Spg006452 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006452
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAP-3 complex subunit mu
Locationscaffold2: 49627320 .. 49631976 (+)
RNA-Seq ExpressionSpg006452
SyntenySpg006452
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAACATTTGCTCCTCTTTTACTATTCGATTTTGCAGAGAGATTATGCTGGAGAAACAGCTCACTGGGCATTGTGTTGACCGATCTATATGTGCTTGGTTCTGGGAGCAATCCCTATCTCAAGGTGATTCCTTCAAGGTACCTTTAGAATCGAAAATTTTCCATTCTTAATTGCTCTAGTTTTTGTGAATTCTAGGTTTTGCTCTCATATTTTGATATCTGGATCTTTCATTTTGAAGTATTTGTTCGAAGTTAACCTGTGGACTTATTTAGAACTTCGTTTTCTGATAATTCCATGGTGGTTTGATTTTGATTCTGTTTTTTCTTCCCATTTATTTTTAGCTGCAGCCGGTGATTGCTTCTCCAACACATTATCTTTTCCAAGTTGTTCGCGAGGGGATCACTTTCTTGGCCTGCACCCAAGTTGAAATGCCACCTTTAATGGGCATTGAGGTAAAGTTCTTACCTAGAATGAGCAAGAAGTTAGGTTGACTATTTGGGGTCCTTAACTTTTGATCCTTAGTTCCTCTGCAGAGTGGCTGATGTTCTCACAGATTATCTTGGAGAGTTGAATGAAGATTTGGTTAAGGATAATTTTGTCATTGTATATGAGGTATATCCATAGCTGTATACGCTTGCTGAGTTTGATTTAAGGTAGCACTTACTACTTTTAGTTCTAGAAATTTTCACAGGGAAAATGGATGGTTAAATTTATTCTCTTGATGTAAGTTCTCGATGTAGCATACTTGGTGTAGTTTTATAATTCATAATGTGAATTGTTAATGGTTTTAGTCTGTAGACTTTTTTTTCCCTTCTTTTTGACTTCAATATAGGGTGAAGGGATTCGAATCTTTGACCTTTAGGAAGGTGAGTACATTGACCAATTGAGTTATGCTCTATTTTTTTTTTATAAGAAACAATTTCATTGATGTATGAAACTACAGAAAAAGGGGGAGAGGAAAACCCTAAACCAAAGGAGTCACAAATAAACATCTCCAATTGGTCCAAAGAGAAGAAAGACTATAAGAGGAGAAGAGAGGGTTCAATTTACACCAAGATAAAGAAAAGAACACTATCAAAAAAGACCAGGTAAGGAGTTTCCTTTCCTTCAAAAGTCCTATGATTTCTTTTGATCAAAACCAACCACATGAAAGCCTGAACAATATTCTGTCAAAGGATATCCTTCTTTTCCTTAAAAGGGTGGCCCAAAAGGAGATTTAAAGAAGTGTATGGATTCTTTGGGAGAGTCAGCATCCAATTGAAAGATTTCATTCCAAAAGAGTTGTGCAAAATGGCTACAGATGAAAATGTGGGATTGAGACTCCATATCAAGTTTGCAAAGGATACACCTGCTTGGTGTGAGGGCCATGTAGGGAACTTTCTTTGGAGTCTGTCATGAGTGTTTAAAGCCCTGTGAGCAAGCTCCCAAACGAAGAATTTAATCTTCTTAGGGTAGTCATCGTGCCAAACAGCCTTCGGAAAAAAATATGGATTGTCCACAGTCGAGGTATCAAGGCTTTTGCGCAAAGATTTTGATGAGAAAGTCCCATTCTTCCTGGTTATTCAAGGGAATGCTGGATAAAGCAAAACTCAGTTCAGCCCATTCAATTATTTCATCATCCTTTAAATTCCTTATGAAATTCAAGCCCCGAAAAGCCAAGCCAAGGTTCCACAAGTCTTTTACAGAAGCTTTCTTTCTGTTTGATAAGGAGAAAAGTAGAGGGTAAGCAGAAGATAATGGAGAATTCCCTAGCCAGCAGTCATGCCAAAAGTCTATGGTTGCCCATTCCCAATTTTCGTCACCACAGAGTTATGAATTAAAGGCTGCTGTTTCAATATTGACTTCCAGGAACTGCGAGAGGAGTGTAGAGAGAAGCCTCCCGGGTGATTACTGAAGGCTTTTGTGCCAAATTTAGCATCAATAACCAGTCTCCAAAGAGCCGTCTTCTCCTTTTGATATCTCCACAGCCATTTTGCTAAAAGAGCACATTTTTTTGTACTTTATAGTGTGGAGGACCAAGCCATCTTTCTCAATAGGAAGAAGAATTTTTTCCCATTTAATAATATGTTTATGAAATCCCTTAACCTGTCCTCCCAAAGAAAGTTTCTGAGTAATCTCTCAATGTTCTTGACAATCTTTCTGGGCATTTCAAATAGGGAGATGTAATAGGTGGGGAGATTGGAAAGAGTTGCTTGGAGAAGCGTGTATCTCCCTCCTTTAGAAATACGGGTATTGTTCCAACAATTCAATCTTATTTGAATCTTTTCCACAATGGGGGACCAAAAAGAGGAAAGCTTGGAGTTATGGTCTATTGTAGTTTTTTTTTTAGTTTGTAGATAAGTAATTGTTTGTTTAGTTAGTGGGTTGTAGGTAGACTCATTAATCCTCCGTAAATAGAGGTTAAATTTTTTGTATTGATGGACTTCTTGAATCATAATAAAATACTCTTAAGAGAGTTCTTGGTGGTTTTCTCCTTTGATGCTTGGTCGCATCGGTTCTATGTGACTCAAACATGTCATGTAGATTATAAAATGCCAGTGAGAATAATGAATGAAGAGGGTTTTGTTCATATATTTCTAAGACACTTCTGTGTTTTTTTAATCATCTTTCATTTTTAAATTGATAATTTCATTTCACTCAACATATAAGTAATTGTTAGTCAATTGCAATTTGCAGCTTCTGGATGAGATGATAGACAATGGCTTCCCCCTTACTACAGAACCTAACATCCTGAGAGAAATCATAGCTCCACCAAACCTTGTCAGCAAAGTATTGAGTGTCGTGACTGGTAACAGTTCTAATGTGAGTGACACCCTTCCAGGCGCAATTGCATCTCATGTTCCATGGAGAACAACAGATCCAAAATATGCTAAAAATGAAGTTAATGTTGATCTGGTGGAGGAGATGGATGCGATTCTAAACAGGTCCTGAAATATTGCCTCTAGTTTTCATTTTTGTAATAGATATTAGAACTTATTCCATCCACCAATTATACTTTTGCCACTGTATACTTTGTTATGTTGAGAAAATTTAGTTTGGTCTCTGACATCATACAATATACAAAATTTTATGATCTTTCATAGATATGAATTTCACTACTGCATCTCCATATAAGTTTTAAATTTTAATGAATAACAAGCACATGAGAATGGTAGACATTTTTATTGAATCTGCTGTTGAATTGTGTTGTTTAGGGATGGCCACCTGATCAAGTGTGAAATTTATGGTCAGGTCCAAGTAAATTCTCATCTATCAGGTCTTCCTGATTTGACTCTTTCATTTACAAACCCTTCAATTCTTGATGATGTGAGATTCCATCCGTGTGTTCGGTTTCGCCCATGGGAATCACATCAAATCCTGTCCTTTGTGCCTCCTGATGGACAGTTTAAGCTCGTGAGTTACAGGTATATATAACTTAAAGAATATCTATACTATATTTTGGCTTCTTAAATTGAATTGCTTGTATCTGGAGTGGAGATGAGGGATATAGATACTTTCCAATATCTACTTTTCCAGTACTTTATGTGTTAATTATTAGCATTTCCTTATTTCAAAAAAAAAATAAAATAATAATAATAAATATTAGCATTTCCTCTTTACCGAATCAATTTTACTTCCGTTCAGGGTTAGAAAGTTGAAGAATACTCCTATATACATCAAGCCTCAGTTTACCTCAGATGCAGGAACATGTCGTGTCAGTGTGTTAGTAGGCATTCGACATGATCCTGGAAAGCCGATTGACTCGGTAGATGTGCAGTTTCAATTGCCCTCATGCGTTTTATCTGCTGATCTTACTTCAAACTATGGAACAGTGAACATCCTATCTAATAAGGTAATGGTTGCATCACTTTTTATTCCAAAAAACGTTATATTTCATTAAACAGAAGAAAACGTGTTGTATCCCTTCCCCACTTGCTAATCAACCAAAAGAGTTCTTCTAGATGCCTTTTTAAGTTGCTCTCAGGTTCATTGCAGTAACTTCTTAAATTTGTATATTGGCAATTTCTGATGTTTATATAATGAATCTGTAAGTTTCTTGATCGGGATTTCCTTATTGATTAGGCACCGAAAAGCTGTACAAATCTTTTTGCCTCCTGCCCACAGCTTACTGGCATTTCAAAAGGCTTAGATTCTACCATGCAAAAAGCATCTTCATCACTTTTTTCTCCCCCTCTTTTTTTACAAAAAAGCGTTTAGTACCTTCCAAATTTTTGCAGTTCATTTTCTATATTAGTGATCAGTGAAGGGAGTTGATTCCTGAATTGTTTGTTTGTTTGCTTGCTTAAGGATACTATATTCTATTCTTCTTATTCTCTTATAATATACCAAAATTTCAATGAAGTTCTATTTCTGTTGAGATTAAAAAAAATGGAATTCTTAAATTTCTTGTGAAATTTGTTTCTACAGATCTGCTCTTGGACAATTGGAAAGATGCCAAAAGATAAAACCCCTTCAATGTCTGGAACATTAACTCTTGAGACAGGTTTACAACAGCTTCATGTATTTCCAACATTTCAAGTGCGTTTTAAGATCATGGGAGTAGTCCTCTCTGGCCTGCAAGTAGATAAACTTGACGTCAAGAACTTGCCAAATAATCCTTACAAAGGCTTTCGAGCTCTCACACGAGCAGGGAAGTTCGAAGTTCGGTCATAA

mRNA sequence

ATGTTTAACATTTGCTCCTCTTTTACTATTCGATTTTGCAGAGAGATTATGCTGGAGAAACAGCTCACTGGGCATTGTGTTGACCGATCTATATGTGCTTGGTTCTGGGAGCAATCCCTATCTCAAGGTGATTCCTTCAAGCCGGTGATTGCTTCTCCAACACATTATCTTTTCCAAGTTGTTCGCGAGGGGATCACTTTCTTGGCCTGCACCCAAGTTGAAATGCCACCTTTAATGGGCATTGAGTTCCTCTGCAGAGTGGCTGATGTTCTCACAGATTATCTTGGAGAGTTGAATGAAGATTTGGTTAAGGATAATTTTGTCATTGTATATGAGGGAGATGTAATAGGTGGGGAGATTGGAAAGAGTTGCTTGGAGAAGCGTGTATCTCCCTCCTTTAGAAATACGGAACCTAACATCCTGAGAGAAATCATAGCTCCACCAAACCTTGTCAGCAAAGTATTGAGTGTCGTGACTGGTAACAGTTCTAATGTGAGTGACACCCTTCCAGGCGCAATTGCATCTCATGTTCCATGGAGAACAACAGATCCAAAATATGCTAAAAATGAAGTTAATGTTGATCTGGTGGAGGAGATGGATGCGATTCTAAACAGGGATGGCCACCTGATCAAGTGTGAAATTTATGGTCAGGTCCAAGTAAATTCTCATCTATCAGGTCTTCCTGATTTGACTCTTTCATTTACAAACCCTTCAATTCTTGATGATGTGAGATTCCATCCGTGTGTTCGGTTTCGCCCATGGGAATCACATCAAATCCTGTCCTTTGTGCCTCCTGATGGACAGTTTAAGCTCGTGAGTTACAGGGTTAGAAAGTTGAAGAATACTCCTATATACATCAAGCCTCAGTTTACCTCAGATGCAGGAACATGTCGTGTCAGTGTGTTAGTAGGCATTCGACATGATCCTGGAAAGCCGATTGACTCGGTAGATGTGCAGTTTCAATTGCCCTCATGCGTTTTATCTGCTGATCTTACTTCAAACTATGGAACAGTGAACATCCTATCTAATAAGATCTGCTCTTGGACAATTGGAAAGATGCCAAAAGATAAAACCCCTTCAATGTCTGGAACATTAACTCTTGAGACAGGTTTACAACAGCTTCATGTATTTCCAACATTTCAAGTGCGTTTTAAGATCATGGGAGTAGTCCTCTCTGGCCTGCAAGTAGATAAACTTGACGTCAAGAACTTGCCAAATAATCCTTACAAAGGCTTTCGAGCTCTCACACGAGCAGGGAAGTTCGAAGTTCGGTCATAA

Coding sequence (CDS)

ATGTTTAACATTTGCTCCTCTTTTACTATTCGATTTTGCAGAGAGATTATGCTGGAGAAACAGCTCACTGGGCATTGTGTTGACCGATCTATATGTGCTTGGTTCTGGGAGCAATCCCTATCTCAAGGTGATTCCTTCAAGCCGGTGATTGCTTCTCCAACACATTATCTTTTCCAAGTTGTTCGCGAGGGGATCACTTTCTTGGCCTGCACCCAAGTTGAAATGCCACCTTTAATGGGCATTGAGTTCCTCTGCAGAGTGGCTGATGTTCTCACAGATTATCTTGGAGAGTTGAATGAAGATTTGGTTAAGGATAATTTTGTCATTGTATATGAGGGAGATGTAATAGGTGGGGAGATTGGAAAGAGTTGCTTGGAGAAGCGTGTATCTCCCTCCTTTAGAAATACGGAACCTAACATCCTGAGAGAAATCATAGCTCCACCAAACCTTGTCAGCAAAGTATTGAGTGTCGTGACTGGTAACAGTTCTAATGTGAGTGACACCCTTCCAGGCGCAATTGCATCTCATGTTCCATGGAGAACAACAGATCCAAAATATGCTAAAAATGAAGTTAATGTTGATCTGGTGGAGGAGATGGATGCGATTCTAAACAGGGATGGCCACCTGATCAAGTGTGAAATTTATGGTCAGGTCCAAGTAAATTCTCATCTATCAGGTCTTCCTGATTTGACTCTTTCATTTACAAACCCTTCAATTCTTGATGATGTGAGATTCCATCCGTGTGTTCGGTTTCGCCCATGGGAATCACATCAAATCCTGTCCTTTGTGCCTCCTGATGGACAGTTTAAGCTCGTGAGTTACAGGGTTAGAAAGTTGAAGAATACTCCTATATACATCAAGCCTCAGTTTACCTCAGATGCAGGAACATGTCGTGTCAGTGTGTTAGTAGGCATTCGACATGATCCTGGAAAGCCGATTGACTCGGTAGATGTGCAGTTTCAATTGCCCTCATGCGTTTTATCTGCTGATCTTACTTCAAACTATGGAACAGTGAACATCCTATCTAATAAGATCTGCTCTTGGACAATTGGAAAGATGCCAAAAGATAAAACCCCTTCAATGTCTGGAACATTAACTCTTGAGACAGGTTTACAACAGCTTCATGTATTTCCAACATTTCAAGTGCGTTTTAAGATCATGGGAGTAGTCCTCTCTGGCCTGCAAGTAGATAAACTTGACGTCAAGAACTTGCCAAATAATCCTTACAAAGGCTTTCGAGCTCTCACACGAGCAGGGAAGTTCGAAGTTCGGTCATAA

Protein sequence

MFNICSSFTIRFCREIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSFRNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS
Homology
BLAST of Spg006452 vs. NCBI nr
Match: KAG6590499.1 (AP-3 complex subunit mu, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 775.8 bits (2002), Expect = 1.9e-220
Identity = 376/412 (91.26%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFKPVIASPTHYLFQVVREGITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           DLVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP
Sbjct: 193 DLVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAGTCRVSVLVGIRHDPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGTCRVSVLVGIRHDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           DS+DVQFQLPSCVLSADLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGLQQ
Sbjct: 313 DSIDVQFQLPSCVLSADLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LH+FPTFQVRFKIMGVVLS LQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 LHIFPTFQVRFKIMGVVLSALQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 413

BLAST of Spg006452 vs. NCBI nr
Match: XP_022961480.1 (AP-3 complex subunit mu isoform X2 [Cucurbita moschata])

HSP 1 Score: 775.4 bits (2001), Expect = 2.5e-220
Identity = 375/412 (91.02%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFKPVIASPTHYLFQVVREGITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           DLVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP
Sbjct: 193 DLVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAGTCRVSVLVGIRHDPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGTCRVSVLVGIRHDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           DS+DVQFQLPSC+LSADLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGLQQ
Sbjct: 313 DSIDVQFQLPSCILSADLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LH+FPTFQVRFKIMGVVLS LQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 LHIFPTFQVRFKIMGVVLSALQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 413

BLAST of Spg006452 vs. NCBI nr
Match: XP_023515690.1 (AP-3 complex subunit mu isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 772.3 bits (1993), Expect = 2.1e-219
Identity = 374/412 (90.78%), Postives = 390/412 (94.66%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFKPVIASPTHYLFQVVREGITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           DLVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP
Sbjct: 193 DLVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAG CRVSVLVGIRHDPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGMCRVSVLVGIRHDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           DS+DVQFQLPSCVLSADLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGLQQ
Sbjct: 313 DSIDVQFQLPSCVLSADLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LH+FPTFQVRFKIMG+VLS LQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 LHIFPTFQVRFKIMGLVLSALQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 413

BLAST of Spg006452 vs. NCBI nr
Match: XP_038894969.1 (AP-3 complex subunit mu isoform X2 [Benincasa hispida])

HSP 1 Score: 771.2 bits (1990), Expect = 4.8e-219
Identity = 376/412 (91.26%), Postives = 389/412 (94.42%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSIC WFW+QSLSQGDSFKPVIASPTHYLFQVVR GITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICTWFWDQSLSQGDSFKPVIASPTHYLFQVVRAGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           DLVEEMDAILNRDGHL+KCEIYG+VQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP
Sbjct: 193 DLVEEMDAILNRDGHLVKCEIYGEVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKLVSYRVRKLKNTP+Y+KPQFTSDAG CRVSVLVGIRHDPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLVSYRVRKLKNTPMYVKPQFTSDAGICRVSVLVGIRHDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           D++DVQFQLPSCVLSADLTSNYGTVNILS+KICSWTIGKMPKDKTPSMSGTLTLETGLQQ
Sbjct: 313 DAIDVQFQLPSCVLSADLTSNYGTVNILSSKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 413

BLAST of Spg006452 vs. NCBI nr
Match: KAG7024033.1 (AP-3 complex subunit mu [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.8 bits (1989), Expect = 6.2e-219
Identity = 376/414 (90.82%), Postives = 391/414 (94.44%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFK--PVIASPTHYLFQVVREGITFLACTQ 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFK  PVIASPTHYLFQVVREGITFLACTQ
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKLQPVIASPTHYLFQVVREGITFLACTQ 72

Query: 75  VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPS 134
           VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   
Sbjct: 73  VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNG 132

Query: 135 F-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEV 194
           F   TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEV
Sbjct: 133 FPLTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEV 192

Query: 195 NVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF 254
           NVDLVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF
Sbjct: 193 NVDLVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF 252

Query: 255 RPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGK 314
           RPWESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAGTCRVSVLVGIRHDPGK
Sbjct: 253 RPWESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGTCRVSVLVGIRHDPGK 312

Query: 315 PIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGL 374
           PIDS+DVQFQLPSCVLSADLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGL
Sbjct: 313 PIDSIDVQFQLPSCVLSADLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGL 372

Query: 375 QQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           QQLH+FPTFQVRFKIMGVVLS LQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 QQLHIFPTFQVRFKIMGVVLSALQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 415

BLAST of Spg006452 vs. ExPASy Swiss-Prot
Match: F4I562 (AP-3 complex subunit mu OS=Arabidopsis thaliana OX=3702 GN=AP3M PE=1 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 1.7e-182
Identity = 302/420 (71.90%), Postives = 359/420 (85.48%), Query Frame = 0

Query: 8   FTIRFCREIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFK--PVIASPTHYLFQVVREGI 67
           F I    E+MLEKQLTGH VDRSICAWFW+Q +SQGDSFK  PVIASPTHYLFQ+VR+GI
Sbjct: 6   FLISDSGEVMLEKQLTGHRVDRSICAWFWDQYISQGDSFKALPVIASPTHYLFQIVRDGI 65

Query: 68  TFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCL 127
           TFLAC+QVEMPPLM IEFLCRVADVL++YLG LNEDL+KDNF+IVYE            L
Sbjct: 66  TFLACSQVEMPPLMAIEFLCRVADVLSEYLGGLNEDLIKDNFIIVYE-----------LL 125

Query: 128 EKRVSPSF-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDP 187
           ++ +   F   TEP+IL+E+IAPPNLVSK+LSVVTGN+SNVSDTLP    S VPWR TDP
Sbjct: 126 DEMIDNGFPLTTEPSILKEMIAPPNLVSKMLSVVTGNASNVSDTLPSGAGSCVPWRPTDP 185

Query: 188 KYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVR 247
           KY+ NEV VDLVEEMDAI+NRDG L+KCEIYG+VQ+NS L+G PDLTLSF NPSIL+D+R
Sbjct: 186 KYSSNEVYVDLVEEMDAIVNRDGELVKCEIYGEVQMNSQLTGFPDLTLSFANPSILEDMR 245

Query: 248 FHPCVRFRPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVG 307
           FHPCVR+RPWESHQ+LSFVPPDG+FKL+SYRV+KLKNTP+Y+KPQ TSD+GTCR+SVLVG
Sbjct: 246 FHPCVRYRPWESHQVLSFVPPDGEFKLMSYRVKKLKNTPVYVKPQITSDSGTCRISVLVG 305

Query: 308 IRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGT 367
           IR DPGK I+S+ + FQLP CV SADL+SN+GTV ILSNK C+WTIG++PKDKTP +SGT
Sbjct: 306 IRSDPGKTIESITLSFQLPHCVSSADLSSNHGTVTILSNKTCTWTIGRIPKDKTPCLSGT 365

Query: 368 LTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVR 425
           L LE GL++LHVFPTF++ FKIMG+ LSGL+++KLD++ +P   YKGFRA TRAG+F+VR
Sbjct: 366 LALEPGLERLHVFPTFKLGFKIMGIALSGLRIEKLDLQTIPPRLYKGFRAQTRAGEFDVR 414

BLAST of Spg006452 vs. ExPASy Swiss-Prot
Match: P53677 (AP-3 complex subunit mu-2 OS=Homo sapiens OX=9606 GN=AP3M2 PE=1 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 5.2e-91
Identity = 183/430 (42.56%), Postives = 256/430 (59.53%), Query Frame = 0

Query: 4   ICSSFTIRFCREIMLEKQLTGHCVDRSICAWFWE--QSLSQGDSFKPVIASPTHYLFQVV 63
           I S F I    +I LEK      V RS+C +F+E  +  ++ ++  PVI +P HYL  V 
Sbjct: 2   IHSLFLINSSGDIFLEKHWKS-VVSRSVCDYFFEAQERATEAENVPPVIPTPHHYLLSVY 61

Query: 64  REGITFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIG 123
           R  I F+A  Q E+PPL  IEFL RV D   DY G  +E ++KDN V+VYE         
Sbjct: 62  RHKIFFVAVIQTEVPPLFVIEFLHRVVDTFQDYFGVCSEPVIKDNVVVVYE--------- 121

Query: 124 KSCLEKRVSPSF-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWR 183
              LE+ +   F   TE NIL+E+I PP ++  V++ +TG S+NV D LP    S VPWR
Sbjct: 122 --VLEEMLDNGFPLATESNILKELIKPPTILRTVVNTITG-STNVGDQLPTGQLSVVPWR 181

Query: 184 TTDPKYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSIL 243
            T  KY  NE   D++EE+DAI+++ G  I  EI G +     L+G+PDLTLSF NP +L
Sbjct: 182 RTGVKYTNNEAYFDVIEEIDAIIDKSGSTITAEIQGVIDACVKLTGMPDLTLSFMNPRLL 241

Query: 244 DDVRFHPCVRFRPWESHQILSFVPPDGQFKLVSYRV--RKLKNTPIYIKP--QFTSDAGT 303
           DDV FHPCVRF+ WES +ILSF+PPDG F+L+SY V  + L   P+Y+K    F   +  
Sbjct: 242 DDVSFHPCVRFKRWESERILSFIPPDGNFRLLSYHVSAQNLVAIPVYVKHNISFRDSSSL 301

Query: 304 CRVSVLVGIRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNI-LSNKICSWTIGKMPK 363
            R  + VG +   GK I+ V V  Q+P  VL+  LT + GT       K+ SW +GK+  
Sbjct: 302 GRFEITVGPKQTMGKTIEGVTVTSQMPKGVLNMSLTPSQGTHTFDPVTKMLSWDVGKINP 361

Query: 364 DKTPSMSGTLTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRAL 423
            K PS+ GT++L+ G  +    PT  ++FKI  + +SGL+V++LD+      P+KG + +
Sbjct: 362 QKLPSLKGTMSLQAGASKPDENPTINLQFKIQQLAISGLKVNRLDMYGEKYKPFKGIKYM 418

Query: 424 TRAGKFEVRS 426
           T+AGKF+VR+
Sbjct: 422 TKAGKFQVRT 418

BLAST of Spg006452 vs. ExPASy Swiss-Prot
Match: P53678 (AP-3 complex subunit mu-2 OS=Rattus norvegicus OX=10116 GN=Ap3m2 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.8e-91
Identity = 184/430 (42.79%), Postives = 258/430 (60.00%), Query Frame = 0

Query: 4   ICSSFTIRFCREIMLEKQLTGHCVDRSICAWFWE--QSLSQGDSFKPVIASPTHYLFQVV 63
           I S F I    +I LEK      V RS+C +F+E  +  ++ ++  PVI +P HYL  V 
Sbjct: 2   IHSLFLINSAGDIFLEKHWKS-VVSRSVCDYFFEAQERATEAENVPPVIPTPHHYLLSVY 61

Query: 64  REGITFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIG 123
           R  I F+A  Q E+PPL  IEFL RV D   DY G  +E ++KDN V+VYE         
Sbjct: 62  RHKIFFVAVIQTEVPPLFVIEFLHRVVDTFQDYFGVCSEPVIKDNVVVVYE--------- 121

Query: 124 KSCLEKRVSPSF-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWR 183
              LE+ +   F   TE NIL+E+I PP ++  V++ +TG S+NV D LP    S VPWR
Sbjct: 122 --VLEEMLDNGFPLATESNILKELIKPPTILRTVVNTITG-STNVGDQLPTGQLSVVPWR 181

Query: 184 TTDPKYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSIL 243
            T  KY  NE   D+VEE+DAI+++ G  +  EI G +     L+G+PDLTLSF NP +L
Sbjct: 182 RTGVKYTNNEAYFDVVEEIDAIIDKSGSTVTAEIQGVIDACVKLTGMPDLTLSFMNPRLL 241

Query: 244 DDVRFHPCVRFRPWESHQILSFVPPDGQFKLVSYRV--RKLKNTPIYIKPQFT-SDAGTC 303
           DDV FHPCVRF+ WES +ILSF+PPDG F+L+SY V  + L   P+Y+K   +  D+G+ 
Sbjct: 242 DDVSFHPCVRFKRWESERILSFIPPDGNFRLLSYHVSAQNLVAIPVYVKHSISFRDSGSL 301

Query: 304 -RVSVLVGIRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNI-LSNKICSWTIGKMPK 363
            R  + VG +   GK I+ V V  Q+P  VL+  LT + GT       K+ SW +GK+  
Sbjct: 302 GRFEITVGPKQTMGKTIEGVTVTSQMPKGVLNMSLTPSQGTHTFDPVTKMLSWDVGKINP 361

Query: 364 DKTPSMSGTLTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRAL 423
            K PS+ GT+ L+ G  +    PT  ++FKI  + +SGL+V++LD+      P+KG + +
Sbjct: 362 QKLPSLKGTMGLQVGASKPDENPTINLQFKIQQLAISGLKVNRLDMYGEKYKPFKGIKYM 418

Query: 424 TRAGKFEVRS 426
           T+AGKF+VR+
Sbjct: 422 TKAGKFQVRT 418

BLAST of Spg006452 vs. ExPASy Swiss-Prot
Match: Q9Y2T2 (AP-3 complex subunit mu-1 OS=Homo sapiens OX=9606 GN=AP3M1 PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 3.3e-90
Identity = 174/430 (40.47%), Postives = 260/430 (60.47%), Query Frame = 0

Query: 4   ICSSFTIRFCREIMLEKQLTGHCVDRSICAWFWE--QSLSQGDSFKPVIASPTHYLFQVV 63
           I S F I    +I LEK      V +S+C +F+E  +  +  ++  PVI++P HYL  + 
Sbjct: 2   IHSLFLINCSGDIFLEKHWKS-VVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIY 61

Query: 64  REGITFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIG 123
           R+ + F++  Q E+PPL  IEFL RVAD   DY GE +E  +KDN VIVYE         
Sbjct: 62  RDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYE--------- 121

Query: 124 KSCLEKRVSPSF-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWR 183
              LE+ +   F   TE NIL+E+I PP ++  V++ +TG SSNV DTLP    S++PWR
Sbjct: 122 --LLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITG-SSNVGDTLPTGQLSNIPWR 181

Query: 184 TTDPKYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSIL 243
               KY  NE   D+VEE+DAI+++ G  +  EI G +     LSG+PDL+LSF NP +L
Sbjct: 182 RAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLL 241

Query: 244 DDVRFHPCVRFRPWESHQILSFVPPDGQFKLVSYRV--RKLKNTPIYIKP--QFTSDAGT 303
           DDV FHPC+RF+ WES ++LSF+PPDG F+L+SYRV  + L   P+Y+K    F  ++  
Sbjct: 242 DDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSISFKENSSC 301

Query: 304 CRVSVLVGIRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNI-LSNKICSWTIGKMPK 363
            R  + +G + + GK I+ + V   +P  VL+ +LT   G+       K+ +W +GK+  
Sbjct: 302 GRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLTWDVGKITP 361

Query: 364 DKTPSMSGTLTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRAL 423
            K PS+ G + L++G  +    P+  ++FKI  + +SGL+V++LD+      P+KG + +
Sbjct: 362 QKLPSLKGLVNLQSGAPKPEENPSLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYV 418

Query: 424 TRAGKFEVRS 426
           T+AGKF+VR+
Sbjct: 422 TKAGKFQVRT 418

BLAST of Spg006452 vs. ExPASy Swiss-Prot
Match: Q5R478 (AP-3 complex subunit mu-1 OS=Pongo abelii OX=9601 GN=AP3M1 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 3.3e-90
Identity = 174/430 (40.47%), Postives = 260/430 (60.47%), Query Frame = 0

Query: 4   ICSSFTIRFCREIMLEKQLTGHCVDRSICAWFWE--QSLSQGDSFKPVIASPTHYLFQVV 63
           I S F I    +I LEK      V +S+C +F+E  +  +  ++  PVI++P HYL  + 
Sbjct: 2   IHSLFLINCSGDIFLEKHWKS-VVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIY 61

Query: 64  REGITFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIG 123
           R+ + F++  Q E+PPL  IEFL RVAD   DY GE +E  +KDN VIVYE         
Sbjct: 62  RDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYE--------- 121

Query: 124 KSCLEKRVSPSF-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWR 183
              LE+ +   F   TE NIL+E+I PP ++  V++ +TG SSNV DTLP    S++PWR
Sbjct: 122 --LLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITG-SSNVGDTLPTGQLSNIPWR 181

Query: 184 TTDPKYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSIL 243
               KY  NE   D+VEE+DAI+++ G  +  EI G +     LSG+PDL+LSF NP +L
Sbjct: 182 RAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLL 241

Query: 244 DDVRFHPCVRFRPWESHQILSFVPPDGQFKLVSYRV--RKLKNTPIYIKP--QFTSDAGT 303
           DDV FHPC+RF+ WES ++LSF+PPDG F+L+SYRV  + L   P+Y+K    F  ++  
Sbjct: 242 DDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSISFKENSSC 301

Query: 304 CRVSVLVGIRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNI-LSNKICSWTIGKMPK 363
            R  + +G + + GK I+ + V   +P  VL+ +LT   G+       K+ +W +GK+  
Sbjct: 302 GRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLTWDVGKITP 361

Query: 364 DKTPSMSGTLTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRAL 423
            K PS+ G + L++G  +    P+  ++FKI  + +SGL+V++LD+      P+KG + +
Sbjct: 362 QKLPSLKGLVNLQSGAPKPEENPSLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYV 418

Query: 424 TRAGKFEVRS 426
           T+AGKF+VR+
Sbjct: 422 TKAGKFQVRT 418

BLAST of Spg006452 vs. ExPASy TrEMBL
Match: A0A6J1HCC4 (AP-3 complex subunit mu isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462049 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.2e-220
Identity = 375/412 (91.02%), Postives = 391/412 (94.90%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFKPVIASPTHYLFQVVREGITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           DLVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP
Sbjct: 193 DLVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAGTCRVSVLVGIRHDPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGTCRVSVLVGIRHDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           DS+DVQFQLPSC+LSADLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGLQQ
Sbjct: 313 DSIDVQFQLPSCILSADLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LH+FPTFQVRFKIMGVVLS LQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 LHIFPTFQVRFKIMGVVLSALQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 413

BLAST of Spg006452 vs. ExPASy TrEMBL
Match: A0A6J1HVQ3 (AP-3 complex subunit mu isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467880 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 3.9e-219
Identity = 372/412 (90.29%), Postives = 390/412 (94.66%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFKPVIASPTHYLFQVVR+GITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKPVIASPTHYLFQVVRDGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWR+TDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRSTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           +LVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSF NPSILDDVRFHPCVRFRP
Sbjct: 193 ELVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFANPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAGTCRVSVLVGIRHDPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGTCRVSVLVGIRHDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           DS+DVQFQLPSCVLS DLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGLQQ
Sbjct: 313 DSIDVQFQLPSCVLSVDLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LH+FPTFQVRFKIMGVVLSGLQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 LHIFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 413

BLAST of Spg006452 vs. ExPASy TrEMBL
Match: A0A6J1HE62 (AP-3 complex subunit mu isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462049 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 3.9e-219
Identity = 375/414 (90.58%), Postives = 391/414 (94.44%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFK--PVIASPTHYLFQVVREGITFLACTQ 74
           EIMLEKQLTGH VDRSICAWFWEQS+SQGDSFK  PVIASPTHYLFQVVREGITFLACTQ
Sbjct: 13  EIMLEKQLTGHRVDRSICAWFWEQSVSQGDSFKLQPVIASPTHYLFQVVREGITFLACTQ 72

Query: 75  VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPS 134
           VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   
Sbjct: 73  VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNG 132

Query: 135 F-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEV 194
           F   TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEV
Sbjct: 133 FPLTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTIPGAIASHVPWRTTDPKYAKNEV 192

Query: 195 NVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF 254
           NVDLVEEMDAILNRDGHL+KCEIYG++QVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF
Sbjct: 193 NVDLVEEMDAILNRDGHLVKCEIYGEIQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF 252

Query: 255 RPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGK 314
           RPWESHQILSFVPPDGQFKL+SYRVRKLKNTP+Y+KPQFTSDAGTCRVSVLVGIRHDPGK
Sbjct: 253 RPWESHQILSFVPPDGQFKLMSYRVRKLKNTPVYVKPQFTSDAGTCRVSVLVGIRHDPGK 312

Query: 315 PIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGL 374
           PIDS+DVQFQLPSC+LSADLTSNYGTVNILSNK+CSWTIGKMPKDKTPSMSGTLTLETGL
Sbjct: 313 PIDSIDVQFQLPSCILSADLTSNYGTVNILSNKMCSWTIGKMPKDKTPSMSGTLTLETGL 372

Query: 375 QQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           QQLH+FPTFQVRFKIMGVVLS LQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 QQLHIFPTFQVRFKIMGVVLSALQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 415

BLAST of Spg006452 vs. ExPASy TrEMBL
Match: A0A1S3CL71 (AP-3 complex subunit mu isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502231 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.2e-219
Identity = 378/412 (91.75%), Postives = 388/412 (94.17%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFKPVIASPTHYLFQVVREGITFLACTQVE 74
           EIMLEKQLTGH VDRSIC WFWEQSLSQGDSFKPVIASPTHYLFQVVR GITFLACTQVE
Sbjct: 13  EIMLEKQLTGHRVDRSICVWFWEQSLSQGDSFKPVIASPTHYLFQVVRAGITFLACTQVE 72

Query: 75  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF- 134
           MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE            L++ +   F 
Sbjct: 73  MPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE-----------LLDEMIDNGFP 132

Query: 135 RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNV 194
             TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDT+PGAIASHVPWRTTDPKYAKNEVNV
Sbjct: 133 LTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTVPGAIASHVPWRTTDPKYAKNEVNV 192

Query: 195 DLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 254
           DLVEEMDAILNRDGHLIKCEIYG+VQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP
Sbjct: 193 DLVEEMDAILNRDGHLIKCEIYGEVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRFRP 252

Query: 255 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI 314
           WESHQILSFVPPDGQFKLVSYRVRKLKNTPIY+KPQFTSDAGTCRVSVLVGIR DPGKPI
Sbjct: 253 WESHQILSFVPPDGQFKLVSYRVRKLKNTPIYVKPQFTSDAGTCRVSVLVGIRRDPGKPI 312

Query: 315 DSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGLQQ 374
           DS+DVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLE+GLQQ
Sbjct: 313 DSIDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLESGLQQ 372

Query: 375 LHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           LHVFPT QVRFKIMGVVLSGLQVDKLDVKNLPN+PYKGFRALTRAG+FEVRS
Sbjct: 373 LHVFPTVQVRFKIMGVVLSGLQVDKLDVKNLPNHPYKGFRALTRAGQFEVRS 413

BLAST of Spg006452 vs. ExPASy TrEMBL
Match: A0A6J1DSB3 (AP-3 complex subunit mu OS=Momordica charantia OX=3673 GN=LOC111023846 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.0e-218
Identity = 375/414 (90.58%), Postives = 390/414 (94.20%), Query Frame = 0

Query: 15  EIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFK--PVIASPTHYLFQVVREGITFLACTQ 74
           EI+LEKQLTGH VDRSICAWFWEQSLSQGDSFK  PVIASPTHYLFQ+VREGITFLACTQ
Sbjct: 13  EIILEKQLTGHRVDRSICAWFWEQSLSQGDSFKLQPVIASPTHYLFQIVREGITFLACTQ 72

Query: 75  VEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPS 134
           VEMPPLMGIEFLCRVADVLTDYLGELNEDL+KDNFVI YE            L++ +   
Sbjct: 73  VEMPPLMGIEFLCRVADVLTDYLGELNEDLIKDNFVIAYE-----------LLDEMIDNG 132

Query: 135 F-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEV 194
           F   TEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEV
Sbjct: 133 FPLTTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEV 192

Query: 195 NVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF 254
           NVDLVEEMDAILNRDGHL+KCEIYG+VQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF
Sbjct: 193 NVDLVEEMDAILNRDGHLVKCEIYGEVQVNSHLSGLPDLTLSFTNPSILDDVRFHPCVRF 252

Query: 255 RPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGK 314
           RPWESHQILSFVPPDGQFKL+SYRVRKLKNTPIY+KPQFTSDAGTCR+SVLVG R+DPGK
Sbjct: 253 RPWESHQILSFVPPDGQFKLMSYRVRKLKNTPIYVKPQFTSDAGTCRISVLVGTRNDPGK 312

Query: 315 PIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGL 374
           PIDS+DVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGL
Sbjct: 313 PIDSIDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGTLTLETGL 372

Query: 375 QQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVRS 426
           QQLHVFPTFQVRFKIMG+VLSGLQVDKLDVKNLPN+PYKGFRALTRAGKFEVRS
Sbjct: 373 QQLHVFPTFQVRFKIMGIVLSGLQVDKLDVKNLPNHPYKGFRALTRAGKFEVRS 415

BLAST of Spg006452 vs. TAIR 10
Match: AT1G56590.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-183
Identity = 302/420 (71.90%), Postives = 359/420 (85.48%), Query Frame = 0

Query: 8   FTIRFCREIMLEKQLTGHCVDRSICAWFWEQSLSQGDSFK--PVIASPTHYLFQVVREGI 67
           F I    E+MLEKQLTGH VDRSICAWFW+Q +SQGDSFK  PVIASPTHYLFQ+VR+GI
Sbjct: 6   FLISDSGEVMLEKQLTGHRVDRSICAWFWDQYISQGDSFKALPVIASPTHYLFQIVRDGI 65

Query: 68  TFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCL 127
           TFLAC+QVEMPPLM IEFLCRVADVL++YLG LNEDL+KDNF+IVYE            L
Sbjct: 66  TFLACSQVEMPPLMAIEFLCRVADVLSEYLGGLNEDLIKDNFIIVYE-----------LL 125

Query: 128 EKRVSPSF-RNTEPNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDP 187
           ++ +   F   TEP+IL+E+IAPPNLVSK+LSVVTGN+SNVSDTLP    S VPWR TDP
Sbjct: 126 DEMIDNGFPLTTEPSILKEMIAPPNLVSKMLSVVTGNASNVSDTLPSGAGSCVPWRPTDP 185

Query: 188 KYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSILDDVR 247
           KY+ NEV VDLVEEMDAI+NRDG L+KCEIYG+VQ+NS L+G PDLTLSF NPSIL+D+R
Sbjct: 186 KYSSNEVYVDLVEEMDAIVNRDGELVKCEIYGEVQMNSQLTGFPDLTLSFANPSILEDMR 245

Query: 248 FHPCVRFRPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVG 307
           FHPCVR+RPWESHQ+LSFVPPDG+FKL+SYRV+KLKNTP+Y+KPQ TSD+GTCR+SVLVG
Sbjct: 246 FHPCVRYRPWESHQVLSFVPPDGEFKLMSYRVKKLKNTPVYVKPQITSDSGTCRISVLVG 305

Query: 308 IRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICSWTIGKMPKDKTPSMSGT 367
           IR DPGK I+S+ + FQLP CV SADL+SN+GTV ILSNK C+WTIG++PKDKTP +SGT
Sbjct: 306 IRSDPGKTIESITLSFQLPHCVSSADLSSNHGTVTILSNKTCTWTIGRIPKDKTPCLSGT 365

Query: 368 LTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGFRALTRAGKFEVR 425
           L LE GL++LHVFPTF++ FKIMG+ LSGL+++KLD++ +P   YKGFRA TRAG+F+VR
Sbjct: 366 LALEPGLERLHVFPTFKLGFKIMGIALSGLRIEKLDLQTIPPRLYKGFRAQTRAGEFDVR 414

BLAST of Spg006452 vs. TAIR 10
Match: AT1G60780.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 136.7 bits (343), Expect = 4.3e-32
Identity = 95/368 (25.82%), Postives = 179/368 (48.64%), Query Frame = 0

Query: 83  FLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF-RNTEPNIL 142
           FL RV DV   Y  EL E+ ++DNFV+VYE            L++ +   + + TE  IL
Sbjct: 86  FLHRVVDVFKHYFEELEEESLRDNFVVVYE-----------LLDEMMDFGYPQYTEARIL 145

Query: 143 REIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNVDLVEEMDA 202
            E I                   V+   P A+ + V WR+   +Y KNEV +D++E ++ 
Sbjct: 146 SEFIK-----------TDAYRMEVTQRPPMAVTNAVSWRSEGIQYKKNEVFLDVIENVNI 205

Query: 203 ILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSI--------------LDDVRFHP 262
           ++N +G +++ ++ G +++ ++L+G+P+  L   +  +              L+D++FH 
Sbjct: 206 LVNSNGQIVRSDVVGALKMRTYLTGMPECKLGLNDRVLLEAQGRATKGKAIDLEDIKFHQ 265

Query: 263 CVRFRPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRH 322
           CVR   +E+ + +SF+PPDG F L++YR+       I+++ Q  S + + RV +L+  R 
Sbjct: 266 CVRLARFENDRTISFIPPDGAFDLMTYRLSTQVKPLIWVEAQIESHSRS-RVEMLIKARS 325

Query: 323 D--PGKPIDSVDVQFQLPSCVLSADLTSNYGTVNILSNK-ICSWTIGKMPKDKTPSMSG- 382
                    +V+++  +P+   +  + ++ G+ +    K    W I   P +K   +   
Sbjct: 326 QFKERSTATNVEIELPVPTDASNPTVRTSLGSASYAPEKDALVWKIKSFPGNKEYMLRAE 385

Query: 383 ----TLTLETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDVKNLPNNPYKGF---RALT 425
               ++T E    +       +V+F+I    +SG+QV  L  K +  + Y+     R +T
Sbjct: 386 FHLPSITAEEATPERKA--PIRVKFEIPYFTVSGIQVRYL--KIIEKSGYQALPWVRYIT 426

BLAST of Spg006452 vs. TAIR 10
Match: AT4G24550.2 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 136.0 bits (341), Expect = 7.3e-32
Identity = 112/406 (27.59%), Postives = 185/406 (45.57%), Query Frame = 0

Query: 58  FQVVREGITFLACTQVEMPPLMGIEFLCRVADVLTDYLGELNEDLVKDNFVIVYE--GDV 117
           F V   G+ F+A T+V + P + +E L R+A V+ DYLG LNED  + NFV+VYE   +V
Sbjct: 60  FHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNFVLVYELLDEV 119

Query: 118 IG-GEIGKSCLEKRVSPSFRNTEPNILREIIAPPNLVSKV--LSVVTGNSSNVSDTLPGA 177
           I  G +  +  E  V  S+   EP     I+  P  +  +   ++ T  +      +PG 
Sbjct: 120 IDFGYVQTTSTE--VLKSYIFNEP-----IVVSPARLQPIDPAAIFTQGAKR----MPGT 179

Query: 178 IASHVPWRTTDPKYAKNEVNVDLVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTL 237
             +            + E+ VD++E++    +  G+++  EI G +Q+ S+LSG P++ L
Sbjct: 180 AVTKSVVANDPGGRRREEIFVDIIEKISVTFSSSGYILTSEIDGTIQMKSYLSGNPEIRL 239

Query: 238 SFT--------------------NPSILDDVRFHPCVRFRPWESHQILSFVPPDGQFKLV 297
           +                      +  ILDD  FH  VR   ++S + LS VPPDG+F ++
Sbjct: 240 ALNEDLNIGRGGRSVYDYRSSSGSGVILDDCNFHESVRLDSFDSDRTLSLVPPDGEFPVM 299

Query: 298 SYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGIRHDPGKPI--DSVDVQFQLPSCVLSAD 357
           +YR+ +    P ++      +AG  +  V++ IR +    I  +++ VQ  LP+    A 
Sbjct: 300 NYRMTQEFKPPFHVN-TLIEEAGRLKAEVIIKIRAEFPSDIIANTITVQMPLPNYTSRAS 359

Query: 358 LTSNYGTVNIL-----SNKICSWTIGKMPKDKTPSMSGTLTLETGLQQLHVFPT-----F 417
                G          SNK+  W + K+      ++   LT     Q+ H   T      
Sbjct: 360 FELEPGAAGQRTDFKESNKMLEWNLKKIVGGGEHTLRAKLTFS---QEFHGNITKEAGPV 419

Query: 418 QVRFKIMGVVLSGLQVDKLDV--KNLPNNPYKGFRALTRAGKFEVR 425
            + F I    +S LQV  L +  K+   NPY+  R +T+A  +  R
Sbjct: 420 SMTFTIPMYNVSKLQVKYLQIAKKSSSYNPYRWVRYVTQANSYVAR 450

BLAST of Spg006452 vs. TAIR 10
Match: AT1G10730.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 134.4 bits (337), Expect = 2.1e-31
Identity = 96/367 (26.16%), Postives = 175/367 (47.68%), Query Frame = 0

Query: 83  FLCRVADVLTDYLGELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVS---PSFRNTEPN 142
           FL RV DV   Y  EL E+ ++DNFV+VYE            L++ +    P F  TE  
Sbjct: 86  FLHRVVDVFKHYFEELEEESLRDNFVVVYE-----------LLDEMMDFGYPQF--TEAR 145

Query: 143 ILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGAIASHVPWRTTDPKYAKNEVNVDLVEEM 202
           IL E I                   V+   P A+ + V WR+   K+ KNEV +D++E +
Sbjct: 146 ILSEFIK-----------TDAYRMEVTQRPPMAVTNSVSWRSEGLKFKKNEVFLDVIESV 205

Query: 203 DAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSI--------------LDDVRF 262
           + ++N +G +++ ++ G +++ ++LSG+P+  L   +  +              L+D++F
Sbjct: 206 NILVNSNGQIVRSDVVGALKMRTYLSGMPECKLGLNDRILLEAQGRAIKGKAIDLEDIKF 265

Query: 263 HPCVRFRPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDAGTCRVSVLVGI 322
           H CVR   +E+ + +SF+PPDG F L++YR+       I+++      + + RV +LV  
Sbjct: 266 HQCVRLARFENDRTISFIPPDGSFDLMTYRLSTQVKPLIWVEAHIERHSRS-RVEMLVKA 325

Query: 323 RHD--PGKPIDSVDVQFQLPSCVLSADLTSNYGTVNILSNK-ICSWTIGKMPKDKTPSMS 382
           R          SV+++  +P+   + D+ ++ G+      K    W I     +K  ++ 
Sbjct: 326 RSQFKDRSYATSVEIELPVPTDAYNPDVRTSLGSAAYAPEKDALVWKIQYFYGNKEHTLK 385

Query: 383 GTLTLETGLQQLHVFP----TFQVRFKIMGVVLSGLQVDKLD-VKNLPNNPYKGFRALTR 425
               L + +      P      +V+F+I   ++SG+QV  L  ++      +   R +T 
Sbjct: 386 ADFHLPS-IAAEEATPERKAPIRVKFEIPKFIVSGIQVRYLKIIEKSGYQAHPWVRYITM 426

BLAST of Spg006452 vs. TAIR 10
Match: AT5G46630.1 (Clathrin adaptor complexes medium subunit family protein )

HSP 1 Score: 132.5 bits (332), Expect = 8.0e-31
Identity = 101/376 (26.86%), Postives = 170/376 (45.21%), Query Frame = 0

Query: 80  GIEFLCRVADVLTDYL-GELNEDLVKDNFVIVYEGDVIGGEIGKSCLEKRVSPSF-RNTE 139
           G +F+     +   Y  G  +ED +++NFV++YE            L++ +   + +N  
Sbjct: 81  GFKFVVEAVALFKSYFGGAFDEDAIRNNFVLIYE-----------LLDEIMDFGYPQNLS 140

Query: 140 PNILREIIAPPNLVSKVLSVVTGNSSNVSDTLPGA---IASHVPWRTTDPKYAKNEVNVD 199
           P IL+  I    + S        +S      +P A   +   V WR     Y KNEV +D
Sbjct: 141 PEILKLYITQEGVRSPF------SSKPKDKPVPNATLQVTGAVGWRREGLAYKKNEVFLD 200

Query: 200 LVEEMDAILNRDGHLIKCEIYGQVQVNSHLSGLPDLTLSFTNPSI--------------- 259
           +VE ++ +++  G++++C++ G+V +   LSG+PDL L   N  I               
Sbjct: 201 IVESVNLLMSSKGNVLRCDVTGKVLMKCFLSGMPDLKLGL-NDKIGLEKESEMKSRPAKS 260

Query: 260 -----LDDVRFHPCVRFRPWESHQILSFVPPDGQFKLVSYRVRKLKNTPIYIKPQFTSDA 319
                LDDV FH CV    + S + +SFVPPDG+F+L+ YR+ +  N P  + P    + 
Sbjct: 261 GKTIELDDVTFHQCVNLTRFNSEKTVSFVPPDGEFELMKYRITEGVNLPFRVLPTI-KEL 320

Query: 320 GTCRVSVLVGIRHDPGKPIDSVDVQFQLPSCVLSADLTSNYGTVNILSNKICS---WTIG 379
           G  R+ V V ++   G  + ++ V  ++P    +A       T     N       W I 
Sbjct: 321 GRTRMEVNVKVKSVFGAKMFALGVVVKIPVPKQTAKTNFQVTTGRAKYNPSIDCLVWKIR 380

Query: 380 KMPKDKTPSMSGTLTL--ETGLQQLHVFPTFQVRFKIMGVVLSGLQVDKLDV-KNLPNNP 425
           K P     ++S  + L    G ++    P  Q+ F++     SGL+V  L V +    N 
Sbjct: 381 KFPGQTESTLSAEIELISTMGEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKSGYNT 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6590499.11.9e-22091.26AP-3 complex subunit mu, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961480.12.5e-22091.02AP-3 complex subunit mu isoform X2 [Cucurbita moschata][more]
XP_023515690.12.1e-21990.78AP-3 complex subunit mu isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_038894969.14.8e-21991.26AP-3 complex subunit mu isoform X2 [Benincasa hispida][more]
KAG7024033.16.2e-21990.82AP-3 complex subunit mu [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
F4I5621.7e-18271.90AP-3 complex subunit mu OS=Arabidopsis thaliana OX=3702 GN=AP3M PE=1 SV=1[more]
P536775.2e-9142.56AP-3 complex subunit mu-2 OS=Homo sapiens OX=9606 GN=AP3M2 PE=1 SV=1[more]
P536788.8e-9142.79AP-3 complex subunit mu-2 OS=Rattus norvegicus OX=10116 GN=Ap3m2 PE=2 SV=1[more]
Q9Y2T23.3e-9040.47AP-3 complex subunit mu-1 OS=Homo sapiens OX=9606 GN=AP3M1 PE=1 SV=1[more]
Q5R4783.3e-9040.47AP-3 complex subunit mu-1 OS=Pongo abelii OX=9601 GN=AP3M1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HCC41.2e-22091.02AP-3 complex subunit mu isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462049... [more]
A0A6J1HVQ33.9e-21990.29AP-3 complex subunit mu isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467880 P... [more]
A0A6J1HE623.9e-21990.58AP-3 complex subunit mu isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462049... [more]
A0A1S3CL715.2e-21991.75AP-3 complex subunit mu isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502231 PE=3 ... [more]
A0A6J1DSB32.0e-21890.58AP-3 complex subunit mu OS=Momordica charantia OX=3673 GN=LOC111023846 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G56590.11.2e-18371.90Clathrin adaptor complexes medium subunit family protein [more]
AT1G60780.14.3e-3225.82Clathrin adaptor complexes medium subunit family protein [more]
AT4G24550.27.3e-3227.59Clathrin adaptor complexes medium subunit family protein [more]
AT1G10730.12.1e-3126.16Clathrin adaptor complexes medium subunit family protein [more]
AT5G46630.18.0e-3126.86Clathrin adaptor complexes medium subunit family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001392Clathrin adaptor, mu subunitPRINTSPR00314CLATHRINADPTcoord: 240..267
score: 50.65
coord: 347..358
score: 33.33
coord: 100..127
score: 31.49
IPR001392Clathrin adaptor, mu subunitPIRSFPIRSF005992AP_complex_mucoord: 4..425
e-value: 7.3E-85
score: 283.6
NoneNo IPR availableGENE3D2.60.40.1170Mu homology domain, subdomain Bcoord: 282..423
e-value: 3.8E-75
score: 254.6
NoneNo IPR availableGENE3D2.60.40.1170Mu homology domain, subdomain Bcoord: 177..420
e-value: 3.8E-75
score: 254.6
NoneNo IPR availableGENE3D3.30.450.60coord: 8..152
e-value: 7.0E-22
score: 79.8
NoneNo IPR availablePANTHERPTHR10529:SF340CARMINE, ISOFORM Acoord: 31..424
NoneNo IPR availablePANTHERPTHR10529AP COMPLEX SUBUNIT MUcoord: 31..424
NoneNo IPR availableCDDcd09252AP-3_Mu3_Ctermcoord: 177..424
e-value: 4.29656E-112
score: 327.621
NoneNo IPR availableCDDcd14837AP3_Mu_Ncoord: 15..152
e-value: 1.49607E-41
score: 141.888
IPR028565Mu homology domainPFAMPF00928Adap_comp_subcoord: 177..424
e-value: 3.2E-63
score: 213.6
IPR028565Mu homology domainPROSITEPS51072MHDcoord: 188..424
score: 30.89904
IPR036168AP-2 complex subunit mu, C-terminal superfamilySUPERFAMILY49447Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptorcoord: 177..424
IPR011012Longin-like domain superfamilySUPERFAMILY64356SNARE-likecoord: 15..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006452.1Spg006452.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006897 endocytosis
biological_process GO:0009630 gravitropism
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0030131 clathrin adaptor complex
cellular_component GO:0031410 cytoplasmic vesicle
cellular_component GO:0005794 Golgi apparatus