Spg005368 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg005368
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	transmembrane emp24 domain-containing protein p24beta2-like
Location	scaffold6: 37187306 .. 37187900 (+)
RNA-Seq Expression	Spg005368
Synteny	Spg005368

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAATTGGAAAGTTTATTTTAATTGTGGTTTTTTTGGTTTCACAAAAGGCTGTGTTTGGGATTCGATTTGTGATCGACAAAGAAGAATGCTACTCTGAAAACGTTGAATTTGTTAGAGATACAATTCATCTCTCTTTTGTTGTTGTTAGTGTTCATACAGCTTCTTGGCGACATGCTCACAAAGGCGTTCATCTTTTAGTGACAAACTTTTACAAATTTTGGGAGTGTTTCTAAAAACTGAGAAACAAGAAACATACATTGTTATTAAATGCTTAGATTTCTTAATAAAATTGGAAACAATAAACAAGAAACGAGAACGTTACCAAACGGGCCCTTAATTTCTACTTTTCTCTTAGATTAATATCCATCTTAGATTGTTGTAATTTGGAAAGTAGAGGTGAAGGGGCCTTTAGGGGAGGGGATTGAAGAAATAAGATGGAGGACAAGTGAGAAGTTGAAGTTTGTAGCTAATACAAAAGGCCAATATCGCTTCTGCTTCACCAACAGACCATTGAATTTGATATTCATATCAGCCGTTATTATGAATTCTAAGATCATCATCATCAACATTAACCCTATACTTTTACCTTGA

mRNA sequence

ATGAGAATTGGAAAGTTTATTTTAATTGTGGTTTTTTTGGTTTCACAAAAGGCTGTGTTTGGGATTCGATTTGTGATCGACAAAGAAGAATGCTACTCTGAAAACGTTGAATTTGTTAGAGATACAATTCATCTCTCTTTTGTTGTTGTTAGTGTTCATACAGCTTCTTGGCGACATGCTCACAAAGGCGTTCATCTTTTAGTGACAAACTTTTACAAATTTTGGGAGTTAGAGGTGAAGGGGCCTTTAGGGGAGGGGATTGAAGAAATAAGATGGAGGACAAGTGAGAAGTTGAAGTTTGTAGCTAATACAAAAGGCCAATATCGCTTCTGCTTCACCAACAGACCATTGAATTTGATATTCATATCAGCCGTTATTATGAATTCTAAGATCATCATCATCAACATTAACCCTATACTTTTACCTTGA

Coding sequence (CDS)

ATGAGAATTGGAAAGTTTATTTTAATTGTGGTTTTTTTGGTTTCACAAAAGGCTGTGTTTGGGATTCGATTTGTGATCGACAAAGAAGAATGCTACTCTGAAAACGTTGAATTTGTTAGAGATACAATTCATCTCTCTTTTGTTGTTGTTAGTGTTCATACAGCTTCTTGGCGACATGCTCACAAAGGCGTTCATCTTTTAGTGACAAACTTTTACAAATTTTGGGAGTTAGAGGTGAAGGGGCCTTTAGGGGAGGGGATTGAAGAAATAAGATGGAGGACAAGTGAGAAGTTGAAGTTTGTAGCTAATACAAAAGGCCAATATCGCTTCTGCTTCACCAACAGACCATTGAATTTGATATTCATATCAGCCGTTATTATGAATTCTAAGATCATCATCATCAACATTAACCCTATACTTTTACCTTGA

Protein sequence

MRIGKFILIVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWRHAHKGVHLLVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTNRPLNLIFISAVIMNSKIIIININPILLP

Homology

[Close]

BLAST of Spg005368 vs. NCBI nr
Match: XP_022144018.1 (transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia])

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-28
Identity = 79/122 (64.75%), Postives = 87/122 (71.31%), Query Frame = 0


Query: 1   MRIGKF-----ILIVVFL--VSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVH 60
           MRIGK      I +V  L  VS +AVFG RFVIDKEEC+S NVEF RDTIHLSFVVVSVH
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TASWRHAHKGVHLLVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFT 116
            ASWRHAH GV           +L VKGP GEGIEE + +TSEK KFVA+ KG YRFCFT
Sbjct: 61  -ASWRHAHNGV-----------DLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFT 110

[Close]

BLAST of Spg005368 vs. NCBI nr
Match: KAG6596756.1 (Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028293.1 Transmembrane emp24 domain-containing protein p24beta2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 123.6 bits (309), Expect = 1.3e-24
Identity = 67/110 (60.91%), Postives = 77/110 (70.00%), Query Frame = 0


Query: 6   FILIVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWR-HAHKGV 65
           FI +    +S   V GIRFVIDKEEC+S NVEF+RDTI+LSFVVVSVHT  WR HAH GV
Sbjct: 13  FIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHT--WRHHAHNGV 72

Query: 66  HLLVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTN 115
            LL           VKGPLGE IEEIR +TSEK +F++  KG+Y FCF N
Sbjct: 73  DLL-----------VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFIN 109

[Close]

BLAST of Spg005368 vs. NCBI nr
Match: XP_022952151.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata])

HSP 1 Score: 120.9 bits (302), Expect = 8.7e-24
Identity = 65/107 (60.75%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 9   IVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWR-HAHKGVHLL 68
           +    +S   V GIRFVIDKEEC+S NVEF+RDTI+LSFVVVSVHT  WR HAH GV LL
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHT--WRHHAHNGVDLL 60

Query: 69  VTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTN 115
                      VKGPLGE IEEIR +TSEK +F++  KG+Y FCF N
Sbjct: 61  -----------VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFIN 94

[Close]

BLAST of Spg005368 vs. NCBI nr
Match: XP_023539502.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 120.9 bits (302), Expect = 8.7e-24
Identity = 65/107 (60.75%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 9   IVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWR-HAHKGVHLL 68
           +    +S   V GIRFVIDKEEC+S NVEF+RDTI+LSFVVVSVHT  WR HAH GV LL
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHT--WRHHAHNGVDLL 60

Query: 69  VTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTN 115
                      VKGPLGE IEEIR +TSEK +F++  KG+Y FCF N
Sbjct: 61  -----------VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFIN 94

[Close]

BLAST of Spg005368 vs. NCBI nr
Match: XP_023005965.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima])

HSP 1 Score: 118.2 bits (295), Expect = 5.6e-23
Identity = 64/107 (59.81%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 9   IVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWR-HAHKGVHLL 68
           +    +S   V GIRFVIDKEEC+S NVEF+RDTI+LSFVVVSV+T  WR HAH GV LL
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNT--WRHHAHNGVDLL 60

Query: 69  VTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTN 115
                      VKGPLGE IEEIR +TSEK +F++  KG+Y FCF N
Sbjct: 61  -----------VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFIN 94

[Close]

BLAST of Spg005368 vs. ExPASy Swiss-Prot
Match: Q9S7M9 (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.0e-15
Identity = 46/109 (42.20%), Postives = 62/109 (56.88%), Query Frame = 0


Query: 7   ILIVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWRHAHKGVHL 66
           I+++  L S +A  GIRFVID+EEC+S   E+  DT+H+SFVV+    + W     GV  
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIK-SDSQWHFNEDGV-- 66

Query: 67  LVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTNR 116
                    +L + GP GE I + R + S K  FV   KG YRFCFTN+
Sbjct: 67  ---------DLVIHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNK 103

[Close]

BLAST of Spg005368 vs. ExPASy TrEMBL
Match: A0A6J1CS53 (transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charantia OX=3673 GN=LOC111013807 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 5.7e-29
Identity = 79/122 (64.75%), Postives = 87/122 (71.31%), Query Frame = 0


Query: 1   MRIGKF-----ILIVVFL--VSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVH 60
           MRIGK      I +V  L  VS +AVFG RFVIDKEEC+S NVEF RDTIHLSFVVVSVH
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TASWRHAHKGVHLLVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFT 116
            ASWRHAH GV           +L VKGP GEGIEE + +TSEK KFVA+ KG YRFCFT
Sbjct: 61  -ASWRHAHNGV-----------DLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFT 110

[Close]

BLAST of Spg005368 vs. ExPASy TrEMBL
Match: A0A6J1GJG3 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschata OX=3662 GN=LOC111454883 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 4.2e-24
Identity = 65/107 (60.75%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 9   IVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWR-HAHKGVHLL 68
           +    +S   V GIRFVIDKEEC+S NVEF+RDTI+LSFVVVSVHT  WR HAH GV LL
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHT--WRHHAHNGVDLL 60

Query: 69  VTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTN 115
                      VKGPLGE IEEIR +TSEK +F++  KG+Y FCF N
Sbjct: 61  -----------VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFIN 94

[Close]

BLAST of Spg005368 vs. ExPASy TrEMBL
Match: A0A6J1KYW1 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima OX=3661 GN=LOC111498823 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.7e-23
Identity = 64/107 (59.81%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 9   IVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWR-HAHKGVHLL 68
           +    +S   V GIRFVIDKEEC+S NVEF+RDTI+LSFVVVSV+T  WR HAH GV LL
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNT--WRHHAHNGVDLL 60

Query: 69  VTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTN 115
                      VKGPLGE IEEIR +TSEK +F++  KG+Y FCF N
Sbjct: 61  -----------VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFIN 94

[Close]

BLAST of Spg005368 vs. ExPASy TrEMBL
Match: A0A6J5XME6 (GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34477 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 7.4e-21
Identity = 57/109 (52.29%), Postives = 74/109 (67.89%), Query Frame = 0


Query: 7   ILIVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWRHAHKGVHL 66
           +L++ FLVS +AVFGIRFVID+EEC+S NV+F  DT+H+SFVV+ V  ASW +   GV  
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQFEGDTVHISFVVIKV-DASWHYTQDGV-- 70

Query: 67  LVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTNR 116
                    +L VKGP GE I + R +TSEK  FV   KG ++FCFTN+
Sbjct: 71  ---------DLVVKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNK 107

[Close]

BLAST of Spg005368 vs. ExPASy TrEMBL
Match: A0A6J5V7G0 (GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS35037 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 7.4e-21
Identity = 57/109 (52.29%), Postives = 74/109 (67.89%), Query Frame = 0


Query: 7   ILIVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWRHAHKGVHL 66
           +L++ FLVS +AVFGIRFVID+EEC+S NV+F  DT+H+SFVV+ V  ASW +   GV  
Sbjct: 79  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQFEGDTVHISFVVIKV-DASWHYTQDGV-- 138

Query: 67  LVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTNR 116
                    +L VKGP GE I + R +TSEK  FV   KG ++FCFTN+
Sbjct: 139 ---------DLVVKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNK 175

[Close]

BLAST of Spg005368 vs. TAIR 10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.4e-16
Identity = 46/109 (42.20%), Postives = 62/109 (56.88%), Query Frame = 0


Query: 7   ILIVVFLVSQKAVFGIRFVIDKEECYSENVEFVRDTIHLSFVVVSVHTASWRHAHKGVHL 66
           I+++  L S +A  GIRFVID+EEC+S   E+  DT+H+SFVV+    + W     GV  
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIK-SDSQWHFNEDGV-- 66

Query: 67  LVTNFYKFWELEVKGPLGEGIEEIRWRTSEKLKFVANTKGQYRFCFTNR 116
                    +L + GP GE I + R + S K  FV   KG YRFCFTN+
Sbjct: 67  ---------DLVIHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNK 103

The following BLAST results are available for this feature:

BLAST of Spg005368 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144018.1	1.2e-28	64.75	transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia...	[more]
KAG6596756.1	1.3e-24	60.91	Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyr...	[more]
XP_022952151.1	8.7e-24	60.75	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata]	[more]
XP_023539502.1	8.7e-24	60.75	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subs...	[more]
XP_023005965.1	5.6e-23	59.81	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima]	[more]

BLAST of Spg005368 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7M9	2.0e-15	42.20	Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O...	[more]

BLAST of Spg005368 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CS53	5.7e-29	64.75	transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charant...	[more]
A0A6J1GJG3	4.2e-24	60.75	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschat...	[more]
A0A6J1KYW1	2.7e-23	59.81	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima ...	[more]
A0A6J5XME6	7.4e-21	52.29	GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34...	[more]
A0A6J5V7G0	7.4e-21	52.29	GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS35037...	[more]

BLAST of Spg005368 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07680.1	1.4e-16	42.20	emp24/gp25L/p24 family/GOLD family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR22811:SF143	EMP24/GP25L/P24 FAMILY/GOLD PROTEIN	coord: 5..115
IPR015720	Transmembrane emp24 domain-containing protein	PANTHER	PTHR22811	TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN	coord: 5..115
IPR009038	GOLD domain	PROSITE	PS50866	GOLD	coord: 29..131 score: 9.368151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg005368.1	Spg005368.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046907	intracellular transport
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0012505	endomembrane system
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0016020	membrane