Spg005307 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg005307
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiondisease resistance protein RGA2-like
Locationscaffold11: 34119708 .. 34121030 (+)
RNA-Seq ExpressionSpg005307
SyntenySpg005307
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAATTCCTGTGGAGTTTTGCTGTTCAGGAAGTCTTGAAGAAGATAGTGAAGCTGGCGGCCGAGCAGATCGGTTTGGCATGGGGTTTCAAGAAGGAGCTCTCAAAGCTCAAGGACTCTCTACTCATGGTGGAAGCCATCCTTCACGACGTCCACAAAATCAAGCTAGACCATCAAGCTGTGAAGCTATGGGTGGAGAAGCTCGAAGATGTCGTCTTCGAAGCCGATGTCTTGCTCGACAAGCTCGCTTACGACGATCTCCGACGCAAGGTCGTCGGAACCGGAAAAGAGGCCATGGTAAGTTACTTCGTTTCATCTTCCAAAAACCCCCTTGTTTTTCGCCTCAAAATGGCCAATAGAATCAAGGCCGTTGCCAAAGCCTTACACGAGCATTACCGTGCAGCAAGTGTTGTGGGGCTCGTTGCTTTGACATCCAAAGATGTTGAAACTGAACTCAGCCAGATTCAAGAGACAGACTCCTTTTTGGATGAGATTGGAGTCATTGGAAGAGAAATTGAAGTATGTGAGATTGTGAACAAGCTCATTGACCTCAGTAGTCAAGAAGCTTTGTCTGTTCTACCCATTGTTGGTGAGGGTGGACTAGGGAAAACAGCTTTGGCAAAGGTAATCTTCAATCATGAGGTGATAAGGGAGAATTTTGATACAACTATATGGGTGTATGTGTCTAGACATTTTGTCATCAAGAAGATCTTGAAAGCTATTTTGGAAACTCTCAATGCCAATTTTGGTGGGTTGGATAGCAAGGAAGCCATACTCCAAGAGCTCAAAAAATCGTTGAGTGACAAAAGATATTTTCTTGTGCTCGATGATGTTTGGAATGAGAATCCGAACGTGTGGAATGAGCTAAAGGGTTGTTTGTTAAAAGTTAATAAGAACTTTGGGAGTGTGATTGTTGTCACTACTAGGAGCGATGAAGTTGCGAAAATCGTGGAGACACAATGTTGTAGGCATGATTTGAGAAAGTTATCAAATGATCAGTGTTGGTCTTTGTTTGAGAAATGTATATTTGCTAATGAATTGCCAAAAACTTTGAAGATGGATAAGGTTCGAGAGGAGCTTATTGAACAATTTGGTGGCATGCCACTAGTTGTGAAAGCATTGGGAGGAATGGTGAAATTGGACAAGAATCATGAAGAATTGGAGTCCACGTTGAAAAATCTAATTAGAATTCCTTCCAATGAAAATCACGCTTTATCTACGATGAAGTTAAGTGTGGATCGCTTACCATCATCTTCGTTAAAACAATCTTTCGCCTATCGTTCAAAATTTTCTCAAGATTTCAAGTTCAAGAAAGAATAG

mRNA sequence

ATGGCGGAATTCCTGTGGAGTTTTGCTGTTCAGGAAGTCTTGAAGAAGATAGTGAAGCTGGCGGCCGAGCAGATCGGTTTGGCATGGGGTTTCAAGAAGGAGCTCTCAAAGCTCAAGGACTCTCTACTCATGGTGGAAGCCATCCTTCACGACGTCCACAAAATCAAGCTAGACCATCAAGCTGTGAAGCTATGGGTGGAGAAGCTCGAAGATGTCGTCTTCGAAGCCGATGTCTTGCTCGACAAGCTCGCTTACGACGATCTCCGACGCAAGGTCGTCGGAACCGGAAAAGAGGCCATGGTAAGTTACTTCGTTTCATCTTCCAAAAACCCCCTTGTTTTTCGCCTCAAAATGGCCAATAGAATCAAGGCCGTTGCCAAAGCCTTACACGAGCATTACCGTGCAGCAAGTGTTGTGGGGCTCGTTGCTTTGACATCCAAAGATGTTGAAACTGAACTCAGCCAGATTCAAGAGACAGACTCCTTTTTGGATGAGATTGGAGTCATTGGAAGAGAAATTGAAGTATGTGAGATTGTGAACAAGCTCATTGACCTCAGTAGTCAAGAAGCTTTGTCTGTTCTACCCATTGTTGGTGAGGGTGGACTAGGGAAAACAGCTTTGGCAAAGGTAATCTTCAATCATGAGGTGATAAGGGAGAATTTTGATACAACTATATGGGTGTATGTGTCTAGACATTTTGTCATCAAGAAGATCTTGAAAGCTATTTTGGAAACTCTCAATGCCAATTTTGGTGGGTTGGATAGCAAGGAAGCCATACTCCAAGAGCTCAAAAAATCGTTGAGTGACAAAAGATATTTTCTTGTGCTCGATGATGTTTGGAATGAGAATCCGAACGTGTGGAATGAGCTAAAGGGTTGTTTGTTAAAAGTTAATAAGAACTTTGGGAGTGTGATTGTTGTCACTACTAGGAGCGATGAAGTTGCGAAAATCGTGGAGACACAATGTTGTAGGCATGATTTGAGAAAGTTATCAAATGATCAGTGTTGGTCTTTGTTTGAGAAATGTATATTTGCTAATGAATTGCCAAAAACTTTGAAGATGGATAAGGTTCGAGAGGAGCTTATTGAACAATTTGGTGGCATGCCACTAGTTGTGAAAGCATTGGGAGGAATGGTGAAATTGGACAAGAATCATGAAGAATTGGAGTCCACGTTGAAAAATCTAATTAGAATTCCTTCCAATGAAAATCACGCTTTATCTACGATGAAGTTAAGTGTGGATCGCTTACCATCATCTTCGTTAAAACAATCTTTCGCCTATCGTTCAAAATTTTCTCAAGATTTCAAGTTCAAGAAAGAATAG

Coding sequence (CDS)

ATGGCGGAATTCCTGTGGAGTTTTGCTGTTCAGGAAGTCTTGAAGAAGATAGTGAAGCTGGCGGCCGAGCAGATCGGTTTGGCATGGGGTTTCAAGAAGGAGCTCTCAAAGCTCAAGGACTCTCTACTCATGGTGGAAGCCATCCTTCACGACGTCCACAAAATCAAGCTAGACCATCAAGCTGTGAAGCTATGGGTGGAGAAGCTCGAAGATGTCGTCTTCGAAGCCGATGTCTTGCTCGACAAGCTCGCTTACGACGATCTCCGACGCAAGGTCGTCGGAACCGGAAAAGAGGCCATGGTAAGTTACTTCGTTTCATCTTCCAAAAACCCCCTTGTTTTTCGCCTCAAAATGGCCAATAGAATCAAGGCCGTTGCCAAAGCCTTACACGAGCATTACCGTGCAGCAAGTGTTGTGGGGCTCGTTGCTTTGACATCCAAAGATGTTGAAACTGAACTCAGCCAGATTCAAGAGACAGACTCCTTTTTGGATGAGATTGGAGTCATTGGAAGAGAAATTGAAGTATGTGAGATTGTGAACAAGCTCATTGACCTCAGTAGTCAAGAAGCTTTGTCTGTTCTACCCATTGTTGGTGAGGGTGGACTAGGGAAAACAGCTTTGGCAAAGGTAATCTTCAATCATGAGGTGATAAGGGAGAATTTTGATACAACTATATGGGTGTATGTGTCTAGACATTTTGTCATCAAGAAGATCTTGAAAGCTATTTTGGAAACTCTCAATGCCAATTTTGGTGGGTTGGATAGCAAGGAAGCCATACTCCAAGAGCTCAAAAAATCGTTGAGTGACAAAAGATATTTTCTTGTGCTCGATGATGTTTGGAATGAGAATCCGAACGTGTGGAATGAGCTAAAGGGTTGTTTGTTAAAAGTTAATAAGAACTTTGGGAGTGTGATTGTTGTCACTACTAGGAGCGATGAAGTTGCGAAAATCGTGGAGACACAATGTTGTAGGCATGATTTGAGAAAGTTATCAAATGATCAGTGTTGGTCTTTGTTTGAGAAATGTATATTTGCTAATGAATTGCCAAAAACTTTGAAGATGGATAAGGTTCGAGAGGAGCTTATTGAACAATTTGGTGGCATGCCACTAGTTGTGAAAGCATTGGGAGGAATGGTGAAATTGGACAAGAATCATGAAGAATTGGAGTCCACGTTGAAAAATCTAATTAGAATTCCTTCCAATGAAAATCACGCTTTATCTACGATGAAGTTAAGTGTGGATCGCTTACCATCATCTTCGTTAAAACAATCTTTCGCCTATCGTTCAAAATTTTCTCAAGATTTCAAGTTCAAGAAAGAATAG

Protein sequence

MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIPSNENHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFKFKKE
Homology
BLAST of Spg005307 vs. NCBI nr
Match: XP_022145226.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 641.3 bits (1653), Expect = 5.9e-180
Identity = 341/444 (76.80%), Postives = 388/444 (87.39%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAE +WSFAVQEVLKKIVKLAAEQ+ LAWGFKKEL+KLKDSLLM+E ILHD+ +IK +H+
Sbjct: 1   MAELVWSFAVQEVLKKIVKLAAEQMSLAWGFKKELAKLKDSLLMMETILHDLDRIKAEHK 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           AVKLWVEKLEDVVFEADVLLDK+A++DLRRK V TGKE MVSYFVSSSKNPLVFRLKMA+
Sbjct: 61  AVKLWVEKLEDVVFEADVLLDKIAFEDLRRK-VETGKEVMVSYFVSSSKNPLVFRLKMAS 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVET--ELSQIQETDSFLDEIGVIGREIEVCEI 180
           +IK +AKALHEHYRAAS VGLVA+TSK+ E+  +++QI ETDSFLDEIGVIGREIEVCEI
Sbjct: 121 KIKGIAKALHEHYRAASAVGLVAITSKETESDDDITQILETDSFLDEIGVIGREIEVCEI 180

Query: 181 VNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKI 240
           VNKLID S  EAL VLPIVG GGLGKTA+AKVIFNHEVIRENFDTT+WVYVS+ FVIKKI
Sbjct: 181 VNKLIDPSHHEALFVLPIVGLGGLGKTAVAKVIFNHEVIRENFDTTLWVYVSKSFVIKKI 240

Query: 241 LKAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVN 300
           L+AILETLNAN  G DSKEA+LQELKK L+DKRYFLVLDDVWNEN N+WNELK CLLK+ 
Sbjct: 241 LRAILETLNANSCGFDSKEALLQELKKWLNDKRYFLVLDDVWNENLNLWNELKACLLKIT 300

Query: 301 KNFGSVIVVTTRSDEVAKIVETQ-CCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKV 360
           K  G V+VVTTRSDEVA+I ETQ   RH LR+LS+D CWSLFEKC F NE P TLK+D+V
Sbjct: 301 KKSGCVVVVTTRSDEVAEITETQYYSRHYLRRLSDDHCWSLFEKCAFINEWPTTLKLDEV 360

Query: 361 REELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIPSNENHALSTMKLSVDRLP 420
           REEL+E+F G+PLVVKALGGMVK+DKN + L+ TL+NLIRI   + + +ST+KL VDRLP
Sbjct: 361 REELVEKFSGIPLVVKALGGMVKIDKNRDGLQCTLENLIRILLQDENYISTIKLGVDRLP 420

Query: 421 -SSSLKQSFAYRSKFSQDFKFKKE 441
            S SLKQ  +  SKFSQDFKFK+E
Sbjct: 421 LSFSLKQYNS--SKFSQDFKFKRE 441

BLAST of Spg005307 vs. NCBI nr
Match: XP_004152291.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KGN53095.1 hypothetical protein Csa_015377 [Cucumis sativus])

HSP 1 Score: 600.5 bits (1547), Expect = 1.2e-167
Identity = 325/445 (73.03%), Postives = 374/445 (84.04%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MA+FLWSFAV EVLKK VKL AEQIG++WGFKK+LSKL+DSLLMVEAIL DV++IK +HQ
Sbjct: 1   MADFLWSFAVDEVLKKTVKLVAEQIGMSWGFKKDLSKLRDSLLMVEAILRDVNRIKAEHQ 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           A++LWVEKLE +VFEADVLLD+L+Y+DLRRKV        V  FVSSSKNPLVFRLKMAN
Sbjct: 61  ALRLWVEKLEHIVFEADVLLDELSYEDLRRKV----DARPVRSFVSSSKNPLVFRLKMAN 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVN 180
           +IKA+AK L EHY AAS++GLVA+TSK+VE+E SQI ETDSFLDEIGVIGRE EV EIVN
Sbjct: 121 KIKAIAKRLDEHYCAASIMGLVAITSKEVESEPSQILETDSFLDEIGVIGREAEVLEIVN 180

Query: 181 KLIDLSSQE-ALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKIL 240
           KL++LS QE ALSVLPIVG GGLGKT+LAK IF+HE+IRENFD  IWV VS  FVI KIL
Sbjct: 181 KLLELSKQEAALSVLPIVGIGGLGKTSLAKAIFHHEMIRENFDRMIWVCVSEPFVINKIL 240

Query: 241 KAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNK 300
           +AILETLNANFGGLD+KEA+LQEL+K L +K+YFLVLDDVWNENP++WNEL+ CLLK NK
Sbjct: 241 RAILETLNANFGGLDNKEALLQELQKLLRNKKYFLVLDDVWNENPDLWNELRACLLKANK 300

Query: 301 NFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKV-R 360
            FGSVIVVTTRSDEVA IVET   RH LRKLSND CW+LFEKC F ++LP T ++D V R
Sbjct: 301 KFGSVIVVTTRSDEVANIVETNHQRHRLRKLSNDYCWTLFEKCAFGSDLPVTPRVDHVIR 360

Query: 361 EELIEQFGGMPLVVKALGGMVKLDKNH--EELESTLKNLIRIP-SNENHALSTMKLSVDR 420
           EEL+++FGG+PLVVK  GGMVKLDKN   + L STL+NLI  P   EN  LST+KLSVDR
Sbjct: 361 EELVKRFGGIPLVVKVFGGMVKLDKNKCCQGLRSTLENLIISPLQYENSILSTIKLSVDR 420

Query: 421 LPSSSLKQSFAYRSKFSQDFKFKKE 441
           LPSSSLKQ FAY S F + F F +E
Sbjct: 421 LPSSSLKQCFAYCSNFPRGFLFIRE 441

BLAST of Spg005307 vs. NCBI nr
Match: XP_038874765.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 594.0 bits (1530), Expect = 1.1e-165
Identity = 312/443 (70.43%), Postives = 369/443 (83.30%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           M EFLW+FAVQE+LKK VKLAAEQIGL WGFKK+LSK++DSLLMV+AIL DV +IK ++ 
Sbjct: 1   MVEFLWTFAVQEILKKTVKLAAEQIGLGWGFKKDLSKMRDSLLMVQAILRDVDRIKSEYP 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKV-VGTGKEAMVSYFVSSSKNPLVFRLKMA 120
            VK WVEKLED+VFEADVLLD+LAY+DLR KV +G      V  F+S+S+NPL FRLKMA
Sbjct: 61  DVKPWVEKLEDIVFEADVLLDELAYEDLRLKVEIGKDLGKKVHNFISASENPLFFRLKMA 120

Query: 121 NRIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIV 180
           NRIKA+++ L EHY AASVVGLVA+TSK++E +LSQ+ ETDSFLDE GVIGR+ EV EIV
Sbjct: 121 NRIKAISRMLQEHYAAASVVGLVAITSKEIEPDLSQVHETDSFLDEFGVIGRDEEVLEIV 180

Query: 181 NKLIDLSSQE-ALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKI 240
           NKLI+LS+ E  LSVLPIVG GGLGKTALAKVIFNHEVI+ENFD  IWV VS  FVI KI
Sbjct: 181 NKLIELSNGEMPLSVLPIVGMGGLGKTALAKVIFNHEVIKENFDRFIWVCVSDPFVINKI 240

Query: 241 LKAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVN 300
           L+AILETLN N GGL+SKEA+LQE++K L DK YFLVLDDVWNEN ++WNELK CLLKV 
Sbjct: 241 LRAILETLNPNSGGLESKEALLQEIQKLLHDKNYFLVLDDVWNENLSLWNELKACLLKVT 300

Query: 301 KNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVR 360
           + FG+V++VT+RSDEVAKI+ET   RH L+KLSND CW LFEKC F ++LP   K+DK+R
Sbjct: 301 RKFGNVVIVTSRSDEVAKIMETH-PRHYLKKLSNDHCWLLFEKCAFGSDLPTIPKLDKIR 360

Query: 361 EELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIP-SNENHALSTMKLSVDRLP 420
            EL+E FGG+PLVVK LGG VKLDKN+E L+S L NL+RIP  +EN  LST+KLSVDRLP
Sbjct: 361 RELVENFGGIPLVVKVLGGTVKLDKNYERLQSPLTNLMRIPLQDENRVLSTIKLSVDRLP 420

Query: 421 SSSLKQSFAYRSKFSQDFKFKKE 441
           S SLKQ FAY S F +DF+F+K+
Sbjct: 421 SPSLKQCFAYCSNFPRDFEFRKD 442

BLAST of Spg005307 vs. NCBI nr
Match: XP_022145225.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 591.3 bits (1523), Expect = 7.0e-165
Identity = 314/444 (70.72%), Postives = 371/444 (83.56%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKL--D 60
           MAEFLW+FAVQEVLKK V+LAAEQIG+AWGF KELSKL+DSL+MVEAIL DV +IK   +
Sbjct: 41  MAEFLWTFAVQEVLKKTVRLAAEQIGMAWGFNKELSKLRDSLVMVEAILRDVDRIKAEPE 100

Query: 61  HQAVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVS-YFVSSSKNPLVFRLK 120
           H A+KLWVEKL+D+VFEADVLLD +AY+DLRRKV     + MVS  F SSSKNP++FRLK
Sbjct: 101 HHALKLWVEKLQDIVFEADVLLDDIAYEDLRRKVETGKVQVMVSNCFASSSKNPIIFRLK 160

Query: 121 MANRIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCE 180
           MA +IK +AK L  HY  AS VGLVA TSK+ E +LSQIQETDSFLDEIGVIGRE EV E
Sbjct: 161 MARKIKVIAKMLDRHYYRASAVGLVARTSKETEPDLSQIQETDSFLDEIGVIGRESEVSE 220

Query: 181 IVNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKK 240
           IVNKLI+LS+QEALS+LPIVG  GLGKT+LAKVIFNH +I+ NFD  IWV VS  FVI K
Sbjct: 221 IVNKLIELSNQEALSILPIVGMPGLGKTSLAKVIFNHGLIKANFDEIIWVCVSEPFVINK 280

Query: 241 ILKAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKV 300
           IL+++LETLN N GGL+SKEAILQEL+K L+ K+YFLV DDVWNENP++W+EL+ CLLK+
Sbjct: 281 ILRSVLETLNPNSGGLESKEAILQELQKLLNAKKYFLVFDDVWNENPDLWSELRACLLKI 340

Query: 301 NKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKV 360
            +  G+V+VVTTRS+EVAKI+ET   RH LRKLSN+QCWSLFEKC F NELP   KMD V
Sbjct: 341 TQESGNVLVVTTRSEEVAKIMETH-HRHHLRKLSNNQCWSLFEKCAFGNELPIIPKMDVV 400

Query: 361 REELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIP-SNENHALSTMKLSVDRL 420
           +EELI++FGG+PLVVK LGG+VKL+KN E L+STL+N++RIP  +++H LST+KLSVDRL
Sbjct: 401 KEELIKKFGGIPLVVKVLGGIVKLEKNPEGLQSTLENIMRIPLQDKSHVLSTIKLSVDRL 460

Query: 421 PSSSLKQSFAYRSKFSQDFKFKKE 441
           PS SLKQ FAY SKF  DF F KE
Sbjct: 461 PSPSLKQCFAYCSKFPPDFLFVKE 483

BLAST of Spg005307 vs. NCBI nr
Match: XP_008453966.1 (PREDICTED: putative disease resistance protein RGA3 [Cucumis melo] >TYK16977.1 putative disease resistance protein RGA3 [Cucumis melo var. makuwa])

HSP 1 Score: 587.8 bits (1514), Expect = 7.8e-164
Identity = 307/441 (69.61%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAEFLW+FA QE+LKK VKLAAEQIGLAWGF  ELS L+DSLLMVEAIL DV +IK +HQ
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           AVKLWVEKLE +VFE DVLLD+LAY+DLRRK V + KEA+VS F+S SK PLVFRLKMAN
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRK-VESQKEAIVSNFISFSKTPLVFRLKMAN 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVN 180
           +IK +AK L +HY AAS VGLVA+ SK  E + SQIQETDSFLDE GVIGRE EV EIVN
Sbjct: 121 KIKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVN 180

Query: 181 KLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILK 240
             +DLS +E LSVLPIVG GGLGKTALAKVIFNHE+I+ NFD T+WV VS  F+IKKIL+
Sbjct: 181 VSVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILR 240

Query: 241 AILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKN 300
           AILETLN+NFGGLDSKEA+LQEL+K L+DK+YFLVLDDVWNENP +WNELKGCLLK+++ 
Sbjct: 241 AILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQR 300

Query: 301 FGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREE 360
            G+V+VVTTRSD VA+I+ET   R+ L KLS+D CWSLF+K  F NEL    ++D V++E
Sbjct: 301 SGNVVVVTTRSDRVAEIMETH-SRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKE 360

Query: 361 LIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIP-SNENHALSTMKLSVDRLPSS 420
           L+++FGG+PL VK +GG+VK D+NHE L+ +L+NL+R+   +ENH +ST+KL+VDRLP S
Sbjct: 361 LVKRFGGIPLAVKVMGGIVKFDENHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLS 420

Query: 421 SLKQSFAYRSKFSQDFKFKKE 441
           SLKQ FAY S F +DFKF++E
Sbjct: 421 SLKQCFAYCSNFPKDFKFRRE 439

BLAST of Spg005307 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 1.2e-55
Identity = 146/436 (33.49%), Postives = 247/436 (56.65%), Query Frame = 0

Query: 10  VQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKL 69
           +Q +L  +      ++GL +GF+KE  KL     M++A+L D  + +L ++A+K W++KL
Sbjct: 6   LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKL 65

Query: 70  EDVVFEADVLLDKLAYDDLRRK--VVGTGKEAMVSYFVSSSKNPLVFRLK-MANRIKAVA 129
               +E D +LD    +  R K  V+G      +++     K     R+K M  ++ A+A
Sbjct: 66  NVAAYEVDDILDDCKTEAARFKQAVLGRYHPRTITFCYKVGK-----RMKEMMEKLDAIA 125

Query: 130 KALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLI-DL 189
           +     +          L  + +E + ++ ++T   L E  V GRE E  EIV  LI ++
Sbjct: 126 EERRNFH----------LDERIIERQAAR-RQTGFVLTEPKVYGREKEEDEIVKILINNV 185

Query: 190 SSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILET 249
           S  E + VLPI+G GGLGKT LA+++FN + I E+F+  IWV VS  F  K+++KAI+E+
Sbjct: 186 SYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVES 245

Query: 250 LNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVI 309
           +     G      + ++L++ L+ KRYFLVLDDVWNE+   W+ L+  +LK+  + G+ I
Sbjct: 246 IEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLR-AVLKIGAS-GASI 305

Query: 310 VVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREELIEQF 369
           ++TTR +++  I+ T    + L  LS + CW LF++  F ++   + K+ ++ +E++++ 
Sbjct: 306 LITTRLEKIGSIMGT-LQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKC 365

Query: 370 GGMPLVVKALGGMVKLDKNHEELESTLKNLI-RIPSNENHALSTMKLSVDRLPSSSLKQS 429
           GG+PL  K LGG+++  +   E E    + I  +P +EN  L  ++LS   LP   L+Q 
Sbjct: 366 GGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLP-LDLRQC 421

Query: 430 FAYRSKFSQDFKFKKE 441
           FAY + F +D K +KE
Sbjct: 426 FAYCAVFPKDTKIEKE 421

BLAST of Spg005307 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 7.3e-48
Identity = 137/433 (31.64%), Postives = 231/433 (53.35%), Query Frame = 0

Query: 10  VQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKL 69
           +Q VL  +      ++ L +GF+ E  +L      ++A+L D  + +L+ + ++ W++KL
Sbjct: 6   IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65

Query: 70  EDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAKAL 129
               +E D +LD+      R          + S +       + FR K+  R+  V K L
Sbjct: 66  NAATYEVDDILDEYKTKATR---------FLQSEYGRYHPKVIPFRHKVGKRMDQVMKKL 125

Query: 130 HEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLIDLSSQ- 189
           +     A       L  K +E + +  +ET S L E  V GR+ E  EIV  LI+ +S  
Sbjct: 126 N---AIAEERKKFHLQEKIIERQ-AATRETGSVLTEPQVYGRDKEKDEIVKILINTASDA 185

Query: 190 EALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILETLNA 249
           + LSVLPI+G GGLGKT L++++FN + + E F   IW+ +S  F  K+++KAI+E++  
Sbjct: 186 QKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEG 245

Query: 250 NFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVIVVT 309
                     + ++L++ L+ KRYFLVLDDVWNE+ + W  L+  +LKV  + G+ ++ T
Sbjct: 246 KSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLR-AVLKVGAS-GAFVLTT 305

Query: 310 TRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREELIEQFGGM 369
           TR ++V  I+ T    ++L  LS + CW LF +  F ++      +  + +E++++ GG+
Sbjct: 306 TRLEKVGSIMGT-LQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGV 365

Query: 370 PLVVKALGGMVKLDKNHEELESTLKNLI-RIPSNENHALSTMKLSVDRLPSSSLKQSFAY 429
           PL  K LGG+++  +   E E    + I  +P +E+  L  ++LS   LP   L+Q F Y
Sbjct: 366 PLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLP-LDLRQCFVY 421

Query: 430 RSKFSQDFKFKKE 441
            + F +D K  KE
Sbjct: 426 CAVFPKDTKMAKE 421

BLAST of Spg005307 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.2e-47
Identity = 141/435 (32.41%), Postives = 235/435 (54.02%), Query Frame = 0

Query: 10  VQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKL 69
           +Q +L  +      ++ L +GF+ E  +L      ++A+L D  + +L+++ ++ W++KL
Sbjct: 6   IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65

Query: 70  EDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAKAL 129
               +E D +L     D+ + K     +     Y        + FR K+  R+  V K L
Sbjct: 66  NAATYEVDDIL-----DEYKTKATRFSQSEYGRY----HPKVIPFRHKVGKRMDQVMKKL 125

Query: 130 HEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLI-DLSSQ 189
                 A       L  K VE +  + +ET S L E  V GR+ E  EIV  LI ++S  
Sbjct: 126 K---AIAEERKNFHLHEKIVERQAVR-RETGSVLTEPQVYGRDKEKDEIVKILINNVSDA 185

Query: 190 EALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILETLNA 249
           + LSVLPI+G GGLGKT LA+++FN + + E+F + IW+ VS  F  K+++KAI+E++  
Sbjct: 186 QHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEG 245

Query: 250 N--FGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVIV 309
               G +D    + ++L++ L+ KRY LVLDDVWNE+   W  L+  +LKV  + G+ ++
Sbjct: 246 RPLLGEMD-LAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLR-AVLKVGAS-GASVL 305

Query: 310 VTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREELIEQFG 369
            TTR ++V  I+ T    ++L  LS + CW LF +  F ++      +  + +E++++ G
Sbjct: 306 TTTRLEKVGSIMGT-LQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSG 365

Query: 370 GMPLVVKALGGMVKLDKNHEELESTLKNLI-RIPSNENHALSTMKLSVDRLPSSSLKQSF 429
           G+PL  K LGG++   +     E    + I  +P +E+  L  ++LS  +LP   LKQ F
Sbjct: 366 GVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLP-LDLKQCF 422

Query: 430 AYRSKFSQDFKFKKE 441
           AY + F +D K +KE
Sbjct: 426 AYCAVFPKDAKMEKE 422

BLAST of Spg005307 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 134/436 (30.73%), Postives = 230/436 (52.75%), Query Frame = 0

Query: 10  VQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKL 69
           +Q +L+ +     +++ L +GF+KE  KL      ++A+L D  + +L  +A++ W++KL
Sbjct: 6   LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65

Query: 70  EDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAK-- 129
               +E D +L +   + +R       +++ + ++     N   FR K+  R+K + +  
Sbjct: 66  NSAAYEVDDILGECKNEAIR------FEQSRLGFYHPGIIN---FRHKIGRRMKEIMEKL 125

Query: 130 -ALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLI-DL 189
            A+ E  R      L  +T +      +  +ET   L E  V GR+ E  EIV  LI ++
Sbjct: 126 DAISEERRKFHF--LEKITERQA---AAATRETGFVLTEPKVYGRDKEEDEIVKILINNV 185

Query: 190 SSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILET 249
           +  E L V PI+G GGLGKT LA++IFN E + ++F+  IWV VS  F  K+++K I+  
Sbjct: 186 NVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGN 245

Query: 250 LNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVI 309
           +  +   ++   +  ++L++ L+ KRY LVLDDVWN++   W +L+  L    +  G+ I
Sbjct: 246 IERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGAR--GASI 305

Query: 310 VVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREELIEQF 369
           + TTR ++V  I+ T    H L  LS      LF +  F  +      +  + +E++++ 
Sbjct: 306 LATTRLEKVGSIMGTLQPYH-LSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKC 365

Query: 370 GGMPLVVKALGGMVKLDKNHEELESTLKNLI-RIPSNENHALSTMKLSVDRLPSSSLKQS 429
           GG+PL  K LGG+++  +   E E    N I  +P +E+  L  ++LS   LP   L+Q 
Sbjct: 366 GGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHLP-LDLRQC 423

Query: 430 FAYRSKFSQDFKFKKE 441
           FAY + F +D K  KE
Sbjct: 426 FAYCAVFPKDTKMIKE 423

BLAST of Spg005307 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 6.0e-42
Identity = 128/422 (30.33%), Postives = 227/422 (53.79%), Query Frame = 0

Query: 35  LSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVG 94
           L +L  +LL + A+L D  + ++ +  V+ WV +L DVV+ A+  LD +A + LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95  -----------TGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAKALHEHYRAASVVGLVA 154
                       G+ ++  +   +S++       +  R++ V   L       +++GL  
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEH-------LETRLEKVTIRLERLASQRNILGLKE 158

Query: 155 LTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLIDLSSQE-ALSVLPIVGEGGL 214
           LT+   +  L     T S +DE  V GR+ +  EI+  LI  + ++  ++V+ IVG GG+
Sbjct: 159 LTAMIPKQRL----PTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 218

Query: 215 GKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILETLNANFGGLDSKEAILQE 274
           GKT L+++++N + +R  F T +W +VS  F + KI K + E++ +        + +  +
Sbjct: 219 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 278

Query: 275 LKKSLSDK--RYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVIVVTTRSDEVAKIVET 334
           LK+ L+     + LVLDD+WNEN   W+ L+   +   +  GS I+VTTRS  VA I+  
Sbjct: 279 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQ--GSQILVTTRSQRVASIM-C 338

Query: 335 QCCRHDLRKLSNDQCWSLFEKCIFANELP-KTLKMDKVREELIEQFGGMPLVVKALGGMV 394
               H+L+ LS+  CWSLF K +F N+ P    ++  + E ++ +  G+PL VK LGG++
Sbjct: 339 AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 398

Query: 395 KLDKNHEELESTLKNLI-RIPSNENHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFKFK 441
           + +    E E  L + I  +P+++++ L  +++S   LP + LK+ FAY S F +   F+
Sbjct: 399 RFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 445

BLAST of Spg005307 vs. ExPASy TrEMBL
Match: A0A6J1CVQ5 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014730 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.9e-180
Identity = 341/444 (76.80%), Postives = 388/444 (87.39%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAE +WSFAVQEVLKKIVKLAAEQ+ LAWGFKKEL+KLKDSLLM+E ILHD+ +IK +H+
Sbjct: 1   MAELVWSFAVQEVLKKIVKLAAEQMSLAWGFKKELAKLKDSLLMMETILHDLDRIKAEHK 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           AVKLWVEKLEDVVFEADVLLDK+A++DLRRK V TGKE MVSYFVSSSKNPLVFRLKMA+
Sbjct: 61  AVKLWVEKLEDVVFEADVLLDKIAFEDLRRK-VETGKEVMVSYFVSSSKNPLVFRLKMAS 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVET--ELSQIQETDSFLDEIGVIGREIEVCEI 180
           +IK +AKALHEHYRAAS VGLVA+TSK+ E+  +++QI ETDSFLDEIGVIGREIEVCEI
Sbjct: 121 KIKGIAKALHEHYRAASAVGLVAITSKETESDDDITQILETDSFLDEIGVIGREIEVCEI 180

Query: 181 VNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKI 240
           VNKLID S  EAL VLPIVG GGLGKTA+AKVIFNHEVIRENFDTT+WVYVS+ FVIKKI
Sbjct: 181 VNKLIDPSHHEALFVLPIVGLGGLGKTAVAKVIFNHEVIRENFDTTLWVYVSKSFVIKKI 240

Query: 241 LKAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVN 300
           L+AILETLNAN  G DSKEA+LQELKK L+DKRYFLVLDDVWNEN N+WNELK CLLK+ 
Sbjct: 241 LRAILETLNANSCGFDSKEALLQELKKWLNDKRYFLVLDDVWNENLNLWNELKACLLKIT 300

Query: 301 KNFGSVIVVTTRSDEVAKIVETQ-CCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKV 360
           K  G V+VVTTRSDEVA+I ETQ   RH LR+LS+D CWSLFEKC F NE P TLK+D+V
Sbjct: 301 KKSGCVVVVTTRSDEVAEITETQYYSRHYLRRLSDDHCWSLFEKCAFINEWPTTLKLDEV 360

Query: 361 REELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIPSNENHALSTMKLSVDRLP 420
           REEL+E+F G+PLVVKALGGMVK+DKN + L+ TL+NLIRI   + + +ST+KL VDRLP
Sbjct: 361 REELVEKFSGIPLVVKALGGMVKIDKNRDGLQCTLENLIRILLQDENYISTIKLGVDRLP 420

Query: 421 -SSSLKQSFAYRSKFSQDFKFKKE 441
            S SLKQ  +  SKFSQDFKFK+E
Sbjct: 421 LSFSLKQYNS--SKFSQDFKFKRE 441

BLAST of Spg005307 vs. ExPASy TrEMBL
Match: A0A0A0KTZ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015850 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 5.6e-168
Identity = 325/445 (73.03%), Postives = 374/445 (84.04%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MA+FLWSFAV EVLKK VKL AEQIG++WGFKK+LSKL+DSLLMVEAIL DV++IK +HQ
Sbjct: 1   MADFLWSFAVDEVLKKTVKLVAEQIGMSWGFKKDLSKLRDSLLMVEAILRDVNRIKAEHQ 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           A++LWVEKLE +VFEADVLLD+L+Y+DLRRKV        V  FVSSSKNPLVFRLKMAN
Sbjct: 61  ALRLWVEKLEHIVFEADVLLDELSYEDLRRKV----DARPVRSFVSSSKNPLVFRLKMAN 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVN 180
           +IKA+AK L EHY AAS++GLVA+TSK+VE+E SQI ETDSFLDEIGVIGRE EV EIVN
Sbjct: 121 KIKAIAKRLDEHYCAASIMGLVAITSKEVESEPSQILETDSFLDEIGVIGREAEVLEIVN 180

Query: 181 KLIDLSSQE-ALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKIL 240
           KL++LS QE ALSVLPIVG GGLGKT+LAK IF+HE+IRENFD  IWV VS  FVI KIL
Sbjct: 181 KLLELSKQEAALSVLPIVGIGGLGKTSLAKAIFHHEMIRENFDRMIWVCVSEPFVINKIL 240

Query: 241 KAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNK 300
           +AILETLNANFGGLD+KEA+LQEL+K L +K+YFLVLDDVWNENP++WNEL+ CLLK NK
Sbjct: 241 RAILETLNANFGGLDNKEALLQELQKLLRNKKYFLVLDDVWNENPDLWNELRACLLKANK 300

Query: 301 NFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKV-R 360
            FGSVIVVTTRSDEVA IVET   RH LRKLSND CW+LFEKC F ++LP T ++D V R
Sbjct: 301 KFGSVIVVTTRSDEVANIVETNHQRHRLRKLSNDYCWTLFEKCAFGSDLPVTPRVDHVIR 360

Query: 361 EELIEQFGGMPLVVKALGGMVKLDKNH--EELESTLKNLIRIP-SNENHALSTMKLSVDR 420
           EEL+++FGG+PLVVK  GGMVKLDKN   + L STL+NLI  P   EN  LST+KLSVDR
Sbjct: 361 EELVKRFGGIPLVVKVFGGMVKLDKNKCCQGLRSTLENLIISPLQYENSILSTIKLSVDR 420

Query: 421 LPSSSLKQSFAYRSKFSQDFKFKKE 441
           LPSSSLKQ FAY S F + F F +E
Sbjct: 421 LPSSSLKQCFAYCSNFPRGFLFIRE 441

BLAST of Spg005307 vs. ExPASy TrEMBL
Match: A0A6J1CTV3 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014729 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 3.4e-165
Identity = 314/444 (70.72%), Postives = 371/444 (83.56%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKL--D 60
           MAEFLW+FAVQEVLKK V+LAAEQIG+AWGF KELSKL+DSL+MVEAIL DV +IK   +
Sbjct: 41  MAEFLWTFAVQEVLKKTVRLAAEQIGMAWGFNKELSKLRDSLVMVEAILRDVDRIKAEPE 100

Query: 61  HQAVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVS-YFVSSSKNPLVFRLK 120
           H A+KLWVEKL+D+VFEADVLLD +AY+DLRRKV     + MVS  F SSSKNP++FRLK
Sbjct: 101 HHALKLWVEKLQDIVFEADVLLDDIAYEDLRRKVETGKVQVMVSNCFASSSKNPIIFRLK 160

Query: 121 MANRIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCE 180
           MA +IK +AK L  HY  AS VGLVA TSK+ E +LSQIQETDSFLDEIGVIGRE EV E
Sbjct: 161 MARKIKVIAKMLDRHYYRASAVGLVARTSKETEPDLSQIQETDSFLDEIGVIGRESEVSE 220

Query: 181 IVNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKK 240
           IVNKLI+LS+QEALS+LPIVG  GLGKT+LAKVIFNH +I+ NFD  IWV VS  FVI K
Sbjct: 221 IVNKLIELSNQEALSILPIVGMPGLGKTSLAKVIFNHGLIKANFDEIIWVCVSEPFVINK 280

Query: 241 ILKAILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKV 300
           IL+++LETLN N GGL+SKEAILQEL+K L+ K+YFLV DDVWNENP++W+EL+ CLLK+
Sbjct: 281 ILRSVLETLNPNSGGLESKEAILQELQKLLNAKKYFLVFDDVWNENPDLWSELRACLLKI 340

Query: 301 NKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKV 360
            +  G+V+VVTTRS+EVAKI+ET   RH LRKLSN+QCWSLFEKC F NELP   KMD V
Sbjct: 341 TQESGNVLVVTTRSEEVAKIMETH-HRHHLRKLSNNQCWSLFEKCAFGNELPIIPKMDVV 400

Query: 361 REELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIP-SNENHALSTMKLSVDRL 420
           +EELI++FGG+PLVVK LGG+VKL+KN E L+STL+N++RIP  +++H LST+KLSVDRL
Sbjct: 401 KEELIKKFGGIPLVVKVLGGIVKLEKNPEGLQSTLENIMRIPLQDKSHVLSTIKLSVDRL 460

Query: 421 PSSSLKQSFAYRSKFSQDFKFKKE 441
           PS SLKQ FAY SKF  DF F KE
Sbjct: 461 PSPSLKQCFAYCSKFPPDFLFVKE 483

BLAST of Spg005307 vs. ExPASy TrEMBL
Match: A0A5D3CZ17 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001180 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.8e-164
Identity = 307/441 (69.61%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAEFLW+FA QE+LKK VKLAAEQIGLAWGF  ELS L+DSLLMVEAIL DV +IK +HQ
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           AVKLWVEKLE +VFE DVLLD+LAY+DLRRK V + KEA+VS F+S SK PLVFRLKMAN
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRK-VESQKEAIVSNFISFSKTPLVFRLKMAN 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVN 180
           +IK +AK L +HY AAS VGLVA+ SK  E + SQIQETDSFLDE GVIGRE EV EIVN
Sbjct: 121 KIKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVN 180

Query: 181 KLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILK 240
             +DLS +E LSVLPIVG GGLGKTALAKVIFNHE+I+ NFD T+WV VS  F+IKKIL+
Sbjct: 181 VSVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILR 240

Query: 241 AILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKN 300
           AILETLN+NFGGLDSKEA+LQEL+K L+DK+YFLVLDDVWNENP +WNELKGCLLK+++ 
Sbjct: 241 AILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQR 300

Query: 301 FGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREE 360
            G+V+VVTTRSD VA+I+ET   R+ L KLS+D CWSLF+K  F NEL    ++D V++E
Sbjct: 301 SGNVVVVTTRSDRVAEIMETH-SRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKE 360

Query: 361 LIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIP-SNENHALSTMKLSVDRLPSS 420
           L+++FGG+PL VK +GG+VK D+NHE L+ +L+NL+R+   +ENH +ST+KL+VDRLP S
Sbjct: 361 LVKRFGGIPLAVKVMGGIVKFDENHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLS 420

Query: 421 SLKQSFAYRSKFSQDFKFKKE 441
           SLKQ FAY S F +DFKF++E
Sbjct: 421 SLKQCFAYCSNFPKDFKFRRE 439

BLAST of Spg005307 vs. ExPASy TrEMBL
Match: A0A5A7TSS6 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003620 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.8e-164
Identity = 307/441 (69.61%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAEFLW+FA QE+LKK VKLAAEQIGLAWGF  ELS L+DSLLMVEAIL DV +IK +HQ
Sbjct: 1   MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
           AVKLWVEKLE +VFE DVLLD+LAY+DLRRK V + KEA+VS F+S SK PLVFRLKMAN
Sbjct: 61  AVKLWVEKLEAIVFEVDVLLDELAYEDLRRK-VESQKEAIVSNFISFSKTPLVFRLKMAN 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVN 180
           +IK +AK L +HY AAS VGLVA+ SK  E + SQIQETDSFLDE GVIGRE EV EIVN
Sbjct: 121 KIKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVN 180

Query: 181 KLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILK 240
             +DLS +E LSVLPIVG GGLGKTALAKVIFNHE+I+ NFD T+WV VS  F+IKKIL+
Sbjct: 181 VSVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILR 240

Query: 241 AILETLNANFGGLDSKEAILQELKKSLSDKRYFLVLDDVWNENPNVWNELKGCLLKVNKN 300
           AILETLN+NFGGLDSKEA+LQEL+K L+DK+YFLVLDDVWNENP +WNELKGCLLK+++ 
Sbjct: 241 AILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQR 300

Query: 301 FGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLKMDKVREE 360
            G+V+VVTTRSD VA+I+ET   R+ L KLS+D CWSLF+K  F NEL    ++D V++E
Sbjct: 301 SGNVVVVTTRSDRVAEIMETH-SRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKE 360

Query: 361 LIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNLIRIP-SNENHALSTMKLSVDRLPSS 420
           L+++FGG+PL VK +GG+VK D+NHE L+ +L+NL+R+   +ENH +ST+KL+VDRLP S
Sbjct: 361 LVKRFGGIPLAVKVMGGIVKFDENHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLS 420

Query: 421 SLKQSFAYRSKFSQDFKFKKE 441
           SLKQ FAY S F +DFKF++E
Sbjct: 421 SLKQCFAYCSNFPKDFKFRRE 439

BLAST of Spg005307 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 173.3 bits (438), Expect = 4.2e-43
Identity = 128/422 (30.33%), Postives = 227/422 (53.79%), Query Frame = 0

Query: 35  LSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVG 94
           L +L  +LL + A+L D  + ++ +  V+ WV +L DVV+ A+  LD +A + LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95  -----------TGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAKALHEHYRAASVVGLVA 154
                       G+ ++  +   +S++       +  R++ V   L       +++GL  
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEH-------LETRLEKVTIRLERLASQRNILGLKE 158

Query: 155 LTSKDVETELSQIQETDSFLDEIGVIGREIEVCEIVNKLIDLSSQE-ALSVLPIVGEGGL 214
           LT+   +  L     T S +DE  V GR+ +  EI+  LI  + ++  ++V+ IVG GG+
Sbjct: 159 LTAMIPKQRL----PTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 218

Query: 215 GKTALAKVIFNHEVIRENFDTTIWVYVSRHFVIKKILKAILETLNANFGGLDSKEAILQE 274
           GKT L+++++N + +R  F T +W +VS  F + KI K + E++ +        + +  +
Sbjct: 219 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 278

Query: 275 LKKSLSDK--RYFLVLDDVWNENPNVWNELKGCLLKVNKNFGSVIVVTTRSDEVAKIVET 334
           LK+ L+     + LVLDD+WNEN   W+ L+   +   +  GS I+VTTRS  VA I+  
Sbjct: 279 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQ--GSQILVTTRSQRVASIM-C 338

Query: 335 QCCRHDLRKLSNDQCWSLFEKCIFANELP-KTLKMDKVREELIEQFGGMPLVVKALGGMV 394
               H+L+ LS+  CWSLF K +F N+ P    ++  + E ++ +  G+PL VK LGG++
Sbjct: 339 AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 398

Query: 395 KLDKNHEELESTLKNLI-RIPSNENHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFKFK 441
           + +    E E  L + I  +P+++++ L  +++S   LP + LK+ FAY S F +   F+
Sbjct: 399 RFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 445

BLAST of Spg005307 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 157.5 bits (397), Expect = 2.4e-38
Identity = 130/454 (28.63%), Postives = 229/454 (50.44%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAE + SF V+    K+ +L + +     G  +++  LK  L  ++++L D    K + +
Sbjct: 1   MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
            V+ ++E ++D+V++AD +++    ++LR      GKE  +   V +    LV R K A+
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELR------GKEKGIKKQVRTLACFLVDRRKFAS 120

Query: 121 RIKAVAKALHEHYRAASVVGLVALT-----SKDVETELSQIQETDSFLDEIGVIGREIEV 180
            I+ + K + E       +G+  +      S  ++    +I++T S   E  ++G +  V
Sbjct: 121 DIEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSV 180

Query: 181 CEIVNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHFVI 240
            E+V+ L++    +++ V+ + G GG+GKT LA+ +F+H+++R +FD   WV VS+ F  
Sbjct: 181 EELVDHLVE---NDSVQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTR 240

Query: 241 KKILKAILETLNA-NFGGLDSKEAILQ-ELKKSLSDKRYFLVLDDVWNENPNVWNELKGC 300
           K + + IL+ L   + G +   E  LQ EL + L   RY LVLDDVW E    W+ +K  
Sbjct: 241 KDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAV 300

Query: 301 LLKVNKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKTLK 360
                   G  +++T+R++ +    +  C     R L+ +Q W LFE+ + +       K
Sbjct: 301 F---PHKRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKTEFK 360

Query: 361 MDK-VREELIEQFGGMPLVVKALGGMVKLDKNHEELE--STLKNLIR--------IPSNE 420
           +D+ + +E++   GG+PL VK LGG+  L K H  LE      N++            N 
Sbjct: 361 VDEAMGKEMVTYCGGLPLAVKVLGGL--LAKKHTVLEWKRVHSNIVTHIVGKSGLSDDNS 420

Query: 421 NHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFK 437
           N     + LS + LP   LK  F Y + F +D+K
Sbjct: 421 NSVYRVLSLSYEDLP-MQLKHCFFYLAHFPEDYK 433

BLAST of Spg005307 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 152.9 bits (385), Expect = 5.9e-37
Identity = 109/410 (26.59%), Postives = 207/410 (50.49%), Query Frame = 0

Query: 35  LSKLKDSLLMVEAILHDVHKIKLDHQAVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVG 94
           L +LK +L+    +L D  +     + VK W+  ++D  F+A+ +LD+L  + LRR+VV 
Sbjct: 36  LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95

Query: 95  TGKEAMVSYFVSSSKNPLVFRLKMANRIKAVAKALHEHYRAASVVGLVALTSKDVETELS 154
                +   F +        + K+  +++ V + L  H +   V+GL   +         
Sbjct: 96  EA-GGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQ 155

Query: 155 QIQETDSFLDEIGVIGREIEVCEIVNKLI--DLSSQEALSVLPIVGEGGLGKTALAKVIF 214
             +     L +  ++GR  +   +VN L+  D  S    +V+ +VG  G+GKT L +++F
Sbjct: 156 ASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVF 215

Query: 215 NHEVIRENFDTTIWVYVSRHFVIKKILKAILETLNANFGGLDSKEAILQELKKSLSDKRY 274
           N   + E+F+  +W+    +F +  + KA+L+ + ++    +   ++  +LKK+LS KR+
Sbjct: 216 NDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRF 275

Query: 275 FLVLDDVWNENPNVWNELKGCLLKVNKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSN 334
            LVLDD W+E+ + W   +       +  GS IV+TTRS+ V+ + + +   + ++ ++N
Sbjct: 276 LLVLDDFWSESDSEWESFQVAFTDAEE--GSKIVLTTRSEIVSTVAKAEKI-YQMKLMTN 335

Query: 335 DQCWSLFEKCIFANELPKTL--KMDKVREELIEQFGGMPLVVKALGGMVKLDKNHEELES 394
           ++CW L  +  F N    ++  +++ + + + EQ  G+PL  +A+   ++   N ++  +
Sbjct: 336 EECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYA 395

Query: 395 TLKNLIRIPSNENHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFKFKKE 441
             KN     S  N  L  +KLS D LP   LK+ FA  S F +   F +E
Sbjct: 396 VSKN---FSSYTNSILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFDRE 437

BLAST of Spg005307 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 145.6 bits (366), Expect = 9.5e-35
Identity = 127/456 (27.85%), Postives = 227/456 (49.78%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAE + SF VQ+ L  ++   +E++    G  +++  LK  L  ++++L D    K    
Sbjct: 1   MAEGVVSFGVQK-LWALLNRESERLN---GIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
            V+ ++E ++D+VF+A+ +++    + LR    G GK   V   V      L  R K+A+
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLR----GEGKG--VKNHVRRLACFLTDRHKVAS 120

Query: 121 RIKAVAKALHEHYRAASVVGLV--------ALTSKDVETELSQIQETDSFLDEIGVIGRE 180
            I+ + K + +       +G+         +L+ +D++ E+ Q     S  D +GV   E
Sbjct: 121 DIEGITKRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQREIRQTFPNSSESDLVGV---E 180

Query: 181 IEVCEIVNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRH 240
             V E+V  ++++ +   + V+ I G GG+GKT LA+ IF+H+++R +FD   WV VS+ 
Sbjct: 181 QSVEELVGPMVEIDN---IQVVSISGMGGIGKTTLARQIFHHDLVRRHFDGFAWVCVSQQ 240

Query: 241 FVIKKILKAILETLNANFGG-LDSKEAILQ-ELKKSLSDKRYFLVLDDVWNENPNVWNEL 300
           F  K + + IL+ L  + G  L   E  +Q +L + L   RY +VLDDVW E    W+ +
Sbjct: 241 FTQKHVWQRILQELRPHDGEILQMDEYTIQGKLFQLLETGRYLVVLDDVWKEED--WDRI 300

Query: 301 KGCLLKVNKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPK 360
           K       +  G  +++T+R++ V    +  C     R L+  + W LFE+ +      +
Sbjct: 301 KEVF---PRKRGWKMLLTSRNEGVGLHADPTCLSFRARILNPKESWKLFERIVPRRNETE 360

Query: 361 TLKMDKVREELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNL--------IRIPSNE 420
             +M+ + +E++   GG+PL VK LGG++       E +   +N+            ++ 
Sbjct: 361 YEEMEAIGKEMVTYCGGLPLAVKVLGGLLANKHTASEWKRVSENIGAQIVGKSCLDDNSL 420

Query: 421 NHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFKFK 439
           N     + LS + LP + LK  F Y + F +D+K K
Sbjct: 421 NSVYRILSLSYEDLP-TDLKHCFLYLAHFPEDYKIK 434

BLAST of Spg005307 vs. TAIR 10
Match: AT5G48620.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-33
Identity = 125/457 (27.35%), Postives = 223/457 (48.80%), Query Frame = 0

Query: 1   MAEFLWSFAVQEVLKKIVKLAAEQIGLAWGFKKELSKLKDSLLMVEAILHDVHKIKLDHQ 60
           MAE   SF     L+K+  L + +     G  ++L  LK  L  ++++L D    K    
Sbjct: 1   MAEGFVSFG----LEKLWDLLSRESERLQGIDEQLDGLKRQLRSLQSLLKDADAKKHGSD 60

Query: 61  AVKLWVEKLEDVVFEADVLLDKLAYDDLRRKVVGTGKEAMVSYFVSSSKNPLVFRLKMAN 120
            V+ ++E ++D+VF+A+ +++    + LR    G GK   V   V      L  R K+A+
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLR----GEGKG--VKKHVRRLARFLTDRHKVAS 120

Query: 121 RIKAVAKALH------EHYRAASVV-GLVALTSKDVETELSQIQETDSFLDEIGVIGREI 180
            I+ + K +       + +    ++ G+ +L+ ++ +    +I++T     E  ++G E 
Sbjct: 121 DIEGITKRISDVIGEMQSFGIQQIIDGVRSLSLQERQRVQREIRQTYPDSSESDLVGVEQ 180

Query: 181 EVCEIVNKLIDLSSQEALSVLPIVGEGGLGKTALAKVIFNHEVIRENFDTTIWVYVSRHF 240
            V E+V  L++    +   V+ I G GG+GKT LA+ +F+H+++R +FD   WV VS+ F
Sbjct: 181 SVEELVGHLVE---NDIYQVVSIAGMGGIGKTTLARQVFHHDLVRRHFDGFAWVCVSQQF 240

Query: 241 VIKKILKAILETLNANFGG-LDSKEAILQ-ELKKSLSDKRYFLVLDDVWNENPNVWNELK 300
            +K + + IL+ L  + G  L   E+ LQ +L + L   RY LVLDDVW +    W+ +K
Sbjct: 241 TLKHVWQRILQELQPHDGNILQMDESALQPKLFQLLETGRYLLVLDDVWKKED--WDRIK 300

Query: 301 GCLLKVNKNFGSVIVVTTRSDEVAKIVETQCCRHDLRKLSNDQCWSLFEKCIFANELPKT 360
                  +  G  +++T+R++ V    +  C       L+ ++ W L E+ +F       
Sbjct: 301 AVF---PRKRGWKMLLTSRNEGVGIHADPTCLTFRASILNPEESWKLCERIVFPRRDETE 360

Query: 361 LKMDKVRE----ELIEQFGGMPLVVKALGGMVKLDKNHEELESTLKNL--------IRIP 420
           +++D+  E    E++   GG+PL VKALGG++       E +    N+            
Sbjct: 361 VRLDEEMEAMGKEMVTHCGGLPLAVKALGGLLANKHTVPEWKRVSDNIGSQIVGGSCLDD 420

Query: 421 SNENHALSTMKLSVDRLPSSSLKQSFAYRSKFSQDFK 437
           ++ N     + LS + LP + LK  F Y + F +D K
Sbjct: 421 NSLNSVNRILSLSYEDLP-THLKHRFLYLAHFPEDSK 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145226.15.9e-18076.80putative disease resistance protein RGA3 [Momordica charantia][more]
XP_004152291.11.2e-16773.03putative disease resistance protein RGA3 [Cucumis sativus] >KGN53095.1 hypotheti... [more]
XP_038874765.11.1e-16570.43putative disease resistance protein RGA3 [Benincasa hispida][more]
XP_022145225.17.0e-16570.72putative disease resistance protein RGA3 [Momordica charantia][more]
XP_008453966.17.8e-16469.61PREDICTED: putative disease resistance protein RGA3 [Cucumis melo] >TYK16977.1 p... [more]
Match NameE-valueIdentityDescription
Q7XA401.2e-5533.49Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA427.3e-4831.64Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ91.2e-4732.41Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA391.7e-4430.73Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR46.0e-4230.33Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1CVQ52.9e-18076.80putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A0A0KTZ75.6e-16873.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015850 PE=4 SV=1[more]
A0A6J1CTV33.4e-16570.72putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5D3CZ173.8e-16469.61Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7TSS63.8e-16469.61Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
AT3G14470.14.2e-4330.33NB-ARC domain-containing disease resistance protein [more]
AT1G53350.12.4e-3828.63Disease resistance protein (CC-NBS-LRR class) family [more]
AT3G14460.15.9e-3726.59LRR and NB-ARC domains-containing disease resistance protein [more]
AT5G35450.19.5e-3527.85Disease resistance protein (CC-NBS-LRR class) family [more]
AT5G48620.13.1e-3327.35Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 378..398
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 193..208
score: 45.16
coord: 365..379
score: 33.17
coord: 266..280
score: 46.5
NoneNo IPR availableGENE3D1.20.5.4130coord: 8..123
e-value: 8.2E-15
score: 56.8
NoneNo IPR availablePANTHERPTHR23155:SF1139NBS-LRR TYPE DISEASE RESISTANCE PROTEINcoord: 7..440
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 329..416
e-value: 5.3E-6
score: 27.9
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 175..409
e-value: 3.9E-48
score: 163.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 151..324
e-value: 5.1E-36
score: 125.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 158..427
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 9..99
e-value: 5.1E-18
score: 65.1
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 7..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg005307.1Spg005307.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding