Spg006800 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg006800
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DNA-directed DNA polymerase
Location	scaffold1: 55301188 .. 55304505 (-)
RNA-Seq Expression	Spg006800
Synteny	Spg006800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGACGTCCGTCTTGGAGGAGGACCACCCAAGGTGAAATTTGATGCATTTATCTCCACTTTTAAAAATTTTGGATCGACAATTCCATATACTGGTCCTCAAAAGCCCTCAAAGATCGCCTTGACATCAGAAGCTTTGACGATCAAATGAATCCGACATTTGGCAGTCCATCAAGTTGTTTATGAGTTCAGCTAACTCAGGAAGGGCCATGTGATATGCATTCTTTCTGGTGGTTTCACCCCTGTCAACAATTCAATCTCTTGATCTATCCTTCTATGTGGAGGTATAGTCTTTGGCAGGCTATCAAGCATGTCAGCTCGGTACTCTTCCTACAATCAGTAGATCTCTTTGGTGATCAATTCTTTAGAACTCTCATCTTTGATCGCAAGAATGGTCATGAATGTTGGTTTTTCATGAGTGAGATTCCTATGTTTTCTCGAATCAATATGAGTCAGGTCTTTAGAGAGGATTCAATATCTAATGGTCTTCAATCATTCTGTGTTTCAGATATGCCCATCAAAGCCAAATTATTATGGTTTAATGCTACCAAGGCCTTTCTTTCATATATTTAGTTGGAAAGAAATTAAAGATTTTTTTAAGACAAATCTACAGTTTGGTTGGATTGTTTTGAGCTCATCAATATTGATGCTTGTTGATGGTGTTTTATGTCTAAATTCTTTGATGGTTATTCTTCTAAACTCTATTGACTTTGTTGTAACAATTGGAGGGCTTTCTTGTAGGCTTTTCAGATTCTTAGGCTTTTTTGTTTTTTGTATTTCTTTTCTGCATTTTACATTTATGCTTCAGTTTTATTTTCTTAGTCTGCACTTTTGTAGTATTTGAATTCTTGTATGCTCTTTTAGCTTTAGAAGGGTTTGTTAGTTTTCAGGCAAGAAATTCCATCTAAGTTCATTCTGTTTCTTGTTCAAGAAGCAACAAATTTAATGGCAAATATATATGTTTTAATTATTTAAGAATGCATCTTTACATTTAAATCTACTTGTATTAATAATCTATTGTTGATTTAATTCAAACTAAGTATCAACAAAATTGTACTATCTTCTGTATCTACATAGGATCCACTTTTCGTCATACACGTTACCCTGCATACAAGAGTAACAGGCCACCTACACCTGATACCATAGTCCAGGGGCTCCAATACTTGAAGGCATCCATAAAGTCTATGTCCGTAAAGGTGATTGAGGTAATAAAAAAATTATTTTTTCTTAGGTTTTTATTAGACTGCTTCCAGGTTTTCCCCTTATTTTTATTTTACAGATTTCCCCTGCTTTGCTTTTGCATTTAGGGACGTAAGATTTACATTTAGCTTTTTTTCTAGCAGCTCCTTAATGAAATTTATTTATTTTTTTTCTACAATTTGTTCAATCAGTTACCTGTACTAATCATCTTCTGGAATTAGTCATGCTTTTTGTTTATTTTGATTATTTAGCCTATTATTCTCTGATGGTTGACTTTTATTAGAGTCAATGATAGGTACCTGGAGTGGAAGCTGATGATGTAATTGGCACATTGGCTTTGAGAAGTGTTGCTGCTGGCTGTAAGGTGATATTTCAGAGGCATAGGGGCCCTTTTGAAATTTATTTTACATGCTTGCCAAAATTGTGCTCTGTTATGGGGGGATATATTTAGTCATCCTCTATTTGATCTTGTTGAGTGTCAAAACAGGTTCGCGTTGTCTCCCCTGACAAAGACTTCTTCCAGATTCTATCTCCTTCACTACGTCTTTTACGAATAGCTCCTCGTGGGTTTGAGTAAGAACTTATGGATTACTTGCTTATTCATTAAAAATGAACTCCTCTAACTTTATAAAAGAAATAGTTTTTCCCTAATAAATTTGTAGTTATTTTCTCTATTGACTCTGATGGTAGAATAGGTATATGTGGTACTTTTATGTCATTAATATTGCTCTCCTTATCGTTTCGGAGGGATTGGCATATGATTATTGTCCAAAACAGAACACCTGAAAATAATTTTTTCTCTCCGTGTTAAAGGACAACAACTACATATGCATGAAATGATATCTGGGTTTTTTTCAGTCAATATTTAGAATAGAAAACTAAAAAATCTCTGGTCTTTTTCCTTTCTTCTAATAGTTACTGGGAAAATGATAGATAATCAAAATCCCTTCTCTTTGAAAAATTTATTATTGAACCTGCATAGGATCCGTAGAATTTTTTCTAGAGGGAGTGGGACGTAGGAGGGTTCCATAAGAGTCGGGGAAGGAAATCCCCTCATTGTGAGTGAACAAGAGCAGTTTGTTTTTTGAACTAGTGACTTAGGAAAGAAGTTGGAAATGGAGGAAATTTGCAAAGGAAAGACATTAAAGATTTCATTGAACATTAGGTTGTATTTGGGTTTAGTTACTTGAGTAAGGATCAAAATATTGAGCTGATAAATGATCAAGACACTATCAGCTGGAATTTAGAAGGGTCTGGAAGTTTTTCGGCAAAATCTTGTTTCCTATCAATGACCTCTATATCTCCAAAGCTAAATCAGCCTAGTAGCAGTCTTATTTGGAAACATAAGAGCCCAAAGAAGGTGAAAGTGCTTCTTTGGTCTCTTATATACAGAAGTTTGAATACGGATGAGATGCTGCAAAGAAAGTTTGGTGGCTGGATGCTCTCTCCTTCGGCCTGCAGGTTGTGCATTAAGGCAACAGAAACCTTGGATCATTTATTCCTTCATTGTGAGTTTGCAGGGGCTGCTTGGAATTTTGTGGCCAATTTGCTGGGCATTTCGTTTTGCAAGCCAAAGAAGATTGAGGATTGGTTGTTAGAAGGCCTTAATGCTTGGAATCTAACAAGAAAGGCTAAGATCTTGGCTAGTTGTGTGTTTCGGGCCACCATTTGGAATTTGTGGAAGGAAAGAAATGCTAGAACATTTGAGGACAAGTGTGGTAGCTTTGCTTCTTTTTGTGATAATGTACAAAATATAGCTGCTTGGTGGATATCTTTACATAAGAATTCTTTTTGTAATTACAGCCTGCCAATGATTATTAGTGATTGGAAGGCTTTGTTAAAGTAGTTTCCGGGCTTGGGCTCCCTCCACCCTTGTCCCTTAGGCTGTTCCCACCTTTTTTTGTGATTAATATATCTCTCTTTGATGTTTCTTGTCAAAAAAAAAAAAAAAAAAGTTACTTGAGTAAGGATCTATTTTCATGTTGTTGGGTCATGTTCGAAATATTGATCTAGATTCAGATGGTTTGAAACCCTGGAAGAATTGATGTTGTAGGGGTGGAGCCTCCCAAGTGGAGGTCTTCGATTATCATCACTGATGGAGAAGAAAGATCTGGGTGA

mRNA sequence

ATGGGACGTCCGTCTTGGAGGAGGACCACCCAAGGATCCACTTTTCGTCATACACGTTACCCTGCATACAAGAGTAACAGGCCACCTACACCTGATACCATAGTCCAGGGGCTCCAATACTTGAAGGCATCCATAAAGTCTATGTCCGTAAAGGTGATTGAGGTACCTGGAGTGGAAGCTGATGATGTAATTGGCACATTGGCTTTGAGAAGTGTTGCTGCTGGCTGTAAGGTTCGCGTTGTCTCCCCTGACAAAGACTTCTTCCAGATTCTATCTCCTTCACTACGTCTTTTACGAATAGCTCCTCGTGGGTTTGAAAGTTTGAATACGGATGAGATGCTGCAAAGAAAGTTTGGTGGCTGGATGCTCTCTCCTTCGGCCTGCAGGTTGTGCATTAAGGCAACAGAAACCTTGGATCATTTATTCCTTCATTGTGAGTTTGCAGGGGCTGCTTGGAATTTTGTGGCCAATTTGCTGGGCATTTCGTTTTGCAAGCCAAAGAAGATTGAGGATTGGTTGTTAGAAGGCCTTAATGCTTGGAATCTAACAAGAAAGGCTAAGATCTTGGCTAGTTGTGTGTTTCGGGCCACCATTTGGAATTTGTGGAAGGAAAGAAATGCTAGAACATTTGAGGACAAGTGTGGGGTGGAGCCTCCCAAGTGGAGGTCTTCGATTATCATCACTGATGGAGAAGAAAGATCTGGGTGA

Coding sequence (CDS)

ATGGGACGTCCGTCTTGGAGGAGGACCACCCAAGGATCCACTTTTCGTCATACACGTTACCCTGCATACAAGAGTAACAGGCCACCTACACCTGATACCATAGTCCAGGGGCTCCAATACTTGAAGGCATCCATAAAGTCTATGTCCGTAAAGGTGATTGAGGTACCTGGAGTGGAAGCTGATGATGTAATTGGCACATTGGCTTTGAGAAGTGTTGCTGCTGGCTGTAAGGTTCGCGTTGTCTCCCCTGACAAAGACTTCTTCCAGATTCTATCTCCTTCACTACGTCTTTTACGAATAGCTCCTCGTGGGTTTGAAAGTTTGAATACGGATGAGATGCTGCAAAGAAAGTTTGGTGGCTGGATGCTCTCTCCTTCGGCCTGCAGGTTGTGCATTAAGGCAACAGAAACCTTGGATCATTTATTCCTTCATTGTGAGTTTGCAGGGGCTGCTTGGAATTTTGTGGCCAATTTGCTGGGCATTTCGTTTTGCAAGCCAAAGAAGATTGAGGATTGGTTGTTAGAAGGCCTTAATGCTTGGAATCTAACAAGAAAGGCTAAGATCTTGGCTAGTTGTGTGTTTCGGGCCACCATTTGGAATTTGTGGAAGGAAAGAAATGCTAGAACATTTGAGGACAAGTGTGGGGTGGAGCCTCCCAAGTGGAGGTCTTCGATTATCATCACTGATGGAGAAGAAAGATCTGGGTGA

Protein sequence

MGRPSWRRTTQGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRSVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTDEMLQRKFGGWMLSPSACRLCIKATETLDHLFLHCEFAGAAWNFVANLLGISFCKPKKIEDWLLEGLNAWNLTRKAKILASCVFRATIWNLWKERNARTFEDKCGVEPPKWRSSIIITDGEERSG

Homology

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BLAST of Spg006800 vs. NCBI nr
Match: XP_038899891.1 (DNA polymerase I isoform X1 [Benincasa hispida])

HSP 1 Score: 191.4 bits (485), Expect = 8.6e-45
Identity = 100/117 (85.47%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMS+KVIEVPGVEADDVIGTLALR
Sbjct: 206 KGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSIKVIEVPGVEADDVIGTLALR 265

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 266 SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFEMVSFGLEEFAEKYG--VLQPS 320

[Close]

BLAST of Spg006800 vs. NCBI nr
Match: XP_038899894.1 (DNA polymerase I, thermostable isoform X3 [Benincasa hispida])

HSP 1 Score: 191.4 bits (485), Expect = 8.6e-45
Identity = 100/117 (85.47%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMS+KVIEVPGVEADDVIGTLALR
Sbjct: 193 KGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSIKVIEVPGVEADDVIGTLALR 252

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 253 SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFEMVSFGLEEFAEKYG--VLQPS 307

[Close]

BLAST of Spg006800 vs. NCBI nr
Match: XP_038899895.1 (DNA polymerase I isoform X4 [Benincasa hispida])

HSP 1 Score: 191.0 bits (484), Expect = 1.1e-44
Identity = 100/116 (86.21%), Postives = 105/116 (90.52%), Query Frame = 0


Query: 12  GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 71
           GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMS+KVIEVPGVEADDVIGTLALRS
Sbjct: 189 GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSIKVIEVPGVEADDVIGTLALRS 248

Query: 72  VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 249 VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFEMVSFGLEEFAEKYG--VLQPS 302

[Close]

BLAST of Spg006800 vs. NCBI nr
Match: XP_038899897.1 (DNA polymerase I isoform X6 [Benincasa hispida])

HSP 1 Score: 191.0 bits (484), Expect = 1.1e-44
Identity = 100/116 (86.21%), Postives = 105/116 (90.52%), Query Frame = 0


Query: 12  GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 71
           GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMS+KVIEVPGVEADDVIGTLALRS
Sbjct: 176 GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSIKVIEVPGVEADDVIGTLALRS 235

Query: 72  VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 236 VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFEMVSFGLEEFAEKYG--VLQPS 289

[Close]

BLAST of Spg006800 vs. NCBI nr
Match: XP_022933106.1 (uncharacterized protein LOC111439872 isoform X1 [Cucurbita moschata])

HSP 1 Score: 190.7 bits (483), Expect = 1.5e-44
Identity = 100/117 (85.47%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR
Sbjct: 203 KGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 262

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           SVAAGCKVRVVSPDKDFFQI+SPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 263 SVAAGCKVRVVSPDKDFFQIISPSLRLLRIAPRGFEMVSFGLEDFAEKYG--VLEPS 317

[Close]

BLAST of Spg006800 vs. ExPASy Swiss-Prot
Match: O52225 (DNA polymerase I, thermostable OS=Thermus filiformis OX=276 GN=polA PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.2e-14
Identity = 43/106 (40.57%), Postives = 67/106 (63.21%), Query Frame = 0


Query: 14  TFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRSVA 73
           +FRH  Y AYK+ R PTP+   + L  +K  +  + +  +E PG EADDV+GTLA ++  
Sbjct: 71  SFRHEAYEAYKAGRAPTPEDFPRQLALVKRLVDLLGLVRLEAPGYEADDVLGTLAKKAER 130

Query: 74  AGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTDEMLQRKFG 120
            G +VR+++ D+DFFQ+LS  + +L   P G  +L T + +Q K+G
Sbjct: 131 EGMEVRILTGDRDFFQLLSEKVSVL--LPDG--TLVTPKDVQEKYG 172

[Close]

BLAST of Spg006800 vs. ExPASy Swiss-Prot
Match: P30313 (DNA polymerase I, thermostable OS=Thermus thermophilus OX=274 GN=polA PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 8.2e-14
Identity = 42/106 (39.62%), Postives = 64/106 (60.38%), Query Frame = 0


Query: 14  TFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRSVA 73
           +FRH  Y AYK+ R PTP+   + L  +K  +  + +  +EVPG EADDV+ TLA R+  
Sbjct: 71  SFRHEAYEAYKAGRAPTPEDFPRQLALIKELVDLLGLVRLEVPGFEADDVLATLAKRAEK 130

Query: 74  AGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTDEMLQRKFG 120
            G +VR+++ D+D +Q+LS  + +L   P G+  L T   L  K+G
Sbjct: 131 EGYEVRILTADRDLYQLLSERIAILH--PEGY--LITPAWLYEKYG 172

[Close]

BLAST of Spg006800 vs. ExPASy Swiss-Prot
Match: Q55971 (DNA polymerase I OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=polA PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.1e-13
Identity = 35/80 (43.75%), Postives = 55/80 (68.75%), Query Frame = 0


Query: 14  TFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRSVA 73
           TFRH    AYKSNR  TP+   + L YL+  +++++++ I   G EADD++GTLA +   
Sbjct: 74  TFRHEADGAYKSNRQETPEDFAEDLSYLQQLLEALNLQTITYAGYEADDILGTLACQGSD 133

Query: 74  AGCKVRVVSPDKDFFQILSP 94
           AG +V+++S D+D FQ++SP
Sbjct: 134 AGYQVKILSGDRDLFQLVSP 153

[Close]

BLAST of Spg006800 vs. ExPASy Swiss-Prot
Match: P52028 (DNA polymerase I, thermostable OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=polA PE=3 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 5.3e-13
Identity = 41/106 (38.68%), Postives = 63/106 (59.43%), Query Frame = 0


Query: 14  TFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRSVA 73
           +FRH  Y AYK+ R PTP+   + L  +K  +  +    +EVPG EADDV+ TLA ++  
Sbjct: 73  SFRHEAYEAYKAGRAPTPEDFPRQLALIKELVDLLGFTRLEVPGYEADDVLATLAKKAEK 132

Query: 74  AGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTDEMLQRKFG 120
            G +VR+++ D+D +Q++S  + +L   P G   L T E L  K+G
Sbjct: 133 EGYEVRILTADRDLYQLVSDRVAVLH--PEG--HLITPEWLWEKYG 174

[Close]

BLAST of Spg006800 vs. ExPASy Swiss-Prot
Match: P00582 (DNA polymerase I OS=Escherichia coli (strain K12) OX=83333 GN=polA PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.3e-13
Identity = 38/88 (43.18%), Postives = 58/88 (65.91%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +G TFR   +  YKS+RPP PD +   ++ L A +K+M + ++ V GVEADDVIGTLA  
Sbjct: 65  KGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAMGLPLLAVSGVEADDVIGTLARE 124

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLL 99
           +  AG  V + + DKD  Q+++P++ L+
Sbjct: 125 AEKAGRPVLISTGDKDMAQLVTPNITLI 152

[Close]

BLAST of Spg006800 vs. ExPASy TrEMBL
Match: A0A6J1HVF9 (DNA-directed DNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111467206 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 7.1e-45
Identity = 100/117 (85.47%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR
Sbjct: 203 KGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 262

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           SVAAGCKVRVVSPDKDFFQI+SPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 263 SVAAGCKVRVVSPDKDFFQIISPSLRLLRIAPRGFEMVSFGLEDFAEKYG--VLEPS 317

[Close]

BLAST of Spg006800 vs. ExPASy TrEMBL
Match: A0A6J1EY78 (DNA-directed DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111439872 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 7.1e-45
Identity = 100/117 (85.47%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR
Sbjct: 203 KGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 262

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           SVAAGCKVRVVSPDKDFFQI+SPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 263 SVAAGCKVRVVSPDKDFFQIISPSLRLLRIAPRGFEMVSFGLEDFAEKYG--VLEPS 317

[Close]

BLAST of Spg006800 vs. ExPASy TrEMBL
Match: A0A6J1F3S3 (DNA-directed DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111439872 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 9.3e-45
Identity = 100/116 (86.21%), Postives = 105/116 (90.52%), Query Frame = 0


Query: 12  GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 71
           GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS
Sbjct: 186 GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 245

Query: 72  VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           VAAGCKVRVVSPDKDFFQI+SPSLRLLRIAPRGFE ++   E    K+G  +L PS
Sbjct: 246 VAAGCKVRVVSPDKDFFQIISPSLRLLRIAPRGFEMVSFGLEDFAEKYG--VLEPS 299

[Close]

BLAST of Spg006800 vs. ExPASy TrEMBL
Match: A0A6J1D5H7 (DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 5.1e-43
Identity = 98/117 (83.76%), Postives = 102/117 (87.18%), Query Frame = 0


Query: 11  QGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 70
           +GSTFRH  YPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR
Sbjct: 155 KGSTFRHNIYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALR 214

Query: 71  SVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           SVA GCKVRVVSPDKDFFQILSPSLRLLRIAPRGFE ++   E    K+G   L PS
Sbjct: 215 SVATGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFEMVSFGLEDFAEKYGA--LEPS 269

[Close]

BLAST of Spg006800 vs. ExPASy TrEMBL
Match: A0A6J1D7Q6 (DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 5.1e-43
Identity = 98/116 (84.48%), Postives = 101/116 (87.07%), Query Frame = 0


Query: 12  GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 71
           GSTFRH  YPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS
Sbjct: 187 GSTFRHNIYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 246

Query: 72  VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFGGWMLSPS 127
           VA GCKVRVVSPDKDFFQILSPSLRLLRIAPRGFE ++   E    K+G   L PS
Sbjct: 247 VATGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFEMVSFGLEDFAEKYGA--LEPS 300

[Close]

BLAST of Spg006800 vs. TAIR 10
Match: AT3G52050.2 (5'-3' exonuclease family protein )

HSP 1 Score: 173.3 bits (438), Expect = 2.3e-43
Identity = 88/113 (77.88%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 8   RTTQGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTL 67
           R+ +G  FRHT YPAYKSNRPPTPDTIVQGLQYLKASIK+MS+KVIEVPGVEADDVIGTL
Sbjct: 174 RSAKGMNFRHTLYPAYKSNRPPTPDTIVQGLQYLKASIKAMSIKVIEVPGVEADDVIGTL 233

Query: 68  ALRSVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFG 120
           A+RS++AG KVRVVSPDKDFFQILSPSLRLLR+ PRG E  +   E   +KFG
Sbjct: 234 AMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMEDFAKKFG 286

[Close]

BLAST of Spg006800 vs. TAIR 10
Match: AT3G52050.1 (5'-3' exonuclease family protein )

HSP 1 Score: 173.3 bits (438), Expect = 2.3e-43
Identity = 88/113 (77.88%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 8   RTTQGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTL 67
           R+ +G  FRHT YPAYKSNRPPTPDTIVQGLQYLKASIK+MS+KVIEVPGVEADDVIGTL
Sbjct: 174 RSAKGMNFRHTLYPAYKSNRPPTPDTIVQGLQYLKASIKAMSIKVIEVPGVEADDVIGTL 233

Query: 68  ALRSVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFG 120
           A+RS++AG KVRVVSPDKDFFQILSPSLRLLR+ PRG E  +   E   +KFG
Sbjct: 234 AMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMEDFAKKFG 286

[Close]

BLAST of Spg006800 vs. TAIR 10
Match: AT3G52050.3 (5'-3' exonuclease family protein )

HSP 1 Score: 173.3 bits (438), Expect = 2.3e-43
Identity = 88/113 (77.88%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 8   RTTQGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTL 67
           R+ +G  FRHT YPAYKSNRPPTPDTIVQGLQYLKASIK+MS+KVIEVPGVEADDVIGTL
Sbjct: 197 RSAKGMNFRHTLYPAYKSNRPPTPDTIVQGLQYLKASIKAMSIKVIEVPGVEADDVIGTL 256

Query: 68  ALRSVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFG 120
           A+RS++AG KVRVVSPDKDFFQILSPSLRLLR+ PRG E  +   E   +KFG
Sbjct: 257 AMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMEDFAKKFG 309

[Close]

BLAST of Spg006800 vs. TAIR 10
Match: AT3G52050.4 (5'-3' exonuclease family protein )

HSP 1 Score: 173.3 bits (438), Expect = 2.3e-43
Identity = 88/113 (77.88%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 8   RTTQGSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTL 67
           R+ +G  FRHT YPAYKSNRPPTPDTIVQGLQYLKASIK+MS+KVIEVPGVEADDVIGTL
Sbjct: 162 RSAKGMNFRHTLYPAYKSNRPPTPDTIVQGLQYLKASIKAMSIKVIEVPGVEADDVIGTL 221

Query: 68  ALRSVAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFG 120
           A+RS++AG KVRVVSPDKDFFQILSPSLRLLR+ PRG E  +   E   +KFG
Sbjct: 222 AMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMEDFAKKFG 274

[Close]

BLAST of Spg006800 vs. TAIR 10
Match: AT3G52050.5 (5'-3' exonuclease family protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.1e-42
Identity = 87/109 (79.82%), Postives = 95/109 (87.16%), Query Frame = 0


Query: 12  GSTFRHTRYPAYKSNRPPTPDTIVQGLQYLKASIKSMSVKVIEVPGVEADDVIGTLALRS 71
           G  FRHT YPAYKSNRPPTPDTIVQGLQYLKASIK+MS+KVIEVPGVEADDVIGTLA+RS
Sbjct: 183 GMNFRHTLYPAYKSNRPPTPDTIVQGLQYLKASIKAMSIKVIEVPGVEADDVIGTLAMRS 242

Query: 72  VAAGCKVRVVSPDKDFFQILSPSLRLLRIAPRGFESLNTD-EMLQRKFG 120
           ++AG KVRVVSPDKDFFQILSPSLRLLR+ PRG E  +   E   +KFG
Sbjct: 243 ISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMEDFAKKFG 291

The following BLAST results are available for this feature:

BLAST of Spg006800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899891.1	8.6e-45	85.47	DNA polymerase I isoform X1 [Benincasa hispida]	[more]
XP_038899894.1	8.6e-45	85.47	DNA polymerase I, thermostable isoform X3 [Benincasa hispida]	[more]
XP_038899895.1	1.1e-44	86.21	DNA polymerase I isoform X4 [Benincasa hispida]	[more]
XP_038899897.1	1.1e-44	86.21	DNA polymerase I isoform X6 [Benincasa hispida]	[more]
XP_022933106.1	1.5e-44	85.47	uncharacterized protein LOC111439872 isoform X1 [Cucurbita moschata]	[more]

BLAST of Spg006800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O52225	2.2e-14	40.57	DNA polymerase I, thermostable OS=Thermus filiformis OX=276 GN=polA PE=1 SV=1	[more]
P30313	8.2e-14	39.62	DNA polymerase I, thermostable OS=Thermus thermophilus OX=274 GN=polA PE=1 SV=1	[more]
Q55971	4.1e-13	43.75	DNA polymerase I OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=p...	[more]
P52028	5.3e-13	38.68	DNA polymerase I, thermostable OS=Thermus thermophilus (strain ATCC 27634 / DSM ...	[more]
P00582	5.3e-13	43.18	DNA polymerase I OS=Escherichia coli (strain K12) OX=83333 GN=polA PE=1 SV=1	[more]

BLAST of Spg006800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HVF9	7.1e-45	85.47	DNA-directed DNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111467206 PE=4 SV=...	[more]
A0A6J1EY78	7.1e-45	85.47	DNA-directed DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111439872 PE=4 S...	[more]
A0A6J1F3S3	9.3e-45	86.21	DNA-directed DNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111439872 PE=4 S...	[more]
A0A6J1D5H7	5.1e-43	83.76	DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 ...	[more]
A0A6J1D7Q6	5.1e-43	84.48	DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 ...	[more]

BLAST of Spg006800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52050.2	2.3e-43	77.88	5'-3' exonuclease family protein	[more]
AT3G52050.1	2.3e-43	77.88	5'-3' exonuclease family protein	[more]
AT3G52050.3	2.3e-43	77.88	5'-3' exonuclease family protein	[more]
AT3G52050.4	2.3e-43	77.88	5'-3' exonuclease family protein	[more]
AT3G52050.5	1.1e-42	79.82	5'-3' exonuclease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002421	5'-3' exonuclease	SMART	SM00475	53exo3	coord: 2..203 e-value: 1.4E-8 score: -5.5
IPR020046	5'-3' exonuclease, alpha-helical arch, N-terminal	PFAM	PF02739	5_3_exonuc_N	coord: 12..103 e-value: 1.3E-27 score: 96.6
None	No IPR available	GENE3D	3.40.50.1010		coord: 6..119 e-value: 1.2E-30 score: 108.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR10133:SF54	5'-3' EXONUCLEASE FAMILY PROTEIN	coord: 9..109
None	No IPR available	CDD	cd09859	PIN_53EXO	coord: 12..99 e-value: 2.09164E-38 score: 129.022
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 107..152 e-value: 1.1E-6 score: 29.3
IPR002298	DNA polymerase A	PANTHER	PTHR10133	DNA POLYMERASE I	coord: 9..109
IPR029060	PIN-like domain superfamily	SUPERFAMILY	88723	PIN domain-like	coord: 11..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg006800.1	Spg006800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0006261	DNA-dependent DNA replication
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0016787	hydrolase activity