Homology
BLAST of Spg005273 vs. NCBI nr
Match:
XP_038877313.1 (putative disease resistance protein RGA3 [Benincasa hispida])
HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 695/1048 (66.32%), Postives = 826/1048 (78.82%), Query Frame = 0
Query: 62 ENKLREMAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINR 121
+ K + MAEFLW FAVQEVLKK +K+AAE+I +AWG +KELSKLR+ LL +EAILRDIN+
Sbjct: 118 KQKAKRMAEFLWIFAVQEVLKKTVKVAAEEISMAWGFKKELSKLRKCLLKSEAILRDINK 177
Query: 122 KRLHHQSVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLF 181
K+LHH+SV MWV++LQ VYE DDLLDELVYE LRQKV+ EK +KVRNFFS SNNPF F
Sbjct: 178 KKLHHESVRMWVEDLQHFVYEVDDLLDELVYEHLRQKVDTEKRNKKVRNFFSSSNNPFPF 237
Query: 182 RWKMAKKMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFE--V 241
R KMAK+MKDITETL +HY +AS LG+V L+GEES ET+KNI+LKQ RETTS LNFE +
Sbjct: 238 RRKMAKRMKDITETLYKHYCEASPLGLVALLGEESIETHKNINLKQIRETTSNLNFEEVI 297
Query: 242 IGREVEASNILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVC 301
IGREVE SNI+K+VI+SSN+H MSIIPIVGMGG+G+TTLAK++FNHELVK HFD T+WVC
Sbjct: 298 IGREVEVSNIVKMVIESSNEHPMSIIPIVGMGGLGETTLAKMVFNHELVKDHFDETIWVC 357
Query: 302 VSEPFIITNILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWG 361
VSEPFII NILEG+FQSLT TS G +KENL+R L++EMQGK+Y LVLD VWNEEA LWG
Sbjct: 358 VSEPFIILNILEGMFQSLTKTSSG-LNKENLLRKLQEEMQGKSYLLVLDYVWNEEARLWG 417
Query: 362 ELKDCLKETAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLL 421
EL++CLK AGK GN IIVTTRS +VAT+METTSSHHL KLSDDQCWSLFK+SA+ANGL
Sbjct: 418 ELRECLKNIAGKFGNSIIVTTRSAEVATVMETTSSHHLTKLSDDQCWSLFKESASANGLS 477
Query: 422 MNQELETVRVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLS 481
M+ +LE ++ ++++I GVPLVAKVLGGA FEGN+ERW+T+V+ VV NIS DDKDFV S
Sbjct: 478 MSPKLEIIQKLVIRRIGGVPLVAKVLGGATKFEGNYERWLTQVESVVTNISNDDKDFVFS 537
Query: 482 ILKLSVDRLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIG 541
LKLSVD LPL SLKQCFAYCSNFPKD+ F KE+LI+MWIAQGF+Q+QKG N M+ E+IG
Sbjct: 538 TLKLSVDCLPLFSLKQCFAYCSNFPKDYEFAKETLIQMWIAQGFIQAQKGNNTMLEENIG 597
Query: 542 EGYFNLLLSRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKLELDLPNWSEKGARN 601
E Y N LLSRSLFQDV D G + +FKMHDLIHD++C+IS+HQ E NWSEK R
Sbjct: 598 EDYINFLLSRSLFQDVSKDIDGTVTHFKMHDLIHDIACVISSHQNFEFKHSNWSEKSTRK 657
Query: 602 FRTLVNDNYKVLHTHKLDFLCLRVLVLN-PFDKIELLNSIGMLKHLRYLEII-NYVGRLP 661
RT V +N K++H +D LRVLV+N PF +I L +SIGMLKHLRYL+II + + +LP
Sbjct: 658 LRTTVINN-KMIHDKLVDLGSLRVLVMNSPFLRI-LPDSIGMLKHLRYLDIIGSSILKLP 717
Query: 662 ESITMLYNLQTLNLGTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFV 721
ESITMLYNLQTL LGTRVDLPKNLRKLV LRHL+FF ++QMP +SQLIHLQTL AFV
Sbjct: 718 ESITMLYNLQTLKLGTRVDLPKNLRKLVMLRHLQFFYIYIKQMPLCISQLIHLQTLPAFV 777
Query: 722 VGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYE 781
VG ++GC IEELGPL NLKGSL LS+L RVKSK EAI AKLM K++L L F W + E
Sbjct: 778 VGSEKGCKIEELGPLNNLKGSLQLSSLNRVKSKKEAIAAKLMGKKNLCELTFEWDVYSEE 837
Query: 782 EEDNN-HNDLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPM 841
E+ NN +ND EVLEGLQPHKNL+SLS++ +GGQVLP+ IFVENLV I L +C CE LPM
Sbjct: 838 EKHNNCNNDFEVLEGLQPHKNLRSLSVVHYGGQVLPNGIFVENLVIIFLQECNRCEMLPM 897
Query: 842 LGQLPNLENLYISGMGSVRSIGSEFYGNN--SNQRVYFPNLKTLSFNSMRNLQLWEKVTN 901
LGQLPNLE L+IS MGSVRSIGSEFYGN SNQRV FP LK LSF M+NL+ WE VT+
Sbjct: 898 LGQLPNLEELHISSMGSVRSIGSEFYGNTHYSNQRVSFPKLKELSFRLMQNLEQWEDVTD 957
Query: 902 GTAFPRLEGLGINHCSKLMRIPDQFESLQVLRIFECEGFIVNEPPNVHC----KYLQIYN 961
G AFP L+ L I CSKL+ +P F SL+ LRI CE + P ++C + L+I
Sbjct: 958 GIAFPYLQSLSIVICSKLLDVPHHFVSLRTLRIVGCEE-LAKLPNGLNCPNSIESLKISE 1017
Query: 962 CPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHF 1021
CPNL LN HNMHN KE++IVG +Q +D P L Q S + +L L+ L T+LP+ L++
Sbjct: 1018 CPNLALNLHNMHNLKEMEIVGCMQDYDFSPLLHQSSILTHLSLIDSLRNATELPRQLKYL 1077
Query: 1022 IALKSLTINDFRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRR 1081
ALK+LTI +F SLE LP+WLGNLTSLE+L L C LK PSKE ML L KLNHL+VR
Sbjct: 1078 TALKALTIKNFNSLEALPDWLGNLTSLETLGLFDCKNLKCFPSKEVMLCLKKLNHLHVRG 1137
Query: 1082 CRQLQLGRDHPEGAKLSHFLISNENVRF 1099
C L LG+D PE AKLSH + +VRF
Sbjct: 1138 CPMLLLGQDDPEKAKLSHLPNNCVDVRF 1161
BLAST of Spg005273 vs. NCBI nr
Match:
XP_022145229.1 (putative disease resistance protein RGA4 [Momordica charantia])
HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 675/1033 (65.34%), Postives = 792/1033 (76.67%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVLKKVLKLAAEQIGVAW L+KELSKLR+ LL AE IL DINR++L H+
Sbjct: 1 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +WV++LQ +VY+ADDLLD+LVYEDLRQKVE+EK KVRNF SPSNNPFLFR+KMAK
Sbjct: 61 SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKI-NKVRNFLSPSNNPFLFRFKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
+MK I+ETL HY +AS V+G VGEESTET L+Q RETTSILNF+VIGRE+E
Sbjct: 121 RMKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVL 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
I+KLVIDSS++H MSIIPIVGMGG+GKTTLAK++FNHEL+K HFD T+WVCVS+PFII
Sbjct: 181 KIMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIV 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
NILEGIFQSLTNTS G KE L L+KEMQGK YFLVLDDVWNEE LW EL+DCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
A KSGN IIVTTRS VATIM+T SH L KLSDD+CWSLFK+SANANGLLMN ELE +
Sbjct: 301 IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 487
R +VK+I G+PLVAKVLGG FEGN+ERWVT V+ +V+NI D+KDFVLS LKLSVD
Sbjct: 361 REVVVKRIGGIPLVAKVLGGTAKFEGNYERWVTTVESIVRNILNDEKDFVLSTLKLSVDS 420
Query: 488 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLL 547
LPLLSLKQCFA+CSNFPKD F KE+LI+MWIAQGF+Q ++G+N ++ E+IGEGYFN LL
Sbjct: 421 LPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFNFLL 480
Query: 548 SRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKLELDLPNWSEKGARNFRTLVNDN 607
SRSLFQDV D G I +FKMHDLIHDV+CI+SN QKLELD NWSEKG R RTLV +N
Sbjct: 481 SRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRALRTLVINN 540
Query: 608 YKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEII-NYVGRLPESITMLYNL 667
++H D + LRVLVLN L +SIGMLKHLRYL+II + + RLPESITMLYNL
Sbjct: 541 GAIIHNKLEDSVYLRVLVLNSDFIKNLPDSIGMLKHLRYLDIIGSRIRRLPESITMLYNL 600
Query: 668 QTLNLGTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFDEGCTI 727
Q L L TR+DLPKNLRKLV LRHL FF +++MP H+SQLIHLQTL ++VGF++G I
Sbjct: 601 QILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYIVGFEKGRKI 660
Query: 728 EELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEED-NNHND 787
EELGPL +LKGSL L NLERVKSK EAI AKL+EK +L L W + + EE NN ND
Sbjct: 661 EELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTLHWNSSSCGEEGYNNRND 720
Query: 788 LEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQLPNLEN 847
EVLEGLQPH+NLQSLSI FGG LP+ +FVENLVAI+L +C CE LPMLGQLPNLE
Sbjct: 721 SEVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAIVLQECNKCEMLPMLGQLPNLEE 780
Query: 848 LYISGMGSVRSIGSEFYGNNSNQR--VYFPNLKTLSFNSMRNLQLWEKVTNGTAFPRLEG 907
L +S MGSVR IGSEFYGNNSN R ++FP L+ LSF+ M L+ WE+++NG FP LE
Sbjct: 781 LRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFSHMEKLEQWEELSNGINFPHLET 840
Query: 908 LGINHCSKLMRIPD-QFESLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNGHNMH 967
L + +CSKL +PD +F SL+ LRI CE C +++I CP L LN HNM
Sbjct: 841 LSMFYCSKLTSVPDHEFVSLRTLRIVGCEELSKLPSKLECCNFMKISGCPKLRLNMHNMC 900
Query: 968 NFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHFIALKSLTINDFR 1027
N EL+I G ++ +D L+ QSSV N+ L L TQLPQ LQH LKSL I F
Sbjct: 901 NLNELEINGPMEEYDFS-FLMHQSSVTNIFLTDSLRNATQLPQQLQHLTTLKSLAIEYFN 960
Query: 1028 SLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQLQLGRDHPE 1087
+E LPEWL L +LE++ + C LK+LPS++AMLRLTKL HL VR C L LG+ E
Sbjct: 961 GVEALPEWLEKLANLETMGVFECKNLKRLPSQQAMLRLTKLKHLRVRGCPLLPLGQGDLE 1020
Query: 1088 GAKLSHFLISNEN 1096
AKLSH I+ +N
Sbjct: 1021 RAKLSHLPITVDN 1027
BLAST of Spg005273 vs. NCBI nr
Match:
XP_022145177.1 (putative disease resistance protein RGA3 [Momordica charantia])
HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 636/1027 (61.93%), Postives = 755/1027 (73.52%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVL+K++K+AA+QIG+AWGLEKELSKL + LL AE IL DINR++LHH
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +W+++LQD+V+EA DLLDELVYEDLR+KVEI K KVR S S N F FR KMAK
Sbjct: 61 SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEIGK-MNKVRGSISRSYNLFSFRRKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
KMKD+TETL +HY +AS L GLVG+ES ETN+ I LKQ RETTS LNFEV+GRE+E S
Sbjct: 121 KMKDVTETLYKHYCEASPL---GLVGDESIETNQ-IVLKQIRETTSNLNFEVVGREIEVS 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
NI+KLVIDSSN+H SIIPIVGMGG+GKTTLA++IFNHE++KGHFD T+W+CVSEPFII
Sbjct: 181 NIVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIII 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
NILEGIFQ LT TS G SKE LV+ L++EM GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 NILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQ 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
KSGN IIVTTRS +VAT +ET SSHHL KLSDDQ W LFK SANANGL MN +L+ +
Sbjct: 301 ITEKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDII 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 487
R LVKKI GVPLVAKVLGGA+ FEG++E WV +VK VV+NISKDDKDFVLSILKLSVD
Sbjct: 361 REILVKKIGGVPLVAKVLGGAIKFEGDYETWVKKVKSVVRNISKDDKDFVLSILKLSVDC 420
Query: 488 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLL 547
LPLLSLKQCFAYCSNF KD +F+KE+LI+MWIAQGF+Q +G NE EDIGE + N LL
Sbjct: 421 LPLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNFLL 480
Query: 548 SRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQK-LELDLPNWSEKGARNFRTLVND 607
SRSLFQDV D R+ +FKMHDLIHD++C ISNH K LEL NWS K AR RTL+ +
Sbjct: 481 SRSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLICN 540
Query: 608 NYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEIINYVGR-LPESITMLYN 667
N +V++ +D + LRVLVLN LL+SIG LKHLRYL+I +Y R LPESI LYN
Sbjct: 541 N-QVIYNELMDCVSLRVLVLNSAKTDNLLDSIGKLKHLRYLDISHYSIRVLPESIAKLYN 600
Query: 668 LQTLNLGTRVDLPKNLRKLVRLRHLKFFR-FRVQQMPSHMSQLIHLQTLDAFVVGFDEGC 727
LQTL +G LPKNLRK+V LRHLKF+ + QMPSHMS LIHLQTL F GF+ GC
Sbjct: 601 LQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAGFENGC 660
Query: 728 TIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDNNHN 787
IEELGPLKNLKG L LSNLERVKSK EAI AKL+EK++L L F W ++++E D +
Sbjct: 661 KIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDW-SLDFERND---D 720
Query: 788 DLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQLPNLE 847
D EVLEGLQPHKNLQSL+I+ FGGQVLP IFVENL I L CE LPMLGQLPNL
Sbjct: 721 DSEVLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAVIRLCGGKKCEMLPMLGQLPNLV 780
Query: 848 NLYISGMGSVRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQLW-EKVTNGTAFPRLEG 907
L IS M SVR IGSEFYGN S F L+ F + NL+ W E + +F L+
Sbjct: 781 ELEISFMDSVRCIGSEFYGNGS-----FSKLRKFDFIGLHNLEQWTEAASISNSFGSLQT 840
Query: 908 LGINHCSKLMRIPDQFE---SLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNGHN 967
L + C L ++P+ E SL L + +C +N + L I+ L L +
Sbjct: 841 LNVIGCGNLKKLPNGLEFCSSLHDLTVRDCPKLALNVQNMHNLSNLFIHGFKELPLGLSH 900
Query: 968 MHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHFIALKSLTIND 1027
+ N KE+++VG +Q +D P L Q S+I L L T+LPQ ++ AL++LTI +
Sbjct: 901 LTNLKEMEVVGCMQDYDFSPFLSLQ-SLIKLHLNDVSRICTELPQHFENLTALETLTIVN 960
Query: 1028 FRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQLQLGRDH 1087
F ++E LPEWL NL SLE+L L C LK+LPS+EAMLRLTKL HL V RC QL D
Sbjct: 961 FDAIEALPEWLENLASLETLNLEDCKNLKRLPSREAMLRLTKLEHLKVFRCPQLPGRGDL 1011
BLAST of Spg005273 vs. NCBI nr
Match:
XP_038895409.1 (disease resistance protein RGA2-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 633/1032 (61.34%), Postives = 746/1032 (72.29%), Query Frame = 0
Query: 70 EFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQSV 129
EFLWTF VQE LKKV+K+AA++I VAWG +KEL KL + LL +EAILRDI K+L+++ V
Sbjct: 5 EFLWTFVVQEALKKVVKVAAQEIAVAWGFKKELEKLTKWLLKSEAILRDIITKKLYNEPV 64
Query: 130 SMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAKKM 189
MWV++LQ VYEADDLLDELVYEDLR+K E EK ++KV NFFS S+NPFLFR KMAK+M
Sbjct: 65 RMWVEDLQHFVYEADDLLDELVYEDLRRKAETEKIEKKVHNFFSCSHNPFLFRRKMAKRM 124
Query: 190 KDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFE--VIGREVEAS 249
KDITETL +HY +AS LG+V LVG ESTE KNIDLKQ R+TTS L E +IGREVE S
Sbjct: 125 KDITETLYKHYCEASPLGLVALVGHESTEARKNIDLKQIRDTTSKLKPEEVIIGREVEVS 184
Query: 250 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 309
NI+KLVI+SSN+H MSIIPIVGMGG+GKTTLAK++FNH LV+ HFD TLWVCVSEPFII
Sbjct: 185 NIVKLVIESSNEHPMSIIPIVGMGGLGKTTLAKMVFNHGLVQTHFDKTLWVCVSEPFIII 244
Query: 310 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 369
NILEGIFQSLT TS +KENL++ L++EM+G NYFLVLDDVWNEEA LW LKDCL E
Sbjct: 245 NILEGIFQSLTKTSSSCLNKENLLQRLQEEMRGNNYFLVLDDVWNEEAILWDNLKDCLNE 304
Query: 370 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 429
AGK GN IIVTTRS VAT+M TTSS HL KLSDD CWSLFK +A+GL ELE +
Sbjct: 305 IAGKPGNTIIVTTRSPDVATVMGTTSSIHLKKLSDDLCWSLFKKGVSADGL----ELEII 364
Query: 430 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 489
R E+V KI G+PLVAKVLGGAV FEGN+ERW+T+ K VVKNISKDDKDF+ SILKLSVD
Sbjct: 365 REEVVTKIGGIPLVAKVLGGAVKFEGNYERWMTKFKSVVKNISKDDKDFISSILKLSVDC 424
Query: 490 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLL 549
LPLLSLKQCFAYCSNFPKD+ F KESLI+ WIAQGF+Q Q+ +NE+MMEDIGEGYF+LLL
Sbjct: 425 LPLLSLKQCFAYCSNFPKDYKFCKESLIQTWIAQGFIQPQEEDNELMMEDIGEGYFDLLL 484
Query: 550 SRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKLELDLPNWSEKGARNFRTLVNDN 609
SRSLF+ V D R +FKMHDLIHD++C ISN ++E +L N SEK R RT+++D
Sbjct: 485 SRSLFEVVDEDSNERTTHFKMHDLIHDIACAISN--QVESNLHNSSEKSVRKLRTIIHDI 544
Query: 610 YKVLHTHKLDFLCLRVLV--LNPFDKIELLNSIGML-KHLRYLEIINYVGRLP---ESIT 669
+ D +CLRVLV L+P + L NSIG++ KHLRYLEI L ESI
Sbjct: 545 DNSICDKFEDLVCLRVLVFALHPGWDVSLFNSIGIIAKHLRYLEIKQCYHELKYSLESIA 604
Query: 670 MLYNLQTLNLGTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFD 729
MLYNLQTL LG LP NLRKLV+LRHL+F +MP HM QLIHLQTL AF VG +
Sbjct: 605 MLYNLQTLKLGVGASLPTNLRKLVKLRHLEFKDIGDGKMPLHMRQLIHLQTLTAFFVG-E 664
Query: 730 EGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDN 789
+GC IEELGPLKNLKGSL L +L VKSK EA+ A+L+EK++L +L F W + + EE++
Sbjct: 665 DGCKIEELGPLKNLKGSLDLGSLGEVKSKEEAMGARLIEKKNLTKLAFYWNSSS--EEED 724
Query: 790 NHNDLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQLP 849
N N+LEVLEGLQPH NLQSLSI+ + GQ P+NIFVE LV I+LH C CE LPMLGQLP
Sbjct: 725 NLNELEVLEGLQPHNNLQSLSILHYNGQAFPNNIFVEGLVHIMLHGCMKCEMLPMLGQLP 784
Query: 850 NLENLYISGMGSVRSIGSEFYG-NNSNQRVYFPNLKTLSFNSMRNLQLWEKVTNGTAFPR 909
NLE L IS + SV+SIG EFYG NNSNQRV FP LK S M NL+ WE V NG AF
Sbjct: 785 NLEELVISEVDSVKSIGREFYGSNNSNQRVCFPKLKKFSLMYMENLENWEDVPNGMAFSH 844
Query: 910 LEGLGINHCSKLMRIPDQFESLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNGHN 969
L L + C KL IP FE L + K L +CP L N N
Sbjct: 845 LTILYMRGCPKL-SIPHHFECLNSI------------------KSLTHIHCPKLPQNMCN 904
Query: 970 MHN-FKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLV--TTTQLPQLLQHFIALKSLT 1029
+N ++++I G ++ +D P LI QSS+ + L +LPQ L + LK L
Sbjct: 905 QYNKLEDMEIWGCMKDYDFSP-LIHQSSITRVSLTDTSEDNPAIELPQQLGYLTTLKLLE 964
Query: 1030 INDFRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQLQLG 1089
I F +E LP+WL NL LE L+L YC LK LPSK ML LTKLN + V+ C L LG
Sbjct: 965 IFGFEGIEALPDWLENLACLEELKLYYCKNLKWLPSKHVMLSLTKLNRVEVKGCPLLPLG 1007
BLAST of Spg005273 vs. NCBI nr
Match:
XP_022145104.1 (disease resistance protein RGA2-like [Momordica charantia])
HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 622/1023 (60.80%), Postives = 745/1023 (72.83%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVL+K++K+AA+QIG+A GLEKELS L + L AE ILRDINRK+ H
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSHSD 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +WV++LQD+V+EADDLLDELVYE LR+KVEI K KVR+ FS S+N FLFR KMAK
Sbjct: 61 SVRLWVEDLQDVVHEADDLLDELVYEHLRRKVEIGKI-NKVRDSFSTSHNVFLFRLKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
K+KDIT+ L +HY +AS L GLVG+ESTE N+ I L Q R+TTS LNFEV+GRE E S
Sbjct: 121 KIKDITQILHKHYCEASPL---GLVGQESTEANQ-IVLSQIRDTTSNLNFEVVGREDEVS 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
NI+KLVIDSSN+H MSI+PIVGMGG+GKTT A+ IFNH+ +KGHFDN +WVCVSEPFII
Sbjct: 181 NIVKLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIII 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
NILEGIFQSLT TS G K L+ ++KEM GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 NILEGIFQSLTKTSSGLNHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQ 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
AGKSGN IIVTTRS +VA I+ T SH L KLSDDQCW LFK+SANAN L MN +LE +
Sbjct: 301 IAGKSGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKESANANRLSMNPKLEII 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 487
R LVKKI GVPLVAKVLGGA FEG++ERWVT+V+ VV+NIS DDKDFV SILKLSVD
Sbjct: 361 RDMLVKKIGGVPLVAKVLGGATKFEGDYERWVTKVESVVRNISNDDKDFVSSILKLSVDC 420
Query: 488 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFV--QSQKGENEMMMEDIGEGYFNL 547
LPLLSLKQCFAYCSNF KD L++KE+LIR+WIAQGF+ Q Q+G+NEM EDIGE +FN
Sbjct: 421 LPLLSLKQCFAYCSNFAKDCLYDKENLIRIWIAQGFIQPQDQEGDNEMTEEDIGEWHFNF 480
Query: 548 LLSRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKL--ELDLPNWSEKGARNFRTL 607
LLSRSLFQDV MDK R YF MHDLIHD++C ISNHQKL EL N S K AR RTL
Sbjct: 481 LLSRSLFQDVTMDKSERGIYFIMHDLIHDIACAISNHQKLLHELSSSNRSRKSARKLRTL 540
Query: 608 VNDNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEII--NYVGRLPESIT 667
+ +N +V++ +D + LRVLVL+ LL SIG LKHLRYL I NYV LPESI
Sbjct: 541 ICNN-QVIYNELVDCVSLRVLVLHCEKTGNLLESIGKLKHLRYLSIANGNYVDVLPESIV 600
Query: 668 MLYNLQTLNLGTRVDLPKNLRKLVRLRHLKF-FRFRVQQMPSHMSQLIHLQTLDAFVVGF 727
MLYNLQTL L LPKN RK+V LRHL+F + +QMPSHMS+LIHLQTL FV GF
Sbjct: 601 MLYNLQTLKLRHMKSLPKNSRKMVSLRHLEFELKSDFRQMPSHMSELIHLQTLSRFVAGF 660
Query: 728 DEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEED 787
+ GC IEELGPLKNLKG L LS LERV+SK E+I AKL+EK++L+ L F W + +E ++
Sbjct: 661 ENGCKIEELGPLKNLKGKLQLSCLERVESKEESIVAKLVEKKNLQELIFEWSS-RFEGKN 720
Query: 788 NNHNDLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQL 847
+ +++LEVLEGLQPHKNLQSL++IGFGGQVL IFVENL I L C +CE+LPMLG+L
Sbjct: 721 SRYSELEVLEGLQPHKNLQSLTVIGFGGQVLGDAIFVENLTVIRLSNCKNCERLPMLGRL 780
Query: 848 PNLENLYISGMGSVRSIGSEFYGNNSNQRVY-FPNLKTLSFNSMRNLQLW-EKVTNGTAF 907
PNL L I+ M S+R IGSEFYGN SN + + F L+ L+F RNL+ W + +F
Sbjct: 781 PNLVELNINSMDSLRCIGSEFYGNGSNNQGHSFSKLRKLTFIWCRNLEQWKDAAVVSNSF 840
Query: 908 PRLEGLGINHCSKLMRIPDQFE---SLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLT 967
L+ L I C L ++P+ E SL+ L + C +N + L+I L
Sbjct: 841 GSLQTLSIYGCGNLEKLPNGLEGCSSLRDLTVAYCPKLSLNVQNCQNLSNLRIDGLKKLP 900
Query: 968 LNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLV-TTTQLPQLLQHFIALK 1027
L ++ N KE+K VG +Q +D P + QS +I L L TQLP LQH ALK
Sbjct: 901 LGLGHLTNLKEMKFVGCMQDYDFTPFVSLQSLIIKLELRDECSGNCTQLPPHLQHLTALK 960
Query: 1028 SLTINDFRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQL 1078
L+I +F +EV PEWL NLTSLE L C L++LPS EA+ RLTKL L +C L
Sbjct: 961 QLSIGNFGGIEVPPEWLNNLTSLEMLSFEGCENLRRLPSTEAIRRLTKLELLSAIKCPLL 1016
BLAST of Spg005273 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 440.7 bits (1132), Expect = 5.0e-122
Identity = 350/1030 (33.98%), Postives = 526/1030 (51.07%), Query Frame = 0
Query: 77 VQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQSVSMWVDNL 136
+Q +L + ++G+ +G EKE KL +A+L D K+L ++++ W+ L
Sbjct: 6 LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKL 65
Query: 137 QDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAKKMKDITETL 196
YE DD+LD+ K E + KQ V + P F +K+ K+MK++ E L
Sbjct: 66 NVAAYEVDDILDDC-------KTEAARFKQAVLGRYHP--RTITFCYKVGKRMKEMMEKL 125
Query: 197 DRHYLKASSLGVVGLVGEESTE---TNKNIDLKQFRETTSILNFE--VIGREVEASNILK 256
D + EE + I+ + R T + E V GRE E I+K
Sbjct: 126 D-------------AIAEERRNFHLDERIIERQAARRQTGFVLTEPKVYGREKEEDEIVK 185
Query: 257 LVIDS-SNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIITNIL 316
++I++ S + ++PI+GMGG+GKTTLA+++FN + + HF+ +WVCVS+ F ++
Sbjct: 186 ILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLI 245
Query: 317 EGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKETAG 376
+ I +S+ S G L + L++ + GK YFLVLDDVWNE+ W L+ LK G
Sbjct: 246 KAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IG 305
Query: 377 KSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETVRVE 436
SG I++TTR K+ +IM T + L+ LS + CW LFK A + + +L + E
Sbjct: 306 ASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKE 365
Query: 437 LVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPL 496
+VKK GVPL AK LGG + F+ W + N+ +D+ VL L+LS LP
Sbjct: 366 IVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENS-VLPALRLSYHHLP- 425
Query: 497 LSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLLSRS 556
L L+QCFAYC+ FPKD KE LI +W+A F+ S + M +ED+G +N L RS
Sbjct: 426 LDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLS---KGNMELEDVGNEVWNELYLRS 485
Query: 557 LFQDVFMDKYGRIAYFKMHDLIHDVSCI---------------ISNHQKLELDLPNWSEK 616
FQ++ + K G+ YFKMHDLIHD++ + + + + + N+ +
Sbjct: 486 FFQEIEV-KSGK-TYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDM 545
Query: 617 GARNFRTLVNDNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEII-NYVG 676
+ F +V+ L F+ LRVL L+ + +L +S+G L HLRYL++ N +
Sbjct: 546 MSIGFSEVVSSYSPSLFKR---FVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKIC 605
Query: 677 RLPESITMLYNLQTL---NLGTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQ 736
LP+ + L NLQTL N + LPK KL LR+L + MP + L L+
Sbjct: 606 SLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLK 665
Query: 737 TLDAFVVGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIW 796
TL FVVG +G + EL L NL+G++++++LERVK+ EA A L K +L L W
Sbjct: 666 TLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSW 725
Query: 797 RNVN-YEEEDNNHNDLEVLEGLQPHKNLQSLSIIGFGGQVLP---SNIFVENLVAILLHK 856
N YE E +++VLE L+PH NL+ L II F G LP ++ ++N+V+IL+
Sbjct: 726 DRPNRYESE-----EVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISG 785
Query: 857 CTDCEKLPMLGQLPNLENLYISGMGSVRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQ 916
C +C LP G+LP LE+L + GSV E G + +R FP+L+ L NL+
Sbjct: 786 CENCSCLPPFGELPCLESLELQD-GSVEVEYVEDSGFLTRRR--FPSLRKLHIGGFCNLK 845
Query: 917 LWEKVTNGTAFPRLEGLGINHCSKLMRIPDQFESLQVLRIF-ECEGFIVNEPPNVH-CKY 976
+++ FP LE + I+ C M + S++ L I+ E + ++ N+
Sbjct: 846 GLQRMKGAEQFPVLEEMKISDCP--MFVFPTLSSVKKLEIWGEADAGGLSSISNLSTLTS 905
Query: 977 LQIYNCPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQ 1036
L+I++ N T+ FK L+ +LI S V L +LP
Sbjct: 906 LKIFS--NHTVTSLLEEMFKNLE------------NLIYLS-------VSFLENLKELPT 965
Query: 1037 LLQHFIALKSLTINDFRSLEVLP-EWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLN 1075
L LK L I +LE LP E L L+SL L + +C LK LP E + LT L
Sbjct: 966 SLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLP--EGLQHLTTLT 967
BLAST of Spg005273 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 423.3 bits (1087), Expect = 8.3e-117
Identity = 339/1032 (32.85%), Postives = 513/1032 (49.71%), Query Frame = 0
Query: 77 VQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQSVSMWVDNL 136
+Q +L+ + +++ + +G EKE KL +A+L+D K+L +++ W+ L
Sbjct: 6 LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65
Query: 137 QDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAKKMKDITETL 196
YE DD+L E E +R +Q F+ P FR K+ ++MK+I E L
Sbjct: 66 NSAAYEVDDILGECKNEAIR-------FEQSRLGFYHP--GIINFRHKIGRRMKEIMEKL 125
Query: 197 DRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNF-EVIGREVEASNILKLVID 256
D + + + E RET +L +V GR+ E I+K++I+
Sbjct: 126 DAISEERRKFHFLEKITERQAAA-------ATRETGFVLTEPKVYGRDKEEDEIVKILIN 185
Query: 257 SSN-KHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIITNILEGIF 316
+ N + + PI+GMGG+GKTTLA++IFN E V HF+ +WVCVS+ F +++ I
Sbjct: 186 NVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTII 245
Query: 317 QSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKETAGKSGN 376
++ +S + + + L++ + GK Y LVLDDVWN++ W +L+ L T G G
Sbjct: 246 GNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGA 305
Query: 377 VIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETVRVELVKK 436
I+ TTR KV +IM T +HL+ LS LF A N L + E+VKK
Sbjct: 306 SILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKK 365
Query: 437 IDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLK 496
GVPL AK LGG + F+ W + ++ +D+ +L L+LS LP L L+
Sbjct: 366 CGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESS-ILPALRLSYHHLP-LDLR 425
Query: 497 QCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLLSRSLFQD 556
QCFAYC+ FPKD KE+LI +W+A GF+ S KG E +ED+G +N L RS FQ+
Sbjct: 426 QCFAYCAVFPKDTKMIKENLITLWMAHGFLLS-KGNLE--LEDVGNEVWNELYLRSFFQE 485
Query: 557 VFMDKYGRIAYFKMHDLIHDV-----SCIISNHQKLELDLPNWSEKGARNFRTLVNDNYK 616
+ + YFK+HDLIHD+ S S E+++ ++ + F +V+ +Y
Sbjct: 486 I--EAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVS-SYS 545
Query: 617 VLHTHKLDFLCLRVLVLNPFDKIELL-NSIGMLKHLRYLEI-INYVGRLPESITMLYNLQ 676
K F+ LRVL L+ + K+E L +SIG L HLRYL++ N LPE + L NLQ
Sbjct: 546 PSLLKK--FVSLRVLNLS-YSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQ 605
Query: 677 TLNLGTRVD---LPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFDEGC 736
TL++ LPK KL LRHL + P + L L+TL F+VG +G
Sbjct: 606 TLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKKGY 665
Query: 737 TIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDNNHN 796
+ EL L NL GS+++++LERVK+ +A A L K +L+ L W N ++
Sbjct: 666 QLGELKNL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSWDNDGPNRYES--K 725
Query: 797 DLEVLEGLQPHKNLQSLSIIGFGGQVLPSNI---FVENLVAILLHKCTDCEKLPMLGQLP 856
+++VLE L+PH NL+ L II FGG PS I +E ++++ + C +C LP G+LP
Sbjct: 726 EVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELP 785
Query: 857 NLENLYI-SGMGSVRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQLWEKVTNGTAFPR 916
LENL + +G V + + + + R FP+LK L R+L+ K FP
Sbjct: 786 CLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEEGEEKFPM 845
Query: 917 LEGLGINHCSKLMRIPDQFESLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNGHN 976
LE + I +C + F +L ++ E G N + + L + N
Sbjct: 846 LEEMAILYCPLFV-----FPTLSSVKKLEVHG-------NTNTRGLSSIS---------N 905
Query: 977 MHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHFIALKSLTIND 1036
+ L+I + + + ++ + + LP L ALK L I
Sbjct: 906 LSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIES 965
Query: 1037 FRSLEVLPE-WLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQLQLGRD 1089
SLE PE L LTSL L + YC LK LP E + LT L +L V C +++ D
Sbjct: 966 CDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP--EGLQHLTALTNLGVSGCPEVEKRCD 981
BLAST of Spg005273 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 415.6 bits (1067), Expect = 1.7e-114
Identity = 347/1035 (33.53%), Postives = 505/1035 (48.79%), Query Frame = 0
Query: 77 VQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQSVSMWVDNL 136
+Q +L + ++ + +G + E +L +A+L D K+L+++ + W+ L
Sbjct: 6 IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65
Query: 137 QDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAKKMKDITETL 196
YE DD+LDE K + + Q + P P FR K+ K+M + + L
Sbjct: 66 NAATYEVDDILDE-------YKTKATRFSQSEYGRYHPKVIP--FRHKVGKRMDQVMKKL 125
Query: 197 DRHYLKASSLGVVGLVGEESTETNKNIDLKQ--------FRETTSILNF-EVIGREVEAS 256
+ E KN L + RET S+L +V GR+ E
Sbjct: 126 -----------------KAIAEERKNFHLHEKIVERQAVRRETGSVLTEPQVYGRDKEKD 185
Query: 257 NILKLVIDS-SNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFII 316
I+K++I++ S+ +S++PI+GMGG+GKTTLA+++FN + V HF + +W+CVSE F
Sbjct: 186 EIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDE 245
Query: 317 TNILEGIFQSLTNTS-GGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCL 376
+++ I +S+ G L + L++ + GK Y LVLDDVWNE+ W L+ L
Sbjct: 246 KRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVL 305
Query: 377 KETAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELE 436
K G SG ++ TTR KV +IM T + L+ LS + CW LF A + +N L
Sbjct: 306 K--VGASGASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLV 365
Query: 437 TVRVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSV 496
+ E+VKK GVPL AK LGG + F+ W + N+ +D+ +L L+LS
Sbjct: 366 AIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESS-ILPALRLSY 425
Query: 497 DRLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNL 556
+LP L LKQCFAYC+ FPKD KE LI +W+A GF+ S + M +ED+G+ +
Sbjct: 426 HQLP-LDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLS---KGNMELEDVGDEVWKE 485
Query: 557 LLSRSLFQDVFMDKYGRIAYFKMHDLIHDV-----SCIISNHQKLELDLPNWSEKGARNF 616
L RS FQ++ + K G+ YFKMHDLIHD+ S S+ E++ +++ + F
Sbjct: 486 LYLRSFFQEIEV-KDGK-TYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGF 545
Query: 617 RTLVNDNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEIINYVGR-LPES 676
+V Y + K F+ LRVL L +L +SIG L HLRYL + R LP+
Sbjct: 546 AEVV-FFYTLPPLEK--FISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQ 605
Query: 677 ITMLYNLQTLNLG--TRV-DLPKNLRKLVRLRHLKFFRFR-VQQMPSHMSQLIHLQTLDA 736
+ L NLQTL+L T++ LPK KL LR+L + + MP + L L+TL
Sbjct: 606 LCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQ 665
Query: 737 FVVGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVN 796
FVVG +G + ELG L NL GS+ +S+LERVK+ +A A L K +L L W N
Sbjct: 666 FVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNFG 725
Query: 797 ---YEEEDNNHNDLEVLEGLQPHKNLQSLSIIGFGGQVLP---SNIFVENLVAILLHKCT 856
YE E +++VLE L+PH NL SL I GF G LP ++ ++N+V+IL+
Sbjct: 726 PHIYESE-----EVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFR 785
Query: 857 DCEKLPMLGQLPNLENLYIS-GMGSVRSIGSEFYGNNSN--QRVYFPNLKTLSFNSMRNL 916
+C LP G LP LE+L + G V + +S R+ FP+L+ L +L
Sbjct: 786 NCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSL 845
Query: 917 QLWEKVTNGTAFPRLEGLGINHCSKLMRIPDQFESLQVLRIFECEGFIVNEPPN------ 976
+ K FP LE + I+ C + + +L LRI C + P
Sbjct: 846 KGLLKKEGEEQFPVLEEMIIHEC-PFLTLSSNLRALTSLRI--CYNKVATSFPEEMFKNL 905
Query: 977 VHCKYLQIYNCPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTT 1036
+ KYL I C NL
Sbjct: 906 ANLKYLTISRCNNL---------------------------------------------- 945
Query: 1037 TQLPQLLQHFIALKSLTINDFRSLEVLP-EWLGNLTSLESLELLYCFRLKQLPSKEAMLR 1075
+LP L ALKSL I +LE LP E L L+SL L + +C LK LP E +
Sbjct: 966 KELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLP--EGLQH 945
BLAST of Spg005273 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 412.5 bits (1059), Expect = 1.5e-113
Identity = 328/1015 (32.32%), Postives = 500/1015 (49.26%), Query Frame = 0
Query: 77 VQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQSVSMWVDNL 136
+Q VL + ++ + +G + E +L +A+L D K+L+ + + W+ L
Sbjct: 6 IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65
Query: 137 QDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAKKMKDITETL 196
YE DD+LDE K + + Q + P P FR K+ K+M + + L
Sbjct: 66 NAATYEVDDILDE-------YKTKATRFLQSEYGRYHPKVIP--FRHKVGKRMDQVMKKL 125
Query: 197 DRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNF-EVIGREVEASNILKLVID 256
+ + + + E T RET S+L +V GR+ E I+K++I+
Sbjct: 126 NAIAEERKKFHLQEKIIERQAAT---------RETGSVLTEPQVYGRDKEKDEIVKILIN 185
Query: 257 -SSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIITNILEGIF 316
+S+ +S++PI+GMGG+GKTTL++++FN + V F +W+C+S+ F +++ I
Sbjct: 186 TASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIV 245
Query: 317 QSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKETAGKSGN 376
+S+ S L + L++ + GK YFLVLDDVWNE+ W L+ LK G SG
Sbjct: 246 ESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGA 305
Query: 377 VIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETVRVELVKK 436
++ TTR KV +IM T + L+ LS + CW LF A + +N L + E+VKK
Sbjct: 306 FVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKK 365
Query: 437 IDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLK 496
GVPL AK LGG + F+ W + N+ +D+ +L L+LS LP L L+
Sbjct: 366 CGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESS-ILPALRLSYHHLP-LDLR 425
Query: 497 QCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLLSRSLFQD 556
QCF YC+ FPKD KE+LI W+A GF+ S KG E +ED+G +N L RS FQ+
Sbjct: 426 QCFVYCAVFPKDTKMAKENLIAFWMAHGFLLS-KGNLE--LEDVGNEVWNELYLRSFFQE 485
Query: 557 VFMDKYGRIAYFKMHDLIHDV--SCIISNHQKLELDLPNWSEKG---ARNFRTLVNDNYK 616
+ ++ G+ YFKMHDLIHD+ S +N + N + G + F +V+ +Y
Sbjct: 486 IEVES-GK-TYFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVS-SYS 545
Query: 617 VLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEIIN--YVGRLPESITMLYNLQ 676
K F+ LRVL L + +L +SIG L HLRYL++ + LP+ + L NLQ
Sbjct: 546 PSLLQK--FVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQ 605
Query: 677 TLNL---GTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFDEGC 736
TL+L + LPK KL LR+L + P + L L++L FV+G +G
Sbjct: 606 TLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKG- 665
Query: 737 TIEELGPLKNLK--GSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDNN 796
+LG LKNL GS++++ L+RVK +A A L K +L L W + D
Sbjct: 666 --HQLGELKNLNLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSW------DLDGK 725
Query: 797 HN-DLEVLEGLQPHKNLQSLSIIGFGGQVLP---SNIFVENLVAILLHKCTDCEKLPMLG 856
H D EVLE L+PH NL+ L I GFGG LP + ++N+V+I + C +C LP G
Sbjct: 726 HRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFG 785
Query: 857 QLPNLENLYISGMGSVRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQLWEKVTNGTAF 916
+LP LE+L E + +++ N+ F S+R L +W+ F
Sbjct: 786 ELPCLESL-------------ELHTGSADVEYVEDNVHPGRFPSLRKLVIWD-------F 845
Query: 917 PRLEGLGINHCSKLMRIPDQFESLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNG 976
L+GL M QF L+ + + C F++ P K L++ L
Sbjct: 846 SNLKGL------LKMEGEKQFPVLEEMTFYWCPMFVI--PTLSSVKTLKVIVTDATVLRS 905
Query: 977 -HNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHFIALKSLT 1036
N+ L I +++ + ++ + + + + +LP L ALKSL
Sbjct: 906 ISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLK 951
Query: 1037 INDFRSLEVLP-EWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRC 1072
+LE LP E + LTSL L + C LK LP E + LT L L + +C
Sbjct: 966 FEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLP--EGLQHLTALTTLTITQC 951
BLAST of Spg005273 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 399.1 bits (1024), Expect = 1.7e-109
Identity = 320/1058 (30.25%), Postives = 514/1058 (48.58%), Query Frame = 0
Query: 99 EKELSKLREELLHAEAILRDINRKRLHHQSVSMWVDNLQDIVYEADDLLDELVYEDLRQK 158
E L +L LL A+L D K++ + V WV+ L+D+VY A+D LD++ E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 159 VEIEKTK----QKVRNFFSPSNNPFLFRWKMAKKMKDITETLDRHYLKASSLGVVGLVGE 218
+ E + +++R S + + +++ +T L+R AS ++GL
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLER---LASQRNILGL--- 155
Query: 219 ESTETNKNIDLKQFRETTSILNFEVIGREVEASNILKLVIDSSNK-HLMSIIPIVGMGGI 278
E I ++ T+ + EV GR+ + I++ +I + K + ++++ IVG+GG+
Sbjct: 156 --KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 215
Query: 279 GKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIITNILEGIFQSLTNTSGGWKSKENLVRG 338
GKTTL+++++N + V+ +F +W VSE F + I + +++S+T+ + + L
Sbjct: 216 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 275
Query: 339 LKKEMQGKN--YFLVLDDVWNEEATLWGELKDCLKETAGKSGNVIIVTTRSGKVATIMET 398
LK+ + G + LVLDD+WNE W L+ A G+ I+VTTRS +VA+IM
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCA 335
Query: 399 TSSHHLNKLSDDQCWSLFKDSANAN-GLLMNQELETVRVELVKKIDGVPLVAKVLGGAVT 458
H+L LSD CWSLF + N +N+E+ + +V K G+PL K LGG +
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 459 FEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFN 518
FEG W + + ++ DK +L +L++S LP LK+CFAYCS FPK H F
Sbjct: 396 FEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 455
Query: 519 KESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLLSRSLFQDVFMDKYGRIAYFKMHD 578
K+ ++ +W+A+GF+Q + +E++G YF+ L SRSL Q + Y MHD
Sbjct: 456 KDKVVLLWMAEGFLQQTRSSKN--LEELGNEYFSELESRSLLQKT------KTRYI-MHD 515
Query: 579 LIHDVSCIISNH--QKLELDLPNWSEKGARNFRTLVNDNYKVLHTHKLDFLCLR-VLVLN 638
I++++ S K E D + + + DNY ++F LR V L
Sbjct: 516 FINELAQFASGEFSSKFE-DGCKLQVSERTRYLSYLRDNY----AEPMEFEALREVKFLR 575
Query: 639 PFDKIELLNS--------------IGMLKHLRYLEIINY--------------------- 698
F + L NS + L LR L + +Y
Sbjct: 576 TFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL 635
Query: 699 ----VGRLPESITMLYNLQTLNL---GTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMS 758
+ +LP+S+ +YNLQTL L + +LP ++ L+ LR+L +++QMP
Sbjct: 636 SRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFG 695
Query: 759 QLIHLQTLDAFVVGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLR 818
+L LQTL F V +G I ELG L +L G L + L+RV A+A A L K+ LR
Sbjct: 696 RLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLR 755
Query: 819 RLDFIWRNVNYEEEDNNH-----NDLEVLEGLQPHKNLQSLSIIGFGGQVLP---SNIFV 878
+DF+WR + E+N + N+ EV E L+PH++++ L+I + G+ P S+
Sbjct: 756 EIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSF 815
Query: 879 ENLVAILLHKCTDCEKLPMLGQLPNLENLYISGMGSVRSIGSEFYGNNSNQRVY----FP 938
+V I L +C C LP LGQLP L+ L+ISGM ++SIG +FY ++ R F
Sbjct: 816 SRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFR 875
Query: 939 NLKTLSFNSMRNLQLW--EKVTNGTAFPRLEGLGINHCSKLM-RIPDQFESLQVLRIFEC 998
+L+TL F+++ + Q W +VT G FP L+ L I C +L +P SL L I++C
Sbjct: 876 SLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKC 935
Query: 999 EGFIVNEPPNVHCKYLQIYNCPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVIN 1058
G + +P H+ ++++ L+
Sbjct: 936 -GLLDFQP-------------------DHHEYSYRNLQ---------------------T 995
Query: 1059 LCLVGGLVTTTQLPQLLQHFIALKSLTIN---DFRSLEVLPEWLGNLTSLESLELLYCFR 1083
L + T + P L HF L L ++ SLE+ E L +L +L + C
Sbjct: 996 LSIKSSCDTLVKFP--LNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQN 1024
BLAST of Spg005273 vs. ExPASy TrEMBL
Match:
A0A6J1CUL8 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111014734 PE=4 SV=1)
HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 675/1033 (65.34%), Postives = 792/1033 (76.67%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVLKKVLKLAAEQIGVAW L+KELSKLR+ LL AE IL DINR++L H+
Sbjct: 1 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +WV++LQ +VY+ADDLLD+LVYEDLRQKVE+EK KVRNF SPSNNPFLFR+KMAK
Sbjct: 61 SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKI-NKVRNFLSPSNNPFLFRFKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
+MK I+ETL HY +AS V+G VGEESTET L+Q RETTSILNF+VIGRE+E
Sbjct: 121 RMKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVL 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
I+KLVIDSS++H MSIIPIVGMGG+GKTTLAK++FNHEL+K HFD T+WVCVS+PFII
Sbjct: 181 KIMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIV 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
NILEGIFQSLTNTS G KE L L+KEMQGK YFLVLDDVWNEE LW EL+DCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
A KSGN IIVTTRS VATIM+T SH L KLSDD+CWSLFK+SANANGLLMN ELE +
Sbjct: 301 IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 487
R +VK+I G+PLVAKVLGG FEGN+ERWVT V+ +V+NI D+KDFVLS LKLSVD
Sbjct: 361 REVVVKRIGGIPLVAKVLGGTAKFEGNYERWVTTVESIVRNILNDEKDFVLSTLKLSVDS 420
Query: 488 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLL 547
LPLLSLKQCFA+CSNFPKD F KE+LI+MWIAQGF+Q ++G+N ++ E+IGEGYFN LL
Sbjct: 421 LPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFNFLL 480
Query: 548 SRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKLELDLPNWSEKGARNFRTLVNDN 607
SRSLFQDV D G I +FKMHDLIHDV+CI+SN QKLELD NWSEKG R RTLV +N
Sbjct: 481 SRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRALRTLVINN 540
Query: 608 YKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEII-NYVGRLPESITMLYNL 667
++H D + LRVLVLN L +SIGMLKHLRYL+II + + RLPESITMLYNL
Sbjct: 541 GAIIHNKLEDSVYLRVLVLNSDFIKNLPDSIGMLKHLRYLDIIGSRIRRLPESITMLYNL 600
Query: 668 QTLNLGTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFDEGCTI 727
Q L L TR+DLPKNLRKLV LRHL FF +++MP H+SQLIHLQTL ++VGF++G I
Sbjct: 601 QILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYIVGFEKGRKI 660
Query: 728 EELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEED-NNHND 787
EELGPL +LKGSL L NLERVKSK EAI AKL+EK +L L W + + EE NN ND
Sbjct: 661 EELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTLHWNSSSCGEEGYNNRND 720
Query: 788 LEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQLPNLEN 847
EVLEGLQPH+NLQSLSI FGG LP+ +FVENLVAI+L +C CE LPMLGQLPNLE
Sbjct: 721 SEVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAIVLQECNKCEMLPMLGQLPNLEE 780
Query: 848 LYISGMGSVRSIGSEFYGNNSNQR--VYFPNLKTLSFNSMRNLQLWEKVTNGTAFPRLEG 907
L +S MGSVR IGSEFYGNNSN R ++FP L+ LSF+ M L+ WE+++NG FP LE
Sbjct: 781 LRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFSHMEKLEQWEELSNGINFPHLET 840
Query: 908 LGINHCSKLMRIPD-QFESLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNGHNMH 967
L + +CSKL +PD +F SL+ LRI CE C +++I CP L LN HNM
Sbjct: 841 LSMFYCSKLTSVPDHEFVSLRTLRIVGCEELSKLPSKLECCNFMKISGCPKLRLNMHNMC 900
Query: 968 NFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHFIALKSLTINDFR 1027
N EL+I G ++ +D L+ QSSV N+ L L TQLPQ LQH LKSL I F
Sbjct: 901 NLNELEINGPMEEYDFS-FLMHQSSVTNIFLTDSLRNATQLPQQLQHLTTLKSLAIEYFN 960
Query: 1028 SLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQLQLGRDHPE 1087
+E LPEWL L +LE++ + C LK+LPS++AMLRLTKL HL VR C L LG+ E
Sbjct: 961 GVEALPEWLEKLANLETMGVFECKNLKRLPSQQAMLRLTKLKHLRVRGCPLLPLGQGDLE 1020
Query: 1088 GAKLSHFLISNEN 1096
AKLSH I+ +N
Sbjct: 1021 RAKLSHLPITVDN 1027
BLAST of Spg005273 vs. ExPASy TrEMBL
Match:
A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)
HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 636/1027 (61.93%), Postives = 755/1027 (73.52%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVL+K++K+AA+QIG+AWGLEKELSKL + LL AE IL DINR++LHH
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +W+++LQD+V+EA DLLDELVYEDLR+KVEI K KVR S S N F FR KMAK
Sbjct: 61 SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEIGK-MNKVRGSISRSYNLFSFRRKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
KMKD+TETL +HY +AS L GLVG+ES ETN+ I LKQ RETTS LNFEV+GRE+E S
Sbjct: 121 KMKDVTETLYKHYCEASPL---GLVGDESIETNQ-IVLKQIRETTSNLNFEVVGREIEVS 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
NI+KLVIDSSN+H SIIPIVGMGG+GKTTLA++IFNHE++KGHFD T+W+CVSEPFII
Sbjct: 181 NIVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIII 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
NILEGIFQ LT TS G SKE LV+ L++EM GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 NILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQ 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
KSGN IIVTTRS +VAT +ET SSHHL KLSDDQ W LFK SANANGL MN +L+ +
Sbjct: 301 ITEKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDII 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 487
R LVKKI GVPLVAKVLGGA+ FEG++E WV +VK VV+NISKDDKDFVLSILKLSVD
Sbjct: 361 REILVKKIGGVPLVAKVLGGAIKFEGDYETWVKKVKSVVRNISKDDKDFVLSILKLSVDC 420
Query: 488 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLL 547
LPLLSLKQCFAYCSNF KD +F+KE+LI+MWIAQGF+Q +G NE EDIGE + N LL
Sbjct: 421 LPLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNFLL 480
Query: 548 SRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQK-LELDLPNWSEKGARNFRTLVND 607
SRSLFQDV D R+ +FKMHDLIHD++C ISNH K LEL NWS K AR RTL+ +
Sbjct: 481 SRSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLICN 540
Query: 608 NYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEIINYVGR-LPESITMLYN 667
N +V++ +D + LRVLVLN LL+SIG LKHLRYL+I +Y R LPESI LYN
Sbjct: 541 N-QVIYNELMDCVSLRVLVLNSAKTDNLLDSIGKLKHLRYLDISHYSIRVLPESIAKLYN 600
Query: 668 LQTLNLGTRVDLPKNLRKLVRLRHLKFFR-FRVQQMPSHMSQLIHLQTLDAFVVGFDEGC 727
LQTL +G LPKNLRK+V LRHLKF+ + QMPSHMS LIHLQTL F GF+ GC
Sbjct: 601 LQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAGFENGC 660
Query: 728 TIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDNNHN 787
IEELGPLKNLKG L LSNLERVKSK EAI AKL+EK++L L F W ++++E D +
Sbjct: 661 KIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDW-SLDFERND---D 720
Query: 788 DLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQLPNLE 847
D EVLEGLQPHKNLQSL+I+ FGGQVLP IFVENL I L CE LPMLGQLPNL
Sbjct: 721 DSEVLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAVIRLCGGKKCEMLPMLGQLPNLV 780
Query: 848 NLYISGMGSVRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQLW-EKVTNGTAFPRLEG 907
L IS M SVR IGSEFYGN S F L+ F + NL+ W E + +F L+
Sbjct: 781 ELEISFMDSVRCIGSEFYGNGS-----FSKLRKFDFIGLHNLEQWTEAASISNSFGSLQT 840
Query: 908 LGINHCSKLMRIPDQFE---SLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLTLNGHN 967
L + C L ++P+ E SL L + +C +N + L I+ L L +
Sbjct: 841 LNVIGCGNLKKLPNGLEFCSSLHDLTVRDCPKLALNVQNMHNLSNLFIHGFKELPLGLSH 900
Query: 968 MHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTTTQLPQLLQHFIALKSLTIND 1027
+ N KE+++VG +Q +D P L Q S+I L L T+LPQ ++ AL++LTI +
Sbjct: 901 LTNLKEMEVVGCMQDYDFSPFLSLQ-SLIKLHLNDVSRICTELPQHFENLTALETLTIVN 960
Query: 1028 FRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQLQLGRDH 1087
F ++E LPEWL NL SLE+L L C LK+LPS+EAMLRLTKL HL V RC QL D
Sbjct: 961 FDAIEALPEWLENLASLETLNLEDCKNLKRLPSREAMLRLTKLEHLKVFRCPQLPGRGDL 1011
BLAST of Spg005273 vs. ExPASy TrEMBL
Match:
A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)
HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 622/1023 (60.80%), Postives = 745/1023 (72.83%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVL+K++K+AA+QIG+A GLEKELS L + L AE ILRDINRK+ H
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSHSD 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +WV++LQD+V+EADDLLDELVYE LR+KVEI K KVR+ FS S+N FLFR KMAK
Sbjct: 61 SVRLWVEDLQDVVHEADDLLDELVYEHLRRKVEIGKI-NKVRDSFSTSHNVFLFRLKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
K+KDIT+ L +HY +AS L GLVG+ESTE N+ I L Q R+TTS LNFEV+GRE E S
Sbjct: 121 KIKDITQILHKHYCEASPL---GLVGQESTEANQ-IVLSQIRDTTSNLNFEVVGREDEVS 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
NI+KLVIDSSN+H MSI+PIVGMGG+GKTT A+ IFNH+ +KGHFDN +WVCVSEPFII
Sbjct: 181 NIVKLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIII 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
NILEGIFQSLT TS G K L+ ++KEM GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 NILEGIFQSLTKTSSGLNHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQ 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
AGKSGN IIVTTRS +VA I+ T SH L KLSDDQCW LFK+SANAN L MN +LE +
Sbjct: 301 IAGKSGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKESANANRLSMNPKLEII 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDR 487
R LVKKI GVPLVAKVLGGA FEG++ERWVT+V+ VV+NIS DDKDFV SILKLSVD
Sbjct: 361 RDMLVKKIGGVPLVAKVLGGATKFEGDYERWVTKVESVVRNISNDDKDFVSSILKLSVDC 420
Query: 488 LPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFV--QSQKGENEMMMEDIGEGYFNL 547
LPLLSLKQCFAYCSNF KD L++KE+LIR+WIAQGF+ Q Q+G+NEM EDIGE +FN
Sbjct: 421 LPLLSLKQCFAYCSNFAKDCLYDKENLIRIWIAQGFIQPQDQEGDNEMTEEDIGEWHFNF 480
Query: 548 LLSRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKL--ELDLPNWSEKGARNFRTL 607
LLSRSLFQDV MDK R YF MHDLIHD++C ISNHQKL EL N S K AR RTL
Sbjct: 481 LLSRSLFQDVTMDKSERGIYFIMHDLIHDIACAISNHQKLLHELSSSNRSRKSARKLRTL 540
Query: 608 VNDNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEII--NYVGRLPESIT 667
+ +N +V++ +D + LRVLVL+ LL SIG LKHLRYL I NYV LPESI
Sbjct: 541 ICNN-QVIYNELVDCVSLRVLVLHCEKTGNLLESIGKLKHLRYLSIANGNYVDVLPESIV 600
Query: 668 MLYNLQTLNLGTRVDLPKNLRKLVRLRHLKF-FRFRVQQMPSHMSQLIHLQTLDAFVVGF 727
MLYNLQTL L LPKN RK+V LRHL+F + +QMPSHMS+LIHLQTL FV GF
Sbjct: 601 MLYNLQTLKLRHMKSLPKNSRKMVSLRHLEFELKSDFRQMPSHMSELIHLQTLSRFVAGF 660
Query: 728 DEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEED 787
+ GC IEELGPLKNLKG L LS LERV+SK E+I AKL+EK++L+ L F W + +E ++
Sbjct: 661 ENGCKIEELGPLKNLKGKLQLSCLERVESKEESIVAKLVEKKNLQELIFEWSS-RFEGKN 720
Query: 788 NNHNDLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQL 847
+ +++LEVLEGLQPHKNLQSL++IGFGGQVL IFVENL I L C +CE+LPMLG+L
Sbjct: 721 SRYSELEVLEGLQPHKNLQSLTVIGFGGQVLGDAIFVENLTVIRLSNCKNCERLPMLGRL 780
Query: 848 PNLENLYISGMGSVRSIGSEFYGNNSNQRVY-FPNLKTLSFNSMRNLQLW-EKVTNGTAF 907
PNL L I+ M S+R IGSEFYGN SN + + F L+ L+F RNL+ W + +F
Sbjct: 781 PNLVELNINSMDSLRCIGSEFYGNGSNNQGHSFSKLRKLTFIWCRNLEQWKDAAVVSNSF 840
Query: 908 PRLEGLGINHCSKLMRIPDQFE---SLQVLRIFECEGFIVNEPPNVHCKYLQIYNCPNLT 967
L+ L I C L ++P+ E SL+ L + C +N + L+I L
Sbjct: 841 GSLQTLSIYGCGNLEKLPNGLEGCSSLRDLTVAYCPKLSLNVQNCQNLSNLRIDGLKKLP 900
Query: 968 LNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLV-TTTQLPQLLQHFIALK 1027
L ++ N KE+K VG +Q +D P + QS +I L L TQLP LQH ALK
Sbjct: 901 LGLGHLTNLKEMKFVGCMQDYDFTPFVSLQSLIIKLELRDECSGNCTQLPPHLQHLTALK 960
Query: 1028 SLTINDFRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHLYVRRCRQL 1078
L+I +F +EV PEWL NLTSLE L C L++LPS EA+ RLTKL L +C L
Sbjct: 961 QLSIGNFGGIEVPPEWLNNLTSLEMLSFEGCENLRRLPSTEAIRRLTKLELLSAIKCPLL 1016
BLAST of Spg005273 vs. ExPASy TrEMBL
Match:
A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)
HSP 1 Score: 1018.1 bits (2631), Expect = 2.8e-293
Identity = 571/1042 (54.80%), Postives = 713/1042 (68.43%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAEFLWTFAVQEVLKK++ AEQI +AWGLEKELS L++ LL A+ IL DIN K+ HH
Sbjct: 1 MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
SV +WV+ L DI+YEADDLLDE+VYE +RQ VE +KVR+ SPS N FLF KMAK
Sbjct: 61 SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMAK 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 247
KMK IT+TL HY +AS L GLVG+EST T L Q RETTSIL+FEV GRE E
Sbjct: 121 KMKKITKTLYEHYCEASPL---GLVGDEST-TESEAALNQIRETTSILDFEVEGREAEVL 180
Query: 248 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 307
ILKLVIDS+++ +S+I IVGMGG+GKTTLAK++FNH+ +KGHFD T+WVCVS+PFI+
Sbjct: 181 EILKLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVM 240
Query: 308 NILEGIFQSLTNTSGGWKSKENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKE 367
ILE IFQ LTNTS G S+E L+ L++EMQGK YFLVLDDVW++E LW EL LK
Sbjct: 241 KILEAIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKY 300
Query: 368 TAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELETV 427
AGKSGN I+VTTRS +VAT+++T +HL KLSDD CW+L K SANAN L MN +LE
Sbjct: 301 IAGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENT 360
Query: 428 RVELVKKIDGVPLVAKVLGGAVTF-EGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVD 487
+ LV+KI GVPL+AKVLGGAV F EG E W+ +++ +NIS +DKDFVLSILKLSV+
Sbjct: 361 KNILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKDFVLSILKLSVE 420
Query: 488 RLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLL 547
LP +LKQCFAYCSNFP+D+ F+K+ I+MWIA+GF+Q ++ + ME+IGE Y N L
Sbjct: 421 SLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEYLNFL 480
Query: 548 LSRSLFQDVFMDKY-GRIAYFKMHDLIHDVSCIISNHQKLELDLPNWSEKGARNFRTLVN 607
LSRSLF+D KY GRI FK+HDL+HD++C ISNH K++ + +W+ K R RTL+
Sbjct: 481 LSRSLFEDAI--KYDGRIVTFKIHDLMHDIACAISNHHKMDSNPISWNGKSTRKLRTLIC 540
Query: 608 DNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEI--INYVGRLPESITML 667
+N + H + D +CLRVLVL FD L + L HLRYL+I N L +SI L
Sbjct: 541 ENEEAFHKIQTDIICLRVLVLKWFDTNTLSTIMDKLIHLRYLDISNCNINKLLRDSICAL 600
Query: 668 YNLQTLNLG-TRVDLPKNLRKLVRLRHLKFFR-FRVQQMPSHMSQLIHLQTLDAFVVGFD 727
YNLQTL LG DLPKNLR LV LRHL+F + F + QMPSHM +IHLQTL FVVG +
Sbjct: 601 YNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVGLE 660
Query: 728 EGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIW-----RNVNY 787
+GC I+ELGPLK+LKG+LTL NL+ V++K EA+ AKL+EK+ LR L F W Y
Sbjct: 661 KGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYDRGEY 720
Query: 788 EEEDNNHNDLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPM 847
+E+DN +VLEGLQPHKN+QSL I GF G+VL +NIFVENLV I L C CE LPM
Sbjct: 721 DEDDNK----QVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPM 780
Query: 848 LGQLPNLENLYISGMGSVRSIGSEFYGNNSNQR--VYFPNLKTLSFNSMRNLQLWEKVT- 907
LGQLPNL+ L I M SVRSIGSEFYG + N R FP L ++ LQ W++ T
Sbjct: 781 LGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEATV 840
Query: 908 -NGTAFPRLEGLGINHCSKLMRIPDQFE---SLQVLRIFECEGFIVNEPPNVHCKYLQIY 967
F L+ L ++ C +L ++P E S++ L I C ++N + +L I
Sbjct: 841 FASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIR 900
Query: 968 NCPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLV---GGLVTTTQLPQL 1027
L + N K+L+I G +Q+++ P + S ++ L L TTQLPQ
Sbjct: 901 GLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLPQQ 960
Query: 1028 LQHFIALKSLTINDFRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKLNHL 1087
LQH LK L I DF +EVLPEWLGNLT L +L L C LK+LPS+EA+ RLTKL+ L
Sbjct: 961 LQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLDDL 1020
Query: 1088 YVRRCRQLQLGRDHPEGAKLSH 1089
+ C +L LG E AKLSH
Sbjct: 1021 VIDGCPKLLLGEGDQERAKLSH 1032
BLAST of Spg005273 vs. ExPASy TrEMBL
Match:
A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)
HSP 1 Score: 983.4 bits (2541), Expect = 7.7e-283
Identity = 564/1045 (53.97%), Postives = 722/1045 (69.09%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLH-H 127
MAE + TF VQEVLK+++K AEQI VAW LE E+S L+++L A+ IL DINRK+ H
Sbjct: 1 MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
Query: 128 QSVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMA 187
SV WV+ L+DIV+EADDLLDELVYE LR+ VE + KV + S S N FLFR KMA
Sbjct: 61 NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
Query: 188 KKMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEA 247
KK+K+IT+TL++HY AS+ G+VG+ T T + L Q RETTSIL+F+V GRE E
Sbjct: 121 KKIKNITDTLNQHYCAASAFGLVGV----ETVTEIELALNQIRETTSILDFQVEGREAEV 180
Query: 248 SNILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFII 307
+LKL IDS+N+H MS+I IVGMGG+GKTTLAK+IFNH ++GHFD T+WVCVS+PFI+
Sbjct: 181 LELLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIV 240
Query: 308 TNILEGIFQSLTNTSGGWKS-KENLVRGLKKEMQGKNYFLVLDDVWNEEATLWGELKDCL 367
T ILE IFQ LT T G +S KE L+ L+KEMQ KNYFLVLDDVW+ E LW EL+ CL
Sbjct: 241 TKILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCL 300
Query: 368 KETAGKSGNVIIVTTRSGKVATIMETTSSHHLNKLSDDQCWSLFKDSANANGLLMNQELE 427
K AGK GN I+VTTR+ +VAT++E S + L KLS+DQCW+LFK+SANAN L MN +LE
Sbjct: 301 KHIAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLE 360
Query: 428 TVRVELVKKIDGVPLVAKVLGGAVTF-------EGNFERWVTEVKRVVKNISKDDKDFVL 487
++ ELV+K+ GVPLVAKVLGGAV F E + W+T+V+ +V+NIS +DKDFVL
Sbjct: 361 IMKKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVL 420
Query: 488 SILKLSVDRLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKG-ENEMMMED 547
SILKLSVD LP LKQC AYCSNF +D+ F K+ LI+MWIAQGF+Q +G + ++MED
Sbjct: 421 SILKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMED 480
Query: 548 IGEGYFNLLLSRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKLELDLPNWSEKGA 607
IGE YFN LLSRS+FQDV D RI FKMHDL+HD++C IS+HQ +E + N S K
Sbjct: 481 IGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSV 540
Query: 608 RNFRTLVNDNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEI----INYV 667
R RTL+ ++ + + ++ D +CLRVL + +L I L HLRYL+I IN +
Sbjct: 541 RKLRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKL 600
Query: 668 GRLPESITMLYNLQTLNLGTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTL 727
L ES+++LYNLQTL LG + LPKNLRKLV LRHL+F F MPS M LIHLQ+L
Sbjct: 601 --LLESLSLLYNLQTLKLG-QSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSL 660
Query: 728 DAFVVGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRN 787
F+VGF++GC IEELGPLKNLKG LTL+NL RV++K EA+ AKL+EK++LR L+ +
Sbjct: 661 SGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFE 720
Query: 788 VNYEEEDNNHNDLEVLEGLQPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEK 847
+ ED+ ++VLEGLQPHKNLQSL I+GF G+VLP+ IFVENLV I L CE
Sbjct: 721 TDKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEV 780
Query: 848 LPMLGQLPNLENLYISGMGSVRSIGSEFYGNNSNQR--VYFPNLKTLSFNSMRNLQLWEK 907
LPMLGQLPNL+ L I M SVRSIG+EFYG +S+ + V FP LK LS M NL+ W++
Sbjct: 781 LPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDE 840
Query: 908 VT---NGTAFPRLEGLGINHCSKLMRIPDQFE---SLQVLRIFECEGFIVNEPPNVHCKY 967
T F L+ + I C+ L ++P E SL+ L I C ++N N+H Y
Sbjct: 841 ATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLN-VQNLHKLY 900
Query: 968 -LQIYNCPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVINLCLVGGLVTT-TQL 1027
L+I L + KELKI G +Q+++ + S ++ L L G + TQL
Sbjct: 901 HLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQL 960
Query: 1028 PQLLQHFIALKSLTINDFRSLEVLPEWLGNLTSLESLELLYCFRLKQLPSKEAMLRLTKL 1087
PQ LQH L+ L I F +E LPEW+GNL SL++L+ YCF+LK+LPS+EA+LRLTKL
Sbjct: 961 PQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKL 1020
Query: 1088 NHLYVRRCRQLQLGRDHPEGAKLSH 1089
+L + C +L +G E AKLSH
Sbjct: 1021 ENLDIFECPKLLVGEGDQERAKLSH 1036
BLAST of Spg005273 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 399.1 bits (1024), Expect = 1.2e-110
Identity = 320/1058 (30.25%), Postives = 514/1058 (48.58%), Query Frame = 0
Query: 99 EKELSKLREELLHAEAILRDINRKRLHHQSVSMWVDNLQDIVYEADDLLDELVYEDLRQK 158
E L +L LL A+L D K++ + V WV+ L+D+VY A+D LD++ E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 159 VEIEKTK----QKVRNFFSPSNNPFLFRWKMAKKMKDITETLDRHYLKASSLGVVGLVGE 218
+ E + +++R S + + +++ +T L+R AS ++GL
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLER---LASQRNILGL--- 155
Query: 219 ESTETNKNIDLKQFRETTSILNFEVIGREVEASNILKLVIDSSNK-HLMSIIPIVGMGGI 278
E I ++ T+ + EV GR+ + I++ +I + K + ++++ IVG+GG+
Sbjct: 156 --KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 215
Query: 279 GKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIITNILEGIFQSLTNTSGGWKSKENLVRG 338
GKTTL+++++N + V+ +F +W VSE F + I + +++S+T+ + + L
Sbjct: 216 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 275
Query: 339 LKKEMQGKN--YFLVLDDVWNEEATLWGELKDCLKETAGKSGNVIIVTTRSGKVATIMET 398
LK+ + G + LVLDD+WNE W L+ A G+ I+VTTRS +VA+IM
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCA 335
Query: 399 TSSHHLNKLSDDQCWSLFKDSANAN-GLLMNQELETVRVELVKKIDGVPLVAKVLGGAVT 458
H+L LSD CWSLF + N +N+E+ + +V K G+PL K LGG +
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 459 FEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFN 518
FEG W + + ++ DK +L +L++S LP LK+CFAYCS FPK H F
Sbjct: 396 FEGKVIEWERVLSSRIWDLPA-DKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 455
Query: 519 KESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLLSRSLFQDVFMDKYGRIAYFKMHD 578
K+ ++ +W+A+GF+Q + +E++G YF+ L SRSL Q + Y MHD
Sbjct: 456 KDKVVLLWMAEGFLQQTRSSKN--LEELGNEYFSELESRSLLQKT------KTRYI-MHD 515
Query: 579 LIHDVSCIISNH--QKLELDLPNWSEKGARNFRTLVNDNYKVLHTHKLDFLCLR-VLVLN 638
I++++ S K E D + + + DNY ++F LR V L
Sbjct: 516 FINELAQFASGEFSSKFE-DGCKLQVSERTRYLSYLRDNY----AEPMEFEALREVKFLR 575
Query: 639 PFDKIELLNS--------------IGMLKHLRYLEIINY--------------------- 698
F + L NS + L LR L + +Y
Sbjct: 576 TFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL 635
Query: 699 ----VGRLPESITMLYNLQTLNL---GTRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMS 758
+ +LP+S+ +YNLQTL L + +LP ++ L+ LR+L +++QMP
Sbjct: 636 SRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFG 695
Query: 759 QLIHLQTLDAFVVGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLR 818
+L LQTL F V +G I ELG L +L G L + L+RV A+A A L K+ LR
Sbjct: 696 RLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLR 755
Query: 819 RLDFIWRNVNYEEEDNNH-----NDLEVLEGLQPHKNLQSLSIIGFGGQVLP---SNIFV 878
+DF+WR + E+N + N+ EV E L+PH++++ L+I + G+ P S+
Sbjct: 756 EIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSF 815
Query: 879 ENLVAILLHKCTDCEKLPMLGQLPNLENLYISGMGSVRSIGSEFYGNNSNQRVY----FP 938
+V I L +C C LP LGQLP L+ L+ISGM ++SIG +FY ++ R F
Sbjct: 816 SRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFR 875
Query: 939 NLKTLSFNSMRNLQLW--EKVTNGTAFPRLEGLGINHCSKLM-RIPDQFESLQVLRIFEC 998
+L+TL F+++ + Q W +VT G FP L+ L I C +L +P SL L I++C
Sbjct: 876 SLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKC 935
Query: 999 EGFIVNEPPNVHCKYLQIYNCPNLTLNGHNMHNFKELKIVGDIQHFDVDPDLIQQSSVIN 1058
G + +P H+ ++++ L+
Sbjct: 936 -GLLDFQP-------------------DHHEYSYRNLQ---------------------T 995
Query: 1059 LCLVGGLVTTTQLPQLLQHFIALKSLTIN---DFRSLEVLPEWLGNLTSLESLELLYCFR 1083
L + T + P L HF L L ++ SLE+ E L +L +L + C
Sbjct: 996 LSIKSSCDTLVKFP--LNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQN 1024
BLAST of Spg005273 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 315.8 bits (808), Expect = 1.3e-85
Identity = 260/895 (29.05%), Postives = 435/895 (48.60%), Query Frame = 0
Query: 102 LSKLREELLHAEAILRDINRKRLHHQSVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEI 161
L +L+ L+ A +L D +++ H + V W+ ++D ++A+D+LDEL E LR++V
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 162 EK--TKQKVRNFFSPSNNPFLFRWKMAKKMKDITETLDRHYLKASSLGVVGLVGEESTET 221
E +N + + K+ KM+ + L+ H + V+GL +E +ET
Sbjct: 96 EAGGLGGLFQNLMAGRE---AIQKKIEPKMEKVVRLLEHH---VKHIEVIGL--KEYSET 155
Query: 222 NKNIDLKQFRETT-----SILNFEVIGREVEASNILKLVI--DSSNKHLMSIIPIVGMGG 281
+ Q+R+ + + ++GR + ++ L++ D + ++I +VGM G
Sbjct: 156 RE----PQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPG 215
Query: 282 IGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIITNILEGIFQSLTNTSGGWKSKENLVR 341
+GKTTL +++FN V HF+ +W+ F + + + + Q +T+++ + +L
Sbjct: 216 VGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQI 275
Query: 342 GLKKEMQGKNYFLVLDDVWNEEATLWGELKDCLKETAGKSGNVIIVTTRSGKVATIMETT 401
LKK + GK + LVLDD W+E + W + + T + G+ I++TTRS V+T+ +
Sbjct: 276 QLKKTLSGKRFLLVLDDFWSESDSEWESFQ--VAFTDAEEGSKIVLTTRSEIVSTVAKAE 335
Query: 402 SSHHLNKLSDDQCWSLFKDSANANGLL--MNQELETVRVELVKKIDGVPLVAKVLGGAVT 461
+ + +++++CW L A N + +NQELE + + ++ G+PL A+ + +
Sbjct: 336 KIYQMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLR 395
Query: 462 FEGNFERWVTEVKRVVKNISKDDKDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFN 521
+ N + W V KN S +L +LKLS D LP LK+CFA CS FPK H+F+
Sbjct: 396 SKPNPDDWYA----VSKNFSSYTNS-ILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFD 455
Query: 522 KESLIRMWIAQGFVQSQKGENEMMMEDIGEGYFNLLLSRSLFQ--DVFMDKYGRIAYFKM 581
+E L+ +W+A + + +EDIG Y L+++S FQ D+ M F M
Sbjct: 456 REELVLLWMAIDLLYQPRSSRR--LEDIGNDYLGDLVAQSFFQRLDITMTS------FVM 515
Query: 582 HDLIHDVSCIISNHQKLELDLPNWSE------------------------KGARNFRTLV 641
HDL++D++ +S L+ N E GA RT++
Sbjct: 516 HDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTIL 575
Query: 642 NDN-----------YKVLHTHKLDFLCLRVLVLNPFDKIELLNSIGMLKHLRYLEIIN-Y 701
N KVL+ LR+L L+ + L S+ LK LRYL++ +
Sbjct: 576 PFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTK 635
Query: 702 VGRLPESITMLYNLQTLNLGTRVD---LPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIH 761
+ LPE + L NLQTL L D LPK++ +L+ LR L + +MP + +L
Sbjct: 636 IKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRS 695
Query: 762 LQTLDAFVVGFDEGCTIEELGPLKNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDF 821
LQ L FV+G G + EL L +L+G+L +S L+ V +EA +A L K L L
Sbjct: 696 LQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLIL 755
Query: 822 IWRNVNYEEEDNNHNDL-----EVLEGLQPHKNLQSLSIIGFGGQVLP-----SNIFVEN 881
W + N L EVL L+PH +L++ I + G P S+ F
Sbjct: 756 KWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFF--G 815
Query: 882 LVAILLHKCTDCEKLPMLGQLPNLENLYISGMGSVRSIGSE-FYGNNSNQRVYFPNLKTL 931
+ ++ L C C LP +GQLP+L+ L I ++ +G + F+G N+++ V F +L+ L
Sbjct: 816 ITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQIL 875
BLAST of Spg005273 vs. TAIR 10
Match:
AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 223.4 bits (568), Expect = 8.9e-58
Identity = 234/918 (25.49%), Postives = 429/918 (46.73%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAE + +F V+ K+ +L + + G+++++ L+ +L +++L+D + K+ +
Sbjct: 1 MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLFRWKMAK 187
V ++++++DIVY+ADD+++ + +LR K + K + + F + R K A
Sbjct: 61 RVRNFLEDVKDIVYDADDIIESFLLNELRGKEKGIKKQVRTLACF------LVDRRKFAS 120
Query: 188 KMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRETTS-ILNFEVIGREVEA 247
++ IT+ + + SLG+ + + ++ R+T S +++G +
Sbjct: 121 DIEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSV 180
Query: 248 SNILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFII 307
++ ++++ + + ++ + GMGGIGKTTLA+ +F+H++V+ HFD WVCVS+ F
Sbjct: 181 EELVDHLVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTR 240
Query: 308 TNILEGIFQSLTNTSGG-WKSKENLVRG-LKKEMQGKNYFLVLDDVWNEEATLWGELKDC 367
++ + I Q L G + E ++G L + ++ Y LVLDDVW EE W +K
Sbjct: 241 KDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAV 300
Query: 368 LKETAGKSGNVIIVTTRSGKVATIMETTS-SHHLNKLSDDQCWSLFKDSANANGLLMNQE 427
K G +++T+R+ + + T + L+ +Q W LF+ ++ +
Sbjct: 301 FPH---KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKTEFK 360
Query: 428 L-ETVRVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNI------SKDDKDF 487
+ E + E+V G+PL KVLGG + + W +V +I S D+ +
Sbjct: 361 VDEAMGKEMVTYCGGLPLAVKVLGGLLAKKHTVLEWKRVHSNIVTHIVGKSGLSDDNSNS 420
Query: 488 VLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEMMME 547
V +L LS + LP + LK CF Y ++FP+D+ + + L W+A+G + + ++
Sbjct: 421 VYRVLSLSYEDLP-MQLKHCFFYLAHFPEDYKIDVKILFNYWVAEGII--TPFHDGSTIQ 480
Query: 548 DIGEGYFNLLLSRSLFQDVFMDKY--GRIAYFKMHDLIHDVSCIISNHQKL--ELDLPNW 607
D GE Y L+ R++ V + Y RI Y +MHD++ +V + + + +P
Sbjct: 481 DTGESYLEELVRRNMV--VVEESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVVKVPTT 540
Query: 608 SE-----KGARNFRTLVNDNYKVLHT--HK--------------------LDFLC---LR 667
+ + R LV + LH HK F C LR
Sbjct: 541 TSTTINAQSPCRSRRLVLHSGNALHMLGHKDNKKARSVLIFGVEEKFWKPRGFQCLPLLR 600
Query: 668 VLVLN--PFDKIELLNSIGMLKHLRYLEIINY-VGRLPESITMLYNLQTLNLGTR----V 727
VL L+ F+ +L +SIG L HLR+L + V LP S+ L L LNLG V
Sbjct: 601 VLDLSYVQFEGGKLPSSIGDLIHLRFLSLYEAGVSHLPSSLGNLKLLLCLNLGVADRLLV 660
Query: 728 DLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFDEGCTIEELGPLKNL 787
+P L+++ LR+L+ R + + L++L++L F + G + +L
Sbjct: 661 HVPNVLKEMQELRYLRLPRSMPAKTKLELGDLVNLESLTNFST---------KHGSVTDL 720
Query: 788 KGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFI-WRNVNYEEEDNNHNDLEVLEGLQP 847
LS L + S L+ ++LR L+ + + + N+ +L VL+ +
Sbjct: 721 LRMTKLSVLNVIFSGECTFETLLLSLRELRNLETLSFHDFQKVSVANHGGELLVLDFIHL 780
Query: 848 HKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKC-TDCEKLPMLGQLPNLENLYISGMGS 907
S+ + F Q F +L I L C + + +P+L +L +L+++Y+S S
Sbjct: 781 KDLTLSMHLPRFPDQYR----FPPHLAHIWLIGCRMEEDPMPILEKLLHLKSVYLS---S 840
Query: 908 VRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQLWEKVTNGTAFPRLEGLGINHCSKLM 932
+G + FP L L + + L W +V G + P L L I++C KL
Sbjct: 841 GAFLGRRMVCSKGG----FPQLLALKMSYKKELVEW-RVEEG-SMPCLRTLTIDNCKKLK 873
BLAST of Spg005273 vs. TAIR 10
Match:
AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 207.2 bits (526), Expect = 6.6e-53
Identity = 233/921 (25.30%), Postives = 420/921 (45.60%), Query Frame = 0
Query: 68 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINRKRLHHQ 127
MAE + +F VQ+ L +L +E++ G+++++ L+ +L +++L+D + K+
Sbjct: 1 MAEGVVSFGVQK-LWALLNRESERLN---GIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 60
Query: 128 SVSMWVDNLQDIVYEADDLLDELVYEDLRQKVEIEKTKQKVRNFFSPSNNPFLF-RWKMA 187
V ++++++D+V++A+D+++ V LR E + K VR FL R K+A
Sbjct: 61 RVRNFLEDVKDLVFDAEDIIESYVLNKLRG--EGKGVKNHVRRLAC-----FLTDRHKVA 120
Query: 188 KKMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLKQFRET-TSILNFEVIGREVE 247
++ IT+ + + + SLG+ + + + ++ R+T + +++G E
Sbjct: 121 SDIEGITKRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQREIRQTFPNSSESDLVGVEQS 180
Query: 248 ASNILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFI 307
++ +++ N + ++ I GMGGIGKTTLA+ IF+H+LV+ HFD WVCVS+ F
Sbjct: 181 VEELVGPMVEIDN---IQVVSISGMGGIGKTTLARQIFHHDLVRRHFDGFAWVCVSQQFT 240
Query: 308 ITNILEGIFQSLTNTSGG-WKSKENLVRG-LKKEMQGKNYFLVLDDVWNEEATLWGELKD 367
++ + I Q L G + E ++G L + ++ Y +VLDDVW EE W +K+
Sbjct: 241 QKHVWQRILQELRPHDGEILQMDEYTIQGKLFQLLETGRYLVVLDDVWKEED--WDRIKE 300
Query: 368 CLKETAGKSGNVIIVTTRSGKVATIMETTS-SHHLNKLSDDQCWSLFKDSANANGLLMNQ 427
K G +++T+R+ V + T S L+ + W LF+ +
Sbjct: 301 VFPR---KRGWKMLLTSRNEGVGLHADPTCLSFRARILNPKESWKLFERIVPRRNETEYE 360
Query: 428 ELETVRVELVKKIDGVPLVAKVLGGAVTFEGNFERWVTEVKRVVKNISK--------DDK 487
E+E + E+V G+PL KVLGG + + W KRV +NI DD
Sbjct: 361 EMEAIGKEMVTYCGGLPLAVKVLGGLLANKHTASEW----KRVSENIGAQIVGKSCLDDN 420
Query: 488 DF--VLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENE 547
V IL LS + LP LK CF Y ++FP+D+ +L W A+G
Sbjct: 421 SLNSVYRILSLSYEDLP-TDLKHCFLYLAHFPEDYKIKTRTLYSYWAAEGIYDG------ 480
Query: 548 MMMEDIGEGYFNLLLSRSLFQDVFMDKYGRIAYFKMHDLIHDVSCI----ISNHQKLELD 607
+ + D GE Y L+ R+L + R+ +MHD++ +V CI + N ++ +
Sbjct: 481 LTILDSGEDYLEELVRRNLVIAEKSNLSWRLKLCQMHDMMREV-CISKAKVENFLQI-IK 540
Query: 608 LPN-----WSEKGARNFRTLVNDN--YKVL-HTHKLDFL--------------------- 667
+P ++ +R+ R V+ + +L H K+ L
Sbjct: 541 VPTSTSTIIAQSPSRSRRLTVHSGKAFHILGHKKKVRSLLVLGLKEDLWIQSASRFQSLP 600
Query: 668 CLRVLVLN--PFDKIELLNSIGMLKHLRYLEIIN-YVGRLPESITMLYNLQTLNL----G 727
LRVL L+ F+ +L +SIG L HLR+L + V LP +I L + LNL G
Sbjct: 601 LLRVLDLSSVKFEGGKLPSSIGGLIHLRFLSLHQAVVSHLPSTIRNLKLMLYLNLHVAIG 660
Query: 728 TRVDLPKNLRKLVRLRHLKFFRFRVQQMPSHMSQLIHLQTLDAFVVGFDEGCTIEELGPL 787
V +P L++++ LR+L + + L++L+ L F + + L
Sbjct: 661 VPVHVPNVLKEMLELRYLSLPLDMHDKTKLELGDLVNLEYLWCFSTQHSSVTDLLRMTKL 720
Query: 788 KNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDNNHNDLEVLEGL 847
+ S + + E +++ L + + L L FI+ Y + L+ +
Sbjct: 721 RFFGVSFS------ERCTFENLSSSLRQFRKLETLSFIYSRKTYMVDYVGEFVLDFI--- 780
Query: 848 QPHKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKC-TDCEKLPMLGQLPNLENLYISGM 907
+L+ LS+ ++ + ++ I L C + + +P+L +L +L+++ +
Sbjct: 781 ----HLKKLSLGVHLSKIPDQHQLPPHIAHIYLLFCHMEEDPMPILEKLLHLKSVELRRK 840
Query: 908 GSVRSIGSEFYGNNSNQRVYFPNLKTLSFNSMRNLQLWEKVTNGTAFPRLEGLGINHCSK 933
IG + FP L+ L + L+ W + + P L L I+ C K
Sbjct: 841 A---FIGRRMVCSKGG----FPQLRALQISEQSELEEW--IVEEGSMPCLRDLIIHSCEK 867
BLAST of Spg005273 vs. TAIR 10
Match:
AT3G07040.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 205.3 bits (521), Expect = 2.5e-52
Identity = 227/923 (24.59%), Postives = 401/923 (43.45%), Query Frame = 0
Query: 83 KVLKLAAEQIGVAWGLEKELSKLREELLHAEAILRDINR------KRLHHQSVSMWVDNL 142
++L + + + G+ E+ K+++ELL ++ L D ++ Q +V N
Sbjct: 12 RILSVLENETLLLSGVHGEIDKMKKELLIMKSFLEDTHKHGGNGSTTTTTQLFQTFVANT 71
Query: 143 QDIVYEADDLLDELVYEDLRQKVEIEKTKQKV-RNFFSPSNNPFLFRWKMAKKM------ 202
+D+ Y+ +D+LDE Y + ++ K+ R F P R +A+K+
Sbjct: 72 RDLAYQIEDILDEFGYH-----IHGYRSCAKIWRAFHFP--RYMWARHSIAQKLGMVNVM 131
Query: 203 -KDITETLDRHYLKAS-SLGVVGLVGEESTETNKNIDLKQFRETTSILNFEVIGREVEAS 262
+ I++++ R+Y + ++ + + + NI + ++G +
Sbjct: 132 IQSISDSMKRYYHSENYQAALLPPIDDGDAKWVNNIS----ESSLFFSENSLVGIDAPKG 191
Query: 263 NILKLVIDSSNKHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDNTLWVCVSEPFIIT 322
++ ++ + + ++ +VGMGG GKTTL+ IF + V+ HF++ WV +S+ ++I
Sbjct: 192 KLIGRLLSPEPQRI--VVAVVGMGGSGKTTLSANIFKSQSVRRHFESYAWVTISKSYVIE 251
Query: 323 NILEGIFQSLTNTSGGWKSKE-------NLVRGLKKEMQGKNYFLVLDDVWNEEATLWGE 382
++ + + + E LV L + +Q K Y +VLDDVW LW E
Sbjct: 252 DVFRTMIKEFYKEADTQIPAELYSLGYRELVEKLVEYLQSKRYIVVLDDVWT--TGLWRE 311
Query: 383 LKDCLKETAGKSGNVIIVTTRSGKVATIMETTSS--HHLNKLSDDQCWSLFKDSANANGL 442
+ L + G G+ +++TTR VA+ S H + L +D+ W LF + A L
Sbjct: 312 ISIALPD--GIYGSRVMMTTRDMNVASFPYGIGSTKHEIELLKEDEAWVLFSNKAFPASL 371
Query: 443 --LMNQELETVRVELVKKIDGVPLVAKVLGGAVT---FEGNFERWVTEVKRVVKNISKDD 502
Q LE + +LV++ G+PL LG ++ FE +++ + + + N +
Sbjct: 372 EQCRTQNLEPIARKLVERCQGLPLAIASLGSMMSTKKFESEWKKVYSTLNWELNN--NHE 431
Query: 503 KDFVLSILKLSVDRLPLLSLKQCFAYCSNFPKDHLFNKESLIRMWIAQGFVQSQKGENEM 562
V SI+ LS + LP LK+CF YCS FP ++ ++ LIRMW+AQ FV+ +G +
Sbjct: 432 LKIVRSIMFLSFNDLP-YPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIRG---V 491
Query: 563 MMEDIGEGYFNLLLSRSLFQDVFMDKYGRIAYFKMHDLIHDVSCIISNHQKLELDLPNWS 622
E++ + Y N L+ R++ Q + + +GR FKMHD+I +++ +S ++ D+ N
Sbjct: 492 KAEEVADSYLNELVYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERF-CDVYNDD 551
Query: 623 EKGARNFRTLVN--------------DNYKVLHTHKLDFLCLRVLVLNPFDKIELLNSIG 682
G T+ N D+ + + H L ++ + K+ELL S+
Sbjct: 552 SDGDDAAETMENYGSRHLCIQKEMTPDSIRATNLHSL------LVCSSAKHKMELLPSLN 611
Query: 683 MLKHLRYLEIINYVGRLPESITMLYNLQTLNLG-TRV-DLPKNLRKLVRLRHLKFFRFRV 742
+L+ L + + + +LP+ + ++NL+ LNL T+V +LPKN KLV L L ++
Sbjct: 612 LLRALDLED--SSISKLPDCLVTMFNLKYLNLSKTQVKELPKNFHKLVNLETLNTKHSKI 671
Query: 743 QQMPSHMSQLIHLQTLDAF--VVGFDEG-----------------------CTIEELGPL 802
+++P M +L L+ L F G D C E +
Sbjct: 672 EELPLGMWKLKKLRYLITFRRNEGHDSNWNYVLGTRVVPKIWQLKDLQVMDCFNAEDELI 731
Query: 803 KNLKGSLTLSNLERVKSKAEAINAKLMEKQDLRRLDFIWRNVNYEEEDNNHNDLEVLEGL 862
KNL L+ + V + E ++R+ F+ EEE +DL +
Sbjct: 732 KNLGCMTQLTRISLVMVRREHGRDLCDSLNKIKRIRFLSLTSIDEEEPLEIDDLIATASI 791
Query: 863 QP-------------HKNLQSLSIIGFGGQVLPSNIFVENLVAILLHKCTDCEKLPMLGQ 922
+ LQ+L+ +G G L N AIL + LP L
Sbjct: 792 EKLFLAGKLERVPSWFNTLQNLTYLGLRGSQLQEN-------AIL-----SIQTLPRLVW 851
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038877313.1 | 0.0e+00 | 66.32 | putative disease resistance protein RGA3 [Benincasa hispida] | [more] |
| XP_022145229.1 | 0.0e+00 | 65.34 | putative disease resistance protein RGA4 [Momordica charantia] | [more] |
| XP_022145177.1 | 0.0e+00 | 61.93 | putative disease resistance protein RGA3 [Momordica charantia] | [more] |
| XP_038895409.1 | 0.0e+00 | 61.34 | disease resistance protein RGA2-like isoform X1 [Benincasa hispida] | [more] |
| XP_022145104.1 | 0.0e+00 | 60.80 | disease resistance protein RGA2-like [Momordica charantia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q7XA40 | 5.0e-122 | 33.98 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA39 | 8.3e-117 | 32.85 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XBQ9 | 1.7e-114 | 33.53 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
| Q7XA42 | 1.5e-113 | 32.32 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q9LRR4 | 1.7e-109 | 30.25 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CUL8 | 0.0e+00 | 65.34 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A6J1CVR6 | 0.0e+00 | 61.93 | putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A6J1CU86 | 0.0e+00 | 60.80 | disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014... | [more] |
| A0A0A0KST5 | 2.8e-293 | 54.80 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1 | [more] |
| A0A0A0LBV0 | 7.7e-283 | 53.97 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G14470.1 | 1.2e-110 | 30.25 | NB-ARC domain-containing disease resistance protein | [more] |
| AT3G14460.1 | 1.3e-85 | 29.05 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT1G53350.1 | 8.9e-58 | 25.49 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
| AT5G35450.1 | 6.6e-53 | 25.30 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
| AT3G07040.1 | 2.5e-52 | 24.59 | NB-ARC domain-containing disease resistance protein | [more] |
