Spg006338 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg006338 vs. NCBI nr
Match: XP_038888965.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1386.7 bits (3588), Expect = 0.0e+00
Identity = 689/773 (89.13%), Postives = 731/773 (94.57%), Query Frame = 0

Query: 1   MKQRTNSSAFLLL--LLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLR 60
           M Q T SSA  LL  LLLSSSL T TSAIKRSYVVYMGAHSHGGRKPAD+VADSHH+ LR
Sbjct: 1   MTQTTISSATFLLPFLLLSSSLLTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHKFLR 60

Query: 61  PFLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSW 120
           PFL+  DDF EDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVS+FLN+GRKLHTTRSW
Sbjct: 61  PFLKR-DDFTEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSIFLNKGRKLHTTRSW 120

Query: 121 KFMGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGIC 180
           +FMG+ENNNGVITSESIWKKARFGE TIIGNLDTGVWPESKSFS DDDLGPIP RW+GIC
Sbjct: 121 EFMGMENNNGVITSESIWKKARFGEDTIIGNLDTGVWPESKSFS-DDDLGPIPLRWRGIC 180

Query: 181 QNQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 240
           QNQ+DPSFHCNRKLIGARYF+KGYESVVG LNSSFNSPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYESVVGPLNSSFNSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGG 300
           SVFGLG GTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD AIHDGVDVLS+SLGG
Sbjct: 241 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDSAIHDGVDVLSVSLGG 300

Query: 301 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSG 360
           DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGP AG+VTNIAPWQITVGASTMDRKFPS 
Sbjct: 301 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPAAGTVTNIAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKI 420
           VVLGNKK IEGESLS  ALP++KFYPL+SAADVRLANAS +EAQLCKAGTLDPMKAKGKI
Sbjct: 361 VVLGNKKQIEGESLSQNALPSRKFYPLVSAADVRLANASAHEAQLCKAGTLDPMKAKGKI 420

Query: 421 LVCLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEY 480
           LVCLRGDNARVDKGEQALLAGA+GMILAN+E SGNEILADPHVLPASHIN+TDG  V  Y
Sbjct: 421 LVCLRGDNARVDKGEQALLAGAVGMILANNEPSGNEILADPHVLPASHINFTDGTAVLTY 480

Query: 481 INSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEA 540
           INSTK P AYITRATTQLGIRPAPFMAAFSSVGPNT+ PEILKPDVTAPG+SVIAAYTEA
Sbjct: 481 INSTKFPKAYITRATTQLGIRPAPFMAAFSSVGPNTIIPEILKPDVTAPGLSVIAAYTEA 540

Query: 541 EGPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDN 600
           EGPTNQE+D+RR+PFNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTA+TLDN
Sbjct: 541 EGPTNQEYDNRRLPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTANTLDN 600

Query: 601 NFEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFS 660
           NFEPLLNASYS ATP NYGAGH+HPNGATDPGL+YDIEV EYLLFLCALGY++AQISQFS
Sbjct: 601 NFEPLLNASYSVATPLNYGAGHVHPNGATDPGLIYDIEVNEYLLFLCALGYNQAQISQFS 660

Query: 661 DGPFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKP 720
            GPFNCS+PI+LTNLNYPSITVP+LSRSITITRR+KNVGSPGTYKA+IRKP+GISVWVKP
Sbjct: 661 SGPFNCSEPINLTNLNYPSITVPELSRSITITRRLKNVGSPGTYKAQIRKPAGISVWVKP 720

Query: 721 KKLKFTKVGEEQSFKVLLKVKKQNVA-RNYVFGDLIWSDGKHHVRSPIVVKAV 771
           KKL FT++GEEQSFKVL+KVK+QN A +NYV+GDLIWSDGKHHVRSPIVVK V
Sbjct: 721 KKLNFTRLGEEQSFKVLMKVKEQNAAKKNYVYGDLIWSDGKHHVRSPIVVKVV 771

BLAST of Spg006338 vs. NCBI nr
Match: TYK03381.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1377.1 bits (3563), Expect = 0.0e+00
Identity = 685/774 (88.50%), Postives = 726/774 (93.80%), Query Frame = 0

Query: 1   MKQRTNSSAFLLL---LLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELL 60
           MK  T SS+  LL   LLLSSSLFT TSAIKRSYVVYMGAHSHGGRKPAD+VADSHH+ L
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  RPFLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRS 120
           +PFL+SG++F +DVIFYSYTRHINGFAAMLEDEVAAQLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WKFMGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGI 180
           W+FMGLEN NGVI SESIWKKARFGE TIIGNLDTGVW ESKSFS DD++GPIP RWKGI
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFS-DDEMGPIPHRWKGI 180

Query: 181 CQNQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAG 240
           CQNQ+DPSFHCNRKLIGARYF+KGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAG
Sbjct: 181 CQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAG 240

Query: 241 ASVFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLG 300
           ASVFGLG+GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFD+AIHDGVDVLS+SLG
Sbjct: 241 ASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLG 300

Query: 301 GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS 360
           GDPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKFPS
Sbjct: 301 GDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPS 360

Query: 361 GVVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGK 420
            VVLGNKK IEGESLS EALP+KK YPLM+AADVRLANAS +EAQLCKAGTLDPMKAKGK
Sbjct: 361 LVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGK 420

Query: 421 ILVCLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFE 480
           ILVCLRGDNARVDKGEQALLAGA GMILAN+E SGNEILADPHVLPA HINYTDG  VF 
Sbjct: 421 ILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFA 480

Query: 481 YINSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTE 540
           YINSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNTVTPEILKPD+TAPG+SVIAAYTE
Sbjct: 481 YINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTE 540

Query: 541 AEGPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD 600
           AEGPTNQEFD+RRVPFNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD
Sbjct: 541 AEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD 600

Query: 601 NNFEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQF 660
           NNFEPLLNASY  ATPFNYGAGH+HPNGATDPGLVYDI+V EYL FLCALGYD+AQISQF
Sbjct: 601 NNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQF 660

Query: 661 SDGPFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVK 720
           SDGPFNCS+PISLTNLNYPSITVPKLSRSITITRR+KNVGSPGTY+A+IRKP+GISVWVK
Sbjct: 661 SDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVK 720

Query: 721 PKKLKFTKVGEEQSFKVLLKVKKQNVA-RNYVFGDLIWSDGKHHVRSPIVVKAV 771
           PKKL FTK+GEE SFKV +KVK   VA +NYV+GDLIWSDGKHHVRSPIVVK V
Sbjct: 721 PKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of Spg006338 vs. NCBI nr
Match: XP_008462019.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 683/768 (88.93%), Postives = 724/768 (94.27%), Query Frame = 0

Query: 6   NSSAFL--LLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLES 65
           +SS FL  L LLLSSSLFT TSAIKRSYVVYMGAHSHGGRKPAD+VADSHH+ L+PFL+S
Sbjct: 7   SSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFLQPFLKS 66

Query: 66  GDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGL 125
           G++F +DVIFYSYTRHINGFAAMLEDEVAAQLAKHP+VVSVFLNRGRKLHTTRSW+FMGL
Sbjct: 67  GEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGL 126

Query: 126 ENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQNQHD 185
           EN NGVI SESIWKKARFGE TIIGNLDTGVW ESKSFS DD++GPIP RWKGICQNQ+D
Sbjct: 127 ENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFS-DDEMGPIPHRWKGICQNQND 186

Query: 186 PSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGL 245
           PSFHCNRKLIGARYF+KGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGASVFGL
Sbjct: 187 PSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGL 246

Query: 246 GQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNPL 305
           G+GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFD+AIHDGVDVLS+SLGGDPNPL
Sbjct: 247 GKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGGDPNPL 306

Query: 306 FNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLGN 365
           FNDSVAIGSFHA+KHGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKFPS VVLGN
Sbjct: 307 FNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGN 366

Query: 366 KKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVCLR 425
           KK IEGESLS EALP+KK YPLM+AADVRLANAS +EAQLCKAGTLDPMKAKGKILVCLR
Sbjct: 367 KKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLR 426

Query: 426 GDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYINSTK 485
           GDNARVDKGEQALLAGA GMILAN+E SGNEILADPHVLPA HINYTDG  VF YINSTK
Sbjct: 427 GDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAYINSTK 486

Query: 486 LPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEGPTN 545
            P AYIT ATTQLGIRPAPFMAAFSSVGPNTVTPEILKPD+TAPG+SVIAAYTEAEGPTN
Sbjct: 487 FPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTN 546

Query: 546 QEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPL 605
           QEFD+RRVPFNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPL
Sbjct: 547 QEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPL 606

Query: 606 LNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDGPFN 665
           LNASY  ATPFNYGAGH+HPNGATDPGLVYDI+V EYL FLCALGYD+AQISQFSDGPFN
Sbjct: 607 LNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFSDGPFN 666

Query: 666 CSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKKLKF 725
           CS+PISLTNLNYPSITVPKLSRSITITRR+KNVGSPGTY+A+IRKP+GISVWVKPKKL F
Sbjct: 667 CSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKPKKLNF 726

Query: 726 TKVGEEQSFKVLLKVKKQNVA-RNYVFGDLIWSDGKHHVRSPIVVKAV 771
           TK+GEE SFKV +KVK   VA +NYV+GDLIWSDGKHHVRSPIVVK V
Sbjct: 727 TKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of Spg006338 vs. NCBI nr
Match: XP_023554401.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1369.4 bits (3543), Expect = 0.0e+00
Identity = 681/772 (88.21%), Postives = 729/772 (94.43%), Query Frame = 0

Query: 1   MKQRTNSSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPF 60
           MKQ T+ SA +LLLLLSS L T T+AIKRSYVVYMGAHSHGGRKPA+IVA+SH +LLRP+
Sbjct: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60

Query: 61  LESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKF 120
           LESG++FAEDVIFYSYTRHINGFAAMLEDEVAA+LA+HPKVVSVF NRGR+LHTTRSW+F
Sbjct: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120

Query: 121 MGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQN 180
           M LENN+GVI+SESIWKKARFGE TIIGNLDTGVWPESKSFSD DDLGPIP RW+GICQN
Sbjct: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180

Query: 181 QHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           Q+DPSFHCNRKLIGARYF+KGY+SVVG LNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 181 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240

Query: 241 FGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDP 300
           FGLG+G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVLS+SLGGDP
Sbjct: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVV 360
           NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS VV
Sbjct: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360

Query: 361 LGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILV 420
           LGNKK IEGESL+PE LP++KFYPLMSAADVRLANAS +EAQLCKAGTLDP KAKGKILV
Sbjct: 361 LGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420

Query: 421 CLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYIN 480
           CLRGDNARVDKGEQA+LAGA+GMILAN+E SGNEILADPHVLPASHIN+TDGV VF YIN
Sbjct: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480

Query: 481 STKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEG 540
           STK P AYIT ATTQLGIRPAPFMAAFSSVGP+T+TPEILKPDVTAPG+SVIAAYT AEG
Sbjct: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540

Query: 541 PTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 600
           PTNQE+D RRV FNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF
Sbjct: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 600

Query: 601 EPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDG 660
           +PLLNASYS ATPFNYGAGHIHPN ATDPGLVYDI V EYL FLCALGY++AQ+SQFS+G
Sbjct: 601 KPLLNASYSVATPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660

Query: 661 PFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKK 720
           PFNCS+PISLTNLNYPSITVP LSRSITITRR+KNVGSPGTYKA IRKP+GISV VKPKK
Sbjct: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720

Query: 721 LKFTKVGEEQSFKVLLKVKKQNVAR--NYVFGDLIWSDGKHHVRSPIVVKAV 771
           L FT++GEE SFKVL+KVK+ NV R  NYV+GDLIWSDGKHHVRSPIVV AV
Sbjct: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of Spg006338 vs. NCBI nr
Match: XP_022968976.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1367.8 bits (3539), Expect = 0.0e+00
Identity = 679/772 (87.95%), Postives = 726/772 (94.04%), Query Frame = 0

Query: 1   MKQRTNSSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPF 60
           MKQ T  S   LLLLLSS L T T+AIKRSYVVYMGAHSHGGRKPADIVADSH ELLRP+
Sbjct: 23  MKQPTYPSTTFLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADSHRELLRPY 82

Query: 61  LESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKF 120
           LESG++FAEDVIFYSYTRHINGFAAMLEDEVAA+LAKHP+VVSVF NRGR+LHTTRSW+F
Sbjct: 83  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRLHTTRSWEF 142

Query: 121 MGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQN 180
           M LENN+GVI+SESIWKKARFGE TIIGNLDTGVWPESKSFSD DDLGPIP RW+GICQN
Sbjct: 143 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 202

Query: 181 QHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           Q+DPSFHCNRKLIGARYF+KGY+SVVG LNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 203 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 262

Query: 241 FGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDP 300
           FGLG+G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVLS+SLGGDP
Sbjct: 263 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 322

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVV 360
           NPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS VV
Sbjct: 323 NPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 382

Query: 361 LGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILV 420
           LGNKK IEGESL+PE LP++KFYPLMSAADVRLANAS +EAQLCKAGTLDP KAKGKILV
Sbjct: 383 LGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 442

Query: 421 CLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYIN 480
           CLRGDNARVDKGEQA+LAGA+GMILAN+E SGNEILADPHVLPASHIN+TDGV VF YIN
Sbjct: 443 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 502

Query: 481 STKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEG 540
           STK P AYIT ATTQLG RPAPFMAAFSSVGP+T+TPEILKPDVTAPG+SVIAAYTEAEG
Sbjct: 503 STKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTEAEG 562

Query: 541 PTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 600
           PTNQE+D RRV FNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF
Sbjct: 563 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 622

Query: 601 EPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDG 660
           +PLLNASYS ATPFNYGAGHIHPN ATDPGLVYDI + EYL FLCALGY++AQ+SQFS+G
Sbjct: 623 KPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKAQLSQFSNG 682

Query: 661 PFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKK 720
           PFNCS+PISLTNLNYPSITVP LSRSITITRR+KNVGSPGTYKA IR+P+GISV VKPKK
Sbjct: 683 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGISVSVKPKK 742

Query: 721 LKFTKVGEEQSFKVLLKVKKQNVAR--NYVFGDLIWSDGKHHVRSPIVVKAV 771
           L FT++GEE SFKVL+KVK+ NV R  NYV+GDLIWSDGKHHVRSPIVV A+
Sbjct: 743 LSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of Spg006338 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 3.8e-286
Identity = 493/771 (63.94%), Postives = 600/771 (77.82%), Query Frame = 0

Query: 5   TNSSAFLLLLLL--SSSLFTLTSAIKRSYVVYMGAHSHGG---RKPADIVADSHHELLRP 64
           T++ +FLLLLLL   SS   L S    SYVVY GAHSH G       D V ++H++ L  
Sbjct: 4   THNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGS 63

Query: 65  FLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWK 124
           F  S +  A D IFYSYT+HINGFAA L+ ++A +++KHP+VVSVF N+  KLHTTRSW 
Sbjct: 64  FTGSRER-ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWD 123

Query: 125 FMGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQ 184
           F+GLE+N+  + S SIW+KARFGE TII NLDTGVWPESKSF  D+ LGPIP RWKGICQ
Sbjct: 124 FLGLEHNS-YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFR-DEGLGPIPSRWKGICQ 183

Query: 185 NQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGAS 244
           NQ D +FHCNRKLIGARYF+KGY + VG LNSSF+SPRD +GHGSHTLSTA G+FV G S
Sbjct: 184 NQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVS 243

Query: 245 VFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGD 304
           +FG G GTAKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+S+SLGG+
Sbjct: 244 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 303

Query: 305 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGV 364
           P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +V+N+APWQITVGASTMDR+F S +
Sbjct: 304 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 363

Query: 365 VLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKIL 424
           VLGN K  +G+SLS  ALP+ KFYP+M++ + +  NAS  +AQLCK G+LDP+K KGKIL
Sbjct: 364 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 423

Query: 425 VCLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYI 484
           VCLRG N RV+KG    L G +GM+L N+  +GN++LADPHVLPA+ +   D   V  YI
Sbjct: 424 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 483

Query: 485 NSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAE 544
           + TK P+A+IT + T LG++PAP MA+FSS GP+ V P+ILKPD+TAPGVSVIAAYT A 
Sbjct: 484 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 543

Query: 545 GPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNN 604
            PTN++FD RR+ FN+ISGTSMSCPH+SGIAGLL+T YP WSPAAI+SAIMTTA+ +D+ 
Sbjct: 544 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 603

Query: 605 FEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSD 664
             P+ NA+  KATPF++GAGH+ PN A +PGLVYD+ +K+YL FLC+LGY+ +QIS FS 
Sbjct: 604 PGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG 663

Query: 665 GPFNCSKP-ISLTNLNYPSITVPKLSRS-ITITRRVKNVGSPGTYKARIRKPSGISVWVK 724
             F CS P ISL NLNYPSITVP L+ S +T++R VKNVG P  Y  ++  P G+ V VK
Sbjct: 664 NNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVK 723

Query: 725 PKKLKFTKVGEEQSFKVLLKVKKQNVARNYVFGDLIWSDGKHHVRSPIVVK 769
           P  L FTKVGE+++FKV+L   K NVA+ YVFG+L+WSD KH VRSPIVVK
Sbjct: 724 PTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

BLAST of Spg006338 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 2.6e-258
Identity = 451/761 (59.26%), Postives = 572/761 (75.16%), Query Frame = 0

Query: 13  LLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPA---DIVADSHHELLRPFLESGDDFAE 72
           LLLL +  F+   A+K+SY+VY+G+H+H  +  +   D VA SH   L  F+ S ++ A+
Sbjct: 24  LLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN-AK 83

Query: 73  DVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGLENNNGV 132
           + IFYSY RHINGFAA+L++  AA++AKHP VVSVF N+GRKLHTT SW FM L   NGV
Sbjct: 84  EAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM-LLAKNGV 143

Query: 133 ITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQNQHDPSFHCN 192
           +   S+W KA +GE TII NLDTGVWPESKSFS D+  G +P RWKG C         CN
Sbjct: 144 VHKSSLWNKAGYGEDTIIANLDTGVWPESKSFS-DEGYGAVPARWKGRCHK----DVPCN 203

Query: 193 RKLIGARYFHKGYESVVG-RLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGQGTA 252
           RKLIGARYF+KGY +  G   N+S+ + RD++GHGSHTLSTA GNFV GA+VFG+G GTA
Sbjct: 204 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 263

Query: 253 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNPLFNDSV 312
            GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DGVDVLS S+GGD     +D +
Sbjct: 264 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 323

Query: 313 AIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLGNKKWIE 372
           AIGSFHAVK+G+ V+CSAGNSGP +G+V+N+APW ITVGAS+MDR+F + V L N +  +
Sbjct: 324 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 383

Query: 373 GESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVCLRGDNAR 432
           G SLS + LP +K Y L+SAAD  +AN +V +A LCK G+LDP K KGKILVCLRGDNAR
Sbjct: 384 GTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 443

Query: 433 VDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYINSTKLPVAY 492
           VDKG QA  AGA GM+L N +ASGNEI++D HVLPAS I+Y DG T+F Y++STK P  Y
Sbjct: 444 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 503

Query: 493 ITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEGPTNQEFDH 552
           I   T  L  +PAPFMA+FSS GPNT+TP ILKPD+TAPGV++IAA+TEA GPT+ + D+
Sbjct: 504 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 563

Query: 553 RRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY 612
           RR PFN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAI+SAIMTT+ T +N  +P+++ S+
Sbjct: 564 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 623

Query: 613 SKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDGP-FNCSKP 672
            KA PF+YG+GH+ PN A  PGLVYD+   +YL FLCA+GY+   +  F++ P + C + 
Sbjct: 624 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 683

Query: 673 ISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKKLKFTKVG 732
            +L + NYPSITVP L+ SIT+TR++KNVG P TY AR R+P G+ V V+PK+L F K G
Sbjct: 684 ANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 743

Query: 733 EEQSFKVLLKVKKQNVARNYVFGDLIWSDGKHHVRSPIVVK 769
           E + F++ L+      +  YVFG+L W+D  H+VRSPIVV+
Sbjct: 744 EVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQ 775

BLAST of Spg006338 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 711.1 bits (1834), Expect = 1.4e-203
Identity = 400/793 (50.44%), Postives = 520/793 (65.57%), Query Frame = 0

Query: 7   SSAFLLLLLLSS-SLFT-LTSAI---KRSYVVYMGAHSHGGRKPA---DIVADSHHELLR 66
           SS F L L+LSS  LFT L +A+   K+ Y+VYMGAHSHG    +   ++  DSH++LL 
Sbjct: 3   SSIFCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLG 62

Query: 67  PFLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSW 126
               S +  A++ I YSY RHINGFAA+LE+E AA +AK+P VVSVFL++  KLHTTRSW
Sbjct: 63  SIFGSREK-AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSW 122

Query: 127 KFMGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWK-GI 186
           +F+GL          S W+K RFGE TIIGN+DTGVWPES+SFS D   G +P +W+ G+
Sbjct: 123 EFLGLHRRG----QNSAWQKGRFGENTIIGNIDTGVWPESQSFS-DKGYGTVPSKWRGGL 182

Query: 187 CQNQHDPSF---HCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNF 246
           CQ    P      CNRKLIGARY++K +E+  G+L+   ++ RD  GHG+HTLSTAGGNF
Sbjct: 183 CQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNF 242

Query: 247 VAGASVFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSI 306
           V GA VF +G GTAKGGSPRARVAAYKVCW       C+ AD+LAA D AI DGVDV+++
Sbjct: 243 VPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINV 302

Query: 307 SLGGD----PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGAST 366
           S G         +F D ++IG+FHA+   I+++ SAGN GPT G+V N+APW  T+ AST
Sbjct: 303 SFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAAST 362

Query: 367 MDRKFPSGVVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLD 426
           +DR F S + + N + IEG SL     PN+ F  L+ + D +LANA+  +AQLC+ GTLD
Sbjct: 363 LDRDFSSNLTI-NNQLIEGASLFVNLPPNQAF-SLILSTDAKLANATFRDAQLCRRGTLD 422

Query: 427 PMKAKGKILVCLR-GDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINY 486
             K  GKI++C R G    V +G +AL AGA GMIL N   +G  + A+PHV    +   
Sbjct: 423 RTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPP 482

Query: 487 TDGVTVFEYINSTKL----------PVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEI 546
               +    + +T +              ++RA T  G +PAP MA+FSS GPN + P I
Sbjct: 483 RRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSI 542

Query: 547 LKPDVTAPGVSVIAAYTEAEGPTNQEFDHRR-VPFNSISGTSMSCPHVSGIAGLLRTLYP 606
           LKPDVTAPGV+++AAY+E    ++   D+RR   FN + GTSMSCPH SGIAGLL+T +P
Sbjct: 543 LKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHP 602

Query: 607 HWSPAAIKSAIMTTASTLDNNFEPLLNA-SYSKATPFNYGAGHIHPNGATDPGLVYDIEV 666
            WSPAAIKSAIMTTA+TLDN   P+ +A   + A  F YG+GH+ P+ A +PGLVYD+ +
Sbjct: 603 SWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSL 662

Query: 667 KEYLLFLCALGYDEAQISQFS-DGPFNCSKPISLTNLNYPSITVPKLS-RSITITRRVKN 726
            +YL FLCA GYD+  IS  + +  F CS   S+ +LNYPSIT+P L  + +TI R V N
Sbjct: 663 TDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTN 722

Query: 727 VGSPGTYKARIRKPSGISVWVKPKKLKFTKVGEEQSFKVLLKVKKQNVARNYVFGDLIWS 769
           VG P TY    R P+G S+ V P  L FTK+GE ++FKV+++       R Y FGDL W+
Sbjct: 723 VGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWT 782

BLAST of Spg006338 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.8e-174
Identity = 351/771 (45.53%), Postives = 486/771 (63.04%), Query Frame = 0

Query: 6   NSSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLESGD 65
           +S+AF LLL L     + +S+ + +Y+V+M       + P+    D H       L S  
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSSLRSIS 66

Query: 66  DFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGLEN 125
           D AE  + Y+Y   I+GF+  L  E A  L   P V+SV      +LHTTR+  F+GL+ 
Sbjct: 67  DSAE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 126

Query: 126 NNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQ-NQHDP 185
           +     +  ++ +A      ++G LDTGVWPESKS+S D+  GPIP  WKG C+   +  
Sbjct: 127 H-----TADLFPEAGSYSDVVVGVLDTGVWPESKSYS-DEGFGPIPSSWKGGCEAGTNFT 186

Query: 186 SFHCNRKLIGARYFHKGYESVVGRLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGASVFG 245
           +  CNRKLIGAR+F +GYES +G ++ S    SPRD++GHG+HT STA G+ V GAS+ G
Sbjct: 187 ASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLG 246

Query: 246 LGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNP 305
              GTA+G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG  + 
Sbjct: 247 YASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 306

Query: 306 LFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLG 365
            + D VAIG+F A++ GI+V CSAGN+GP++ S++N+APW  TVGA T+DR FP+  +LG
Sbjct: 307 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 366

Query: 366 NKKWIEGESL-SPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVC 425
           N K   G SL   EALP+ K  P + A +     ++     LC  GTL P K KGKI++C
Sbjct: 367 NGKNFTGVSLFKGEALPD-KLLPFIYAGNA----SNATNGNLCMTGTLIPEKVKGKIVMC 426

Query: 426 LRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYINS 485
            RG NARV KG+    AG +GMILAN+ A+G E++AD H+LPA+ +    G  +  Y+ +
Sbjct: 427 DRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT 486

Query: 486 TKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEGP 545
              P A I+   T +G++P+P +AAFSS GPN++TP ILKPD+ APGV+++AA+T A GP
Sbjct: 487 DPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGP 546

Query: 546 TNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFE 605
           T    D RRV FN ISGTSMSCPHVSG+A LL++++P WSPAAI+SA+MTTA     + +
Sbjct: 547 TGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 606

Query: 606 PLLNASYSK-ATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDG 665
           PLL+ +  K +TPF++GAGH+ P  AT+PGL+YD+  ++YL FLCAL Y   QI   S  
Sbjct: 607 PLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR 666

Query: 666 PFNC--SKPISLTNLNYPSITVPKLS-RSITITRRVKNVGSPGTYKARI-RKPSGISVWV 725
            + C  SK  S+ +LNYPS  V      +   TR V +VG  GTY  ++  + +G+ + V
Sbjct: 667 NYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISV 726

Query: 726 KPKKLKFTKVGEEQSFKVLLKVKKQNVARNYVFGDLIWSDGKHHVRSPIVV 768
           +P  L F +  E++S+ V   V     + +  FG + WSDGKH V SP+ +
Sbjct: 727 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Spg006338 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 7.7e-170
Identity = 354/798 (44.36%), Postives = 471/798 (59.02%), Query Frame = 0

Query: 7   SSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLESGDD 66
           +S F LL L+   L    +  K+ Y+VY G H   G K    + + HH  L+   ES +D
Sbjct: 5   TSLFPLLFLI--PLLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQSVKESEED 64

Query: 67  FAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRK--LHTTRSWKFMGLE 126
            A   + YSY   INGFAA L  + A++L K  +VVSVF +  RK   HTTRSW+F+GLE
Sbjct: 65  -ARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLE 124

Query: 127 N--------------NNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPI 186
                          ++      +  KKA+ G+G I+G LD+GVWPESKSF +D  +GP+
Sbjct: 125 EEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSF-NDKGMGPV 184

Query: 187 PFRWKGICQ-NQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFN----SPRDNEGHGSH 246
           P  WKGICQ      S HCNRK+IGARY+ KGYE   G  N++ N    SPRD +GHGSH
Sbjct: 185 PKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSH 244

Query: 247 TLSTAGGNFVAGASVF-GLGQGTAKGGSPRARVAAYKVCW-----PPAAGNECFDADILA 306
           T STA G  V GAS   G  +G+A GG+P AR+A YK CW         GN C + D+LA
Sbjct: 245 TASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLA 304

Query: 307 AFDMAIHDGVDVLSISLG-GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTN 366
           A D AI DGV V+SIS+G  +P P   D +A+G+ HAVK  IVV  SAGNSGP  G+++N
Sbjct: 305 AIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSN 364

Query: 367 IAPWQITVGASTMDRKFPSGVVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASV 426
           +APW ITVGAST+DR F  G+VLGN   I+ +S++  A    KF PL+ A++V +   ++
Sbjct: 365 LAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIAL 424

Query: 427 NEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGALGMILANSEASGNEILAD 486
           NE   C   +L P    GK+++CLRG  +R+ KG +   AG  GMIL N  A+GNE+ +D
Sbjct: 425 NETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSD 484

Query: 487 PHVLPASHINYTDGVTVFEYINSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPE 546
            H +P + +  T    + EYI + K P A+I    T    + AP M  FSS GPN V P 
Sbjct: 485 SHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPN 544

Query: 547 ILKPDVTAPGVSVIAAYTEAEGPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYP 606
           ILKPD+TAPG+ ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LL+ ++P
Sbjct: 545 ILKPDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHP 604

Query: 607 HWSPAAIKSAIMTTASTLDNNFEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVK 666
            WS AAI+SA+MTTA   ++  +P+ + +   A PF  G+GH  P  A DPGLVYD   +
Sbjct: 605 KWSSAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYR 664

Query: 667 EYLLFLCALGYDEAQISQFSDGPFNC-SKPISLTNLNYPSITVPKLSRSITITRRVKNVG 726
            YLL+ C++           D  F C SK     N NYPSI VP L +++T+ R V NVG
Sbjct: 665 AYLLYGCSVNITNI------DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVG 724

Query: 727 ---SPGTYKARIRKPSGISVWVKPKKLKFTKVGEEQSFKVLLKVKKQNVAR-----NYVF 768
              S  TY   ++ PSGISV   P  L F ++G++Q FK+++K  K  V        Y F
Sbjct: 725 TGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQF 784

BLAST of Spg006338 vs. ExPASy TrEMBL
Match: A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)

HSP 1 Score: 1377.1 bits (3563), Expect = 0.0e+00
Identity = 685/774 (88.50%), Postives = 726/774 (93.80%), Query Frame = 0

Query: 1   MKQRTNSSAFLLL---LLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELL 60
           MK  T SS+  LL   LLLSSSLFT TSAIKRSYVVYMGAHSHGGRKPAD+VADSHH+ L
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  RPFLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRS 120
           +PFL+SG++F +DVIFYSYTRHINGFAAMLEDEVAAQLAKHP+VVSVFLNRGRKLHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WKFMGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGI 180
           W+FMGLEN NGVI SESIWKKARFGE TIIGNLDTGVW ESKSFS DD++GPIP RWKGI
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFS-DDEMGPIPHRWKGI 180

Query: 181 CQNQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAG 240
           CQNQ+DPSFHCNRKLIGARYF+KGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAG
Sbjct: 181 CQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAG 240

Query: 241 ASVFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLG 300
           ASVFGLG+GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFD+AIHDGVDVLS+SLG
Sbjct: 241 ASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLG 300

Query: 301 GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS 360
           GDPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKFPS
Sbjct: 301 GDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPS 360

Query: 361 GVVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGK 420
            VVLGNKK IEGESLS EALP+KK YPLM+AADVRLANAS +EAQLCKAGTLDPMKAKGK
Sbjct: 361 LVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGK 420

Query: 421 ILVCLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFE 480
           ILVCLRGDNARVDKGEQALLAGA GMILAN+E SGNEILADPHVLPA HINYTDG  VF 
Sbjct: 421 ILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFA 480

Query: 481 YINSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTE 540
           YINSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNTVTPEILKPD+TAPG+SVIAAYTE
Sbjct: 481 YINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTE 540

Query: 541 AEGPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD 600
           AEGPTNQEFD+RRVPFNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD
Sbjct: 541 AEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD 600

Query: 601 NNFEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQF 660
           NNFEPLLNASY  ATPFNYGAGH+HPNGATDPGLVYDI+V EYL FLCALGYD+AQISQF
Sbjct: 601 NNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQF 660

Query: 661 SDGPFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVK 720
           SDGPFNCS+PISLTNLNYPSITVPKLSRSITITRR+KNVGSPGTY+A+IRKP+GISVWVK
Sbjct: 661 SDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVK 720

Query: 721 PKKLKFTKVGEEQSFKVLLKVKKQNVA-RNYVFGDLIWSDGKHHVRSPIVVKAV 771
           PKKL FTK+GEE SFKV +KVK   VA +NYV+GDLIWSDGKHHVRSPIVVK V
Sbjct: 721 PKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of Spg006338 vs. ExPASy TrEMBL
Match: A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 683/768 (88.93%), Postives = 724/768 (94.27%), Query Frame = 0

Query: 6   NSSAFL--LLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLES 65
           +SS FL  L LLLSSSLFT TSAIKRSYVVYMGAHSHGGRKPAD+VADSHH+ L+PFL+S
Sbjct: 7   SSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFLQPFLKS 66

Query: 66  GDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGL 125
           G++F +DVIFYSYTRHINGFAAMLEDEVAAQLAKHP+VVSVFLNRGRKLHTTRSW+FMGL
Sbjct: 67  GEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGL 126

Query: 126 ENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQNQHD 185
           EN NGVI SESIWKKARFGE TIIGNLDTGVW ESKSFS DD++GPIP RWKGICQNQ+D
Sbjct: 127 ENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFS-DDEMGPIPHRWKGICQNQND 186

Query: 186 PSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGL 245
           PSFHCNRKLIGARYF+KGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGASVFGL
Sbjct: 187 PSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGL 246

Query: 246 GQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNPL 305
           G+GTAKGGSPRARVAAYKVCWPP AGNECFDADILAAFD+AIHDGVDVLS+SLGGDPNPL
Sbjct: 247 GKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGGDPNPL 306

Query: 306 FNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLGN 365
           FNDSVAIGSFHA+KHGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKFPS VVLGN
Sbjct: 307 FNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGN 366

Query: 366 KKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVCLR 425
           KK IEGESLS EALP+KK YPLM+AADVRLANAS +EAQLCKAGTLDPMKAKGKILVCLR
Sbjct: 367 KKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLR 426

Query: 426 GDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYINSTK 485
           GDNARVDKGEQALLAGA GMILAN+E SGNEILADPHVLPA HINYTDG  VF YINSTK
Sbjct: 427 GDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAYINSTK 486

Query: 486 LPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEGPTN 545
            P AYIT ATTQLGIRPAPFMAAFSSVGPNTVTPEILKPD+TAPG+SVIAAYTEAEGPTN
Sbjct: 487 FPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTN 546

Query: 546 QEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPL 605
           QEFD+RRVPFNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPL
Sbjct: 547 QEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPL 606

Query: 606 LNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDGPFN 665
           LNASY  ATPFNYGAGH+HPNGATDPGLVYDI+V EYL FLCALGYD+AQISQFSDGPFN
Sbjct: 607 LNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFSDGPFN 666

Query: 666 CSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKKLKF 725
           CS+PISLTNLNYPSITVPKLSRSITITRR+KNVGSPGTY+A+IRKP+GISVWVKPKKL F
Sbjct: 667 CSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKPKKLNF 726

Query: 726 TKVGEEQSFKVLLKVKKQNVA-RNYVFGDLIWSDGKHHVRSPIVVKAV 771
           TK+GEE SFKV +KVK   VA +NYV+GDLIWSDGKHHVRSPIVVK V
Sbjct: 727 TKLGEELSFKVFMKVKGHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of Spg006338 vs. ExPASy TrEMBL
Match: A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)

HSP 1 Score: 1367.8 bits (3539), Expect = 0.0e+00
Identity = 679/772 (87.95%), Postives = 726/772 (94.04%), Query Frame = 0

Query: 1   MKQRTNSSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPF 60
           MKQ T  S   LLLLLSS L T T+AIKRSYVVYMGAHSHGGRKPADIVADSH ELLRP+
Sbjct: 23  MKQPTYPSTTFLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADSHRELLRPY 82

Query: 61  LESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKF 120
           LESG++FAEDVIFYSYTRHINGFAAMLEDEVAA+LAKHP+VVSVF NRGR+LHTTRSW+F
Sbjct: 83  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRLHTTRSWEF 142

Query: 121 MGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQN 180
           M LENN+GVI+SESIWKKARFGE TIIGNLDTGVWPESKSFSD DDLGPIP RW+GICQN
Sbjct: 143 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 202

Query: 181 QHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           Q+DPSFHCNRKLIGARYF+KGY+SVVG LNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 203 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 262

Query: 241 FGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDP 300
           FGLG+G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVLS+SLGGDP
Sbjct: 263 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 322

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVV 360
           NPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS VV
Sbjct: 323 NPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 382

Query: 361 LGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILV 420
           LGNKK IEGESL+PE LP++KFYPLMSAADVRLANAS +EAQLCKAGTLDP KAKGKILV
Sbjct: 383 LGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 442

Query: 421 CLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYIN 480
           CLRGDNARVDKGEQA+LAGA+GMILAN+E SGNEILADPHVLPASHIN+TDGV VF YIN
Sbjct: 443 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 502

Query: 481 STKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEG 540
           STK P AYIT ATTQLG RPAPFMAAFSSVGP+T+TPEILKPDVTAPG+SVIAAYTEAEG
Sbjct: 503 STKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTEAEG 562

Query: 541 PTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 600
           PTNQE+D RRV FNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF
Sbjct: 563 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 622

Query: 601 EPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDG 660
           +PLLNASYS ATPFNYGAGHIHPN ATDPGLVYDI + EYL FLCALGY++AQ+SQFS+G
Sbjct: 623 KPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKAQLSQFSNG 682

Query: 661 PFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKK 720
           PFNCS+PISLTNLNYPSITVP LSRSITITRR+KNVGSPGTYKA IR+P+GISV VKPKK
Sbjct: 683 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGISVSVKPKK 742

Query: 721 LKFTKVGEEQSFKVLLKVKKQNVAR--NYVFGDLIWSDGKHHVRSPIVVKAV 771
           L FT++GEE SFKVL+KVK+ NV R  NYV+GDLIWSDGKHHVRSPIVV A+
Sbjct: 743 LSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of Spg006338 vs. ExPASy TrEMBL
Match: A0A6J1GL32 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111455314 PE=3 SV=1)

HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 677/772 (87.69%), Postives = 730/772 (94.56%), Query Frame = 0

Query: 1   MKQRTNSSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPF 60
           MKQ T+ SA +LLLLLSS L T T+AIKRSYVVYMGAHSHGGRKPA+IVADSH +LLRP+
Sbjct: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVADSHRDLLRPY 60

Query: 61  LESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKF 120
           LESG++FAEDVIFYSYTRHINGFAAMLED+VAA+LA+HP+VVSVF NRGR+LHTTRSW+F
Sbjct: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDQVAARLAQHPEVVSVFPNRGRRLHTTRSWEF 120

Query: 121 MGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQN 180
           M LENN+GVI+SESIWKKARFGE TIIGNLDTGVWPESKSFSD DDLGPIP RW+GICQN
Sbjct: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 180

Query: 181 QHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           Q+DPSFHCNRKLIGARYF+KGY+SVVG LNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 181 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240

Query: 241 FGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDP 300
           FGLG+G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD+AIHDGVDVLS+SLGGDP
Sbjct: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVV 360
           NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS VV
Sbjct: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360

Query: 361 LGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILV 420
           LGNKK IEGESL+PE LP++KFYPLMSAA+VRLANAS +EAQLCKAGTLDP KAKGKILV
Sbjct: 361 LGNKKQIEGESLTPEGLPSRKFYPLMSAANVRLANASAHEAQLCKAGTLDPKKAKGKILV 420

Query: 421 CLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYIN 480
           CLRGDNARVDKGEQA+LAGA+GMILAN+E SGNEILADPHVLPASHIN+TDGV VF Y+N
Sbjct: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYVN 480

Query: 481 STKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEG 540
           STK P AYIT ATTQLGIRPAPFMAAFSSVGP+T+TPEILKPDVTAPG+SVIAAYT AEG
Sbjct: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540

Query: 541 PTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 600
           PTNQE+D RRV FNS+SGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAI+TTASTLDNNF
Sbjct: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIVTTASTLDNNF 600

Query: 601 EPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDG 660
           +PLLNASYS ATPFNYGAGHIHPN ATDPGLVYDI V EYL FLCALGY++AQ+SQFS+G
Sbjct: 601 KPLLNASYSVATPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660

Query: 661 PFNCSKPISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKK 720
           PFNCS+PISLTNLNYPSITVP LSRSITITRR+KNVGSPGTYKA+IRKP+GISV VKPKK
Sbjct: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAKIRKPAGISVSVKPKK 720

Query: 721 LKFTKVGEEQSFKVLLKVKKQNVAR--NYVFGDLIWSDGKHHVRSPIVVKAV 771
           L FT++GEE SFKVL+KVK+ NV R  NYV+GDLIWSDGKHHVRSPIVV AV
Sbjct: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of Spg006338 vs. ExPASy TrEMBL
Match: A0A0A0K313 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1)

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 650/737 (88.20%), Postives = 695/737 (94.30%), Query Frame = 0

Query: 35  MGAHSHGGRKPADIVADSHHELLRPFLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQ 94
           MGAHSHGG+KPAD+VA+SHHE L+PFL+SG++F +DVIFYSYTRHINGFAAMLEDEVA Q
Sbjct: 1   MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 60

Query: 95  LAKHPKVVSVFLNRGRKLHTTRSWKFMGLENNNGVITSESIWKKARFGEGTIIGNLDTGV 154
           LAKHPKVVSVFLNRGRKLHTTRSW+FMGLEN NGVI SESIWKKARFGE TIIGNLDTGV
Sbjct: 61  LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV 120

Query: 155 WPESKSFSDDDDLGPIPFRWKGICQNQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFN 214
           W ESKSFS DD+ GPIP RWKGICQNQ DPSFHCNRKLIGARYF+KGY SVVG LNSSF+
Sbjct: 121 WAESKSFS-DDEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFH 180

Query: 215 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFD 274
           SPRD EGHGSHTLSTAGGNFVAGASVFGLG+GTAKGGSPRARVAAYKVCWPP AGNECFD
Sbjct: 181 SPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFD 240

Query: 275 ADILAAFDMAIHDGVDVLSISLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG 334
           ADILAAFD AIHDGVDVLS+SLGGDPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG
Sbjct: 241 ADILAAFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAG 300

Query: 335 SVTNIAPWQITVGASTMDRKFPSGVVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLA 394
           +VTN+APWQITVGASTMDRKFPS VVLGN+K IEGESLS +ALP+KK YPLM+AADVRLA
Sbjct: 301 TVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLA 360

Query: 395 NASVNEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGALGMILANSEASGNE 454
           NASV+EAQLCKAGTL+PMKAKGKILVCLRGDNARVDKGEQALLAGA GMILAN+E SGNE
Sbjct: 361 NASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNE 420

Query: 455 ILADPHVLPASHINYTDGVTVFEYINSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNT 514
           ILADPHVLPASHIN+TDG  VF YINSTK P AYIT ATTQLGIRPAPFMAAFSSVGPNT
Sbjct: 421 ILADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNT 480

Query: 515 VTPEILKPDVTAPGVSVIAAYTEAEGPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLR 574
           VTPEILKPD+TAPG+SVIAAYTEAEGPTNQEFD+RR+PFNS+SGTSMSCPHVSGIAGLL+
Sbjct: 481 VTPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLK 540

Query: 575 TLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYD 634
           TLYPHWSPAAIKSAIMTTAS LDNNFEPLLNASYS A+PFNYGAGH+HPNGA DPGLVYD
Sbjct: 541 TLYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYD 600

Query: 635 IEVKEYLLFLCALGYDEAQISQFSDGPFNCSKPISLTNLNYPSITVPKLSRSITITRRVK 694
           IEV EYL FLCALGY++AQISQFS+GPFNCS PIS TNLNYPSITVPKLSRSITITRR+K
Sbjct: 601 IEVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLK 660

Query: 695 NVGSPGTYKARIRKPSGISVWVKPKKLKFTKVGEEQSFKVLLKVKKQNVA-RNYVFGDLI 754
           NVGSPGTYKA IRKP+GISVWVKPKKL FT++GEE SFKVL+KVK++ VA +NYV+GDLI
Sbjct: 661 NVGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLI 720

Query: 755 WSDGKHHVRSPIVVKAV 771
           WSDGKHHVRSPIVVK V
Sbjct: 721 WSDGKHHVRSPIVVKVV 736

BLAST of Spg006338 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 985.3 bits (2546), Expect = 2.7e-287
Identity = 493/771 (63.94%), Postives = 600/771 (77.82%), Query Frame = 0

Query: 5   TNSSAFLLLLLL--SSSLFTLTSAIKRSYVVYMGAHSHGG---RKPADIVADSHHELLRP 64
           T++ +FLLLLLL   SS   L S    SYVVY GAHSH G       D V ++H++ L  
Sbjct: 4   THNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGS 63

Query: 65  FLESGDDFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWK 124
           F  S +  A D IFYSYT+HINGFAA L+ ++A +++KHP+VVSVF N+  KLHTTRSW 
Sbjct: 64  FTGSRER-ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWD 123

Query: 125 FMGLENNNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQ 184
           F+GLE+N+  + S SIW+KARFGE TII NLDTGVWPESKSF  D+ LGPIP RWKGICQ
Sbjct: 124 FLGLEHNS-YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFR-DEGLGPIPSRWKGICQ 183

Query: 185 NQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFNSPRDNEGHGSHTLSTAGGNFVAGAS 244
           NQ D +FHCNRKLIGARYF+KGY + VG LNSSF+SPRD +GHGSHTLSTA G+FV G S
Sbjct: 184 NQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVS 243

Query: 245 VFGLGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGD 304
           +FG G GTAKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+S+SLGG+
Sbjct: 244 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 303

Query: 305 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGV 364
           P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +V+N+APWQITVGASTMDR+F S +
Sbjct: 304 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 363

Query: 365 VLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKIL 424
           VLGN K  +G+SLS  ALP+ KFYP+M++ + +  NAS  +AQLCK G+LDP+K KGKIL
Sbjct: 364 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 423

Query: 425 VCLRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYI 484
           VCLRG N RV+KG    L G +GM+L N+  +GN++LADPHVLPA+ +   D   V  YI
Sbjct: 424 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 483

Query: 485 NSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAE 544
           + TK P+A+IT + T LG++PAP MA+FSS GP+ V P+ILKPD+TAPGVSVIAAYT A 
Sbjct: 484 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 543

Query: 545 GPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNN 604
            PTN++FD RR+ FN+ISGTSMSCPH+SGIAGLL+T YP WSPAAI+SAIMTTA+ +D+ 
Sbjct: 544 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 603

Query: 605 FEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSD 664
             P+ NA+  KATPF++GAGH+ PN A +PGLVYD+ +K+YL FLC+LGY+ +QIS FS 
Sbjct: 604 PGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG 663

Query: 665 GPFNCSKP-ISLTNLNYPSITVPKLSRS-ITITRRVKNVGSPGTYKARIRKPSGISVWVK 724
             F CS P ISL NLNYPSITVP L+ S +T++R VKNVG P  Y  ++  P G+ V VK
Sbjct: 664 NNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVK 723

Query: 725 PKKLKFTKVGEEQSFKVLLKVKKQNVARNYVFGDLIWSDGKHHVRSPIVVK 769
           P  L FTKVGE+++FKV+L   K NVA+ YVFG+L+WSD KH VRSPIVVK
Sbjct: 724 PTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

BLAST of Spg006338 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 892.9 bits (2306), Expect = 1.8e-259
Identity = 451/761 (59.26%), Postives = 572/761 (75.16%), Query Frame = 0

Query: 13  LLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPA---DIVADSHHELLRPFLESGDDFAE 72
           LLLL +  F+   A+K+SY+VY+G+H+H  +  +   D VA SH   L  F+ S ++ A+
Sbjct: 24  LLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN-AK 83

Query: 73  DVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGLENNNGV 132
           + IFYSY RHINGFAA+L++  AA++AKHP VVSVF N+GRKLHTT SW FM L   NGV
Sbjct: 84  EAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM-LLAKNGV 143

Query: 133 ITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQNQHDPSFHCN 192
           +   S+W KA +GE TII NLDTGVWPESKSFS D+  G +P RWKG C         CN
Sbjct: 144 VHKSSLWNKAGYGEDTIIANLDTGVWPESKSFS-DEGYGAVPARWKGRCHK----DVPCN 203

Query: 193 RKLIGARYFHKGYESVVG-RLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGQGTA 252
           RKLIGARYF+KGY +  G   N+S+ + RD++GHGSHTLSTA GNFV GA+VFG+G GTA
Sbjct: 204 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 263

Query: 253 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNPLFNDSV 312
            GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DGVDVLS S+GGD     +D +
Sbjct: 264 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 323

Query: 313 AIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLGNKKWIE 372
           AIGSFHAVK+G+ V+CSAGNSGP +G+V+N+APW ITVGAS+MDR+F + V L N +  +
Sbjct: 324 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 383

Query: 373 GESLSPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVCLRGDNAR 432
           G SLS + LP +K Y L+SAAD  +AN +V +A LCK G+LDP K KGKILVCLRGDNAR
Sbjct: 384 GTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 443

Query: 433 VDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYINSTKLPVAY 492
           VDKG QA  AGA GM+L N +ASGNEI++D HVLPAS I+Y DG T+F Y++STK P  Y
Sbjct: 444 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 503

Query: 493 ITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEGPTNQEFDH 552
           I   T  L  +PAPFMA+FSS GPNT+TP ILKPD+TAPGV++IAA+TEA GPT+ + D+
Sbjct: 504 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 563

Query: 553 RRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY 612
           RR PFN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAI+SAIMTT+ T +N  +P+++ S+
Sbjct: 564 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 623

Query: 613 SKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDGP-FNCSKP 672
            KA PF+YG+GH+ PN A  PGLVYD+   +YL FLCA+GY+   +  F++ P + C + 
Sbjct: 624 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 683

Query: 673 ISLTNLNYPSITVPKLSRSITITRRVKNVGSPGTYKARIRKPSGISVWVKPKKLKFTKVG 732
            +L + NYPSITVP L+ SIT+TR++KNVG P TY AR R+P G+ V V+PK+L F K G
Sbjct: 684 ANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 743

Query: 733 EEQSFKVLLKVKKQNVARNYVFGDLIWSDGKHHVRSPIVVK 769
           E + F++ L+      +  YVFG+L W+D  H+VRSPIVV+
Sbjct: 744 EVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQ 775

BLAST of Spg006338 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 614.4 bits (1583), Expect = 1.3e-175
Identity = 351/771 (45.53%), Postives = 486/771 (63.04%), Query Frame = 0

Query: 6   NSSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLESGD 65
           +S+AF LLL L     + +S+ + +Y+V+M       + P+    D H       L S  
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSSLRSIS 66

Query: 66  DFAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGLEN 125
           D AE  + Y+Y   I+GF+  L  E A  L   P V+SV      +LHTTR+  F+GL+ 
Sbjct: 67  DSAE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 126

Query: 126 NNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGICQ-NQHDP 185
           +     +  ++ +A      ++G LDTGVWPESKS+S D+  GPIP  WKG C+   +  
Sbjct: 127 H-----TADLFPEAGSYSDVVVGVLDTGVWPESKSYS-DEGFGPIPSSWKGGCEAGTNFT 186

Query: 186 SFHCNRKLIGARYFHKGYESVVGRLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGASVFG 245
           +  CNRKLIGAR+F +GYES +G ++ S    SPRD++GHG+HT STA G+ V GAS+ G
Sbjct: 187 ASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLG 246

Query: 246 LGQGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNP 305
              GTA+G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG  + 
Sbjct: 247 YASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 306

Query: 306 LFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLG 365
            + D VAIG+F A++ GI+V CSAGN+GP++ S++N+APW  TVGA T+DR FP+  +LG
Sbjct: 307 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 366

Query: 366 NKKWIEGESL-SPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVC 425
           N K   G SL   EALP+ K  P + A +     ++     LC  GTL P K KGKI++C
Sbjct: 367 NGKNFTGVSLFKGEALPD-KLLPFIYAGNA----SNATNGNLCMTGTLIPEKVKGKIVMC 426

Query: 426 LRGDNARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYINS 485
            RG NARV KG+    AG +GMILAN+ A+G E++AD H+LPA+ +    G  +  Y+ +
Sbjct: 427 DRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT 486

Query: 486 TKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAEGP 545
              P A I+   T +G++P+P +AAFSS GPN++TP ILKPD+ APGV+++AA+T A GP
Sbjct: 487 DPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGP 546

Query: 546 TNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFE 605
           T    D RRV FN ISGTSMSCPHVSG+A LL++++P WSPAAI+SA+MTTA     + +
Sbjct: 547 TGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 606

Query: 606 PLLNASYSK-ATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFSDG 665
           PLL+ +  K +TPF++GAGH+ P  AT+PGL+YD+  ++YL FLCAL Y   QI   S  
Sbjct: 607 PLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR 666

Query: 666 PFNC--SKPISLTNLNYPSITVPKLS-RSITITRRVKNVGSPGTYKARI-RKPSGISVWV 725
            + C  SK  S+ +LNYPS  V      +   TR V +VG  GTY  ++  + +G+ + V
Sbjct: 667 NYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISV 726

Query: 726 KPKKLKFTKVGEEQSFKVLLKVKKQNVARNYVFGDLIWSDGKHHVRSPIVV 768
           +P  L F +  E++S+ V   V     + +  FG + WSDGKH V SP+ +
Sbjct: 727 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Spg006338 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 599.0 bits (1543), Expect = 5.5e-171
Identity = 354/798 (44.36%), Postives = 471/798 (59.02%), Query Frame = 0

Query: 7   SSAFLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLESGDD 66
           +S F LL L+   L    +  K+ Y+VY G H   G K    + + HH  L+   ES +D
Sbjct: 5   TSLFPLLFLI--PLLASCAEEKQVYIVYFGEHK--GDKAFHEIEEHHHSYLQSVKESEED 64

Query: 67  FAEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRK--LHTTRSWKFMGLE 126
            A   + YSY   INGFAA L  + A++L K  +VVSVF +  RK   HTTRSW+F+GLE
Sbjct: 65  -ARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLE 124

Query: 127 N--------------NNGVITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPI 186
                          ++      +  KKA+ G+G I+G LD+GVWPESKSF +D  +GP+
Sbjct: 125 EEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSF-NDKGMGPV 184

Query: 187 PFRWKGICQ-NQHDPSFHCNRKLIGARYFHKGYESVVGRLNSSFN----SPRDNEGHGSH 246
           P  WKGICQ      S HCNRK+IGARY+ KGYE   G  N++ N    SPRD +GHGSH
Sbjct: 185 PKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSH 244

Query: 247 TLSTAGGNFVAGASVF-GLGQGTAKGGSPRARVAAYKVCW-----PPAAGNECFDADILA 306
           T STA G  V GAS   G  +G+A GG+P AR+A YK CW         GN C + D+LA
Sbjct: 245 TASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLA 304

Query: 307 AFDMAIHDGVDVLSISLG-GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTN 366
           A D AI DGV V+SIS+G  +P P   D +A+G+ HAVK  IVV  SAGNSGP  G+++N
Sbjct: 305 AIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSN 364

Query: 367 IAPWQITVGASTMDRKFPSGVVLGNKKWIEGESLSPEALPNKKFYPLMSAADVRLANASV 426
           +APW ITVGAST+DR F  G+VLGN   I+ +S++  A    KF PL+ A++V +   ++
Sbjct: 365 LAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIAL 424

Query: 427 NEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGALGMILANSEASGNEILAD 486
           NE   C   +L P    GK+++CLRG  +R+ KG +   AG  GMIL N  A+GNE+ +D
Sbjct: 425 NETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSD 484

Query: 487 PHVLPASHINYTDGVTVFEYINSTKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPE 546
            H +P + +  T    + EYI + K P A+I    T    + AP M  FSS GPN V P 
Sbjct: 485 SHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPN 544

Query: 547 ILKPDVTAPGVSVIAAYTEAEGPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYP 606
           ILKPD+TAPG+ ++AA++ A+ P+    D R   +N  SGTSMSCPHV+G   LL+ ++P
Sbjct: 545 ILKPDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHP 604

Query: 607 HWSPAAIKSAIMTTASTLDNNFEPLLNASYSKATPFNYGAGHIHPNGATDPGLVYDIEVK 666
            WS AAI+SA+MTTA   ++  +P+ + +   A PF  G+GH  P  A DPGLVYD   +
Sbjct: 605 KWSSAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYR 664

Query: 667 EYLLFLCALGYDEAQISQFSDGPFNC-SKPISLTNLNYPSITVPKLSRSITITRRVKNVG 726
            YLL+ C++           D  F C SK     N NYPSI VP L +++T+ R V NVG
Sbjct: 665 AYLLYGCSVNITNI------DPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVG 724

Query: 727 ---SPGTYKARIRKPSGISVWVKPKKLKFTKVGEEQSFKVLLKVKKQNVAR-----NYVF 768
              S  TY   ++ PSGISV   P  L F ++G++Q FK+++K  K  V        Y F
Sbjct: 725 TGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQF 784

BLAST of Spg006338 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 592.0 bits (1525), Expect = 6.7e-169
Identity = 346/781 (44.30%), Postives = 478/781 (61.20%), Query Frame = 0

Query: 10  FLLLLLLSSSLFTLTSAIKRSYVVYMGAHSHGGRKPADIVADSHHELLRPFLESGDDFAE 69
           F  LL LSS   + +S+   +Y+V++   +    KP+  +  +H       L S      
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEA----KPS--IFPTHFHWYTSSLASLTSSPP 66

Query: 70  DVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSVFLNRGRKLHTTRSWKFMGLENNNGV 129
            +I ++Y    +GF+A L  + A+QL  HP V+SV   + R LHTTRS +F+GL + +  
Sbjct: 67  SII-HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD-- 126

Query: 130 ITSESIWKKARFGEGTIIGNLDTGVWPESKSFSDDDDLGPIPFRWKGIC-QNQHDPSFHC 189
                + +++ FG   +IG +DTGVWPE  SF DD  LGP+P +WKG C  +Q  P   C
Sbjct: 127 --KAGLLEESDFGSDLVIGVIDTGVWPERPSF-DDRGLGPVPIKWKGQCIASQDFPESAC 186

Query: 190 NRKLIGARYFHKGYESVVGRLN--SSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGQG 249
           NRKL+GAR+F  GYE+  G++N  + F SPRD++GHG+HT S + G +V  AS  G   G
Sbjct: 187 NRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 246

Query: 250 TAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDMAIHDGVDVLSISLGGDPNPLFND 309
            A G +P+AR+AAYKVCW     + C+D+DILAAFD A+ DGVDV+S+S+GG   P + D
Sbjct: 247 VAAGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLD 306

Query: 310 SVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSGVVLGNKKW 369
           ++AIG+F A+  GI V  SAGN GP A +VTN+APW  TVGA T+DR FP+ V LGN K 
Sbjct: 307 AIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKM 366

Query: 370 IEGESL-SPEALPNKKFYPLMSAADVRLANASVNEAQLCKAGTLDPMKAKGKILVCLRGD 429
           I G S+     L   + YPL+      L       + LC  G+LDP   KGKI++C RG 
Sbjct: 367 ISGVSVYGGPGLDPGRMYPLVYGGS--LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGI 426

Query: 430 NARVDKGEQALLAGALGMILANSEASGNEILADPHVLPASHINYTDGVTVFEYIN----- 489
           N+R  KGE     G LGMI+AN    G  ++AD HVLPA+ +  + G  +  YI+     
Sbjct: 427 NSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKS 486

Query: 490 -STKLPVAYITRATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDVTAPGVSVIAAYTEAE 549
            S+K P A I    T+LGIRPAP +A+FS+ GPN  TPEILKPDV APG++++AA+ +  
Sbjct: 487 RSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRI 546

Query: 550 GPTNQEFDHRRVPFNSISGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNN 609
           GP+    D+RR  FN +SGTSM+CPHVSG+A LL+  +P WSPAAI+SA++TTA T+DN+
Sbjct: 547 GPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNS 606

Query: 610 FEPLLNASY-SKATPFNYGAGHIHPNGATDPGLVYDIEVKEYLLFLCALGYDEAQISQFS 669
            EP+++ S  + ++  +YG+GH+HP  A DPGLVYDI   +Y+ FLC   Y    I   +
Sbjct: 607 GEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTIT 666

Query: 670 DGPFNCS---KPISLTNLNYPSITV-----PKLSRSITITRRVKNVG-SPGTYKARIRKP 729
               +C    +   + NLNYPS +V      +   S    R V NVG S   Y+ +IR P
Sbjct: 667 RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPP 726

Query: 730 SGISVWVKPKKLKFTKVGEEQSFKVLLK---VKKQNVARNYVFGDLIWSDGKHHVRSPIV 768
            G +V V+P+KL F +VG++ SF V +K   VK    A N   G ++WSDGK +V SP+V
Sbjct: 727 RGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLV 769