Spg007229 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg007229 vs. NCBI nr
Match: KAG7024112.1 (putative acyl-activating enzyme 1, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1030.0 bits (2662), Expect = 7.8e-297
Identity = 508/584 (86.99%), Postives = 532/584 (91.10%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANY+PLTPITFLERSAAVYGDRISLVYGRVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVTGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PSHI+  GSASG LEFESLL SGKL+F+IRRPKDE DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSHIDREGSASGYLEFESLLESGKLDFKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRN +AKEIFDNI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLPGKVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL+SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. NCBI nr
Match: XP_022937090.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1030.0 bits (2662), Expect = 7.8e-297
Identity = 508/584 (86.99%), Postives = 532/584 (91.10%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANY+PLTPITFLERSAAVYGDRISLVYGRVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVMGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PSHI+  GSASG LEFESLL SGKL+F+IRRPKDE DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSHIDREGSASGYLEFESLLESGKLDFKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRN +AKEIFDNI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLPGKVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL+SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. NCBI nr
Match: KAG6591226.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1028.1 bits (2657), Expect = 3.0e-296
Identity = 507/584 (86.82%), Postives = 531/584 (90.92%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANY+PLTPITFLERSAAVYGDRISLVYGRVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVTGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PSHI+  GSASG LEFE LL SGKL+F+IRRPKDE DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSHIDREGSASGYLEFERLLESGKLDFKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRN +AKEIFDNI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLPGKVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL+SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. NCBI nr
Match: XP_023536083.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.5e-295
Identity = 507/584 (86.82%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANY+PLTPITFL+RSAAVYGDRISLVY RVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYLPLTPITFLQRSAAVYGDRISLVYERVQYTWRETLFRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVTGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PSHI+  GSASG LEFESLL SGKL+F+IRRPKDE DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSHIDIEGSASGYLEFESLLESGKLDFKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRN TAKEIFDNI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNATAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLPGKVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLTSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. NCBI nr
Match: XP_022974850.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.5e-292
Identity = 503/584 (86.13%), Postives = 527/584 (90.24%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANYVPLTPITFLERSAAVYGDRISL YGRVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLAYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVMGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PS+I+  GSAS  LEFESLL SGKL+F+IRRPK E DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSYIDREGSASEYLEFESLLESGKLDFKIRRPKYELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRNV+AKEIF+NI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNVSAKEIFENI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLP KVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPRKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLTSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDD WGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDRWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 5.8e-226
Identity = 374/580 (64.48%), Postives = 457/580 (78.79%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           M+G  +  ANYVPLTPI+FL+RSA VY DR+S+VYG V+YTWR+T  RC R+ASAL ++G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           I+ GDV                             V+ LAPN+PAM ELHF VPMAGA+L
Sbjct: 63  ISTGDV-----------------------------VSVLAPNVPAMVELHFGVPMAGALL 122

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           CTLN+RHD+++V+ LL HS  K+I  D+QF  I +GA +ILS + +K+P++V+I E    
Sbjct: 123 CTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKGDKVPILVLIPE--PL 182

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
              ++    +   +E+E ++A GK +FE+ RP DE D I++NYTSGTTS PKGV+YSHRG
Sbjct: 183 TQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGTTSSPKGVVYSHRG 242

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLNSL+AVLLN+M S P YLWT PMFHCNGWCL W V A GGTNIC RNVTAK IFDNI
Sbjct: 243 AYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNI 302

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           S HKVTHMGGAPT+LNMIINAP SEQKPLPGKV+ +TG APPP+HV++KM  +GF + H+
Sbjct: 303 SQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHVIFKMEELGFSMFHS 362

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGP T+C+WKPEWDSL  + QAK+ +RQG+ H+GLE++ +KDPVTM + PADG 
Sbjct: 363 YGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGV 422

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           TMGEV+FRGNTVM+GYLK+ +ATKEAF GGWF SGDLGV+HPDGYIELKDRSKDIIISGG
Sbjct: 423 TMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYIELKDRSKDIIISGG 482

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES LF+HP VLEAAVV RPD++WGET CAFVKLKDG  AS EE+I++C+  L
Sbjct: 483 ENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRL 542

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKR 581
           PHYMAPRS+VF+DLPKTSTGK QKF+L+ +AKA+ SL K+
Sbjct: 543 PHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLSKK 551

BLAST of Spg007229 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 9.9e-210
Identity = 357/585 (61.03%), Postives = 438/585 (74.87%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           ++G+ R  AN+ PL+PITFLERSA VY DR SLV+G V++TW +T QRC RLASAL  +G
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           I+RGDV                             VAALAPN+PAM+ELHFAVPMAG +L
Sbjct: 108 ISRGDV-----------------------------VAALAPNVPAMHELHFAVPMAGLIL 167

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILS---ERREKLPLVVIIQES 180
           C LN R D + +S LL HSEAK++ VD+Q   I  GA+ +L+   + R+ L LV+I Q +
Sbjct: 168 CPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLAKSDKTRKSLKLVLISQSN 227

Query: 181 DQPPSHING----AGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKG 240
           D   S  +     A   S D E+E+LL SG   FEI +P+ EWDPI++NYTSGTTSRPKG
Sbjct: 228 DDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCEWDPISINYTSGTTSRPKG 287

Query: 241 VIYSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTA 300
           V+YSHRGAYLNSL+ V L+ M   PVYLWTVPMFHCNGWCL W VAAQGGTNIC R V+ 
Sbjct: 288 VVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSP 347

Query: 301 KEIFDNISLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSM 360
           K IF NI++HKVTHMGGAPTVLNMI+N  ++E KPLP +V +MTGG+PP   +L KM  +
Sbjct: 348 KMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEEL 407

Query: 361 GFFIVHAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTME 420
           GF + H YGLTETYGP T C WKPEWDSLS + + KL +RQG+QH+GLE +D+KDP+TME
Sbjct: 408 GFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTME 467

Query: 421 SAPADGKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSK 480
           + P DG TMGEVMFRGNTVMSGY KD++AT++AF G WF SGDL V++PDGYIE+KDR K
Sbjct: 468 TVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLK 527

Query: 481 DIIISGGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGC-SASEEEI 540
           D+IISGGENISS+EVE +L SH +VLEAAVV RPD HWG+TPC FVKLK+G  +   EEI
Sbjct: 528 DVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEI 587

Query: 541 INFCKKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSL 578
           I FC+ HLPHYMAP+++VF D+PKTSTGK QK++L+++A  MGSL
Sbjct: 588 IGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEMGSL 603

BLAST of Spg007229 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 3.5e-199
Identity = 343/581 (59.04%), Postives = 429/581 (73.84%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASAL-VRM 60
           M+G+ +CSAN+VPL+PITFLERS+  Y D  SLVYG V+YTW +T  RC +LASAL   +
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  GIARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAV 120
           GI+ GDV                             VA  + N+P +YELHFAVPMAG +
Sbjct: 89  GISPGDV-----------------------------VATFSYNLPEIYELHFAVPMAGGI 148

Query: 121 LCTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQ 180
           LCTLN R+D+AMVSTLL HSEAK+I V+ Q     + A+ +L+++  K P +V++ +S+ 
Sbjct: 149 LCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLLAQKDIKPPTLVLLTDSE- 208

Query: 181 PPSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHR 240
                  + ++S    +  LLA+G  +FEIRRPK+EWDPI++NYTSGTT+RPK V+YSHR
Sbjct: 209 -------SFTSSSYDHYNHLLANGSDDFEIRRPKNEWDPISINYTSGTTARPKAVVYSHR 268

Query: 241 GAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDN 300
           GAYLNS++ VLL+ M +  VYLW+VPMFHCNGWC  W  AAQG TNIC R V+ K IFDN
Sbjct: 269 GAYLNSIATVLLHGMGTTSVYLWSVPMFHCNGWCFPWGAAAQGATNICIRKVSPKAIFDN 328

Query: 301 ISLHKVTHMGGAPTVLNMIINAPISE-QKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIV 360
           I LHKVTH G APTVLNMI+N+P      PLP KV +MTGG+PPP  V+ +M  MGF + 
Sbjct: 329 IHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVN 388

Query: 361 HAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPAD 420
           H YGLTET GPA  C  KPEWD+L  + +  L +RQGL H+ +E++D++DPVTMES  AD
Sbjct: 389 HIYGLTETCGPAANCVCKPEWDALQPEERYALKARQGLNHLAMEEMDVRDPVTMESVRAD 448

Query: 421 GKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIIS 480
           G T+GEVMFRGNTVMSGY KDLKAT+EAF GGWFRSGDLGV+H DGYI+LKDR KD++IS
Sbjct: 449 GATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRSGDLGVKHEDGYIQLKDRKKDVVIS 508

Query: 481 GGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGC--SASEEEIINFC 540
           GGENIS++EVE++L+SH +VLEAAVV RPD  WGETPCAFV LK+G     S ++II FC
Sbjct: 509 GGENISTVEVETVLYSHEAVLEAAVVARPDKLWGETPCAFVTLKEGFDNDVSADQIIKFC 568

Query: 541 KKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSL 578
           +  LPHYMAP++VVF++LPKTSTGK QK+ILKE+A AMGSL
Sbjct: 569 RDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAMAMGSL 572

