Spg006161 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006161
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionnudix hydrolase 7-like
Locationscaffold4: 4848231 .. 4852046 (-)
RNA-Seq ExpressionSpg006161
SyntenySpg006161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAAATTCAATCAATTCATTGAAGAACGAATCAACGCGGCGACTGTACTGTCTACTCTTCAAATTTGCCGTTCTATTTCCTTCAATTTTCACACATTCAATGGAAGCTTCAAAGTCCTTGAACCCGATTTCAAGGCAATGGTATAGTTCTTCATCGTTCTTATAAATCTTCACCATCGATTCGACCTGTTCTCCACGTCCATTCTGGTGTTGGGTTTGATTTTTCCGCAGGGAGATTAGTTTATTGGTTGATTTTTCTTCATGTTGAGGCTGAAGAGTTATAAATTATACCCTTATCTTTCTTTATTTAAAGCGTTTCGTTCTCCATTGGCCTCCTCTGCACTTCATCCCTTATCGAATCCAGCTTCCTTGAACTCTTCCTCGTCTTCCCTTTTCGGCTTCAGAACGCGTATGAGATTCTTTTATCTGTTTTCTTCTTGTTTTTGTGCGTCTTTGAGCTGATTCTATGTGGGTCTTTCGTGGGGTTTCTGATTTATTGCTGTAATTTAATGGGTTCTTTCTCGATTGCTGTACTGAAGTCCCAATTCCGTTTCTCCAATCGAGGAGAAAATTGATTGGGATTTGATGAAATGGATTTGTTTGGGTTGAAATTTGTGGACTTATTCTGGGCTCCTTTTGGGGGCTTTTTCTTGTTTGTCATTCTTGCAGCTGCGGCTACTTTTTCATACCTAACAAACAAAAGTCTAACCCCTAGCCGAGGCATGTCTTCTTCATCAACTTCATTGGCAATTGCAGAGAAAGAGATTCCTCAGGATAATATTGAACAGCTTGAATTATTGAGTGGAGTTTATGATCATTATGAAGGAGTTATTGTAGAGATGAAGGATCCTATGGATCCAAAAGAATTTGGTTTTTTGCTTAAAGCTTCATTATCTCAATGGAAGCAGCTGGTATTTGATCTCTGTCCTGTTATAGGTTGAACATGGTTCTTTGTACTGGTTTTCAACTCTCATTTATGGTTTCTGTTTGCATTTTGGAATTGCAGGGTAAGAGGGGTATTTGGATCAAATTGCCTATTGAACTTGCAAATCTTGTTGAAGTTGTTGTTAAGGTACATTTTGTTCATACACAAAAGAAGTTGTGCTTAAAAACTTATATGCTATCTGTGTTAAAAATGTAATAGCATGGTACATGCAGTAATATTAAAATTAAGATGAAGGGTATAAAGAAGTCTAGTCATTACAACCCCAATATGTTAAGTACACTGAATGATTGAAAAAGACATTTGTTCCATTGAGCGGTATCTTTTGAATTTCAACATAATTCTACCTTTAAACTTTCAAAAGCTTACAATATGCCTTTTGATTGTTTCAAAATCATTCGTACAATAACCATAATGAACAGAAGTATATACTGATAATTCACGTTGGGCAAAATGGATACCTCATATACAGTTCAAGAGCAAAATATAAGCGTACATATAGATGTTGCTTTATGTTTCTATTAAAAATGGCGTTGACCTGAAGGGTAATTTTGAACCAATTGAGAAGTTTGAAAGGTATATTTGAAACTTTTCAAAGTTTAGGGATAGAGTTGAAAATTCATATTAAGTCTAGGGGCAAAAACTATAATTGAGCCTTAAGTTTATTTCACTTTTGTTTTTTATTTTATATCCATGAATATTTAGGCCATGAGAACTTGACCAATAATATTTGGTTGTCAAGAAACTGTTAGGATTTGATCCTAGGTGGATAGCCATCATAGGGTTTGAACCAATTACTCTCAAATTCCCTTACTTTTACCCACATGTCCCGATGTCACTAGGTCTACCTATGGTGGTTTAATTTAGCCTAAAGTTCAAATACCCTCGTTGTTTGTATCTGCTTGAGAAAATGGAGATTTTCCAAGTCACTTTCTCATAATAGAGTCCTCCTTGTTTTGTTTGATTTTCAGGAAGGATTTGTCTTCCACCATGCTGAACCCAATTACCTAATGCTTGTGCGATGGATCTCTGAAACTACCAATAATCTCCCTCCTAATGCCTCTCATCGCGTCGGCATTGGTGCATTCGTAATGAATAGCAATCGAGAGGTACTTTCCAAACTAATGCTGTTTGTTTGTGATTTGAAAAACAATACTATGTTCATAATCTTCTATCTATTTGAAAGGTTTGACTAAGAAGAGCTCTTTGTTCCTAGTAACTAACATTAATACTCTCATTCTGAAGGTGCTTGTGGTTCAAGAGATCAGTGGCAAATTCAAAGGTTCAGGAGTTTGGAAGTTACCAACTGGAGTGGTTAACGAGGTGAGCAAAGTTCGATTTGCTGCAATTGTCTCGATTCGACAAACCTTCTTATGCTATAATGTACTTACCCTTTCTGTAGGGAGAGGATATTTGTGAAGCAGCAATTAGAGAAGTAAAAGAAGAGACTGGTGTAAGTAAAAATAACAAGAAGCAAGCAGTTGCATTTTTTAAAAAAATTCACACACTCTCTCTTTCTCTCCCAAATGTTTAACAGTTCATTTGAATTTCTTTCAGGTTGATGCAGAATTTGTAGAAGTGTTAGCATTCAGGTCTGAAATCTCTACCCCTCAAAATAACTGAAATCATTATGCTCAAGTTACATTCTTTCATATTTCCTTCTCATTTTTCACAGTACACCAATATTTTTTCATACTGGTGAAAGAGGGTTCTCTTTCTTACCATTTTTTTTATTTAATCTGGATTTCTTACCATCTGCTCCTTTCTTGGGTAATGCAGGCAAAGCCACAGAGCATTCTTTACGAAATCGGATTTGTTTTTCGTATGCATGTTACAACCGTGCTCCTTCGACATCCAAAAGCAAGTTTCAGAAATTGAGGCAGCCAAGGTATATTTTAGTAGACCATGATTGATAATTGATGAGTTATGCCATTTTTTTTTTTATTTTGAAGTAAAATTCCTCCTTGATCTATTAATGAGAGCCCCAAATTTGGAATCCAATGTTTCCATATCAATGATTGATCAATAATCTAAGCTTTAGGACAGGTCTCTGTTGTCTAGATTAGTTGTCTTATAAAACTGTCAATTAAAAACACTTATCCACGTATACGATACTAGCTCGGCCTCGACCTTTTGAATATCAAACGTTTATATGCATCAAAACATTTAAGACTAGATTGTGGTGATAATTTATTTTCCCATTTCTGACTTCTTCCCCTCCCCCCAACAAACAGTGGATGCCCATTGATGAATATGCAGATCAACCCTTTGTTAAAAATAACTCGGGTTTCGACTACGTGGCAAAAGTATGTCTAGCGAAAGCGAGTGACAACTATACCGGCCTCTCGGCGAAGCCTACATATTCTGCCTCTGGTAAAATGACTTACCTCTATTCAAAACTGGAAACTTGAACTGACATTGCAGTTGCTGGGTAGAGGCTAACCACTTCAACAGCCACAATGATAGCTCCCTCTAATCTCTGCATTCCAAGAAGCTGAAAGAAAATCTGTGAAGTCTGTGTGTACAGATCATTTGTACCTTAAAGCCATCTTTTCTCATTCGGTTTTCGGGTTCGAGAAAATTTCTAGTACTAGTGATTTGTTGATTAATAGTTAAGTCACATGATTTATAATTGCATTTTATAAATGTTTTATAAATGTTGGTAATAAGGTGAAGGGTTAATAACATAGTACTGAATGGTATTATAAAATAATGTACTGTGGAACACTTCAAGTCCCTTTTTAATTGTAATGCCAACTATTTGTCAGAGTTCAAATAATGATGTGAGTTCCTTGAGTCTTGTTTGTTTTCTTCTTTTAGACTGTAAAATTCTTGGAGTTTGATCTTTTTTTTCTTTTCTTTTTTTAAATG

mRNA sequence

ATGGATTTGTTTGGGTTGAAATTTGTGGACTTATTCTGGGCTCCTTTTGGGGGCTTTTTCTTGTTTGTCATTCTTGCAGCTGCGGCTACTTTTTCATACCTAACAAACAAAAGTCTAACCCCTAGCCGAGGCATGTCTTCTTCATCAACTTCATTGGCAATTGCAGAGAAAGAGATTCCTCAGGATAATATTGAACAGCTTGAATTATTGAGTGGAGTTTATGATCATTATGAAGGAGTTATTGTAGAGATGAAGGATCCTATGGATCCAAAAGAATTTGGTTTTTTGCTTAAAGCTTCATTATCTCAATGGAAGCAGCTGGGTAAGAGGGGTATTTGGATCAAATTGCCTATTGAACTTGCAAATCTTGTTGAAGTTGTTGTTAAGGAAGGATTTGTCTTCCACCATGCTGAACCCAATTACCTAATGCTTGTGCGATGGATCTCTGAAACTACCAATAATCTCCCTCCTAATGCCTCTCATCGCGTCGGCATTGGTGCATTCGTAATGAATAGCAATCGAGAGGTGCTTGTGGTTCAAGAGATCAGTGGCAAATTCAAAGGTTCAGGAGTTTGGAAGTTACCAACTGGAGTGGTTAACGAGGGAGAGGATATTTGTGAAGCAGCAATTAGAGAAGTAAAAGAAGAGACTGGTGTTGATGCAGAATTTGTAGAAGTGTTAGCATTCAGGCAAAGCCACAGAGCATTCTTTACGAAATCGGATTTGTTTTTCGTATGCATGTTACAACCGTGCTCCTTCGACATCCAAAAGCAAGTTTCAGAAATTGAGGCAGCCAAGTGGATGCCCATTGATGAATATGCAGATCAACCCTTTGTTAAAAATAACTCGGGTTTCGACTACGTGGCAAAAGTATGTCTAGCGAAAGCGAGTGACAACTATACCGGCCTCTCGGCGAAGCCTACATATTCTGCCTCTGGTAAAATGACTTACCTCTATTCAAAACTGGAAACTTGA

Coding sequence (CDS)

ATGGATTTGTTTGGGTTGAAATTTGTGGACTTATTCTGGGCTCCTTTTGGGGGCTTTTTCTTGTTTGTCATTCTTGCAGCTGCGGCTACTTTTTCATACCTAACAAACAAAAGTCTAACCCCTAGCCGAGGCATGTCTTCTTCATCAACTTCATTGGCAATTGCAGAGAAAGAGATTCCTCAGGATAATATTGAACAGCTTGAATTATTGAGTGGAGTTTATGATCATTATGAAGGAGTTATTGTAGAGATGAAGGATCCTATGGATCCAAAAGAATTTGGTTTTTTGCTTAAAGCTTCATTATCTCAATGGAAGCAGCTGGGTAAGAGGGGTATTTGGATCAAATTGCCTATTGAACTTGCAAATCTTGTTGAAGTTGTTGTTAAGGAAGGATTTGTCTTCCACCATGCTGAACCCAATTACCTAATGCTTGTGCGATGGATCTCTGAAACTACCAATAATCTCCCTCCTAATGCCTCTCATCGCGTCGGCATTGGTGCATTCGTAATGAATAGCAATCGAGAGGTGCTTGTGGTTCAAGAGATCAGTGGCAAATTCAAAGGTTCAGGAGTTTGGAAGTTACCAACTGGAGTGGTTAACGAGGGAGAGGATATTTGTGAAGCAGCAATTAGAGAAGTAAAAGAAGAGACTGGTGTTGATGCAGAATTTGTAGAAGTGTTAGCATTCAGGCAAAGCCACAGAGCATTCTTTACGAAATCGGATTTGTTTTTCGTATGCATGTTACAACCGTGCTCCTTCGACATCCAAAAGCAAGTTTCAGAAATTGAGGCAGCCAAGTGGATGCCCATTGATGAATATGCAGATCAACCCTTTGTTAAAAATAACTCGGGTTTCGACTACGTGGCAAAAGTATGTCTAGCGAAAGCGAGTGACAACTATACCGGCCTCTCGGCGAAGCCTACATATTCTGCCTCTGGTAAAATGACTTACCTCTATTCAAAACTGGAAACTTGA

Protein sequence

MDLFGLKFVDLFWAPFGGFFLFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYSKLET
Homology
BLAST of Spg006161 vs. NCBI nr
Match: KAG7022790.1 (Nudix hydrolase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 519.6 bits (1337), Expect = 1.9e-143
Identity = 263/303 (86.80%), Postives = 282/303 (93.07%), Query Frame = 0

Query: 21  LFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGV 80
           L  I+  AA  SYL+NK+ + SRGMSSSSTSLAI+E E P DNIEQLELL GVYD+YEGV
Sbjct: 44  LLGIITLAA--SYLSNKTPSLSRGMSSSSTSLAISENETPLDNIEQLELLPGVYDNYEGV 103

Query: 81  IVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPN 140
           IVEMKDPMDP EFG LL+ASLSQWKQLGKRGIWIKLPI+LANL+EVVVKEGFVFHHAEP+
Sbjct: 104 IVEMKDPMDPNEFGSLLRASLSQWKQLGKRGIWIKLPIKLANLIEVVVKEGFVFHHAEPD 163

Query: 141 YLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVN 200
           YLMLVRWISETTN++P NASHRVGIGAFVMNSNREVLVVQEISGKFKG+GVWKLPTGVVN
Sbjct: 164 YLMLVRWISETTNSIPANASHRVGIGAFVMNSNREVLVVQEISGKFKGTGVWKLPTGVVN 223

Query: 201 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVS 260
           EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHR+FFTKSDLFFVCMLQP SF +QKQ S
Sbjct: 224 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRSFFTKSDLFFVCMLQPRSFAVQKQNS 283

Query: 261 EIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYS 320
           EIEAAKWMPIDEYADQPFVK NSGFDYVAKVCLAKA++NYTGLSA PTYS+SGK TYLYS
Sbjct: 284 EIEAAKWMPIDEYADQPFVKKNSGFDYVAKVCLAKANNNYTGLSAIPTYSSSGKKTYLYS 343

Query: 321 KLE 324
           KL+
Sbjct: 344 KLD 344

BLAST of Spg006161 vs. NCBI nr
Match: XP_023529751.1 (nudix hydrolase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.8 bits (1335), Expect = 3.3e-143
Identity = 262/303 (86.47%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 21  LFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGV 80
           L  I+  AA  SYL+NK+ + SRGM SSSTSLAIAE E P DNIEQLELL GVYD+YEGV
Sbjct: 44  LLGIITLAA--SYLSNKTPSLSRGMPSSSTSLAIAENETPLDNIEQLELLPGVYDNYEGV 103

Query: 81  IVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPN 140
           IVEMKDPMDP EFG+LL+ASLSQWKQLGKRGIWIKLPI+LANL+EVVVKEGFVFHHAEP+
Sbjct: 104 IVEMKDPMDPDEFGYLLRASLSQWKQLGKRGIWIKLPIKLANLIEVVVKEGFVFHHAEPD 163

Query: 141 YLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVN 200
           YLMLVRWISETTN++P NASHRVGIGAFVMNSNREVLVVQEISGKFKG+GVWK PTGVVN
Sbjct: 164 YLMLVRWISETTNSIPANASHRVGIGAFVMNSNREVLVVQEISGKFKGTGVWKFPTGVVN 223

Query: 201 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVS 260
           EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHR+FFTKSDLFFVCMLQP SF +QKQ S
Sbjct: 224 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRSFFTKSDLFFVCMLQPRSFAVQKQNS 283

Query: 261 EIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYS 320
           EIEAAKWMPIDEYADQPFVK NSGFDYVAKVCLAKA++NYTGLSA PTYS+SGK TYLYS
Sbjct: 284 EIEAAKWMPIDEYADQPFVKKNSGFDYVAKVCLAKANNNYTGLSAIPTYSSSGKKTYLYS 343

Query: 321 KLE 324
           KL+
Sbjct: 344 KLD 344

BLAST of Spg006161 vs. NCBI nr
Match: XP_022136267.1 (nudix hydrolase 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 516.5 bits (1329), Expect = 1.6e-142
Identity = 255/295 (86.44%), Postives = 280/295 (94.92%), Query Frame = 0

Query: 29  ATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPM 88
           A++SYLTNK L+ S+GMSSSSTSLA AE+EI QDNIEQLELLSGV+DHY GVIVEMKDP+
Sbjct: 50  ASYSYLTNKRLSLSQGMSSSSTSLASAEEEIRQDNIEQLELLSGVHDHYGGVIVEMKDPI 109

Query: 89  DPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 148
           DPKEFG LL+ASLSQW+QLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI
Sbjct: 110 DPKEFGSLLRASLSQWRQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 169

Query: 149 SETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEA 208
           SETTN+LP NASHRVGIGAFVMN+NREVLVVQEI GKFKGSGVWKLPTGVVNEGEDICEA
Sbjct: 170 SETTNSLPHNASHRVGIGAFVMNTNREVLVVQEIGGKFKGSGVWKLPTGVVNEGEDICEA 229

Query: 209 AIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWM 268
           AIREVKEET +DAEFVEVLAFRQSH +FFTKSDLFFVCML+P S+DIQKQ SEIEAAKWM
Sbjct: 230 AIREVKEETDIDAEFVEVLAFRQSHNSFFTKSDLFFVCMLRPSSYDIQKQASEIEAAKWM 289

Query: 269 PIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYSKLE 324
           PIDEYADQPFV+ NS FDYVAKVC+AKA+++YTGLSA PT+S+SGK++YLYSKL+
Sbjct: 290 PIDEYADQPFVRANSAFDYVAKVCVAKANNDYTGLSANPTFSSSGKLSYLYSKLK 344

BLAST of Spg006161 vs. NCBI nr
Match: XP_022989436.1 (nudix hydrolase 7-like isoform X1 [Cucurbita maxima] >XP_022989437.1 nudix hydrolase 7-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 516.5 bits (1329), Expect = 1.6e-142
Identity = 263/303 (86.80%), Postives = 282/303 (93.07%), Query Frame = 0

Query: 21  LFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGV 80
           L  I+  AA  SYL+NK+ + SRGMSSSSTSLAIAEKE P DNIEQLELL GVYD+YEGV
Sbjct: 44  LLGIITLAA--SYLSNKTPSLSRGMSSSSTSLAIAEKETPLDNIEQLELLPGVYDNYEGV 103

Query: 81  IVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPN 140
           IVEMKDPMDP EFG LL+ASLSQWKQLGKRGIWIKLPI+LANL+EVVVKEGFVFHHAEP+
Sbjct: 104 IVEMKDPMDPNEFGSLLRASLSQWKQLGKRGIWIKLPIKLANLIEVVVKEGFVFHHAEPD 163

Query: 141 YLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVN 200
           YLMLVRW+SETTN++P NASHRVGIGAFVMNSNREVLVVQEISGKFKG+GVWKLPTGVVN
Sbjct: 164 YLMLVRWVSETTNSIPANASHRVGIGAFVMNSNREVLVVQEISGKFKGTGVWKLPTGVVN 223

Query: 201 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVS 260
           EGEDICEAAIREVKEETGVDAEFVEVLAFR+SHR+FFTKSDLFFVCMLQP SF +QKQ S
Sbjct: 224 EGEDICEAAIREVKEETGVDAEFVEVLAFRESHRSFFTKSDLFFVCMLQPRSFAVQKQNS 283

Query: 261 EIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYS 320
           EIEAAKWMPIDEYADQPFVK NSGFDYVAKVCLAK S+NYTGLSA PTYS+SGK TYLYS
Sbjct: 284 EIEAAKWMPIDEYADQPFVKKNSGFDYVAKVCLAK-SNNYTGLSAIPTYSSSGKKTYLYS 343

Query: 321 KLE 324
           KL+
Sbjct: 344 KLD 343

BLAST of Spg006161 vs. NCBI nr
Match: XP_022136268.1 (nudix hydrolase 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 516.5 bits (1329), Expect = 1.6e-142
Identity = 255/295 (86.44%), Postives = 280/295 (94.92%), Query Frame = 0

Query: 29  ATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPM 88
           A++SYLTNK L+ S+GMSSSSTSLA AE+EI QDNIEQLELLSGV+DHY GVIVEMKDP+
Sbjct: 30  ASYSYLTNKRLSLSQGMSSSSTSLASAEEEIRQDNIEQLELLSGVHDHYGGVIVEMKDPI 89

Query: 89  DPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 148
           DPKEFG LL+ASLSQW+QLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI
Sbjct: 90  DPKEFGSLLRASLSQWRQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 149

Query: 149 SETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEA 208
           SETTN+LP NASHRVGIGAFVMN+NREVLVVQEI GKFKGSGVWKLPTGVVNEGEDICEA
Sbjct: 150 SETTNSLPHNASHRVGIGAFVMNTNREVLVVQEIGGKFKGSGVWKLPTGVVNEGEDICEA 209

Query: 209 AIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWM 268
           AIREVKEET +DAEFVEVLAFRQSH +FFTKSDLFFVCML+P S+DIQKQ SEIEAAKWM
Sbjct: 210 AIREVKEETDIDAEFVEVLAFRQSHNSFFTKSDLFFVCMLRPSSYDIQKQASEIEAAKWM 269

Query: 269 PIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYSKLE 324
           PIDEYADQPFV+ NS FDYVAKVC+AKA+++YTGLSA PT+S+SGK++YLYSKL+
Sbjct: 270 PIDEYADQPFVRANSAFDYVAKVCVAKANNDYTGLSANPTFSSSGKLSYLYSKLK 324

BLAST of Spg006161 vs. ExPASy Swiss-Prot
Match: Q94B74 (Nudix hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=NUDT2 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 9.7e-82
Identity = 151/260 (58.08%), Postives = 187/260 (71.92%), Query Frame = 0

Query: 45  MSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQW 104
           MS+SS+S        P    +   LL  V D Y GV+ EM  PMDP  F  LL++SLS W
Sbjct: 1   MSASSSSTN------PMSREDATTLLPSVQDKYGGVMTEMTHPMDPSLFSTLLRSSLSTW 60

Query: 105 KQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVG 164
              GK+G+WIKLP +L  L E  VKEGF FHHAE +YLMLV WI +  + LP NASHRVG
Sbjct: 61  TLQGKKGVWIKLPKQLIGLAETAVKEGFWFHHAEKDYLMLVYWIPKEDDTLPANASHRVG 120

Query: 165 IGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFV 224
           IGAFV+N N+EVLVVQE +G+F+G G+WK PTGVVNEGEDI + ++REVKEETGVD EF 
Sbjct: 121 IGAFVINHNKEVLVVQEKTGRFQGQGIWKFPTGVVNEGEDIHDGSVREVKEETGVDTEFD 180

Query: 225 EVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSG 284
           ++LAFRQ+H+AFF KSDLFFVCML+P S +I  Q SEIEAA+WMP +EY +QPFV+N   
Sbjct: 181 QILAFRQTHKAFFGKSDLFFVCMLKPLSLEINAQESEIEAAQWMPWEEYINQPFVQNYEL 240

Query: 285 FDYVAKVCLAKASDNYTGLS 305
             Y+  +C AK + +Y G +
Sbjct: 241 LRYMTDICSAKTNGDYEGFT 254

BLAST of Spg006161 vs. ExPASy Swiss-Prot
Match: Q9SU14 (Nudix hydrolase 7 OS=Arabidopsis thaliana OX=3702 GN=NUDT7 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.7e-81
Identity = 150/257 (58.37%), Postives = 188/257 (73.15%), Query Frame = 0

Query: 65  EQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLV 124
           +Q+ LL G  D+Y+GV V M +PMD + F   L+ASLS W++ GK+GIWIKLP+ LANLV
Sbjct: 6   QQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLV 65

Query: 125 EVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSN-REVLVVQEIS 184
           E  V EGF +HHAEP YLMLV WISET + +P NASH VG GA V+N N +EVLVVQE S
Sbjct: 66  EAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERS 125

Query: 185 GKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-TKSDL 244
           G FK   VWKLPTGV+NEGEDI     REV+EETG+ A+FVEVLAFRQSH+A    K+D+
Sbjct: 126 GFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDM 185

Query: 245 FFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTG 304
           FF+C+L P S+DI +Q SEI  AKWMPI EY DQP+ K N  F ++A +C  K  + Y G
Sbjct: 186 FFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLG 245

Query: 305 LSAKPTYSASGKMTYLY 320
            +  PT ++SGK +++Y
Sbjct: 246 FAIVPTTTSSGKESFIY 262

BLAST of Spg006161 vs. ExPASy Swiss-Prot
Match: Q6NPD7 (Nudix hydrolase 10 OS=Arabidopsis thaliana OX=3702 GN=NUDT10 PE=1 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 9.7e-74
Identity = 144/270 (53.33%), Postives = 183/270 (67.78%), Query Frame = 0

Query: 54  IAEKEIP-QDNIEQ--LELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKR 113
           ++++E P ++ +E    E+L  V D Y GVIVEMK PMD K F   L+ S  QW+  GK+
Sbjct: 1   MSDQEAPLRNGVEHKIFEVLPFVDDDYGGVIVEMKTPMDTKNFVAALRDSFEQWRLQGKK 60

Query: 114 GIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVM 173
           G+W+ LP+   NLVE  VKEGF +HHAEP YLMLV WI E  + +P NASHRV +GA V+
Sbjct: 61  GVWLNLPLSHVNLVEPAVKEGFRYHHAEPTYLMLVYWIPEAESTIPLNASHRVRVGAVVL 120

Query: 174 NSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFR 233
           N N+E     E  G   GSG+WK+PTGVV+EGE+I  AAIREVKEETG+D EF+E+LAF 
Sbjct: 121 NHNKE-----EKYGSLCGSGIWKIPTGVVDEGEEIFAAAIREVKEETGIDTEFLEILAFC 180

Query: 234 QSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAK 293
           Q+H +FF KSDLFFVC+L+P SFDIQKQ  EIEAA+WM  ++ A QP    N  F  +  
Sbjct: 181 QTHESFFAKSDLFFVCLLRPTSFDIQKQDLEIEAAQWMRFEDSASQPITHKNDLFKDIHH 240

Query: 294 VCLAKASDNYTGLSAKP-TYSASGKMTYLY 320
           +C  K   +Y+G S KP T     K+ YLY
Sbjct: 241 ICSMKMEKSYSGFSKKPITTFFDDKLGYLY 265

BLAST of Spg006161 vs. ExPASy Swiss-Prot
Match: Q9SJC4 (Nudix hydrolase 6 OS=Arabidopsis thaliana OX=3702 GN=NUDT6 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.0e-72
Identity = 141/262 (53.82%), Postives = 185/262 (70.61%), Query Frame = 0

Query: 62  DNIEQLE-LLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIEL 121
           DN +Q   LL GV D+Y GV V + +PM  ++F   L+ASL  W   G +GIW+KL   L
Sbjct: 2   DNEDQESLLLQGVPDNYGGVKVNLTEPMTIEDFVPKLRASLVYWSNQGTKGIWLKLADGL 61

Query: 122 ANLVEVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMN-SNREVLVV 181
            NL+     EGFV HHAE  Y ML  WI++  + LP NASHR+G+GAFV+N   +EVLVV
Sbjct: 62  DNLIAPAKAEGFVCHHAEREYTMLTSWIADVPSTLPANASHRIGVGAFVLNKKTKEVLVV 121

Query: 182 QEISGKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-T 241
           QEI G FKG+GVWKLPTGVV EGE+I E A+REV+EETG+  +FVEVLAFR+SH+AF   
Sbjct: 122 QEIDGHFKGTGVWKLPTGVVKEGENIWEGALREVEEETGIKTKFVEVLAFRESHQAFLEI 181

Query: 242 KSDLFFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASD 301
           K+D+FF+C L+P +F+I+KQ SEI AAKWMPI+EY +QP+ +    F ++A +CL +  +
Sbjct: 182 KTDIFFLCELEPTTFEIKKQDSEILAAKWMPIEEYVNQPWNQKKELFRFMANICLKRLQE 241

Query: 302 -NYTGLSAKPTYSASGKMTYLY 320
             Y G S   T ++SGK +YLY
Sbjct: 242 MEYMGFSKVLTTTSSGKESYLY 263

BLAST of Spg006161 vs. ExPASy Swiss-Prot
Match: Q9SJC6 (Nudix hydrolase 5 OS=Arabidopsis thaliana OX=3702 GN=NUDT5 PE=2 SV=3)

HSP 1 Score: 259.2 bits (661), Expect = 6.1e-68
Identity = 126/242 (52.07%), Postives = 169/242 (69.83%), Query Frame = 0

Query: 66  QLELLSGVYDHYEGVIVEMK--DPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANL 125
           ++ LL G  D + G +V +   + M   +F   L  SL  WK  GK+GIWIKLP EL++L
Sbjct: 26  EISLLDGEEDRFGGTVVNLMEVESMTIGDFDSKLDVSLKAWKDQGKKGIWIKLPSELSSL 85

Query: 126 VEVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEIS 185
           V+  +K+GF +HHAE  Y+ML  W+ E  + LP NASHR+GIGAFV+N N E+LVVQE S
Sbjct: 86  VDTAIKKGFTYHHAENEYVMLTFWLPEPPSTLPCNASHRIGIGAFVLNKNGEMLVVQENS 145

Query: 186 GKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-TKSDL 245
           G FK   VWK+PTG + EGE I   A+REVKEET +DAEFVEVL+F +SH+A +  K+D+
Sbjct: 146 GYFKDKNVWKVPTGTIKEGESIWAGAVREVKEETDIDAEFVEVLSFMESHQAVWQRKTDI 205

Query: 246 FFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPF--VKNNSGFDYVAKVCLAKASDNY 303
           FFVC L+  +F+IQKQ SEI AAKWMP++EY +QP+   + N  F  +A +CL ++ + Y
Sbjct: 206 FFVCELEARTFEIQKQDSEIHAAKWMPVEEYVNQPYHNKEGNEMFKLIANICLKRSREKY 265

BLAST of Spg006161 vs. ExPASy TrEMBL
Match: A0A6J1JK23 (nudix hydrolase 7-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486492 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.8e-143
Identity = 263/303 (86.80%), Postives = 282/303 (93.07%), Query Frame = 0

Query: 21  LFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGV 80
           L  I+  AA  SYL+NK+ + SRGMSSSSTSLAIAEKE P DNIEQLELL GVYD+YEGV
Sbjct: 44  LLGIITLAA--SYLSNKTPSLSRGMSSSSTSLAIAEKETPLDNIEQLELLPGVYDNYEGV 103

Query: 81  IVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPN 140
           IVEMKDPMDP EFG LL+ASLSQWKQLGKRGIWIKLPI+LANL+EVVVKEGFVFHHAEP+
Sbjct: 104 IVEMKDPMDPNEFGSLLRASLSQWKQLGKRGIWIKLPIKLANLIEVVVKEGFVFHHAEPD 163

Query: 141 YLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVN 200
           YLMLVRW+SETTN++P NASHRVGIGAFVMNSNREVLVVQEISGKFKG+GVWKLPTGVVN
Sbjct: 164 YLMLVRWVSETTNSIPANASHRVGIGAFVMNSNREVLVVQEISGKFKGTGVWKLPTGVVN 223

Query: 201 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVS 260
           EGEDICEAAIREVKEETGVDAEFVEVLAFR+SHR+FFTKSDLFFVCMLQP SF +QKQ S
Sbjct: 224 EGEDICEAAIREVKEETGVDAEFVEVLAFRESHRSFFTKSDLFFVCMLQPRSFAVQKQNS 283

Query: 261 EIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYS 320
           EIEAAKWMPIDEYADQPFVK NSGFDYVAKVCLAK S+NYTGLSA PTYS+SGK TYLYS
Sbjct: 284 EIEAAKWMPIDEYADQPFVKKNSGFDYVAKVCLAK-SNNYTGLSAIPTYSSSGKKTYLYS 343

Query: 321 KLE 324
           KL+
Sbjct: 344 KLD 343

BLAST of Spg006161 vs. ExPASy TrEMBL
Match: A0A6J1EL41 (nudix hydrolase 7-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435315 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.8e-143
Identity = 262/303 (86.47%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 21  LFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGV 80
           L  I+  AA  SYL+NK+ + SRGMSSSSTSLAI+E E P DNIEQLELL GVYD+YEGV
Sbjct: 44  LLGIITLAA--SYLSNKTPSLSRGMSSSSTSLAISENETPLDNIEQLELLPGVYDNYEGV 103

Query: 81  IVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPN 140
           IVEMKDPMDP EFG LL+ASLSQWKQLGKRGIWIKLPI+LANL+EVVVKEGFVFHHAEP+
Sbjct: 104 IVEMKDPMDPNEFGSLLRASLSQWKQLGKRGIWIKLPIKLANLIEVVVKEGFVFHHAEPD 163

Query: 141 YLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVN 200
           YLMLVRWISETTN++P NASHRVGIGAFVMNSNREVLVVQEISGKFKG+GVWKLPTGVVN
Sbjct: 164 YLMLVRWISETTNSIPANASHRVGIGAFVMNSNREVLVVQEISGKFKGTGVWKLPTGVVN 223

Query: 201 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVS 260
           EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHR+FFTKSDLFFVCMLQP SF +QKQ S
Sbjct: 224 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRSFFTKSDLFFVCMLQPRSFAVQKQNS 283

Query: 261 EIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYS 320
           EIEAAKWMPIDEYADQ FVK NSGFDYVAKVCLAKA++NYTGLSA PTYS+SGK TYLYS
Sbjct: 284 EIEAAKWMPIDEYADQTFVKKNSGFDYVAKVCLAKANNNYTGLSAIPTYSSSGKKTYLYS 343

Query: 321 KLE 324
           KL+
Sbjct: 344 KLD 344

BLAST of Spg006161 vs. ExPASy TrEMBL
Match: A0A6J1C541 (nudix hydrolase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008005 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.8e-143
Identity = 255/295 (86.44%), Postives = 280/295 (94.92%), Query Frame = 0

Query: 29  ATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPM 88
           A++SYLTNK L+ S+GMSSSSTSLA AE+EI QDNIEQLELLSGV+DHY GVIVEMKDP+
Sbjct: 50  ASYSYLTNKRLSLSQGMSSSSTSLASAEEEIRQDNIEQLELLSGVHDHYGGVIVEMKDPI 109

Query: 89  DPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 148
           DPKEFG LL+ASLSQW+QLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI
Sbjct: 110 DPKEFGSLLRASLSQWRQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 169

Query: 149 SETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEA 208
           SETTN+LP NASHRVGIGAFVMN+NREVLVVQEI GKFKGSGVWKLPTGVVNEGEDICEA
Sbjct: 170 SETTNSLPHNASHRVGIGAFVMNTNREVLVVQEIGGKFKGSGVWKLPTGVVNEGEDICEA 229

Query: 209 AIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWM 268
           AIREVKEET +DAEFVEVLAFRQSH +FFTKSDLFFVCML+P S+DIQKQ SEIEAAKWM
Sbjct: 230 AIREVKEETDIDAEFVEVLAFRQSHNSFFTKSDLFFVCMLRPSSYDIQKQASEIEAAKWM 289

Query: 269 PIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYSKLE 324
           PIDEYADQPFV+ NS FDYVAKVC+AKA+++YTGLSA PT+S+SGK++YLYSKL+
Sbjct: 290 PIDEYADQPFVRANSAFDYVAKVCVAKANNDYTGLSANPTFSSSGKLSYLYSKLK 344

BLAST of Spg006161 vs. ExPASy TrEMBL
Match: A0A6J1C770 (nudix hydrolase 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008005 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.8e-143
Identity = 255/295 (86.44%), Postives = 280/295 (94.92%), Query Frame = 0

Query: 29  ATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPM 88
           A++SYLTNK L+ S+GMSSSSTSLA AE+EI QDNIEQLELLSGV+DHY GVIVEMKDP+
Sbjct: 30  ASYSYLTNKRLSLSQGMSSSSTSLASAEEEIRQDNIEQLELLSGVHDHYGGVIVEMKDPI 89

Query: 89  DPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 148
           DPKEFG LL+ASLSQW+QLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI
Sbjct: 90  DPKEFGSLLRASLSQWRQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWI 149

Query: 149 SETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEA 208
           SETTN+LP NASHRVGIGAFVMN+NREVLVVQEI GKFKGSGVWKLPTGVVNEGEDICEA
Sbjct: 150 SETTNSLPHNASHRVGIGAFVMNTNREVLVVQEIGGKFKGSGVWKLPTGVVNEGEDICEA 209

Query: 209 AIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWM 268
           AIREVKEET +DAEFVEVLAFRQSH +FFTKSDLFFVCML+P S+DIQKQ SEIEAAKWM
Sbjct: 210 AIREVKEETDIDAEFVEVLAFRQSHNSFFTKSDLFFVCMLRPSSYDIQKQASEIEAAKWM 269

Query: 269 PIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYSKLE 324
           PIDEYADQPFV+ NS FDYVAKVC+AKA+++YTGLSA PT+S+SGK++YLYSKL+
Sbjct: 270 PIDEYADQPFVRANSAFDYVAKVCVAKANNDYTGLSANPTFSSSGKLSYLYSKLK 324

BLAST of Spg006161 vs. ExPASy TrEMBL
Match: A0A1S3CHH2 (nudix hydrolase 7-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500506 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.5e-141
Identity = 262/303 (86.47%), Postives = 274/303 (90.43%), Query Frame = 0

Query: 21  LFVILAAAATFSYLTNKSLTPSRGMSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGV 80
           LF      AT SY+ NKS T SRGMSSSS +L   EK  P  NI+QLELLSGVYD Y+GV
Sbjct: 44  LFSFRTLKATASYVGNKSPTLSRGMSSSSATL---EKVTPPANIQQLELLSGVYDRYDGV 103

Query: 81  IVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPN 140
           IVEMKDPMDP EFG LL+ASLSQWKQ GKRGIWIKLPIELANL+EVVVKEGF FHHAEPN
Sbjct: 104 IVEMKDPMDPNEFGSLLRASLSQWKQQGKRGIWIKLPIELANLIEVVVKEGFDFHHAEPN 163

Query: 141 YLMLVRWISETTNNLPPNASHRVGIGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVN 200
           YLMLVRWISETTNNLP NASHRVGIGAFVMNSNREVLVVQEISGKFKG+GVWKLPTGVVN
Sbjct: 164 YLMLVRWISETTNNLPANASHRVGIGAFVMNSNREVLVVQEISGKFKGTGVWKLPTGVVN 223

Query: 201 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVS 260
           EGEDICEAAIREVKEETGVDAEFVEVLAFRQSH AFFTKSDLFFVCML+P SFDIQKQ S
Sbjct: 224 EGEDICEAAIREVKEETGVDAEFVEVLAFRQSHSAFFTKSDLFFVCMLRPRSFDIQKQAS 283

Query: 261 EIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLYS 320
           EIEAAKWMPIDEYADQPFVK NSGFDYVAKVCLAKA+++YTGLSA PTYSASGKMT+LYS
Sbjct: 284 EIEAAKWMPIDEYADQPFVKANSGFDYVAKVCLAKANNSYTGLSAMPTYSASGKMTFLYS 343

Query: 321 KLE 324
           KLE
Sbjct: 344 KLE 343

BLAST of Spg006161 vs. TAIR 10
Match: AT5G47650.1 (nudix hydrolase homolog 2 )

HSP 1 Score: 305.1 bits (780), Expect = 6.9e-83
Identity = 151/260 (58.08%), Postives = 187/260 (71.92%), Query Frame = 0

Query: 45  MSSSSTSLAIAEKEIPQDNIEQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQW 104
           MS+SS+S        P    +   LL  V D Y GV+ EM  PMDP  F  LL++SLS W
Sbjct: 1   MSASSSSTN------PMSREDATTLLPSVQDKYGGVMTEMTHPMDPSLFSTLLRSSLSTW 60

Query: 105 KQLGKRGIWIKLPIELANLVEVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVG 164
              GK+G+WIKLP +L  L E  VKEGF FHHAE +YLMLV WI +  + LP NASHRVG
Sbjct: 61  TLQGKKGVWIKLPKQLIGLAETAVKEGFWFHHAEKDYLMLVYWIPKEDDTLPANASHRVG 120

Query: 165 IGAFVMNSNREVLVVQEISGKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFV 224
           IGAFV+N N+EVLVVQE +G+F+G G+WK PTGVVNEGEDI + ++REVKEETGVD EF 
Sbjct: 121 IGAFVINHNKEVLVVQEKTGRFQGQGIWKFPTGVVNEGEDIHDGSVREVKEETGVDTEFD 180

Query: 225 EVLAFRQSHRAFFTKSDLFFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSG 284
           ++LAFRQ+H+AFF KSDLFFVCML+P S +I  Q SEIEAA+WMP +EY +QPFV+N   
Sbjct: 181 QILAFRQTHKAFFGKSDLFFVCMLKPLSLEINAQESEIEAAQWMPWEEYINQPFVQNYEL 240

Query: 285 FDYVAKVCLAKASDNYTGLS 305
             Y+  +C AK + +Y G +
Sbjct: 241 LRYMTDICSAKTNGDYEGFT 254

BLAST of Spg006161 vs. TAIR 10
Match: AT4G12720.1 (MutT/nudix family protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 150/257 (58.37%), Postives = 188/257 (73.15%), Query Frame = 0

Query: 65  EQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLV 124
           +Q+ LL G  D+Y+GV V M +PMD + F   L+ASLS W++ GK+GIWIKLP+ LANLV
Sbjct: 6   QQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLV 65

Query: 125 EVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSN-REVLVVQEIS 184
           E  V EGF +HHAEP YLMLV WISET + +P NASH VG GA V+N N +EVLVVQE S
Sbjct: 66  EAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERS 125

Query: 185 GKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-TKSDL 244
           G FK   VWKLPTGV+NEGEDI     REV+EETG+ A+FVEVLAFRQSH+A    K+D+
Sbjct: 126 GFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDM 185

Query: 245 FFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTG 304
           FF+C+L P S+DI +Q SEI  AKWMPI EY DQP+ K N  F ++A +C  K  + Y G
Sbjct: 186 FFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLG 245

Query: 305 LSAKPTYSASGKMTYLY 320
            +  PT ++SGK +++Y
Sbjct: 246 FAIVPTTTSSGKESFIY 262

BLAST of Spg006161 vs. TAIR 10
Match: AT4G12720.2 (MutT/nudix family protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 150/257 (58.37%), Postives = 188/257 (73.15%), Query Frame = 0

Query: 65  EQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLV 124
           +Q+ LL G  D+Y+GV V M +PMD + F   L+ASLS W++ GK+GIWIKLP+ LANLV
Sbjct: 6   QQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLV 65

Query: 125 EVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSN-REVLVVQEIS 184
           E  V EGF +HHAEP YLMLV WISET + +P NASH VG GA V+N N +EVLVVQE S
Sbjct: 66  EAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERS 125

Query: 185 GKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-TKSDL 244
           G FK   VWKLPTGV+NEGEDI     REV+EETG+ A+FVEVLAFRQSH+A    K+D+
Sbjct: 126 GFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDM 185

Query: 245 FFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTG 304
           FF+C+L P S+DI +Q SEI  AKWMPI EY DQP+ K N  F ++A +C  K  + Y G
Sbjct: 186 FFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLG 245

Query: 305 LSAKPTYSASGKMTYLY 320
            +  PT ++SGK +++Y
Sbjct: 246 FAIVPTTTSSGKESFIY 262

BLAST of Spg006161 vs. TAIR 10
Match: AT4G12720.3 (MutT/nudix family protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 150/257 (58.37%), Postives = 188/257 (73.15%), Query Frame = 0

Query: 65  EQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLV 124
           +Q+ LL G  D+Y+GV V M +PMD + F   L+ASLS W++ GK+GIWIKLP+ LANLV
Sbjct: 6   QQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLV 65

Query: 125 EVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSN-REVLVVQEIS 184
           E  V EGF +HHAEP YLMLV WISET + +P NASH VG GA V+N N +EVLVVQE S
Sbjct: 66  EAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERS 125

Query: 185 GKFKGSGVWKLPTGVVNEGEDICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-TKSDL 244
           G FK   VWKLPTGV+NEGEDI     REV+EETG+ A+FVEVLAFRQSH+A    K+D+
Sbjct: 126 GFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDM 185

Query: 245 FFVCMLQPCSFDIQKQVSEIEAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTG 304
           FF+C+L P S+DI +Q SEI  AKWMPI EY DQP+ K N  F ++A +C  K  + Y G
Sbjct: 186 FFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLG 245

Query: 305 LSAKPTYSASGKMTYLY 320
            +  PT ++SGK +++Y
Sbjct: 246 FAIVPTTTSSGKESFIY 262

BLAST of Spg006161 vs. TAIR 10
Match: AT4G12720.4 (MutT/nudix family protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-76
Identity = 150/297 (50.51%), Postives = 188/297 (63.30%), Query Frame = 0

Query: 65  EQLELLSGVYDHYEGVIVEMKDPMDPKEFGFLLKASLSQWKQLGKRGIWIKLPIELANLV 124
           +Q+ LL G  D+Y+GV V M +PMD + F   L+ASLS W++ GK+GIWIKLP+ LANLV
Sbjct: 6   QQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLV 65

Query: 125 EVVVKEGFVFHHAEPNYLMLVRWISETTNNLPPNASHRVGIGAFVMNSN-REVLVVQEIS 184
           E  V EGF +HHAEP YLMLV WISET + +P NASH VG GA V+N N +EVLVVQE S
Sbjct: 66  EAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERS 125

Query: 185 GKFKGSGVWKLPTGVVNE----------------------------------------GE 244
           G FK   VWKLPTGV+NE                                        GE
Sbjct: 126 GFFKDKNVWKLPTGVINEELVVLRVVGELHKKVVLTSYRSIKCLLIICNDTKNETKRMGE 185

Query: 245 DICEAAIREVKEETGVDAEFVEVLAFRQSHRAFF-TKSDLFFVCMLQPCSFDIQKQVSEI 304
           DI     REV+EETG+ A+FVEVLAFRQSH+A    K+D+FF+C+L P S+DI +Q SEI
Sbjct: 186 DIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDMFFLCVLSPRSYDITEQKSEI 245

Query: 305 EAAKWMPIDEYADQPFVKNNSGFDYVAKVCLAKASDNYTGLSAKPTYSASGKMTYLY 320
             AKWMPI EY DQP+ K N  F ++A +C  K  + Y G +  PT ++SGK +++Y
Sbjct: 246 LQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLGFAIVPTTTSSGKESFIY 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022790.11.9e-14386.80Nudix hydrolase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023529751.13.3e-14386.47nudix hydrolase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022136267.11.6e-14286.44nudix hydrolase 2-like isoform X1 [Momordica charantia][more]
XP_022989436.11.6e-14286.80nudix hydrolase 7-like isoform X1 [Cucurbita maxima] >XP_022989437.1 nudix hydro... [more]
XP_022136268.11.6e-14286.44nudix hydrolase 2-like isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q94B749.7e-8258.08Nudix hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=NUDT2 PE=1 SV=1[more]
Q9SU143.7e-8158.37Nudix hydrolase 7 OS=Arabidopsis thaliana OX=3702 GN=NUDT7 PE=1 SV=1[more]
Q6NPD79.7e-7453.33Nudix hydrolase 10 OS=Arabidopsis thaliana OX=3702 GN=NUDT10 PE=1 SV=1[more]
Q9SJC47.0e-7253.82Nudix hydrolase 6 OS=Arabidopsis thaliana OX=3702 GN=NUDT6 PE=1 SV=1[more]
Q9SJC66.1e-6852.07Nudix hydrolase 5 OS=Arabidopsis thaliana OX=3702 GN=NUDT5 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1JK237.8e-14386.80nudix hydrolase 7-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486492 PE... [more]
A0A6J1EL417.8e-14386.47nudix hydrolase 7-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435315 ... [more]
A0A6J1C5417.8e-14386.44nudix hydrolase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008005... [more]
A0A6J1C7707.8e-14386.44nudix hydrolase 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008005... [more]
A0A1S3CHH22.5e-14186.47nudix hydrolase 7-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500506 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G47650.16.9e-8358.08nudix hydrolase homolog 2 [more]
AT4G12720.12.6e-8258.37MutT/nudix family protein [more]
AT4G12720.22.6e-8258.37MutT/nudix family protein [more]
AT4G12720.32.6e-8258.37MutT/nudix family protein [more]
AT4G12720.42.1e-7650.51MutT/nudix family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003293Nudix hydrolase 6-likePRINTSPR01356GFGPROTEINcoord: 158..176
score: 26.32
coord: 142..158
score: 25.49
coord: 246..270
score: 34.67
coord: 120..138
score: 43.86
IPR003293Nudix hydrolase 6-likePANTHERPTHR13994NUDIX HYDROLASE RELATEDcoord: 31..320
IPR040618Pre-nudix hydrolase domainPFAMPF18290Nudix_hydrocoord: 70..148
e-value: 1.4E-30
score: 105.1
NoneNo IPR availableGENE3D3.40.630.30coord: 67..152
e-value: 2.8E-30
score: 106.0
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 162..293
e-value: 1.3E-34
score: 121.0
NoneNo IPR availablePANTHERPTHR13994:SF29NUDIX HYDROLASE 2coord: 31..320
NoneNo IPR availableCDDcd04670Nudix_Hydrolase_12coord: 161..279
e-value: 2.952E-64
score: 197.143
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 161..281
e-value: 3.3E-22
score: 78.9
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 160..292
score: 17.858562
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 197..218
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 127..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006161.1Spg006161.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0047631 ADP-ribose diphosphatase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0035529 NADH pyrophosphatase activity
molecular_function GO:0016787 hydrolase activity