Spg006414 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006414
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionrhodanese-like domain-containing protein 6
Locationscaffold2: 49458715 .. 49463369 (+)
RNA-Seq ExpressionSpg006414
SyntenySpg006414
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAAAAACTTTGGGTTTTGGTCCTCTCTCAGCCCTCTTCCGGCGACGTCTTGGAGGCCCGTCCCGAACTCAATCCCTCGAAACTCGAAGCTCTCAGAGCTCTGAGCTCTGCCTCTCGCAATGGCGAATAACAAAGACGATGACAACCAATATGGCGTCCTGTTGTACTACAAGTACGCTCACATACCCGACCTTGACGACCTCGTTTCCTTCTACCAGTCCAATTGCTCCTCCCTCGCCCTTGTCGGCCGTGTCCGCCTCGCTTCCCAGGGCGTCAACGTCACTGTAAGCTTTTTTCAACTGCCTAATCTACCCCTTTCTCTATTGGGTTTCTAACAAATAAATTTGAGATGGGTTTTCTGTCAGTTCGAGCATTAGAATCAGCTGGAGTACTTGTGTATATGGGGTTTTCATTTGGCCTAATTTTGTCGGAACCCCATTTATCATTGATTTGCTCATTTCTATGCTCTGTGGTGATTCGGCGTTTTTCAATTTACTGCGGTGTTTTGAACATTCTCTCTTTCCCAATTAGGTTTGTGGAAAGTTATGCTCGTTGAAGAGTCATATTGCTGTATTGAAGTTGAATAGCTTGTTTGATGGGACTGATTTCAAGCTTGCATCGTGTCACCAACCCTTGAATGACATTGTAGCGAGAGAATGTGGATTCACTTCTCTGTCAGTTCGAGTTGTCCAAGTAAAAGATGTTCCTTCCTTTCAATTTTAAGCCCTTTTCTGCTGCAAAGTAATTTTGATGTTAAAACTTTCATATTTTCAGGAGTTGGTCACCCTCTGTTCAAATCCTCTATTGAAGTCACCTGAAATTACAGAAGCGGGAACCCATCTTTCTGCTGCCGAATTTCATTCTGTTCTTGAGAGTGCTGGTTAGATAATTCACTTGTTCTTCTGCTTTATTTGGTACTTTACTGACATAACAACATGGTTGACTTTATACAGAGCAGGGGCTGAAATGATTGGAAATGAAGTTCTTTTGACTAATCATCACATGCTTTGCAGGGCTACTTCCGGATGAAGAAACATCTGAACATATTAAAGATGTTGTTCTATTGGATGCAAGGAATTTATATGAGACTAGAGTTGGGAAGTTCCAAACACCAAACGTTGAAACTCTGGATCCTGAAATTAGGCAGTATAGTGATTTGCCATCTTGGATTGACGATAATTCTGAAAAGTTGCGAGGGAAACAAATTCTTATGTTAGTTTTCTTAAACTATAAGTTATTCAAATCCGAATGCCTTTTCCTTATTGTTCATGACATGACTCAAAATAATAGCACCAGTTGCTTATGTTTGGCATGCGGCTTACAGTGTAAATATCTGTTGATTCCTACTCATAGGAATGATGAGTACTTCGGGCATTTGGTTTAACACATATGTACCTTTGTCTGCATAATTGGATGTGAGTATGTGTTTGTGGTCAAGCCAAAGGTGCTAGATTTTTATTTTTAAGATCAGAACTACAATTTCGAGCTGCATATAATCAAATTTGGTTATTGCATCATTGATCATTGCATGCATTTGCAAATTCCAATTCCTAGGGCGTCTTACATGTGAATAGCTATTCTAGTTGGTCATTGTACAAAATGTCTTCCTTTGTATCAACCTTAGTTCATCAATGCTTGTGTAGTTGTGCATATAGACTACATTTGTAAATGGCTCATCTAGTAGCTCAAGTGGTAGGTCTTAGAATAGTACGCAATGACTTAAGTTTCATCCTCTTCCTATCTAATAGGTATTGCACTGGAGGAATCAGATGTGAGATGGCTTCAGCATATATTAAGTCAAAAGGATCGGGATTTCAAAATGTGTTTCAGGTGCAATCATATGAAAATTTCTTGATTTTATATCTTTTAAATACATAATGACATTAATTATATTGGATTTATGGACATATTTTCCCCAGTTTCGGACCTATCCATGTTAACTTATGAATTGATAGGCTAAAACTTTTTATCCAGTCTCTTAAGATTTAAGCTATGCGGTGGTGAGTCATGGTGTAAACATGCATTACGGCCGACATTTTTTAAGTAAACATTAGTTGGCTTTGGCTTTATCGCATCAGAGCCATTTTATATATACATATATATATTTTATGAATAGTCATTTTAAATTTTTATACATTGGTGATGGTGTGCTTTTCACTGCAGTTATATGGTGGAATACAACGTTATTTGGAACAATTTTCCGATGGTGGATTTTTTGCAGGGAAGAATTTTGTGTTTGATCACAGGTGAAATCTCTGATGCCCTCAGCATGGTAACAAGATTCTAAAGATGGAGACATCAGACAATATATATTCATTGAAATATTTAACCAAACAATTTTTTATGAACATAATTTGGCTCATCAAGTAAATTTCTTGAAACTTTTGAAGTCATCAAATATTCTGATAGGAATTGATGATTGCAACTTCATGGGAAACAGGGATAATGAATGATTTTGCAATTTGAGCTGAAATATTCTTAATATTCAATTCTTTGGTTATAGGATATCAGTTGGGAGCTCAACTGAAGATATTATAGGGACCTGCCTTGTTTGTAGAAACCCCTTTGATGATTATTCTTCTCGCTGTCGGTGTAAATATTGTCGAATGCTTGTTCTGGTGTGTGATAATTGCCAGGTCTGCCATAACATTAATCTCTTTGCACTCAATCTTATCAGGCTTGCTGTTCCATTATTTAGTGATTTCTAAGCATCAACTTGAATTCTAATATTTATTTTTCCTAACCCGTTATATCATTTCTGATCACCTTTAGCTTTCTTGTTATATGTGGTATCCTGCTTCTGCATCCTGTTATGCTCAGAAAAAGGAGACCCTATATGTTTGTGAGTTGTGTCGGAAGCATGGCAAGGATGGCAGGCAGATTGCACAATTCCAGAGCACAATTTCGGAAGTAGTCTCACTGCAAAATGAACTTGAAATTGTTTCCCAGCAACTTCCTTGGAGTAATGGTAAGGTTTGTACTAATTCTTCTATTATCCATTGCCTTAAGTTAATATCATAACAAGAATCAATTCCTTGATCTCAGAATGCTTCTAGAATAAGTGCTTTTTCCCCAATGTACTTTGACCTTACTGTTTACCAACAATTCACATTGTTGTTTGGTTTTACACTGGCACATTTAGATGTCCTTCTCGATTAAGAACTTGGGCCAAAAACCCTACCTGTGCTTTCTCATACAGCACTCTCAAGTGTTTTTTGACTTTTTACAAAAGTAATTTTACATTATTCCCCAACATGATGATTTAGAGGGCCAAAAGGATGTGATTATGATTGTAGCCACACAAATTCTAAGCCCCATCTATAATCCGATTGCTCTATATGAGCAATAAATTTTTATACTTCTTCAATGTGGTGTTATGTTCTCCATCAAATTATATTTCTGATGTTTAGAAACTTAATATGGACTATGGAGTTATTTCAAAAAAAAAAAAAAAAAAAAAAAAATATGGACTATGGAGTACTGGCTTGCCTTACTATTTTGAGCCTAAAAATTGGAAGCCAAATCTGTCTTCTACCTTTAATTGAGTTGTAATTAAAACTCTTATTCAGGTTGTAGGTCACCAAGAAAATTGAGAGTCCTATGTTTGCATGGATTTAGACAGAATGCCTCTAGTTTTAAGGGAAGAACTGCATCATTAGCAAAGAAACTCAAAACCATGGTGGAGTTTGTGTATGTCGATGCACCTCATGAGTTATCGTTCATTTACCATCCTCGGGACAGTGAGCCTCAGGAAACTTGTGTGAAATCTTCAGTACAACCAAATCGTCCTCCGCCATTAGAGTGTTGCAAGAAGAAGTTTGCTTGGTTAATAGCGGACAATGCAGGTGAAAGGAGTGAAACTAAATGGGAAGTTGCAGATGCCCCATTTGATCCACTCCAGTACCAGAAACAAACTGATGGGTTTGACGAATCATTGACATATTTGAAAACGGTATTTTCCGAGAAAGGACCATTCGATGGGATCTTGGGTTTTTCACAAGGAGCAGCAATGGCGGCCGCAGTTTGTTCAAGAAAAACGAGTCTAAAGGGTGTGATAGACTTTCGATTTGCAGTGCTGTGTTCTGGATTTCCACTTCAAATGGCAGAGTTAGACCACGGACTGATCAACTGCCCCTCACTTCACATATTTGGTAGTGATCGGGGAAACGATAGGCAGATCGCAAATGAAACGAGCAGAGACCTTGCTTCTAGTTTCGATCCAGGTTGTTCTGTGATAATTGAACATGACTCTGGTCACATAATTCCTACTCGATCTCCCTACATTGATGAGATAAAAGAGTTCCTTCAACGTTTTCTCTAGTTGGATTCATGAGTGTGACTTACAACCATTGAAGTTATTGCAGTAGGTATATGATCATTCAAGGGATTATCTTTGATTTCCCAAGAAAGGTTGGTTCACTCCTCCAGGGTTAGAATGGAACTTTTCTGCTTCATAGTTTTAGTTCATTGTAGACTTTTTTACCTTGTCAAATTTCTAGTTTACATACAATTTTTTTACATCAAAAGAATGAAGGATTTACAGAGTTTGGTGTAATAGTGGATCTTAAGTTAACAGGTTTCTGATTATTTAATCTTAAGTTACATAATTTCCTTTTGTAATTTCACTATTTCTAGTGT

mRNA sequence

ATGGCGAATAACAAAGACGATGACAACCAATATGGCGTCCTGTTGTACTACAAGTACGCTCACATACCCGACCTTGACGACCTCGTTTCCTTCTACCAGTCCAATTGCTCCTCCCTCGCCCTTGTCGGCCGTGTCCGCCTCGCTTCCCAGGGCGTCAACGTCACTGTTTGTGGAAAGTTATGCTCGTTGAAGAGTCATATTGCTGTATTGAAGTTGAATAGCTTGTTTGATGGGACTGATTTCAAGCTTGCATCGTGTCACCAACCCTTGAATGACATTGTAGCGAGAGAATGTGGATTCACTTCTCTGTCAGTTCGAGTTGTCCAAGAGTTGGTCACCCTCTGTTCAAATCCTCTATTGAAGTCACCTGAAATTACAGAAGCGGGAACCCATCTTTCTGCTGCCGAATTTCATTCTGTTCTTGAGAGTGCTGGGCTACTTCCGGATGAAGAAACATCTGAACATATTAAAGATGTTGTTCTATTGGATGCAAGGAATTTATATGAGACTAGAGTTGGGAAGTTCCAAACACCAAACGTTGAAACTCTGGATCCTGAAATTAGGCAGTATAGTGATTTGCCATCTTGGATTGACGATAATTCTGAAAAGTTGCGAGGGAAACAAATTCTTATGTATTGCACTGGAGGAATCAGATGTGAGATGGCTTCAGCATATATTAAGTCAAAAGGATCGGGATTTCAAAATGTGTTTCAGTTATATGGTGGAATACAACGTTATTTGGAACAATTTTCCGATGGTGGATTTTTTGCAGGGAAGAATTTTGTGTTTGATCACAGGATATCAGTTGGGAGCTCAACTGAAGATATTATAGGGACCTGCCTTGTTTGTAGAAACCCCTTTGATGATTATTCTTCTCGCTGTCGGTGTAAATATTGTCGAATGCTTGTTCTGGTGTGTGATAATTGCCAGGTCTGCCATAACATTAATCTCTTTGCACTCAATCTTATCAGGCTTGCTGTTCCATTATTTAGTGATTTCTAA

Coding sequence (CDS)

ATGGCGAATAACAAAGACGATGACAACCAATATGGCGTCCTGTTGTACTACAAGTACGCTCACATACCCGACCTTGACGACCTCGTTTCCTTCTACCAGTCCAATTGCTCCTCCCTCGCCCTTGTCGGCCGTGTCCGCCTCGCTTCCCAGGGCGTCAACGTCACTGTTTGTGGAAAGTTATGCTCGTTGAAGAGTCATATTGCTGTATTGAAGTTGAATAGCTTGTTTGATGGGACTGATTTCAAGCTTGCATCGTGTCACCAACCCTTGAATGACATTGTAGCGAGAGAATGTGGATTCACTTCTCTGTCAGTTCGAGTTGTCCAAGAGTTGGTCACCCTCTGTTCAAATCCTCTATTGAAGTCACCTGAAATTACAGAAGCGGGAACCCATCTTTCTGCTGCCGAATTTCATTCTGTTCTTGAGAGTGCTGGGCTACTTCCGGATGAAGAAACATCTGAACATATTAAAGATGTTGTTCTATTGGATGCAAGGAATTTATATGAGACTAGAGTTGGGAAGTTCCAAACACCAAACGTTGAAACTCTGGATCCTGAAATTAGGCAGTATAGTGATTTGCCATCTTGGATTGACGATAATTCTGAAAAGTTGCGAGGGAAACAAATTCTTATGTATTGCACTGGAGGAATCAGATGTGAGATGGCTTCAGCATATATTAAGTCAAAAGGATCGGGATTTCAAAATGTGTTTCAGTTATATGGTGGAATACAACGTTATTTGGAACAATTTTCCGATGGTGGATTTTTTGCAGGGAAGAATTTTGTGTTTGATCACAGGATATCAGTTGGGAGCTCAACTGAAGATATTATAGGGACCTGCCTTGTTTGTAGAAACCCCTTTGATGATTATTCTTCTCGCTGTCGGTGTAAATATTGTCGAATGCTTGTTCTGGTGTGTGATAATTGCCAGGTCTGCCATAACATTAATCTCTTTGCACTCAATCTTATCAGGCTTGCTGTTCCATTATTTAGTGATTTCTAA

Protein sequence

MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCRMLVLVCDNCQVCHNINLFALNLIRLAVPLFSDF
Homology
BLAST of Spg006414 vs. NCBI nr
Match: XP_038895686.1 (rhodanese-like domain-containing protein 6 [Benincasa hispida])

HSP 1 Score: 599.0 bits (1543), Expect = 2.5e-167
Identity = 294/324 (90.74%), Postives = 303/324 (93.52%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MANN+DDD+QYGVLLYYKYAHIPDLD LVSFYQSNC+SLALVGRVRLASQGVNVTVCGKL
Sbjct: 1   MANNEDDDHQYGVLLYYKYAHIPDLDHLVSFYQSNCTSLALVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
           CSLKSHIA +KLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPL 
Sbjct: 61  CSLKSHIAAMKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLS 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEIT+AGTHLSA EFHSVLESAGLL DEET E+ +DVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEITQAGTHLSATEFHSVLESAGLLSDEETLENSRDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDLPSWIDDNSEKLRGK ILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY
Sbjct: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKHILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGGFFAGKNFVFDHRISVGSST+DIIGTCL+C NPFDDYSSRCRCKYCR
Sbjct: 241 GGIQRYLEQFPDGGFFAGKNFVFDHRISVGSSTQDIIGTCLICGNPFDDYSSRCRCKYCR 300

Query: 301 MLVLVCDNCQVCHNINLFALNLIR 325
           MLVLVCDNCQ      LF   L R
Sbjct: 301 MLVLVCDNCQ--EKETLFVCELCR 322

BLAST of Spg006414 vs. NCBI nr
Match: XP_011654855.1 (rhodanese-like domain-containing protein 6 [Cucumis sativus] >KGN65012.1 hypothetical protein Csa_022714 [Cucumis sativus])

HSP 1 Score: 589.3 bits (1518), Expect = 2.0e-164
Identity = 286/310 (92.26%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MA N+DDD+QYGVLLYYKYA+IP+LDDLVSFYQSNC+SLALVGRVRLASQGVNVTVCGKL
Sbjct: 1   MAKNEDDDHQYGVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
            SLKSHIA +KLNSLFDGTDFKLASCHQP NDIVARECGFTSLSVRVVQELVTLCSNPL 
Sbjct: 61  SSLKSHIAAMKLNSLFDGTDFKLASCHQPSNDIVARECGFTSLSVRVVQELVTLCSNPLS 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEI+EAGTHLSAAEFHSVLE+AGLL DEET EH KDVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEISEAGTHLSAAEFHSVLETAGLLSDEETPEHNKDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
            TLDPEIRQYSDLPSWIDDNSEKL+GKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY
Sbjct: 181 VTLDPEIRQYSDLPSWIDDNSEKLQGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGGFFAGKNFVFDHRI+VGSST+DIIGTCL+C NPFDDYSSRCRCKYCR
Sbjct: 241 GGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTQDIIGTCLICGNPFDDYSSRCRCKYCR 300

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 301 MLVLVCDNCQ 310

BLAST of Spg006414 vs. NCBI nr
Match: XP_008464312.1 (PREDICTED: rhodanese-like domain-containing protein 6 [Cucumis melo])

HSP 1 Score: 588.2 bits (1515), Expect = 4.5e-164
Identity = 289/310 (93.23%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MANN+DDD  +GVLLYYKYA+IP+LDDLVSFYQSNC+SLALVGRVRLASQGVNVTVCGKL
Sbjct: 1   MANNEDDD--HGVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
            SLKSHIAV+KLNSLFDGTDFKLASCHQP NDIVARECGFTSLSVRVVQELVTLCSNPL 
Sbjct: 61  SSLKSHIAVMKLNSLFDGTDFKLASCHQPSNDIVARECGFTSLSVRVVQELVTLCSNPLS 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEI+EAGTHLSAAEFHSVLESAGLL DEET EH KDVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEISEAGTHLSAAEFHSVLESAGLLSDEETPEHNKDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY
Sbjct: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGGFFAGKNFVFDHRI+VGSST DIIGTCL+C NPFDDYSSRCRCKYCR
Sbjct: 241 GGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTPDIIGTCLICGNPFDDYSSRCRCKYCR 300

