Spg006324 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006324
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
Locationscaffold4: 6293135 .. 6294555 (+)
RNA-Seq ExpressionSpg006324
SyntenySpg006324
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTGCCCCCATTCTCCCCTTCCGATCTCCTACTTTGGGCACCCCTCTTGGGGATAAACCCTCAAATGCTTCTTTTTGGTCCCCAATTATTGATAAAATTCAGCGTAAAGCTTGAAAATTGGCGTTTCTCATATATTTCGAAGGGCGGTCGTTTAACTCTTATTCAAGCAACTCTGAGTAGTATCTCAACGTACATGCTGTCAGTTTTTAAGGTCCCTCAGTCTATTTGTAAGAAGGTGGATAAGCTTATCAGAACTTTCCTTTGGTGTGGTACTAGCGTTGATAACAACCTCCGGCCTTTGATTAGTTGGGATGTGGTGACTGCCGCAAAAGACTCAGGGGGCTTTGATATTTTCAAAACCAAAGTTTCAAATAATGCCCTTCAAATCAAATGGTTGTGGAGGTACTATCAGGAGGATCAACCCTTATGGAAACGCCTTGTTAATGCCAAATATACTATCCCACAAGCTGGAGCTCTCCCAACGCAGTCCCGTTTCTCCAATTCTAGGGCCCCTTGGTTTTCTATTGTCAAGCATGCTCATCATTTCAAGCAGTTCACTAAATGGGGCCTCAGAAATGGAAATAAAATTTTCTTTTGGCAAGATTCTTGGTCAAATAATCTAGGCCCGTTAAACCTGCTTCATCCTCGTTTCTTTGCGTTTTCCAGTAGGCAGGATATGAAAGTCTTTGAGGCTTGGGTGTCTTCTTCATATTCTTGGAATATTTTCCCTCGTAGACCTCTTCTGGACAGAGAGGTTAGCCTTTGGGACGAGTTAATCCCCTCACTGCCCAGACCCTCTTCTTCTAGAGGGCCTTTCTCAACGGCTTTAGCTCACCTCTCTTTCTGGGATAGCTCTTCTATCATGCGCTTACAGGTGGACCTTAAAGTCCTCACAAACCTTTGGCGAGCCAGAATCCTTAAGAAACCTTTGGTGAGCCAGAATCCCTAAGAAGTGTAAATTTTTTATGTGGTTTATGCATCACCCAAGGTTACACACGGCAGATAGGTTGCAAAGAATTTTCAAGCAATCAACTTTGAGCCCCGGTTGTTGTTCCCTTTGTCATGTTGCCTCAGAAATCCTTGGATCATATTTTCGTTCATTGCCGGTTGGCCTCAACCATTTGGACTATAATTCTTCAAGCCTCTGGTGCTCGTTCTCCAATCCCTTCTCAAGTTGACAAGCTTTGTGAGGAGGCTTTTGCTTTCAAGGCTGGCTCTCAAAGGAGCTCTCTCATCCAAGGTCTGTTTGTGGCTTCTTTTTGGGTTATTTGGAATGAGCAGAATAGTCGTATCTTCAAAAATCAATCTCACTCTCCTTATCAGATTTGGGAGGATATCCTTGCTTTAGCGGTTTTTGGGACCTCTTCTTCTACGTTTTTTATCAACTATGATGCGCCTTCCATTGCCCTTAATTAG

mRNA sequence

ATGGGGTTGCCCCCATTCTCCCCTTCCGATCTCCTACTTTGGGCACCCCTCTTGGGGATAAACCCTCAAATGCTTCTTTTTGGTCCCCAATTATTGATAAAATTCAGCGTAAAGCTTGAAAATTGGCGTTTCTCATATATTTCGAAGGGCGGTCGTTTAACTCTTATTCAAGCAACTCTGAGTAGTATCTCAACGTACATGCTGTCAGTTTTTAAGGTCCCTCAGTCTATTTGTAAGAAGGTGGATAAGCTTATCAGAACTTTCCTTTGGTGTGGTACTAGCGTTGATAACAACCTCCGGCCTTTGATTAGTTGGGATGTGGTGACTGCCGCAAAAGACTCAGGGGGCTTTGATATTTTCAAAACCAAAGTTTCAAATAATGCCCTTCAAATCAAATGGTTGTGGAGGTACTATCAGGAGGATCAACCCTTATGGAAACGCCTTGTTAATGCCAAATATACTATCCCACAAGCTGGAGCTCTCCCAACGCAGTCCCGTTTCTCCAATTCTAGGGCCCCTTGGTTTTCTATTGTCAAGCATGCTCATCATTTCAAGCAGTTCACTAAATGGGGCCTCAGAAATGGAAATAAAATTTTCTTTTGGCAAGATTCTTGGTCAAATAATCTAGGCCCGTTAAACCTGCTTCATCCTCGTTTCTTTGCGTTTTCCAGTAGGCAGGATATGAAAGTCTTTGAGGCTTGGGTGTCTTCTTCATATTCTTGGAATATTTTCCCTCGTAGACCTCTTCTGGACAGAGAGGTTAGCCTTTGGGACGAGTTAATCCCCTCACTGCCCAGACCCTCTTCTTCTAGAGGGCCTTTCTCAACGGCTTTAGCTCACCTCTCTTTCTGGGATAGCTCTTCTATCATGCGCTTACAGGTGGACCTTAAAGTCCTCACAAACCTTTGGCGAGCCAGAATCCTTAAGAAACCTTTGAAATCCTTGGATCATATTTTCGTTCATTGCCGGTTGGCCTCAACCATTTGGACTATAATTCTTCAAGCCTCTGGTGCTCGTTCTCCAATCCCTTCTCAAGTTGACAAGCTTTGTGAGGAGGCTTTTGCTTTCAAGGCTGGCTCTCAAAGGAGCTCTCTCATCCAAGGTCTGTTTGTGGCTTCTTTTTGGGTTATTTGGAATGAGCAGAATAGTCGTATCTTCAAAAATCAATCTCACTCTCCTTATCAGATTTGGGAGGATATCCTTGCTTTAGCGGTTTTTGGGACCTCTTCTTCTACGTTTTTTATCAACTATGATGCGCCTTCCATTGCCCTTAATTAG

Coding sequence (CDS)

ATGGGGTTGCCCCCATTCTCCCCTTCCGATCTCCTACTTTGGGCACCCCTCTTGGGGATAAACCCTCAAATGCTTCTTTTTGGTCCCCAATTATTGATAAAATTCAGCGTAAAGCTTGAAAATTGGCGTTTCTCATATATTTCGAAGGGCGGTCGTTTAACTCTTATTCAAGCAACTCTGAGTAGTATCTCAACGTACATGCTGTCAGTTTTTAAGGTCCCTCAGTCTATTTGTAAGAAGGTGGATAAGCTTATCAGAACTTTCCTTTGGTGTGGTACTAGCGTTGATAACAACCTCCGGCCTTTGATTAGTTGGGATGTGGTGACTGCCGCAAAAGACTCAGGGGGCTTTGATATTTTCAAAACCAAAGTTTCAAATAATGCCCTTCAAATCAAATGGTTGTGGAGGTACTATCAGGAGGATCAACCCTTATGGAAACGCCTTGTTAATGCCAAATATACTATCCCACAAGCTGGAGCTCTCCCAACGCAGTCCCGTTTCTCCAATTCTAGGGCCCCTTGGTTTTCTATTGTCAAGCATGCTCATCATTTCAAGCAGTTCACTAAATGGGGCCTCAGAAATGGAAATAAAATTTTCTTTTGGCAAGATTCTTGGTCAAATAATCTAGGCCCGTTAAACCTGCTTCATCCTCGTTTCTTTGCGTTTTCCAGTAGGCAGGATATGAAAGTCTTTGAGGCTTGGGTGTCTTCTTCATATTCTTGGAATATTTTCCCTCGTAGACCTCTTCTGGACAGAGAGGTTAGCCTTTGGGACGAGTTAATCCCCTCACTGCCCAGACCCTCTTCTTCTAGAGGGCCTTTCTCAACGGCTTTAGCTCACCTCTCTTTCTGGGATAGCTCTTCTATCATGCGCTTACAGGTGGACCTTAAAGTCCTCACAAACCTTTGGCGAGCCAGAATCCTTAAGAAACCTTTGAAATCCTTGGATCATATTTTCGTTCATTGCCGGTTGGCCTCAACCATTTGGACTATAATTCTTCAAGCCTCTGGTGCTCGTTCTCCAATCCCTTCTCAAGTTGACAAGCTTTGTGAGGAGGCTTTTGCTTTCAAGGCTGGCTCTCAAAGGAGCTCTCTCATCCAAGGTCTGTTTGTGGCTTCTTTTTGGGTTATTTGGAATGAGCAGAATAGTCGTATCTTCAAAAATCAATCTCACTCTCCTTATCAGATTTGGGAGGATATCCTTGCTTTAGCGGTTTTTGGGACCTCTTCTTCTACGTTTTTTATCAACTATGATGCGCCTTCCATTGCCCTTAATTAG

Protein sequence

MGLPPFSPSDLLLWAPLLGINPQMLLFGPQLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKYTIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLNLLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSLPRPSSSRGPFSTALAHLSFWDSSSIMRLQVDLKVLTNLWRARILKKPLKSLDHIFVHCRLASTIWTIILQASGARSPIPSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWEDILALAVFGTSSSTFFINYDAPSIALN
Homology
BLAST of Spg006324 vs. NCBI nr
Match: KAA0040037.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 262.7 bits (670), Expect = 5.5e-66
Identity = 150/443 (33.86%), Postives = 227/443 (51.24%), Query Frame = 0

Query: 34  KFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGT 93
           K + KL +W++S +SKGG++TLI+++L+S+ TY LS+FK P S CK ++K  R FLW   
Sbjct: 101 KINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNP 160

Query: 94  SVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKY 153
              + L  L++W  +T+ K+ GG  I + K +N AL  KWLWRY  ED PLWK+++NAKY
Sbjct: 161 PETHKLH-LVNWAKITSPKERGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKY 220

Query: 154 TIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLN 213
                G +P     S+SR+PWFSI K    F++   W ++NG    FW   W  N  PL+
Sbjct: 221 RSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQN-SPLS 280

Query: 214 LLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSL--------- 273
             +PR FA S+ +D  + + W ++   W++ PRR L + E  LW EL  SL         
Sbjct: 281 FHYPRLFALSTNKDSSIRDMWNNTLMDWDLNPRRQLREWEYPLWAELKNSLNASFCENGK 340

Query: 274 PRPS---SSRGPFSTALAHLSFWD-SSSIMRLQVDLKVLTNLWRARILKKPL-------- 333
             P+   +S G ++ A    +      S++ LQ       NLW+  I KK +        
Sbjct: 341 DSPTWTLNSNGLYTVASVKKALQQPDQSLLDLQ-SQNTFKNLWKTSIPKKCIFFIWTLLY 400

Query: 334 ------------------------------KSLDHIFVHCRLASTIWTIILQASGARSPI 393
                                         +   H+F+ C +A +IW +I     +    
Sbjct: 401 DSVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRLISSHLNSNVNC 460

Query: 394 PSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWEDILA 426
            S  D LC    ++K  ++++ ++   + ++ W IW E+N+RIF  +  +  +IWEDI A
Sbjct: 461 LSPKD-LCITMCSWKQKTKKNVILYNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKA 520

BLAST of Spg006324 vs. NCBI nr
Match: TYK06777.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-64
Identity = 150/443 (33.86%), Postives = 231/443 (52.14%), Query Frame = 0

Query: 34   KFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGT 93
            K + KL +W++S +SKGG++TLI+++L+S+ TY LS+FK P S CK ++K  R FLW   
Sbjct: 677  KINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNP 736

Query: 94   SVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKY 153
               + L  L++W  +T++K+ GG  I + K +N AL  KWLWRY  ED PLWK+++NAKY
Sbjct: 737  PETHKLH-LVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKY 796

Query: 154  TIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLN 213
                 G +P     S+SR+PWFSI K    F++   W ++NG    FW   W  N  PL+
Sbjct: 797  RSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHGHWHQN-SPLS 856

Query: 214  LLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSLPRPSSSRGP 273
              +PR +A S+ ++  + + W ++   W++ PRR L + E+ LW EL  S+       G 
Sbjct: 857  SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWELPLWAELKNSINASFCENGK 916

Query: 274  FST--ALAHLSFWDSSSIMR-LQ------VDLK---VLTNLWRARILKKPL--------- 333
             S   AL     +  +S+ + LQ      +DL+      NLW+  I KK +         
Sbjct: 917  DSPMWALNSNGLYTVASVKKALQQPEQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYD 976

Query: 334  -----------------------------KSLDHIFVHCRLASTIWTIILQASGARSPIP 393
                                         +   H+F+ C +A +IW  I  +S   S + 
Sbjct: 977  SVNTAEQLMKRLPKLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRTI--SSHLNSNVN 1036

Query: 394  SQVDK-LCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWEDILA 426
                K LC    ++K  ++++ ++   + ++ W IW E+N+RIF  +  +  +IWEDI A
Sbjct: 1037 CLSPKELCITMCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKA 1096

BLAST of Spg006324 vs. NCBI nr
Match: KAA0041397.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 257.3 bits (656), Expect = 2.3e-64
Identity = 150/457 (32.82%), Postives = 221/457 (48.36%), Query Frame = 0

Query: 18   LGINPQMLLFGPQLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSI 77
            LG  P    F   +L K   KL +W++S +SKGGR+TLI +TL S+  Y LSVFKVP+ I
Sbjct: 578  LGGKPSSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGI 637

Query: 78   CKKVDKLIRTFLWCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRY 137
             +K++   R FLW GTS  +N+  LI W+ V + K+ GG  I     +N AL  KWLW++
Sbjct: 638  AQKIEAYWRNFLWNGTSNGHNI-SLIRWNQVVSPKEKGGLGIHSVHSTNFALLCKWLWKF 697

Query: 138  YQEDQPLWKRLVNAKYTIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNK 197
              E +PLWKRL+ +KY   + G  P++ ++S++ +PW ++      F +   W + +G  
Sbjct: 698  LTEKEPLWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGED 757

Query: 198  IFFWQDSWSNNLGPLNLLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLW 257
            I FW D+W+ N  PL+L+ PR FA S+ +   V + W  S   WNI   RPL D E +LW
Sbjct: 758  ISFWLDNWNGN-SPLSLVVPRLFALSTNKKGSVKDLWNPSLKDWNIHVNRPLRDHEKNLW 817

Query: 258  DELIPSLPRPSSSRGPFST--ALAHLSFWDSSSIMR---------LQVDLKVLTNLWRAR 317
              +  SLP P   RGP      L   + +D++SI +               +   LW+  
Sbjct: 818  HNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPTNFHPSLYKTLWKVD 877

Query: 318  ILK--------------------------------------KPLKSLDHIFVHCRLASTI 377
              K                                      K  + ++H+F+HC  +  +
Sbjct: 878  FPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQL 937

Query: 378  WTIILQASGARSPIPSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKN 426
            W+   QA    +  P+ V  L +   +    +Q+  +         W IW E+N+RIFK 
Sbjct: 938  WS-KAQALLKWNSTPNDVKSLAQNICSLNIKTQKGLITFNTIAILLWKIWLERNNRIFKQ 997

BLAST of Spg006324 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 256.1 bits (653), Expect = 5.2e-64
Identity = 150/447 (33.56%), Postives = 230/447 (51.45%), Query Frame = 0

Query: 34   KFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGT 93
            K + KL +W++S +SKGG++TLI+++L+S+ TY LS+FKVP S CK ++K  R FLW   
Sbjct: 614  KINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNP 673

Query: 94   SVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKY 153
               + L  L++W  +T++K+ GG  I + K +N AL  KWLWRY  ED PLWK+++NAKY
Sbjct: 674  PETHKLH-LVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKY 733

Query: 154  TIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLN 213
                 G +P     S+SR+PWFSI K    F++   W ++NG    FW   W  N  PL+
Sbjct: 734  RSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQN-SPLS 793

Query: 214  LLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSLPRPSSSRG- 273
              +PR +A S+ ++  + + W ++   W++ PRR L + E  LW EL  SL       G 
Sbjct: 794  SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWEHPLWAELKNSLNASFCENGN 853

Query: 274  --PFSTALAHLSFWDSSSIMRLQ------VDLK---VLTNLWRARILKKPL--------- 333
              P  T  ++  +  +S    LQ      +D +      NLW+  I KK +         
Sbjct: 854  DSPMWTLNSNGLYTVASVKKTLQQPEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWTLLYD 913

Query: 334  -----------------------------KSLDHIFVHCRLASTIWTIILQASGARSPIP 393
                                         +   H+F+ C +A +IW  I       S + 
Sbjct: 914  SVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRSI------SSHLS 973

Query: 394  SQVD-----KLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWE 426
            S V+     +LC    ++K  ++++ ++   + ++ W IW E+N+RIF  +  +  +IWE
Sbjct: 974  SNVNCLSPKELCITMCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWE 1033

BLAST of Spg006324 vs. NCBI nr
Match: TYK00493.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 255.8 bits (652), Expect = 6.7e-64
Identity = 158/456 (34.65%), Postives = 230/456 (50.44%), Query Frame = 0

Query: 18   LGINPQMLLFGPQLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSI 77
            LG NP+  LF   +  +   KL NW++++ISKGGRLTLI++TLSS+  Y LSVF+ P S 
Sbjct: 1329 LGGNPKSNLFWRNIEDRIQKKLSNWKYAHISKGGRLTLIKSTLSSLPIYKLSVFQAPSST 1388

Query: 78   CKKVDKLIRTFLWCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRY 137
             K ++KL R FLW G S       LI+W +VT  K+ GG  I + +V+N AL  KWLWRY
Sbjct: 1389 YKNIEKLWRNFLWKG-SCGLKGSHLINWSIVTKPKEEGGLGISRLQVTNQALLSKWLWRY 1448

Query: 138  YQEDQPLWKRLVNAKYTIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNK 197
            Y E   LW+RL++ KY     G LP+    S+S+APW SI+ +   FK    W L NG++
Sbjct: 1449 YSEPNSLWRRLIHIKYKGKHPGDLPSNISSSSSKAPWRSIINNIDWFKSNQGWDLNNGDQ 1508

Query: 198  IFFWQDSWSNNLGPLNLLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLW 257
            I FW  +WS   G L+  +PR FA S  ++  + + W S++  W I  RR L DRE+S W
Sbjct: 1509 ISFWYSNWSPE-GCLSTAYPRLFALSMDKESSIKDVWNSNNNQWEITFRRKLNDRELSTW 1568

Query: 258  DELIPSLPRPSSSRGP------------FSTALAH--LSFWDSSSIMRLQVDLKVLTNLW 317
             +++ +LP   ++RGP            FS A A   +S     S+   +V  K+L  +W
Sbjct: 1569 QKILENLPILRTNRGPSKPTWIPDSKKFFSIASAKSCISHQPDRSVANPRV--KLLNLIW 1628

Query: 318  RARI--------------------------------LKKPLKSLDHIFVHCRLASTIWTI 377
            +  +                                 KK  ++  H+F+HC L   +W+ 
Sbjct: 1629 KTHVPMKIKFFMWCLVQRKLNTMEVXTLLQPNWCVLCKKKSETGAHLFLHCDLVKPLWS- 1688

Query: 378  ILQASGARSPIPSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSH 426
            +L  S   +PI    + +     +      +  ++    +A  W IW E+N+RIF   S+
Sbjct: 1689 LLHRSLNFAPISDDFEAMFSFFLSLNQSLPKHKVVFCGMIAILWRIWTERNNRIFDTLSY 1748

BLAST of Spg006324 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.1e-19
Identity = 59/187 (31.55%), Postives = 89/187 (47.59%), Query Frame = 0

Query: 30  QLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFL 89
           ++L + S ++  WR   +S  GRLTL +A LSS+  + +S   +PQSI  ++D+L RTFL
Sbjct: 15  EILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFL 74

Query: 90  WCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLV 149
           W G++ +   + L+ W  V + K  GG  +   K  N AL  K  WR  QE   LW  ++
Sbjct: 75  W-GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVL 134

Query: 150 NAKYTIPQAGALPTQSRFSNSRAPWFS-----------IVKHAHHFKQFTKWGLRNGNKI 206
             KY + +       SR+   +  W S           +V H         W   +G +I
Sbjct: 135 QKKYHVGEI----RDSRWLIPKGSWSSTWRSIAIGLRDVVSHG------VGWIPGDGQQI 190

BLAST of Spg006324 vs. ExPASy TrEMBL
Match: A0A5A7TES3 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00040 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 2.7e-66
Identity = 150/443 (33.86%), Postives = 227/443 (51.24%), Query Frame = 0

Query: 34  KFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGT 93
           K + KL +W++S +SKGG++TLI+++L+S+ TY LS+FK P S CK ++K  R FLW   
Sbjct: 101 KINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNP 160

Query: 94  SVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKY 153
              + L  L++W  +T+ K+ GG  I + K +N AL  KWLWRY  ED PLWK+++NAKY
Sbjct: 161 PETHKLH-LVNWAKITSPKERGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKY 220

Query: 154 TIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLN 213
                G +P     S+SR+PWFSI K    F++   W ++NG    FW   W  N  PL+
Sbjct: 221 RSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQN-SPLS 280

Query: 214 LLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSL--------- 273
             +PR FA S+ +D  + + W ++   W++ PRR L + E  LW EL  SL         
Sbjct: 281 FHYPRLFALSTNKDSSIRDMWNNTLMDWDLNPRRQLREWEYPLWAELKNSLNASFCENGK 340

Query: 274 PRPS---SSRGPFSTALAHLSFWD-SSSIMRLQVDLKVLTNLWRARILKKPL-------- 333
             P+   +S G ++ A    +      S++ LQ       NLW+  I KK +        
Sbjct: 341 DSPTWTLNSNGLYTVASVKKALQQPDQSLLDLQ-SQNTFKNLWKTSIPKKCIFFIWTLLY 400

Query: 334 ------------------------------KSLDHIFVHCRLASTIWTIILQASGARSPI 393
                                         +   H+F+ C +A +IW +I     +    
Sbjct: 401 DSVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRLISSHLNSNVNC 460

Query: 394 PSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWEDILA 426
            S  D LC    ++K  ++++ ++   + ++ W IW E+N+RIF  +  +  +IWEDI A
Sbjct: 461 LSPKD-LCITMCSWKQKTKKNVILYNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKA 520

BLAST of Spg006324 vs. ExPASy TrEMBL
Match: A0A5D3C4J1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00790 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 8.6e-65
Identity = 150/443 (33.86%), Postives = 231/443 (52.14%), Query Frame = 0

Query: 34   KFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGT 93
            K + KL +W++S +SKGG++TLI+++L+S+ TY LS+FK P S CK ++K  R FLW   
Sbjct: 677  KINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNP 736

Query: 94   SVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKY 153
               + L  L++W  +T++K+ GG  I + K +N AL  KWLWRY  ED PLWK+++NAKY
Sbjct: 737  PETHKLH-LVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKY 796

Query: 154  TIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLN 213
                 G +P     S+SR+PWFSI K    F++   W ++NG    FW   W  N  PL+
Sbjct: 797  RSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHGHWHQN-SPLS 856

Query: 214  LLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSLPRPSSSRGP 273
              +PR +A S+ ++  + + W ++   W++ PRR L + E+ LW EL  S+       G 
Sbjct: 857  SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWELPLWAELKNSINASFCENGK 916

Query: 274  FST--ALAHLSFWDSSSIMR-LQ------VDLK---VLTNLWRARILKKPL--------- 333
             S   AL     +  +S+ + LQ      +DL+      NLW+  I KK +         
Sbjct: 917  DSPMWALNSNGLYTVASVKKALQQPEQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYD 976

Query: 334  -----------------------------KSLDHIFVHCRLASTIWTIILQASGARSPIP 393
                                         +   H+F+ C +A +IW  I  +S   S + 
Sbjct: 977  SVNTAEQLMKRLPKLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRTI--SSHLNSNVN 1036

Query: 394  SQVDK-LCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWEDILA 426
                K LC    ++K  ++++ ++   + ++ W IW E+N+RIF  +  +  +IWEDI A
Sbjct: 1037 CLSPKELCITMCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWEDIKA 1096

BLAST of Spg006324 vs. ExPASy TrEMBL
Match: A0A5A7TIB8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00430 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.1e-64
Identity = 150/457 (32.82%), Postives = 221/457 (48.36%), Query Frame = 0

Query: 18   LGINPQMLLFGPQLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSI 77
            LG  P    F   +L K   KL +W++S +SKGGR+TLI +TL S+  Y LSVFKVP+ I
Sbjct: 578  LGGKPSSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGI 637

Query: 78   CKKVDKLIRTFLWCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRY 137
             +K++   R FLW GTS  +N+  LI W+ V + K+ GG  I     +N AL  KWLW++
Sbjct: 638  AQKIEAYWRNFLWNGTSNGHNI-SLIRWNQVVSPKEKGGLGIHSVHSTNFALLCKWLWKF 697

Query: 138  YQEDQPLWKRLVNAKYTIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNK 197
              E +PLWKRL+ +KY   + G  P++ ++S++ +PW ++      F +   W + +G  
Sbjct: 698  LTEKEPLWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGED 757

Query: 198  IFFWQDSWSNNLGPLNLLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLW 257
            I FW D+W+ N  PL+L+ PR FA S+ +   V + W  S   WNI   RPL D E +LW
Sbjct: 758  ISFWLDNWNGN-SPLSLVVPRLFALSTNKKGSVKDLWNPSLKDWNIHVNRPLRDHEKNLW 817

Query: 258  DELIPSLPRPSSSRGPFST--ALAHLSFWDSSSIMR---------LQVDLKVLTNLWRAR 317
              +  SLP P   RGP      L   + +D++SI +               +   LW+  
Sbjct: 818  HNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPTNFHPSLYKTLWKVD 877

Query: 318  ILK--------------------------------------KPLKSLDHIFVHCRLASTI 377
              K                                      K  + ++H+F+HC  +  +
Sbjct: 878  FPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQL 937

Query: 378  WTIILQASGARSPIPSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKN 426
            W+   QA    +  P+ V  L +   +    +Q+  +         W IW E+N+RIFK 
Sbjct: 938  WS-KAQALLKWNSTPNDVKSLAQNICSLNIKTQKGLITFNTIAILLWKIWLERNNRIFKQ 997

BLAST of Spg006324 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 2.5e-64
Identity = 150/447 (33.56%), Postives = 230/447 (51.45%), Query Frame = 0

Query: 34   KFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSICKKVDKLIRTFLWCGT 93
            K + KL +W++S +SKGG++TLI+++L+S+ TY LS+FKVP S CK ++K  R FLW   
Sbjct: 614  KINKKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNP 673

Query: 94   SVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRYYQEDQPLWKRLVNAKY 153
               + L  L++W  +T++K+ GG  I + K +N AL  KWLWRY  ED PLWK+++NAKY
Sbjct: 674  PETHKLH-LVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKY 733

Query: 154  TIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNKIFFWQDSWSNNLGPLN 213
                 G +P     S+SR+PWFSI K    F++   W ++NG    FW   W  N  PL+
Sbjct: 734  RSLSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQN-SPLS 793

Query: 214  LLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLWDELIPSLPRPSSSRG- 273
              +PR +A S+ ++  + + W ++   W++ PRR L + E  LW EL  SL       G 
Sbjct: 794  SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWEHPLWAELKNSLNASFCENGN 853

Query: 274  --PFSTALAHLSFWDSSSIMRLQ------VDLK---VLTNLWRARILKKPL--------- 333
              P  T  ++  +  +S    LQ      +D +      NLW+  I KK +         
Sbjct: 854  DSPMWTLNSNGLYTVASVKKTLQQPEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWTLLYD 913

Query: 334  -----------------------------KSLDHIFVHCRLASTIWTIILQASGARSPIP 393
                                         +   H+F+ C +A +IW  I       S + 
Sbjct: 914  SVNTAEQLMKRLPNLCSRPSWCVMCKRNDEDRIHLFILCPIAKSIWRSI------SSHLS 973

Query: 394  SQVD-----KLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKNQSHSPYQIWE 426
            S V+     +LC    ++K  ++++ ++   + ++ W IW E+N+RIF  +  +  +IWE
Sbjct: 974  SNVNCLSPKELCITMCSWKQKTKKNVILFNTYASALWNIWLERNARIFNGKEKTVAEIWE 1033

BLAST of Spg006324 vs. ExPASy TrEMBL
Match: A0A5A7T9I7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00980 PE=4 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 3.3e-64
Identity = 147/457 (32.17%), Postives = 220/457 (48.14%), Query Frame = 0

Query: 18   LGINPQMLLFGPQLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFKVPQSI 77
            LG  P    F   +L K   KL NW++S +SKGGR+TLI +TL S+  Y +SVFKVP+ I
Sbjct: 686  LGGRPSSSNFWDNVLQKIQKKLSNWKYSQLSKGGRITLINSTLESLPIYQMSVFKVPKGI 745

Query: 78   CKKVDKLIRTFLWCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIKWLWRY 137
             +K++   R FLW G S  +N+  LI W+ + + K+ GG  I     +N AL  KWLW++
Sbjct: 746  AQKIEASWRNFLWNGASNGHNI-SLIRWNQIVSPKEKGGLGIHSVNSTNFALLCKWLWKF 805

Query: 138  YQEDQPLWKRLVNAKYTIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGLRNGNK 197
              E  PLWKRL+ +KY   + G+ P+  +FS++ +PW ++ +    F +   W + +G  
Sbjct: 806  LTEKDPLWKRLIISKYDKEKMGSFPSHGKFSSNNSPWKAVTECISWFYKNISWKVNDGED 865

Query: 198  IFFWQDSWSNNLGPLNLLHPRFFAFSSRQDMKVFEAWVSSSYSWNIFPRRPLLDREVSLW 257
            I FW D+W+ N  PL+L  PR FA S+ +   V E W  SS  W++   RPL D E +LW
Sbjct: 866  ISFWLDNWNGN-APLSLAVPRLFALSTNKKGSVKEFWNPSSNDWHLHINRPLRDHEENLW 925

Query: 258  DELIPSLPRPSSSRGPFSTA--LAHLSFWDSSSIMRLQVDLKV---------LTNLWRAR 317
              +  SLP P  +RG       L   + +D++S+ R   +  +            LW+  
Sbjct: 926  HNIKASLPTPLPNRGHPKPLWNLNSNNIFDTASVKRAIAEAPISPANFHPNLYKTLWKVE 985

Query: 318  ILK--------------------------------------KPLKSLDHIFVHCRLASTI 377
              K                                      K  + ++H+F+HC  +  +
Sbjct: 986  FPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQEDINHLFIHCPYSQQL 1045

Query: 378  WTIILQASGARSPIPSQVDKLCEEAFAFKAGSQRSSLIQGLFVASFWVIWNEQNSRIFKN 426
            W+         S  P+ V  L +   +    +Q+  +         W IW E+N+RIFK 
Sbjct: 1046 WSKAKALLNWNS-TPTDVQSLIQNICSLNIRNQKGLITFNTNATILWKIWLERNNRIFKQ 1105

BLAST of Spg006324 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 75.9 bits (185), Expect = 8.9e-14
Identity = 51/194 (26.29%), Postives = 82/194 (42.27%), Query Frame = 0

Query: 13  LWAPLLGINPQMLLFGPQLLIKFSVKLENWRFSYISKGGRLTLIQATLSSISTYMLSVFK 72
           L  PLL        +GP L+ K  V++  W   ++S  GRL LI + + S++ + +S F+
Sbjct: 28  LGLPLLTKKMTTSDYGP-LVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFR 87

Query: 73  VPQSICKKVDKLIRTFLWCGTSVDNNLRPLISWDVVTAAKDSGGFDIFKTKVSNNALQIK 132
           +P +  K++D +  +FLW G  + N  +  ++W  V   KD GG  I   K +N      
Sbjct: 88  LPSACIKEIDSICSSFLWSGPEL-NTKKAKVAWSDVCTPKDEGGLGIRSLKEANKG---- 147

Query: 133 WLWRYYQEDQPLWKRLVNAKYTIPQAGALPTQSRFSNSRAPWFSIVKHAHHFKQFTKWGL 192
                       W   ++   T+                  W  I+KH      F K  +
Sbjct: 148 ----------SFWS--ISGNTTL--------------GSWMWKKILKHRALASGFVKHDI 189

Query: 193 RNGNKIFFWQDSWS 207
            NG+   FW D+WS
Sbjct: 208 HNGSNTSFWFDNWS 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0040037.15.5e-6633.86LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
TYK06777.11.8e-6433.86LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0041397.12.3e-6432.82LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
XP_016902461.15.2e-6433.56PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo][more]
TYK00493.16.7e-6434.65LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P0C2F63.1e-1931.55Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5A7TES32.7e-6633.86LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3C4J18.6e-6533.86LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7TIB81.1e-6432.82LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S4E2K52.5e-6433.56LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC1079... [more]
A0A5A7T9I73.3e-6432.17LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT3G24255.18.9e-1426.29RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33116:SF39RETROTRANSPOSON, UNCLASSIFIED-LIKE PROTEINcoord: 14..220
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 14..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006324.1Spg006324.1mRNA