Spg005683 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg005683 vs. NCBI nr
Match: PWA36168.1 (hypothetical protein CTI12_AA602590 [Artemisia annua])

HSP 1 Score: 188.0 bits (476), Expect = 4.4e-43
Identity = 167/667 (25.04%), Postives = 287/667 (43.03%), Query Frame = 0

Query: 435  AKVSHLTRIASDHRSLLAEWSIEPLNPSFVIPRRPRRFEEGWCKYGECREIVATVWN--- 494
            A   +L RIASDH  ++       L+P      R  RFE  W +      +V   W    
Sbjct: 480  ASFENLPRIASDHSPIICR-----LSPMVKKKNRMFRFESMWLRDKSIHGVVRDGWAYGL 539

Query: 495  SQGC-HDISDFNTKIMDCLMSLNHWSRRKYGGSIRGAIAKTERDIQHLSKKDDQTSRTAL 554
            + G  HD       + +C   L+ W++R + G ++ +I   +R +Q L  + D ++R   
Sbjct: 540  AAGMQHDPCGI---VSECANRLSDWNKRSF-GHVQRSIKSKQRSLQTLQSRFDGSTRAEQ 599

Query: 555  REKEKTLESLLEDDEIYWKQRSREEWLLWGDRNTKWFHMRANTRRKRNRIRGIMNDLGIW 614
            +   + ++ LL  +E+ WKQRSR EWL  GD+NT++FH RA+ R++RN I  +    G W
Sbjct: 600  QALREQIKELLTREELMWKQRSRIEWLREGDKNTRFFHTRASNRQRRNSILRLKGPDGRW 659

Query: 615  TEEDNGMEFIVNNYFAKLFQSSEPQ-MDSIAHILE-----------SIPTSISEAQ---- 674
             EE N +  +V++YF+ LF SS PQ  +S+   ++             P + SE +    
Sbjct: 660  VEEHNEVCKLVSSYFSDLFSSSSPQGCESVVRDIDRRLTENERQALERPVTSSEVRDLLN 719

Query: 675  ------NNDLEDALAILATPTK------SNMGEDEILWNLDSKGRFSVKGAYRLGCQMNQ 734
                  N++L  +L      +K      S    D + W+ +  GRFS K AY L  + ++
Sbjct: 720  TEGDGWNHELMYSLFPHNIASKIGCCFISKSRNDILYWHNNPGGRFSCKSAYLLALEADE 779

Query: 735  RFQASSANYKDQEAMWKDFWKLKLPPKIKICGWRIYNDILPTLSNLNNRGMDVWPVCFLC 794
                ++         W+  WK ++P K+K+  WR +N+ +PT+ NL +RG++    C  C
Sbjct: 780  DMVRTTTISNSLIDFWRVVWKARVPSKVKLFMWRAWNNYVPTIDNLKSRGLNPTSSCTHC 839

Query: 795  REKEETTSHLFWHCKMTKGLWAKYLPLDNLSCLFDRED--------RRISETLDGLWQRG 854
             +  E   H+ + C + K +W +     N  C +D +         + I E     W+  
Sbjct: 840  GQTSENLVHVLFKCSVAKDVWNRC----NFGCFYDTQGAITFQDFCQVILEKFLAEWE-- 899

Query: 855  GNTSTNILHIKCSLIICWRIWSIRNLISHNNQRLNQETIRDILQQQISASIHELIGDEEP 914
                         ++I W +W+ RN   H  Q   +E   +++ + + +  H+       
Sbjct: 900  -----------TFMMILWGLWTRRNRHFH-GQLNGREGNVEVIAKSVLSDYHK------- 959

Query: 915  YQMQWLEGQTERLAPSGRGGVLLGDPTVRRKWSPISDGCW--------KLSYDASWRSDR 974
                    Q E +  SG  GV             I  G W        K++ DA+W+ + 
Sbjct: 960  ------ANQRENI--SGNSGV-----------HNIHAGMWLRPDVEHIKINCDAAWQKES 1019

Query: 975  ECGSVGWVLRDWSRTLLAAGFKCINSASDISWLEALSIVEGLQAIPSVTGGVRLLVENDS 1034
                +G+V R++   +L +G +    AS     EA ++   +  +    G   ++ E +S
Sbjct: 1020 GKAGLGFVARNYKGEVLFSGGRSECYASSPLEAEAKAVHWAMTRV-ICKGYSHVIFETNS 1079

Query: 1035 LQVVNLINGEDVDETELNFFIKEAQRMCSIKKVDSITHIPRAHNYLAHSLAQKAYEEDGP 1054
            L +V  +  + V   ++     E         V + + + R  N LAHS+A  A      
Sbjct: 1080 LCLVKALQNK-VTLLQIASLFSEILSKLMAFNVCNWSFVKREGNRLAHSIASLAL----- 1085

BLAST of Spg005683 vs. NCBI nr
Match: GAU41525.1 (hypothetical protein TSUD_140560 [Trifolium subterraneum])

HSP 1 Score: 188.0 bits (476), Expect = 4.4e-43
Identity = 176/735 (23.95%), Postives = 294/735 (40.00%), Query Frame = 0

Query: 22  EERASVFKLKEEEIDRSEKKLENALVCKILSQKQINPEMFKSKMSHIWNQEQTI-ISMVG 81
           E+      ++ EEI   E+  +  LV K+ ++   N   FK  ++  W  + +I +  + 
Sbjct: 12  EDEEDCITVEAEEICEEEETFKRTLVGKLWTENPYNVRAFKQTIAQAWRLKNSIEVQDLE 71

Query: 82  FNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKLPF 141
            NL+L +F   K    +  NGPW FD+ LL+     G     D++   V+FW+  + LPF
Sbjct: 72  ENLFLFRFTTKKDADTVLRNGPWSFDRNLLILHRVSGEEQPSDLDMHNVNFWVRVYDLPF 131

Query: 142 ACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDATKPLKRGIFLSSEDSTE 201
              S  +A ++G+I+G   ++D ++      G  LR+K  +D  KPLKRG  +  +D  +
Sbjct: 132 KLRSEAMAKKLGNIMGNFEEVDPKDA--NRTGRFLRLKASLDLRKPLKRGTKIKFQD--K 191

Query: 202 DRWIPITYEKLPDFCYGCGLLGHTLKECEG------SNHDGSPVEELPYGAWLREPVLLK 261
           + W+   YE+LP+FC+ CG +GH +KECE       +N+     +   YG WLR   L +
Sbjct: 192 NMWVDFKYERLPNFCFACGKIGHQMKECEDVEDVDENNYSDIAEKSQAYGPWLRASPLPR 251

Query: 262 AREGGWRGGHHHDEEAYGAGGNERRQGGTEEATIRDQPSASGPPTMNKPPANIPTGAGPE 321
             E   +              + + +GG  E     +      P +      +PT A   
Sbjct: 252 IFEEPRKDASSGSCSKNLFPSSSQSKGGQSEGKKDKEQEVDQQPVVGIEKQLVPTKATNN 311

Query: 322 TD----------------------------------TMANNSDMERVEKVTELKKVPVKE 381
            D                                      +S   + +   +L K   K 
Sbjct: 312 LDVEDVAESLGAVAISTSFVVGKTTKSQGGSKGRKWVRNKSSKPVKAQTARKLAKELGKR 371

Query: 382 GEIKVSLKD---------------NFISKGKVNSPNMDEDSGNNG----------PIGKE 441
             + V++ +                F+ + ++  P +D      G           +G+E
Sbjct: 372 NLVDVAISEVRALSRLIRLENPQVVFLMETRLKVPEIDRLKFKLGFSSGLAIDCKGVGRE 431

Query: 442 KESYCSIMEVDLEDNGSK------VEATSTEVKSTQDNDV-GVFLDPKGKGVL------- 501
           +    ++   D  D   K      +     +V++ +  D+ G++ + K  G++       
Sbjct: 432 RAGGLALFWKDHMDITIKSYSLNHIHGQCVDVETNEPWDLTGIYAEEKKGGLVRTQGQLE 491

Query: 502 ---------------------------------------CDNSSNFSKGKAKAKVSHLTR 561
                                                    +S  F    +   V HL R
Sbjct: 492 IGRQAILESRLNDLGFEGYPLTWSNGRNSDDNKQCRQDRAMSSDEFINRFSPIHVLHLPR 551

Query: 562 IASDHRSLLAEWSIEPLNPSFVIPRRPR--RFEEGWCKYGECREIVATVWNSQGCHDISD 621
             SDH  L+               RR R  RFEE W     C  ++   W SQ C   SD
Sbjct: 552 YGSDHVVLVITLEAPTHRDQRRRRRRKRLFRFEESWTSNARCEPLIQACW-SQPCLSFSD 611

Query: 622 FNTKIMDCLMSLNHWSRRKYGGSIRGAIAKTERDIQHLSKKDD-QTSRTALREKEKTLES 635
              ++ D    L   S     GSI   I + E+ IQ+    D+ +TS    +  E TLE 
Sbjct: 612 KLGRLRDMGNELGDHS----VGSIHKEIVRIEKLIQNHDMWDESETSIQRFKALEHTLEE 671

BLAST of Spg005683 vs. NCBI nr
Match: GAU41525.1 (hypothetical protein TSUD_140560 [Trifolium subterraneum])

HSP 1 Score: 90.1 bits (222), Expect = 1.3e-13
Identity = 42/122 (34.43%), Postives = 64/122 (52.46%), Query Frame = 0

Query: 659  EDALAILATPTKSNMGEDEILWNLDSKGRFSVKGAYRLGCQMNQRFQASSANYKDQEAMW 718
            ++AL I++TP   N+  D+I+W+ +  G +SV+ AY L C           +    +++W
Sbjct: 1319 DEALKIVSTPLSLNLPADKIIWHWERDGVYSVRPAYHLLCDARD-INLPGPSSSGDDSLW 1378

Query: 719  KDFWKLKLPPKIKICGWRIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKM 778
            K  WK  +P K+K    R+  +ILPT  NL  +G+ +   C LC    E   HLF HC M
Sbjct: 1379 KKIWKAPIPNKVKNFMSRLAKNILPTRDNLKKKGISLDTSCPLCHNDSENAHHLFMHCNM 1438

Query: 779  TK 781
             K
Sbjct: 1439 LK 1439

HSP 2 Score: 185.7 bits (470), Expect = 2.2e-42
Identity = 189/699 (27.04%), Postives = 310/699 (44.35%), Query Frame = 0

Query: 20  TEEERASVFKLKEEEIDRSEKKLENALVCKILSQKQINPEMFKSKMSHIWN-QEQTIISM 79
           TEEE   + +  E E D    K   +L  K+ +    N   FK  +   W  + Q  I  
Sbjct: 11  TEEEIGVIAEDLETEEDGYFTK---SLAGKLWTTSSYNIRAFKQSIVEFWKLKNQVEIQD 70

Query: 80  VGFNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKL 139
           +G NLYL +F   +    +  NGPW FD+ +L+ K   G     +I+   +SFW   + L
Sbjct: 71  LGKNLYLFRFSTKRDLEHVLKNGPWSFDRNILVLKRISGIEQPSEIDMSSMSFWARVYDL 130

Query: 140 PFACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDATKPLKRGIFLSSEDS 199
           P    S  +A ++G ++G+  +ID +E  +   G  L++K+ ID  KP+KRG  +  +  
Sbjct: 131 PLKLRSESIAKKLGDLMGKFEEIDNKE--NHRMGCFLKIKVTIDLKKPMKRGTVVKFQG- 190

Query: 200 TEDRWIPITYEKLPDFCYGCGLLGHTLKECEGSN-HDGSPVE-----ELPYGAWLREPVL 259
            +D  +   YE+LP FC+ CG +GH ++ECE +   DG   E     ELP+G WLR   L
Sbjct: 191 -KDLRVFFKYERLPIFCFVCGRIGHQIRECEETEAQDGEGFEEIEEKELPFGPWLRASPL 250

Query: 260 LKA-----REGG--------WRGGHHHDEEAYG--AGGNERRQGGTEEATIRDQPSASGP 319
            +A     +E G        + G      ++ G   G  E  Q  T+   I  Q   +  
Sbjct: 251 PRANYVAKKESGSSSCSKNLFSGSSSSRADSVGDKMGEVEVDQAITKMKAIEIQLPNTNA 310

Query: 320 PTMNKPPANIP-------------TGAGPETDTMANNSDMERVEKVTELKKVPVKEGEIK 379
            T +     I              + A P+          E  +    LK      G+ +
Sbjct: 311 VTNSTAKTFIEIEKVAESLGSVDISLASPKKGGWGTLGQFEPCQGSKTLKIPTFCHGKDR 370

Query: 380 ---VSLKDNFISKGKVNSPNMDEDSGN-NGPIGKEKESYCSIMEVDLEDNGSKVEATSTE 439
              ++L  N  +   ++S +++  +G  +  +  E  S   I     E N  K  A   E
Sbjct: 371 AGGIALWWNDTTNITISSYSLNHINGKIDDDLNGESWSITRIYGFPEETNKRKTWALINE 430

Query: 440 VK-STQDN-----DVGVFLDPKGKG-------------VLCDNSSNFSKGKAKAKVSHLT 499
           +  ST +N     D+   L    K               LC  SS+F+      KV+HL 
Sbjct: 431 ISASTAENWICFGDLNDTLSASEKAGEKARRTLSVVLIGLCMASSSFTNRFGPIKVTHLP 490

Query: 500 RIASDHRSLLAEWSIEPLNPSFVIPRRPR--RFEEGWCKYGECREIVATVW-NSQGCHDI 559
           R  SDH ++     +   + +    ++P   RFEE W K   C  ++   W N++G    
Sbjct: 491 RFGSDHSAIQI---LLEAHDNVGRNKKPHIFRFEECWSKDRSCESLIKKYWSNTRG---- 550

Query: 560 SDFNTKIMDCLMSLNHWSRRKYGGSIRGAIAKTERDIQHLSKKDDQTSR-TALREKEKTL 619
            D  TK++  L SL+   +      ++  I KTE  ++     D         RE E+  
Sbjct: 551 -DCETKLV-ALQSLDIDFKHYRSTEVKKEILKTEEALKDECLWDGSKEEIQRYREMERKH 610

Query: 620 ESLLEDDEIYWKQRSREEWLLWGDRNTKWFHMRANTRRKRNRIRGIMNDLGIWTEEDNGM 657
             LL+ +E+ W+QRSR  WL  GD+NTK+FH +A+ R+K N I+ + ++ GIW    + +
Sbjct: 611 SELLQQEELLWRQRSRAVWLKDGDKNTKFFHGKASQRKKTNAIKKLKDEDGIWWHGQDKV 670

BLAST of Spg005683 vs. NCBI nr
Match: TXG58188.1 (hypothetical protein EZV62_016017 [Acer yangbiense])

HSP 1 Score: 184.1 bits (466), Expect = 6.4e-42
Identity = 173/633 (27.33%), Postives = 266/633 (42.02%), Query Frame = 0

Query: 56  INPEMFKSKMSHIWN-QEQTIISMVGFNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKE 115
           +N E FK  +  IWN   Q  + MV  N+++  F N + ++ I    PW F  +L+  ++
Sbjct: 1   MNREAFKGLIEQIWNPYGQVEVEMVRDNIFMFYFLNQEDRNRIWQRSPWHFGNSLIALEK 60

Query: 116 PKGGNYGDDIEFRYVSFWIHFHKLPFACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGT 175
           P G      + F    FWI  H +P  C +R  A  +   +G V +I LE    +C G  
Sbjct: 61  PDGPGNISMLGFNKADFWIQIHDIPILCMNRRTAKWMAEQIGVVVEIPLESR--ECWGKY 120

Query: 176 LRVKIQIDATKPLKRGIFLSSEDSTEDRWIPITYEKLPDFCYGCGLLGHTLKECEGSNHD 235
           +RVK+ ID +KPLKR + L    S E   + + YEKLP+FCY CG +G  + EC  +   
Sbjct: 121 MRVKVCIDISKPLKRWLRLKLSRSDEITTVSLKYEKLPEFCYACGRIGRGIMECWDNEAR 180

Query: 236 GSPVEELP--YGAWLREPVLLKAREGGWRGGHHHDEEAYGAGGN-------ERRQGGTEE 295
              +E  P  YG+WL+   L K++    +       + YG+  +        R + G   
Sbjct: 181 KIALEGNPTKYGSWLKAATLEKSKS---KSKSKSQSQGYGSSSDRNRSMEGSREREGVGS 240

Query: 296 ATIRDQPSASGPPTMNKPPANI----------PTGAGP-ETDTMA--------------- 355
           A++R    AS         A I           T  GP  T+ M                
Sbjct: 241 ASLRQGSLASQEKVPAASAAAIGERLKGALAEATDVGPAHTEDMCLDGPNLGTLKPSQEM 300

Query: 356 ---NNSDMERVEK---VTELKKVPVKEGEIKVSLKDNFISKGK-VNSPNMD-EDSGNNGP 415
              NNSD   VE       L     +  E+K  ++D   S  K ++SP    + S    P
Sbjct: 301 DPINNSDSINVEPKFVAPNLTTTITQTFEVKPKIQDQDTSHFKPISSPFPQLQQSLIPTP 360

Query: 416 IGKEKESYCSIMEVDLEDN---------GSKVEATSTEVKSTQDNDVGVFLDPK--GKGV 475
           +   K+      + +  D+          S ++      KS + +       PK    G+
Sbjct: 361 LQTLKKKSSRKWKRNARDSKHQLKPGLPSSPLQRMLDIRKSPEKSKSVKVSSPKVSKTGL 420

Query: 476 LCDNSSNFSKGKAKAKVSHL-------TRIASDHRSLLAEWSIEPLNPSFVIPRRPRR-F 535
           L  N+ +  K +   +  H        TR+ S+  +   +   E     F  P++ +  F
Sbjct: 421 LSSNAKSPQKSQPPNQKPHSEQTEVVNTRLGSEGNACKRKMVFENSQEDFRAPKKGKNDF 480

Query: 536 EE------GWCKYGECREIVATVWNSQG-CHDISDFNTKIMDCLMSLNHWSRRKYGGSIR 595
            +       W K  E   I+  +W   G    I D   K+  C  +L  WS+ ++G   R
Sbjct: 481 TDLEISISFWLKEKEIGRIIKDIWQGSGLISSIGDLKLKLDKCASNLTVWSKTRFGNIQR 540

Query: 596 GAIAKTERDIQHLSKKDDQTS-RTALREKEKTLESLLEDDEIYWKQRSREEWLLWGDRNT 618
               KT R+I+HL            +R  E+ +E L + +EIYWKQRSR EWL  GDRN+
Sbjct: 541 QFREKT-REIEHLYNLCGSPGIMILIRSLEREVEGLYDCEEIYWKQRSRAEWLKVGDRNS 600

BLAST of Spg005683 vs. NCBI nr
Match: TXG53848.1 (hypothetical protein EZV62_019104 [Acer yangbiense])

HSP 1 Score: 181.8 bits (460), Expect = 3.2e-41
Identity = 174/663 (26.24%), Postives = 295/663 (44.49%), Query Frame = 0

Query: 6   AEETLVKQLSGLKVTEEERASVFKLKEEEIDRSEKKLENALVCKILSQKQINPEMFKSKM 65
           AE  +VK    L + +E+ A + ++ EE+    E +++  LV KILS K++N E F   +
Sbjct: 2   AESEIVKLYENLSLADEDGA-IHEMPEEDQREGEVEVDLCLVGKILSGKKVNREAFIHLI 61

Query: 66  SHIWNQ-EQTIISMVGFNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDI 125
             +W+   +  I  VG N+++  F N + ++ I   GPW+FDK+L++ ++ +G      +
Sbjct: 62  EQLWSPFGRVAIESVGVNIFMFHFNNQEERNRIWQRGPWYFDKSLIVLEKSEGMGNISHL 121

Query: 126 EFRYVSFWIHFHKLPFACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDAT 185
            F  V  WI  H +P  C +R  A  +   +G+V  ID+  E   C G  ++VK+QID +
Sbjct: 122 RFNKVELWIQIHDVPIICMNRITAKWMAEQIGRV--IDIPTESKDCWGKFMKVKVQIDIS 181

Query: 186 KPLKRGIFLSSEDSTEDRWIPITYEKLPDFCYGCGLLGHTLKECEGSNHDGSPVEELPYG 245
           KPLK             RW+ +  +K  +   G                     +   +G
Sbjct: 182 KPLK-------------RWLRLKLDKSNNIVMG---------------------DSTKFG 241

Query: 246 AWLREPVLLKAREGGWRGGHHHDEEAYGAGGNERRQGGTEEATIRDQPSASGPPTMNKPP 305
           +W+R   L + +    R  H  D       GN + Q   EE    ++   SG  +     
Sbjct: 242 SWMRASALDRQK---LRIDHQID-------GNAKIQSRLEERHEGNEVRLSGHGSSQIHG 301

Query: 306 ANIPTGAGPETDTMANNSDMERVEKVTELKKVPVKEGEIKVSLKDNFISKGKVNSPNMDE 365
            ++    GP  +            K+   K V V         K+ F   G + S +++ 
Sbjct: 302 LSL---EGPSEEA-----------KLRSKKSVEVG--------KEAF-GPGSMVSNSINS 361

Query: 366 DSGNNGPIGKEKESYCSIMEVDLEDNGSKVEATSTE----VKSTQDNDVGVFLDPKGKGV 425
           +SG+                 DL  +G  +E   T+    V  +Q  ++ V   PK    
Sbjct: 362 ESGS-----------------DLYMDGPNLEKGPTKEMIMVVHSQPEELTV---PKK--- 421

Query: 426 LCDNSSNFSKGKAKAKVSHLTRIASDHRSLLAEWSIEPLNPSFVIPRRPRRFEEGWCKYG 485
              N+ N+ K  A+AK + +  I         + + +     F       RFE  W K  
Sbjct: 422 --TNTRNW-KRLARAKKTEINSINQSSLFRNLQMTSQHTKSRF----SSFRFETYWLKEE 481

Query: 486 ECREIVATVWNSQG-CHDISDFNTKIMDCLMSLNHWSRRKYGGSIRGAIAKTERDIQHLS 545
           +   ++   W  +G  +   DF  K+  C   L  WS+ ++  ++   I    R+I++L 
Sbjct: 482 DIGRVIMEAWKEKGPSNSAQDFICKLNQCASRLIGWSKTRF-KNLSNQITVKNREIENLY 541

Query: 546 KKDDQTSRTA-LREKEKTLESLLEDDEIYWKQRSREEWLLWGDRNTKWFHMRANTRRKRN 605
           K  ++    A ++E EKT+E LL+ +E++WKQRSR +WL  GDRN+K+FH RA+ R+K+ 
Sbjct: 542 KSCEKDGVMAVIKELEKTVEELLDCEELFWKQRSRADWLEVGDRNSKFFHARASARKKKK 563

Query: 606 RIRGIMNDLGIWTEEDNGMEFIVNNYFAKLFQSSEPQMDSIAHILESIPTSISEAQNNDL 662
               + N+ G   + + GM  ++ +YF  LFQSS P    I+   E I +  ++ Q  +L
Sbjct: 602 STHMLYNNEGRIEDTNEGMARVIQDYFHSLFQSSNPFPTDISKASECISSCFNQTQREEL 563

BLAST of Spg005683 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 7.9e-11
Identity = 82/378 (21.69%), Postives = 134/378 (35.45%), Query Frame = 0

Query: 676  DEILWNLDSKGRFSVKGAYRLGCQMNQRFQASSANYKDQEAMWKDFWKLKLPPKIKICGW 735
            D + W     G+FSV+ AY +                +  + +   WK+++P ++K   W
Sbjct: 253  DRLSWKFSQDGQFSVRSAYEM-------LTVDEVPRPNMASFFNCLWKVRVPERVKTFLW 312

Query: 736  RIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKMTKGLWAKYLPLDNLSCL 795
             + N  + T    + R +    VC +C+   E+  H+   C    G+W + +P       
Sbjct: 313  LVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQRRQQGF 372

Query: 796  FDREDRRISETL-DGLWQRGGNTSTNILHIKCSLIICWRIWSIR--NLISHNNQRLNQET 855
            F +    + E L D L  R G    +I       +I W  W  R  N+   N +      
Sbjct: 373  FSKS---LFEWLYDNLGDRSG--CEDIPWSTIFAVIIWWGWKWRCGNIFGENTK------ 432

Query: 856  IRDILQQQISASIHELIGDEEPYQMQWLEGQTERLAPSGRGGVLLG--DPTVRRK--WSP 915
                              D   +  +W       +  +  G VL+G   P V R   W  
Sbjct: 433  ----------------CRDRVKFVKEW----AVEVYRAHSGNVLVGITQPRVERMIGWVS 492

Query: 916  ISDGCWKLSYDASWRSDRECGSVGWVLRDWSRTLLAAGFKCINSASDISWLEALSIVEGL 975
               G  K++ D + R +    S G VLRD +      GF            E   +  GL
Sbjct: 493  PCVGWVKVNTDGASRGNPGLASAGGVLRDCTGA-WCGGFSLNIGRCSAPQAELWGVYYGL 552

Query: 976  -----QAIPSVTGGVRLLVENDSLQVVNLINGEDVDETELNFFIKEAQRMCSIKKVDSIT 1035
                 + +P      R+ +E DS  +V  +     D   L+F ++          +  I 
Sbjct: 553  YFAWEKKVP------RVELEVDSEVIVGFLKTGISDSHPLSFLVRLCHGFLQKDWLVRIV 585

Query: 1036 HIPRAHNYLAHSLAQKAY 1042
            H+ R  N LA  LA  A+
Sbjct: 613  HVYREANRLADGLANYAF 585

BLAST of Spg005683 vs. ExPASy TrEMBL
Match: A0A803P5M6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.3e-52
Identity = 277/1331 (20.81%), Postives = 482/1331 (36.21%), Query Frame = 0

Query: 12   KQLSGLKVTEEERASVFKLKEEEIDRSEKKLENALVCKILSQKQINPEMFKSKMSHIWNQ 71
            K  + + +TE+E  SVF+  + E       ++  L  KIL++K++     +++M+  W+ 
Sbjct: 7    KMKTKINLTEDEE-SVFQFHDTESTSPLLSVDTVLYAKILTKKKVWLSTLQNQMAEHWDG 66

Query: 72   EQTIISMVGFNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSF 131
               +      ++++  F     K  + D  P+ F    ++   P+ G      +  +  F
Sbjct: 67   RFPVKISEAMDMFMLTFGCEGDKIRVLDREPYHFQNHHIVLYTPEVGKNFTSDDLTFTPF 126

Query: 132  WIHFHKLPFACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDATKPLKRGI 191
            W+  ++LPF   +R +A  +G+I+G+   +  E+ +++  G  LRV++ +D +KPLKRG 
Sbjct: 127  WVQIYRLPFLSKTRTLAIALGNIIGEYKDV-FEDSLNEGWGPFLRVRVLLDVSKPLKRGR 186

Query: 192  FLSSEDSTEDRWIPITYEKLPDFCYGCGLLGHTLKEC-----EGSNHDGSPVEELPY--- 251
             +S     +  W+   YE+LP++C  CG++GH   +C     +  N +   +E  P+   
Sbjct: 187  MISLSHVKDKFWVDFRYERLPEYCMECGVIGHPYNKCSVFLEKLDNGEEPALEYQPFIKG 246

Query: 252  ------------------GAW-----LREPVLLKAREGGWRGGHHHDEEAYGAGGNERRQ 311
                               AW     L +  L  A     + G  H    +    +    
Sbjct: 247  STLPTSGYDRYRTDFAKGDAWPLITRLAKKSLTSAIPQLAKRGQPHPRILFSGESSNTNN 306

Query: 312  GGTEEATIRDQPSASG--PPTMNKPPA-------------NIPTG--AGPETDTMANNSD 371
                   + D  +A    P    KPP+             N+  G  A    D+  N SD
Sbjct: 307  FEDSNNNLDDIVAARSMVPKDTIKPPSVVFHPSTLLSSAQNLHKGMNAPATKDSPTNFSD 366

Query: 372  MERV-------------------EKVTELK-KVPVKEGEIK--------VSLKDNFISKG 431
            +  +                     +T+++ K P+               ++    +  G
Sbjct: 367  LSSIYMPAFVPNTTNTFATYPPQTSLTDIRGKQPMPAHATNALPPKVAPATISPAVMHWG 426

Query: 432  KVN-SPNMDEDSGN------------NGPIGKEKESYCSIMEVDLEDNGSKVEATSTEVK 491
              N +PN+     N             G I  +  S  S+ EVD       V     +  
Sbjct: 427  TENTNPNVQSKRQNEGLSLRQTLKRCRGTIPNDAASTLSVGEVD----HHLVSIDLMDSM 486

Query: 492  STQDNDVGVFLDPKGKGV---LCDNSSNFSKGKAKAK----------------------- 551
              +DN V    DP    +      +   + KG+                           
Sbjct: 487  GDKDNSVRGLSDPPHSSMKIPFHGDKFTWIKGRHNPNALKERLDWCFLNDLWETIFKPIT 546

Query: 552  VSHLTRIASDHRSLLAEWSIEPLNPSFVIPRRPR--RFEEGWCKYGECREIVATVWNSQG 611
             +HL   +SDHR++    +IE +  +   P R    RFE+ W K  +   I+   W+   
Sbjct: 547  TAHLDYYSSDHRAIAV--TIEYITSNQQQPTRKTRFRFEKIWLKEPDAASIIRAHWSDTD 606

Query: 612  CHDISDFNTKIMDCLMSLNHWSRRKYGGSIRGAIAKTERDIQHLSKKDDQTSRT--ALRE 671
               +  F + +  C  +L  W  RK+ G+++  I   ++ +  L+   D++  T   L++
Sbjct: 607  AGGMDIFLSNLQSCTDALQQWHIRKF-GNMKKKITNMQQQVSRLNNPADRSVSTMDELKD 666

Query: 672  KEKTLESLLEDDEIYWKQRSREEWLLWGDRNTKWFHMRANTRRKRNRIRGIMNDLGIWTE 731
             E  L+ LLE +E YW QRSR +WL  GD+NT +FH  A +R+ +N I+ ++N  G+   
Sbjct: 667  SEAILDELLEQEETYWHQRSRVDWLQCGDQNTSFFHAHATSRKSKNVIKSLINTQGLSVS 726

Query: 732  EDNGMEFIVNNYFAKLFQSSEPQMDSIAHILESIPTSI--------------SEAQNND- 791
                M  ++ +Y+  LF S     DS+  IL ++P SI              +E  NND 
Sbjct: 727  SKAEMTNVICDYYTSLFASDGVDPDSLNDILTTVPASITHEMNQSLTTPFTSNEVLNNDK 786

Query: 792  ------------------------------------------------------------ 851
                                                                        
Sbjct: 787  SVMSFSPNTSLAAQDFFARTLHMPITECHERYLGLPSYSGRDKQELFSSIKDKVWKLLHA 846

Query: 852  ---------------------------------------LE------------------- 911
                                                   LE                   
Sbjct: 847  WNEKIFSVGGKEVLLKAVVQSIPTYAMSCFKLTKKFCDQLERDGIHVDSGKDPWIPSHND 906

Query: 912  ------------------------------------DALAILATPTKSNMGEDEILWNLD 971
                                                D   IL+ P     G D ++W+  
Sbjct: 907  FKPVSYLGSASLPVSHFITNNRVWNVPLLTSYFQQIDIDRILSIPLAYFAGTDRLVWHHS 966

Query: 972  SKGRFSVKGAYRLGCQMNQRFQASSANYKDQEAMWKDFWKLKLPPKIKICGWRIYNDILP 1031
              G +SVK  + L   +  +  +S++N   Q   WK FW LKLPPKI+I  W+++ +ILP
Sbjct: 967  PNGIYSVKTGFHLATTLEDQNSSSTSN--KQSEWWKFFWNLKLPPKIRIFAWKVFQNILP 1026

Query: 1032 TLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKMTKGLWAKYLPLDNLSCLFDREDRRI 1055
            T   L  R +     C LC    E+  H  + CK  K +W        LS  F  +  + 
Sbjct: 1027 TAVALFKRKVLDSGECSLCTSNWESIGHALFSCKHAKDIW-------KLS-RFRIDFSQA 1086

BLAST of Spg005683 vs. ExPASy TrEMBL
Match: A0A2N9E949 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS3389 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.4e-48
Identity = 278/1204 (23.09%), Postives = 442/1204 (36.71%), Query Frame = 0

Query: 79   VGFNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKL 138
            +G NL++  F +   + L+  NGPW FDK L+L K   G      ++    SFWI  H+L
Sbjct: 16   IGDNLFIAIFNDAMDRDLVLMNGPWAFDKNLVLLKLFDGDQKVQGMQLTEASFWIQIHEL 75

Query: 139  PFACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDATKPL--KRGIFLSSE 198
            PF   + E A  +G+ LG + +ID+ E+     G  +RV+++ID + PL  ++ + L  E
Sbjct: 76   PFKGMNEETATSVGNALGVLEEIDIPED-GITWGEFMRVQVRIDVSMPLLRRQRVKLGKE 135

Query: 199  DSTEDRWIPITYEKLPDFCYGCGLLGHTLKEC-----EGSNHDGSPVEELPYGAWLREPV 258
            +S    W+ + YEKLP FCY CG+LGH+ +EC        + DG+  E   YG+WLR   
Sbjct: 136  ESI---WVTLKYEKLPTFCYNCGILGHSERECRLVTQHEKSKDGNHYE---YGSWLR--- 195

Query: 259  LLKAREGGWRGGHHHDEEAYGAGG-----NERRQGGTEEATIRDQPSAS------GPPTM 318
               A  G  + G    E A+         N+   GG + A+       S      G P  
Sbjct: 196  ---ATPGRRKTGSVTFEGAFPKRNGIVYHNQLNGGGCKAASPTAMSILSWNCQGLGNPWT 255

Query: 319  NKPPANIPTGAGPE----TDTMANNSDMERVEKVTELKKVPVKEGEI-KVSLKDNFISKG 378
             +    +     P      +T     +ME+  ++T     P + G +    L  +   + 
Sbjct: 256  VRHLHELVKENNPSILFLMETRMKAVEMEKTWRITGFYGCPERSGRLDSWKLLKHLGDQN 315

Query: 379  K---VNSPNMDEDSGNNGPIGK------EKESYCSIMEV-DLEDNGSKVEATSTEVKSTQ 438
            +   V   + +E  GN+  +GK      +  S+   +E   L D G + +  + + +   
Sbjct: 316  RLPWVVIGDFNEILGNSEKMGKALRAESQMASFREALEFHSLRDLGFRGQWYTWDNRRLG 375

Query: 439  DNDVGVFLDPKGKGVLCDNSSNFSKGKAKAKVSHLTRIASDHRSLLAEWSIEPLNPSFVI 498
              ++   LD   + V     +N     ++A V HL   ASDH  +L       L  S ++
Sbjct: 376  LENIKERLD---RAVANPEWTNIF---SEAGVQHLFTSASDHCPILLH-----LFTSILL 435

Query: 499  PRRPR--RFEEGWCKYGECREIVATVWNS--QGCHDISDFNTKIMDCLMSLNHWSRRKYG 558
            PRR R  RFE+ WC+   C E V   W+S  +G H + +    I  C   L +W R  Y 
Sbjct: 436  PRRKRQFRFEQAWCRVETCEETVKESWHSKEKGSH-MFEVTESIKSCRRGLINWERSVYR 495

Query: 559  GS-IRGAIAKTERDI--QHLSKKDDQTSRTALREKEKTLESLLEDDEIYWKQRSREEWLL 618
             S +R      E  +  +    ++DQ     LRE+   L  LLE +EI W+QRSR +WL 
Sbjct: 496  HSQLRMHQLGEEAKLLEERAVTEEDQYQLKKLREE---LNELLEREEIAWRQRSRVQWLQ 555

Query: 619  WGDRNT----------------------------KWFHMRANTRRK-------------- 678
             G+RNT                                +RA   +K              
Sbjct: 556  EGNRNTNRRGGRGGFTKRCIQMYPTKAPGPDEGLSALLVRAEQEKKIQGVKVARAAPAIS 615

Query: 679  ------------------------------------------------------------ 738
                                                                        
Sbjct: 616  HLFFADDSILFCQATREACTTIQEILDTYEVASERVWKKIQGWKEKLLSKASKEVLIKAV 675

Query: 739  --------------------RNRIRGIMNDLGIWT---------------EEDNGMEFIV 798
                                   +RG+  DLGIW                + +N +  +V
Sbjct: 676  AQAVPTYAMSVFKLPFGLKFAKDVRGVDWDLGIWNGSIKAMLAKMGWRLLQNENSLVHMV 735

Query: 799  --NNYF-------AKLFQSSEPQMDSIA-------------------------------- 858
                YF       A + Q S     SIA                                
Sbjct: 736  LKAKYFAGGTFMQATVKQGSSFTWRSIADARWVLEEGLRWRIGNGTRVSVWGDKWLPSGS 795

Query: 859  ---------------HILESIPTSISEAQNNDL------EDALAILATPTKSNMGEDEIL 918
                            + E I   I +   N L      ED   IL  P    + ED  L
Sbjct: 796  TNRPITPKSQDMVDPKVSELIQGEIGQWDVNKLQLWFMPEDVATILTIPISRALPEDIQL 855

Query: 919  WNLDSKGRFSVKGAYRLGCQMNQRFQASSANYKDQEAMWKDFWKLKLPPKIKICGWRIYN 978
            WN    G+F+V  AYRL  + N   Q  S++      +W+  WK++LP  IK+  WR  +
Sbjct: 856  WNATPHGKFTVSSAYRLIAERNNLAQGESSSGYPMAKVWRAVWKVQLPNNIKVFTWRALH 915

Query: 979  DILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKMTKGLWAKYLPLDNLSCLFDRE 1038
            + LPTL NL  R +     C  C+ + ET  H  W C      W++     N    F   
Sbjct: 916  NALPTLVNLERRKISQSIWCPRCKLQAETVLHAIWECDEVANCWSESTMAVNYKLHFGDF 975

Query: 1039 DRRISETLDGLWQRGGNTSTNILHIKCSLIICWRIWSIRNLISHNNQRLNQETIRDILQQ 1041
             + + E  +     G         ++  + I ++IW  RN ++        E I     Q
Sbjct: 976  TQLVWEIFEKQGVEG---------LETFMSISYQIWVSRNPLAF-------EGILMAPSQ 1035

BLAST of Spg005683 vs. ExPASy TrEMBL
Match: A0A803QPR1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.1e-46
Identity = 246/1157 (21.26%), Postives = 438/1157 (37.86%), Query Frame = 0

Query: 22   EERASVFKLKEEEIDRSEKKLENALVCKILSQKQINPEMFKSKMSHIWNQEQTI-ISMVG 81
            EE      L +++ D SE      LV + L+++ I+ +  + +M+ +W   + + +  + 
Sbjct: 10   EEDVDRLVLDDDDGDLSEIDDRWCLVGRFLTKRSIDCQAIQHRMASLWQPNRGLYVKELD 69

Query: 82   FNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKLPF 141
             N +L +F +    + + D  PW FD+  L+F+  K G     I    +  WI  H +  
Sbjct: 70   PNHFLFQFYHEIGITRVIDGSPWTFDRVPLIFERVKPGENPRQISLTRLDVWIQCHDMKT 129

Query: 142  ACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDATKPLKRGIFLSSEDSTE 201
               S      + + +G   + D        R   LRV+  I+  KPLK+ + L  ++  +
Sbjct: 130  GFMSENTLKHMANYIGTFMKSDPNNFTGVWR-DYLRVRTTINVEKPLKKKMTLERKNG-Q 189

Query: 202  DRWIPITYEKLPDFCYGCGLLGHT--------------LKECEGSNHDGSP--VEELPYG 261
               +   YE LP FC+ CG+LGH+              +K+    +   +P   +     
Sbjct: 190  TCTVQFKYEDLPTFCFICGVLGHSKWFYDRLFDTLAKVIKKMFDLSLKAAPRRRQHTIGS 249

Query: 262  AWLREPVLLKAREGGWRGGHHHDEEAYGA--GGNERRQG--------GTEEATIRDQPSA 321
             WLR      +  G   GG    +        G E  QG        G  +  IR   S 
Sbjct: 250  PWLRSGA---SYRGPVTGGGESSQAGVSRNFNGEESNQGLIANLHNIGNPQQLIRGDVSH 309

Query: 322  SGPPTMNKPPANIPTGAGPETDTMANNSDMERVEKVTELKKVPVKEGEIKVSLKD----N 381
                       +I  G    ++ + +N+ +  VE       V    GE  V   D    +
Sbjct: 310  LLEKDPKAKDKSIQGGNDVASEDILSNNALLVVENKRRRTDVEKGLGESVVDQMDGSNLS 369

Query: 382  FISKGKVNSPN-----MDEDSGNNGPIGKEKESYCSIMEVDLEDNGSKVEATSTEVKSTQ 441
             I  G++++ N     +D+D+  +  +     S+  +  V  E N ++ E T T +K+  
Sbjct: 370  SIGLGRISNDNGIFEMLDQDNHIDMKVATLDGSWWRLTGVYGEPNRNEREQTWTLLKTLT 429

Query: 442  DN---------DVGVFLDPKGK-------------------------------------- 501
             N         D+   L  + K                                      
Sbjct: 430  RNSTLPWCVIGDLNNVLSQEDKKGGRPYPQWLLQGFQSCLDECGLEDLELLGYPFTWERA 489

Query: 502  -------GVLCDNSSNFSKGKA---KAKVSHLTRIASDHRSLLAEWSIEPL-NPSFVIPR 561
                    V  D +   +  +    +A +++L    SDH  +     +EP+   SF  PR
Sbjct: 490  RGTREWIEVRLDRALVTNSWRTLFLEACLTNLEASTSDHCPIF----LEPIVRVSFRTPR 549

Query: 562  RPRRFEEGWCKYGECREIVATVWNSQGCHDISDFNTKIMDCLMSLNHWSRRKYGGSIRGA 621
            R  RFE  W     C ++V   W++Q     S    KI  C+  L  W  ++  G  +  
Sbjct: 550  R-FRFENAWLSEPMCYQLVQDCWDTQSNDSTSG---KISMCVEVLGRWG-KEITGDFKSR 609

Query: 622  IAKTERDIQHLSKKDDQTSRTALREKEKTLESLLEDDEIYWKQRSREEWLLWGDRNTKWF 681
            I + +  +  L  K D  S     E    L  +LE  E +WKQR+++ WL  GD+N+K+F
Sbjct: 610  IKQCKTTMSILKPKMDAESVKQYAEARNRLFEVLEQQETFWKQRAKQFWLKGGDKNSKYF 669

Query: 682  HMRANTRRKRNRIRGIMNDLGIWTEEDNGMEFIVNNYFAKLFQSSEPQMDSIAHILESIP 741
            H  A++R++ N I  + +D G W + +N +  ++N+Y+  LF ++         +++S+ 
Sbjct: 670  HKTASSRKQHNTILQLKDDHGTWVDWNNRLANVINSYYTSLFATANTHCQ---EVIDSVS 729

Query: 742  TSISEAQNNDLEDAL-------AILATPTKSNMGEDEI---------------------- 801
              +    N +L   +       A+       + G D +                      
Sbjct: 730  AIVPSHANEELSKPVSEEGVRKAVFQMHPDKSPGPDGMTPAFYQKCWQIVKRDVTRTVQH 789

Query: 802  ----------LWNLDSKGRFSVKGAYRLGCQMNQRFQASSANYKDQ--EAMWKDFWKLKL 861
                       W+ ++   FSVK AY +        Q    N+ D   +  W+  W+LKL
Sbjct: 790  FFEHGVLEDGFWSKENSSTFSVKSAYAV-------LQKLKGNWFDDAGDVFWRKLWQLKL 849

Query: 862  PPKIKICGWRIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKMTKGLWAKY 921
            P K++   WR  N+ L T+  L  + +DV P+C +C  + ET  H    C+  +  W   
Sbjct: 850  PSKMQNLVWRACNNCLSTVVQLRTKRVDVSPLCPICHNEHETILHALVTCREVRKCW--- 909

Query: 922  LPLDNLSCLFDREDRRISETLDGLWQRGGNTS--TNILHIKCSL-IICWRIWSIRNLISH 981
                      DR     S T    +    + +   N   + C + ++CW +W  RN +  
Sbjct: 910  ----------DRVGFGTSVTPGASFFDWCSVAFRNNSATVNCRIVVVCWAVWGARNNLVW 969

Query: 982  NNQRLNQETIRDILQQQISASIHELIGDEEPYQMQWLEGQTERLAPSGRGGVLLGDPTVR 1041
            N +                     +  D   Y  ++L+        S     +L    V 
Sbjct: 970  NKKPF-------------------IFEDVVAYAFRYLDQWKSAQVSSNSSWPILTAADVG 1029

BLAST of Spg005683 vs. ExPASy TrEMBL
Match: A0A2N9F5Y8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS10420 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.3e-43
Identity = 180/711 (25.32%), Postives = 300/711 (42.19%), Query Frame = 0

Query: 34  EIDRSEKKLENALVCKILSQKQINPEMFKSKMSHIWNQEQTIISM-VGFNLYLCKFKNGK 93
           ++   +++ +  L  K  +++ IN E        +W  ++   +  +G N+ L +F++  
Sbjct: 22  QLSTPDEERKPTLAAKFFTRRVINIEAVTRTFKPLWRSDRGFSARDLGENMVLFEFEDSA 81

Query: 94  IKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKLPFACFSREVAAEIG 153
               +  + PW +DK+L+ F++ +  +  + I     +FW+  H LP     +++A  +G
Sbjct: 82  DVERVLLHEPWSYDKSLVAFRKLEAEDEPESIVLDRATFWVQIHNLPVIALKKDIALALG 141

Query: 154 SILGQVCQIDLEEEVDQCRGG--TLRVKIQIDATKPLKRGIFLSSEDSTEDRWIPITYEK 213
             +G V +     E D+  GG   +RV++Q++ +KPL RG  L   +  E RWI   YE+
Sbjct: 142 KSMGTVVR---TSEADEEFGGGRIMRVRVQVELSKPLCRGCKLVLANGGE-RWISFKYER 201

Query: 214 LPDFCYGCGLLGHTLKECEG--SNHDGSPVEELPYGAWLREPVLLKAREGGWRGGHHHDE 273
           LP FCY CGLL H  K+C+   +N +    E+  YG WLR  +     E  +R      +
Sbjct: 202 LPKFCYWCGLLSHREKDCKFWLNNQETLSREDQGYGTWLRAEL-----ERSFR------K 261

Query: 274 EAYGAGGNERRQGGTEEATIRDQPSASGPPTMNKPPANIPTGAGPETDTMANNSDME--- 333
                 G  R +    E+  + Q  A  PPT   PP +  +G     D        E   
Sbjct: 262 TEIKVPGRSRPKKPPPESVDKQQKEAPPPPTEMVPPRDEDSGDMECEDISEFPKQGELFL 321

Query: 334 ---RVEKVTELKKVPVKEGEIKVSLKDNFISKG--------KVNSPNMDEDSGNNGPIGK 393
                     L+ + ++ G       D +   G         +  P    ++ +  PI  
Sbjct: 322 ASLHTTFEEHLRMIDLEIGFCPAHPVDPYAPVGPPKIQDHATIGLPQSSSNNESTRPIIN 381

Query: 394 EKESY-------CSIMEVDLEDNGS---KVEATSTEVKSTQ------------------D 453
           + E Y        S   +    NG+   K +A +    S Q                  +
Sbjct: 382 KLEVYPLGDLTNMSPSPLPQRKNGTGTWKKKARAHGSNSDQPPLITLDKRSWDDENQAPN 441

Query: 454 NDVGVFLDPKGKGVLCDNSSNFSKGK-----------AKAKVSHLTRIASDHRSLLAEWS 513
            D G++    G       S  ++  K              +V H+    SDH+ L   W 
Sbjct: 442 EDGGIY----GASETSRRSDTWTMLKRLYRANPLPWCVIGRVEHVNVTNSDHKCLCMSWD 501

Query: 514 IEPLNPSFVIPRRPRRFEEGWCKYGECREIVATVWNSQGCHDISDFN--TKIMDCLMSLN 573
                P+    R+P  F+E W +   C E +   WN       + F   TKI  C  SL 
Sbjct: 502 C----PTNKRHRKPFHFKEMWLEDMGCEETIRETWNPDP-RGTTMFRVATKINQCKKSLG 561

Query: 574 HWSRRKYGGSIRGAIAKTERDIQHLS-KKDDQTSRTALREKEKTLESLLEDDEIYWKQRS 633
            WSRR + GSIR  + + ++ ++             A +     +  LL+ +E  W+QRS
Sbjct: 562 DWSRRNF-GSIRRQLVEKKKQLEDAEIMAMTGGGLEAAKSLRIEINELLKKEEKMWRQRS 621

Query: 634 REEWLLWGDRNTKWFHMRANTRRKRNRIRGIMNDLGIWTEEDNGMEFIVNNYFAKLFQSS 677
           R  WL  GDRNTK+FH RA+ RR+RN I+ + +  GIW E ++ +  +  +YF  LF +S
Sbjct: 622 RSTWLKEGDRNTKYFHGRASQRRRRNTIKRVRDSAGIWQENEDQVARVFLDYFRTLFTTS 681

BLAST of Spg005683 vs. ExPASy TrEMBL
Match: A0A2Z6NZV1 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_140560 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.1e-43
Identity = 176/735 (23.95%), Postives = 294/735 (40.00%), Query Frame = 0

Query: 22  EERASVFKLKEEEIDRSEKKLENALVCKILSQKQINPEMFKSKMSHIWNQEQTI-ISMVG 81
           E+      ++ EEI   E+  +  LV K+ ++   N   FK  ++  W  + +I +  + 
Sbjct: 12  EDEEDCITVEAEEICEEEETFKRTLVGKLWTENPYNVRAFKQTIAQAWRLKNSIEVQDLE 71

Query: 82  FNLYLCKFKNGKIKSLIADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKLPF 141
            NL+L +F   K    +  NGPW FD+ LL+     G     D++   V+FW+  + LPF
Sbjct: 72  ENLFLFRFTTKKDADTVLRNGPWSFDRNLLILHRVSGEEQPSDLDMHNVNFWVRVYDLPF 131

Query: 142 ACFSREVAAEIGSILGQVCQIDLEEEVDQCRGGTLRVKIQIDATKPLKRGIFLSSEDSTE 201
              S  +A ++G+I+G   ++D ++      G  LR+K  +D  KPLKRG  +  +D  +
Sbjct: 132 KLRSEAMAKKLGNIMGNFEEVDPKDA--NRTGRFLRLKASLDLRKPLKRGTKIKFQD--K 191

Query: 202 DRWIPITYEKLPDFCYGCGLLGHTLKECEG------SNHDGSPVEELPYGAWLREPVLLK 261
           + W+   YE+LP+FC+ CG +GH +KECE       +N+     +   YG WLR   L +
Sbjct: 192 NMWVDFKYERLPNFCFACGKIGHQMKECEDVEDVDENNYSDIAEKSQAYGPWLRASPLPR 251

Query: 262 AREGGWRGGHHHDEEAYGAGGNERRQGGTEEATIRDQPSASGPPTMNKPPANIPTGAGPE 321
             E   +              + + +GG  E     +      P +      +PT A   
Sbjct: 252 IFEEPRKDASSGSCSKNLFPSSSQSKGGQSEGKKDKEQEVDQQPVVGIEKQLVPTKATNN 311

Query: 322 TD----------------------------------TMANNSDMERVEKVTELKKVPVKE 381
            D                                      +S   + +   +L K   K 
Sbjct: 312 LDVEDVAESLGAVAISTSFVVGKTTKSQGGSKGRKWVRNKSSKPVKAQTARKLAKELGKR 371

Query: 382 GEIKVSLKD---------------NFISKGKVNSPNMDEDSGNNG----------PIGKE 441
             + V++ +                F+ + ++  P +D      G           +G+E
Sbjct: 372 NLVDVAISEVRALSRLIRLENPQVVFLMETRLKVPEIDRLKFKLGFSSGLAIDCKGVGRE 431

Query: 442 KESYCSIMEVDLEDNGSK------VEATSTEVKSTQDNDV-GVFLDPKGKGVL------- 501
           +    ++   D  D   K      +     +V++ +  D+ G++ + K  G++       
Sbjct: 432 RAGGLALFWKDHMDITIKSYSLNHIHGQCVDVETNEPWDLTGIYAEEKKGGLVRTQGQLE 491

Query: 502 ---------------------------------------CDNSSNFSKGKAKAKVSHLTR 561
                                                    +S  F    +   V HL R
Sbjct: 492 IGRQAILESRLNDLGFEGYPLTWSNGRNSDDNKQCRQDRAMSSDEFINRFSPIHVLHLPR 551

Query: 562 IASDHRSLLAEWSIEPLNPSFVIPRRPR--RFEEGWCKYGECREIVATVWNSQGCHDISD 621
             SDH  L+               RR R  RFEE W     C  ++   W SQ C   SD
Sbjct: 552 YGSDHVVLVITLEAPTHRDQRRRRRRKRLFRFEESWTSNARCEPLIQACW-SQPCLSFSD 611

Query: 622 FNTKIMDCLMSLNHWSRRKYGGSIRGAIAKTERDIQHLSKKDD-QTSRTALREKEKTLES 635
              ++ D    L   S     GSI   I + E+ IQ+    D+ +TS    +  E TLE 
Sbjct: 612 KLGRLRDMGNELGDHS----VGSIHKEIVRIEKLIQNHDMWDESETSIQRFKALEHTLEE 671

BLAST of Spg005683 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 6.4e-16
Identity = 80/320 (25.00%), Postives = 139/320 (43.44%), Query Frame = 0

Query: 722  WKLKLPPKIKICGWRIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKMTKG 781
            WKLK  PKIK   W++ +  L T  NL  R +   P C  C +++ET+ HLF+ C   + 
Sbjct: 19   WKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYAQQ 78

Query: 782  LW-AKYLPLDNLSCLFDREDRRISETLDGLWQRGGNTSTNILHIKCSLIICWRIWSIRNL 841
            +W A  +P   L       + ++   L        N    + ++  ++ I WR+W  RN 
Sbjct: 79   VWRASGIPHQELRTTGITMETKMELLLSSCL---ANRQPQLFNL--AIWILWRLWKSRNQ 138

Query: 842  ISHNNQRLN-QETIRDILQQQISASIHELIGDEEPYQMQWLEGQTERLAPSGRGGVLLGD 901
            +    + ++ Q T+     Q+    + E   ++    +Q L  Q      S R       
Sbjct: 139  LVFQQKSISWQNTL-----QRARNDVQE--WEDTNTYVQSLNQQVH----SSRH----QQ 198

Query: 902  PTV-RRKWSPISDGCWKLSYDASWRSDRECGSVGWVLRDWSRTLLAAGFKCINSASDISW 961
            PT+ R KW        K +YD ++         GW++RD +   + +G    ++ SD   
Sbjct: 199  PTMARTKWQRPPSTWIKYNYDGAFNHQTRNAKAGWLMRDENGVYMGSGQAIGSTTSDSLE 258

Query: 962  LEALSIVEGLQAIPSVTGGVRLLVENDSLQVVNLINGEDVDETELNFFIKEAQRMCSIKK 1021
             E  +++  +Q   S  G  +++ E DS QV  L+N E ++    N +I+E +      +
Sbjct: 259  SEFQALIIAMQHAWS-QGYRKVIFEGDSKQVEELMNNEKLNFGRFN-WIREGRFWQKRFE 316

Query: 1022 VDSITHIPRAHNYLAHSLAQ 1039
                  +PR +N  A  LA+
Sbjct: 319  EAVFKWVPRTNNQPADILAK 316

BLAST of Spg005683 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 3.2e-15
Identity = 88/405 (21.73%), Postives = 158/405 (39.01%), Query Frame = 0

Query: 676  DEILWNLDSKGRFSVKGAYRLGCQ-MNQRFQASSANYKDQEAMWKDFWKLKLPPKIKICG 735
            D   W+  S G ++VK  Y +  Q +N+R      +      +++  WK +  PKI+   
Sbjct: 211  DSYTWDYTSSGDYTVKSGYWVLTQIINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFL 270

Query: 736  WRIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKMTKGLWA-KYLPLDNLS 795
            W+  ++ LP    L  R +     C  C   +ET +HL + C   +  WA   +P+    
Sbjct: 271  WKCLSNSLPVAGALAYRHLSKESACIRCPSCKETVNHLLFKCTFARLTWAISSIPIP--- 330

Query: 796  CLFDREDRRISETLDGLWQRGGNTSTNILHIKCSLII---CWRIWSIRNLISHNNQRLNQ 855
             L       I   L  ++  G     N    K S ++    WR+W  RN +    +  N 
Sbjct: 331  -LGGEWADSIYVNLYWVFNLGNG---NPQWEKASQLVPWLLWRLWKNRNELVFRGREFNA 390

Query: 856  ETIRDILQQQISASIHELIGDEEPYQMQWLEGQTERLAPSGRGGVLLGDPTVRR----KW 915
            + +           +     D E +++     +TE  +   +       P V R    +W
Sbjct: 391  QEV-----------LRRAEDDLEEWRI-----RTEAESCGTK-------PQVNRSSCGRW 450

Query: 916  SPISDGCWKLSYDASWRSDRECGSVGWVLRDWSRTLLAAGFKCINSASDISWLEALSIVE 975
             P      K + DA+W  D E   +GWVLR              N   ++ W+ A ++ +
Sbjct: 451  RPPPHQWVKCNTDATWNRDNERCGIGWVLR--------------NEKGEVKWMGARALPK 510

Query: 976  GLQAIPSVTGGVR-------------LLVENDSLQVVNLINGEDVDETELNFFIKEAQRM 1035
                + +    +R             ++ E+DS  ++ ++N +++    L   I++ QR+
Sbjct: 511  LKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNNDEI-WPSLKPTIQDLQRL 569

Query: 1036 CSIKKVDSITHIPRAHNYLAHSLAQK--AYEEDGPKSWSHSFPDW 1057
             S         IPR  N LA  +A++  ++    PK +S   P W
Sbjct: 571  LSQFTEVKFVFIPREGNTLAERVARESLSFLNYDPKLYS-IVPSW 569

BLAST of Spg005683 vs. TAIR 10
Match: AT3G42140.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 54.3 bits (129), Expect = 7.1e-07
Identity = 31/144 (21.53%), Postives = 51/144 (35.42%), Query Frame = 0

Query: 97  IADNGPWFFDKALLLFKEPKGGNYGDDIEFRYVSFWIHFHKLPFACFSREVAAEIGSILG 156
           I   GPW F+  + + +  +      D EF+ + FWI    +P    +  +   IG    
Sbjct: 75  ILRRGPWSFNDWMCVIQ--RWTKLHSDAEFKRIPFWIQIRGIPLRFLTARIITSIGE--- 134

Query: 157 QVCQIDLEEEVDQCRGGTLRVKIQIDATKPLKRGIFLSSEDSTEDRWIPITYEKLPDFCY 216
                                          + G+FL +    +   +   YEKL +FC 
Sbjct: 135 -------------------------------RMGLFLETNLGRDVSVLKFQYEKLKNFCT 182

Query: 217 GCGLLGHTLKECEGSNHDGSPVEE 241
            CG+L H   EC  S + G   ++
Sbjct: 195 TCGMLSHDASECPTSGNQGPHADD 182

BLAST of Spg005683 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 53.9 bits (128), Expect = 9.3e-07
Identity = 22/57 (38.60%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 720 DFWKLKLPPKIKICGWRIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHC 777
           D W LK+ PKIK+  W+  N+ LP  + L +R + + P C  CR+  ET +H+ ++C
Sbjct: 8   DIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCRD-FETITHILFNC 63

BLAST of Spg005683 vs. TAIR 10
Match: AT1G60720.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-05
Identity = 42/148 (28.38%), Postives = 60/148 (40.54%), Query Frame = 0

Query: 719 KDFWKLKLPPKIKICGWRIYNDILPTLSNLNNRGMDVWPVCFLCREKEETTSHLFWHCKM 778
           K  W     PK     W    D LPT   L + G      C LC  + E+  HL + C+ 
Sbjct: 129 KAVWFKGAVPKHAFNMWISQLDRLPTRQRLASWGHIQSFDCCLCTIETESRDHLLFSCEF 188

Query: 779 TKGLW----AKYLPLDNLSCLFDREDRRISETLDGLWQRGGNTSTNILHIKCSL-IICWR 838
              +W    ++  P   L C +       +E L   W R  ++S   L  K S   I + 
Sbjct: 189 AAQVWRLAFSRLCPRQRLFCSW-------AELLS--WMRSSSSSAPSLLRKVSAHAIIYN 248

Query: 839 IWSIRNLISHNNQRLNQETIRDILQQQI 862
           IW  RN + HNN R+    I  I+ ++I
Sbjct: 249 IWRQRNNVLHNNLRIAPIIIFKIVDREI 267