Spg006211 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg006211
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	kinetochore protein NUF2 homolog
Location	scaffold4: 4061554 .. 4066631 (-)
RNA-Seq Expression	Spg006211
Synteny	Spg006211

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGAAGAGACGTAATTGGCGATGTCAAAGTATGAATTTCCGAGGCTTCCACGGGCAGATATTGCATTGCTACTAGCACAGTGTCAAATTGCTGCTGTAACAGAGCGGGACCTTCTTCACCCGTCACCAGATTTGGTTTCAGATCTTTACACACGTTTAATGATCTACCTTGACTTGCTCCATGAGTAATTCCTCTGCTTATGGCATTTCCTTTTAACAAGTATTTGAACTGTTTTTTTGAAAGGAAGGAGAAAGAGAAAAGTTTATTTATGTGTTACTATGTCAGTCATCATTCACTTCTTTCAACGTTTTGATATCTGTGAGGTTTATAGCGTGGATTTGATGTCAAACAACTTTACTTGGTCAACTTTTTATTTCTTGCTTCTAAGAAAATTTAATATTGCATAGAATAGTAGGATAATGGTTTTATCTCTCCGTTTGTTGATAATAGGAATTGGTGTGACCCATAATTCACGTTTTGTTTCCTAGAGAATTCAAATTATGGAAGTTTTTTCTTCTCTTTGGAAGAGTAAAATACCAAAGAAGGTCAAAGTCTTTGTGCGGTAGGTTTTGCACGGATTTGTCTTTATAGGTGTCTATTTCCTGACCTTTTGGTAATTAGGAGTTTGGTTTGGTCCTCTTGGATTGAAGTCCGTTTCTTTAGCTAGTGTTTGAACTCTTTTTTGTCTATTTCCTGACCTTTTGGTAATTAGGAGTTTGGTTTGGTCCTCTTGGATTGAAGTCCGTTTCTTTAGCTAGTGTTTGAACTCTTTTTTGTGGGCTTATTGTTGTATGCTCTTGTACATTCTTTCATTTTTTCAATGAAAGCTCGGTTTCTTACTTAAAAAAATATCCATGTGTTCATGAATTTCCCTTACTCCTTTTCCATTCTAGATCTTTGGAGTGTCATACTGTGAACTTGTTTATTTCTCTATGCTTAGAATCCTTTTTAAGAAAGGTTATATTTCAATTTTGTTCGTTATTTGTGCAGGGAAGACCAAGGACAAGTTGAGTTTGCTGCCTTGGATCAGCTCGAGAACCCTGATCTGCATATGGATTCAGTTCGGATAATGAAGTTGCACAACAGAATAAAGCATGTCATTGCCTCTCTTGACTGTCCAAAAAAGTTTACATTGAAAGATTTGATAAAACCTGAAGGAGATAGGACCGAAATTTTCCTTAGTGCAATGTTGAACTTTTGCATTCACAAGTACGAACCTTATCTTCTTCTTCTCTATGGTCGTCACTTTCTCTTTACGAGAAAAAAAAAAAGAACTAGTGTAACAAACTATTAGTTTGACCTCGAAGGTCTTTATGGGTTTATCAGCTAACTTAGGGTGTAGTATCTCAACCAAGGAATTTCTACTCAATTCTCAGCTTATTATTGGGATTCTAGTCAATTGTAAATAATCGTAAGTAAACATCTCAATTAAATGAGTTTATTATTTAACTTACTACTTATGTTTGCCCCATTTATATTAGTTAGCTCATCACATTATATTGAATGAAAGCGCCAGCTTCTTGCTTATTGTTAATTTGATCATAGGCTTAATTAGATGTTCTTGTAGTTCAATGGCATTGTGCACAAGTCAACATATTCTGATAAATTTTCTAGCTTGTCACGGTAGGTTTAGTTTCTTTATGATTGGATGAATGAGATGAAGTAACAAAAACTTATCACTTCAATTTATTATATTTTATTGTTGTCATGCTTTCTTATTTGTAATACATCCCACGGGCTGGGACACAGCTCCATGCTTTCGTGTTGGTAATTACATCCAAAAGCTCAATTTTCTTATTGAATAGGCAGGGACTTCCCTACAGGGTGCTTTGATAAGGATATGTATTATTTTATTGTCTGTAGATGTTGCATGATATATATCTATCGAAATATGGTTTTTCATAAAAGAATAGATAGATTTATATCGACTCAGTTATGACTCTTGTATTTCCATTTGAAGAGATGCCAAAATCACCCTCCATACTCCAGTTATGGATGAGCTGAGTACTCTTGGTGACCAGCAAAGGGAATGGGAGGTTAAGATTTCTCAGGTATTTAGATCTCATCCCTTTTTCATTTTTATTTAATATTATTTTATTTTAAATTCGAAAAAGGGTGGACATATATATTTCTTTTTCCATTTGGCTGAACTTGTGTGTTACAGTTGAATGCAGAGATCGCAGAGTACAATGAAGCAAGAGAAAGGGAAATACCTTTTGTTCAAGAGATTGATGCTAAAGTGAAAGAACTACAACAGACTATTGGAGGACTTAACAATCAGCAGATGTCATTGCGTGCTTCTATCCGAAAGTTGAAAGACAAAGCTGGAGAAATGGATGAGAAGGTTAGATTATTAACAACTTTTTCTTTTTTCTATTTCTCTTTTTGGGATAGGGAACAGATACTTGTATATTCCTTGTTGAGAGAAGTTCCTCAAGTCGAACCATTTCTTCACAAACATTAACATAGCAGTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAAATGGTTGATGATAAGGTGTGATCAGATTGACACTAATCATAGCCATCTTGCTTTAACACATTTATAAAGTTTTCAAACCAAGCCCGAGGTGATTGTTTTAGGCTAAGAAGCACGGACACTTTAGTTTGGCTAGCGTGTCCGTGTCGGACACGTGTCGGACACTTGGACACTTGTTGGACACGTATCGGACACTTGTTAGCACAATTGATATGTGGCAAACACTAGTTGTAGGTTCATTTCAAACCCTTGTTATGTATACTAAATAGACACAATGCTTAAATGACAAAAATAATAAATTTTATAAGTAAAATACATTAAATTCATTTTTTCAAGCATACAAATGCATAAACTAATTGATTTTGAATTTCCTTCTGGGATAAAAACAATATATATTTTAAAAAATGTATATTTTTCTAAATGTGTCCTTGCCGTGTCCGTGTCCTAGATTTAAAAAAAAATAACGTGTCACCGTGTCCGTGTCGTGTCGTATCCGTGTCTCGTGTCCGTATCCGTGCTTCTTAGGTCTTAGGCTATAAAAAGGTTTCTTGAGTTTACATTCCTTTCCTTTAGTATAATGATCCTCAAATCTAAGAGATGTATCCATATGCATATCTTGTATTGGCCAACATTAAAAATGTATTTTTTACATTGAATAGAAATAGTAGTCAATCTAAATATGTTACAATAGAGAGGTACCCAAATAATATTTAACTCAGTTGTTGGAGCAAACGTTTCCTAATAGTCCATCGCTTAAGCTTGGATGAATCCTTTAACCACAATACAAAAGGGTCCAATATTAGAGTTGAAAAAATCATGAGCTTTATTAGGGACTTATTTGACACAAATCTAAAAGTATAGAGATTAAATTTGTAATTTAACTTTAAATTTAAAATGTTTAAGAGGTCACTCCAACATGATGAGGTGTAAGGGCATTATCTCGTAAGGTAAGAGGATAATAATTGTTAGAATCTAGAACAATCACCAATGCTTTATTGCTTGTGTAACAATCGGGTGGTGCCAAATTCCTTGCAAGTCAATGAGGAAGAGGAGTTTTTTTTGGGTGGAGTAATCCTTTTCTTATGTTTTAGTTTTAAAGTTCAGGGGTAGAAAGATCAACATGAGGTATTAAGTTCCTTTTGATGTTTAAAAAAGAAACTGGGATATATATTAATGAAAAGACCCATACCAAAAAGGGGAGATGAGTTATCTCTACATGATCAATGGGTTGAAAAGGATTTCAAATTGGAAAAGATATCCAATAGCTCAAAATTACAGAAATATTTAGAGGAAGATCAGCAAACGGAGGAGAAAAGCCTAGATACAAAAAACATTCATAGCAAGATTTAACCCTTGGAGGCCTGTGAGGTATTCATCCTGACGTTTAGAATTTGGTTTAGTTGTATGGGCACCTTTTGAAACTTATAAGAAACAATCTCTCGTTGCCTTTGTGGGGAATGACGAGTGGGTTCATGGGGAGAATATTTTCCTTCAAAGAGGCCCCCATTGTTCGTAGTTTCTACTTCATTCTATAGCCCTCTCGAAAGCTCTTTGATCTTCTCAAATATTCTTTCGAGGGGACCTCTTGTTTAATTTTTGCTGTTGTAGAGCTCTTAGTAGTCAAGAGATAGAGGACTTAGAGACTCCCTCTAATAACCAAAATTTCTATCCTAAATGTAGGGAGCGGTCGTTTGTGGTCACTTGAGCCTTTGGAAATCTTTTCTGATTTCTTTTGCGAGTCTTTTTTTTTCCCTCTCTGTGGACGCTTACAAACTTGAAGAAGATTATTCTTTTCTTGGCTCATAGCCTTGGAGAGGATTAATATCATAGAATTTTTGCTGATTCTTCGATTTTGTTCTTGACCCGAACTGGTGTGTCTAGTGTTTGTGTGATGTAGTCTTGGTAGTTGGTCCTTTTTTCTTAACCCTATGTAGTTAATTGTCTTTTACTGCCTTTCTTTTATTTGAATTAGTTTGCTTTTATTGACCAGCTTTTTACCATGTGGTAAATGGCTTTAGTTAGGCGGTTAAGTACATAAGACTTTATTAGGGTAACTTCCAGCTATATATATGGCTGGTGTTACTTTTTTTCAATTAATAAAAAAACTTTATAGAATCTTGAAGATTTGTGTGAGGGTACTAGTATGATATATTTGGGGAATAGAATTAGAAGGGTAGTAAGGGTATAATAGTAAATAGCTAGGGAGCCTTGGTTGTGTCTTGATTATAAATAGGGAAGTTAGGAAGCCTTAGAGGTGTGGAGAATTTTGGGTATAGTCTCATAGTGAGGCTTTGGGAGAGAGTTAGCCCTCTCGAAAGGCTATTGGTTATATTGTAACTTCTTTTGATATTGCAATATATAGTATTTGGTGTTTTCTTAGTTTGGATACCTAACAAATTGGTATCAGAGCACGTTCGTCCTAGAGCAAGCATCAACTGCAACCGCAATGGAGTTTGCTCGTTGGAACTACACTGGGAAACAATGCAGCACAAGATCAAGGAAATGCAACAAGGGATCAAGAAAGTGCAACAAATCTTAG

mRNA sequence

ATGTCAAAGTATGAATTTCCGAGGCTTCCACGGGCAGATATTGCATTGCTACTAGCACAGTGTCAAATTGCTGCTGTAACAGAGCGGGACCTTCTTCACCCGTCACCAGATTTGGTTTCAGATCTTTACACACGTTTAATGATCTACCTTGACTTGCTCCATGAGGAAGACCAAGGACAAGTTGAGTTTGCTGCCTTGGATCAGCTCGAGAACCCTGATCTGCATATGGATTCAGTTCGGATAATGAAGTTGCACAACAGAATAAAGCATGTCATTGCCTCTCTTGACTGTCCAAAAAAGTTTACATTGAAAGATTTGATAAAACCTGAAGGAGATAGGACCGAAATTTTCCTTAGTGCAATGTTGAACTTTTGCATTCACAAAGATGCCAAAATCACCCTCCATACTCCAGTTATGGATGAGCTGAGTACTCTTGGTGACCAGCAAAGGGAATGGGAGGTTAAGATTTCTCAGTTGAATGCAGAGATCGCAGAGTACAATGAAGCAAGAGAAAGGGAAATACCTTTTGTTCAAGAGATTGATGCTAAAGTGAAAGAACTACAACAGACTATTGGAGGACTTAACAATCAGCAGATGTCATTGCGTGCTTCTATCCGAAAGTTGAAAGACAAAGCTGGAGAAATGGATGAGAAGAGCACGTTCGTCCTAGAGCAAGCATCAACTGCAACCGCAATGGAGTTTGCTCGTTGGAACTACACTGGGAAACAATGCAGCACAAGATCAAGGAAATGCAACAAGGGATCAAGAAAGTGCAACAAATCTTAG

Coding sequence (CDS)

ATGTCAAAGTATGAATTTCCGAGGCTTCCACGGGCAGATATTGCATTGCTACTAGCACAGTGTCAAATTGCTGCTGTAACAGAGCGGGACCTTCTTCACCCGTCACCAGATTTGGTTTCAGATCTTTACACACGTTTAATGATCTACCTTGACTTGCTCCATGAGGAAGACCAAGGACAAGTTGAGTTTGCTGCCTTGGATCAGCTCGAGAACCCTGATCTGCATATGGATTCAGTTCGGATAATGAAGTTGCACAACAGAATAAAGCATGTCATTGCCTCTCTTGACTGTCCAAAAAAGTTTACATTGAAAGATTTGATAAAACCTGAAGGAGATAGGACCGAAATTTTCCTTAGTGCAATGTTGAACTTTTGCATTCACAAAGATGCCAAAATCACCCTCCATACTCCAGTTATGGATGAGCTGAGTACTCTTGGTGACCAGCAAAGGGAATGGGAGGTTAAGATTTCTCAGTTGAATGCAGAGATCGCAGAGTACAATGAAGCAAGAGAAAGGGAAATACCTTTTGTTCAAGAGATTGATGCTAAAGTGAAAGAACTACAACAGACTATTGGAGGACTTAACAATCAGCAGATGTCATTGCGTGCTTCTATCCGAAAGTTGAAAGACAAAGCTGGAGAAATGGATGAGAAGAGCACGTTCGTCCTAGAGCAAGCATCAACTGCAACCGCAATGGAGTTTGCTCGTTGGAACTACACTGGGAAACAATGCAGCACAAGATCAAGGAAATGCAACAAGGGATCAAGAAAGTGCAACAAATCTTAG

Protein sequence

MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQVEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSAMLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEIDAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKSTFVLEQASTATAMEFARWNYTGKQCSTRSRKCNKGSRKCNKS

Homology

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BLAST of Spg006211 vs. NCBI nr
Match: KAG6588803.1 (Kinetochore protein NUF2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 401.0 bits (1029), Expect = 8.0e-108
Identity = 203/229 (88.65%), Postives = 216/229 (94.32%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 58  MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 117

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+NR+KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 118 VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 177

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 178 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 237

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 238 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 286

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BLAST of Spg006211 vs. NCBI nr
Match: XP_022927739.1 (probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927740.1 probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927741.1 probable kinetochore protein NUF2 [Cucurbita moschata] >KAG7022576.1 NUF2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 401.0 bits (1029), Expect = 8.0e-108
Identity = 203/229 (88.65%), Postives = 216/229 (94.32%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+NR+KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

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BLAST of Spg006211 vs. NCBI nr
Match: XP_023529466.1 (probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo] >XP_023529467.1 probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo] >XP_023529468.1 probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.0 bits (1029), Expect = 8.0e-108
Identity = 203/229 (88.65%), Postives = 216/229 (94.32%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+NR+KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

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BLAST of Spg006211 vs. NCBI nr
Match: XP_022989268.1 (probable kinetochore protein NUF2 [Cucurbita maxima] >XP_022989269.1 probable kinetochore protein NUF2 [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 2.3e-107
Identity = 202/229 (88.21%), Postives = 215/229 (93.89%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADI LLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIVLLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+NR+KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

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BLAST of Spg006211 vs. NCBI nr
Match: XP_038877491.1 (kinetochore protein NUF2 homolog [Benincasa hispida] >XP_038877492.1 kinetochore protein NUF2 homolog [Benincasa hispida])

HSP 1 Score: 396.7 bits (1018), Expect = 1.5e-106
Identity = 205/229 (89.52%), Postives = 212/229 (92.58%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKY+FPRLPRADIALLLA  QIAAVTERDLLHPS DLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYDFPRLPRADIALLLADYQIAAVTERDLLHPSLDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAALDQLENPDLHMDSV  MKL N+IKHVIASLDCPKKFTLKDLIKPE DRTEIFLSA
Sbjct: 61  VEFAALDQLENPDLHMDSVSTMKLLNKIKHVIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVMDEL+T GDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMDELNTFGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

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BLAST of Spg006211 vs. ExPASy Swiss-Prot
Match: Q8RXJ0 (Kinetochore protein NUF2 homolog OS=Arabidopsis thaliana OX=3702 GN=NUF2 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.5e-61
Identity = 113/218 (51.83%), Postives = 166/218 (76.15%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MS YE+PRL R+DI   L   QIA+VTE DL  P+ D VS+LYTR++IYLD L EE++GQ
Sbjct: 1   MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRILIYLDALDEEEKGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           V+F AL+QLENPD H  S++ MKL+ ++K ++  LDCP   + KDL++PE  RTE F+SA
Sbjct: 61  VDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESSRTEFFISA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LN+ ++KD+K+ L  P  +EL  L +Q+++ E K++QLNAEI E++EA ER++PFVQE+
Sbjct: 121 LLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVERDLPFVQEL 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK 219
           +A +++L + I  LNNQQMSLRA+ +K+++K+ +MD +
Sbjct: 181 EANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNE 218

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BLAST of Spg006211 vs. ExPASy Swiss-Prot
Match: P33895 (Kinetochore protein NUF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=NUF2 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-06
Identity = 47/204 (23.04%), Postives = 96/204 (47.06%), Query Frame = 0


Query: 6   FPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLM-----IYLDLLHEEDQGQ 65
           FP L   ++ + L  C  A  T+ ++  P+ D +  LY +++     I ++ L      +
Sbjct: 8   FPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNSSNQE 67

Query: 66  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 125
                L Q EN ++++D++ ++ L N+I          + F + DL KPE  RT+  LSA
Sbjct: 68  TGDGHL-QEENENIYLDTLNVLVL-NKICFKFFENIGVQDFNMTDLYKPEAQRTQRLLSA 127

Query: 126 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQE- 185
           ++N+   ++ ++      + ++ +L  Q R      + +  ++ +Y +     IP  QE 
Sbjct: 128 VVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQEL 187

Query: 186 --IDAKVKELQQTIGGLNNQQMSL 202
             ++ + KEL+  +  L   Q +L
Sbjct: 188 QKLEEQNKELEIQLKKLTKIQETL 209

[Close]

BLAST of Spg006211 vs. ExPASy Swiss-Prot
Match: Q10173 (Kinetochore protein nuf2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nuf2 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.1e-06
Identity = 47/186 (25.27%), Postives = 93/186 (50.00%), Query Frame = 0


Query: 3   KYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLM-IYLDLLHEEDQGQV 62
           K+ FP L RA+I   +    I   T ++L  P+   V  LY   + +++ L  +  + +V
Sbjct: 4   KHTFPSLKRAEILECIDGLGI-PFTAKELDQPTSKAVIPLYEEFLDLFMGLTRQNLEEKV 63

Query: 63  EFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSAM 122
             +  D +EN ++  +S+R    +  +   + ++ C   FT++DL+KP+ +R ++ LSA+
Sbjct: 64  N-SLQDSVENFEIIHESLRFTVFYQILSQFMQNI-CFHDFTIQDLLKPDRNRLQLILSAV 123

Query: 123 LNFCIHK-------DAKITLHTPVMDELSTLGDQQREWEVKI-----SQLNAE-IAEYNE 175
           +NF   +       D  I     +++  + L  Q+++ E K+      +L +E I + NE
Sbjct: 124 INFAKLREERLQQFDDDIQKRESLLETYTLLDAQRKDLEEKVLLSQDRKLESEAIIKQNE 183

[Close]

BLAST of Spg006211 vs. ExPASy TrEMBL
Match: A0A6J1EIG6 (probable kinetochore protein NUF2 OS=Cucurbita moschata OX=3662 GN=LOC111434552 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.9e-108
Identity = 203/229 (88.65%), Postives = 216/229 (94.32%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+NR+KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

[Close]

BLAST of Spg006211 vs. ExPASy TrEMBL
Match: A0A6J1JNW1 (probable kinetochore protein NUF2 OS=Cucurbita maxima OX=3661 GN=LOC111486390 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.1e-107
Identity = 202/229 (88.21%), Postives = 215/229 (93.89%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADI LLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIVLLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+NR+KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

[Close]

BLAST of Spg006211 vs. ExPASy TrEMBL
Match: A0A1S4E5E4 (probable kinetochore protein NUF2 OS=Cucumis melo OX=3656 GN=LOC103503319 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 6.0e-101
Identity = 194/229 (84.72%), Postives = 206/229 (89.96%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLA  QIAAVTERDLL PSPDLVSDLYT LMIYLDLLHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           +EFAALDQLENPDLHM SV  MKLHN+IKH IASLDCPKKFTLKDLIKPE DRTEIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LNF IHKDAK+  H PVM+EL T  DQQREWEVKISQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKISQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQ+SLRA+IRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRAAIRKLKEKAGEMDEKISNAEFLLVQS 229

[Close]

BLAST of Spg006211 vs. ExPASy TrEMBL
Match: A0A6J1DWL1 (probable kinetochore protein NUF2 OS=Momordica charantia OX=3673 GN=LOC111024175 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 3.0e-100
Identity = 193/229 (84.28%), Postives = 208/229 (90.83%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKY   RLP ADIALLLAQ QIAAVTE DLLHPSPDLVSDLYTRL+IYLD LHEEDQGQ
Sbjct: 1   MSKYGLLRLPPADIALLLAQFQIAAVTEGDLLHPSPDLVSDLYTRLLIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAALDQLENPDLHMDSVRIMKL+ ++KHVIASLDC KKFTLKDLIKP+ DRTEIFLS 
Sbjct: 61  VEFAALDQLENPDLHMDSVRIMKLNLKLKHVIASLDCSKKFTLKDLIKPDTDRTEIFLST 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH P+++E++TLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQ+I
Sbjct: 121 MLNFCIHKDAKIVLHAPIINEVNTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQDI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL Q IGGLNNQQMSLRASIRKLK+K GEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQIIGGLNNQQMSLRASIRKLKEKVGEMDEKISNAEFLLVQS 229

[Close]

BLAST of Spg006211 vs. ExPASy TrEMBL
Match: A0A0A0KNL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G488740 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 5.1e-100
Identity = 193/229 (84.28%), Postives = 204/229 (89.08%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADI LLLA  QIAAVTERDLL PSPDLVSDLYT LMIYLDLLHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIVLLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           +EFAALDQLENPDLHM SV  MKLHN+IKH IASLDCPKKFTLKDLIKPE DRTEIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPETDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LNF IHKDAK+  H PVM+EL T  DQQREWEVK SQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKTSQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK---STFVLEQA 227
           DAKVKEL QTIGGLNNQQ+SLRASIRKLK+KAGEMDEK   + F+L Q+
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRASIRKLKEKAGEMDEKISNAEFLLVQS 229

[Close]

BLAST of Spg006211 vs. TAIR 10
Match: AT1G61000.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: mitosis; LOCATED IN: plasma membrane; EXPRESSED IN: cultured cell; CONTAINS InterPro DOMAIN/s: Kinetochore protein Nuf2 (InterPro:IPR005549); Has 50972 Blast hits to 29793 proteins in 2070 species: Archae - 902; Bacteria - 7404; Metazoa - 23628; Fungi - 4156; Plants - 2312; Viruses - 158; Other Eukaryotes - 12412 (source: NCBI BLink). )

HSP 1 Score: 236.1 bits (601), Expect = 3.2e-62
Identity = 113/218 (51.83%), Postives = 166/218 (76.15%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MS YE+PRL R+DI   L   QIA+VTE DL  P+ D VS+LYTR++IYLD L EE++GQ
Sbjct: 1   MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRILIYLDALDEEEKGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNRIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           V+F AL+QLENPD H  S++ MKL+ ++K ++  LDCP   + KDL++PE  RTE F+SA
Sbjct: 61  VDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESSRTEFFISA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LN+ ++KD+K+ L  P  +EL  L +Q+++ E K++QLNAEI E++EA ER++PFVQE+
Sbjct: 121 LLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVERDLPFVQEL 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEK 219
           +A +++L + I  LNNQQMSLRA+ +K+++K+ +MD +
Sbjct: 181 EANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNE 218

The following BLAST results are available for this feature:

BLAST of Spg006211 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6588803.1	8.0e-108	88.65	Kinetochore protein NUF2-like protein, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022927739.1	8.0e-108	88.65	probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927740.1 probable ...	[more]
XP_023529466.1	8.0e-108	88.65	probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo] >XP_023529467.1 p...	[more]
XP_022989268.1	2.3e-107	88.21	probable kinetochore protein NUF2 [Cucurbita maxima] >XP_022989269.1 probable ki...	[more]
XP_038877491.1	1.5e-106	89.52	kinetochore protein NUF2 homolog [Benincasa hispida] >XP_038877492.1 kinetochore...	[more]

BLAST of Spg006211 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXJ0	4.5e-61	51.83	Kinetochore protein NUF2 homolog OS=Arabidopsis thaliana OX=3702 GN=NUF2 PE=1 SV...	[more]
P33895	1.4e-06	23.04	Kinetochore protein NUF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
Q10173	4.1e-06	25.27	Kinetochore protein nuf2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]

BLAST of Spg006211 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EIG6	3.9e-108	88.65	probable kinetochore protein NUF2 OS=Cucurbita moschata OX=3662 GN=LOC111434552 ...	[more]
A0A6J1JNW1	1.1e-107	88.21	probable kinetochore protein NUF2 OS=Cucurbita maxima OX=3661 GN=LOC111486390 PE...	[more]
A0A1S4E5E4	6.0e-101	84.72	probable kinetochore protein NUF2 OS=Cucumis melo OX=3656 GN=LOC103503319 PE=3 S...	[more]
A0A6J1DWL1	3.0e-100	84.28	probable kinetochore protein NUF2 OS=Momordica charantia OX=3673 GN=LOC111024175...	[more]
A0A0A0KNL7	5.1e-100	84.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G488740 PE=3 SV=1	[more]

BLAST of Spg006211 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61000.1	3.2e-62	51.83	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: mitosis; LOCATED IN: plas...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 177..197
None	No IPR available	COILS	Coil	Coil	coord: 149..169
None	No IPR available	PANTHER	PTHR21650	MEMBRALIN/KINETOCHORE PROTEIN NUF2	coord: 1..220
None	No IPR available	PANTHER	PTHR21650:SF2	KINETOCHORE PROTEIN NUF2	coord: 1..220
IPR038275	Nuf2, N-terminal domain superfamily	GENE3D	1.10.418.60	Ncd80 complex, Nuf2 subunit	coord: 1..164 e-value: 1.9E-36 score: 127.5
IPR005549	Kinetochore protein Nuf2, N-terminal	PFAM	PF03800	Nuf2	coord: 3..141 e-value: 3.8E-28 score: 98.4

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg006211.1	Spg006211.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051315	attachment of mitotic spindle microtubules to kinetochore
biological_process	GO:0051301	cell division
biological_process	GO:0051383	kinetochore organization
biological_process	GO:0045132	meiotic chromosome segregation
biological_process	GO:0007052	mitotic spindle organization
cellular_component	GO:0031262	Ndc80 complex
cellular_component	GO:0000776	kinetochore
molecular_function	GO:0044877	protein-containing complex binding