Spg006454 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg006454 vs. NCBI nr
Match: XP_022961393.1 (calmodulin-binding protein 60 G-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 327.4 bits (838), Expect = 1.7e-85
Identity = 172/334 (51.50%), Postives = 232/334 (69.46%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           V  +V   +  ++S     ++ KEE   G G SK+KL FLNQ   +IFTNN++EA+NG+P
Sbjct: 65  VELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFTNNEIEARNGKP 124

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L +A+C+ TN N II+ GPLSSA +E VVL+ EFGS ++ +E  W+ +DFN +V+S+R+G
Sbjct: 125 LLVAMCDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQRVDESSWTSSDFNNNVVSKREG 184

Query: 177 KRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAV 236
           KRPL+VG D+ + +KNGV  IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAV
Sbjct: 185 KRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAV 244

Query: 237 SQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEK 296
           S+PFRV DHRGEV KKHHPP  EDE+WRL+GIGK+GVYH  LSS  I+ VDDFLN + +K
Sbjct: 245 SKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGIKNVDDFLNAYHKK 304

Query: 297 GEVFLRKLLGKRVPDRVWNAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEEN 356
           G +++RKLLG RVP   W  MV NA E   F  + ++   F++  + GNE M  EAF + 
Sbjct: 305 GPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVCSM-GNEAMGGEAF-DM 364

Query: 357 NAEVGEQKINLSDGDELHQILSGFGQELESMLDF 389
           N+EV E K N S+ D L      F  +L+ ++ +
Sbjct: 365 NSEVSEPK-NCSESDGLQ-----FDGQLQDLVSY 390

BLAST of Spg006454 vs. NCBI nr
Match: XP_022961394.1 (calmodulin-binding protein 60 G-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 327.4 bits (838), Expect = 1.7e-85
Identity = 172/334 (51.50%), Postives = 232/334 (69.46%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           V  +V   +  ++S     ++ KEE   G G SK+KL FLNQ   +IFTNN++EA+NG+P
Sbjct: 6   VELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFTNNEIEARNGKP 65

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L +A+C+ TN N II+ GPLSSA +E VVL+ EFGS ++ +E  W+ +DFN +V+S+R+G
Sbjct: 66  LLVAMCDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQRVDESSWTSSDFNNNVVSKREG 125

Query: 177 KRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAV 236
           KRPL+VG D+ + +KNGV  IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAV
Sbjct: 126 KRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAV 185

Query: 237 SQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEK 296
           S+PFRV DHRGEV KKHHPP  EDE+WRL+GIGK+GVYH  LSS  I+ VDDFLN + +K
Sbjct: 186 SKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGIKNVDDFLNAYHKK 245

Query: 297 GEVFLRKLLGKRVPDRVWNAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEEN 356
           G +++RKLLG RVP   W  MV NA E   F  + ++   F++  + GNE M  EAF + 
Sbjct: 246 GPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVCSM-GNEAMGGEAF-DM 305

Query: 357 NAEVGEQKINLSDGDELHQILSGFGQELESMLDF 389
           N+EV E K N S+ D L      F  +L+ ++ +
Sbjct: 306 NSEVSEPK-NCSESDGLQ-----FDGQLQDLVSY 331

BLAST of Spg006454 vs. NCBI nr
Match: XP_022968714.1 (calmodulin-binding protein 60 G-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 323.9 bits (829), Expect = 1.9e-84
Identity = 170/334 (50.90%), Postives = 229/334 (68.56%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           V  +V   +  ++S    N++ KE    G G SK+KL FLNQ   +IFTNN++EA+NG+P
Sbjct: 65  VELQVNYYIRSILSSQLSNHIGKEATINGFGASKFKLLFLNQTAPTIFTNNEIEARNGKP 124

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L +AIC+ TN N II+ GPLSSA +E VVL+ EFGS ++ +E  W+  DFN +V+S+R+G
Sbjct: 125 LLVAICDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQRVDESSWTSRDFNNNVVSKREG 184

Query: 177 KRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAV 236
           KRPL+VG D+ + +KNGV  IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAV
Sbjct: 185 KRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAV 244

Query: 237 SQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEK 296
           S+PFRV DHRG+V KKHHPPR EDE+WRL+GIGK+G+YH  L S  I+ VDDFL  + +K
Sbjct: 245 SKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSKGIKNVDDFLKAYHKK 304

Query: 297 GEVFLRKLLGKRVPDRVWNAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEEN 356
           G  + RKLLG RVP   W  MV NA E   F  + ++   F+++ + GNE M  EAF + 
Sbjct: 305 GPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVRSM-GNETMGGEAF-DM 364

Query: 357 NAEVGEQKINLSDGDELHQILSGFGQELESMLDF 389
           N+EV E K N S+ D L      F  +L+ ++ +
Sbjct: 365 NSEVSEPK-NYSESDGLQ-----FDGQLQDLVSY 390

BLAST of Spg006454 vs. NCBI nr
Match: XP_022968713.1 (calmodulin-binding protein 60 G-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 321.6 bits (823), Expect = 9.3e-84
Identity = 167/316 (52.85%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 75  NNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEPLQLAICEVTNFNTIIRMG 134
           N++ KE    G G SK+KL FLNQ   +IFTNN++EA+NG+PL +AIC+ TN N II+ G
Sbjct: 85  NHIGKEATINGFGASKFKLLFLNQTAPTIFTNNEIEARNGKPLLVAICDTTNSNGIIKTG 144

Query: 135 PLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDGKRPLLVGKDLNLGMKNGV 194
           PLSSA +E VVL+ EFGS ++ +E  W+  DFN +V+S+R+GKRPL+VG D+ + +KNGV
Sbjct: 145 PLSSALVELVVLNLEFGSDQRVDESSWTSRDFNNNVVSKREGKRPLIVGNDMIICLKNGV 204

Query: 195 AVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAVSQPFRVKDHRGEVNKKHH 254
             IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAVS+PFRV DHRG+V KKHH
Sbjct: 205 GFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHH 264

Query: 255 PPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEKGEVFLRKLLGKRVPDRVW 314
           PPR EDE+WRL+GIGK+G+YH  L S  I+ VDDFL  + +KG  + RKLLG RVP   W
Sbjct: 265 PPRREDEIWRLEGIGKDGIYHNRLFSKGIKNVDDFLKAYHKKGPTYFRKLLGGRVPQNTW 324

Query: 315 NAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEENNAEVGEQKINLSDGDELH 374
             MV NA E   F  + ++   F+++ + GNE M  EAF + N+EV E K N S+ D L 
Sbjct: 325 KRMVNNALECDAFIASMASNPSFRVRSM-GNETMGGEAF-DMNSEVSEPK-NYSESDGLQ 384

Query: 375 QILSGFGQELESMLDF 389
                F  +L+ ++ +
Sbjct: 385 -----FDGQLQDLVSY 392

BLAST of Spg006454 vs. NCBI nr
Match: XP_008450592.1 (PREDICTED: calmodulin-binding protein 60 G-like [Cucumis melo] >XP_008450593.1 PREDICTED: calmodulin-binding protein 60 G-like [Cucumis melo] >KAA0050926.1 calmodulin-binding protein 60 G-like [Cucumis melo var. makuwa] >TYK10274.1 calmodulin-binding protein 60 G-like [Cucumis melo var. makuwa])

HSP 1 Score: 316.6 bits (810), Expect = 3.0e-82
Identity = 172/382 (45.03%), Postives = 234/382 (61.26%), Query Frame = 0

Query: 9   KKRLFPYLEESQNLKKSS-------HSFPTDFGAQSSICYKELALVLKNLISCPKV---- 68
           K+R + Y  E+ N++  S        +FPT F  +      +     ++LI  P++    
Sbjct: 11  KRRRYSY--EATNVQDDSIAPLPGHFTFPTSFYTRREPVIDDAVTRSRSLIEEPRIGEVV 70

Query: 69  ---------------REEVEKKVEELI----------SLSQPNNLTKEEDAAGSGTSKYK 128
                          R++V K++E ++          SLS      +EE    S T K+K
Sbjct: 71  KRFYDEIWPKMEHDFRQQVSKEIERMVHSAIHSAFSPSLSIQQGCEQEEILKPSKTLKFK 130

Query: 129 LCFLNQIPSSIFTNNDLEAQNGEPLQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEF-G 188
           LCFLN  PS IFTNN++++++GEPL++AIC+ TN N+I+  GPLSSAQ+E  +L GEF  
Sbjct: 131 LCFLNSFPSVIFTNNEVKSEDGEPLKVAICDATNSNSIVSTGPLSSAQVELCILPGEFDS 190

Query: 189 SGRKKEEVHWSVTDFNTSVLSQRDGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKS 248
           S R+ EE  W+ + FN S+L+ RDGKRPL++G D  + +K+GV +I NL  TDNSSW+KS
Sbjct: 191 SNRRDEETPWTSSYFNKSILTPRDGKRPLIIGDDRQVYLKDGVRLINNLIITDNSSWMKS 250

Query: 249 KKFRLGARIVDEKNLGEFTRIGEAVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKN 308
           KKFRLG +I DE+ L EF RIGEA+S+PFRV D RGEVN+KHHPPR EDEVWRL+GI K+
Sbjct: 251 KKFRLGVKIKDERVLAEFVRIGEAISEPFRVMDQRGEVNQKHHPPRWEDEVWRLEGIAKD 310

Query: 309 GVYHKNLSSNDIQTVDDFLNVHFEKGEVFLRKLLGKRVPDRVWNAMVKNASERFSINDSN 354
           G+YHKNLSS  I+TV DFL  + +     LR +LGKRV D+ W  MV+NA E       N
Sbjct: 311 GIYHKNLSSKGIRTVGDFLKAYHQNDPDTLRMMLGKRVLDKTWKMMVQNACECVEDTFDN 370

BLAST of Spg006454 vs. ExPASy Swiss-Prot
Match: F4K2R6 (Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.0e-48
Identity = 109/269 (40.52%), Postives = 164/269 (60.97%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           VREE+++ ++  +S S   ++ +      S  S+ KLCF+N  PSSIFT + +EA++G P
Sbjct: 53  VREEIQRSLQPFLS-SSCVSMERSRSETPSSRSRLKLCFINSPPSSIFTGSKIEAEDGSP 112

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L + + + T  NT++  GP SS+++E V L+ +F       E  W+V  FN ++L+QR+G
Sbjct: 113 LVIELVDATT-NTLVSTGPFSSSRVELVPLNADF------TEESWTVEGFNRNILTQREG 172

Query: 177 KRPLLVGKDLNLGMKNGVAVIK-NLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEA 236
           KRPLL G DL + +KNGV VI  ++ F+DNSSW +S+KFRLGA++  +  +       EA
Sbjct: 173 KRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGAV-------EA 232

Query: 237 VSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVH-F 296
            S+ F  +D RGE  KKHHPP   DEVWRL+ I K+GV    L+   I TV DF  ++  
Sbjct: 233 RSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILTVKDFRRLYTV 292

Query: 297 EKGEVFLRKLLGKRVPDRVWNAMVKNASE 324
            + E  L  ++G  V  + WN +V +A +
Sbjct: 293 NRNE--LHNIIGAGVSKKTWNTIVSHAMD 303

BLAST of Spg006454 vs. ExPASy Swiss-Prot
Match: Q9C9T2 (Protein SAR DEFICIENT 1 OS=Arabidopsis thaliana OX=3702 GN=SARD1 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.3e-45
Identity = 116/322 (36.02%), Postives = 182/322 (56.52%), Query Frame = 0

Query: 10  KRLFPYLEESQNLKKS--------SHSFPTDFGAQSSICYKELALVLKNLISCPKVREEV 69
           KRLF  L+  Q  K          S + P      + I    L  VL+ +I    VR+EV
Sbjct: 4   KRLFQDLDSDQENKSEKRIKSVLPSLASPISSVFGALISENTLRSVLEPVIR-KVVRQEV 63

Query: 70  EKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEPLQLAI 129
           E  + +   LS+ ++   E  A  + T   KL F   + + IFT + +   +  PL++ +
Sbjct: 64  EYGISKRFRLSRSSSFRIE--APEATTPTLKLIFRKNLMTPIFTGSKISDVDNNPLEIIL 123

Query: 130 CEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDGKRPLL 189
            + +N    +        +++ V L G+F SG K     W+  +F ++++ +RDGKRPLL
Sbjct: 124 VDDSNKPVNLN----RPIKLDIVALHGDFPSGDK-----WTSDEFESNIIKERDGKRPLL 183

Query: 190 VGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAVSQPFR 249
            G ++++ ++NGVA I  + FTDNSSW++S+KFR+GA++  + + G+   + EA+++   
Sbjct: 184 AG-EVSVTVRNGVATIGEIVFTDNSSWIRSRKFRIGAKVA-KGSSGQGVVVCEAMTEAIV 243

Query: 250 VKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEKGEVFL 309
           V+DHRGE+ KKHHPP  EDEVWRL+ IGK+G +HK LSS  I TV DFL +     +  L
Sbjct: 244 VRDHRGELYKKHHPPMLEDEVWRLEKIGKDGAFHKKLSSRHINTVQDFLKLSVVDVDE-L 303

Query: 310 RKLLGKRVPDRVWNAMVKNASE 324
           R++LG  + DR W   +K+A E
Sbjct: 304 RQILGPGMSDRKWEVTLKHARE 310

BLAST of Spg006454 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.0e-45
Identity = 100/272 (36.76%), Postives = 164/272 (60.29%), Query Frame = 0

Query: 55  PKVREEVEKKVEELISLSQPNNLTKEEDAA-----GSGTSKYKLCFLNQIPSSIFTNNDL 114
           P +R  V +++E  ++   P  LT    ++     G    K +L F +++   +FT   +
Sbjct: 53  PILRRVVSEELERALAKLGPARLTGSSGSSPKRIEGPDGRKLQLHFKSRLSLPLFTGGKV 112

Query: 115 EAQNGEPLQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTS 174
           E + G  + + + +      ++  GP +SA++  VVL+G+F +   +++  W+  +F + 
Sbjct: 113 EGEQGAVIHVVLIDANTGRAVV-YGPEASAKLHIVVLEGDFNT---EDDEDWTQEEFESH 172

Query: 175 VLSQRDGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEF 234
           V+ +R GKRPLL G ++ + +K GV  +  L FTDNSSW++S+KFRLG R+V      + 
Sbjct: 173 VVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSSWIRSRKFRLGLRVV--SGCCDG 232

Query: 235 TRIGEAVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDF 294
            RI EA ++ F VKDHRGE+ KKH+PP   D+VWRLD IGK+G +HK L++  I TV+DF
Sbjct: 233 MRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDKIGKDGAFHKKLTAEGINTVEDF 292

Query: 295 LNVHFEKGEVFLRKLLGKRVPDRVWNAMVKNA 322
           L V   K    LR +LG  + +++W+A+V++A
Sbjct: 293 LRV-MVKDSPKLRTILGSGMSNKMWDALVEHA 316

BLAST of Spg006454 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-44
Identity = 105/310 (33.87%), Postives = 178/310 (57.42%), Query Frame = 0

Query: 32  DFGAQSSICYKELALVLKNLISCPKVREEVEKKVEELISLSQPNNLTK-----EEDAAGS 91
           DFG++ S+  + + L        P +   + K V+E + L+   +L       E++    
Sbjct: 7   DFGSKFSVVQEVMRLQTVKHFLEPVLEPLIRKVVKEEVELALGKHLAGIKWICEKETHPL 66

Query: 92  GTSKYKLCFLNQIPSSIFTNNDLEAQNGEPLQLAICEVTNFNTIIRMGPLSSAQIEFVVL 151
            +   +L FLN +   +FT+  +E   G+ +++ + + +    I   GP SSA++E  V+
Sbjct: 67  ESRNLQLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPST-GQIFSSGPASSAKLEVFVV 126

Query: 152 DGEFGSGRKKEEVHWSVTDFNTSVLSQRDGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNS 211
           +G+F S        W+  D   +++ +R+GK+PLL G    + + +G+ V+  + FTDNS
Sbjct: 127 EGDFNS-----VSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNS 186

Query: 212 SWLKSKKFRLGARIVDEKNLGEFTRIGEAVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLD 271
           SW +S+KFRLG RIVD+    ++ +I EA+++ F V+DHRGE+ KKHHPP   DEVWRL+
Sbjct: 187 SWTRSRKFRLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLE 246

Query: 272 GIGKNGVYHKNLSSNDIQTVDDFLNVHFEKGEVFLRKLLGKRVPDRVWNAMVKNASERFS 331
            IGK+G +H+ L+ ++I TV DFL  HF      LR++LG  +  ++W   + +A  R  
Sbjct: 247 KIGKDGAFHRRLNLSNINTVKDFL-THFHLNSSKLRQVLGTGMSSKMWEITLDHA--RSC 303

Query: 332 INDSNPNGFQ 337
           + DS+ + +Q
Sbjct: 307 VLDSSVHVYQ 303

BLAST of Spg006454 vs. ExPASy Swiss-Prot
Match: C0SV51 (Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.8e-43
Identity = 108/327 (33.03%), Postives = 182/327 (55.66%), Query Frame = 0

Query: 1   MEKGRAQYKKRLFPYLEESQNLKKSSHSFPTDFGAQSSICYKELAL-VLKNLISC--PKV 60
           ME+  +  +KR     +  Q  ++     P    A +S+  + L +  L+ L S   P +
Sbjct: 6   MERTNSMREKRKLEEDDNQQQQQQPERKRP----ALASVIVEALKMDSLQRLCSSLEPIL 65

Query: 61  REEVEKKVEELISLSQPNNLTKEED---AAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNG 120
           R  V ++VE  ++   P  L++        G G    +L F +++   +FT   +E + G
Sbjct: 66  RRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTGGKIEGEQG 125

Query: 121 EPLQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQR 180
             + + + ++T    ++ +GP +SA+++ VVLDG+F +   +++  WS  +F   ++ +R
Sbjct: 126 AAIHVVLLDMTT-GHVLTVGPEASAKLDVVVLDGDFNT---EDDDGWSGEEFEGHLVKER 185

Query: 181 DGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGE 240
            GKRPLL G D+ + +K GV  +  L FTDNSSW++ +KFRLG R+       E  R+ E
Sbjct: 186 QGKRPLLTG-DVQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRV--SSGYCEGMRVRE 245

Query: 241 AVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHF 300
           A ++ F VKDHRGE+ KKH+PP  +DEVWRL+ IGK+G +HK L+   I  V +FL +  
Sbjct: 246 AKTEAFTVKDHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFLRL-M 305

Query: 301 EKGEVFLRKLLGKRVPDRVWNAMVKNA 322
            K    LR +LG  + +R+W  + +++
Sbjct: 306 VKDSQKLRTILGSGMSNRMWETLAEHS 320

BLAST of Spg006454 vs. ExPASy TrEMBL
Match: A0A6J1HBN4 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461979 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 8.2e-86
Identity = 172/334 (51.50%), Postives = 232/334 (69.46%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           V  +V   +  ++S     ++ KEE   G G SK+KL FLNQ   +IFTNN++EA+NG+P
Sbjct: 65  VELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFTNNEIEARNGKP 124

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L +A+C+ TN N II+ GPLSSA +E VVL+ EFGS ++ +E  W+ +DFN +V+S+R+G
Sbjct: 125 LLVAMCDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQRVDESSWTSSDFNNNVVSKREG 184

Query: 177 KRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAV 236
           KRPL+VG D+ + +KNGV  IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAV
Sbjct: 185 KRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAV 244

Query: 237 SQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEK 296
           S+PFRV DHRGEV KKHHPP  EDE+WRL+GIGK+GVYH  LSS  I+ VDDFLN + +K
Sbjct: 245 SKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGIKNVDDFLNAYHKK 304

Query: 297 GEVFLRKLLGKRVPDRVWNAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEEN 356
           G +++RKLLG RVP   W  MV NA E   F  + ++   F++  + GNE M  EAF + 
Sbjct: 305 GPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVCSM-GNEAMGGEAF-DM 364

Query: 357 NAEVGEQKINLSDGDELHQILSGFGQELESMLDF 389
           N+EV E K N S+ D L      F  +L+ ++ +
Sbjct: 365 NSEVSEPK-NCSESDGLQ-----FDGQLQDLVSY 390

BLAST of Spg006454 vs. ExPASy TrEMBL
Match: A0A6J1HDW8 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461979 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 8.2e-86
Identity = 172/334 (51.50%), Postives = 232/334 (69.46%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           V  +V   +  ++S     ++ KEE   G G SK+KL FLNQ   +IFTNN++EA+NG+P
Sbjct: 6   VELQVNYYIHSILSSQLSIHIVKEETINGFGASKFKLLFLNQTSPTIFTNNEIEARNGKP 65

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L +A+C+ TN N II+ GPLSSA +E VVL+ EFGS ++ +E  W+ +DFN +V+S+R+G
Sbjct: 66  LLVAMCDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQRVDESSWTSSDFNNNVVSKREG 125

Query: 177 KRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAV 236
           KRPL+VG D+ + +KNGV  IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAV
Sbjct: 126 KRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAV 185

Query: 237 SQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEK 296
           S+PFRV DHRGEV KKHHPP  EDE+WRL+GIGK+GVYH  LSS  I+ VDDFLN + +K
Sbjct: 186 SKPFRVMDHRGEVYKKHHPPSREDEIWRLEGIGKDGVYHNRLSSEGIKNVDDFLNAYHKK 245

Query: 297 GEVFLRKLLGKRVPDRVWNAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEEN 356
           G +++RKLLG RVP   W  MV NA E   F  + ++   F++  + GNE M  EAF + 
Sbjct: 246 GPIYVRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVCSM-GNEAMGGEAF-DM 305

Query: 357 NAEVGEQKINLSDGDELHQILSGFGQELESMLDF 389
           N+EV E K N S+ D L      F  +L+ ++ +
Sbjct: 306 NSEVSEPK-NCSESDGLQ-----FDGQLQDLVSY 331

BLAST of Spg006454 vs. ExPASy TrEMBL
Match: A0A6J1I0G5 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 9.1e-85
Identity = 170/334 (50.90%), Postives = 229/334 (68.56%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           V  +V   +  ++S    N++ KE    G G SK+KL FLNQ   +IFTNN++EA+NG+P
Sbjct: 65  VELQVNYYIRSILSSQLSNHIGKEATINGFGASKFKLLFLNQTAPTIFTNNEIEARNGKP 124

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L +AIC+ TN N II+ GPLSSA +E VVL+ EFGS ++ +E  W+  DFN +V+S+R+G
Sbjct: 125 LLVAICDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQRVDESSWTSRDFNNNVVSKREG 184

Query: 177 KRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAV 236
           KRPL+VG D+ + +KNGV  IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAV
Sbjct: 185 KRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAV 244

Query: 237 SQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEK 296
           S+PFRV DHRG+V KKHHPPR EDE+WRL+GIGK+G+YH  L S  I+ VDDFL  + +K
Sbjct: 245 SKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSKGIKNVDDFLKAYHKK 304

Query: 297 GEVFLRKLLGKRVPDRVWNAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEEN 356
           G  + RKLLG RVP   W  MV NA E   F  + ++   F+++ + GNE M  EAF + 
Sbjct: 305 GPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVRSM-GNETMGGEAF-DM 364

Query: 357 NAEVGEQKINLSDGDELHQILSGFGQELESMLDF 389
           N+EV E K N S+ D L      F  +L+ ++ +
Sbjct: 365 NSEVSEPK-NYSESDGLQ-----FDGQLQDLVSY 390

BLAST of Spg006454 vs. ExPASy TrEMBL
Match: A0A6J1HYX2 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 4.5e-84
Identity = 167/316 (52.85%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 75  NNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEPLQLAICEVTNFNTIIRMG 134
           N++ KE    G G SK+KL FLNQ   +IFTNN++EA+NG+PL +AIC+ TN N II+ G
Sbjct: 85  NHIGKEATINGFGASKFKLLFLNQTAPTIFTNNEIEARNGKPLLVAICDTTNSNGIIKTG 144

Query: 135 PLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDGKRPLLVGKDLNLGMKNGV 194
           PLSSA +E VVL+ EFGS ++ +E  W+  DFN +V+S+R+GKRPL+VG D+ + +KNGV
Sbjct: 145 PLSSALVELVVLNLEFGSDQRVDESSWTSRDFNNNVVSKREGKRPLIVGNDMIICLKNGV 204

Query: 195 AVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAVSQPFRVKDHRGEVNKKHH 254
             IK L F+DNSSW KSKKFRLGA++VD+  L +F RI EAVS+PFRV DHRG+V KKHH
Sbjct: 205 GFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHH 264

Query: 255 PPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEKGEVFLRKLLGKRVPDRVW 314
           PPR EDE+WRL+GIGK+G+YH  L S  I+ VDDFL  + +KG  + RKLLG RVP   W
Sbjct: 265 PPRREDEIWRLEGIGKDGIYHNRLFSKGIKNVDDFLKAYHKKGPTYFRKLLGGRVPQNTW 324

Query: 315 NAMVKNASE--RFSINDSNPNGFQMQDLVGNEEMRVEAFEENNAEVGEQKINLSDGDELH 374
             MV NA E   F  + ++   F+++ + GNE M  EAF + N+EV E K N S+ D L 
Sbjct: 325 KRMVNNALECDAFIASMASNPSFRVRSM-GNETMGGEAF-DMNSEVSEPK-NYSESDGLQ 384

Query: 375 QILSGFGQELESMLDF 389
                F  +L+ ++ +
Sbjct: 385 -----FDGQLQDLVSY 392

BLAST of Spg006454 vs. ExPASy TrEMBL
Match: A0A1S3BQ76 (calmodulin-binding protein 60 G-like OS=Cucumis melo OX=3656 GN=LOC103492135 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 1.4e-82
Identity = 172/382 (45.03%), Postives = 234/382 (61.26%), Query Frame = 0

Query: 9   KKRLFPYLEESQNLKKSS-------HSFPTDFGAQSSICYKELALVLKNLISCPKV---- 68
           K+R + Y  E+ N++  S        +FPT F  +      +     ++LI  P++    
Sbjct: 11  KRRRYSY--EATNVQDDSIAPLPGHFTFPTSFYTRREPVIDDAVTRSRSLIEEPRIGEVV 70

Query: 69  ---------------REEVEKKVEELI----------SLSQPNNLTKEEDAAGSGTSKYK 128
                          R++V K++E ++          SLS      +EE    S T K+K
Sbjct: 71  KRFYDEIWPKMEHDFRQQVSKEIERMVHSAIHSAFSPSLSIQQGCEQEEILKPSKTLKFK 130

Query: 129 LCFLNQIPSSIFTNNDLEAQNGEPLQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEF-G 188
           LCFLN  PS IFTNN++++++GEPL++AIC+ TN N+I+  GPLSSAQ+E  +L GEF  
Sbjct: 131 LCFLNSFPSVIFTNNEVKSEDGEPLKVAICDATNSNSIVSTGPLSSAQVELCILPGEFDS 190

Query: 189 SGRKKEEVHWSVTDFNTSVLSQRDGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKS 248
           S R+ EE  W+ + FN S+L+ RDGKRPL++G D  + +K+GV +I NL  TDNSSW+KS
Sbjct: 191 SNRRDEETPWTSSYFNKSILTPRDGKRPLIIGDDRQVYLKDGVRLINNLIITDNSSWMKS 250

Query: 249 KKFRLGARIVDEKNLGEFTRIGEAVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKN 308
           KKFRLG +I DE+ L EF RIGEA+S+PFRV D RGEVN+KHHPPR EDEVWRL+GI K+
Sbjct: 251 KKFRLGVKIKDERVLAEFVRIGEAISEPFRVMDQRGEVNQKHHPPRWEDEVWRLEGIAKD 310

Query: 309 GVYHKNLSSNDIQTVDDFLNVHFEKGEVFLRKLLGKRVPDRVWNAMVKNASERFSINDSN 354
           G+YHKNLSS  I+TV DFL  + +     LR +LGKRV D+ W  MV+NA E       N
Sbjct: 311 GIYHKNLSSKGIRTVGDFLKAYHQNDPDTLRMMLGKRVLDKTWKMMVQNACECVEDTFDN 370

BLAST of Spg006454 vs. TAIR 10
Match: AT5G26920.1 (Cam-binding protein 60-like G )

HSP 1 Score: 193.4 bits (490), Expect = 3.6e-49
Identity = 109/269 (40.52%), Postives = 164/269 (60.97%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           VREE+++ ++  +S S   ++ +      S  S+ KLCF+N  PSSIFT + +EA++G P
Sbjct: 53  VREEIQRSLQPFLS-SSCVSMERSRSETPSSRSRLKLCFINSPPSSIFTGSKIEAEDGSP 112

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L + + + T  NT++  GP SS+++E V L+ +F       E  W+V  FN ++L+QR+G
Sbjct: 113 LVIELVDATT-NTLVSTGPFSSSRVELVPLNADF------TEESWTVEGFNRNILTQREG 172

Query: 177 KRPLLVGKDLNLGMKNGVAVIK-NLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEA 236
           KRPLL G DL + +KNGV VI  ++ F+DNSSW +S+KFRLGA++  +  +       EA
Sbjct: 173 KRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGAV-------EA 232

Query: 237 VSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVH-F 296
            S+ F  +D RGE  KKHHPP   DEVWRL+ I K+GV    L+   I TV DF  ++  
Sbjct: 233 RSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILTVKDFRRLYTV 292

Query: 297 EKGEVFLRKLLGKRVPDRVWNAMVKNASE 324
            + E  L  ++G  V  + WN +V +A +
Sbjct: 293 NRNE--LHNIIGAGVSKKTWNTIVSHAMD 303

BLAST of Spg006454 vs. TAIR 10
Match: AT5G26920.2 (Cam-binding protein 60-like G )

HSP 1 Score: 190.7 bits (483), Expect = 2.3e-48
Identity = 107/268 (39.93%), Postives = 161/268 (60.07%), Query Frame = 0

Query: 57  VREEVEKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEP 116
           VREE+++ ++  +S S   ++ +      S  S+ KLCF+N  PSSIFT + +EA++G P
Sbjct: 53  VREEIQRSLQPFLS-SSCVSMERSRSETPSSRSRLKLCFINSPPSSIFTGSKIEAEDGSP 112

Query: 117 LQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDG 176
           L + + + T  NT++  GP SS+++E V L+ +F       E  W+V  FN ++L+QR+G
Sbjct: 113 LVIELVDATT-NTLVSTGPFSSSRVELVPLNADF------TEESWTVEGFNRNILTQREG 172

Query: 177 KRPLLVGKDLNLGMKNGVAVIK-NLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEA 236
           KRPLL G DL + +KNGV VI  ++ F+DNSSW +S+KFRLGA++  +  +       EA
Sbjct: 173 KRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKFRLGAKLTGDGAV-------EA 232

Query: 237 VSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFE 296
            S+ F  +D RGE  KKHHPP   DEVWRL+ I K+GV    L+   I TV DF  ++  
Sbjct: 233 RSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVSATRLAERKILTVKDFRRLY-- 292

Query: 297 KGEVFLRKLLGKRVPDRVWNAMVKNASE 324
                   ++G  V  + WN +V +A +
Sbjct: 293 -------TIIGAGVSKKTWNTIVSHAMD 295

BLAST of Spg006454 vs. TAIR 10
Match: AT1G73805.1 (Calmodulin binding protein-like )

HSP 1 Score: 184.5 bits (467), Expect = 1.7e-46
Identity = 116/322 (36.02%), Postives = 182/322 (56.52%), Query Frame = 0

Query: 10  KRLFPYLEESQNLKKS--------SHSFPTDFGAQSSICYKELALVLKNLISCPKVREEV 69
           KRLF  L+  Q  K          S + P      + I    L  VL+ +I    VR+EV
Sbjct: 4   KRLFQDLDSDQENKSEKRIKSVLPSLASPISSVFGALISENTLRSVLEPVIR-KVVRQEV 63

Query: 70  EKKVEELISLSQPNNLTKEEDAAGSGTSKYKLCFLNQIPSSIFTNNDLEAQNGEPLQLAI 129
           E  + +   LS+ ++   E  A  + T   KL F   + + IFT + +   +  PL++ +
Sbjct: 64  EYGISKRFRLSRSSSFRIE--APEATTPTLKLIFRKNLMTPIFTGSKISDVDNNPLEIIL 123

Query: 130 CEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTSVLSQRDGKRPLL 189
            + +N    +        +++ V L G+F SG K     W+  +F ++++ +RDGKRPLL
Sbjct: 124 VDDSNKPVNLN----RPIKLDIVALHGDFPSGDK-----WTSDEFESNIIKERDGKRPLL 183

Query: 190 VGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEFTRIGEAVSQPFR 249
            G ++++ ++NGVA I  + FTDNSSW++S+KFR+GA++  + + G+   + EA+++   
Sbjct: 184 AG-EVSVTVRNGVATIGEIVFTDNSSWIRSRKFRIGAKVA-KGSSGQGVVVCEAMTEAIV 243

Query: 250 VKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDFLNVHFEKGEVFL 309
           V+DHRGE+ KKHHPP  EDEVWRL+ IGK+G +HK LSS  I TV DFL +     +  L
Sbjct: 244 VRDHRGELYKKHHPPMLEDEVWRLEKIGKDGAFHKKLSSRHINTVQDFLKLSVVDVDE-L 303

Query: 310 RKLLGKRVPDRVWNAMVKNASE 324
           R++LG  + DR W   +K+A E
Sbjct: 304 RQILGPGMSDRKWEVTLKHARE 310

BLAST of Spg006454 vs. TAIR 10
Match: AT5G57580.1 (Calmodulin-binding protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.2e-46
Identity = 100/272 (36.76%), Postives = 164/272 (60.29%), Query Frame = 0

Query: 55  PKVREEVEKKVEELISLSQPNNLTKEEDAA-----GSGTSKYKLCFLNQIPSSIFTNNDL 114
           P +R  V +++E  ++   P  LT    ++     G    K +L F +++   +FT   +
Sbjct: 53  PILRRVVSEELERALAKLGPARLTGSSGSSPKRIEGPDGRKLQLHFKSRLSLPLFTGGKV 112

Query: 115 EAQNGEPLQLAICEVTNFNTIIRMGPLSSAQIEFVVLDGEFGSGRKKEEVHWSVTDFNTS 174
           E + G  + + + +      ++  GP +SA++  VVL+G+F +   +++  W+  +F + 
Sbjct: 113 EGEQGAVIHVVLIDANTGRAVV-YGPEASAKLHIVVLEGDFNT---EDDEDWTQEEFESH 172

Query: 175 VLSQRDGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNSSWLKSKKFRLGARIVDEKNLGEF 234
           V+ +R GKRPLL G ++ + +K GV  +  L FTDNSSW++S+KFRLG R+V      + 
Sbjct: 173 VVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSSWIRSRKFRLGLRVV--SGCCDG 232

Query: 235 TRIGEAVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLDGIGKNGVYHKNLSSNDIQTVDDF 294
            RI EA ++ F VKDHRGE+ KKH+PP   D+VWRLD IGK+G +HK L++  I TV+DF
Sbjct: 233 MRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDKIGKDGAFHKKLTAEGINTVEDF 292

Query: 295 LNVHFEKGEVFLRKLLGKRVPDRVWNAMVKNA 322
           L V   K    LR +LG  + +++W+A+V++A
Sbjct: 293 LRV-MVKDSPKLRTILGSGMSNKMWDALVEHA 316

BLAST of Spg006454 vs. TAIR 10
Match: AT5G62570.2 (Calmodulin binding protein-like )

HSP 1 Score: 181.8 bits (460), Expect = 1.1e-45
Identity = 105/310 (33.87%), Postives = 178/310 (57.42%), Query Frame = 0

Query: 32  DFGAQSSICYKELALVLKNLISCPKVREEVEKKVEELISLSQPNNLTK-----EEDAAGS 91
           DFG++ S+  + + L        P +   + K V+E + L+   +L       E++    
Sbjct: 7   DFGSKFSVVQEVMRLQTVKHFLEPVLEPLIRKVVKEEVELALGKHLAGIKWICEKETHPL 66

Query: 92  GTSKYKLCFLNQIPSSIFTNNDLEAQNGEPLQLAICEVTNFNTIIRMGPLSSAQIEFVVL 151
            +   +L FLN +   +FT+  +E   G+ +++ + + +    I   GP SSA++E  V+
Sbjct: 67  ESRNLQLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPST-GQIFSSGPASSAKLEVFVV 126

Query: 152 DGEFGSGRKKEEVHWSVTDFNTSVLSQRDGKRPLLVGKDLNLGMKNGVAVIKNLFFTDNS 211
           +G+F S        W+  D   +++ +R+GK+PLL G    + + +G+ V+  + FTDNS
Sbjct: 127 EGDFNS-----VSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNS 186

Query: 212 SWLKSKKFRLGARIVDEKNLGEFTRIGEAVSQPFRVKDHRGEVNKKHHPPRGEDEVWRLD 271
           SW +S+KFRLG RIVD+    ++ +I EA+++ F V+DHRGE+ KKHHPP   DEVWRL+
Sbjct: 187 SWTRSRKFRLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLE 246

Query: 272 GIGKNGVYHKNLSSNDIQTVDDFLNVHFEKGEVFLRKLLGKRVPDRVWNAMVKNASERFS 331
            IGK+G +H+ L+ ++I TV DFL  HF      LR++LG  +  ++W   + +A  R  
Sbjct: 247 KIGKDGAFHRRLNLSNINTVKDFL-THFHLNSSKLRQVLGTGMSSKMWEITLDHA--RSC 303

Query: 332 INDSNPNGFQ 337
           + DS+ + +Q
Sbjct: 307 VLDSSVHVYQ 303