BLAST of Spg007229 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.3e-198
Identity = 342/581 (58.86%), Postives = 430/581 (74.01%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASAL-VRM 60
           M+G+ +CSAN+VPL+PITFLERS+  Y D  SLVYG V+YTW +T  RC +LASAL    
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  GIARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAV 120
           GI+ GDV                             VA  + NIP +YELHFAVPMAG +
Sbjct: 89  GISPGDV-----------------------------VATFSYNIPEIYELHFAVPMAGGI 148

Query: 121 LCTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQ 180
           LCTLN R+D+AMVSTLL HSEAK+I V+ Q     + A+ +L+++  K P +V++ +S+ 
Sbjct: 149 LCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLLAQKDIKPPTLVLLTDSE- 208

Query: 181 PPSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHR 240
                  + ++S    +  LLA+G  +FEIRRPK+E DPI++NYTSGTT+RPK V+YSHR
Sbjct: 209 -------SFTSSSYDHYNHLLANGSDDFEIRRPKNECDPISINYTSGTTARPKAVVYSHR 268

Query: 241 GAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDN 300
           GAYLNS++ VLL+ M +  VYLW+VPMFHCNGWC  W  AAQG TNIC R V+ K IFDN
Sbjct: 269 GAYLNSIATVLLHGMGTRSVYLWSVPMFHCNGWCFPWGAAAQGATNICIRKVSPKAIFDN 328

Query: 301 ISLHKVTHMGGAPTVLNMIINAPISE-QKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIV 360
           I LHKVTH G APTVLNMI+N+P      PLP KV +MTGG+PPP  V+ +M  MGF + 
Sbjct: 329 IHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVN 388

Query: 361 HAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPAD 420
           H YGLTET+GPAT C  KPEWD+L  + +  L +RQGL H+ +E++D++DPV+MES  AD
Sbjct: 389 HIYGLTETHGPATNCVCKPEWDALQPEERYALKARQGLNHLAMEEMDVRDPVSMESVRAD 448

Query: 421 GKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIIS 480
           G T+GEVMFRGNTVMSGY KDLKAT+EAF GGWFR+GDLGV+H DGYI+LKDR KD++IS
Sbjct: 449 GTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRTGDLGVKHEDGYIQLKDRKKDVVIS 508

Query: 481 GGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGC--SASEEEIINFC 540
           GGEN+S++EVE++L+SH +VLEAAVV RPD  WGETPCAFV LK+G     S ++II FC
Sbjct: 509 GGENVSTVEVETVLYSHEAVLEAAVVARPDKLWGETPCAFVTLKEGFDNGVSADQIIKFC 568

Query: 541 KKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSL 578
           +  LPHYMAP++VVF++LPKTSTGK QK+ILKE+AKAMGSL
Sbjct: 569 RDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAKAMGSL 572

BLAST of Spg007229 vs. ExPASy Swiss-Prot
Match: Q8VZF1 (Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE7 PE=1 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 4.2e-168
Identity = 294/588 (50.00%), Postives = 394/588 (67.01%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           +D + +  ANY  LTP+ FL+R+A V+  R S+++G  +YTWR+T  RC RLASAL    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           I  G                               VA +APNIPAMYE HF VPM GAVL
Sbjct: 69  IGPGST-----------------------------VAIIAPNIPAMYEAHFGVPMCGAVL 128

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERRE---KLPLVVIIQES 180
             +N+R +A  V+ LL+HS++ +I+VD +F  + + +++++ E+     K PL+++I + 
Sbjct: 129 NCVNIRLNAPTVAFLLSHSQSSVIMVDQEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDH 188

Query: 181 DQPPSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYS 240
              P  +N A S  G +E+E  LA+G  N+  + P DEW  IAL YTSGTT+ PKGV+  
Sbjct: 189 TCAPESLNRALS-KGAIEYEDFLATGDPNYPWQPPADEWQSIALGYTSGTTASPKGVVLH 248

Query: 241 HRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIF 300
           HRGAY+ +LS  L+  M    VYLWT+PMFHCNGWC  W++A   GT+IC R VTAKE++
Sbjct: 249 HRGAYIMALSNPLIWGMQDGAVYLWTLPMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVY 308

Query: 301 DNISLHKVTHMGGAPTVLNMIINAPISEQ-KPLPGKVTMMTGGAPPPSHVLYKMRSMGFF 360
             I+ +KVTH   AP VLN I+NAP  +   PLP  V +MT GA PP  VL+ M   GF 
Sbjct: 309 SMIAKYKVTHFCAAPVVLNAIVNAPKEDTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFR 368

Query: 361 IVHAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAP 420
           + H YGL+ETYGP+TVC+WKPEWDSL  + QAKLN+RQG+++ G+E +D+ D  T +  P
Sbjct: 369 VAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNARQGVRYTGMEQLDVIDTQTGKPVP 428

Query: 421 ADGKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDII 480
           ADGKT GE++FRGN VM GYLK+ +A KE F GGWF SGD+ V+HPD YIE+KDRSKD+I
Sbjct: 429 ADGKTAGEIVFRGNMVMKGYLKNPEANKETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVI 488

Query: 481 ISGGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASE-----EE 540
           ISGGENISS+EVE++++ HP+VLEA+VV RPD+ W E+PCAFV LK      +     ++
Sbjct: 489 ISGGENISSVEVENVVYHHPAVLEASVVARPDERWQESPCAFVTLKSDYEKHDQNKLAQD 548

Query: 541 IINFCKKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK 580
           I+ FC++ LP Y  P+SVVF  LPKT+TGK QK IL+ +AK MG +P+
Sbjct: 549 IMKFCREKLPAYWVPKSVVFGPLPKTATGKIQKHILRTKAKEMGPVPR 566

BLAST of Spg007229 vs. ExPASy TrEMBL
Match: A0A6J1FFJ8 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111443496 PE=4 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 3.8e-297
Identity = 508/584 (86.99%), Postives = 532/584 (91.10%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANY+PLTPITFLERSAAVYGDRISLVYGRVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVMGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PSHI+  GSASG LEFESLL SGKL+F+IRRPKDE DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSHIDREGSASGYLEFESLLESGKLDFKIRRPKDELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRN +AKEIFDNI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNASAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLPGKVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPGKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL+SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLSSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. ExPASy TrEMBL
Match: A0A6J1IF13 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111473618 PE=4 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.2e-292
Identity = 503/584 (86.13%), Postives = 527/584 (90.24%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDGMDRCSANYVPLTPITFLERSAAVYGDRISL YGRVQYTWRETL RCTRLASALVRMG
Sbjct: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLAYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN+RHDAAMVSTLL+HSEAK+IVVDYQFEHIV GAIK +SERREK+PLVVII E DQP
Sbjct: 121 CSLNVRHDAAMVSTLLSHSEAKIIVVDYQFEHIVMGAIKAMSERREKIPLVVIINEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PS+I+  GSAS  LEFESLL SGKL+F+IRRPK E DPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSYIDREGSASEYLEFESLLESGKLDFKIRRPKYELDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLW VPMFHCNGW LTW VAAQ GTNICQRNV+AKEIF+NI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWVVPMFHCNGWSLTWGVAAQSGTNICQRNVSAKEIFENI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTVLNMIINAP SEQKPLP KVTMMTGGAPPPSHVL+KMR+MGF IVHA
Sbjct: 301 SLHKVTHMGGAPTVLNMIINAPTSEQKPLPRKVTMMTGGAPPPSHVLFKMRAMGFLIVHA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKL SRQGLQH+GLE+VDIKDPVTMESAPADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLTSRQGLQHVGLEEVDIKDPVTMESAPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+GEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG
Sbjct: 421 TIGEVMVRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHPSVLEAAVVGRPDD WGETPCAFVKLKDGCSASEE+II FC++HL
Sbjct: 481 ENISSIEVESVLFSHPSVLEAAVVGRPDDRWGETPCAFVKLKDGCSASEEDIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK+ SKL
Sbjct: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKQTSKL 555

BLAST of Spg007229 vs. ExPASy TrEMBL
Match: A0A5D3D8Z9 (Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00720 PE=4 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.2e-292
Identity = 496/584 (84.93%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDG+ RCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWR+TL+RCTRLASALVR G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                        DS EQVAAL PNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDVC-----------------------DSFEQVAALVPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN RHDAAMVSTLL+HSEAK+IVVDYQ EHIV GAI  +SER+EKLP VVIIQE DQP
Sbjct: 121 CSLNTRHDAAMVSTLLSHSEAKIIVVDYQLEHIVTGAINTMSERKEKLPRVVIIQEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PSHI+  GSA   LEFES LASG+L+FEIRRP+DEWDPIALNYTSGTTSRPKGVIYSHRG
Sbjct: 181 PSHIDRPGSA---LEFESFLASGELDFEIRRPRDEWDPIALNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+LSAVLLNDMCSLPVYLWTVPMFHCNGWCLTW VAAQ GTNICQRNVTAKEIFDNI
Sbjct: 241 AYLNALSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTV NMIINAPISEQKPLP +VTMM+GGAPPPSHVLYK++++GF IVH+
Sbjct: 301 SLHKVTHMGGAPTVSNMIINAPISEQKPLPRQVTMMSGGAPPPSHVLYKLKALGFRIVHS 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGP TVCSWKPEWDSL  D QAKLNSRQGLQHIGLE VDIKDPVTM+S PADGK
Sbjct: 361 YGLTETYGPGTVCSWKPEWDSLPQDKQAKLNSRQGLQHIGLEAVDIKDPVTMKSVPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           TMGEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGV+HPDGYIELKDRSKDIIISGG
Sbjct: 421 TMGEVMLRGNTVMSGYLKDLKATQEAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENIS+IEVES+LFSHPSVL+AAVVGRPDDHWGETPCAF+KLKDGCSA+EEEII FC++HL
Sbjct: 481 ENISTIEVESVLFSHPSVLDAAVVGRPDDHWGETPCAFLKLKDGCSATEEEIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVF++LPKTSTGKTQKFILK+EAKAMGSLPKR+SKL
Sbjct: 541 PHYMAPRSVVFRELPKTSTGKTQKFILKKEAKAMGSLPKRVSKL 558

BLAST of Spg007229 vs. ExPASy TrEMBL
Match: A0A6J1BTA0 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111005519 PE=4 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.4e-291
Identity = 491/584 (84.08%), Postives = 529/584 (90.58%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDG DRCSAN+VPLTPITFLERSAAVYGDRISLVYGR++YTW++TLQRCTRLASALVR+G
Sbjct: 1   MDGADRCSANHVPLTPITFLERSAAVYGDRISLVYGRLRYTWKDTLQRCTRLASALVRLG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IA GDV                             VAALAPNIPAMYELHFAVPMAGAVL
Sbjct: 61  IAPGDV-----------------------------VAALAPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           CTLN+RHDAAMVSTLL HSEAK+I+VDYQF HIVKGAI+++S+R+EKLPLVVIIQE DQP
Sbjct: 121 CTLNIRHDAAMVSTLLTHSEAKIIIVDYQFLHIVKGAIEVMSQRKEKLPLVVIIQEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
            SH + AGSAS DLEFE LLA+G+L+FEIRRPKDEWDPI+LNYTSGTTSRPKGVIYSHRG
Sbjct: 181 SSHKDRAGSASEDLEFEKLLATGELDFEIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLNSLS VLLN MCS P+YLWTVPMFHCNGWC TWAVAAQGGTN+CQRNVTAKEIFDNI
Sbjct: 241 AYLNSLSTVLLNGMCSNPIYLWTVPMFHCNGWCFTWAVAAQGGTNVCQRNVTAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           S HKVTHM GAPTVLNMIINAPISEQKPLP KVT+MTGGAPPPSHVLYKMR++GFF+VH+
Sbjct: 301 SQHKVTHMAGAPTVLNMIINAPISEQKPLPEKVTVMTGGAPPPSHVLYKMRALGFFVVHS 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVC WKPEWDSL  D QAKLNSRQGLQH+G+E+VDIKDPVTMES PADGK
Sbjct: 361 YGLTETYGPATVCVWKPEWDSLPQDKQAKLNSRQGLQHVGMEEVDIKDPVTMESVPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           TMGEVMFRGNTVM+GYLKDLKAT+EAFNGGWFRSGDLGVRH DGYIELKDRSKDIIISGG
Sbjct: 421 TMGEVMFRGNTVMNGYLKDLKATREAFNGGWFRSGDLGVRHLDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES+LFSHP+VLEAAVVGRPDDHWGETPCAFVKLKDGCSA+EEEII FC++HL
Sbjct: 481 ENISSIEVESVLFSHPAVLEAAVVGRPDDHWGETPCAFVKLKDGCSATEEEIIEFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYM+PR VVFKDLPKTSTGKTQKFILK+EAKAMGSL KRISKL
Sbjct: 541 PHYMSPRCVVFKDLPKTSTGKTQKFILKQEAKAMGSLSKRISKL 555

BLAST of Spg007229 vs. ExPASy TrEMBL
Match: A0A0A0L1B6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658530 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 2.9e-289
Identity = 494/584 (84.59%), Postives = 523/584 (89.55%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           MDG  RCSANYVPLTPI+FLERSAAVYGDRISLVYGRVQYTWR+TLQRCTRLASALVR G
Sbjct: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           IARGDV                             VAAL PNIPAMYELHFAVPMAGAVL
Sbjct: 61  IARGDV-----------------------------VAALVPNIPAMYELHFAVPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C+LN RHDAAMVSTLL+HSEAK+IVVDYQ EHIV GAIK +SER+EKLP VVIIQE DQP
Sbjct: 121 CSLNTRHDAAMVSTLLSHSEAKIIVVDYQLEHIVTGAIKAMSERKEKLPRVVIIQEYDQP 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
           PS I+  GSA   LEFES LASGKLNFEIRRP+DE DPIALNYTSGTTSRPKGVI+SHRG
Sbjct: 181 PSRIDRPGSALEYLEFESFLASGKLNFEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTW VAAQ GTNICQRNVTAKEIFDNI
Sbjct: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           SLHKVTHMGGAPTV NMIINAPISEQKPLP +VTMM+GGAPPPSHVLYK++++GF IVH+
Sbjct: 301 SLHKVTHMGGAPTVSNMIINAPISEQKPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHS 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGPATVCSWKPEWDSL  D QAKLNSRQGLQHIGLE  D+KDPVTMES PADGK
Sbjct: 361 YGLTETYGPATVCSWKPEWDSLPQDKQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           TMGEVM RGNTVMSGYLKDLKAT+EAFNGGWFRSGDLGV+HPDGYIELKDRSKDIIISGG
Sbjct: 421 TMGEVMLRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENIS+IEVES+LFSHPSVL+AAVVGRPDDHWGETPCAFVKLKDGCSA+E EII FC++HL
Sbjct: 481 ENISTIEVESVLFSHPSVLDAAVVGRPDDHWGETPCAFVKLKDGCSATEGEIIKFCREHL 540

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKRISKL 585
           PHYMAPRSVVF+DLPKTSTGKTQKFILK+EAKAMGSLPKR+SKL
Sbjct: 541 PHYMAPRSVVFRDLPKTSTGKTQKFILKKEAKAMGSLPKRVSKL 555

BLAST of Spg007229 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 785.0 bits (2026), Expect = 4.1e-227
Identity = 374/580 (64.48%), Postives = 457/580 (78.79%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           M+G  +  ANYVPLTPI+FL+RSA VY DR+S+VYG V+YTWR+T  RC R+ASAL ++G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           I+ GDV                             V+ LAPN+PAM ELHF VPMAGA+L
Sbjct: 63  ISTGDV-----------------------------VSVLAPNVPAMVELHFGVPMAGALL 122

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           CTLN+RHD+++V+ LL HS  K+I  D+QF  I +GA +ILS + +K+P++V+I E    
Sbjct: 123 CTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKGDKVPILVLIPE--PL 182

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
              ++    +   +E+E ++A GK +FE+ RP DE D I++NYTSGTTS PKGV+YSHRG
Sbjct: 183 TQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGTTSSPKGVVYSHRG 242

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLNSL+AVLLN+M S P YLWT PMFHCNGWCL W V A GGTNIC RNVTAK IFDNI
Sbjct: 243 AYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNI 302

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
           S HKVTHMGGAPT+LNMIINAP SEQKPLPGKV+ +TG APPP+HV++KM  +GF + H+
Sbjct: 303 SQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHVIFKMEELGFSMFHS 362

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YGLTETYGP T+C+WKPEWDSL  + QAK+ +RQG+ H+GLE++ +KDPVTM + PADG 
Sbjct: 363 YGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGV 422

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           TMGEV+FRGNTVM+GYLK+ +ATKEAF GGWF SGDLGV+HPDGYIELKDRSKDIIISGG
Sbjct: 423 TMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYIELKDRSKDIIISGG 482

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           ENISSIEVES LF+HP VLEAAVV RPD++WGET CAFVKLKDG  AS EE+I++C+  L
Sbjct: 483 ENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRL 542

Query: 541 PHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKR 581
           PHYMAPRS+VF+DLPKTSTGK QKF+L+ +AKA+ SL K+
Sbjct: 543 PHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLSKK 551

BLAST of Spg007229 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 731.1 bits (1886), Expect = 7.0e-211
Identity = 357/585 (61.03%), Postives = 438/585 (74.87%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           ++G+ R  AN+ PL+PITFLERSA VY DR SLV+G V++TW +T QRC RLASAL  +G
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           I+RGDV                             VAALAPN+PAM+ELHFAVPMAG +L
Sbjct: 108 ISRGDV-----------------------------VAALAPNVPAMHELHFAVPMAGLIL 167

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILS---ERREKLPLVVIIQES 180
           C LN R D + +S LL HSEAK++ VD+Q   I  GA+ +L+   + R+ L LV+I Q +
Sbjct: 168 CPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLAKSDKTRKSLKLVLISQSN 227

Query: 181 DQPPSHING----AGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKG 240
           D   S  +     A   S D E+E+LL SG   FEI +P+ EWDPI++NYTSGTTSRPKG
Sbjct: 228 DDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCEWDPISINYTSGTTSRPKG 287

Query: 241 VIYSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTA 300
           V+YSHRGAYLNSL+ V L+ M   PVYLWTVPMFHCNGWCL W VAAQGGTNIC R V+ 
Sbjct: 288 VVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSP 347

Query: 301 KEIFDNISLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSM 360
           K IF NI++HKVTHMGGAPTVLNMI+N  ++E KPLP +V +MTGG+PP   +L KM  +
Sbjct: 348 KMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEEL 407

Query: 361 GFFIVHAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTME 420
           GF + H YGLTETYGP T C WKPEWDSLS + + KL +RQG+QH+GLE +D+KDP+TME
Sbjct: 408 GFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTME 467

Query: 421 SAPADGKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSK 480
           + P DG TMGEVMFRGNTVMSGY KD++AT++AF G WF SGDL V++PDGYIE+KDR K
Sbjct: 468 TVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLK 527

Query: 481 DIIISGGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGC-SASEEEI 540
           D+IISGGENISS+EVE +L SH +VLEAAVV RPD HWG+TPC FVKLK+G  +   EEI
Sbjct: 528 DVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEI 587

Query: 541 INFCKKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSL 578
           I FC+ HLPHYMAP+++VF D+PKTSTGK QK++L+++A  MGSL
Sbjct: 588 IGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEMGSL 603

BLAST of Spg007229 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 694.5 bits (1791), Expect = 7.3e-200
Identity = 325/475 (68.42%), Postives = 394/475 (82.95%), Query Frame = 0

Query: 106 MYELHFAVPMAGAVLCTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERR 165
           M ELHF VPMAGA+LCTLN+RHD+++V+ LL HS  K+I  D+QF  I +GA +ILS + 
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 166 EKLPLVVIIQESDQPPSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTS 225
           +K+P++V+I E       ++    +   +E+E ++A GK +FE+ RP DE D I++NYTS
Sbjct: 61  DKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTS 120

Query: 226 GTTSRPKGVIYSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTN 285
           GTTS PKGV+YSHRGAYLNSL+AVLLN+M S P YLWT PMFHCNGWCL W V A GGTN
Sbjct: 121 GTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTN 180

Query: 286 ICQRNVTAKEIFDNISLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSH 345
           IC RNVTAK IFDNIS HKVTHMGGAPT+LNMIINAP SEQKPLPGKV+ +TG APPP+H
Sbjct: 181 ICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAH 240

Query: 346 VLYKMRSMGFFIVHAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVD 405
           V++KM  +GF + H+YGLTETYGP T+C+WKPEWDSL  + QAK+ +RQG+ H+GLE++ 
Sbjct: 241 VIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQ 300

Query: 406 IKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGY 465
           +KDPVTM + PADG TMGEV+FRGNTVM+GYLK+ +ATKEAF GGWF SGDLGV+HPDGY
Sbjct: 301 VKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGY 360

Query: 466 IELKDRSKDIIISGGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGC 525
           IELKDRSKDIIISGGENISSIEVES LF+HP VLEAAVV RPD++WGET CAFVKLKDG 
Sbjct: 361 IELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGS 420

Query: 526 SASEEEIINFCKKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKR 581
            AS EE+I++C+  LPHYMAPRS+VF+DLPKTSTGK QKF+L+ +AKA+ SL K+
Sbjct: 421 KASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLSKK 473

BLAST of Spg007229 vs. TAIR 10
Match: AT3G16910.1 (acyl-activating enzyme 7 )

HSP 1 Score: 592.8 bits (1527), Expect = 3.0e-169
Identity = 294/588 (50.00%), Postives = 394/588 (67.01%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           +D + +  ANY  LTP+ FL+R+A V+  R S+++G  +YTWR+T  RC RLASAL    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           I  G                               VA +APNIPAMYE HF VPM GAVL
Sbjct: 69  IGPGST-----------------------------VAIIAPNIPAMYEAHFGVPMCGAVL 128

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERRE---KLPLVVIIQES 180
             +N+R +A  V+ LL+HS++ +I+VD +F  + + +++++ E+     K PL+++I + 
Sbjct: 129 NCVNIRLNAPTVAFLLSHSQSSVIMVDQEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDH 188

Query: 181 DQPPSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYS 240
              P  +N A S  G +E+E  LA+G  N+  + P DEW  IAL YTSGTT+ PKGV+  
Sbjct: 189 TCAPESLNRALS-KGAIEYEDFLATGDPNYPWQPPADEWQSIALGYTSGTTASPKGVVLH 248

Query: 241 HRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIF 300
           HRGAY+ +LS  L+  M    VYLWT+PMFHCNGWC  W++A   GT+IC R VTAKE++
Sbjct: 249 HRGAYIMALSNPLIWGMQDGAVYLWTLPMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVY 308

Query: 301 DNISLHKVTHMGGAPTVLNMIINAPISEQ-KPLPGKVTMMTGGAPPPSHVLYKMRSMGFF 360
             I+ +KVTH   AP VLN I+NAP  +   PLP  V +MT GA PP  VL+ M   GF 
Sbjct: 309 SMIAKYKVTHFCAAPVVLNAIVNAPKEDTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFR 368

Query: 361 IVHAYGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAP 420
           + H YGL+ETYGP+TVC+WKPEWDSL  + QAKLN+RQG+++ G+E +D+ D  T +  P
Sbjct: 369 VAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNARQGVRYTGMEQLDVIDTQTGKPVP 428

Query: 421 ADGKTMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDII 480
           ADGKT GE++FRGN VM GYLK+ +A KE F GGWF SGD+ V+HPD YIE+KDRSKD+I
Sbjct: 429 ADGKTAGEIVFRGNMVMKGYLKNPEANKETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVI 488

Query: 481 ISGGENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASE-----EE 540
           ISGGENISS+EVE++++ HP+VLEA+VV RPD+ W E+PCAFV LK      +     ++
Sbjct: 489 ISGGENISSVEVENVVYHHPAVLEASVVARPDERWQESPCAFVTLKSDYEKHDQNKLAQD 548

Query: 541 IINFCKKHLPHYMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPK 580
           I+ FC++ LP Y  P+SVVF  LPKT+TGK QK IL+ +AK MG +P+
Sbjct: 549 IMKFCREKLPAYWVPKSVVFGPLPKTATGKIQKHILRTKAKEMGPVPR 566

BLAST of Spg007229 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 568.2 bits (1463), Expect = 7.9e-162
Identity = 288/583 (49.40%), Postives = 383/583 (65.69%), Query Frame = 0

Query: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLQRCTRLASALVRMG 60
           M+G  RCSANYVPL+PI+FLER+A V+G R S+VYG +QYTW +T  RC RLASAL  +G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  IARGDVSVRYLVRLSSFLITISRSNFAAEYDSLEQVAALAPNIPAMYELHFAVPMAGAVL 120
           ++R DV                             VAALAPN+PA+ EL+F  PMAGAVL
Sbjct: 61  LSRHDV-----------------------------VAALAPNVPALCELYFGAPMAGAVL 120

Query: 121 CTLNMRHDAAMVSTLLNHSEAKMIVVDYQFEHIVKGAIKILSERREKLPLVVIIQESDQP 180
           C LN   D+ M++  L  ++ K+  VD +F  + + ++ +LS   EK PL++ I E+   
Sbjct: 121 CVLNTTFDSQMLAMALEKTKPKVFFVDSEFLSVAEESLSLLSNIEEK-PLIITITENPTE 180

Query: 181 PSHINGAGSASGDLEFESLLASGKLNFEIRRPKDEWDPIALNYTSGTTSRPKGVIYSHRG 240
            S            ++E  L++G  NF+  RP DE DPIALN+TSGTTS PK V+YSHRG
Sbjct: 181 QSKYE---------QYEDFLSTGNPNFKPIRPVDECDPIALNFTSGTTSTPKCVVYSHRG 240

Query: 241 AYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGWCLTWAVAAQGGTNICQRNVTAKEIFDNI 300
           AYLN+ +  ++N+M  +PVYL TVPM+HC+GWC  W V A GG  +C R V  + IFD+I
Sbjct: 241 AYLNATAVGVMNEMKPMPVYLCTVPMYHCSGWCYIWTVTAFGGVIVCLREVNDEVIFDSI 300

Query: 301 SLHKVTHMGGAPTVLNMIINAPISEQKPLPGKVTMMTGGAPPPSHVLYKMRSMGFFIVHA 360
             HKVT+ GG+P VLNMI NA  S +K  P  V +M+GG+ PP  V+ K++ +GF ++ A
Sbjct: 301 VKHKVTNFGGSPPVLNMIANARDSVKKSFPWTVQVMSGGSSPP-EVMLKLKKLGFKVMMA 360

Query: 361 YGLTETYGPATVCSWKPEWDSLSNDMQAKLNSRQGLQHIGLEDVDIKDPVTMESAPADGK 420
           YG +E YG  T C W PEW++L  +   +L +R GL H   E VD+ DP TM+S P DGK
Sbjct: 361 YGCSEVYGLGTACLWMPEWETLPEEESLRLKARDGLNHFAKEAVDVLDPTTMKSVPHDGK 420

Query: 421 TMGEVMFRGNTVMSGYLKDLKATKEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGG 480
           T+  +  RGNTVMSGY KD +AT+ AF GGW+ S D+GV  PDGYI+ KDRS+D+I  GG
Sbjct: 421 TIRVIALRGNTVMSGYFKDKEATEAAFRGGWYWSRDMGVIDPDGYIQFKDRSQDVITCGG 480

Query: 481 ENISSIEVESMLFSHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSASEEEIINFCKKHL 540
           E + S E+E +L+SHP+V +A VVGRPD+  GE+ CAFVKLK+G  A EEEII FCK+ L
Sbjct: 481 EIVGSKEIEGILYSHPAVYDAGVVGRPDETLGESMCAFVKLKEGAEAREEEIIEFCKRKL 540

Query: 541 PH---YMAPRSVVFKDLPKTSTGKTQKFILKEEAKAMGSLPKR 581
            +    M P++VVF D+PKT TGK +K +L++ AK MG +  R
Sbjct: 541 GNKNMKMIPKTVVFSDVPKTPTGKIRKNVLRKMAKDMGYVQLR 543