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 301 MLVLVCDNCQ 308

BLAST of Spg006414 vs. NCBI nr
Match: KAG7022387.1 (Rhodanese-like domain-containing protein 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 284/316 (89.87%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MAN +DDD+Q+GVLLYYKYA IPDLDDLVSFY+SNC SL LVGRVRLASQGVNVTVCGKL
Sbjct: 38  MANGEDDDHQFGVLLYYKYAQIPDLDDLVSFYKSNCISLGLVGRVRLASQGVNVTVCGKL 97

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
           CSLKSHIAV+KLNSLFDGTDFKLASCHQP+ND+VARECGFTSLSVRVVQELVTL SNPLL
Sbjct: 98  CSLKSHIAVMKLNSLFDGTDFKLASCHQPMNDVVARECGFTSLSVRVVQELVTLRSNPLL 157

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PE+TEAGTHLSAAEFHSVLESAGLL DE+   H KDVVLLDARNLYETRVGKF+TPNV
Sbjct: 158 KLPEVTEAGTHLSAAEFHSVLESAGLLSDEKKRIHSKDVVLLDARNLYETRVGKFKTPNV 217

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDL  WIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGS FQNVFQLY
Sbjct: 218 ETLDPEIRQYSDLSCWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSEFQNVFQLY 277

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF  GGFF+GKNFVFDHRISVGSSTEDIIGTCL C +PFDDYSSRCRCKYCR
Sbjct: 278 GGIQRYLEQFPHGGFFSGKNFVFDHRISVGSSTEDIIGTCLSCGDPFDDYSSRCRCKYCR 337

Query: 301 MLVLVCDNCQVCHNIN 317
           MLVLVCDNCQVC+N N
Sbjct: 338 MLVLVCDNCQVCYNSN 353

BLAST of Spg006414 vs. NCBI nr
Match: XP_022159722.1 (rhodanese-like domain-containing protein 6 [Momordica charantia])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 283/310 (91.29%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           M NN+DDD QYGVLLYYKYA IPDLDDLVSFY+SNCSSL LVGRVRLASQGVNVTVCGKL
Sbjct: 1   MVNNEDDD-QYGVLLYYKYAQIPDLDDLVSFYESNCSSLGLVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
           CSL+SHI  +KLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL
Sbjct: 61  CSLRSHITAMKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEITEAGTHLSAAEFHSVLESAGLL DEET  H KDVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEITEAGTHLSAAEFHSVLESAGLLSDEETPRHNKDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDLPSWIDDNSEKLR K+ILMYCTGGIRCEMASAYI+SKG  FQNVFQLY
Sbjct: 181 ETLDPEIRQYSDLPSWIDDNSEKLRAKKILMYCTGGIRCEMASAYIRSKGPDFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGG FAGKNFVFDHR+SVGSSTEDIIGTCL+C +PFDDYSSRCRCK CR
Sbjct: 241 GGIQRYLEQFPDGGLFAGKNFVFDHRMSVGSSTEDIIGTCLICGDPFDDYSSRCRCKNCR 300

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 301 MLVLVCDNCQ 309

BLAST of Spg006414 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.5e-130
Identity = 227/315 (72.06%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 6   DDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKS 65
           +D+ QYGVLLYYKY  +PDLD+LVSFY+S+C+SL L+GRVRL+ +GVNVTV GKL +L+ 
Sbjct: 15  EDEEQYGVLLYYKYTSVPDLDELVSFYESSCNSLGLLGRVRLSPKGVNVTVGGKLTALEE 74

Query: 66  HIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSPEI 125
           HIA  K N LF+GTDFKLASCH PLND VA ECGFTSLS+RVV+ELVT    P LK PEI
Sbjct: 75  HIAAAKSNCLFEGTDFKLASCHHPLNDKVAEECGFTSLSIRVVEELVTFSPCPPLKPPEI 134

Query: 126 TEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETLDP 185
           + AG HLSAAEFHSVL+SA        SE+ K++VLLDARNLYETR+GKF++ NVETLDP
Sbjct: 135 SNAGKHLSAAEFHSVLQSA-----NGKSEN-KELVLLDARNLYETRIGKFESENVETLDP 194

Query: 186 EIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQR 245
           EIRQYSDLP+WID N+EK++GK +LMYCTGGIRCEMASAYI+SKG+GF+N FQLYGGIQR
Sbjct: 195 EIRQYSDLPTWIDQNAEKMKGKNVLMYCTGGIRCEMASAYIRSKGAGFENTFQLYGGIQR 254

Query: 246 YLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCRMLVLV 305
           YLEQF  GGFF GKNFVFDHRISVGSS EDIIG+CL+C N FDDYS RCRC+ CRMLVLV
Sbjct: 255 YLEQFPSGGFFKGKNFVFDHRISVGSSKEDIIGSCLLCNNTFDDYSPRCRCRLCRMLVLV 314

Query: 306 CDNCQVCHNINLFAL 321
           C++C+V  +I +  L
Sbjct: 315 CNHCRVKGDIYICEL 323

BLAST of Spg006414 vs. ExPASy Swiss-Prot
Match: Q5T7W7 (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Homo sapiens OX=9606 GN=TSTD2 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-47
Identity = 117/298 (39.26%), Postives = 164/298 (55.03%), Query Frame = 0

Query: 13  VLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKSHIAVLKL 72
           VLLYY Y  + D   + ++  + C  L L G++R+A++G+N TV G   + + ++ V+  
Sbjct: 170 VLLYYCYHDLEDPQWICAWQTALCQHLHLTGKIRIAAEGINGTVGGSKLATRLYVEVMLS 229

Query: 73  NSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSPEITEAGTHL 132
             L     FK   C             F  L V V +E+V +  +P  K     + G HL
Sbjct: 230 FPL-----FKDDLCKDDFKTSKGGAHCFPELRVGVFEEIVPMGISP--KKISYKKPGIHL 289

Query: 133 SAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSD 192
           S  EFH  +E      ++E S    D +LLD RN YE+++G+FQ      L P+IR++S 
Sbjct: 290 SPGEFHKEVEKFLSQANQEQS----DTILLDCRNFYESKIGRFQ----GCLAPDIRKFSY 349

Query: 193 LPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFSD 252
            PS++D N E  R K++LMYCTGGIRCE  SAY+K+KG   + VFQL GGI +YLE+F D
Sbjct: 350 FPSYVDKNLELFREKRVLMYCTGGIRCERGSAYLKAKGV-CKEVFQLKGGIHKYLEEFPD 409

Query: 253 GGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCRMLVLVCDNCQ 311
            GF+ GK FVFD R ++ S   D++  C  C   +D Y   C    CR LVL C  CQ
Sbjct: 410 -GFYKGKLFVFDERYAL-SYNSDVVSECSYCGARWDQY-KLCSTPQCRQLVLTCPACQ 448

BLAST of Spg006414 vs. ExPASy Swiss-Prot
Match: Q5RCP1 (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Pongo abelii OX=9601 GN=TSTD2 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.7e-47
Identity = 116/298 (38.93%), Postives = 163/298 (54.70%), Query Frame = 0

Query: 13  VLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKSHIAVLKL 72
           VLLYY Y  + D   + ++  + C  L L G++R+A++G+N TV G   + + ++ V+  
Sbjct: 170 VLLYYCYRDLEDPQWICAWQTALCQQLHLTGKIRIAAEGINGTVGGSKLATRLYVEVMLS 229

Query: 73  NSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSPEITEAGTHL 132
             L     FK   C             F  L V V +E+V +  +P  K     + G HL
Sbjct: 230 FPL-----FKDDLCKDDFKTSKGGAHCFPELRVGVFEEIVPMGISP--KKISYKKPGIHL 289

Query: 133 SAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETLDPEIRQYSD 192
           S  EFH  +E      ++E S    D +LLD RN YE+++G+FQ      L P+IR++S 
Sbjct: 290 SPGEFHKEVEKFLSQANQEQS----DTILLDCRNFYESKIGRFQ----GCLAPDIRKFSY 349

Query: 193 LPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQFSD 252
            PS++D N E  R K++LMYCTGGIRCE  SAY+K+KG   + VFQL GGI +YLE+F D
Sbjct: 350 FPSYVDKNLELFREKRVLMYCTGGIRCERGSAYLKAKGV-CKEVFQLKGGIHKYLEEFPD 409

Query: 253 GGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCRMLVLVCDNCQ 311
            GF+ GK FVFD R ++ S   D++  C  C   +D Y   C    C  LVL C  CQ
Sbjct: 410 -GFYKGKLFVFDERYAL-SYNSDVVSECSYCGARWDQY-KLCSTPQCHQLVLTCPACQ 448

BLAST of Spg006414 vs. ExPASy Swiss-Prot
Match: Q3U269 (Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Mus musculus OX=10090 GN=Tstd2 PE=2 SV=3)

HSP 1 Score: 186.0 bits (471), Expect = 6.7e-46
Identity = 116/307 (37.79%), Postives = 171/307 (55.70%), Query Frame = 0

Query: 8   DNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKSHI 67
           D +  VLLYY Y  + D   + ++  + C  L L G++R+A++G+N TV G   + + ++
Sbjct: 164 DEEGEVLLYYCYCDLEDPHWVCAWQTALCHHLHLTGKIRIATEGINGTVGGSKVATRLYV 223

Query: 68  AVLKLNSLF----DGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSP 127
            V+    LF       DFK +             C F  L V V +E+V +  +P     
Sbjct: 224 EVMLSCPLFKDYLSEDDFKSSK--------GGSHC-FPELRVGVFEEIVPMGISP--SQV 283

Query: 128 EITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETL 187
              + G HLS  EFH  +E   LL   ++SE   + ++LD RN YE+++G+FQ      L
Sbjct: 284 SYKKPGIHLSPGEFHKEIEK--LL--SQSSEEQGNTIILDCRNFYESKIGRFQ----GCL 343

Query: 188 DPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGI 247
            P+IR++S  PS++D N +  R K++LMYCTGGIRCE  SAY+++KG   + VFQL GGI
Sbjct: 344 APDIRKFSYFPSYVDKNLDIFRQKRVLMYCTGGIRCERGSAYLRAKGV-CKEVFQLKGGI 403

Query: 248 QRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCRMLV 307
            +YLE+F D GF+ GK FVFD R ++  ++  ++  C  C  P+D Y   C    CR LV
Sbjct: 404 HKYLEEFPD-GFYKGKLFVFDERFALAYNS-SVVSECSYCGAPWDQY-KLCSTPQCRQLV 447

Query: 308 LVCDNCQ 311
           L C  CQ
Sbjct: 464 LTCSACQ 447

BLAST of Spg006414 vs. ExPASy Swiss-Prot
Match: Q65EW5 (tRNA uridine(34) hydroxylase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=trhO PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.8e-36
Identity = 99/285 (34.74%), Postives = 148/285 (51.93%), Query Frame = 0

Query: 9   NQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKSHIA 68
           NQY VLLYYKY HI + +     +   C  L L GR+ +A +G+N TV G +     +++
Sbjct: 3   NQYRVLLYYKYVHIDNPEQFAEDHLKFCKDLGLKGRILVAGEGINGTVSGTVEQTDRYMS 62

Query: 69  VLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSPEITEA 128
            +K +  F+   FK+           +    F  + VR   ELVTL     +   E+T  
Sbjct: 63  EMKSDPRFEDMVFKIDE---------SEGHAFKKMHVRHRDELVTLRLEDDIDPNELT-- 122

Query: 129 GTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETLDPEIR 188
           G +L   EF+  ++              +D +++DARN YE  +G F+      + P+I+
Sbjct: 123 GKYLEPKEFYEAMQQ-------------EDTIVVDARNDYEYDLGHFR----GAIRPDIK 182

Query: 189 QYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRY-L 248
            + +LP WI DN EKL GK+IL YCTGGIRCE  S ++K +  GF++V QL+GGI  Y  
Sbjct: 183 AFRELPEWIRDNKEKLEGKKILTYCTGGIRCEKFSGWLKKE--GFEDVSQLHGGIVTYGK 242

Query: 249 EQFSDGGFFAGKNFVFDHRISVGSSTED--IIGTCLVCRNPFDDY 291
           +    G  + GK +VFD RISV  + ++  I+G       P + Y
Sbjct: 243 DPEVQGELWDGKCYVFDERISVPVNQKEHVIVGKDYFTGEPCERY 257

BLAST of Spg006414 vs. ExPASy TrEMBL
Match: A0A0A0LW43 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G180760 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.8e-165
Identity = 286/310 (92.26%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MA N+DDD+QYGVLLYYKYA+IP+LDDLVSFYQSNC+SLALVGRVRLASQGVNVTVCGKL
Sbjct: 1   MAKNEDDDHQYGVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
            SLKSHIA +KLNSLFDGTDFKLASCHQP NDIVARECGFTSLSVRVVQELVTLCSNPL 
Sbjct: 61  SSLKSHIAAMKLNSLFDGTDFKLASCHQPSNDIVARECGFTSLSVRVVQELVTLCSNPLS 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEI+EAGTHLSAAEFHSVLE+AGLL DEET EH KDVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEISEAGTHLSAAEFHSVLETAGLLSDEETPEHNKDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
            TLDPEIRQYSDLPSWIDDNSEKL+GKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY
Sbjct: 181 VTLDPEIRQYSDLPSWIDDNSEKLQGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGGFFAGKNFVFDHRI+VGSST+DIIGTCL+C NPFDDYSSRCRCKYCR
Sbjct: 241 GGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTQDIIGTCLICGNPFDDYSSRCRCKYCR 300

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 301 MLVLVCDNCQ 310

BLAST of Spg006414 vs. ExPASy TrEMBL
Match: A0A1S3CLM0 (rhodanese-like domain-containing protein 6 OS=Cucumis melo OX=3656 GN=LOC103502226 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.2e-164
Identity = 289/310 (93.23%), Postives = 298/310 (96.13%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MANN+DDD  +GVLLYYKYA+IP+LDDLVSFYQSNC+SLALVGRVRLASQGVNVTVCGKL
Sbjct: 1   MANNEDDD--HGVLLYYKYANIPNLDDLVSFYQSNCTSLALVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
            SLKSHIAV+KLNSLFDGTDFKLASCHQP NDIVARECGFTSLSVRVVQELVTLCSNPL 
Sbjct: 61  SSLKSHIAVMKLNSLFDGTDFKLASCHQPSNDIVARECGFTSLSVRVVQELVTLCSNPLS 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEI+EAGTHLSAAEFHSVLESAGLL DEET EH KDVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEISEAGTHLSAAEFHSVLESAGLLSDEETPEHNKDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY
Sbjct: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGGFFAGKNFVFDHRI+VGSST DIIGTCL+C NPFDDYSSRCRCKYCR
Sbjct: 241 GGIQRYLEQFPDGGFFAGKNFVFDHRIAVGSSTPDIIGTCLICGNPFDDYSSRCRCKYCR 300

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 301 MLVLVCDNCQ 308

BLAST of Spg006414 vs. ExPASy TrEMBL
Match: A0A6J1E0L1 (rhodanese-like domain-containing protein 6 OS=Momordica charantia OX=3673 GN=LOC111026061 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.7e-162
Identity = 283/310 (91.29%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           M NN+DDD QYGVLLYYKYA IPDLDDLVSFY+SNCSSL LVGRVRLASQGVNVTVCGKL
Sbjct: 1   MVNNEDDD-QYGVLLYYKYAQIPDLDDLVSFYESNCSSLGLVGRVRLASQGVNVTVCGKL 60

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
           CSL+SHI  +KLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL
Sbjct: 61  CSLRSHITAMKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEITEAGTHLSAAEFHSVLESAGLL DEET  H KDVVLLDARNLYETRVGKFQTPNV
Sbjct: 121 KLPEITEAGTHLSAAEFHSVLESAGLLSDEETPRHNKDVVLLDARNLYETRVGKFQTPNV 180

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDLPSWIDDNSEKLR K+ILMYCTGGIRCEMASAYI+SKG  FQNVFQLY
Sbjct: 181 ETLDPEIRQYSDLPSWIDDNSEKLRAKKILMYCTGGIRCEMASAYIRSKGPDFQNVFQLY 240

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF DGG FAGKNFVFDHR+SVGSSTEDIIGTCL+C +PFDDYSSRCRCK CR
Sbjct: 241 GGIQRYLEQFPDGGLFAGKNFVFDHRMSVGSSTEDIIGTCLICGDPFDDYSSRCRCKNCR 300

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 301 MLVLVCDNCQ 309

BLAST of Spg006414 vs. ExPASy TrEMBL
Match: A0A6J1JM53 (rhodanese-like domain-containing protein 6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486437 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.1e-159
Identity = 277/310 (89.35%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MAN +DDD+Q+GVLLYYKYA IPDLDDLVSFY+SNC SL LVGRVRLASQGVNVTVCGKL
Sbjct: 31  MANGEDDDHQFGVLLYYKYAQIPDLDDLVSFYKSNCISLGLVGRVRLASQGVNVTVCGKL 90

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
           CSLKSHIAV+KLNSLFDGTDFKLASCHQP+ND+VARECGFTSLSVRVVQELVTL SNP++
Sbjct: 91  CSLKSHIAVMKLNSLFDGTDFKLASCHQPMNDVVARECGFTSLSVRVVQELVTLRSNPVM 150

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEITEAGTHLSAAEFHSVL SAGLL DE+   H KDVVLLDARNLYETRVGKF+TPN+
Sbjct: 151 KLPEITEAGTHLSAAEFHSVLGSAGLLSDEKKRIHSKDVVLLDARNLYETRVGKFKTPNI 210

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDL  WIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGS FQNVFQLY
Sbjct: 211 ETLDPEIRQYSDLSCWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSEFQNVFQLY 270

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF +GGFF+GKNFVFDHRISVGSSTEDIIGTCL C +PFDDYSSRCRCKYCR
Sbjct: 271 GGIQRYLEQFPNGGFFSGKNFVFDHRISVGSSTEDIIGTCLNCGDPFDDYSSRCRCKYCR 330

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 331 MLVLVCDNCQ 340

BLAST of Spg006414 vs. ExPASy TrEMBL
Match: A0A6J1JFL6 (rhodanese-like domain-containing protein 6 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486437 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.1e-159
Identity = 277/310 (89.35%), Postives = 292/310 (94.19%), Query Frame = 0

Query: 1   MANNKDDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKL 60
           MAN +DDD+Q+GVLLYYKYA IPDLDDLVSFY+SNC SL LVGRVRLASQGVNVTVCGKL
Sbjct: 31  MANGEDDDHQFGVLLYYKYAQIPDLDDLVSFYKSNCISLGLVGRVRLASQGVNVTVCGKL 90

Query: 61  CSLKSHIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLL 120
           CSLKSHIAV+KLNSLFDGTDFKLASCHQP+ND+VARECGFTSLSVRVVQELVTL SNP++
Sbjct: 91  CSLKSHIAVMKLNSLFDGTDFKLASCHQPMNDVVARECGFTSLSVRVVQELVTLRSNPVM 150

Query: 121 KSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNV 180
           K PEITEAGTHLSAAEFHSVL SAGLL DE+   H KDVVLLDARNLYETRVGKF+TPN+
Sbjct: 151 KLPEITEAGTHLSAAEFHSVLGSAGLLSDEKKRIHSKDVVLLDARNLYETRVGKFKTPNI 210

Query: 181 ETLDPEIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLY 240
           ETLDPEIRQYSDL  WIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGS FQNVFQLY
Sbjct: 211 ETLDPEIRQYSDLSCWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSEFQNVFQLY 270

Query: 241 GGIQRYLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCR 300
           GGIQRYLEQF +GGFF+GKNFVFDHRISVGSSTEDIIGTCL C +PFDDYSSRCRCKYCR
Sbjct: 271 GGIQRYLEQFPNGGFFSGKNFVFDHRISVGSSTEDIIGTCLNCGDPFDDYSSRCRCKYCR 330

Query: 301 MLVLVCDNCQ 311
           MLVLVCDNCQ
Sbjct: 331 MLVLVCDNCQ 340

BLAST of Spg006414 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 467.2 bits (1201), Expect = 1.1e-131
Identity = 227/315 (72.06%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 6   DDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKS 65
           +D+ QYGVLLYYKY  +PDLD+LVSFY+S+C+SL L+GRVRL+ +GVNVTV GKL +L+ 
Sbjct: 15  EDEEQYGVLLYYKYTSVPDLDELVSFYESSCNSLGLLGRVRLSPKGVNVTVGGKLTALEE 74

Query: 66  HIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVTLCSNPLLKSPEI 125
           HIA  K N LF+GTDFKLASCH PLND VA ECGFTSLS+RVV+ELVT    P LK PEI
Sbjct: 75  HIAAAKSNCLFEGTDFKLASCHHPLNDKVAEECGFTSLSIRVVEELVTFSPCPPLKPPEI 134

Query: 126 TEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPNVETLDP 185
           + AG HLSAAEFHSVL+SA        SE+ K++VLLDARNLYETR+GKF++ NVETLDP
Sbjct: 135 SNAGKHLSAAEFHSVLQSA-----NGKSEN-KELVLLDARNLYETRIGKFESENVETLDP 194

Query: 186 EIRQYSDLPSWIDDNSEKLRGKQILMYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQR 245
           EIRQYSDLP+WID N+EK++GK +LMYCTGGIRCEMASAYI+SKG+GF+N FQLYGGIQR
Sbjct: 195 EIRQYSDLPTWIDQNAEKMKGKNVLMYCTGGIRCEMASAYIRSKGAGFENTFQLYGGIQR 254

Query: 246 YLEQFSDGGFFAGKNFVFDHRISVGSSTEDIIGTCLVCRNPFDDYSSRCRCKYCRMLVLV 305
           YLEQF  GGFF GKNFVFDHRISVGSS EDIIG+CL+C N FDDYS RCRC+ CRMLVLV
Sbjct: 255 YLEQFPSGGFFKGKNFVFDHRISVGSSKEDIIGSCLLCNNTFDDYSPRCRCRLCRMLVLV 314

Query: 306 CDNCQVCHNINLFAL 321
           C++C+V  +I +  L
Sbjct: 315 CNHCRVKGDIYICEL 323

BLAST of Spg006414 vs. TAIR 10
Match: AT2G40760.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-24
Identity = 94/338 (27.81%), Postives = 151/338 (44.67%), Query Frame = 0

Query: 13  VLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKSHIAVLKL 72
           V+ +YK+A  P+  D     +  C  L + G + LA +G+N ++CG   S++  +A ++ 
Sbjct: 106 VVSFYKFADFPEHADFRKPLKDLCEKLRVSGGIILAPEGINGSICGIRESVEEVLAFIQR 165

Query: 73  NSLFDG----------TDFKLASCHQPLNDIVARECG---FTSLSVRVVQELVTLCSNPL 132
           +   +G              +   H   + + A E     +  + V++ +E+VTL    +
Sbjct: 166 DVRLNGLRQVETPVSPEQEAIHHGHSSSSPLAAGEDAPFRWDHVRVKLKKEIVTL---GI 225

Query: 133 LKSPEITEAGTHLSAAEFHSVLESAGLLPDEETSEHIKDVVLLDARNLYETRVGKFQTPN 192
                I   GT++S  E++       L+ D ET       V++D RN YETR+GKF+   
Sbjct: 226 PSVSPIERVGTYVSPEEWNE------LISDPET-------VVIDVRNTYETRIGKFK--- 285

Query: 193 VETLDPEIRQYSDLPSWI----------------------------DDNSEKLRG-KQIL 252
              +DP    + + PSW+                            +D +EK +   +I 
Sbjct: 286 -GAVDPCTTAFRNFPSWVENQFALKQEGNETQAKVEKEDFSEITHKEDKAEKPKTLPRIA 345

Query: 253 MYCTGGIRCEMASAYIKSKGSGFQNVFQLYGGIQRYLEQF-SDGGFFAGKNFVFDHRISV 300
           MYCTGGIRCE AS+ + S+  GF+ V+ L GGI +YLE+       + G+ FVFD R+SV
Sbjct: 346 MYCTGGIRCEKASSLLLSQ--GFEEVYHLKGGILKYLEEVPKTESLWEGECFVFDKRVSV 405

BLAST of Spg006414 vs. TAIR 10
Match: AT1G17850.2 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 8.2e-15
Identity = 74/279 (26.52%), Postives = 122/279 (43.73%), Query Frame = 0

Query: 6   DDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKS 65
           D+   + V+ +Y++  I D +  +  + S    L + GR+ L  QG+N    G      +
Sbjct: 78  DEGEDFIVVNFYRFVSIGDPEAEIEKHLSFLKDLNIRGRIYLNEQGINAQYSGPSKDALA 137

Query: 66  HIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVT----LCSNPLLK 125
           ++  LK +  F     +++               F  L ++    LV     +   PLL 
Sbjct: 138 YVEWLKGDDRFSDLLVQMSP--------AMNRHAFPKLKLQNKPSLVQYEGGISHLPLLD 197

Query: 126 SPEITEAGTHLSAAEFHSVLESAGLLPDEET--SEHIKDVVLLDARNLYETRVGKFQTPN 185
            P   +    L  +E+   L+   L  D+E   S   K  +LLD RN YE  VG F+  +
Sbjct: 198 PPMRAKP---LEPSEWKRKLKD--LTDDDEASPSNSGKSYILLDVRNGYEWDVGHFRGAH 257

Query: 186 VETLDPEIRQYSDLPSWIDDNSE---------KLRGKQILMYCTGGIRCEMASAYIKSKG 245
                PE+  + +    + D  E               ILMYCTGGIRC++ S  ++ + 
Sbjct: 258 ----RPEVDCFRNTSFGLSDEKEAPSDPLINVDKEKTDILMYCTGGIRCDVYSTVLRQR- 317

Query: 246 SGFQNVFQLYGGIQRYLEQFSDGGFFAGKNFVFDHRISV 270
            GF+N++ L GG+  YL++      + G  FVFD R+S+
Sbjct: 318 -GFKNLYTLKGGVSHYLKE-EGTAEWVGNLFVFDSRLSL 336

BLAST of Spg006414 vs. TAIR 10
Match: AT1G17850.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-14
Identity = 73/277 (26.35%), Postives = 121/277 (43.68%), Query Frame = 0

Query: 6   DDDNQYGVLLYYKYAHIPDLDDLVSFYQSNCSSLALVGRVRLASQGVNVTVCGKLCSLKS 65
           D+   + V+ +Y++  I D +  +  + S    L + GR+ L  QG+N    G      +
Sbjct: 78  DEGEDFIVVNFYRFVSIGDPEAEIEKHLSFLKDLNIRGRIYLNEQGINAQYSGPSKDALA 137

Query: 66  HIAVLKLNSLFDGTDFKLASCHQPLNDIVARECGFTSLSVRVVQELVT--LCSNPLLKSP 125
           ++  LK +  F     +++               F  L ++    L    +   PLL  P
Sbjct: 138 YVEWLKGDDRFSDLLVQMSP--------AMNRHAFPKLKLQNKPSLYEGGISHLPLLDPP 197

Query: 126 EITEAGTHLSAAEFHSVLESAGLLPDEET--SEHIKDVVLLDARNLYETRVGKFQTPNVE 185
              +    L  +E+   L+   L  D+E   S   K  +LLD RN YE  VG F+  +  
Sbjct: 198 MRAKP---LEPSEWKRKLKD--LTDDDEASPSNSGKSYILLDVRNGYEWDVGHFRGAH-- 257

Query: 186 TLDPEIRQYSDLPSWIDDNSE---------KLRGKQILMYCTGGIRCEMASAYIKSKGSG 245
              PE+  + +    + D  E               ILMYCTGGIRC++ S  ++ +  G
Sbjct: 258 --RPEVDCFRNTSFGLSDEKEAPSDPLINVDKEKTDILMYCTGGIRCDVYSTVLRQR--G 317

Query: 246 FQNVFQLYGGIQRYLEQFSDGGFFAGKNFVFDHRISV 270
           F+N++ L GG+  YL++      + G  FVFD R+S+
Sbjct: 318 FKNLYTLKGGVSHYLKE-EGTAEWVGNLFVFDSRLSL 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895686.12.5e-16790.74rhodanese-like domain-containing protein 6 [Benincasa hispida][more]
XP_011654855.12.0e-16492.26rhodanese-like domain-containing protein 6 [Cucumis sativus] >KGN65012.1 hypothe... [more]
XP_008464312.14.5e-16493.23PREDICTED: rhodanese-like domain-containing protein 6 [Cucumis melo][more]
KAG7022387.11.1e-16289.87Rhodanese-like domain-containing protein 6, partial [Cucurbita argyrosperma subs... [more]
XP_022159722.11.6e-16191.29rhodanese-like domain-containing protein 6 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q94AC11.5e-13072.06Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=ST... [more]
Q5T7W72.1e-4739.26Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Homo... [more]
Q5RCP14.7e-4738.93Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Pong... [more]
Q3U2696.7e-4637.79Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 OS=Mus ... [more]
Q65EW54.8e-3634.74tRNA uridine(34) hydroxylase OS=Bacillus licheniformis (strain ATCC 14580 / DSM ... [more]
Match NameE-valueIdentityDescription
A0A0A0LW439.8e-16592.26Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G180760 P... [more]
A0A1S3CLM02.2e-16493.23rhodanese-like domain-containing protein 6 OS=Cucumis melo OX=3656 GN=LOC1035022... [more]
A0A6J1E0L17.7e-16291.29rhodanese-like domain-containing protein 6 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1JM536.1e-15989.35rhodanese-like domain-containing protein 6 isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1JFL66.1e-15989.35rhodanese-like domain-containing protein 6 isoform X2 OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT1G09280.11.1e-13172.06CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas... [more]
AT2G40760.11.1e-2427.81Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT1G17850.28.2e-1526.52Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT1G17850.11.8e-1426.35Rhodanese/Cell cycle control phosphatase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001763Rhodanese-like domainSMARTSM00450rhod_4coord: 139..252
e-value: 5.2E-6
score: 36.0
IPR001763Rhodanese-like domainPROSITEPS50206RHODANESE_3coord: 155..251
score: 12.683699
IPR040503tRNA uridine(34) hydroxylase, N-terminalPFAMPF17773UPF0176_Ncoord: 12..110
e-value: 8.7E-17
score: 61.2
IPR022111Rhodanase, C-terminalPFAMPF12368Rhodanese_Ccoord: 258..310
e-value: 5.3E-13
score: 49.1
NoneNo IPR availableGENE3D3.30.70.100coord: 1..112
e-value: 4.7E-23
score: 83.3
NoneNo IPR availablePANTHERPTHR43268:SF3THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 1..310
NoneNo IPR availableCDDcd01518RHOD_YceAcoord: 129..248
e-value: 1.59116E-41
score: 138.096
IPR036873Rhodanese-like domain superfamilyGENE3D3.40.250.10coord: 121..282
e-value: 3.1E-40
score: 139.1
IPR036873Rhodanese-like domain superfamilySUPERFAMILY52821Rhodanese/Cell cycle control phosphatasecoord: 116..269
IPR020936tRNA uridine(34) hydroxylasePANTHERPTHR43268THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 1..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006414.1Spg006414.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding