Spg005309 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg005309
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsalutaridinol 7-O-acetyltransferase-like
Locationscaffold11: 34719743 .. 34720639 (-)
RNA-Seq ExpressionSpg005309
SyntenySpg005309
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTGTCTTCTGTACCGAGCCAATCGAGAAACATGCTCAAATCGTCGTTCAAGCCAACGTCTTCGACTGCGGTGGAATCGCAATCTCTTTATGTCTATTGCACAAGCTCATAGACGTAACAACTCTCAGCTGTTTTCTAAGATCTTGGGCTTCCACCAACAAAGACTCTCTCAATTCATCTCCAAAGATGTCATGCTCCGACTACAAAGCCTTCTCCTCACTCTTCCCACAAACAAACTTGTTGCCTTTCCATACCCAAGTACTCCATTCGAGGTCCCAAATCCAACAAGCGTCGAAGCACTAACTGGGTTTATTTGGAAACATGCAATGAAAGCCTCAACGATTGGAACTTGCTCTCGAAGTGAAAAGCTGTCGACTTTATCGCACGTAGTGAACTTGAGAAAGAGAATGGAGCCGTCGTTGCCCGAAGTTTCTGTGGGAAACGTGATGTGGGGAGCAGTCGCTCATTACAATTCAGCGAACGAATCGATCGAAGTGAAGCTAAGCGAGCTGGTGGGATTATTGAGACAATCGTTTGCAAAAATCAACAGTGATTTCGTAAAAGGGTTGATGGGAGATAAAGGAACGAGAGGCGTTTTGAAGCTGATTGGCGAGATGAATAAGTTTCCATCTGAAGTTCGAGATTACTACTTTTTCACGAGTTGGCGAAACATGGATTTGAATGAAGTTGATTTTGGATGGGGAAAGCCTGCATGGATTGGAATTGCAGGAGACTTGGAAGAGATCATGACGAATATTATTGTTCTTTTGGATACTGTGTCGGGAGATGGAGTGGAGGCTTGGATGGTATTGGATGAAAAGGAAATGAAATTTTTAGAACAAAATCCACAGTTTCTCAAGTTTGCATTGCCAAATCCGAGTGTTGCAATTTAG

mRNA sequence

ATGTTCTGTCTTCTGTACCGAGCCAATCGAGAAACATGCTCAAATCGTCGTTCAAGCCAACGTCTTCGACTGCGGTGGAATCGCAATCTCTTTATGTCTATTGCACAAGCTCATAGACGTAACAACTCTCAGCTGTTTTCTAAGATCTTGGGCTTCCACCAACAAAGACTCTCTCAATTCATCTCCAAAGATGTCATGCTCCGACTACAAAGCCTTCTCCTCACTCTTCCCACAAACAAACTTGTTGCCTTTCCATACCCAAGTACTCCATTCGAGGTCCCAAATCCAACAAGCGTCGAAGCACTAACTGGGTTTATTTGGAAACATGCAATGAAAGCCTCAACGATTGGAACTTGCTCTCGAAGTGAAAAGCTGTCGACTTTATCGCACGTAGTGAACTTGAGAAAGAGAATGGAGCCGTCGTTGCCCGAAGTTTCTGTGGGAAACGTGATGTGGGGAGCAGTCGCTCATTACAATTCAGCGAACGAATCGATCGAAGTGAAGCTAAGCGAGCTGGTGGGATTATTGAGACAATCGTTTGCAAAAATCAACAGTGATTTCGTAAAAGGGTTGATGGGAGATAAAGGAACGAGAGGCGTTTTGAAGCTGATTGGCGAGATGAATAAGTTTCCATCTGAAGTTCGAGATTACTACTTTTTCACGAGTTGGCGAAACATGGATTTGAATGAAGTTGATTTTGGATGGGGAAAGCCTGCATGGATTGGAATTGCAGGAGACTTGGAAGAGATCATGACGAATATTATTGTTCTTTTGGATACTGTGTCGGGAGATGGAGTGGAGGCTTGGATGGTATTGGATGAAAAGGAAATGAAATTTTTAGAACAAAATCCACAGTTTCTCAAGTTTGCATTGCCAAATCCGAGTGTTGCAATTTAG

Coding sequence (CDS)

ATGTTCTGTCTTCTGTACCGAGCCAATCGAGAAACATGCTCAAATCGTCGTTCAAGCCAACGTCTTCGACTGCGGTGGAATCGCAATCTCTTTATGTCTATTGCACAAGCTCATAGACGTAACAACTCTCAGCTGTTTTCTAAGATCTTGGGCTTCCACCAACAAAGACTCTCTCAATTCATCTCCAAAGATGTCATGCTCCGACTACAAAGCCTTCTCCTCACTCTTCCCACAAACAAACTTGTTGCCTTTCCATACCCAAGTACTCCATTCGAGGTCCCAAATCCAACAAGCGTCGAAGCACTAACTGGGTTTATTTGGAAACATGCAATGAAAGCCTCAACGATTGGAACTTGCTCTCGAAGTGAAAAGCTGTCGACTTTATCGCACGTAGTGAACTTGAGAAAGAGAATGGAGCCGTCGTTGCCCGAAGTTTCTGTGGGAAACGTGATGTGGGGAGCAGTCGCTCATTACAATTCAGCGAACGAATCGATCGAAGTGAAGCTAAGCGAGCTGGTGGGATTATTGAGACAATCGTTTGCAAAAATCAACAGTGATTTCGTAAAAGGGTTGATGGGAGATAAAGGAACGAGAGGCGTTTTGAAGCTGATTGGCGAGATGAATAAGTTTCCATCTGAAGTTCGAGATTACTACTTTTTCACGAGTTGGCGAAACATGGATTTGAATGAAGTTGATTTTGGATGGGGAAAGCCTGCATGGATTGGAATTGCAGGAGACTTGGAAGAGATCATGACGAATATTATTGTTCTTTTGGATACTGTGTCGGGAGATGGAGTGGAGGCTTGGATGGTATTGGATGAAAAGGAAATGAAATTTTTAGAACAAAATCCACAGTTTCTCAAGTTTGCATTGCCAAATCCGAGTGTTGCAATTTAG

Protein sequence

MFCLLYRANRETCSNRRSSQRLRLRWNRNLFMSIAQAHRRNNSQLFSKILGFHQQRLSQFISKDVMLRLQSLLLTLPTNKLVAFPYPSTPFEVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWMVLDEKEMKFLEQNPQFLKFALPNPSVAI
Homology
BLAST of Spg005309 vs. NCBI nr
Match: XP_022138192.1 (BAHD acyltransferase BIA1-like [Momordica charantia])

HSP 1 Score: 306.2 bits (783), Expect = 3.1e-79
Identity = 146/209 (69.86%), Postives = 177/209 (84.69%), Query Frame = 0

Query: 92  EVPNPTSVEALTGFIWKHAM--KASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGN 151
           EVPNPTSVEALTGFIW  AM  KA+++G    S++ STLSHVVNLRKRMEP LPE+S+GN
Sbjct: 250 EVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEPPLPEISIGN 309

Query: 152 VMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIGEMNK 211
           V+WG VAHY S+N   +V+L +LV LLRQSF+KIN+DF+KGLMG+ G R VL+L+GE+NK
Sbjct: 310 VLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAVLELLGEINK 369

Query: 212 FPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAW 271
           FPS+V +YYFFTSWRNMDLNEVDFGWGKP WIGIAG+   I  + I+LLDT SGDG+EAW
Sbjct: 370 FPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAW 429

Query: 272 MVLDEKEMKFLEQNPQFLKFALPNPSVAI 299
           MVLD+K+MK L+QNPQFL+FA  NPS++I
Sbjct: 430 MVLDQKKMKLLQQNPQFLEFASLNPSISI 458

BLAST of Spg005309 vs. NCBI nr
Match: KAG6582063.1 (Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.5 bits (672), Expect = 2.3e-66
Identity = 147/258 (56.98%), Postives = 180/258 (69.77%), Query Frame = 0

Query: 43  SQLF--SKILGFHQQRLSQFISKDVMLRLQSLLLTLPTNKLVAFPYPSTPFEVPNPTSVE 102
           S LF  + +L FHQ+       K  + R         T  L+     +    VPNP+ VE
Sbjct: 190 SSLFPQTNLLPFHQKLAKPANRKRCLRR-----FVFGTKSLLNLKAKAESRAVPNPSCVE 249

Query: 103 ALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMWGAVAHYNS 162
           ALTGFIWKHAM A+    C  S   STLSHVVNLR RM+P+LPEVSVGN+MWGAVAH +S
Sbjct: 250 ALTGFIWKHAMMAA---DCQGS---STLSHVVNLRGRMKPALPEVSVGNIMWGAVAH-SS 309

Query: 163 ANESIEVKLSELVGLLRQSFAKINSDFVKGLM--GDKGTRGVLKLIGEMNKFPSEVRDYY 222
           A E +E  L  LVGLLRQSF +IN D+++ ++   DKG   VLK +GEMNK+   + +YY
Sbjct: 310 AMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEMNKW--GMSNYY 369

Query: 223 FFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWMVLDEKEMK 282
           F TSWRNM LNEVDFGWG+PAWI IAGD  E+M N+IVL+D V GDG+EAW++LDEKEM+
Sbjct: 370 FCTSWRNMKLNEVDFGWGRPAWIAIAGDTNELMANVIVLVDGVMGDGIEAWILLDEKEME 429

Query: 283 FLEQNPQFLKFALPNPSV 297
            LEQNPQFL+FAL NPS+
Sbjct: 430 ILEQNPQFLEFALLNPSI 431

BLAST of Spg005309 vs. NCBI nr
Match: KAG6582064.1 (Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.5 bits (672), Expect = 2.3e-66
Identity = 136/206 (66.02%), Postives = 162/206 (78.64%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           VPNP+ VEALTGFIWKHAM A+    C  S   STLSHVVNLR RM+P+LPEVSVGN+MW
Sbjct: 237 VPNPSCVEALTGFIWKHAMMAA---DCQGS---STLSHVVNLRGRMKPALPEVSVGNIMW 296

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLM--GDKGTRGVLKLIGEMNKF 212
           GAVAH +SA E +E  L  LVGLLRQSF +IN D+++ ++   DKG   VLK +GEMNK+
Sbjct: 297 GAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEMNKW 356

Query: 213 PSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWM 272
              +  YYF TSWRNM LNEVDFGWG+PAWI IAGD  E+M N+IVL+D V GDG+EAW+
Sbjct: 357 --GMSKYYFCTSWRNMKLNEVDFGWGRPAWIAIAGDTNELMANVIVLVDGVMGDGIEAWI 416

Query: 273 VLDEKEMKFLEQNPQFLKFALPNPSV 297
           +LDEKEM+ LEQNPQFL+FAL NPS+
Sbjct: 417 LLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Spg005309 vs. NCBI nr
Match: XP_022956051.1 (salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata])

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-65
Identity = 135/206 (65.53%), Postives = 162/206 (78.64%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           VPNP+ VEALTGFIWKHAM A+    C  S   STLSHVVNLR RM+P+LPEVSVGN+MW
Sbjct: 237 VPNPSCVEALTGFIWKHAMMAA---DCQGS---STLSHVVNLRGRMKPALPEVSVGNIMW 296

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLM--GDKGTRGVLKLIGEMNKF 212
           GAVAH +SA E +E  L  LVGLLRQSF +IN D+++ ++   DKG   VLK +GEMNK+
Sbjct: 297 GAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEMNKW 356

Query: 213 PSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWM 272
              + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+EAW+
Sbjct: 357 --GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIEAWI 416

Query: 273 VLDEKEMKFLEQNPQFLKFALPNPSV 297
           +LDEKEM+ LEQNPQFL+FAL NPS+
Sbjct: 417 LLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Spg005309 vs. NCBI nr
Match: XP_022956252.1 (salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata])

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-65
Identity = 135/206 (65.53%), Postives = 162/206 (78.64%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           VPNP+ VEALTGFIWKHAM A+    C  S   STLSHVVNLR RM+P+LPEVSVGN+MW
Sbjct: 237 VPNPSCVEALTGFIWKHAMMAA---DCQGS---STLSHVVNLRGRMKPALPEVSVGNIMW 296

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLM--GDKGTRGVLKLIGEMNKF 212
           GAVAH +SA E +E  L  LVGLLRQSF +IN D+++ ++   DKG   VLK +GEMNK+
Sbjct: 297 GAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEMNKW 356

Query: 213 PSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWM 272
              + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+EAW+
Sbjct: 357 --GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIEAWI 416

Query: 273 VLDEKEMKFLEQNPQFLKFALPNPSV 297
           +LDEKEM+ LEQNPQFL+FAL NPS+
Sbjct: 417 LLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Spg005309 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 9.7e-20
Identity = 64/221 (28.96%), Postives = 112/221 (50.68%), Query Frame = 0

Query: 79  NKLVAFPYPSTPFEVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRM 138
           +K+    + +    VP PT VEA+   IW+ A  +S         + + +   +++R R+
Sbjct: 221 SKIAELKHKAASESVPVPTRVEAIMSLIWRCARNSSRSNLL--IPRQAVMWQAMDIRLRI 280

Query: 139 EPSL-PEVSVGNVMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGT 198
             S+ P+  +GN+  G     ++ +   E ++ E+V   R++  ++N    + L G+   
Sbjct: 281 PSSVAPKDVIGNLQSGFSLKKDAES---EFEIPEIVATFRKNKERVNEMIKESLQGNTIG 340

Query: 199 RGVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNI--I 258
           + +L L+ E     +E+ D Y  +SW      EVDFG G P W+G A     I  N+  +
Sbjct: 341 QSLLSLMAETVSESTEI-DRYIMSSWCRKPFYEVDFGSGSPVWVGYAS--HTIYDNMVGV 400

Query: 259 VLLDTVSGDGVEAWMVLDEKEMKFLEQNPQFLKFALPNPSV 297
           VL+D+  GDGVEAW+ L E++M     + + L +A+ NP V
Sbjct: 401 VLIDSKEGDGVEAWISLPEEDMSVFVDDQELLAYAVLNPPV 433

BLAST of Spg005309 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.4e-18
Identity = 62/196 (31.63%), Postives = 103/196 (52.55%), Query Frame = 0

Query: 88  STPFEVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSV 147
           S+  EV N + V+ +   IWK  +  +     ++++ ++  +  VNLR RM P  P+ ++
Sbjct: 235 SSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVA--AQAVNLRSRMNPPFPQSAM 294

Query: 148 GNVMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIGEM 207
           GN+   A   Y  A E  +   S+LVG L+ S AKI+ + VK L      +GV  L    
Sbjct: 295 GNI---ATMAYAVAEE--DKDFSDLVGPLKTSLAKIDDEHVKELQ-----KGVTYL---- 354

Query: 208 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 267
             + +E ++ + F+SW  +   ++DFGWGKP  +         M N++ L+DT + DG+E
Sbjct: 355 -DYEAEPQELFSFSSWCRLGFYDLDFGWGKPVSVCTT---TVPMKNLVYLMDTRNEDGME 410

Query: 268 AWMVLDEKEMKFLEQN 284
           AW+ + E EM  L  +
Sbjct: 415 AWISMAEDEMSMLSSD 410

BLAST of Spg005309 vs. ExPASy Swiss-Prot
Match: Q9FI40 (BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.5e-17
Identity = 62/226 (27.43%), Postives = 107/226 (47.35%), Query Frame = 0

Query: 78  TNKLVAFPYPSTPFEVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKR 137
           ++K+      +    VP+PT VEA+   IW+ A  AS          + T S  + LR  
Sbjct: 223 SDKITKLKIVAASKSVPSPTRVEAVMSLIWRCARNASHANLIVPRATMMTQSMDLRLRIP 282

Query: 138 MEPSLPEVSVGNVMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGT 197
                P+ ++GN+       +       E+++SE+V   R+   + N + +K  +    T
Sbjct: 283 TNVLSPD-AIGNLQG---VFFLKRGPGSEIEISEVVAEFRKEKEEFN-EMIKENVNGGHT 342

Query: 198 RGVL--KLIGEMNKFPSEVR---DYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMT 257
              L  K++  +  + SE++   D Y  +SW      EVDFGWG+PAW+G+     +   
Sbjct: 343 NTTLGQKIMSGIANYMSELKPNIDTYTMSSWCRKAFYEVDFGWGRPAWVGLGHQDIQDGV 402

Query: 258 NIIVLLDTVSGDGVEAWMVLDEKEMKFLEQNPQFLKFALPNPSVAI 299
             ++L+D   G+GVE W+ + E++M     + + L +A  NP V I
Sbjct: 403 MYVLLVDAKDGEGVEVWVGIPEQDMAAFVCDQELLSYASLNPPVLI 443

BLAST of Spg005309 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.5e-17
Identity = 62/202 (30.69%), Postives = 98/202 (48.51%), Query Frame = 0

Query: 97  TSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMWGAVA 156
           T VE +T  IWK  MK++  G       L  + H VNLRK+M+P L +VS GN+     A
Sbjct: 271 TRVEVVTSLIWKSVMKSTPAGF------LPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSA 330

Query: 157 HY--------NSANESIEVKLSE---LVGLLRQSFAKINSDFVKGLMGDKGT--RGVLKL 216
                     N+ N++I    SE   ++  L    A++ S+  K + GDKG+  + +   
Sbjct: 331 FLPATTTTTTNAVNKTINSTSSESQVVLHELHDFIAQMRSEIDK-VKGDKGSLEKVIQNF 390

Query: 217 IGEMNKFPSEVRDY----YFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLD 276
               +    ++ D     ++ +SW  M L E+DFGWGKP W+ +  +++    N   + D
Sbjct: 391 ASGHDASIKKINDVEVINFWISSWCRMGLYEIDFGWGKPIWVTVDPNIKP-NKNCFFMND 450

Query: 277 TVSGDGVEAWMVLDEKEMKFLE 282
           T  G+G+E W    E +M   E
Sbjct: 451 TKCGEGIEVWASFLEDDMAKFE 464

BLAST of Spg005309 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 5.0e-16
Identity = 60/193 (31.09%), Postives = 93/193 (48.19%), Query Frame = 0

Query: 88  STPFEVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSV 147
           S+  E  N + V+ +  +IWKH +  +     ++++    +   VNLR RM P LP  ++
Sbjct: 234 SSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNK--FVVVQAVNLRSRMNPPLPHYAM 293

Query: 148 GNVMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIGEM 207
           GN+   A   + + +   +    +L+G LR S  K   D    L+  KG   + +L    
Sbjct: 294 GNI---ATLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDDHNHELL--KGMTCLYEL---- 353

Query: 208 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 267
                E ++   FTSW  +   ++DFGWGKP     A        N  +L+DT SGDGVE
Sbjct: 354 -----EPQELLSFTSWCRLGFYDLDFGWGKPL---SACTTTFPKRNAALLMDTRSGDGVE 407

Query: 268 AWMVLDEKEMKFL 281
           AW+ + E EM  L
Sbjct: 414 AWLPMAEDEMAML 407

BLAST of Spg005309 vs. ExPASy TrEMBL
Match: A0A6J1CAD9 (BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.5e-79
Identity = 146/209 (69.86%), Postives = 177/209 (84.69%), Query Frame = 0

Query: 92  EVPNPTSVEALTGFIWKHAM--KASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGN 151
           EVPNPTSVEALTGFIW  AM  KA+++G    S++ STLSHVVNLRKRMEP LPE+S+GN
Sbjct: 250 EVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEPPLPEISIGN 309

Query: 152 VMWGAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIGEMNK 211
           V+WG VAHY S+N   +V+L +LV LLRQSF+KIN+DF+KGLMG+ G R VL+L+GE+NK
Sbjct: 310 VLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAVLELLGEINK 369

Query: 212 FPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAW 271
           FPS+V +YYFFTSWRNMDLNEVDFGWGKP WIGIAG+   I  + I+LLDT SGDG+EAW
Sbjct: 370 FPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAW 429

Query: 272 MVLDEKEMKFLEQNPQFLKFALPNPSVAI 299
           MVLD+K+MK L+QNPQFL+FA  NPS++I
Sbjct: 430 MVLDQKKMKLLQQNPQFLEFASLNPSISI 458

BLAST of Spg005309 vs. ExPASy TrEMBL
Match: A0A6J1GVI6 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111457861 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 5.5e-66
Identity = 135/206 (65.53%), Postives = 162/206 (78.64%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           VPNP+ VEALTGFIWKHAM A+    C  S   STLSHVVNLR RM+P+LPEVSVGN+MW
Sbjct: 237 VPNPSCVEALTGFIWKHAMMAA---DCQGS---STLSHVVNLRGRMKPALPEVSVGNIMW 296

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLM--GDKGTRGVLKLIGEMNKF 212
           GAVAH +SA E +E  L  LVGLLRQSF +IN D+++ ++   DKG   VLK +GEMNK+
Sbjct: 297 GAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEMNKW 356

Query: 213 PSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWM 272
              + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+EAW+
Sbjct: 357 --GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIEAWI 416

Query: 273 VLDEKEMKFLEQNPQFLKFALPNPSV 297
           +LDEKEM+ LEQNPQFL+FAL NPS+
Sbjct: 417 LLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Spg005309 vs. ExPASy TrEMBL
Match: A0A6J1GVT5 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111458004 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 5.5e-66
Identity = 135/206 (65.53%), Postives = 162/206 (78.64%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           VPNP+ VEALTGFIWKHAM A+    C  S   STLSHVVNLR RM+P+LPEVSVGN+MW
Sbjct: 237 VPNPSCVEALTGFIWKHAMMAA---DCQGS---STLSHVVNLRGRMKPALPEVSVGNIMW 296

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLM--GDKGTRGVLKLIGEMNKF 212
           GAVAH +SA E +E  L  LVGLLRQSF +IN D+++ ++   DKG   VLK +GEMNK+
Sbjct: 297 GAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEMNKW 356

Query: 213 PSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWM 272
              + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+EAW+
Sbjct: 357 --GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIEAWI 416

Query: 273 VLDEKEMKFLEQNPQFLKFALPNPSV 297
           +LDEKEM+ LEQNPQFL+FAL NPS+
Sbjct: 417 LLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Spg005309 vs. ExPASy TrEMBL
Match: A0A0A0KXH2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 9.3e-66
Identity = 135/213 (63.38%), Postives = 168/213 (78.87%), Query Frame = 0

Query: 92  EVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVM 151
           ++PNPT VEA+T FIWK+ MK +  G    S+  STLSHVVN+RK +EPSL EVS+GN+M
Sbjct: 260 DIPNPTCVEAITCFIWKYLMKVADGGD---SQMPSTLSHVVNIRKMIEPSLGEVSLGNIM 319

Query: 152 WGAVAHYNSA---NESIE-VKLSELVGLLRQSFAKINSDFVKGLM--GDKGTR-GVLKLI 211
           WG VAH+ S    NES E ++LS+LV LLRQS  KIN D++K L+  GDKG R GV+KL+
Sbjct: 320 WGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYIKELIMGGDKGRRNGVMKLV 379

Query: 212 GEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGD 271
           GE+NK+P  + +YYFFTSW+NM LNE+DFGWGKP W GIAGD  E+M NIIVL+D VS D
Sbjct: 380 GEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFGIAGDSNEMMGNIIVLVDNVSDD 439

Query: 272 G-VEAWMVLDEKEMKFLEQNPQFLKFALPNPSV 297
           G +EAW++LDEKEM+ LEQNPQFL+FAL NPS+
Sbjct: 440 GSIEAWILLDEKEMQLLEQNPQFLEFALLNPSI 467

BLAST of Spg005309 vs. ExPASy TrEMBL
Match: A0A1S3BXK4 (LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494516 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 2.1e-62
Identity = 131/212 (61.79%), Postives = 163/212 (76.89%), Query Frame = 0

Query: 92  EVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVM 151
           ++PNPT VE LT FIWK+ MK +  G    S++ STLSHVVN+RK +EPSL EVS+GN+M
Sbjct: 296 DIPNPTCVETLTCFIWKYLMKVADDGD---SQRPSTLSHVVNIRKMLEPSLGEVSLGNIM 355

Query: 152 WGAVAHYNSA--NESIE-VKLSELVGLLRQSFAKINSDFVKGLM--GDKGTR-GVLKLIG 211
           WG VAH+ S   NE  E ++LS+LV LLRQSF KIN D++K L+  GDK  R GV+KL+G
Sbjct: 356 WGTVAHHFSTTRNEEFEGLELSKLVSLLRQSFKKINKDYIKELIMGGDKERRNGVMKLVG 415

Query: 212 EMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDG 271
           E+NK+P  + +YYFFTSW+N+ LNEVDFGWGKP W  IAGD  E+M NIIVL+D V  DG
Sbjct: 416 EINKWP--ISNYYFFTSWKNLKLNEVDFGWGKPLWSAIAGDPNEMMGNIIVLVDNVLDDG 475

Query: 272 -VEAWMVLDEKEMKFLEQNPQFLKFALPNPSV 297
             EAW++LDEKEM+ LEQ PQFL+FAL NPS+
Sbjct: 476 STEAWILLDEKEMQLLEQIPQFLEFALLNPSI 502

BLAST of Spg005309 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 129.0 bits (323), Expect = 6.2e-30
Identity = 82/224 (36.61%), Postives = 123/224 (54.91%), Query Frame = 0

Query: 78  TNKLVAFPYPSTPFEVPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKR 137
           ++ + A    ++ FEV  PT VEA++  IWK AMKA+   T S + K S L++ V+LR R
Sbjct: 221 SSSIQALQAKASSFEVNQPTRVEAVSALIWKSAMKATR--TVSGTSKPSILANSVSLRSR 280

Query: 138 MEPSLPEVSVGNVMWGAVAHYNSANESI-EVKLSELVGLLRQSFAKINSDFVKGLMGDKG 197
           + P   + S+GN++    A    A E I + KL  LV  +R++  +     +  L+G+  
Sbjct: 281 VSPPFTKNSIGNLVSYFAA---KAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPN 340

Query: 198 TRGVL----KLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMT 257
              ++    K  G+M    S   D+Y F+S     L E DFGWGKP W+G     ++   
Sbjct: 341 ATEIICSYQKEAGDM--IASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQK--- 400

Query: 258 NIIVLLDTVSGDGVEAWMVLDEKEMKFLEQNPQFLKFALPNPSV 297
           NI+ LLDT    G+EAW+ L+E+EM   EQ+ + L+FA  NPSV
Sbjct: 401 NIVTLLDTKEAGGIEAWVNLNEQEMNLFEQDRELLQFASLNPSV 434

BLAST of Spg005309 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 114.8 bits (286), Expect = 1.2e-25
Identity = 73/204 (35.78%), Postives = 110/204 (53.92%), Query Frame = 0

Query: 97  TSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVM-WGAV 156
           T VE L+ FIW   M AST     ++ K+ TL H VNLR++ +P +P+   GN+M +   
Sbjct: 237 TRVEVLSVFIWSRFM-AST-NHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFSVT 296

Query: 157 AHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIG-EMNKFPSEV 216
                 NE+ E K S LV  +R+   KI++ +VK L  D   RG L+ +  + + F +  
Sbjct: 297 VPMMIINENDEEKAS-LVDQMREEIRKIDAVYVKKLQED--NRGHLEFLNKQASGFVNGE 356

Query: 217 RDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWMVLDE 276
              + FTS     + E DFGWGKP W+  A        N++  +DT  GDG+EAW+ LD+
Sbjct: 357 IVSFSFTSLCKFPVYEADFGWGKPLWVASA---RMSYKNLVAFIDTKEGDGIEAWINLDQ 416

Query: 277 KEMKFLEQNPQFLKFALPNPSVAI 299
            +M   E + + L++   NPSV +
Sbjct: 417 NDMSRFEADEELLRYVSSNPSVMV 432

BLAST of Spg005309 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 112.8 bits (281), Expect = 4.6e-25
Identity = 67/211 (31.75%), Postives = 109/211 (51.66%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           V  PT VE++T  IWK  + +S   TC        L  + NLR ++   L E S+GN+M+
Sbjct: 244 VDQPTRVESVTALIWKCFVASSKTTTCDH----KVLVQLANLRSKIPSLLQESSIGNLMF 303

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFV-KGLMGDK----GTRGVLKLIGEM 212
            +V    S     EVK+ E V  LR+   ++ +  + +G   D     G++    ++   
Sbjct: 304 SSVV--LSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLMLTNY 363

Query: 213 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 272
           ++   E  + Y  +SW  + L E  FGW  P W  + G++  ++ N+ +L+D+  G G+E
Sbjct: 364 SRLSYETHEPYTVSSWCKLPLYEASFGWDSPVW--VVGNVSPVLGNLAMLIDSKDGQGIE 423

Query: 273 AWMVLDEKEMKFLEQNPQFLKFALPNPSVAI 299
           A++ L E+ M   EQNP+ L FA  NPSV +
Sbjct: 424 AFVTLPEENMSSFEQNPELLAFATMNPSVLV 446

BLAST of Spg005309 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 112.1 bits (279), Expect = 7.9e-25
Identity = 72/208 (34.62%), Postives = 107/208 (51.44%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           VP PT V+++T  IWK  + AST      + +   L    NLR ++   L E  +GN+++
Sbjct: 232 VPRPTRVQSVTSLIWKCVVTAST-----DTIREKALFQPANLRTKIPSLLSENQIGNLLF 291

Query: 153 GAVAHYNSANESIEVKLSELVGLLRQSFAKINSDFVKGLMGDKGTRGVLKLIGEM--NKF 212
            ++     A   I   + EL     Q  A+  S  V+   G   T G  +L GE+  +KF
Sbjct: 292 NSLILDGKAGMDIVETVKEL-----QKRAEDLSGLVQDEEGSSMTIG-WRLFGEIINSKF 351

Query: 213 PSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWM 272
             E  D +  TSW  + L +  FGWG P W  +AG +   +  + VL+D+  G G+EAW+
Sbjct: 352 NREDHDMHSVTSWCKIPLYDACFGWGSPVW--VAGSVSPNLDYVTVLIDSKDGQGIEAWV 411

Query: 273 VLDEKEMKFLEQNPQFLKFALPNPSVAI 299
            L ++ M  LEQ+ + L FA PNPSV I
Sbjct: 412 TLPQENMLLLEQSTELLAFASPNPSVLI 426

BLAST of Spg005309 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 105.5 bits (262), Expect = 7.4e-23
Identity = 68/212 (32.08%), Postives = 108/212 (50.94%), Query Frame = 0

Query: 93  VPNPTSVEALTGFIWKHAMKASTIGTCSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMW 152
           V  P  VE++T  +WK  + A++  T +   K+  L    NLR ++   L E  +GN+M+
Sbjct: 241 VGRPKRVESVTALLWKAFVAAASSNTNACDMKV--LIQPSNLRPKIPSLLAESLIGNIMF 300

Query: 153 GAVAHYNSANESIEVKLSELVGLLR------QSFAKINSDFVKGLMGDKGTRGVLKLIGE 212
            +V    S  E  E+K+ + V  LR      Q+  K        ++G K       ++  
Sbjct: 301 SSVVLGISQQE--EIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLAN---LMLTN 360

Query: 213 MNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGV 272
            +K   E  + Y  +SW  + L E  FGWG P W  IAG++  ++ N+ +L+D+  G G+
Sbjct: 361 YSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVW--IAGNVAPMLENVTMLIDSKDGQGI 420

Query: 273 EAWMVLDEKEMKFLEQNPQFLKFALPNPSVAI 299
           EA++ L E+ M  LEQN + L FA  NPSV +
Sbjct: 421 EAFVTLPEENMLSLEQNTELLAFASVNPSVLV 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138192.13.1e-7969.86BAHD acyltransferase BIA1-like [Momordica charantia][more]
KAG6582063.12.3e-6656.98Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG6582064.12.3e-6666.02Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022956051.11.1e-6565.53salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata][more]
XP_022956252.11.1e-6565.53salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O233939.7e-2028.96BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1[more]
A0A2P1GIW71.4e-1831.63Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q9FI401.5e-1727.43BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=... [more]
Q94FT43.5e-1730.69Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
Q70PR75.0e-1631.09Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CAD91.5e-7969.86BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE... [more]
A0A6J1GVI65.5e-6665.53salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1GVT55.5e-6665.53salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A0A0KXH29.3e-6663.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1[more]
A0A1S3BXK42.1e-6261.79LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX... [more]
Match NameE-valueIdentityDescription
AT3G26040.16.2e-3036.61HXXXD-type acyl-transferase family protein [more]
AT1G24430.11.2e-2535.78HXXXD-type acyl-transferase family protein [more]
AT4G15390.14.6e-2531.75HXXXD-type acyl-transferase family protein [more]
AT5G47950.17.9e-2534.62HXXXD-type acyl-transferase family protein [more]
AT3G30280.17.4e-2332.08HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 90..286
e-value: 1.6E-30
score: 106.1
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 61..288
e-value: 1.8E-48
score: 166.5
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 92..296
NoneNo IPR availablePANTHERPTHR31623:SF36VINORINE SYNTHASE-LIKEcoord: 92..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg005309.1Spg005309.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups