Spg006232 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg006232 vs. NCBI nr
Match: XP_031744817.1 (exopolygalacturonase-like [Cucumis sativus] >KAE8645715.1 hypothetical protein Csa_020330 [Cucumis sativus])

HSP 1 Score: 671.0 bits (1730), Expect = 8.6e-189
Identity = 324/395 (82.03%), Postives = 359/395 (90.89%), Query Frame = 0

Query: 156 VALLPLLLLLVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVPKGVY 215
           +ALLP  LLL ST + QS+FDVT+YGAKPNTDITQALANAWKEAC STTPSK+L+PKGVY
Sbjct: 3   LALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVY 62

Query: 216 QLSQSNLKGPCKS-PIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGVFDGQGKAGW 275
           QLSQS LKGPCKS PI+ QV+GTL+AP HP GDGLVL  YIDQL LSGTGVFDGQGKAGW
Sbjct: 63  QLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAGW 122

Query: 276 EKNDCHQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTFQHVTITAPE 335
           +KNDCH+KKICTKLPM      +    + DITSLDSKNFHINLLGCKN+TFQHVTI+APE
Sbjct: 123 DKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPE 182

Query: 336 DSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKY 395
           +SPNTDGIH+SSSEQINILD+KI+TGDDCVSVGDSNKQ+TITNVTCGPGHGIS+GSLGKY
Sbjct: 183 NSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKY 242

Query: 396 TKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEY 455
           TKEKDVVGV VK CK+++TTNGVRIKTWPDSAGAF A+DMHFEDIEM NVSNPVIIDQEY
Sbjct: 243 TKEKDVVGVSVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEY 302

Query: 456 CPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTYSGTQ 515
           CPWNQCNRKV SKIKISKVSFKNIRGTSATP+AVKLVCSKS+PCEGVEVADI+LTYSG +
Sbjct: 303 CPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIR 362

Query: 516 GPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           GPI S+C+NV+P ITGKQ+P ICA+PAP  +PSTD
Sbjct: 363 GPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397

BLAST of Spg006232 vs. NCBI nr
Match: XP_031744814.1 (exopolygalacturonase-like [Cucumis sativus] >XP_031744815.1 LOW QUALITY PROTEIN: exopolygalacturonase [Cucumis sativus] >XP_031744816.1 exopolygalacturonase-like [Cucumis sativus] >XP_031744818.1 exopolygalacturonase-like [Cucumis sativus] >KAE8645716.1 hypothetical protein Csa_020252 [Cucumis sativus])

HSP 1 Score: 671.0 bits (1730), Expect = 8.6e-189
Identity = 324/395 (82.03%), Postives = 359/395 (90.89%), Query Frame = 0

Query: 156 VALLPLLLLLVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVPKGVY 215
           +ALLP  LLL ST + QS+FDVT+YGAKPNTDITQALANAWKEAC STTPSK+L+PKGVY
Sbjct: 3   LALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVY 62

Query: 216 QLSQSNLKGPCKS-PIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGVFDGQGKAGW 275
           QLSQS LKGPCKS PI+ QV+GTL+AP HP GDGLVL  YIDQL LSGTGVFDGQGKAGW
Sbjct: 63  QLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAGW 122

Query: 276 EKNDCHQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTFQHVTITAPE 335
           +KNDCH+KKICTKLPM      +    + DITSLDSKNFHINLLGCKN+TFQHVTI+APE
Sbjct: 123 DKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPE 182

Query: 336 DSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKY 395
           +SPNTDGIH+SSSEQINILD+KI+TGDDCVSVGDSNKQ+TITNVTCGPGHGIS+GSLGKY
Sbjct: 183 NSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKY 242

Query: 396 TKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEY 455
           TKEKDVVGV VK CK+++TTNGVRIKTWPDSAGAF A+DMHFEDIEM NVSNPVIIDQEY
Sbjct: 243 TKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEY 302

Query: 456 CPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTYSGTQ 515
           CPWNQCNRKV SKIKISKVSFKNIRGTSATP+AVKLVCSKS+PCEGVEVADI+LTYSG +
Sbjct: 303 CPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIR 362

Query: 516 GPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           GPI S+C+NV+P ITGKQ+P ICA+PAP  +PSTD
Sbjct: 363 GPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397

BLAST of Spg006232 vs. NCBI nr
Match: XP_011652893.2 (exopolygalacturonase [Cucumis sativus] >KGN64588.2 hypothetical protein Csa_014082 [Cucumis sativus])

HSP 1 Score: 670.2 bits (1728), Expect = 1.5e-188
Identity = 330/406 (81.28%), Postives = 355/406 (87.44%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQS-----VFDVTKYGAKPNTDITQALANAWKEACAST 208
           MG R +FV LLPL LLLVS AKGQS     VFDVT +GAKPN DIT  L  AWK+ACAST
Sbjct: 1   MGLRSNFVTLLPLFLLLVSNAKGQSAAGGGVFDVTSFGAKPNGDITGPLLVAWKDACAST 60

Query: 209 TPSKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGT 268
           TPSKVL+PKG Y LSQSNLKGPCKSPIE Q+EGTLQA   PKGDGL+LLEYIDQLT+SGT
Sbjct: 61  TPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQASPDPKGDGLLLLEYIDQLTVSGT 120

Query: 269 GVFDGQGKAGWEKNDCHQKKICTKLPMVNALTIV------DITSLDSKNFHINLLGCKNL 328
           GV DGQGKAGWEKNDCH KKICTKLPM   L  +      DITSLDSKN+HINLLGCKNL
Sbjct: 121 GVLDGQGKAGWEKNDCHLKKICTKLPMTLKLNFITNSIVKDITSLDSKNYHINLLGCKNL 180

Query: 329 TFQHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPG 388
           T  HVTITAPE+SPNTDGIH+SSSEQINIL+T IATGDDC+SVGD+NKQI I++VTCGPG
Sbjct: 181 TIDHVTITAPENSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQIVISDVTCGPG 240

Query: 389 HGISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVN 448
           HGISIGSLGKY+KEK+VVGV VKKCK+ STTNGVRIKTWPDS G F ATDMHFEDIEMVN
Sbjct: 241 HGISIGSLGKYSKEKEVVGVTVKKCKLTSTTNGVRIKTWPDSVGTFPATDMHFEDIEMVN 300

Query: 449 VSNPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEV 508
           VSNP+IIDQEYCPWNQCNRKV SKIKISKVSFKNIRGTSAT VAVK+VCS+S PCE VEV
Sbjct: 301 VSNPIIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATAVAVKIVCSQSNPCEEVEV 360

Query: 509 ADIDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           ADIDLTYSG++GPIKSQC+NVKP ITGKQNPPIC EPAPID PSTD
Sbjct: 361 ADIDLTYSGSEGPIKSQCANVKPVITGKQNPPICGEPAPIDGPSTD 406

BLAST of Spg006232 vs. NCBI nr
Match: KAA0045593.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK28803.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 666.0 bits (1717), Expect = 2.8e-187
Identity = 326/404 (80.69%), Postives = 360/404 (89.11%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQ---SVFDVTKYGAKPNTDITQALANAWKEACASTTP 208
           MG   +FVALLP+LLLLVS +K Q   SVFD+TKYGAKPN D+T AL +AWKEACASTTP
Sbjct: 1   MGLISNFVALLPVLLLLVSNSKAQSSGSVFDITKYGAKPNADVTAALVSAWKEACASTTP 60

Query: 209 SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGV 268
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAPA PKGDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM---VNALT---IVDITSLDSKNFHINLLGCKNLTF 328
            DGQGKAGWEKNDCH KK CTKLPM   +N +T   + DITSLDSKN+HINLLGCKNLTF
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            HVTITAP +SPNTDGIH+SSSEQINIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK+VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           IDLTYSG++GPIKSQC+NVKP I+GKQNPPIC EPAP   PS+D
Sbjct: 361 IDLTYSGSEGPIKSQCANVKPVISGKQNPPICGEPAPAGGPSSD 404

BLAST of Spg006232 vs. NCBI nr
Match: KAA0045608.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK02648.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 666.0 bits (1717), Expect = 2.8e-187
Identity = 326/404 (80.69%), Postives = 360/404 (89.11%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQS---VFDVTKYGAKPNTDITQALANAWKEACASTTP 208
           MG   +FV LLP+LLLLVS +K QS   VFD+TKYGAKPN D+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVTLLPVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTP 60

Query: 209 SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGV 268
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAPA PKGDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM---VNALT---IVDITSLDSKNFHINLLGCKNLTF 328
            DGQGKAGWEKNDCH KK CTKLPM   +N +T   + DITSLDSKN+HINLLGCKNLTF
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            HVTITAP +SPNTDGIH+SSSEQINIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK+VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           IDLTYSG++GPIKSQC+NVKP I+GKQNPPIC EPAP   PS+D
Sbjct: 361 IDLTYSGSEGPIKSQCANVKPVISGKQNPPICGEPAPAGGPSSD 404

BLAST of Spg006232 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 9.4e-122
Identity = 216/389 (55.53%), Postives = 279/389 (71.72%), Query Frame = 0

Query: 161 LLLLLVSTAKGQS-VFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVPKGVYQLSQ 220
           +LL L    + Q+ VFD+TKYGA  N DI++AL NA+KEAC ST+PS +++PKG + ++Q
Sbjct: 12  VLLFLAHFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQ 71

Query: 221 SNLKGPCKSPIEFQVEGTLQAPAHP---KGDGLVLLEYIDQLTLSGTGVFDGQGKAGWEK 280
             L+GPCKSP+E Q++ TL+AP+ P   K    + +  +DQ T+SG G+ DGQ  A WE 
Sbjct: 72  VKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWE- 131

Query: 281 NDCHQKKICTKLP---MVNAL---TIVDITSLDSKNFHINLLGCKNLTFQHVTITAPEDS 340
             C Q K C KLP     N+L   TI DIT+LDSK+FH+N+  CKNLTF    ++AP +S
Sbjct: 132 --CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANS 191

Query: 341 PNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTK 400
           PNTDGIH+S S  +NI D+  +TGDDC+SVGD  +Q+ IT VTCGPGHGIS+GSLG    
Sbjct: 192 PNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPD 251

Query: 401 EKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCP 460
           EK VVGVFV+ C   +T NGVRIKTWP S       D+HFEDI + NVSNPV+IDQ YCP
Sbjct: 252 EKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGV-VNDVHFEDIIVQNVSNPVVIDQVYCP 311

Query: 461 WNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTYSGTQGP 520
           +N+CN+ + S++KISKVSF+NI+GTS T  AV L+ SK +PCEG+EV DID+TYSG +GP
Sbjct: 312 FNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGP 371

Query: 521 IKSQCSNVKPTITGKQNPPICAEPAPIDS 540
            KS C N+KP++ GKQNPP+C   A   S
Sbjct: 372 AKSSCENIKPSLKGKQNPPVCTASAASSS 396

BLAST of Spg006232 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.5e-119
Identity = 210/359 (58.50%), Postives = 262/359 (72.98%), Query Frame = 0

Query: 187 DITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHP-- 246
           D TQAL  AWKEACAS +PS +LVPKG + +    L+GPCKS I  Q++GTL+APA P  
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 247 -KGDGLVLLEYIDQLTLSGTGVFDGQGKAGWEKNDCHQK-KICTKLPM------VNALTI 306
            KG G + L  ID LT+ G GVFDGQGK+ W +NDCH+   IC  L M      V    +
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 307 VDITSLDSKNFHINLLGCKNLTFQHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDD 366
            D+T+LDSKNFH+N++GCKNLTF+   I+A E S NTDGIH+  S+ +NI++T+I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 367 CVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTW 426
           C+S+GD +K I ITN+TCGPGHGIS+GSLG+Y  E+ VVG++VK C I  + NGVRIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 427 PDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTS 486
           P S     A++MHF+DI M +V  P++IDQ YCP+NQC  +V S +K+SK+SFKNI+GTS
Sbjct: 241 PKSEPG-EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 487 ATPVAVKLVCSKSLPCEGVEVADIDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPA 536
            T  AVKLVCSKS PC GVE+ADIDLTYSG  GP  S C N+KPTI GKQ P IC+  A
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICSGSA 358

BLAST of Spg006232 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.1e-117
Identity = 208/393 (52.93%), Postives = 272/393 (69.21%), Query Frame = 0

Query: 157 ALLPLLLLLVSTAKGQS-VFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVPKGVY 216
           A++   L +      QS V D++K+G KPN+DI QAL +AW EACASTT +K+++P G Y
Sbjct: 6   AIIVSFLFIADFCAAQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTY 65

Query: 217 QLSQSNLKGPCKSPIEFQVEGTLQAPAHP---KG-DGLVLLEYIDQLTLSGTGVFDGQG- 276
           QL+   LKGPCK+PIE QV+GT+QAPA P   KG +      Y+D LTLSG GVFDGQG 
Sbjct: 66  QLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGA 125

Query: 277 --------KAGWEKNDCHQKKICT-KLPMVNALTIVDITSLDSKNFHINLLGCKNLTFQH 336
                    + W   + + K         VN   +  +TS DSKNFH+ + GCKN+TF  
Sbjct: 126 TVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDG 185

Query: 337 VTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGIS 396
            TITAP DSPNTDGIH+  S  + IL+T I TGDDCVS+GD +KQIT+  V CGPGHG+S
Sbjct: 186 FTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLS 245

Query: 397 IGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNP 456
           +GSLGK+T E++V G+ VK C + +T NGVRIKTWPD+ G  + +D+HFEDI M NV NP
Sbjct: 246 VGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNP 305

Query: 457 VIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADID 516
           VIIDQEY PWNQC++K  SKIK+SK+SFKN++GTS T   V L+CS ++PC+GVE+ ++D
Sbjct: 306 VIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVD 365

Query: 517 LTYSGTQGPIKSQCSNVKPTITGKQNPPICAEP 535
           L ++G   P  ++C+NVKP +TG    P+C  P
Sbjct: 366 LKFNG--APTTAKCTNVKPLVTG--TAPVCQAP 394

BLAST of Spg006232 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.7e-115
Identity = 206/394 (52.28%), Postives = 275/394 (69.80%), Query Frame = 0

Query: 157 ALLPLLLLLVSTAKGQS-VFD-VTKYGAKPN--TDITQALANAWKEACASTTPSKVLVPK 216
           ++  LLLL +S +K QS  FD V K+GAK +  TD+++   +AWKEACAS TPS V++PK
Sbjct: 10  SMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPK 69

Query: 217 GVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHP---KGDGLVLLEYIDQLTLSGTGVFDGQ 276
           G Y LS+ NL+GPCK+PIE  V+GT+QAPA P   K    V    ++   + G G+FDGQ
Sbjct: 70  GTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQ 129

Query: 277 GKAGWEKNDCHQKKICTKLP-------MVNALTIVDITSLDSKNFHINLLGCKNLTFQHV 336
           G   +EKN C  ++  +KLP       + NAL I DITS DSK FHIN+  CKN+T + +
Sbjct: 130 GSIAYEKNTCENREFRSKLPVNIRFDFLTNAL-IQDITSKDSKLFHINVFACKNITLERL 189

Query: 337 TITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISI 396
            I AP++SPNTDGIH+  SE +NI+ + I TGDDC+S+GD  K + I  +TCGPGHGISI
Sbjct: 190 KIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISI 249

Query: 397 GSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPV 456
           GSLGK+  E+ V G+ +  C I +T+NG RIKTWP   G  + +++HFEDI M NVS+P+
Sbjct: 250 GSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGG-AVSEIHFEDITMNNVSSPI 309

Query: 457 IIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDL 516
           +IDQ+YCPWN+C +   SK+K+S +SFKNIRGTSA P A+K +CS S PC+ VE+ADID+
Sbjct: 310 LIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI 369

Query: 517 TYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAP 537
            ++G + P  SQC NVKP  +GK NP  C+ P P
Sbjct: 370 KHNGAE-PATSQCLNVKPITSGKLNPIPCSGPVP 400

BLAST of Spg006232 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.1e-114
Identity = 205/394 (52.03%), Postives = 273/394 (69.29%), Query Frame = 0

Query: 157 ALLPLLLLLVSTAKGQ-SVFD-VTKYGAKPN--TDITQALANAWKEACASTTPSKVLVPK 216
           ++  LLLL +S +K Q   FD V K+GAK +  TD+++   +AWKEACAS TPS V++PK
Sbjct: 10  SMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPK 69

Query: 217 GVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHP---KGDGLVLLEYIDQLTLSGTGVFDGQ 276
           G Y LS+ NL+GPCK+PIE  V+GT+QAPA P   K    V    ++   + G G+FDGQ
Sbjct: 70  GTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQ 129

Query: 277 GKAGWEKNDCHQKKICTKLP-------MVNALTIVDITSLDSKNFHINLLGCKNLTFQHV 336
           G   +EKN C  ++  +KLP       + NAL I DITS DSK FHIN+  CKN+T + +
Sbjct: 130 GSIAYEKNTCENREFRSKLPVNIRFDFVTNAL-IQDITSKDSKLFHINVFACKNITLERL 189

Query: 337 TITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISI 396
            I AP++SPNTDGIH+  SE +NI+ + I TGDDC+S+GD  K + I  +TCGPGHGISI
Sbjct: 190 KIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISI 249

Query: 397 GSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPV 456
           GSLGK+  E+ V G+ +  C I +T+NG RIKTWP   G  + +++HFEDI M NVS+P+
Sbjct: 250 GSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGG-AVSEIHFEDITMNNVSSPI 309

Query: 457 IIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDL 516
           +IDQ+YCPWN+C +   SK+K+S +SFKNIRGTSA P A+K +CS S PC+ VE+ADID+
Sbjct: 310 LIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI 369

Query: 517 TYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAP 537
            ++G + P  SQC NVKP   GK NP  C+ P P
Sbjct: 370 QHNGAE-PATSQCLNVKPITIGKLNPIPCSGPVP 400

BLAST of Spg006232 vs. ExPASy TrEMBL
Match: A0A5A7TRZ0 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00600 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 1.3e-187
Identity = 326/404 (80.69%), Postives = 360/404 (89.11%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQS---VFDVTKYGAKPNTDITQALANAWKEACASTTP 208
           MG   +FV LLP+LLLLVS +K QS   VFD+TKYGAKPN D+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVTLLPVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTP 60

Query: 209 SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGV 268
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAPA PKGDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM---VNALT---IVDITSLDSKNFHINLLGCKNLTF 328
            DGQGKAGWEKNDCH KK CTKLPM   +N +T   + DITSLDSKN+HINLLGCKNLTF
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            HVTITAP +SPNTDGIH+SSSEQINIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK+VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           IDLTYSG++GPIKSQC+NVKP I+GKQNPPIC EPAP   PS+D
Sbjct: 361 IDLTYSGSEGPIKSQCANVKPVISGKQNPPICGEPAPAGGPSSD 404

BLAST of Spg006232 vs. ExPASy TrEMBL
Match: A0A5D3DYI3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00050 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 1.3e-187
Identity = 326/404 (80.69%), Postives = 360/404 (89.11%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQ---SVFDVTKYGAKPNTDITQALANAWKEACASTTP 208
           MG   +FVALLP+LLLLVS +K Q   SVFD+TKYGAKPN D+T AL +AWKEACASTTP
Sbjct: 1   MGLISNFVALLPVLLLLVSNSKAQSSGSVFDITKYGAKPNADVTAALVSAWKEACASTTP 60

Query: 209 SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGV 268
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAPA PKGDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM---VNALT---IVDITSLDSKNFHINLLGCKNLTF 328
            DGQGKAGWEKNDCH KK CTKLPM   +N +T   + DITSLDSKN+HINLLGCKNLTF
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            HVTITAP +SPNTDGIH+SSSEQINIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK+VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           IDLTYSG++GPIKSQC+NVKP I+GKQNPPIC EPAP   PS+D
Sbjct: 361 IDLTYSGSEGPIKSQCANVKPVISGKQNPPICGEPAPAGGPSSD 404

BLAST of Spg006232 vs. ExPASy TrEMBL
Match: A0A5A7TR51 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00990 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.9e-187
Identity = 326/404 (80.69%), Postives = 360/404 (89.11%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQS---VFDVTKYGAKPNTDITQALANAWKEACASTTP 208
           MG   +FVALL +LLLLVS +K QS   VFD+TKYGAKPN D+T ALA+AWKEACASTTP
Sbjct: 1   MGLISNFVALLSVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTP 60

Query: 209 SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGV 268
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAPA PKGDGL+LLEY+DQLT+SGTGV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGV 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM---VNALT---IVDITSLDSKNFHINLLGCKNLTF 328
            DGQGKAGWEKNDCH KK CTKLPM   +N +T   + DITSLDSKN+HINLLGCKNLTF
Sbjct: 121 LDGQGKAGWEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            HVTITAP +SPNTDGIH+SSSEQINIL+T IATGDDC+SVGD+NKQ+ I++VTCGPGHG
Sbjct: 181 DHVTITAPGNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           ISIGSLGKY+KEK+VVGV VKKCK+  TTNGVRIKTWPDSA AF A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NPVIIDQEYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVK+VCS+S PCEGVEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVAD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           IDLTYSG++GPIKSQC+NVKP I+GKQNPPIC EPAP   PS+D
Sbjct: 361 IDLTYSGSEGPIKSQCANVKPVISGKQNPPICGEPAPAGGPSSD 404

BLAST of Spg006232 vs. ExPASy TrEMBL
Match: A0A5D3D708 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00680 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.9e-186
Identity = 327/397 (82.37%), Postives = 355/397 (89.42%), Query Frame = 0

Query: 156 VALLPLLLL--LVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVPKG 215
           +ALLP LLL  L ST +GQS+FDVT YGAKPNTDITQALANAW EACASTTPS +L+PKG
Sbjct: 4   LALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIPKG 63

Query: 216 VYQLSQSNLKGPCKS-PIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGVFDGQGKA 275
           VYQLSQSNLKGPCKS PI+ QVEGTLQAP H KGDGLVL  YIDQL LSG+GVFDGQGK+
Sbjct: 64  VYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQGKS 123

Query: 276 GWEKNDCHQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTFQHVTITA 335
           GWEKN+CH+KKICTKLPM      V   T+ DITSLDSKNFHINLLGCKN+TFQHVTI A
Sbjct: 124 GWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTIIA 183

Query: 336 PEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLG 395
           P++SPNTDGIH+SSSE+I ILD+KIATGDDCVSVGDSNKQ+TITNVTCGPGHGISIGSLG
Sbjct: 184 PDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGSLG 243

Query: 396 KYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQ 455
           KYTKEKDVVGV VK CKI +TTNGVRIKTWPDSA AF A+DMHFEDIEM NVSNP+IIDQ
Sbjct: 244 KYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIIIDQ 303

Query: 456 EYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTYSG 515
           EYCPWNQCNRKV SKIKIS VSFKNIRGTSATPVAVKLVCSKS+PCEGVEVADI+LTYSG
Sbjct: 304 EYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTYSG 363

Query: 516 TQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
            +GPI S+C+NV PTITG QNP ICA+PA I +PSTD
Sbjct: 364 NRGPIVSECANVMPTITGVQNPQICAKPATIGAPSTD 400

BLAST of Spg006232 vs. ExPASy TrEMBL
Match: A0A5A7ULW5 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006240 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 7.3e-186
Identity = 323/404 (79.95%), Postives = 357/404 (88.37%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQS---VFDVTKYGAKPNTDITQALANAWKEACASTTP 208
           MG R +FVALL L LLLVS +K QS   VFDVT +GAKP+ DIT AL +AWKE CAS TP
Sbjct: 1   MGLRSNFVALLLLFLLLVSNSKAQSSGAVFDVTSFGAKPDADITGALVSAWKEGCASITP 60

Query: 209 SKVLVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKGDGLVLLEYIDQLTLSGTGV 268
           SKVL+PKG Y LSQSNLKGPCKS IE Q+EGTLQAP+ PKGDGL++LEY+D+LT+SG GV
Sbjct: 61  SKVLIPKGSYGLSQSNLKGPCKSSIELQIEGTLQAPSDPKGDGLLILEYVDRLTVSGMGV 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM---VNALT---IVDITSLDSKNFHINLLGCKNLTF 328
            DGQGK GWEKNDCH KKICTKLPM   +N +T   + DITSLDSKN+HINLLGCKNLTF
Sbjct: 121 LDGQGKEGWEKNDCHLKKICTKLPMNLKLNFITNSIVKDITSLDSKNYHINLLGCKNLTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            HVTITAP DSPNTDGIH+SSSE+INIL+T IATGDDC+SVGD+NKQI I++VTCGPGHG
Sbjct: 181 DHVTITAPGDSPNTDGIHVSSSEEINILNTNIATGDDCISVGDTNKQIVISDVTCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           ISIGSLGKY+KEK+VVGV VKKCK+ STTNGVRIKTWPDS G F A+DMHFEDIEMVNVS
Sbjct: 241 ISIGSLGKYSKEKEVVGVTVKKCKLTSTTNGVRIKTWPDSVGTFPASDMHFEDIEMVNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NPVIIDQEYCPWNQCNRKV SKIKISKVSFKNIRGTSATPVAVK+VCSKS PCE VEVAD
Sbjct: 301 NPVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPVAVKIVCSKSNPCEEVEVAD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSPSTD 544
           IDLTYSG++GPIKSQC+NVKP I+GKQNPPIC EPAP+D PSTD
Sbjct: 361 IDLTYSGSEGPIKSQCANVKPVISGKQNPPICGEPAPLDGPSTD 404

BLAST of Spg006232 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 193/382 (50.52%), Postives = 264/382 (69.11%), Query Frame = 0

Query: 162 LLLLVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVPKGVYQLSQSN 221
           ++ L+  +    VF +   G+   +DITQAL  A+  AC S++PSKV++PKG ++L +  
Sbjct: 12  IICLLGISANAEVFTI---GSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIE 71

Query: 222 LKGPCKSPIEFQVEGTLQAP--AHPKGDGLVLLEYIDQLTLSGTGVFDGQGKAGWEKNDC 281
           ++GPCK+PIE  ++GT++A   A    +  V+   ID   L+G G FDG+G A W  N+C
Sbjct: 72  MRGPCKAPIEVTLQGTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNC 131

Query: 282 HQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTFQHVTITAPEDSPNT 341
           H+   C KLP+      +    I DI+S+D+KNFHIN+LG KN+T  ++ I APEDSPNT
Sbjct: 132 HKTFECKKLPISIRFDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNT 191

Query: 342 DGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEKD 401
           DGIHL  S+ + IL++ I+TGDDC+SVGD  K + +  VTCGPGHGIS+GSLG+Y  E+D
Sbjct: 192 DGIHLGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQD 251

Query: 402 VVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWNQ 461
           V G+ V  C +  T NG+RIKTWP +A + +A+D+HFEDI + +VSNP++IDQEYCPWNQ
Sbjct: 252 VSGIKVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQ 311

Query: 462 CNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTYSGTQGPIKS 521
           CN++ AS IK+  +SFKNIRGTS    AVKL+CSK  PC+ VE+ DID+ Y+G  GP   
Sbjct: 312 CNKQKASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATF 371

Query: 522 QCSNVKPTITGKQNPPICAEPA 536
            CSNV P I G Q+P  C+ PA
Sbjct: 372 HCSNVSPKILGSQSPKACSAPA 390

BLAST of Spg006232 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.5e-111
Identity = 201/397 (50.63%), Postives = 269/397 (67.76%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKV 208
           MGS +   A+L ++ LL  +A  + VF+V   G  P +DITQAL  A+  AC + T SKV
Sbjct: 1   MGSYLGISAVLFVISLLGFSANAE-VFNV---GGSPGSDITQALLKAFTSACQAPTASKV 60

Query: 209 LVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQA--PAHPKGDGLVLLEYIDQLTLSGTGVF 268
           ++ KG ++L +  + GPCK+P+E  ++GTL+A   A    +  V+   I+   L+G G+F
Sbjct: 61  VITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIF 120

Query: 269 DGQGKAGWEKNDCHQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTFQ 328
           DG+G A W  N+CH+   C KLP+      V    I DI+S+D+KNFHIN+LG KN+TF 
Sbjct: 121 DGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFD 180

Query: 329 HVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGI 388
           +V + AP +SPNTDGIHL  SE + IL++KIATGDDC+SVGD  K + + NV CGPGHGI
Sbjct: 181 NVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGI 240

Query: 389 SIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSN 448
           S+GSLG+Y  E+DV G+ V  C +  T NG+RIKTWP +A A +A+ +HFE+I + NVSN
Sbjct: 241 SVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSN 300

Query: 449 PVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADI 508
           P++IDQEYCPWNQCN++  S IK+  +SFKNIRGTS    AVKL+CSK+ PC  VE+ +I
Sbjct: 301 PILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNI 360

Query: 509 DLTYSGTQGPIKSQCSNVKPTITGKQNPPIC-AEPAP 537
           +L Y G  GP    CSNV P + G QNP  C A P P
Sbjct: 361 NLEYKGADGPPTFMCSNVSPKLVGTQNPKACSAGPTP 393

BLAST of Spg006232 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 390.6 bits (1002), Expect = 2.1e-108
Identity = 191/401 (47.63%), Postives = 265/401 (66.08%), Query Frame = 0

Query: 149 MGSRIDFVALLPLLLLLVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKV 208
           MGS      +  + LL  S     +  +V +  + P +DITQAL  A+  AC S TP KV
Sbjct: 1   MGSYFGISTIFVICLLGFSA----NAHEVLRLTSSPGSDITQALLRAFTTACQSPTPRKV 60

Query: 209 LVPKGVYQLSQSNLKGPCKSPIEFQVEGTLQAPA---HPKGDGLVLLEYIDQLTLSGTGV 268
           ++PKG ++L +  + GPCKSP+E  + GT+ A     H K +  V+ + +D   L+G G 
Sbjct: 61  VIPKGQFKLGEIMMSGPCKSPVEITLLGTVLADGNSIHGK-EKWVVFQRMDGFRLNGGGT 120

Query: 269 FDGQGKAGWEKNDCHQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTF 328
           FDG+G A W  N+CH+   C KLP+      V    I DI+S+D+KNFHIN++G KN+TF
Sbjct: 121 FDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTF 180

Query: 329 QHVTITAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHG 388
            +V I AP +SPNTDGIHL  S  ++I++++I+TGDDCVSVGD    + + NV CGPGHG
Sbjct: 181 DNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHG 240

Query: 389 ISIGSLGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVS 448
           IS+GSLG+Y  E+DV G+ V  C +  T NG+RIKTWP +A + +A+++HFE+I + NVS
Sbjct: 241 ISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVS 300

Query: 449 NPVIIDQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVAD 508
           NP++IDQEYCPWNQCN++ +S IK++ +SF+ IRGTS    AVKL+CSK  PCE V+V D
Sbjct: 301 NPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGD 360

Query: 509 IDLTYSGTQGPIKSQCSNVKPTITGKQNPPICAEPAPIDSP 541
           I++ Y+G  GP    CSNV+P + G Q P  C  P  +  P
Sbjct: 361 INIQYTGADGPATFMCSNVRPKLVGTQFPKACNTPPVLTQP 396

BLAST of Spg006232 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.8e-107
Identity = 187/390 (47.95%), Postives = 258/390 (66.15%), Query Frame = 0

Query: 152 RIDFVALLPLLLLLVSTAKGQSVFDVTKYGAKPNTDITQALANAWKEACASTTPSKVLVP 211
           R+ F      +  L+  +    +F++    + P +DIT AL  A+ EAC   T S V++P
Sbjct: 3   RVHFGVSAFFVFCLLGLSANAKIFNI---NSPPGSDITNALLKAFNEACQFPTKSTVMIP 62

Query: 212 KGVYQLSQSNLKGPCKSPIEFQVEGTLQAPAHPKG-DGLVLLEYIDQLTLSGTGVFDGQG 271
           KG Y+L +  + GPCK+PI   + GT++A  +  G +  V    I+   L+G GVFDG+G
Sbjct: 63  KGEYKLGEIVMMGPCKAPIRIALLGTVKADGNANGKEKWVAFRNINGFKLNGGGVFDGEG 122

Query: 272 KAGWEKNDCHQKKICTKLPM------VNALTIVDITSLDSKNFHINLLGCKNLTFQHVTI 331
            A W  N+CH+   C KLP+      V    I  ITSLD+K+FHIN++G KN+TF+ V I
Sbjct: 123 NAAWRVNNCHKTFNCKKLPISIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKI 182

Query: 332 TAPEDSPNTDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGS 391
            AP +SPNTDGIH+  S+ I I+++ I+TGDDCVSVGD  K + +  VTCGPGHGISIGS
Sbjct: 183 IAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGS 242

Query: 392 LGKYTKEKDVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVII 451
           LG+Y+ E++V G+ +  C +  T NG+RIKTWP +A   +A+D+HFE+I + NVSNP++I
Sbjct: 243 LGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILI 302

Query: 452 DQEYCPWNQCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTY 511
           DQEYCPWNQCN++  S IK++ +SFK IRGTS    AVKL+CSK  PC+ VEV D+++ Y
Sbjct: 303 DQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQY 362

Query: 512 SGTQGPIKSQCSNVKPTITGKQNPPICAEP 535
           +G  GP   QCSNV P + G Q P  C+ P
Sbjct: 363 TGADGPATFQCSNVSPKLVGTQIPKACSSP 389

BLAST of Spg006232 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 2.0e-98
Identity = 187/383 (48.83%), Postives = 242/383 (63.19%), Query Frame = 0

Query: 166 VSTAKGQSVFDVTKYGAK--PNTDITQALANAWKEACASTTPSKVLVPKGVYQLSQSNLK 225
           VS AK  +  DV   GAK    TD + A A AWKEACA+   S + VPKG Y +     K
Sbjct: 61  VSGAKPGAAVDVKASGAKGDSKTDDSAAFAAAWKEACAA--GSTITVPKGEYMVESLEFK 120

Query: 226 GPCKSPIEFQVEGTLQAPAHPKGD----GLVLLEYIDQLTLSGT-GVFDGQGKAGWEKND 285
           GPCK P+  ++ G  +APA  K      G +  E I   TL+G   +FDGQG   W+ ND
Sbjct: 121 GPCKGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKAND 180

Query: 286 CHQKKICTKLPMVNALT------IVDITSLDSKNFHINLLGCKNLTFQHVTITAPEDSPN 345
           C +   C  LP+    T      I  ITS +SK FH+N+L CKN+T   + I AP +S N
Sbjct: 181 CAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLN 240

Query: 346 TDGIHLSSSEQINILDTKIATGDDCVSVGDSNKQITITNVTCGPGHGISIGSLGKYTKEK 405
           TDGIH+  S  +N++  KI TGDDCVS+GD  + + + NV CGPGHGISIGSLG+Y  E+
Sbjct: 241 TDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQ 300

Query: 406 DVVGVFVKKCKIMSTTNGVRIKTWPDSAGAFSATDMHFEDIEMVNVSNPVIIDQEYCPWN 465
            V GV V+KC I +T NGVRIKTWP S     A+++ FEDI M NVS PV+IDQEYCP+ 
Sbjct: 301 PVKGVTVRKCLIKNTDNGVRIKTWPGSPPGI-ASNILFEDITMDNVSLPVLIDQEYCPYG 360

Query: 466 QCNRKVASKIKISKVSFKNIRGTSATPVAVKLVCSKSLPCEGVEVADIDLTYSGTQGPIK 525
            C   V S++K+S V+ K I+GTSAT VAVKL+CSK +PC  + ++DI+L ++G +GP  
Sbjct: 361 HCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAV 420

Query: 526 SQCSNVKPTITGKQNPPICAEPA 536
           S CSN+KP ++GK  P  C E A
Sbjct: 421 SACSNIKPILSGKLVPAACTEVA 440