Spg006327 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg006327
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	O-methyltransferase
Location	scaffold4: 6202781 .. 6203653 (-)
RNA-Seq Expression	Spg006327
Synteny	Spg006327

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATATGGATTTGGAGGGTGGAAAAATGGCAGTAGTTGCAGGAGATGAGCTGCTGGAAGCTCAAGCCCACATATGGAATCACATGTTCAACTTCATAAACTCCATGTCTCTGAAATGCGCCGTTGAACTTGGCATAGCAGACGCCATCCACCGCCATGGCCCTCACCCCATGCCCCTCTCTCTACTTGTTTCAGCCCTCCAACTCCATCCCAATAAGACTCCTTTCCTTTCCCGCTTGATGCGTATATTGACTCACTCAGGCTTCTTCGTAGAAAAAGAATTAGAAGGATATATGCTGACCAATTCATCTCGTCTTCTTCTCAAAGACAACCCTTTGAGTGTAACTCCTTTCTTGCTTTCCATGCTCGACTCAGCCCTCGTGGAGCCATGGCAGTTTCTCTCTGTGTGGTTCCAAAGTGATGGCCCAACGCCATTCGATACGGCTCATGGCATGCCGTTTTGGGAATTCATGGGCAACAAGCCGAATGATGGTGACGTTTTTAATGCAGGCATTGCGAGTGATGCGAGGCTGGTCATGAGTGTTTTGTTGGGCAAATATAAAGGTGTTTTTGATGGAGTTGGCTCGCTGGTTGATGTTGGTGGTGGCACTGGAACTGTGGCCAAAGCCATTGCTGAGGCTTTTCCAGAAATTGAATGCACCGTGTTTGATCTTCCCCAAGTTGTGGGTGACTTGAAAGGGAAAGGAAAATTTAAATATGTTGAAGGAGATATGTTTAAAGCTATTCCTCCTGCTGATTCAATTCTTTTGAAGGTAAACATAATATTTTATTGTTATCCATACATGTCCCGAGTTCACATACTACAACAAATTTGGTCCTTAGAGTTTGGTCTCTATAGCTTTAAAAGTTAG

mRNA sequence

ATGAATATGGATTTGGAGGGTGGAAAAATGGCAGTAGTTGCAGGAGATGAGCTGCTGGAAGCTCAAGCCCACATATGGAATCACATGTTCAACTTCATAAACTCCATGTCTCTGAAATGCGCCGTTGAACTTGGCATAGCAGACGCCATCCACCGCCATGGCCCTCACCCCATGCCCCTCTCTCTACTTGTTTCAGCCCTCCAACTCCATCCCAATAAGACTCCTTTCCTTTCCCGCTTGATGCGTATATTGACTCACTCAGGCTTCTTCGTAGAAAAAGAATTAGAAGGATATATGCTGACCAATTCATCTCGTCTTCTTCTCAAAGACAACCCTTTGAGTGTAACTCCTTTCTTGCTTTCCATGCTCGACTCAGCCCTCGTGGAGCCATGGCAGTTTCTCTCTGTGTGGTTCCAAAGTGATGGCCCAACGCCATTCGATACGGCTCATGGCATGCCGTTTTGGGAATTCATGGGCAACAAGCCGAATGATGGTGACGTTTTTAATGCAGGCATTGCGAGTGATGCGAGGCTGGTCATGAGTGTTTTGTTGGGCAAATATAAAGGTGTTTTTGATGGAGTTGGCTCGCTGGTTGATGTTGGTGGTGGCACTGGAACTGTGGCCAAAGCCATTGCTGAGGCTTTTCCAGAAATTGAATGCACCGTGTTTGATCTTCCCCAAGTTGTGGGTGACTTGAAAGGGAAAGGAAAATTTAAATATGTTGAAGGAGATATGTTTAAAGCTATTCCTCCTGCTGATTCAATTCTTTTGAAGGTAAACATAATATTTTATTGTTATCCATACATGTCCCGAGTTCACATACTACAACAAATTTGGTCCTTAGAGTTTGGTCTCTATAGCTTTAAAAGTTAG

Coding sequence (CDS)

ATGAATATGGATTTGGAGGGTGGAAAAATGGCAGTAGTTGCAGGAGATGAGCTGCTGGAAGCTCAAGCCCACATATGGAATCACATGTTCAACTTCATAAACTCCATGTCTCTGAAATGCGCCGTTGAACTTGGCATAGCAGACGCCATCCACCGCCATGGCCCTCACCCCATGCCCCTCTCTCTACTTGTTTCAGCCCTCCAACTCCATCCCAATAAGACTCCTTTCCTTTCCCGCTTGATGCGTATATTGACTCACTCAGGCTTCTTCGTAGAAAAAGAATTAGAAGGATATATGCTGACCAATTCATCTCGTCTTCTTCTCAAAGACAACCCTTTGAGTGTAACTCCTTTCTTGCTTTCCATGCTCGACTCAGCCCTCGTGGAGCCATGGCAGTTTCTCTCTGTGTGGTTCCAAAGTGATGGCCCAACGCCATTCGATACGGCTCATGGCATGCCGTTTTGGGAATTCATGGGCAACAAGCCGAATGATGGTGACGTTTTTAATGCAGGCATTGCGAGTGATGCGAGGCTGGTCATGAGTGTTTTGTTGGGCAAATATAAAGGTGTTTTTGATGGAGTTGGCTCGCTGGTTGATGTTGGTGGTGGCACTGGAACTGTGGCCAAAGCCATTGCTGAGGCTTTTCCAGAAATTGAATGCACCGTGTTTGATCTTCCCCAAGTTGTGGGTGACTTGAAAGGGAAAGGAAAATTTAAATATGTTGAAGGAGATATGTTTAAAGCTATTCCTCCTGCTGATTCAATTCTTTTGAAGGTAAACATAATATTTTATTGTTATCCATACATGTCCCGAGTTCACATACTACAACAAATTTGGTCCTTAGAGTTTGGTCTCTATAGCTTTAAAAGTTAG

Protein sequence

MNMDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSLLVSALQLHPNKTPFLSRLMRILTHSGFFVEKELEGYMLTNSSRLLLKDNPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKGKFKYVEGDMFKAIPPADSILLKVNIIFYCYPYMSRVHILQQIWSLEFGLYSFKS

Homology

[Close]

BLAST of Spg006327 vs. NCBI nr
Match: XP_022952337.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 432.2 bits (1110), Expect = 3.6e-117
Identity = 211/268 (78.73%), Postives = 233/268 (86.94%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+LEGGKMA +AG+ELLEAQAHIWNH+FNFINSM+LKCA++LGI DAIHRHGPHPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLSR 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV------------EKELEGYMLTNSSRLLLKD 122
           LVSALQLHPNKT F+ RLMR+LTHSGFFV            E+E EGY+LTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLKH 120

Query: 123 NPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNA 182
           NP +VTPFLLSML  ALVEPWQ LS WFQS+  TPF TAHGMPFWEF+GNK  DG+ FNA
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNA 180

Query: 183 GIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVG 242
           G+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTV KAIAEAFP+IECTVFDLPQVVG
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQVVG 240

Query: 243 DLKGKGKFKYVEGDMFKAIPPADSILLK 259
           +LKG+G  KYVEGDMFKAIPPAD++LLK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADALLLK 268

[Close]

BLAST of Spg006327 vs. NCBI nr
Match: KAG6572268.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 422.5 bits (1085), Expect = 2.8e-114
Identity = 206/265 (77.74%), Postives = 229/265 (86.42%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+ EG KM ++AGDE+LEAQAHIWNH+FNFINSM+LKCAVELGI D IHRH PHPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFVEKEL---------EGYMLTNSSRLLLKDNPL 122
           LVSALQLH NKT F+ RLMR+LTHSGFFV ++L         EGY+LTNSSRLLLK NP 
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEGYVLTNSSRLLLKHNPC 120

Query: 123 SVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIA 182
           +VTPFLLSML  ALVEPWQ LS+WFQS+  TPF TAHGMPFWEFMG+KP  GD+FNAG+A
Sbjct: 121 AVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFMGSKPKHGDIFNAGMA 180

Query: 183 SDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLK 242
           SDA LVMSVLL K+KGVFDGV SLVDVGGGTGTVAKAIAEAFP+IECTVFDLPQVVG+LK
Sbjct: 181 SDAWLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQVVGELK 240

Query: 243 GKGKFKYVEGDMFKAIPPADSILLK 259
           G+G  KYVEGDMFKAIPPAD ++LK
Sbjct: 241 GEGNLKYVEGDMFKAIPPADVLVLK 265

[Close]

BLAST of Spg006327 vs. NCBI nr
Match: XP_022969140.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 422.2 bits (1084), Expect = 3.7e-114
Identity = 207/268 (77.24%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+LEGGKMA +AG+ELLEAQAHIWNH+FNFINSM+LKCA++LGI DAIHRH PHPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLSR 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV------------EKELEGYMLTNSSRLLLKD 122
           LVSALQLHPNKT F+ RLMR+LTHSGFFV            E+E EGY+LTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLKH 120

Query: 123 NPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNA 182
           NP   TPFLLSML  ALVEPWQ LS+WFQS+  TPF TAHGMP WEF+GNK  DG+ FNA
Sbjct: 121 NPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFNA 180

Query: 183 GIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVG 242
           G+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTVAKAIAEAFP+IECTV DLPQVVG
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQVVG 240

Query: 243 DLKGKGKFKYVEGDMFKAIPPADSILLK 259
           +LKG+G  KYVEGDMFKAIP AD++LLK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPSADALLLK 268

[Close]

BLAST of Spg006327 vs. NCBI nr
Match: XP_022952338.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 418.7 bits (1075), Expect = 4.1e-113
Identity = 205/268 (76.49%), Postives = 228/268 (85.07%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+ EG KM ++AGDE+LEAQAHIWNH+FNFINSM+LKCAVELGI D IHRH PHPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV------------EKELEGYMLTNSSRLLLKD 122
           LVSALQLH NKT F+ RLMR+LTHSGFFV            E+E +GY+LTNSSRLLLK 
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKH 120

Query: 123 NPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNA 182
           NP +VTPFLLSML  ALVEPWQ LS+WFQS+  TPF TAHGMPFWEF+GNK  DG+ FN 
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNE 180

Query: 183 GIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVG 242
           G+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTVAKAIAEAFP+IECTVFDLPQVVG
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQVVG 240

Query: 243 DLKGKGKFKYVEGDMFKAIPPADSILLK 259
           +LKG+G  KYVEGDMFKAIPPAD ++LK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADVLVLK 268

[Close]

BLAST of Spg006327 vs. NCBI nr
Match: XP_023554524.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.5 bits (1072), Expect = 9.1e-113
Identity = 204/266 (76.69%), Postives = 227/266 (85.34%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+ EG KM ++AGDE+LEAQAHIWNH+FNFINSM+LKCAVELGI DAIHRH PHPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDAIHRHAPHPMPLSH 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV----------EKELEGYMLTNSSRLLLKDNP 122
           LVSALQLH NKT F+ RLMR+LTHSGFFV          ++E +GY+LTNSSRLLLK NP
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVLQKLPKNNGKQQEEDGYVLTNSSRLLLKHNP 120

Query: 123 LSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGI 182
            +VTPFLLSML  ALVEPWQ LS WFQS  PTPF TAHGMPFWEF+G+K  DG+ FNAG+
Sbjct: 121 CAVTPFLLSMLQPALVEPWQLLSTWFQSQDPTPFHTAHGMPFWEFLGSKVKDGEGFNAGM 180

Query: 183 ASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDL 242
           ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTVAKAIA AFP+IECTVFDLPQVVG+L
Sbjct: 181 ASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAHAFPQIECTVFDLPQVVGEL 240

Query: 243 KGKGKFKYVEGDMFKAIPPADSILLK 259
           KG+G  KYVEGDMF AIPPAD ++LK
Sbjct: 241 KGEGNLKYVEGDMFNAIPPADVLVLK 266

[Close]

BLAST of Spg006327 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.3e-79
Identity = 148/252 (58.73%), Postives = 185/252 (73.41%), Query Frame = 0


Query: 17  ELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSLLVSALQLHPNKTPF 76
           E+L AQAHIWN +FNFINSMSLKCA++LGI D I+ HG  PM +S L  AL ++  K+P 
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKSPC 84

Query: 77  LSRLMRILTHSGFFVEK----------ELEGYMLTNSSRLLLKDNPLSVTPFLLSMLDSA 136
           + RLMRIL HSGFF  +          E EGY++T++S+LLLKDNP+SVTPFLL+MLD  
Sbjct: 85  VYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPV 144

Query: 137 LVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDARLVMSVLLGK 196
           + +PW FLS WFQ+  PTPFDTA+GM FW++  ++P     FN  +ASDARLV SV++ K
Sbjct: 145 MTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIEK 204

Query: 197 YKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKGKFKYVEGDMF 256
            KGVF+GV SLVDVGGGTGTVA +IA AFP I+CTVFDLP VV DL+G     +V GDMF
Sbjct: 205 CKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVGGDMF 264

Query: 257 KAIPPADSILLK 259
             +P  + +LLK
Sbjct: 265 VDVPATEVVLLK 275

[Close]

BLAST of Spg006327 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 292.7 bits (748), Expect = 4.5e-78
Identity = 150/262 (57.25%), Postives = 184/262 (70.23%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           MDL  G    V   ELL AQAH+WNH+FNFI SMSLKCA++LGI D IH HG  PM L  
Sbjct: 1   MDLANG----VISAELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPE 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFVE------KELEGYMLTNSSRLLLKDNPLSVT 122
           LV+ L +HP ++  + RLMRIL HSGF         KE EGY+LT++SRLLL D+ LS+ 
Sbjct: 61  LVAKLPVHPKRSQCVYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIR 120

Query: 123 PFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDA 182
           P +L+MLD  L +PW +LS WFQ+D PTPF TA+   FW++ G++P   + FN  +ASDA
Sbjct: 121 PLVLAMLDPILTKPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDA 180

Query: 183 RLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKG 242
           RL+ SVLL + +GVF G+ SLVDVGGGTG VAKAIA AFP + CTV DL  VV  L+G  
Sbjct: 181 RLLTSVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSK 240

Query: 243 KFKYVEGDMFKAIPPADSILLK 259
              Y  GDMF+AIPPAD+ILLK
Sbjct: 241 NLNYFAGDMFEAIPPADAILLK 257

[Close]

BLAST of Spg006327 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 4.8e-72
Identity = 135/265 (50.94%), Postives = 184/265 (69.43%), Query Frame = 0


Query: 1   MNMDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPL 60
           M++++  G     +G+ELL AQAHI+NH+FNFI+SM+LKCAVEL I + +H H P  + L
Sbjct: 7   MDLEIRNGNGYGDSGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTL 66

Query: 61  SLLVSALQLHPNKTPFLSRLMRILTHSGFFV------EKELEGYMLTNSSRLLLKDNPLS 120
           S LV ALQ+   K+  L RL+RIL HSGFF       E + EGY+ T SS+LLLK++P+S
Sbjct: 67  SELVQALQIPQAKSACLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKNHPMS 126

Query: 121 VTPFLLSMLDSALVEPWQFLSVWF-QSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIA 180
           ++P LL +++  +V P  F S WF +SD  TPF+  HG   W++ G  P+  ++FN  + 
Sbjct: 127 MSPCLLGLVNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMG 186

Query: 181 SDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLK 240
            + RL MSV+L + KG  +G+ SLVDVGGGTG V +AIAEAFP ++CTV DLPQVVG+LK
Sbjct: 187 CETRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLK 246

Query: 241 GKGKFKYVEGDMFKAIPPADSILLK 259
           G    ++V GDMF+ IPPAD + LK
Sbjct: 247 GSNNLEFVSGDMFQFIPPADVVFLK 271

[Close]

BLAST of Spg006327 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 132/255 (51.76%), Postives = 166/255 (65.10%), Query Frame = 0


Query: 17  ELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSLLVSALQLHPNKTPF 76
           ELLEAQAH+WNH++++INSMSLKCA++LGI DAIH+HG +P+ LS L  AL ++  K+  
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNINKAKSHG 71

Query: 77  LSRLMRILTHSGFF-------------VEKELEGYMLTNSSRLLLKDNPLSVTPFLLSML 136
           L RLMRIL HSGFF              E+E + Y LT +SRLLL+  PLSV PF L+M 
Sbjct: 72  LFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFALAMS 131

Query: 137 DSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDARLVMSVL 196
           D    E W  LS WF++D    FDT +GM F E+         +FN  +A DA  V S+L
Sbjct: 132 DPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVNSIL 191

Query: 197 LGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKGKFKYVEG 256
             + + +FDG+ S+VDVGGGTG  AK IA AFP +ECTV DLP VVG LKG     +V G
Sbjct: 192 TTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSENLSFVSG 251

Query: 257 DMFKAIPPADSILLK 259
           DMF  IP AD+I +K
Sbjct: 252 DMFDFIPHADAIFMK 265

[Close]

BLAST of Spg006327 vs. ExPASy Swiss-Prot
Match: Q93WU3 (Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-65
Identity = 127/262 (48.47%), Postives = 173/262 (66.03%), Query Frame = 0


Query: 2   NMDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLS 61
           NMD+       ++ ++LL+AQAH+WNHM+ F NSMSLKCA++LGI D +H+H  HPM LS
Sbjct: 5   NMDIS------LSTEQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKH-DHPMTLS 64

Query: 62  LLVSALQLHPNKTPFLSRLMRILTHSGFFVEKELEG----YMLTNSSRLLLKDNPLSVTP 121
            L+ A+ ++  K+    RLMR L +S FF+E+        Y LT +SRLLLK  PL+V P
Sbjct: 65  QLLKAIPINKEKSQSFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAPLTVAP 124

Query: 122 FLLSMLDSALVEPWQFLSVWFQSDG-PTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDA 181
            +  +LD     PW ++S WF+ +   T F+ A+G  FWE + NKP+ G  F+  ++ D+
Sbjct: 125 LVQVVLDPTFTNPWHYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDS 184

Query: 182 RLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKG 241
           RLV  VL   YK V DG+ +LVDVGGG GT+AKAI EA P ++CTV DLP VV  L+   
Sbjct: 185 RLVAHVLTKDYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTD 244

Query: 242 KFKYVEGDMFKAIPPADSILLK 259
           K  Y+ GDMF++IP AD+ILLK
Sbjct: 245 KLSYIGGDMFQSIPSADAILLK 259

[Close]

BLAST of Spg006327 vs. ExPASy TrEMBL
Match: A0A6J1GK49 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455045 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.7e-117
Identity = 211/268 (78.73%), Postives = 233/268 (86.94%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+LEGGKMA +AG+ELLEAQAHIWNH+FNFINSM+LKCA++LGI DAIHRHGPHPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLSR 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV------------EKELEGYMLTNSSRLLLKD 122
           LVSALQLHPNKT F+ RLMR+LTHSGFFV            E+E EGY+LTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLKH 120

Query: 123 NPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNA 182
           NP +VTPFLLSML  ALVEPWQ LS WFQS+  TPF TAHGMPFWEF+GNK  DG+ FNA
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNA 180

Query: 183 GIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVG 242
           G+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTV KAIAEAFP+IECTVFDLPQVVG
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQVVG 240

Query: 243 DLKGKGKFKYVEGDMFKAIPPADSILLK 259
           +LKG+G  KYVEGDMFKAIPPAD++LLK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADALLLK 268

[Close]

BLAST of Spg006327 vs. ExPASy TrEMBL
Match: A0A6J1HZ45 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468224 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.8e-114
Identity = 207/268 (77.24%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+LEGGKMA +AG+ELLEAQAHIWNH+FNFINSM+LKCA++LGI DAIHRH PHPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLSR 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV------------EKELEGYMLTNSSRLLLKD 122
           LVSALQLHPNKT F+ RLMR+LTHSGFFV            E+E EGY+LTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLKH 120

Query: 123 NPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNA 182
           NP   TPFLLSML  ALVEPWQ LS+WFQS+  TPF TAHGMP WEF+GNK  DG+ FNA
Sbjct: 121 NPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFNA 180

Query: 183 GIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVG 242
           G+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTVAKAIAEAFP+IECTV DLPQVVG
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQVVG 240

Query: 243 DLKGKGKFKYVEGDMFKAIPPADSILLK 259
           +LKG+G  KYVEGDMFKAIP AD++LLK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPSADALLLK 268

[Close]

BLAST of Spg006327 vs. ExPASy TrEMBL
Match: A0A6J1GKA3 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455047 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.0e-113
Identity = 205/268 (76.49%), Postives = 228/268 (85.07%), Query Frame = 0


Query: 3   MDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLSL 62
           M+ EG KM ++AGDE+LEAQAHIWNH+FNFINSM+LKCAVELGI D IHRH PHPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 63  LVSALQLHPNKTPFLSRLMRILTHSGFFV------------EKELEGYMLTNSSRLLLKD 122
           LVSALQLH NKT F+ RLMR+LTHSGFFV            E+E +GY+LTNSSRLLLK 
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKH 120

Query: 123 NPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNA 182
           NP +VTPFLLSML  ALVEPWQ LS+WFQS+  TPF TAHGMPFWEF+GNK  DG+ FN 
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNE 180

Query: 183 GIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVG 242
           G+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTVAKAIAEAFP+IECTVFDLPQVVG
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQVVG 240

Query: 243 DLKGKGKFKYVEGDMFKAIPPADSILLK 259
           +LKG+G  KYVEGDMFKAIPPAD ++LK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADVLVLK 268

[Close]

BLAST of Spg006327 vs. ExPASy TrEMBL
Match: A0A6J1GJY8 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455044 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.7e-110
Identity = 202/269 (75.09%), Postives = 226/269 (84.01%), Query Frame = 0


Query: 1   MNMDLEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPL 60
           MN + E   + +VAGDELLEAQAHIWNH+ NFINSM+LKCAVELGI DAIHRHGPHPMPL
Sbjct: 5   MNFESE-NMLPIVAGDELLEAQAHIWNHLLNFINSMTLKCAVELGIPDAIHRHGPHPMPL 64

Query: 61  SLLVSALQLHPNKTPFLSRLMRILTHSGFFV-----------EKELEGYMLTNSSRLLLK 120
           S LVSALQLHP+KT F+ RLMR+LTHSGFFV           E+E EGY+LTNSSRLLLK
Sbjct: 65  SRLVSALQLHPHKTRFIYRLMRLLTHSGFFVLQKLPKNSGEEEEEEEGYVLTNSSRLLLK 124

Query: 121 DNPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFN 180
            NP  VTP LLS+L  ALVEPW  LSVWF+S+  TPFDTAHG+ +WEFMG+KP  GD+FN
Sbjct: 125 YNPCGVTPLLLSILQPALVEPWHLLSVWFKSEDRTPFDTAHGISYWEFMGSKPKHGDIFN 184

Query: 181 AGIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVV 240
           AG+ASDARLVMSVLL K+KGVFDGV SLVDVGGGTGTV KAIAEAFP+IEC VFDLPQVV
Sbjct: 185 AGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECAVFDLPQVV 244

Query: 241 GDLKGKGKFKYVEGDMFKAIPPADSILLK 259
           G+LKG+G  KYVEGDMFKAIPPAD ++LK
Sbjct: 245 GELKGEGNLKYVEGDMFKAIPPADVLVLK 272

[Close]

BLAST of Spg006327 vs. ExPASy TrEMBL
Match: A0A6J1I050 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111468226 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.6e-110
Identity = 200/265 (75.47%), Postives = 226/265 (85.28%), Query Frame = 0


Query: 3   MDLEGGKM-AVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGPHPMPLS 62
           M++EG KM  +VAG+ELLEAQAHIWNH+ NFINSM+LKCAVELGI DAIH H P PMPLS
Sbjct: 1   MNIEGEKMLPIVAGEELLEAQAHIWNHLLNFINSMTLKCAVELGIPDAIHHHAPRPMPLS 60

Query: 63  LLVSALQLHPNKTPFLSRLMRILTHSGFFVEKEL--------EGYMLTNSSRLLLKDNPL 122
            LVSALQLHPNKT F+ RLMR+LTHSGFFV ++L        EGY+LTNSSRLLLK NP 
Sbjct: 61  RLVSALQLHPNKTHFIYRLMRLLTHSGFFVLQKLPKNSGEEEEGYVLTNSSRLLLKHNPC 120

Query: 123 SVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIA 182
            V PFLLS+L  ALVEPW  LSVWF+S   TPFD AHGM FWEFMG+KP DGD+FN+G+A
Sbjct: 121 GVAPFLLSLLQPALVEPWHLLSVWFKSKDRTPFDMAHGMSFWEFMGSKPKDGDIFNSGMA 180

Query: 183 SDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLK 242
           SDARLVMS+LL K+KGVFDGV SLVD+GGGTGTVAKAIAEAFP+IECTVFDLPQVVG+LK
Sbjct: 181 SDARLVMSLLLDKHKGVFDGVASLVDIGGGTGTVAKAIAEAFPQIECTVFDLPQVVGELK 240

Query: 243 GKGKFKYVEGDMFKAIPPADSILLK 259
           G+G  +YVEGDMFKAIPPAD ++LK
Sbjct: 241 GEGNLRYVEGDMFKAIPPADVLVLK 265

[Close]

BLAST of Spg006327 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.3e-36
Identity = 97/266 (36.47%), Postives = 138/266 (51.88%), Query Frame = 0


Query: 5   LEGGKMAVVAGDELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRH-GPHPMPLSLL 64
           +E  K  V+  DE  +A   IW ++F F +  + KCA++L I +AI  H    P+ L+ L
Sbjct: 15  MEDNKRKVL--DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAEL 74

Query: 65  VSALQLHPNKTPFLSRLMRILTHSGFFVEKELE-----GYMLTNSSRLLL--KDNPLSVT 124
            SA+   P+    L R+MR L H G F E   +     GY+ T  SR L+  + +  S+ 
Sbjct: 75  SSAVSASPS---HLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLA 134

Query: 125 PFLLSMLDSALVEPWQFLSVWFQS----DGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGI 184
           PF+L      ++ PW  LS    S      P PFD  HG   W F  + P   D+ N  +
Sbjct: 135 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 194

Query: 185 ASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDL 244
           A DAR V+  + G   G+FDGV ++VDVGGGTG     + + FP I+   FDLP V+   
Sbjct: 195 ACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVA 254

Query: 245 KGKGKFKYVEGDMFKAIPPADSILLK 259
           +     + VEGDMF +IP  D+I +K
Sbjct: 255 EVLDGVENVEGDMFDSIPACDAIFIK 275

[Close]

BLAST of Spg006327 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 8.2e-27
Identity = 78/244 (31.97%), Postives = 116/244 (47.54%), Query Frame = 0


Query: 16  DELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRH-GPHPMPLSLLVSALQLHPNKT 75
           DE  +A   IW ++F F +  + KCA++L I +AI  H    P+ LS L SA+   P+  
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPS-- 69

Query: 76  PFLSRLMRILTHSGFFVEKELEGYMLTNSSRLLLKDNPLSVTPFLLSMLDSALVEPWQFL 135
             L R+MR L H G F E   +  + T  +           TP    M+ + L       
Sbjct: 70  -HLRRIMRFLVHQGLFKEVPTKDGLATGYTN----------TPLSRRMMITKL------- 129

Query: 136 SVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDARLVMSVLLGKYKGVFDGV 195
                          HG   W F  +      + N  +A DAR V+  + G  +G+FDGV
Sbjct: 130 ---------------HGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDGV 189

Query: 196 GSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKGKFKYVEGDMFKAIPPADS 255
            ++VDVGGGTG     + + FP I+   FDLP V+   +     + VEGDMF +IP +D+
Sbjct: 190 ATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDA 218

Query: 256 ILLK 259
           +++K
Sbjct: 250 VIIK 218

[Close]

BLAST of Spg006327 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-26
Identity = 75/232 (32.33%), Postives = 117/232 (50.43%), Query Frame = 0


Query: 36  MSLKCAVELGIADAIHRHGPHPMPLSLLVSALQLHPNKTPFLSRLMRILTHSGFFV--EK 95
           M+LK A+EL + + + ++G    P  +       +P     L R++R+LT         +
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 96  ELEG------YMLTNSSRLLLK-DNPLSVTPFLLSMLDSALVEPWQFLSVWFQSDGPTPF 155
           +L G      Y L    + L K ++ +S+    L   D  L+E W  L      DG  PF
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPF 153

Query: 156 DTAHGMPFWEFMGNKPNDGDVFNAGIASDARLVMSVLLGKYKGVFDGVGSLVDVGGGTGT 215
           + A+GM  +E+ G  P    VFN G+++ + + M  +L  YKG F+G+ SLVDVGGG G 
Sbjct: 154 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGA 213

Query: 216 VAKAIAEAFPEIECTVFDLPQVVGDLKGKGKFKYVEGDMFKAIPPADSILLK 259
             K I   +P ++   FDLP V+ D       ++V GDMF ++P  D+I +K
Sbjct: 214 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMK 263

[Close]

BLAST of Spg006327 vs. TAIR 10
Match: AT1G63140.1 (O-methyltransferase family protein )

HSP 1 Score: 98.6 bits (244), Expect = 8.8e-21
Identity = 73/263 (27.76%), Postives = 118/263 (44.87%), Query Frame = 0


Query: 16  DELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGP----HPMPLSLLVSALQLHP 75
           ++ +  QA    H   F   M LK A+ELG+ D I          P  ++L +     +P
Sbjct: 26  EDTVSLQAERILHAMTF--PMVLKTALELGVIDMITSVDDGVWLSPSEIALGLPTKPTNP 85

Query: 76  NKTPFLSRLMRILTHSGFFVEKELEGYMLTNSSR--------------LLLKDNPLSVTP 135
                L R++ +L        + +E      S +              L   D   S+  
Sbjct: 86  EAPVLLDRMLVLLASHSILKYRTVETGDNIGSRKTERVYAAEPVCTFFLNRGDGLGSLAT 145

Query: 136 FLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDAR 195
             + +     ++PW+ L      +G   F +AHGM F+E +G+     ++FN  ++  + 
Sbjct: 146 LFMVLQGEVCMKPWEHLKDMI-LEGKDAFTSAHGMRFFELIGSNEQFAEMFNRAMSEAST 205

Query: 196 LVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKGK 255
           L+M  +L  YKG F+ V +LVDVGGG GT+   +   +P I+   FDL  V+        
Sbjct: 206 LIMKKVLEVYKG-FEDVNTLVDVGGGIGTIIGQVTSKYPHIKGINFDLASVLAHAPFNKG 265

Query: 256 FKYVEGDMFKAIPPADSILLKVN 261
            ++V GDMFK IP  D+I +KV+
Sbjct: 266 VEHVSGDMFKEIPKGDAIFMKVS 284

[Close]

BLAST of Spg006327 vs. TAIR 10
Match: AT1G63140.2 (O-methyltransferase family protein )

HSP 1 Score: 95.9 bits (237), Expect = 5.7e-20
Identity = 77/282 (27.30%), Postives = 124/282 (43.97%), Query Frame = 0


Query: 16  DELLEAQAHIWNHMFNFINSMSLKCAVELGIADAIHRHGP----HPMPLSLLVSALQLHP 75
           ++ +  QA    H   F   M LK A+ELG+ D I          P  ++L +     +P
Sbjct: 26  EDTVSLQAERILHAMTF--PMVLKTALELGVIDMITSVDDGVWLSPSEIALGLPTKPTNP 85

Query: 76  NKTPFLSRLMRILTHSGFFVEKELEGYMLTNSSR--------------LLLKDNPLSVTP 135
                L R++ +L        + +E      S +              L   D   S+  
Sbjct: 86  EAPVLLDRMLVLLASHSILKYRTVETGDNIGSRKTERVYAAEPVCTFFLNRGDGLGSLAT 145

Query: 136 FLLSMLDSALVEPWQFLSVWFQSDGPTPFDTAHGMPFWEFMGNKPNDGDVFNAGIASDAR 195
             + +     ++PW+ L      +G   F +AHGM F+E +G+     ++FN  ++  + 
Sbjct: 146 LFMVLQGEVCMKPWEHLKDMI-LEGKDAFTSAHGMRFFELIGSNEQFAEMFNRAMSEAST 205

Query: 196 LVMSVLLGKYKGVFDGVGSLVDVGGGTGTVAKAIAEAFPEIECTVFDLPQVVGDLKGKGK 255
           L+M  +L  YKG F+ V +LVDVGGG GT+   +   +P I+   FDL  V+        
Sbjct: 206 LIMKKVLEVYKG-FEDVNTLVDVGGGIGTIIGQVTSKYPHIKGINFDLASVLAHAPFNKG 265

Query: 256 FKYVEGDMFKAIPPADSILLKVNIIFYCYPYMSRVHILQQIW 280
            ++V GDMFK IP  D+I +K   I + +     V IL+  W
Sbjct: 266 VEHVSGDMFKEIPKGDAIFMK--WILHDWTDEDCVKILKNYW 301

The following BLAST results are available for this feature:

BLAST of Spg006327 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952337.1	3.6e-117	78.73	trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata]	[more]
KAG6572268.1	2.8e-114	77.74	putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022969140.1	3.7e-114	77.24	trans-resveratrol di-O-methyltransferase-like [Cucurbita maxima]	[more]
XP_022952338.1	4.1e-113	76.49	probable O-methyltransferase 3 [Cucurbita moschata]	[more]
XP_023554524.1	9.1e-113	76.69	probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg006327 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	5.3e-79	58.73	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	4.5e-78	57.25	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
A0A0N9HMN6	4.8e-72	50.94	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
Q6VMW0	1.1e-65	51.76	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
Q93WU3	1.5e-65	48.47	Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1	[more]

BLAST of Spg006327 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GK49	1.7e-117	78.73	trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HZ45	1.8e-114	77.24	trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1GKA3	2.0e-113	76.49	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455047 PE=...	[more]
A0A6J1GJY8	2.7e-110	75.09	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455044 PE=...	[more]
A0A6J1I050	4.6e-110	75.47	probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111468226 PE=3 ...	[more]

BLAST of Spg006327 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	4.3e-36	36.47	O-methyltransferase family protein	[more]
AT4G35150.1	8.2e-27	31.97	O-methyltransferase family protein	[more]
AT5G54160.1	1.4e-26	32.33	O-methyltransferase 1	[more]
AT1G63140.1	8.8e-21	27.76	O-methyltransferase family protein	[more]
AT1G63140.2	5.7e-20	27.30	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 36..85 e-value: 9.0E-16 score: 57.6
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 10..107 e-value: 4.7E-38 score: 130.9
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 108..281 e-value: 1.5E-44 score: 154.0
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 13..276
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 197..278 e-value: 0.00129157 score: 35.8687
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 131..259 e-value: 1.6E-30 score: 106.0
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 13..276
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 26..290 score: 46.135048
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 112..280
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 15..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg006327.1	Spg006327.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity