Spg006254 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006254
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold4: 5052293 .. 5054533 (-)
RNA-Seq ExpressionSpg006254
SyntenySpg006254
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGCCTTGCAGTTGCAATTTCAATCTCGCCTTCGTGTTTCGAAGATTTTGTCTTCGACAATTTGGAAATGGGACAGAGATTCTCCTCTGCTTCACGATCCATTGCGATCGCCGGATTTCTCACTCTCGCCGGTGCAATCTCGCTGTTTTAGCTCCAATCCCATGGATGACGATTGGGCCTTCTCAAACTCTGAGCCGAGAAGCAGTCCGCCGCATCGTCGTTCTCCTCCGGATCCTCGCGAAGCTCGTCGAGTTCCCAAATTCACCGGGGAAGCATCTGCTCCTTACTCTGAAGATAATCGGAATCGACGCTTTAATAAACATTCGGAAGGCTCTTCGTTTCGATTCGCAAATGAAGGGTCGAGTTCGCGGCAGAGGAGTGAGTCTTTGTCTCATAAGGATTTTAGCTTTCTTGAAAAATTCAAGCTGAATACTGATAATCAGAGTAGTAGCAAAGAGAAGACTGAGCAGAGCTCTCCTCCTCGGTTTTCTGAATCAATGCAGGAGAAGCAATCGCAACCACAGCGTCCGCCAGAAGCTGATGAGATATTCAAGAAAATGAAGGAGACTGGTCTGATTCCCAACGCTGTCGCCATGCTTGATGGGCTGTGCAAAGATGGGCTTATCCAAGAAGCTATGAAACTCTTTGGATTGATTCGTGAGAAGGGTACAATCCCGGAAGTTGTGATTTACACCGCTGTTGTTGAAGGGTTTTGTAAGGCTGAGAAGCTTGATGAAGCAATTAGGATCTTCAGGAAAATGCAGAATAATGGCATTTCTCCTAATGCTTTCAGTTTTGGCGTCTTGATACAGGGACTGTGCAAATGCAAAAAATTAGAGGATGCTGTAGCATTTTGCATTGAGATGTTAGAATCTGGGCATTCTCCAAATCTAATCACTTTTGTTGGCTTAATTGATGAGTTGTGCAAAGAAAGGGGAGTGGAGGAAGCTCAGAGTGTTTTAGAAACCTTGAAACAAAAGGGATTCTTGATTAATGAGAAAGCTTTAAGAGAATTTCTGAACAAAAGAGCTCCCTTTTCACCTAATATCTGGGAAGTAATCTTTGGAAAGAAAACGAAGGAATTTTTCTGAGATTGTTGTGGTAGTCATAAAAGGTTGTAAGTCTTCTTTTTTTTTTTTTCCCCTTTTTATGTAATTCCTTCTCTAATTTAAGATATTTTTCTTATAGTATATGGAGTAATTCGAGCAGATGACCGACATGTTATAATAGAAATTATCTTCAATCTTTTGATAGCGTCCAATAGCTTGTACGAATTTCTGAATACAAAAATGATAAGGTACGAAACAAGGAAAAAATTAGGAAAAGAAGGGAAAAAATGCTTGTGTGTTGCCTTAGTCTTATTAAAACTTATCTCATTGTTGTTAGTTTAATCAGAATTTATTGAAATTTCTATTTTAATTAGCGATCTAGGATGATTGTAATTTGTAAAAGTGGTTCATGTAACATATCAGGAACTTTCTGGGATTTAACTATTACTTATACTGACGTAGACAGTAGGCTTCAATCTCTTAATCTCCCCCTTCCCCCACCCTCGTTTTCTCTTTTTGTTATTTTCACTAAAAAAACAGATTGTCACCGAAACAAGTTTTTTATTTCCTCTGGTCCACCTCAAGCTTACTACCCAAGTTTATAAGTTTAAGAATTACTCAAAATAAAAGCCAGCCTTCAACATATAACCAAGGACAATAAGATAAGCACACACGTTAACAGGTTCTAAAATCACTCAGCACAAGCATACAAGAACTTAATATTAAAGTTTACATGGTTAAAATTCACAAGTTTAAAGTTGCATCTTATGGGAGAGGTTGTTCACTAGTGGATGAAGCTCGACTTGAGATTAGTTGCTCATTTCATTTTGGGAATTGATGAATGGAAGTTCGTACATAATATGATGTTCAGATGGTTTGATCATTATTATGATGGATCAAATAGGAGTTGTCATTTAAAAAGGTTGCATAATTTATAGTTTGTTAACTATGGCCAAAGATATGCAGCTCCCAAGTGTTGATGTATGATGAATCAAATAGGAGTTGTGCATAATTTATATTCTGTTTACTATGGCCAAGGATATGCTGTTCCCAAGTGTTAATGCTTATCTGTATGATTATGTAGGTGAAGATACCGATCATATCACTGAGAAGAAACCACAAGTTGAAATCATTGAAACTCGGCAGCCAGAATATAGTGATTGTGTTGAAGAACAAGAATCTGGTTGA

mRNA sequence

ATGGGAGCCTTGCAGTTGCAATTTCAATCTCGCCTTCGTGTTTCGAAGATTTTGTCTTCGACAATTTGGAAATGGGACAGAGATTCTCCTCTGCTTCACGATCCATTGCGATCGCCGGATTTCTCACTCTCGCCGGTGCAATCTCGCTGTTTTAGCTCCAATCCCATGGATGACGATTGGGCCTTCTCAAACTCTGAGCCGAGAAGCAGTCCGCCGCATCGTCGTTCTCCTCCGGATCCTCGCGAAGCTCGTCGAGTTCCCAAATTCACCGGGGAAGCATCTGCTCCTTACTCTGAAGATAATCGGAATCGACGCTTTAATAAACATTCGGAAGGCTCTTCGTTTCGATTCGCAAATGAAGGGTCGAGTTCGCGGCAGAGGAGTGAGTCTTTGTCTCATAAGGATTTTAGCTTTCTTGAAAAATTCAAGCTGAATACTGATAATCAGAGTAGTAGCAAAGAGAAGACTGAGCAGAGCTCTCCTCCTCGGTTTTCTGAATCAATGCAGGAGAAGCAATCGCAACCACAGCGTCCGCCAGAAGCTGATGAGATATTCAAGAAAATGAAGGAGACTGGTCTGATTCCCAACGCTGTCGCCATGCTTGATGGGCTGTGCAAAGATGGGCTTATCCAAGAAGCTATGAAACTCTTTGGATTGATTCGTGAGAAGGGTACAATCCCGGAAGTTGTGATTTACACCGCTGTTGTTGAAGGGTTTTGTAAGGCTGAGAAGCTTGATGAAGCAATTAGGATCTTCAGGAAAATGCAGAATAATGGCATTTCTCCTAATGCTTTCAGTTTTGGCGTCTTGATACAGGGACTGTGCAAATGCAAAAAATTAGAGGATGCTGTAGCATTTTGCATTGAGATGTTAGAATCTGGGCATTCTCCAAATCTAATCACTTTTGTTGGCTTAATTGATGAGTTGTGCAAAGAAAGGGGAGTGGAGGAAGCTCAGAGTGTTTTAGAAACCTTGAAACAAAAGGGATTCTTGATTAATGAGAAAGCTTTAAGAGAATTTCTGAACAAAAGAGCTCCCTTTTCACCTAATATCTGGGAAGATATGCTGTTCCCAAGTGTTAATGCTTATCTGTATGATTATGTAGGTGAAGATACCGATCATATCACTGAGAAGAAACCACAAGTTGAAATCATTGAAACTCGGCAGCCAGAATATAGTGATTGTGTTGAAGAACAAGAATCTGGTTGA

Coding sequence (CDS)

ATGGGAGCCTTGCAGTTGCAATTTCAATCTCGCCTTCGTGTTTCGAAGATTTTGTCTTCGACAATTTGGAAATGGGACAGAGATTCTCCTCTGCTTCACGATCCATTGCGATCGCCGGATTTCTCACTCTCGCCGGTGCAATCTCGCTGTTTTAGCTCCAATCCCATGGATGACGATTGGGCCTTCTCAAACTCTGAGCCGAGAAGCAGTCCGCCGCATCGTCGTTCTCCTCCGGATCCTCGCGAAGCTCGTCGAGTTCCCAAATTCACCGGGGAAGCATCTGCTCCTTACTCTGAAGATAATCGGAATCGACGCTTTAATAAACATTCGGAAGGCTCTTCGTTTCGATTCGCAAATGAAGGGTCGAGTTCGCGGCAGAGGAGTGAGTCTTTGTCTCATAAGGATTTTAGCTTTCTTGAAAAATTCAAGCTGAATACTGATAATCAGAGTAGTAGCAAAGAGAAGACTGAGCAGAGCTCTCCTCCTCGGTTTTCTGAATCAATGCAGGAGAAGCAATCGCAACCACAGCGTCCGCCAGAAGCTGATGAGATATTCAAGAAAATGAAGGAGACTGGTCTGATTCCCAACGCTGTCGCCATGCTTGATGGGCTGTGCAAAGATGGGCTTATCCAAGAAGCTATGAAACTCTTTGGATTGATTCGTGAGAAGGGTACAATCCCGGAAGTTGTGATTTACACCGCTGTTGTTGAAGGGTTTTGTAAGGCTGAGAAGCTTGATGAAGCAATTAGGATCTTCAGGAAAATGCAGAATAATGGCATTTCTCCTAATGCTTTCAGTTTTGGCGTCTTGATACAGGGACTGTGCAAATGCAAAAAATTAGAGGATGCTGTAGCATTTTGCATTGAGATGTTAGAATCTGGGCATTCTCCAAATCTAATCACTTTTGTTGGCTTAATTGATGAGTTGTGCAAAGAAAGGGGAGTGGAGGAAGCTCAGAGTGTTTTAGAAACCTTGAAACAAAAGGGATTCTTGATTAATGAGAAAGCTTTAAGAGAATTTCTGAACAAAAGAGCTCCCTTTTCACCTAATATCTGGGAAGATATGCTGTTCCCAAGTGTTAATGCTTATCTGTATGATTATGTAGGTGAAGATACCGATCATATCACTGAGAAGAAACCACAAGTTGAAATCATTGAAACTCGGCAGCCAGAATATAGTGATTGTGTTGAAGAACAAGAATCTGGTTGA

Protein sequence

MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDWAFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANEGSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPEADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLITFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWEDMLFPSVNAYLYDYVGEDTDHITEKKPQVEIIETRQPEYSDCVEEQESG
Homology
BLAST of Spg006254 vs. NCBI nr
Match: XP_022969144.1 (pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita maxima] >XP_022969146.1 pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita maxima])

HSP 1 Score: 589.0 bits (1517), Expect = 3.2e-164
Identity = 303/353 (85.84%), Postives = 322/353 (91.22%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGALQ+Q QSRLRVSKIL STI  W+R  PLLHDPLR+PD SLSPV+SRCFSSN MDDDW
Sbjct: 1   MGALQMQLQSRLRVSKILFSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
            FS SEPRSSPP RRSPPDPREARRVPKF GE SAPY EDNRN+RFN+H EGS  RFANE
Sbjct: 61  NFSRSEPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHWEGSESRFANE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SR+RSESLS KDFSFLEKFKLNTDN S+S EKTE SS  +FSESMQ KQSQPQ PPE
Sbjct: 121 GPMSRRRSESLSQKDFSFLEKFKLNTDNLSTSTEKTEPSSSAQFSESMQGKQSQPQCPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIF+KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVV+GFC
Sbjct: 181 ADEIFRKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAE LDEAIRIFRKMQNNGISPNAFS+GVLIQGL KCKKLEDAV+FCIEMLESGHSPNL 
Sbjct: 241 KAENLDEAIRIFRKMQNNGISPNAFSYGVLIQGLYKCKKLEDAVSFCIEMLESGHSPNLT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           TFVGLIDELCKE+GV+EA +V+ETLKQKGFLINEKALREFLNKRAPFSP+IWE
Sbjct: 301 TFVGLIDELCKEKGVDEAHTVIETLKQKGFLINEKALREFLNKRAPFSPHIWE 353

BLAST of Spg006254 vs. NCBI nr
Match: XP_023554525.1 (pentatricopeptide repeat-containing protein At4g38150-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554526.1 pentatricopeptide repeat-containing protein At4g38150-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-163
Identity = 301/353 (85.27%), Postives = 324/353 (91.78%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGALQ+Q QSRLRVSKILSSTI  W+R  PLLHDPLR+PD SLSPV+SRCFSSN MDDDW
Sbjct: 1   MGALQMQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
            FS SEPRSSPP RRSPPDPREARRVPKF GE SAPY EDNRN+RFN+HSEGSS RFANE
Sbjct: 61  NFSRSEPRSSPPQRRSPPDPREARRVPKFAGEGSAPYPEDNRNQRFNRHSEGSSSRFANE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SR+R+ESLS KDFSFLEKFKLNTDNQS+S EK E SS  +FSESMQ +QSQPQ PPE
Sbjct: 121 GPMSRRRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQFSESMQGEQSQPQCPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIF+KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVV+GFC
Sbjct: 181 ADEIFRKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAEKLDEAIRIFRKMQNNGISPNAFS+G+LIQGL K K+LEDAV+FCIEMLESGHSPNL 
Sbjct: 241 KAEKLDEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCIEMLESGHSPNLT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           TFVGLIDELCKE+G +EA +V+ETLKQKGFLINEKALREFLNKRAPFSP+IWE
Sbjct: 301 TFVGLIDELCKEKGEDEAHTVIETLKQKGFLINEKALREFLNKRAPFSPHIWE 353

BLAST of Spg006254 vs. NCBI nr
Match: XP_022136091.1 (pentatricopeptide repeat-containing protein At4g38150 [Momordica charantia] >XP_022136092.1 pentatricopeptide repeat-containing protein At4g38150 [Momordica charantia])

HSP 1 Score: 585.1 bits (1507), Expect = 4.6e-163
Identity = 302/353 (85.55%), Postives = 319/353 (90.37%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           M ALQLQ QSRLR+SKI+SS+IWK +R  PLLHD LR+ DFSL P QSRCFSSN MDDDW
Sbjct: 1   MAALQLQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
           A SNSEPRS+PPHRRSPPD RE RRVPKF+   SA Y  D+RNRR NK SEGSSFRF NE
Sbjct: 61  AISNSEPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SRQRSE LS KDFSFLEKFKLNTDNQSSSKEKTEQSS PRFSES+QEKQSQPQRPPE
Sbjct: 121 GGISRQRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV+GFC
Sbjct: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAEKLDEAIRIFRKMQ NGISPNAFSFGVLIQGL KC KLE+A+ FCIEMLESGHSPN+ 
Sbjct: 241 KAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           T VGL+DELCKERG EEA+SV+ETLKQKGFL+NEKALREFLNKRAPFSPNIWE
Sbjct: 301 TLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWE 353

BLAST of Spg006254 vs. NCBI nr
Match: KAG7011839.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584.7 bits (1506), Expect = 6.0e-163
Identity = 300/353 (84.99%), Postives = 321/353 (90.93%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGALQ+Q QSRLRVSKILSSTI  W+R  PLLHDPLR+PD SLSPV+SRCFSSN MDDDW
Sbjct: 1   MGALQMQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
            FS SEPRSSPP RRSPPDPREARRVPKF GE SAPY EDNRN+RFN+HSEGSS RFANE
Sbjct: 61  NFSKSEPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHSEGSSSRFANE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SR R+ESLS KDFSFLEKFKLNTDNQS+S EK E SS  +FSESMQ KQSQPQ PPE
Sbjct: 121 GPMSRPRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQFSESMQGKQSQPQCPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIF+KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVV+GFC
Sbjct: 181 ADEIFRKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAEK DEAIRIFRKMQNNGISPNAFS+G+LIQGL K K+LEDAV+FC EMLESGHSPNL 
Sbjct: 241 KAEKFDEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCFEMLESGHSPNLT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           TFVGLIDELCKE+GV+EA +V+ETLKQKGFLINEK LREFLNKRAPFSP+IWE
Sbjct: 301 TFVGLIDELCKEKGVDEAHTVIETLKQKGFLINEKDLREFLNKRAPFSPHIWE 353

BLAST of Spg006254 vs. NCBI nr
Match: XP_022952706.1 (pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita moschata] >XP_022952707.1 pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita moschata])

HSP 1 Score: 583.2 bits (1502), Expect = 1.7e-162
Identity = 299/353 (84.70%), Postives = 322/353 (91.22%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGALQ+Q QSRLRVSKILSSTI  W+R  PLLHDPLR+PD SLSPV+SRCFSSN MDDDW
Sbjct: 1   MGALQMQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
            FS SEPRSSPP RRSPPDPREARRVPKF GE SAPY EDNRN+RFN+HSEGSS RFAN+
Sbjct: 61  NFSRSEPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHSEGSSSRFAND 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SR R+ESLS KDFSFLEKFKLNTDNQS+S EK E SS  + SESMQ KQSQPQ PPE
Sbjct: 121 GPMSRPRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQISESMQGKQSQPQCPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIF+KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVV+GFC
Sbjct: 181 ADEIFRKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAEKLDEAIRIFRKMQNNGISPNAFS+G+LIQGL K K+LEDAV+FCIEMLESGHSPNL 
Sbjct: 241 KAEKLDEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCIEMLESGHSPNLT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           TFVGLIDELCKE+GV+EA +V+ETLKQKGFLINEK LR+FLNKRAPFSP+IWE
Sbjct: 301 TFVGLIDELCKEKGVDEAHTVIETLKQKGFLINEKDLRDFLNKRAPFSPHIWE 353

BLAST of Spg006254 vs. ExPASy Swiss-Prot
Match: Q9SZL5 (Pentatricopeptide repeat-containing protein At4g38150 OS=Arabidopsis thaliana OX=3702 GN=At4g38150 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.5e-73
Identity = 158/281 (56.23%), Postives = 191/281 (67.97%), Query Frame = 0

Query: 74  RRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANEGSSSRQRSESLSH 133
           +++PP+P   R      GE S+           N H E  + +  N G S    S     
Sbjct: 42  QQNPPEPLPNR---PLRGERSS-----------NSHREPPARQAHNLGKSDTTLS----- 101

Query: 134 KDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE-ADEIFKKMKETG 193
            D  FLE+FKL  +  S    K EQ               +P  PPE +DEIFKKMKE G
Sbjct: 102 -DDGFLEQFKLGVNQDSRETPKPEQ------------YPQEPLPPPEDSDEIFKKMKEGG 161

Query: 194 LIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFCKAEKLDEAIRIF 253
           LIPNAVAMLDGLCKDGL+QEAMKLFGL+R+KGTIPEVVIYTAVVE FCKA K+++A RIF
Sbjct: 162 LIPNAVAMLDGLCKDGLVQEAMKLFGLMRDKGTIPEVVIYTAVVEAFCKAHKIEDAKRIF 221

Query: 254 RKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLITFVGLIDELCKE 313
           RKMQNNGI+PNAFS+GVL+QGL  C  L+DAVAFC EMLESGHSPN+ TFV L+D LC+ 
Sbjct: 222 RKMQNNGIAPNAFSYGVLVQGLYNCNMLDDAVAFCSEMLESGHSPNVPTFVELVDALCRV 281

Query: 314 RGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           +GVE+AQS ++TL QKGF +N KA++EF++KRAPF    WE
Sbjct: 282 KGVEQAQSAIDTLNQKGFAVNVKAVKEFMDKRAPFPSLAWE 290

BLAST of Spg006254 vs. ExPASy Swiss-Prot
Match: Q0WVK7 (Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g05670 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.8e-24
Identity = 56/157 (35.67%), Postives = 92/157 (58.60%), Query Frame = 0

Query: 180 EADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV 239
           EA+E F +M   G++P+ V    ++DG CK G I+ A K F  +  +   P+V+ YTA++
Sbjct: 334 EAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAII 393

Query: 240 EGFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHS 299
            GFC+   + EA ++F +M   G+ P++ +F  LI G CK   ++DA      M+++G S
Sbjct: 394 SGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCS 453

Query: 300 PNLITFVGLIDELCKERGVEEAQSVLETLKQKGFLIN 334
           PN++T+  LID LCKE  ++ A  +L  + + G   N
Sbjct: 454 PNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPN 490

BLAST of Spg006254 vs. ExPASy Swiss-Prot
Match: Q9FNL2 (Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX=3702 GN=At5g46100 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.3e-23
Identity = 55/150 (36.67%), Postives = 90/150 (60.00%), Query Frame = 0

Query: 183 EIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGF 242
           +IF +M + G  P++     ++ GLC+ G I EA KLF  + EK   P VV YT+++ G 
Sbjct: 178 KIFLEMPKRGCDPDSYTYGTLISGLCRFGRIDEAKKLFTEMVEKDCAPTVVTYTSLINGL 237

Query: 243 CKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNL 302
           C ++ +DEA+R   +M++ GI PN F++  L+ GLCK  +   A+     M+  G  PN+
Sbjct: 238 CGSKNVDEAMRYLEEMKSKGIEPNVFTYSSLMDGLCKDGRSLQAMELFEMMMARGCRPNM 297

Query: 303 ITFVGLIDELCKERGVEEAQSVLETLKQKG 330
           +T+  LI  LCKE+ ++EA  +L+ +  +G
Sbjct: 298 VTYTTLITGLCKEQKIQEAVELLDRMNLQG 327

BLAST of Spg006254 vs. ExPASy Swiss-Prot
Match: Q6NQ83 (Pentatricopeptide repeat-containing protein At3g22470, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g22470 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.3e-22
Identity = 46/151 (30.46%), Postives = 92/151 (60.93%), Query Frame = 0

Query: 180 EADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV 239
           EA E++ +M   G+ P+ +   +++DG CK+  + EA ++F L+  KG  P++V Y+ ++
Sbjct: 333 EAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILI 392

Query: 240 EGFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHS 299
             +CKA+++D+ +R+FR++ + G+ PN  ++  L+ G C+  KL  A     EM+  G  
Sbjct: 393 NSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVP 452

Query: 300 PNLITFVGLIDELCKERGVEEAQSVLETLKQ 328
           P+++T+  L+D LC    + +A  + E +++
Sbjct: 453 PSVVTYGILLDGLCDNGELNKALEIFEKMQK 483

BLAST of Spg006254 vs. ExPASy Swiss-Prot
Match: Q9LPX2 (Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g12775 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.3e-22
Identity = 57/152 (37.50%), Postives = 86/152 (56.58%), Query Frame = 0

Query: 181 ADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVE 240
           A E+ +KM+E  +  +AV    ++DGLCKDG +  A  LF  +  KG   +++ Y  ++ 
Sbjct: 247 AMELLRKMEERNIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIKGFKADIITYNTLIG 306

Query: 241 GFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSP 300
           GFC A + D+  ++ R M    ISPN  +F VLI    K  KL +A     EM++ G +P
Sbjct: 307 GFCNAGRWDDGAKLLRDMIKRKISPNVVTFSVLIDSFVKEGKLREADQLLKEMMQRGIAP 366

Query: 301 NLITFVGLIDELCKERGVEEAQSVLETLKQKG 330
           N IT+  LID  CKE  +EEA  +++ +  KG
Sbjct: 367 NTITYNSLIDGFCKENRLEEAIQMVDLMISKG 398

BLAST of Spg006254 vs. ExPASy TrEMBL
Match: A0A6J1I1Q2 (pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita maxima OX=3661 GN=LOC111468227 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.5e-164
Identity = 303/353 (85.84%), Postives = 322/353 (91.22%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGALQ+Q QSRLRVSKIL STI  W+R  PLLHDPLR+PD SLSPV+SRCFSSN MDDDW
Sbjct: 1   MGALQMQLQSRLRVSKILFSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
            FS SEPRSSPP RRSPPDPREARRVPKF GE SAPY EDNRN+RFN+H EGS  RFANE
Sbjct: 61  NFSRSEPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHWEGSESRFANE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SR+RSESLS KDFSFLEKFKLNTDN S+S EKTE SS  +FSESMQ KQSQPQ PPE
Sbjct: 121 GPMSRRRSESLSQKDFSFLEKFKLNTDNLSTSTEKTEPSSSAQFSESMQGKQSQPQCPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIF+KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVV+GFC
Sbjct: 181 ADEIFRKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAE LDEAIRIFRKMQNNGISPNAFS+GVLIQGL KCKKLEDAV+FCIEMLESGHSPNL 
Sbjct: 241 KAENLDEAIRIFRKMQNNGISPNAFSYGVLIQGLYKCKKLEDAVSFCIEMLESGHSPNLT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           TFVGLIDELCKE+GV+EA +V+ETLKQKGFLINEKALREFLNKRAPFSP+IWE
Sbjct: 301 TFVGLIDELCKEKGVDEAHTVIETLKQKGFLINEKALREFLNKRAPFSPHIWE 353

BLAST of Spg006254 vs. ExPASy TrEMBL
Match: A0A6J1C2J3 (pentatricopeptide repeat-containing protein At4g38150 OS=Momordica charantia OX=3673 GN=LOC111007869 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.2e-163
Identity = 302/353 (85.55%), Postives = 319/353 (90.37%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           M ALQLQ QSRLR+SKI+SS+IWK +R  PLLHD LR+ DFSL P QSRCFSSN MDDDW
Sbjct: 1   MAALQLQLQSRLRMSKIVSSSIWKCERQLPLLHDSLRTQDFSLLPTQSRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
           A SNSEPRS+PPHRRSPPD RE RRVPKF+   SA Y  D+RNRR NK SEGSSFRF NE
Sbjct: 61  AISNSEPRSNPPHRRSPPDSREPRRVPKFSDGVSASYPGDDRNRRINKRSEGSSFRFDNE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SRQRSE LS KDFSFLEKFKLNTDNQSSSKEKTEQSS PRFSES+QEKQSQPQRPPE
Sbjct: 121 GGISRQRSEFLSQKDFSFLEKFKLNTDNQSSSKEKTEQSSAPRFSESVQEKQSQPQRPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV+GFC
Sbjct: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAEKLDEAIRIFRKMQ NGISPNAFSFGVLIQGL KC KLE+A+ FCIEMLESGHSPN+ 
Sbjct: 241 KAEKLDEAIRIFRKMQKNGISPNAFSFGVLIQGLYKCNKLEEAIGFCIEMLESGHSPNVT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           T VGL+DELCKERG EEA+SV+ETLKQKGFL+NEKALREFLNKRAPFSPNIWE
Sbjct: 301 TLVGLVDELCKERGTEEARSVIETLKQKGFLLNEKALREFLNKRAPFSPNIWE 353

BLAST of Spg006254 vs. ExPASy TrEMBL
Match: A0A6J1GKZ0 (pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita moschata OX=3662 GN=LOC111455321 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 8.4e-163
Identity = 299/353 (84.70%), Postives = 322/353 (91.22%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGALQ+Q QSRLRVSKILSSTI  W+R  PLLHDPLR+PD SLSPV+SRCFSSN MDDDW
Sbjct: 1   MGALQMQLQSRLRVSKILSSTIRNWERGFPLLHDPLRTPDLSLSPVESRCFSSNSMDDDW 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
            FS SEPRSSPP RRSPPDPREARRVPKF GE SAPY EDNRN+RFN+HSEGSS RFAN+
Sbjct: 61  NFSRSEPRSSPPQRRSPPDPREARRVPKFAGEVSAPYPEDNRNQRFNRHSEGSSSRFAND 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE 180
           G  SR R+ESLS KDFSFLEKFKLNTDNQS+S EK E SS  + SESMQ KQSQPQ PPE
Sbjct: 121 GPMSRPRNESLSQKDFSFLEKFKLNTDNQSTSTEKIEPSSSAQISESMQGKQSQPQCPPE 180

Query: 181 ADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFC 240
           ADEIF+KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVV+YTAVV+GFC
Sbjct: 181 ADEIFRKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVVYTAVVDGFC 240

Query: 241 KAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLI 300
           KAEKLDEAIRIFRKMQNNGISPNAFS+G+LIQGL K K+LEDAV+FCIEMLESGHSPNL 
Sbjct: 241 KAEKLDEAIRIFRKMQNNGISPNAFSYGILIQGLYKSKQLEDAVSFCIEMLESGHSPNLT 300

Query: 301 TFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           TFVGLIDELCKE+GV+EA +V+ETLKQKGFLINEK LR+FLNKRAPFSP+IWE
Sbjct: 301 TFVGLIDELCKEKGVDEAHTVIETLKQKGFLINEKDLRDFLNKRAPFSPHIWE 353

BLAST of Spg006254 vs. ExPASy TrEMBL
Match: A0A0A0K6C0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G029950 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.5e-143
Identity = 285/355 (80.28%), Postives = 309/355 (87.04%), Query Frame = 0

Query: 1   MGALQLQFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDW 60
           MGA QL  QSRL+VSKILSSTI KW+ D PLLH+PLR+PDFSL P QSR F+SN MDDD 
Sbjct: 1   MGAFQL--QSRLQVSKILSSTIRKWEIDFPLLHNPLRTPDFSLYPAQSRRFASNSMDDDH 60

Query: 61  AFSNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANE 120
           AFS+SEP ++PP RR PPD REARRVP+  G  +A Y  DNRN+RFN+HSEGSS RF NE
Sbjct: 61  AFSDSEPINTPPQRRFPPDHREARRVPR--GGVTASY--DNRNQRFNRHSEGSSSRFTNE 120

Query: 121 GSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQ-SSPPRFSESMQEK-QSQPQRP 180
           GS+S QRSESLS KDFSFLEKFKLNTDNQSS KEKTE+ SS    SESM EK QS+PQRP
Sbjct: 121 GSTSPQRSESLSQKDFSFLEKFKLNTDNQSSGKEKTEENSSSAPVSESMLEKQQSEPQRP 180

Query: 181 PEADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEG 240
           PEADEIF KMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV+G
Sbjct: 181 PEADEIFGKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVDG 240

Query: 241 FCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPN 300
           FCKAEK DEAIRIFRKMQ+NGI PNAFSFGVLIQGL KCKKL+DAVAFC EMLESGH PN
Sbjct: 241 FCKAEKFDEAIRIFRKMQHNGIPPNAFSFGVLIQGLYKCKKLDDAVAFCNEMLESGHLPN 300

Query: 301 LITFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           L TFVGLID LC E+GV+EA SV+ET KQKGFLI+EKALRE LNKRAPFSP+IW+
Sbjct: 301 LTTFVGLIDALCNEKGVDEAHSVVETFKQKGFLIDEKALREVLNKRAPFSPDIWK 349

BLAST of Spg006254 vs. ExPASy TrEMBL
Match: A0A1S3CFW0 (pentatricopeptide repeat-containing protein At4g38150 OS=Cucumis melo OX=3656 GN=LOC103500481 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 3.3e-143
Identity = 280/349 (80.23%), Postives = 303/349 (86.82%), Query Frame = 0

Query: 7   QFQSRLRVSKILSSTIWKWDRDSPLLHDPLRSPDFSLSPVQSRCFSSNPMDDDWAFSNSE 66
           QFQSRL+VSKILSS+I KW+ D PLLH+PLR+PDFSLSP  SR FSSN MDDD AFS SE
Sbjct: 5   QFQSRLQVSKILSSSIRKWEIDFPLLHNPLRTPDFSLSPAPSRRFSSNSMDDDRAFSESE 64

Query: 67  PRSSPPHRRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANEGSSSRQ 126
           PR++PP RR PPD REARRVP+         S DNRN+RFN+HSEGSS RF NEGS+SRQ
Sbjct: 65  PRNTPPQRRFPPDHREARRVPR----GGVTDSYDNRNQRFNRHSEGSSSRFTNEGSTSRQ 124

Query: 127 RSESLSHKDFSFLEKFKLNTDNQSSSKEKTEQ-SSPPRFSESMQEK-QSQPQRPPEADEI 186
           RSESLS KDFSFLEKFKLNTDNQSS  EKTE+ SS    SESMQEK QSQ QRPP ADEI
Sbjct: 125 RSESLSQKDFSFLEKFKLNTDNQSSGNEKTEENSSSAPVSESMQEKQQSQHQRPPAADEI 184

Query: 187 FKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFCKAEK 246
           F+KMKE+GLIPNAVAMLDGLCKDGLIQEAMKLF LIREKGTIPEVVIYTAVV+GFCKAEK
Sbjct: 185 FRKMKESGLIPNAVAMLDGLCKDGLIQEAMKLFALIREKGTIPEVVIYTAVVDGFCKAEK 244

Query: 247 LDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLITFVG 306
            DEAIRIFRKMQN GISPNAFSFGVLIQGL KCKKL+DAVAFC EMLESGH PNL TFVG
Sbjct: 245 FDEAIRIFRKMQNKGISPNAFSFGVLIQGLYKCKKLDDAVAFCNEMLESGHLPNLTTFVG 304

Query: 307 LIDELCKERGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           LID LC E+GV+EA +V+ET KQKGFLI+EKALREFLNKRAPFSP+IW+
Sbjct: 305 LIDVLCSEKGVDEAHNVVETFKQKGFLIDEKALREFLNKRAPFSPDIWK 349

BLAST of Spg006254 vs. TAIR 10
Match: AT4G38150.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.5e-74
Identity = 158/281 (56.23%), Postives = 191/281 (67.97%), Query Frame = 0

Query: 74  RRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANEGSSSRQRSESLSH 133
           +++PP+P   R      GE S+           N H E  + +  N G S    S     
Sbjct: 42  QQNPPEPLPNR---PLRGERSS-----------NSHREPPARQAHNLGKSDTTLS----- 101

Query: 134 KDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE-ADEIFKKMKETG 193
            D  FLE+FKL  +  S    K EQ               +P  PPE +DEIFKKMKE G
Sbjct: 102 -DDGFLEQFKLGVNQDSRETPKPEQ------------YPQEPLPPPEDSDEIFKKMKEGG 161

Query: 194 LIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFCKAEKLDEAIRIF 253
           LIPNAVAMLDGLCKDGL+QEAMKLFGL+R+KGTIPEVVIYTAVVE FCKA K+++A RIF
Sbjct: 162 LIPNAVAMLDGLCKDGLVQEAMKLFGLMRDKGTIPEVVIYTAVVEAFCKAHKIEDAKRIF 221

Query: 254 RKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLITFVGLIDELCKE 313
           RKMQNNGI+PNAFS+GVL+QGL  C  L+DAVAFC EMLESGHSPN+ TFV L+D LC+ 
Sbjct: 222 RKMQNNGIAPNAFSYGVLVQGLYNCNMLDDAVAFCSEMLESGHSPNVPTFVELVDALCRV 281

Query: 314 RGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           +GVE+AQS ++TL QKGF +N KA++EF++KRAPF    WE
Sbjct: 282 KGVEQAQSAIDTLNQKGFAVNVKAVKEFMDKRAPFPSLAWE 290

BLAST of Spg006254 vs. TAIR 10
Match: AT4G38150.2 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.5e-74
Identity = 158/281 (56.23%), Postives = 191/281 (67.97%), Query Frame = 0

Query: 74  RRSPPDPREARRVPKFTGEASAPYSEDNRNRRFNKHSEGSSFRFANEGSSSRQRSESLSH 133
           +++PP+P   R      GE S+           N H E  + +  N G S    S     
Sbjct: 42  QQNPPEPLPNR---PLRGERSS-----------NSHREPPARQAHNLGKSDTTLS----- 101

Query: 134 KDFSFLEKFKLNTDNQSSSKEKTEQSSPPRFSESMQEKQSQPQRPPE-ADEIFKKMKETG 193
            D  FLE+FKL  +  S    K EQ               +P  PPE +DEIFKKMKE G
Sbjct: 102 -DDGFLEQFKLGVNQDSRETPKPEQ------------YPQEPLPPPEDSDEIFKKMKEGG 161

Query: 194 LIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVVEGFCKAEKLDEAIRIF 253
           LIPNAVAMLDGLCKDGL+QEAMKLFGL+R+KGTIPEVVIYTAVVE FCKA K+++A RIF
Sbjct: 162 LIPNAVAMLDGLCKDGLVQEAMKLFGLMRDKGTIPEVVIYTAVVEAFCKAHKIEDAKRIF 221

Query: 254 RKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHSPNLITFVGLIDELCKE 313
           RKMQNNGI+PNAFS+GVL+QGL  C  L+DAVAFC EMLESGHSPN+ TFV L+D LC+ 
Sbjct: 222 RKMQNNGIAPNAFSYGVLVQGLYNCNMLDDAVAFCSEMLESGHSPNVPTFVELVDALCRV 281

Query: 314 RGVEEAQSVLETLKQKGFLINEKALREFLNKRAPFSPNIWE 354
           +GVE+AQS ++TL QKGF +N KA++EF++KRAPF    WE
Sbjct: 282 KGVEQAQSAIDTLNQKGFAVNVKAVKEFMDKRAPFPSLAWE 290

BLAST of Spg006254 vs. TAIR 10
Match: AT5G03560.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 1.3e-27
Identity = 95/295 (32.20%), Postives = 150/295 (50.85%), Query Frame = 0

Query: 63  SNSEPRSSPPHRRSPPDPREARRVPKFTGEASAPYSE----DNRNRR---FNKHSEGSSF 122
           S+S   SSP    +    +E +   +F+    A +      + R RR    +K +  S F
Sbjct: 53  SSSSWWSSPDDLTASSKRKEKKTTERFSAVIDAVHDRKLPPELRGRRDFVRSKRNSNSLF 112

Query: 123 RFANEGSSSRQRSESLSHKDFSFLEKFKLNTDNQSSSKE--------KTEQSSPPRFSES 182
            +   G  +RQ S     K  +    F L+ D+     E        K E   PP    S
Sbjct: 113 PWFCGGVFARQFSSET--KRVNTKVNFSLSDDDSDEETETQVTEDSKKQEPPPPPYDPFS 172

Query: 183 MQEKQSQPQRPPEADEIFKKMKETGLIPNAVAMLDGLCKDGLIQEAMKLFGLIREKGTIP 242
            +    +P+ P    EIF KM+  G    AV M D L KDG   EA++LF  I++K  +P
Sbjct: 173 KKPAIEEPEDPKNLQEIFHKMRTEGFTNEAVKMFDALSKDGRTHEALELFSQIKDKNRMP 232

Query: 243 EVVIYTAVVEGFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKL-EDAVAF 302
           +VV +TA+VE +  A +  E +++F +M  +G+SPNA+++ VLI+GL    K  +DA  +
Sbjct: 233 DVVAHTAIVEAYANAGQAKETLKVFMRMLASGVSPNAYTYSVLIKGLAADGKTHKDAKKY 292

Query: 303 CIEMLESGHSPNLITFVGLIDELCKERGVEEAQSVLETLKQKGFLINEKALREFL 342
            +EM+ +G SPN  T+  + +   +E   E A+ +L+ +K KGF+ +EKA+RE L
Sbjct: 293 LLEMMGNGMSPNAATYTAVFEAFVREGKEESARELLQEMKGKGFVPDEKAVREAL 345

BLAST of Spg006254 vs. TAIR 10
Match: AT1G05670.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 112.8 bits (281), Expect = 6.2e-25
Identity = 56/157 (35.67%), Postives = 92/157 (58.60%), Query Frame = 0

Query: 180 EADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV 239
           EA+E F +M   G++P+ V    ++DG CK G I+ A K F  +  +   P+V+ YTA++
Sbjct: 334 EAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAII 393

Query: 240 EGFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHS 299
            GFC+   + EA ++F +M   G+ P++ +F  LI G CK   ++DA      M+++G S
Sbjct: 394 SGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCS 453

Query: 300 PNLITFVGLIDELCKERGVEEAQSVLETLKQKGFLIN 334
           PN++T+  LID LCKE  ++ A  +L  + + G   N
Sbjct: 454 PNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPN 490

BLAST of Spg006254 vs. TAIR 10
Match: AT1G05670.2 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 112.8 bits (281), Expect = 6.2e-25
Identity = 56/157 (35.67%), Postives = 92/157 (58.60%), Query Frame = 0

Query: 180 EADEIFKKMKETGLIPNAV---AMLDGLCKDGLIQEAMKLFGLIREKGTIPEVVIYTAVV 239
           EA+E F +M   G++P+ V    ++DG CK G I+ A K F  +  +   P+V+ YTA++
Sbjct: 334 EAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAII 393

Query: 240 EGFCKAEKLDEAIRIFRKMQNNGISPNAFSFGVLIQGLCKCKKLEDAVAFCIEMLESGHS 299
            GFC+   + EA ++F +M   G+ P++ +F  LI G CK   ++DA      M+++G S
Sbjct: 394 SGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCS 453

Query: 300 PNLITFVGLIDELCKERGVEEAQSVLETLKQKGFLIN 334
           PN++T+  LID LCKE  ++ A  +L  + + G   N
Sbjct: 454 PNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPN 490

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022969144.13.2e-16485.84pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita maxima] >X... [more]
XP_023554525.11.2e-16385.27pentatricopeptide repeat-containing protein At4g38150-like isoform X1 [Cucurbita... [more]
XP_022136091.14.6e-16385.55pentatricopeptide repeat-containing protein At4g38150 [Momordica charantia] >XP_... [more]
KAG7011839.16.0e-16384.99Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_022952706.11.7e-16284.70pentatricopeptide repeat-containing protein At4g38150-like [Cucurbita moschata] ... [more]
Match NameE-valueIdentityDescription
Q9SZL53.5e-7356.23Pentatricopeptide repeat-containing protein At4g38150 OS=Arabidopsis thaliana OX... [more]
Q0WVK78.8e-2435.67Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidop... [more]
Q9FNL23.3e-2336.67Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana OX... [more]
Q6NQ831.3e-2230.46Pentatricopeptide repeat-containing protein At3g22470, mitochondrial OS=Arabidop... [more]
Q9LPX21.3e-2237.50Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1I1Q21.5e-16485.84pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita maxima O... [more]
A0A6J1C2J32.2e-16385.55pentatricopeptide repeat-containing protein At4g38150 OS=Momordica charantia OX=... [more]
A0A6J1GKZ08.4e-16384.70pentatricopeptide repeat-containing protein At4g38150-like OS=Cucurbita moschata... [more]
A0A0A0K6C01.5e-14380.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G029950 PE=4 SV=1[more]
A0A1S3CFW03.3e-14380.23pentatricopeptide repeat-containing protein At4g38150 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT4G38150.12.5e-7456.23Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G38150.22.5e-7456.23Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G03560.21.3e-2732.20Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G05670.16.2e-2535.67Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G05670.26.2e-2535.67Pentatricopeptide repeat (PPR-like) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 287..338
e-value: 2.4E-5
score: 25.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 163..286
e-value: 2.3E-29
score: 104.8
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 199..224
e-value: 6.6E-5
score: 22.9
coord: 301..330
e-value: 0.14
score: 12.5
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 227..276
e-value: 1.7E-16
score: 60.1
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 199..227
e-value: 8.9E-5
score: 20.4
coord: 230..264
e-value: 7.1E-10
score: 36.4
coord: 266..298
e-value: 3.2E-6
score: 25.0
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 228..262
score: 12.945353
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 193..227
score: 8.582755
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 298..332
score: 9.010246
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 263..297
score: 11.257313
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..141
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..175
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..88
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..126
NoneNo IPR availablePANTHERPTHR45613:SF39PPR CONTAINING PLANT-LIKE PROTEINcoord: 61..355
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 61..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006254.1Spg006254.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding