCGGCAACTCTCCACTCCCCACTGCGCCTTCCAGTGACCTGGTTGCTGCGATTCGAGCCATTGAGGCCGATGCCTCCCTTACTGACCGCGAGAAGGCAAAGAAGCGCCAGGAGCTTCTTAGCGGAAAGTCTCAGTCCGAGAAGGATGATCATGATGCCAACAAGGAAAAGAGAAAAAGAGGGGACGATGTTTTGGATTTGTTCGACGAGAGATTGAACTGCTCCTTCTGCATGCAGTTACCAGAAAGGCCAGTCACTACTCCATGTGGCCACAATTTCTGCTTAAAATGTTTCCAGAAGTGGATTGGACAAGGAAAAAACACTTGTGCAAAGTGCCGCTGTGTGATTCCCTCCAAAATGGCTAGCCAGCCCCGTATTAATTCCACCCTTGTAGTGGCCATTCGTATGGCTAAAATGTCAAAATCCATTGGTTCTGGAGGACCTCAGAAGGTTTATCATTTTGTCCACAATCAAAATAGACCAGATAAAGCCTATACCACTGACCGGGCACAGAAAAAAGGGAAAGCTAATGCTGCAAGTGGAAAAATATTTGTTACAATTCCATCCGATCATTTTGGTCCAATTCCTGCTGAAAATGACCCTGAGAGAAACCAGGGTGTTCTGGTTGGTGAATGCTGGGAGGATAGGCTAGAATGTCGGCAATGGGGTGCTCACTTTCCACATGTTGCAGGTATAGCAGGCCAGTCAAATAATGGTGCTCAATCAGTGGTTCTTTCTGGTGGCTACCAGGATGACGAAGATCATGGGGAGTGGTTCCTCTACACTGGAAGTGGGGGTCGAGACCTTAGTGGGAACAAACGTACGAGCAAGGATCAGTCTTTTGACCAGAAGTTTGAAAAATCAAACAAAGCCCTACAAGTTAGTTGCTTGAAAGGTTATCCTGTTAGGGTTGTTAGATCTCACAAAGAGAAGCGTTCCTCGTATGCTCCAGAGAAGGGTTTGCGGTATGATGGAATTTATAGGATTGAGAAGTGTTGGCGTAAAGTGGGAATTCAGGGCTTTAAGGTATGTCGGTATTTATTTGTTAGATGCGACAATGATCCTGCTCCATGGACAAGGTTTGTGTTTCCCAATAATGTAATTGCTTTTGTGTTATCAAATGCTAGTTGCAGTCTCTGGCTTTGCCTCCAAAGCTTTTTCCTTTCATTAAACAAGTTATTTAACTACAGCGATGAACATGGAGATCGACCTAGACCCTTACCTGCAATTCCAGAGTTAAAAAAGGCCACAGATATTACGGAGAGGAAGGAAGGTCCATCATGGGACTTTGATGAGAAAGATAGTCGGTGGAAGTGGAGCAAAGCCCCACCAACGAGCAAGAAACCAGTTGAAACCGAAGATTCTGCTTCTGGGAAAAGGTCAAGGAGAAGAATTAGGCAATCGCATAATATGAGTGTCCGAGAAAGGTTACTGAAAGAGTTTAGCTGCCTTATTTGTCGGGAGGTAATGAGTCTGCCAATCACTACGCCTTGTGCCCATAACTTTTGCAAGACATGCTTGGAAGGTGCTTTTGTGGGCAAGACATTCTTGAGAGAGAGAAGCAGTGGTGGACGGGCCCTTAGATCCCAAAAGAATGTCATGAGTTGTCCTTGTTGCCCGACAGATATATCTGATTTTCTACAGAATCTCCAGGTAAATAGAGAGTTGTTGGATGTGATCGAGTCATTGAAAAGTAAGATTGAAGAAGGGGATGCATCATCTGAGAAGCTCTGTGAAGAAGAGATAGAAGGGGAAGAGGATGAGGAAGAGGAGGAGGGAAAAGATGGAAGCAGCAGTCCTGTGGAAACAAGAAAGCGAGCAAAGGCGGTGGTTAATGGTGGGGAGGAAAATGCTGTGGCCGGGAATGGAGATGATTCACCCTCAAGTCCCCTCCACGTGGATGATTTGGAATGA

Coding sequence (CDS)

ATGGCGCAGGTTTCTCAGCTGCCTTGCGACGGCGATGGCATTTGCATGCTCTGCCAGGCCAAGCCTTCCGACTTGGAGACGATCACTTGCAAAACTTGCGTCACTCCGTGGCACGTCGGTTGTCTCTCAAATCCCCCTGAAACTCTGGCATCTACCCTTCAATGGGACTGTCCCGACTGTTCCCCCCCTCGCGAAGATGCTCCCCTTCCTCCACCCGGCAACTCTCCACTCCCCACTGCGCCTTCCAGTGACCTGGTTGCTGCGATTCGAGCCATTGAGGCCGATGCCTCCCTTACTGACCGCGAGAAGGCAAAGAAGCGCCAGGAGCTTCTTAGCGGAAAGTCTCAGTCCGAGAAGGATGATCATGATGCCAACAAGGAAAAGAGAAAAAGAGGGGACGATGTTTTGGATTTGTTCGACGAGAGATTGAACTGCTCCTTCTGCATGCAGTTACCAGAAAGGCCAGTCACTACTCCATGTGGCCACAATTTCTGCTTAAAATGTTTCCAGAAGTGGATTGGACAAGGAAAAAACACTTGTGCAAAGTGCCGCTGTGTGATTCCCTCCAAAATGGCTAGCCAGCCCCGTATTAATTCCACCCTTGTAGTGGCCATTCGTATGGCTAAAATGTCAAAATCCATTGGTTCTGGAGGACCTCAGAAGGTTTATCATTTTGTCCACAATCAAAATAGACCAGATAAAGCCTATACCACTGACCGGGCACAGAAAAAAGGGAAAGCTAATGCTGCAAGTGGAAAAATATTTGTTACAATTCCATCCGATCATTTTGGTCCAATTCCTGCTGAAAATGACCCTGAGAGAAACCAGGGTGTTCTGGTTGGTGAATGCTGGGAGGATAGGCTAGAATGTCGGCAATGGGGTGCTCACTTTCCACATGTTGCAGGTATAGCAGGCCAGTCAAATAATGGTGCTCAATCAGTGGTTCTTTCTGGTGGCTACCAGGATGACGAAGATCATGGGGAGTGGTTCCTCTACACTGGAAGTGGGGGTCGAGACCTTAGTGGGAACAAACGTACGAGCAAGGATCAGTCTTTTGACCAGAAGTTTGAAAAATCAAACAAAGCCCTACAAGTTAGTTGCTTGAAAGGTTATCCTGTTAGGGTTGTTAGATCTCACAAAGAGAAGCGTTCCTCGTATGCTCCAGAGAAGGGTTTGCGGTATGATGGAATTTATAGGATTGAGAAGTGTTGGCGTAAAGTGGGAATTCAGGGCTTTAAGGTATGTCGGTATTTATTTGTTAGATGCGACAATGATCCTGCTCCATGGACAAGGTTTGTGTTTCCCAATAATGTAATTGCTTTTGTGTTATCAAATGCTAGTTGCAGTCTCTGGCTTTGCCTCCAAAGCTTTTTCCTTTCATTAAACAAGTTATTTAACTACAGCGATGAACATGGAGATCGACCTAGACCCTTACCTGCAATTCCAGAGTTAAAAAAGGCCACAGATATTACGGAGAGGAAGGAAGGTCCATCATGGGACTTTGATGAGAAAGATAGTCGGTGGAAGTGGAGCAAAGCCCCACCAACGAGCAAGAAACCAGTTGAAACCGAAGATTCTGCTTCTGGGAAAAGGTCAAGGAGAAGAATTAGGCAATCGCATAATATGAGTGTCCGAGAAAGGTTACTGAAAGAGTTTAGCTGCCTTATTTGTCGGGAGGTAATGAGTCTGCCAATCACTACGCCTTGTGCCCATAACTTTTGCAAGACATGCTTGGAAGGTGCTTTTGTGGGCAAGACATTCTTGAGAGAGAGAAGCAGTGGTGGACGGGCCCTTAGATCCCAAAAGAATGTCATGAGTTGTCCTTGTTGCCCGACAGATATATCTGATTTTCTACAGAATCTCCAGGTAAATAGAGAGTTGTTGGATGTGATCGAGTCATTGAAAAGTAAGATTGAAGAAGGGGATGCATCATCTGAGAAGCTCTGTGAAGAAGAGATAGAAGGGGAAGAGGATGAGGAAGAGGAGGAGGGAAAAGATGGAAGCAGCAGTCCTGTGGAAACAAGAAAGCGAGCAAAGGCGGTGGTTAATGGTGGGGAGGAAAATGCTGTGGCCGGGAATGGAGATGATTCACCCTCAAGTCCCCTCCACGTGGATGATTTGGAATGA

Protein sequence

MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDCSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKDDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEDEEEEEGKDGSSSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE
Homology
BLAST of Spg007450 vs. NCBI nr
Match: XP_022134816.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 613/709 (86.46%), Postives = 630/709 (88.86%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICMLC+AKPSDLETITCKTCVTPWHV CLSNPPETLASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMLCKAKPSDLETITCKTCVTPWHVACLSNPPETLASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S P  D PLPP      PTAPSS LVAAIRAIEADA LTDR+KAKKRQELL+G S S+KD
Sbjct: 61  SSPPGDVPLPP------PTAPSSHLVAAIRAIEADAFLTDRQKAKKRQELLTGNSHSDKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           D DA   K   GDDVL+LFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DRDATNRKSLIGDDVLELFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSI SGGPQKVYHFVHNQNRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIVSGGPQKVYHFVHNQNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQS+NGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSSNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPR LPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRALPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERKEGPSWDFDEKDSRWKWSK PPTSKKPVETEDSASGKRSRR++RQS 
Sbjct: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKPPPTSKKPVETEDSASGKRSRRKVRQSQ 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NMSVRE+LLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAFVGKTFLRERSSGGRALRS
Sbjct: 541 NMSVREKLLKEFSCLICREVMTLPITTPCAHNFCKSCLEGAFVGKTFLRERSSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEDEE 660
           QKNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGD +SEKLCEE  +G     
Sbjct: 601 QKNVMTCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGD-TSEKLCEEGTDG----- 660

Query: 661 EEEGKDGSSS-PVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
           EEEGK+ SS   VETRKRAK V +GGEEN V G+GDDSPSSPLHVDDLE
Sbjct: 661 EEEGKECSSPVGVETRKRAKVVADGGEENGVGGDGDDSPSSPLHVDDLE 660

BLAST of Spg007450 vs. NCBI nr
Match: XP_023531327.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 615/716 (85.89%), Postives = 633/716 (88.41%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPP EDA LPPP + PLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDAN+   KRGDDVLDLFDERL CSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SGG QKVYH VHN NRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISGGSQKVYHVVHNLNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPRPLPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRPLPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERK+ PSWDFDEKDSRWKWSK PPTSKK VETEDS SGK+SRRR+RQSH
Sbjct: 481 IPELKKATDITERKKDPSWDFDEKDSRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSH 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM+VRERLLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS
Sbjct: 541 NMNVRERLLKEFSCLICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE---E 660
           +KNVM+CPCCPTDISDFLQNLQVNRELLDVIE+LKSKIEE DA SEK CE+EIEGE   E
Sbjct: 601 RKNVMACPCCPTDISDFLQNLQVNRELLDVIETLKSKIEEADA-SEKPCEDEIEGEGEGE 660

Query: 661 DEEEEEGKDG-----SSSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
            E+EEEGKDG     SS PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 661 QEQEEEGKDGSNGNESSRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Spg007450 vs. NCBI nr
Match: XP_022965844.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita maxima])

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 614/716 (85.75%), Postives = 631/716 (88.13%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPP EDA LPPP + PLP  PS+DLV AIRAIEADASLTDREKA KRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVTAIRAIEADASLTDREKANKRQELLSGKSQSEKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDAN+   KRGDDVLDLFDERL CSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SGG QKVYH VHN NRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISGGSQKVYHVVHNLNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPRPLPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRPLPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERK+ PSWDFDEKDSRWKWSK PPTSKK VETEDS SGK+SRRR+RQSH
Sbjct: 481 IPELKKATDITERKKDPSWDFDEKDSRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSH 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM+VRERLLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS
Sbjct: 541 NMNVRERLLKEFSCLICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE---E 660
           +KNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE   E
Sbjct: 601 RKNVMACPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGE 660

Query: 661 DEEEEEGKDG-----SSSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
            E+EEEGKDG     SS PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 661 GEQEEEGKDGSNGNESSRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Spg007450 vs. NCBI nr
Match: XP_022933744.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita moschata])

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 614/720 (85.28%), Postives = 632/720 (87.78%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPP EDA LPPP + PLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDAN+   KRGDDVLDLFDERL CSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SGG QKVYH VHN NRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISGGSQKVYHVVHNLNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPRPLPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRPLPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERK+ PSWDFDEKD+RWKWSK PPTSKK VETEDS SGK+SRRR+RQSH
Sbjct: 481 IPELKKATDITERKKDPSWDFDEKDNRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSH 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM+VRERLLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS
Sbjct: 541 NMNVRERLLKEFSCLICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE---- 660
           +KNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE    
Sbjct: 601 RKNVMACPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGE 660

Query: 661 ---EDEEEEEGKDG-----SSSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
              E E+EEEGKDG     SS PVETRKRAK VVNGGEEN        SPSSP+HVDDLE
Sbjct: 661 EEQEQEQEEEGKDGSNGNESSRPVETRKRAKVVVNGGEENY-------SPSSPVHVDDLE 672

BLAST of Spg007450 vs. NCBI nr
Match: KAG6587385.1 (E3 ubiquitin-protein ligase ORTHRUS 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021368.1 E3 ubiquitin-protein ligase ORTHRUS 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 613/716 (85.61%), Postives = 631/716 (88.13%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPP EDA LPPP + PLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDAN+   KRGDDVLDLFDERL CSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SGG QKVYH VHN NRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISGGSQKVYHVVHNLNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RC+NDPAPWT                                     SDEHGDRPRPLPA
Sbjct: 421 RCENDPAPWT-------------------------------------SDEHGDRPRPLPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERK+ PSWDFDE DSRWKWSK PPTSKK VE EDS SGK+SRRR+RQSH
Sbjct: 481 IPELKKATDITERKKDPSWDFDENDSRWKWSKPPPTSKKSVEPEDSTSGKKSRRRMRQSH 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM+VRERLLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS
Sbjct: 541 NMNVRERLLKEFSCLICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEG---EE 660
           +KNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEG   EE
Sbjct: 601 RKNVMACPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEEE 660

Query: 661 DEEEEEGKDGS-----SSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
            E+EEEGKDGS     S PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 661 QEQEEEGKDGSNGNEISRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Spg007450 vs. ExPASy Swiss-Prot
Match: Q8VYZ0 (E3 ubiquitin-protein ligase ORTHRUS 2 OS=Arabidopsis thaliana OX=3702 GN=ORTH2 PE=1 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 2.6e-249
Identity = 436/667 (65.37%), Postives = 503/667 (75.41%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA+  QLPCDGDG+CM C++ P   E++TC TCVTPWHV CLS+PP+TLASTLQW CPDC
Sbjct: 1   MARDIQLPCDGDGVCMRCKSNPPPEESLTCGTCVTPWHVSCLSSPPKTLASTLQWHCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S   E  PLP  G +    +  SDLVAAIRAIEAD SL+  EKAK RQ LLSGK   E D
Sbjct: 61  S--GEIDPLPVSGGATGFESAGSDLVAAIRAIEADESLSTEEKAKMRQRLLSGKGVEEDD 120

Query: 121 DHDANKEKRKRGD---DVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGK 180
           + +  K+K K  +   DVL    + L CSFCMQLPERPVT PCGHN CLKCF+KW+GQGK
Sbjct: 121 EEEKRKKKGKGKNPNLDVLSALGDNLMCSFCMQLPERPVTKPCGHNACLKCFEKWMGQGK 180

Query: 181 NTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYT 240
            TC KCR +IP KMA  PRINS+LV AIR+AK+SKS  +    KV+HF+ NQ+RPDKA+T
Sbjct: 181 RTCGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKS-AAATTSKVFHFISNQDRPDKAFT 240

Query: 241 TDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHF 300
           T+RA+K GKANAASGKI+VTIP DHFGPIPAENDP RNQG+LVGE WEDRLECRQWGAHF
Sbjct: 241 TERAKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGLLVGESWEDRLECRQWGAHF 300

Query: 301 PHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFE 360
           PHVAGIAGQS  GAQSV LSGGY+DDEDHGEWFLYTGSGGRDLSGNKRT+K+QSFDQKFE
Sbjct: 301 PHVAGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGRDLSGNKRTNKEQSFDQKFE 360

Query: 361 KSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQG-FKVCR 420
           KSN AL++SC  GYPVRVVRSHKEKRS+YAPE+G+RYDG+YRIEKCWRKVG+QG FKVCR
Sbjct: 361 KSNAALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVYRIEKCWRKVGVQGSFKVCR 420

Query: 421 YLFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPR 480
           YLFVRCDN+PAPWT                                     SDE+GDRPR
Sbjct: 421 YLFVRCDNEPAPWT-------------------------------------SDENGDRPR 480

Query: 481 PLPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRI 540
           P+P IPEL  ATD+ ERKE PSWDFDE +  WKW K PP SKK V        K  R+ I
Sbjct: 481 PIPNIPELNMATDLFERKETPSWDFDEGEGCWKWMKPPPASKKSVNVLAPEERKNLRKAI 540

Query: 541 RQSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGR 600
           + +H+ ++R RLLKEF C IC++V++LP+TTPCAHNFCK CLE  F GKT +RERS+GGR
Sbjct: 541 KAAHSNTMRARLLKEFKCQICQQVLTLPVTTPCAHNFCKACLEAKFAGKTLVRERSTGGR 600

Query: 601 ALRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE 660
            LRS+KNV++CPCCPTDISDFLQN QVNRE+ +VIE LK++ EE  A  E   E E  G 
Sbjct: 601 TLRSRKNVLNCPCCPTDISDFLQNPQVNREVAEVIEKLKTQ-EEDTAELEDEDEGECSGT 626

Query: 661 EDEEEEE 664
             EE+ E
Sbjct: 661 TPEEDSE 626

BLAST of Spg007450 vs. ExPASy Swiss-Prot
Match: Q9FKA7 (E3 ubiquitin-protein ligase ORTHRUS 1 OS=Arabidopsis thaliana OX=3702 GN=ORTH1 PE=1 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 5.7e-220
Identity = 399/672 (59.38%), Postives = 476/672 (70.83%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST +W+CPD
Sbjct: 1   MAIETQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVPCLL--PESLASSTGEWECPD 60

Query: 61  CSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  GN+  P +  S LVAAIRAI+AD +LT+ EKAKKRQ+L+SG      
Sbjct: 61  CSGVVVPSAAPGTGNA-RPESSGSVLVAAIRAIQADETLTEAEKAKKRQKLMSGGG---- 120

Query: 121 DDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKW-IGQGKN 180
           DD    +EK+K     L++F     CS C+QLPERP+TTPCGHNFCLKCF+KW +GQGK 
Sbjct: 121 DDGVDEEEKKK-----LEIF-----CSICIQLPERPITTPCGHNFCLKCFEKWAVGQGKL 180

Query: 181 TCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTT 240
           TC  CR  IP  +A  PRIN  LV AIR+A ++K        KV+H + NQ+RP+KA+TT
Sbjct: 181 TCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSVEATAAKVHHIIRNQDRPEKAFTT 240

Query: 241 DRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFP 300
           +RA K GKANAASGK FVTIP DHFGPIPAEND  R QGVLVGE WEDR ECRQWGAHFP
Sbjct: 241 ERAVKTGKANAASGKFFVTIPRDHFGPIPAENDVTRKQGVLVGESWEDRQECRQWGAHFP 300

Query: 301 HVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEK 360
           H+AGIAGQS  GAQSV LSGGY DDEDHGEWFLYTGSGGRDLSGNKR +K QS DQ F+ 
Sbjct: 301 HIAGIAGQSAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRINKKQSSDQAFKN 360

Query: 361 SNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQG-FKVCRY 420
            N++L++SC  GYPVRVVRS KEKRS+YAP +G+RYDG+YRIEKCW  VG+QG FKVCRY
Sbjct: 361 MNESLRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGSFKVCRY 420

Query: 421 LFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRP 480
           LFVRCDN+PAPWT                                     SDEHGDRPRP
Sbjct: 421 LFVRCDNEPAPWT-------------------------------------SDEHGDRPRP 480

Query: 481 LPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIR 540
           LP +PEL+ A D+  RKE PSWDFDE + RWKW K+PP S+  ++ E+    KR++    
Sbjct: 481 LPNVPELETAADLFVRKESPSWDFDEAEGRWKWMKSPPVSRMALDPEERKKNKRAKN--- 540

Query: 541 QSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRA 600
                +++ RLLKEFSC ICREV+SLP+TTPCAHNFCK CLE  F G T LRERS+GGR 
Sbjct: 541 -----TMKARLLKEFSCQICREVLSLPVTTPCAHNFCKACLEAKFAGITQLRERSNGGRK 600

Query: 601 LRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEE 660
           LR++KN+M+CPCC TD+S+FLQN QVNRE++++IE+ K   EE DAS   + EEE E  E
Sbjct: 601 LRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEADAS---ISEEEEEESE 607

Query: 661 DEEEEEGKDGSS 670
              ++   D +S
Sbjct: 661 PPTKKIKMDNNS 607

BLAST of Spg007450 vs. ExPASy Swiss-Prot
Match: Q680I0 (E3 ubiquitin-protein ligase ORTHRUS 5 OS=Arabidopsis thaliana OX=3702 GN=ORTH5 PE=2 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 8.3e-219
Identity = 395/663 (59.58%), Postives = 469/663 (70.74%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST  W+CPD
Sbjct: 1   MAIQTQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVSCLL--PESLASSTGDWECPD 60

Query: 61  CSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  G S  P +  S LV AIRAI+AD +LT+ EKAKKRQ L+SG      
Sbjct: 61  CSGVVVPSAAPGTGISG-PESSGSVLVTAIRAIQADVTLTEAEKAKKRQRLMSGGGDDGV 120

Query: 121 DDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKW-IGQGKN 180
           DD    +EK+K     L++F     CS C+QLPERPVTTPCGHNFCLKCF+KW +GQGK 
Sbjct: 121 DD----EEKKK-----LEIF-----CSICIQLPERPVTTPCGHNFCLKCFEKWAVGQGKL 180

Query: 181 TCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTT 240
           TC  CR  IP  +A  PRIN  LV AIR+A ++K  G     KV+H + NQ+RPDKA+TT
Sbjct: 181 TCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGEATAAKVHHIIRNQDRPDKAFTT 240

Query: 241 DRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFP 300
           +RA K GKANAASGK FVTIP DHFGPIPA ND  RNQGVLVGE WEDR ECRQWG HFP
Sbjct: 241 ERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQGVLVGESWEDRQECRQWGVHFP 300

Query: 301 HVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEK 360
           HVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSGGRDLSGNKR +K QS DQ F+ 
Sbjct: 301 HVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRVNKIQSSDQAFKN 360

Query: 361 SNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGF-KVCRY 420
            N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG+YRIEKCW  VG+QG  K+CRY
Sbjct: 361 MNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGLHKMCRY 420

Query: 421 LFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRP 480
           LFVRCDN+PAPWT                                     SDEHGDRPRP
Sbjct: 421 LFVRCDNEPAPWT-------------------------------------SDEHGDRPRP 480

Query: 481 LPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIR 540
           LP +PEL+ ATD+  RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++   
Sbjct: 481 LPDVPELENATDLFVRKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK--- 540

Query: 541 QSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRA 600
              N +++ RLLKEFSC ICR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R 
Sbjct: 541 --GNNAMKARLLKEFSCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRK 600

Query: 601 LRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEE 660
           LR++KN+M+CPCC TD+S+FLQN QVNRE++++IE+ K   EE + +      EE EGEE
Sbjct: 601 LRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEE-EGEE 603

BLAST of Spg007450 vs. ExPASy Swiss-Prot
Match: Q9C8E1 (Putative E3 ubiquitin-protein ligase ORTHRUS 4 OS=Arabidopsis thaliana OX=3702 GN=ORTH4 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 4.1e-218
Identity = 398/673 (59.14%), Postives = 474/673 (70.43%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST  W+CPD
Sbjct: 1   MAIQTQLPCDGDGVCMRCQVTPPSEETLTCGTCVTPWHVSCLL--PESLASSTGDWECPD 60

Query: 61  CSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  G S  P +  S LVAAIRAI+AD +LT+ EKAKKRQ L+SG      
Sbjct: 61  CSGVVVPSAAPGTGISG-PESSGSVLVAAIRAIQADVTLTEAEKAKKRQRLMSGGGDDGV 120

Query: 121 DDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKW-IGQGKN 180
           DD    +EK+K     L++F     CS C+QLPERPVTTPCGHNFCLKCF+KW +GQGK 
Sbjct: 121 DD----EEKKK-----LEIF-----CSICIQLPERPVTTPCGHNFCLKCFEKWAVGQGKL 180

Query: 181 TCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTT 240
           TC  CR  IP  +A  PRIN  LV AIR+A ++K  G     KV+H + NQ+RPDKA+TT
Sbjct: 181 TCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGEATAAKVHHIIRNQDRPDKAFTT 240

Query: 241 DRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFP 300
           +RA K GKANAASGK FVTIP DHFGPIPA ND  RNQGVLVGE WEDR ECRQWG HFP
Sbjct: 241 ERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQGVLVGESWEDRQECRQWGVHFP 300

Query: 301 HVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEK 360
           HVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSGGRDLSGNKR +K QS DQ F+ 
Sbjct: 301 HVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRVNKIQSSDQAFKN 360

Query: 361 SNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGF-KVCRY 420
            N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG+YRIEKCW  VG+QG  K+CRY
Sbjct: 361 MNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGLHKMCRY 420

Query: 421 LFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRP 480
           LFVRCDN+PAPWT                                     SDEHGDRPRP
Sbjct: 421 LFVRCDNEPAPWT-------------------------------------SDEHGDRPRP 480

Query: 481 LPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIR 540
           LP +PEL+ ATD+  RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++   
Sbjct: 481 LPDVPELENATDLFVRKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK--- 540

Query: 541 QSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRA 600
              N +++ RLLKEFSC ICR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R 
Sbjct: 541 --GNNAMKARLLKEFSCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRK 600

Query: 601 LRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGD-ASSEKLCEEEIEGE 660
           LR++KN+M+CPCC TD+S+FLQN QVNRE++++IE+ K   EE + A S  + EEE E  
Sbjct: 601 LRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEEEEEES 614

Query: 661 EDEEEEEGKDGSS 670
           E   ++   D +S
Sbjct: 661 EPPTKKIKMDNNS 614

BLAST of Spg007450 vs. ExPASy Swiss-Prot
Match: Q9FVS2 (E3 ubiquitin-protein ligase ORTHRUS 3 OS=Arabidopsis thaliana OX=3702 GN=ORTH3 PE=3 SV=2)

HSP 1 Score: 742.3 bits (1915), Expect = 5.2e-213
Identity = 392/703 (55.76%), Postives = 480/703 (68.28%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           M   +Q PCD +G+CM C++ P   E++TC TCVTPWHV CL +PPETL++TLQW CPDC
Sbjct: 1   MTPATQYPCDPEGVCMRCKSMPPPEESLTCGTCVTPWHVSCLLSPPETLSATLQWLCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S   E  PLP  G +    +  SDLVAAI +IEAD +L+  EKAKK+Q+LLSGK   ++D
Sbjct: 61  S--GETNPLPVSGVAAGYGSVGSDLVAAIHSIEADETLSAEEKAKKKQQLLSGKGVVDED 120

Query: 121 DHDANKE--KRKRGDDVLDLFDERLNCSFCMQLPERPV-----------------TTPCG 180
           D +  K+  K K+  DVL  F+    CSFCMQ  ++PV                 +TPCG
Sbjct: 121 DEEEKKKTSKGKKPIDVLSHFE----CSFCMQSLQKPVSVRVLFALALMLVWFLESTPCG 180

Query: 181 HNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQK 240
           HN CLKCF KW+GQG  +C  CR VIP  M + PRIN ++V AIR+A++S+        K
Sbjct: 181 HNACLKCFLKWMGQGHRSCGTCRSVIPESMVTNPRINLSIVSAIRLARVSEK-ADARTSK 240

Query: 241 VYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVG 300
           V H+V N++RPDKA+TT+RA+K G ANA+SGKIFVTIP DHFGPIPAENDP RNQG+LVG
Sbjct: 241 VVHYVDNEDRPDKAFTTERAKKTGNANASSGKIFVTIPRDHFGPIPAENDPVRNQGLLVG 300

Query: 301 ECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLS 360
           E W+ RL CRQWGAHFPHV+GIAGQ++ GAQSVVL+GGY DDEDHGEWFLYTGSGGR L 
Sbjct: 301 ESWKGRLACRQWGAHFPHVSGIAGQASYGAQSVVLAGGYDDDEDHGEWFLYTGSGGRILK 360

Query: 361 GNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKG-LRYDGIYRI 420
           GNKRT+  Q+FDQ F   N+AL++SC  GYPVRVVRS K+KRS YAP+ G LRYDG+YRI
Sbjct: 361 GNKRTNTVQAFDQVFLNFNEALRLSCKLGYPVRVVRSTKDKRSPYAPQGGLLRYDGVYRI 420

Query: 421 EKCWRKVGIQGFKVCRYLFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLS 480
           EKCWR VGIQ   +CR+LFVRCDN+PAPWT                              
Sbjct: 421 EKCWRIVGIQ---MCRFLFVRCDNEPAPWT------------------------------ 480

Query: 481 LNKLFNYSDEHGDRPRPLPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKP 540
                  SDEHGDRPRPLP +PEL  ATD+ ERKE PSWDFDE + RW+W K PP SKK 
Sbjct: 481 -------SDEHGDRPRPLPNVPELNMATDLFERKESPSWDFDEGEDRWRWMKPPPASKKA 540

Query: 541 VETE-DSASGKRSRRRIRQSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLE 600
           V+   D    K  R  I+ ++  ++R RLLKEF C IC++VM+ P+TTPCAHNFCK CLE
Sbjct: 541 VKNVLDPEERKLLREAIKSANPNTMRARLLKEFKCQICQKVMTNPVTTPCAHNFCKACLE 600

Query: 601 GAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIE 660
             F G   +RER SGGR LRSQK+VM CPCCPTDI++F+QN QVNRE+ +VIE LK + E
Sbjct: 601 SKFAGTALVRERGSGGRKLRSQKSVMKCPCCPTDIAEFVQNPQVNREVAEVIEKLKKQEE 654

Query: 661 EGDASSEKLCEEEIEGEEDEEEEEGKDGSSSPVETRKRAKAVV 683
           E +A S  L E +  G   EEE++ +      ++T     A V
Sbjct: 661 EENAKS--LDEGQCSGTSHEEEDDEQPKKRIKLDTDAEVSATV 654

BLAST of Spg007450 vs. ExPASy TrEMBL
Match: A0A6J1BYU6 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111006998 PE=4 SV=1)

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 613/709 (86.46%), Postives = 630/709 (88.86%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICMLC+AKPSDLETITCKTCVTPWHV CLSNPPETLASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMLCKAKPSDLETITCKTCVTPWHVACLSNPPETLASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S P  D PLPP      PTAPSS LVAAIRAIEADA LTDR+KAKKRQELL+G S S+KD
Sbjct: 61  SSPPGDVPLPP------PTAPSSHLVAAIRAIEADAFLTDRQKAKKRQELLTGNSHSDKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           D DA   K   GDDVL+LFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DRDATNRKSLIGDDVLELFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSI SGGPQKVYHFVHNQNRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIVSGGPQKVYHFVHNQNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQS+NGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSSNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPR LPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRALPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERKEGPSWDFDEKDSRWKWSK PPTSKKPVETEDSASGKRSRR++RQS 
Sbjct: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKPPPTSKKPVETEDSASGKRSRRKVRQSQ 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NMSVRE+LLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAFVGKTFLRERSSGGRALRS
Sbjct: 541 NMSVREKLLKEFSCLICREVMTLPITTPCAHNFCKSCLEGAFVGKTFLRERSSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEDEE 660
           QKNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGD +SEKLCEE  +G     
Sbjct: 601 QKNVMTCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGD-TSEKLCEEGTDG----- 660

Query: 661 EEEGKDGSSS-PVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
           EEEGK+ SS   VETRKRAK V +GGEEN V G+GDDSPSSPLHVDDLE
Sbjct: 661 EEEGKECSSPVGVETRKRAKVVADGGEENGVGGDGDDSPSSPLHVDDLE 660

BLAST of Spg007450 vs. ExPASy TrEMBL
Match: A0A6J1HS20 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465604 PE=4 SV=1)

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 614/716 (85.75%), Postives = 631/716 (88.13%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPP EDA LPPP + PLP  PS+DLV AIRAIEADASLTDREKA KRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVTAIRAIEADASLTDREKANKRQELLSGKSQSEKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDAN+   KRGDDVLDLFDERL CSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SGG QKVYH VHN NRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISGGSQKVYHVVHNLNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPRPLPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRPLPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERK+ PSWDFDEKDSRWKWSK PPTSKK VETEDS SGK+SRRR+RQSH
Sbjct: 481 IPELKKATDITERKKDPSWDFDEKDSRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSH 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM+VRERLLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS
Sbjct: 541 NMNVRERLLKEFSCLICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE---E 660
           +KNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE   E
Sbjct: 601 RKNVMACPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGE 660

Query: 661 DEEEEEGKDG-----SSSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
            E+EEEGKDG     SS PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 661 GEQEEEGKDGSNGNESSRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Spg007450 vs. ExPASy TrEMBL
Match: A0A6J1F0L5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441068 PE=4 SV=1)

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 614/720 (85.28%), Postives = 632/720 (87.78%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPP EDA LPPP + PLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDAN+   KRGDDVLDLFDERL CSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SGG QKVYH VHN NRPDKAYTTDR
Sbjct: 181 AKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISGGSQKVYHVVHNLNRPDKAYTTDR 240

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 241 AQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           KALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 361 KALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDNDPAPWT                                     SDEHGDRPRPLPA
Sbjct: 421 RCDNDPAPWT-------------------------------------SDEHGDRPRPLPA 480

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           IPELKKATDITERK+ PSWDFDEKD+RWKWSK PPTSKK VETEDS SGK+SRRR+RQSH
Sbjct: 481 IPELKKATDITERKKDPSWDFDEKDNRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSH 540

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM+VRERLLKEFSCLICREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS
Sbjct: 541 NMNVRERLLKEFSCLICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRS 600

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE---- 660
           +KNVM+CPCCPTDISDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE    
Sbjct: 601 RKNVMACPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGE 660

Query: 661 ---EDEEEEEGKDG-----SSSPVETRKRAKAVVNGGEENAVAGNGDDSPSSPLHVDDLE 709
              E E+EEEGKDG     SS PVETRKRAK VVNGGEEN        SPSSP+HVDDLE
Sbjct: 661 EEQEQEQEEEGKDGSNGNESSRPVETRKRAKVVVNGGEENY-------SPSSPVHVDDLE 672

BLAST of Spg007450 vs. ExPASy TrEMBL
Match: A0A6J1K0C8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489939 PE=4 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 583/680 (85.74%), Postives = 606/680 (89.12%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA  +QLPCDGDGICMLC+AKPSDLETITCKTCVTPWHV CLS+PPETLASTLQWDCPDC
Sbjct: 16  MASKTQLPCDGDGICMLCKAKPSDLETITCKTCVTPWHVDCLSSPPETLASTLQWDCPDC 75

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S P ED PLPP GN+PLPT PSSDLVAAIRAIEAD SLTDREKAKKRQELLSGK QS+KD
Sbjct: 76  SSPPEDVPLPPIGNNPLPTTPSSDLVAAIRAIEADVSLTDREKAKKRQELLSGKMQSDKD 135

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           DHDANKEK+K+GDDVLDL DERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 136 DHDANKEKKKKGDDVLDLLDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 195

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMASQPRINSTLVVAIRMAK+SKS+ SGGPQKVYHFVHNQNRPDKAYTTDR
Sbjct: 196 AKCRCVIPSKMASQPRINSTLVVAIRMAKLSKSLVSGGPQKVYHFVHNQNRPDKAYTTDR 255

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQ+ GKANAASGKIFVTIP+DHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 256 AQRNGKANAASGKIFVTIPTDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 315

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 316 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 375

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           +ALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIY+IEKCWRKVGIQGFKVCRYLFV
Sbjct: 376 QALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYKIEKCWRKVGIQGFKVCRYLFV 435

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDN+PAPWT                                     SDEHGDRPRPLP 
Sbjct: 436 RCDNEPAPWT-------------------------------------SDEHGDRPRPLPK 495

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           I ELKKATDITERK G SWDFDE DSRWKWSKAPPTSK+PVE EDSASGK  RR+IRQS 
Sbjct: 496 ISELKKATDITERKGGSSWDFDESDSRWKWSKAPPTSKRPVEAEDSASGK--RRKIRQSR 555

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NMSVRERLLKEFSC ICRE MSLPITTPCAHNFCK CLEG FVGKTFLRERSSGGRALRS
Sbjct: 556 NMSVRERLLKEFSCRICREAMSLPITTPCAHNFCKPCLEGVFVGKTFLRERSSGGRALRS 615

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEED-E 660
           QKNVM+CPCCPTDISDFLQN+QVNRELLDVIESLKSKIEEGDA SEKLCEE I+GEE+ +
Sbjct: 616 QKNVMNCPCCPTDISDFLQNIQVNRELLDVIESLKSKIEEGDA-SEKLCEEVIDGEEEGK 655

Query: 661 EEEEGKDGSSSPVETRKRAK 680
           +   G + SSS   TRKRAK
Sbjct: 676 DGSNGNESSSSVETTRKRAK 655

BLAST of Spg007450 vs. ExPASy TrEMBL
Match: A0A6J1EQ65 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111436380 PE=4 SV=1)

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 587/685 (85.69%), Postives = 605/685 (88.32%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA  +QLPCDGDGICMLC+AKPSDLETITCKTCVTPWHV CLS+P ETLASTLQWDCPDC
Sbjct: 16  MANKTQLPCDGDGICMLCKAKPSDLETITCKTCVTPWHVDCLSSPLETLASTLQWDCPDC 75

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S P ED PLPP GN+PLPT PSSDLVAAIRAIEAD SLTDREKAKKRQELLSGK QS+KD
Sbjct: 76  SSPPEDVPLPPLGNNPLPTTPSSDLVAAIRAIEADVSLTDREKAKKRQELLSGKLQSDKD 135

Query: 121 DHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 180
           D DANKEK+K+GDDVLDL DERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC
Sbjct: 136 DPDANKEKKKKGDDVLDLLDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGKNTC 195

Query: 181 AKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDR 240
           AKCRCVIPSKMASQPRINSTLVVAIRMAK+SKS+ SGGPQKVYHFVHNQNRPDKAYTTDR
Sbjct: 196 AKCRCVIPSKMASQPRINSTLVVAIRMAKLSKSLVSGGPQKVYHFVHNQNRPDKAYTTDR 255

Query: 241 AQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 300
           AQ+ GKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV
Sbjct: 256 AQRNGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHV 315

Query: 301 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 360
           AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN
Sbjct: 316 AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSN 375

Query: 361 KALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 420
           +ALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV
Sbjct: 376 QALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFV 435

Query: 421 RCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRPLPA 480
           RCDN+PAPWT                                     SDEHGDRPRPLP 
Sbjct: 436 RCDNEPAPWT-------------------------------------SDEHGDRPRPLPK 495

Query: 481 IPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIRQSH 540
           I ELKKATDITERK G SWDFDE DSRWKWS APPTSK+PVE EDSASGK  RR+IRQS 
Sbjct: 496 ISELKKATDITERKGGSSWDFDESDSRWKWSTAPPTSKRPVEAEDSASGK--RRKIRQSR 555

Query: 541 NMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRS 600
           NM VRERLLKEFSC ICREVMSLPITTPCAHNFCK CLEG FVGKTFLRERSSGGRALRS
Sbjct: 556 NMGVRERLLKEFSCRICREVMSLPITTPCAHNFCKPCLEGVFVGKTFLRERSSGGRALRS 615

Query: 601 QKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEDEE 660
           QKNVM+CPCCPTDISDFLQN+QVNRELLDVIESLKSKIE+GDA SEKLCEE I     +E
Sbjct: 616 QKNVMNCPCCPTDISDFLQNIQVNRELLDVIESLKSKIEDGDA-SEKLCEEVI-----DE 655

Query: 661 EEEGKDG-----SSSPVE-TRKRAK 680
           EEEGKDG     SSS VE TRKRAK
Sbjct: 676 EEEGKDGSTGNESSSSVETTRKRAK 655

BLAST of Spg007450 vs. TAIR 10
Match: AT1G57820.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 862.8 bits (2228), Expect = 1.9e-250
Identity = 436/667 (65.37%), Postives = 503/667 (75.41%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA+  QLPCDGDG+CM C++ P   E++TC TCVTPWHV CLS+PP+TLASTLQW CPDC
Sbjct: 1   MARDIQLPCDGDGVCMRCKSNPPPEESLTCGTCVTPWHVSCLSSPPKTLASTLQWHCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S   E  PLP  G +    +  SDLVAAIRAIEAD SL+  EKAK RQ LLSGK   E D
Sbjct: 61  S--GEIDPLPVSGGATGFESAGSDLVAAIRAIEADESLSTEEKAKMRQRLLSGKGVEEDD 120

Query: 121 DHDANKEKRKRGD---DVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGK 180
           + +  K+K K  +   DVL    + L CSFCMQLPERPVT PCGHN CLKCF+KW+GQGK
Sbjct: 121 EEEKRKKKGKGKNPNLDVLSALGDNLMCSFCMQLPERPVTKPCGHNACLKCFEKWMGQGK 180

Query: 181 NTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYT 240
            TC KCR +IP KMA  PRINS+LV AIR+AK+SKS  +    KV+HF+ NQ+RPDKA+T
Sbjct: 181 RTCGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKS-AAATTSKVFHFISNQDRPDKAFT 240

Query: 241 TDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHF 300
           T+RA+K GKANAASGKI+VTIP DHFGPIPAENDP RNQG+LVGE WEDRLECRQWGAHF
Sbjct: 241 TERAKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGLLVGESWEDRLECRQWGAHF 300

Query: 301 PHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFE 360
           PHVAGIAGQS  GAQSV LSGGY+DDEDHGEWFLYTGSGGRDLSGNKRT+K+QSFDQKFE
Sbjct: 301 PHVAGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGRDLSGNKRTNKEQSFDQKFE 360

Query: 361 KSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQG-FKVCR 420
           KSN AL++SC  GYPVRVVRSHKEKRS+YAPE+G+RYDG+YRIEKCWRKVG+QG FKVCR
Sbjct: 361 KSNAALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVYRIEKCWRKVGVQGSFKVCR 420

Query: 421 YLFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPR 480
           YLFVRCDN+PAPWT                                     SDE+GDRPR
Sbjct: 421 YLFVRCDNEPAPWT-------------------------------------SDENGDRPR 480

Query: 481 PLPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRI 540
           P+P IPEL  ATD+ ERKE PSWDFDE +  WKW K PP SKK V        K  R+ I
Sbjct: 481 PIPNIPELNMATDLFERKETPSWDFDEGEGCWKWMKPPPASKKSVNVLAPEERKNLRKAI 540

Query: 541 RQSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGR 600
           + +H+ ++R RLLKEF C IC++V++LP+TTPCAHNFCK CLE  F GKT +RERS+GGR
Sbjct: 541 KAAHSNTMRARLLKEFKCQICQQVLTLPVTTPCAHNFCKACLEAKFAGKTLVRERSTGGR 600

Query: 601 ALRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE 660
            LRS+KNV++CPCCPTDISDFLQN QVNRE+ +VIE LK++ EE  A  E   E E  G 
Sbjct: 601 TLRSRKNVLNCPCCPTDISDFLQNPQVNREVAEVIEKLKTQ-EEDTAELEDEDEGECSGT 626

Query: 661 EDEEEEE 664
             EE+ E
Sbjct: 661 TPEEDSE 626

BLAST of Spg007450 vs. TAIR 10
Match: AT1G57820.2 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 852.0 bits (2200), Expect = 3.3e-247
Identity = 433/667 (64.92%), Postives = 500/667 (74.96%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA+  QLPCDGDG+CM C++ P   E++TC TCVTPWHV CLS+PP+TLASTLQW CPDC
Sbjct: 1   MARDIQLPCDGDGVCMRCKSNPPPEESLTCGTCVTPWHVSCLSSPPKTLASTLQWHCPDC 60

Query: 61  SPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S   E  PLP  G +    +  SDLVAAIRAIEAD SL+  EKAK RQ LLSGK   E D
Sbjct: 61  S--GEIDPLPVSGGATGFESAGSDLVAAIRAIEADESLSTEEKAKMRQRLLSGKGVEEDD 120

Query: 121 DHDANKEKRKRGD---DVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKWIGQGK 180
           + +  K+K K  +   DVL    + L CSFCMQLPERPVT PCGHN CLKCF+KW+GQGK
Sbjct: 121 EEEKRKKKGKGKNPNLDVLSALGDNLMCSFCMQLPERPVTKPCGHNACLKCFEKWMGQGK 180

Query: 181 NTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYT 240
            TC KCR +IP KMA  PRINS+LV AIR+AK+SKS  +    KV+HF+ NQ+RPDKA+T
Sbjct: 181 RTCGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKS-AAATTSKVFHFISNQDRPDKAFT 240

Query: 241 TDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHF 300
           T+RA+K GKANAASGKI+VTIP DHFGPIPAENDP RNQG+LVGE WEDRLECRQWGAHF
Sbjct: 241 TERAKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGLLVGESWEDRLECRQWGAHF 300

Query: 301 PHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFE 360
           PHVAGIAGQS  GAQSV LSGGY+DDEDHGEWFLYT   GRDLSGNKRT+K+QSFDQKFE
Sbjct: 301 PHVAGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYT---GRDLSGNKRTNKEQSFDQKFE 360

Query: 361 KSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQG-FKVCR 420
           KSN AL++SC  GYPVRVVRSHKEKRS+YAPE+G+RYDG+YRIEKCWRKVG+QG FKVCR
Sbjct: 361 KSNAALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVYRIEKCWRKVGVQGSFKVCR 420

Query: 421 YLFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPR 480
           YLFVRCDN+PAPWT                                     SDE+GDRPR
Sbjct: 421 YLFVRCDNEPAPWT-------------------------------------SDENGDRPR 480

Query: 481 PLPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRI 540
           P+P IPEL  ATD+ ERKE PSWDFDE +  WKW K PP SKK V        K  R+ I
Sbjct: 481 PIPNIPELNMATDLFERKETPSWDFDEGEGCWKWMKPPPASKKSVNVLAPEERKNLRKAI 540

Query: 541 RQSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGR 600
           + +H+ ++R RLLKEF C IC++V++LP+TTPCAHNFCK CLE  F GKT +RERS+GGR
Sbjct: 541 KAAHSNTMRARLLKEFKCQICQQVLTLPVTTPCAHNFCKACLEAKFAGKTLVRERSTGGR 600

Query: 601 ALRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE 660
            LRS+KNV++CPCCPTDISDFLQN QVNRE+ +VIE LK++ EE  A  E   E E  G 
Sbjct: 601 TLRSRKNVLNCPCCPTDISDFLQNPQVNREVAEVIEKLKTQ-EEDTAELEDEDEGECSGT 623

Query: 661 EDEEEEE 664
             EE+ E
Sbjct: 661 TPEEDSE 623

BLAST of Spg007450 vs. TAIR 10
Match: AT5G39550.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 765.4 bits (1975), Expect = 4.1e-221
Identity = 399/672 (59.38%), Postives = 476/672 (70.83%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST +W+CPD
Sbjct: 1   MAIETQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVPCLL--PESLASSTGEWECPD 60

Query: 61  CSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  GN+  P +  S LVAAIRAI+AD +LT+ EKAKKRQ+L+SG      
Sbjct: 61  CSGVVVPSAAPGTGNA-RPESSGSVLVAAIRAIQADETLTEAEKAKKRQKLMSGGG---- 120

Query: 121 DDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKW-IGQGKN 180
           DD    +EK+K     L++F     CS C+QLPERP+TTPCGHNFCLKCF+KW +GQGK 
Sbjct: 121 DDGVDEEEKKK-----LEIF-----CSICIQLPERPITTPCGHNFCLKCFEKWAVGQGKL 180

Query: 181 TCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTT 240
           TC  CR  IP  +A  PRIN  LV AIR+A ++K        KV+H + NQ+RP+KA+TT
Sbjct: 181 TCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSVEATAAKVHHIIRNQDRPEKAFTT 240

Query: 241 DRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFP 300
           +RA K GKANAASGK FVTIP DHFGPIPAEND  R QGVLVGE WEDR ECRQWGAHFP
Sbjct: 241 ERAVKTGKANAASGKFFVTIPRDHFGPIPAENDVTRKQGVLVGESWEDRQECRQWGAHFP 300

Query: 301 HVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEK 360
           H+AGIAGQS  GAQSV LSGGY DDEDHGEWFLYTGSGGRDLSGNKR +K QS DQ F+ 
Sbjct: 301 HIAGIAGQSAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRINKKQSSDQAFKN 360

Query: 361 SNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQG-FKVCRY 420
            N++L++SC  GYPVRVVRS KEKRS+YAP +G+RYDG+YRIEKCW  VG+QG FKVCRY
Sbjct: 361 MNESLRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGSFKVCRY 420

Query: 421 LFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRP 480
           LFVRCDN+PAPWT                                     SDEHGDRPRP
Sbjct: 421 LFVRCDNEPAPWT-------------------------------------SDEHGDRPRP 480

Query: 481 LPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIR 540
           LP +PEL+ A D+  RKE PSWDFDE + RWKW K+PP S+  ++ E+    KR++    
Sbjct: 481 LPNVPELETAADLFVRKESPSWDFDEAEGRWKWMKSPPVSRMALDPEERKKNKRAKN--- 540

Query: 541 QSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRA 600
                +++ RLLKEFSC ICREV+SLP+TTPCAHNFCK CLE  F G T LRERS+GGR 
Sbjct: 541 -----TMKARLLKEFSCQICREVLSLPVTTPCAHNFCKACLEAKFAGITQLRERSNGGRK 600

Query: 601 LRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEE 660
           LR++KN+M+CPCC TD+S+FLQN QVNRE++++IE+ K   EE DAS   + EEE E  E
Sbjct: 601 LRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEADAS---ISEEEEEESE 607

Query: 661 DEEEEEGKDGSS 670
              ++   D +S
Sbjct: 661 PPTKKIKMDNNS 607

BLAST of Spg007450 vs. TAIR 10
Match: AT1G66050.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 761.5 bits (1965), Expect = 5.9e-220
Identity = 395/663 (59.58%), Postives = 469/663 (70.74%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST  W+CPD
Sbjct: 1   MAIQTQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVSCLL--PESLASSTGDWECPD 60

Query: 61  CSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  G S  P +  S LV AIRAI+AD +LT+ EKAKKRQ L+SG      
Sbjct: 61  CSGVVVPSAAPGTGISG-PESSGSVLVTAIRAIQADVTLTEAEKAKKRQRLMSGGGDDGV 120

Query: 121 DDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKW-IGQGKN 180
           DD    +EK+K     L++F     CS C+QLPERPVTTPCGHNFCLKCF+KW +GQGK 
Sbjct: 121 DD----EEKKK-----LEIF-----CSICIQLPERPVTTPCGHNFCLKCFEKWAVGQGKL 180

Query: 181 TCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTT 240
           TC  CR  IP  +A  PRIN  LV AIR+A ++K  G     KV+H + NQ+RPDKA+TT
Sbjct: 181 TCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGEATAAKVHHIIRNQDRPDKAFTT 240

Query: 241 DRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFP 300
           +RA K GKANAASGK FVTIP DHFGPIPA ND  RNQGVLVGE WEDR ECRQWG HFP
Sbjct: 241 ERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQGVLVGESWEDRQECRQWGVHFP 300

Query: 301 HVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEK 360
           HVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSGGRDLSGNKR +K QS DQ F+ 
Sbjct: 301 HVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRVNKIQSSDQAFKN 360

Query: 361 SNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGF-KVCRY 420
            N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG+YRIEKCW  VG+QG  K+CRY
Sbjct: 361 MNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGLHKMCRY 420

Query: 421 LFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRP 480
           LFVRCDN+PAPWT                                     SDEHGDRPRP
Sbjct: 421 LFVRCDNEPAPWT-------------------------------------SDEHGDRPRP 480

Query: 481 LPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIR 540
           LP +PEL+ ATD+  RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++   
Sbjct: 481 LPDVPELENATDLFVRKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK--- 540

Query: 541 QSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRA 600
              N +++ RLLKEFSC ICR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R 
Sbjct: 541 --GNNAMKARLLKEFSCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRK 600

Query: 601 LRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEE 660
           LR++KN+M+CPCC TD+S+FLQN QVNRE++++IE+ K   EE + +      EE EGEE
Sbjct: 601 LRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEE-EGEE 603

BLAST of Spg007450 vs. TAIR 10
Match: AT1G66040.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 759.2 bits (1959), Expect = 2.9e-219
Identity = 398/673 (59.14%), Postives = 474/673 (70.43%), Query Frame = 0

Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST  W+CPD
Sbjct: 1   MAIQTQLPCDGDGVCMRCQVTPPSEETLTCGTCVTPWHVSCLL--PESLASSTGDWECPD 60

Query: 61  CSPPREDAPLPPPGNSPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  G S  P +  S LVAAIRAI+AD +LT+ EKAKKRQ L+SG      
Sbjct: 61  CSGVVVPSAAPGTGISG-PESSGSVLVAAIRAIQADVTLTEAEKAKKRQRLMSGGGDDGV 120

Query: 121 DDHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTTPCGHNFCLKCFQKW-IGQGKN 180
           DD    +EK+K     L++F     CS C+QLPERPVTTPCGHNFCLKCF+KW +GQGK 
Sbjct: 121 DD----EEKKK-----LEIF-----CSICIQLPERPVTTPCGHNFCLKCFEKWAVGQGKL 180

Query: 181 TCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTT 240
           TC  CR  IP  +A  PRIN  LV AIR+A ++K  G     KV+H + NQ+RPDKA+TT
Sbjct: 181 TCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGEATAAKVHHIIRNQDRPDKAFTT 240

Query: 241 DRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFP 300
           +RA K GKANAASGK FVTIP DHFGPIPA ND  RNQGVLVGE WEDR ECRQWG HFP
Sbjct: 241 ERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQGVLVGESWEDRQECRQWGVHFP 300

Query: 301 HVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEK 360
           HVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSGGRDLSGNKR +K QS DQ F+ 
Sbjct: 301 HVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSGGRDLSGNKRVNKIQSSDQAFKN 360

Query: 361 SNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGF-KVCRY 420
            N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG+YRIEKCW  VG+QG  K+CRY
Sbjct: 361 MNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDGVYRIEKCWSNVGVQGLHKMCRY 420

Query: 421 LFVRCDNDPAPWTRFVFPNNVIAFVLSNASCSLWLCLQSFFLSLNKLFNYSDEHGDRPRP 480
           LFVRCDN+PAPWT                                     SDEHGDRPRP
Sbjct: 421 LFVRCDNEPAPWT-------------------------------------SDEHGDRPRP 480

Query: 481 LPAIPELKKATDITERKEGPSWDFDEKDSRWKWSKAPPTSKKPVETEDSASGKRSRRRIR 540
           LP +PEL+ ATD+  RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++   
Sbjct: 481 LPDVPELENATDLFVRKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK--- 540

Query: 541 QSHNMSVRERLLKEFSCLICREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRA 600
              N +++ RLLKEFSC ICR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R 
Sbjct: 541 --GNNAMKARLLKEFSCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRK 600

Query: 601 LRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGD-ASSEKLCEEEIEGE 660
           LR++KN+M+CPCC TD+S+FLQN QVNRE++++IE+ K   EE + A S  + EEE E  
Sbjct: 601 LRAKKNIMTCPCCTTDLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEEEEEES 614

Query: 661 EDEEEEEGKDGSS 670
           E   ++   D +S
Sbjct: 661 EPPTKKIKMDNNS 614

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134816.10.0e+0086.46E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Momordica charantia][more]
XP_023531327.10.0e+0085.89E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita pepo subsp. pepo][more]
XP_022965844.10.0e+0085.75E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita maxima][more]
XP_022933744.10.0e+0085.28E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita moschata][more]
KAG6587385.10.0e+0085.61E3 ubiquitin-protein ligase ORTHRUS 2, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
Q8VYZ02.6e-24965.37E3 ubiquitin-protein ligase ORTHRUS 2 OS=Arabidopsis thaliana OX=3702 GN=ORTH2 P... [more]
Q9FKA75.7e-22059.38E3 ubiquitin-protein ligase ORTHRUS 1 OS=Arabidopsis thaliana OX=3702 GN=ORTH1 P... [more]
Q680I08.3e-21959.58E3 ubiquitin-protein ligase ORTHRUS 5 OS=Arabidopsis thaliana OX=3702 GN=ORTH5 P... [more]
Q9C8E14.1e-21859.14Putative E3 ubiquitin-protein ligase ORTHRUS 4 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FVS25.2e-21355.76E3 ubiquitin-protein ligase ORTHRUS 3 OS=Arabidopsis thaliana OX=3702 GN=ORTH3 P... [more]
Match NameE-valueIdentityDescription
A0A6J1BYU60.0e+0086.46RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11100699... [more]
A0A6J1HS200.0e+0085.75RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465604 P... [more]
A0A6J1F0L50.0e+0085.28RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441068... [more]
A0A6J1K0C80.0e+0085.74RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489939 P... [more]
A0A6J1EQ650.0e+0085.69RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111436380... [more]
Match NameE-valueIdentityDescription
AT1G57820.11.9e-25065.37Zinc finger (C3HC4-type RING finger) family protein [more]
AT1G57820.23.3e-24764.92Zinc finger (C3HC4-type RING finger) family protein [more]
AT5G39550.14.1e-22159.38Zinc finger (C3HC4-type RING finger) family protein [more]
AT1G66050.15.9e-22059.58Zinc finger (C3HC4-type RING finger) family protein [more]
AT1G66040.12.9e-21959.14Zinc finger (C3HC4-type RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003105SRA-YDGSMARTSM00466G9a_1coord: 268..425
e-value: 1.8E-75
score: 266.7
IPR003105SRA-YDGPFAMPF02182SAD_SRAcoord: 276..424
e-value: 1.1E-53
score: 181.1
IPR003105SRA-YDGPROSITEPS51015YDGcoord: 273..421
score: 52.585644
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 14..61
e-value: 6.0E-5
score: 32.5
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 145..183
e-value: 3.1E-6
score: 36.7
coord: 554..610
e-value: 1.6E-6
score: 37.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 554..610
score: 10.512376
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 145..184
score: 11.604098
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 554..608
e-value: 5.0E-8
score: 32.8
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 145..183
e-value: 4.6E-8
score: 32.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 507..642
e-value: 6.8E-17
score: 63.2
coord: 93..212
e-value: 7.1E-20
score: 72.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 2..70
e-value: 1.5E-6
score: 29.7
IPR036987SRA-YDG superfamilyGENE3D2.30.280.10coord: 244..435
e-value: 1.7E-77
score: 261.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 495..537
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..78
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 495..512
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 642..708
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 645..666
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..82
NoneNo IPR availablePANTHERPTHR14140:SF42FINGER PROTEIN, PUTATIVE-RELATEDcoord: 1..431
coord: 468..660
NoneNo IPR availableCDDcd16449RING-HCcoord: 145..183
e-value: 2.17023E-9
score: 51.3141
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 547..635
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 136..191
IPR045134UHRF1/2-likePANTHERPTHR14140E3 UBIQUITIN-PROTEIN LIGASE UHRF-RELATEDcoord: 1..431
coord: 468..660
IPR019786Zinc finger, PHD-type, conserved sitePROSITEPS01359ZF_PHD_1coord: 15..60
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 569..578
IPR019787Zinc finger, PHD-fingerPROSITEPS50016ZF_PHD_2coord: 12..63
score: 8.5644
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 247..435
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 7..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg007450.1Spg007450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006325 chromatin organization
biological_process GO:0010216 maintenance of DNA methylation
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity