Spg005260 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg005260 vs. NCBI nr
Match: XP_022145225.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 1648.6 bits (4268), Expect = 0.0e+00
Identity = 839/1103 (76.07%), Postives = 953/1103 (86.40%), Query Frame = 0

Query: 27   VGQSTKRNMAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDA 86
            +G+S  RNMAEF+WTFAV+E+LKKTV+LAAEQ+G+AWGF KELSKL +SL+MVEAIL D 
Sbjct: 33   LGESQNRNMAEFLWTFAVQEVLKKTVRLAAEQIGMAWGFNKELSKLRDSLVMVEAILRDV 92

Query: 87   DRIKA--EHQALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGK-DLMVSN-FVSFSK 146
            DRIKA  EH ALKLW+EKL+DIVFEADVLLD++AYED+RRKVETGK  +MVSN F S SK
Sbjct: 93   DRIKAEPEHHALKLWVEKLQDIVFEADVLLDDIAYEDLRRKVETGKVQVMVSNCFASSSK 152

Query: 147  NPLVFRFKMANKIKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVI 206
            NP++FR KMA KIK IAK LD HY  AS VGLVA TSKE E DLSQI ETDSFLDEIGVI
Sbjct: 153  NPIIFRLKMARKIKVIAKMLDRHYYRASAVGLVARTSKETEPDLSQIQETDSFLDEIGVI 212

Query: 207  GRETEVSEIVNKLIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCV 266
            GRE+EVSEIVNKLI+LS+QEAL++LPIVG+ GLGKT+LAK IF+H +I+ +FD+ IWVCV
Sbjct: 213  GRESEVSEIVNKLIELSNQEALSILPIVGMPGLGKTSLAKVIFNHGLIKANFDEIIWVCV 272

Query: 267  SEPFVIKNILRAILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSE 326
            SEPFVI  ILR++LET N N GGL+ KE ILQELQKLL+ KKYFLV DDVWNENP+LWSE
Sbjct: 273  SEPFVINKILRSVLETLNPNSGGLESKEAILQELQKLLNAKKYFLVFDDVWNENPDLWSE 332

Query: 327  LRACLLKINKKFGSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPV 386
            LRACLLKI ++ G+V+VVTTRS+EVAKIMETHHRHHLRKL N+ CWSLFEKCAFG+ELP+
Sbjct: 333  LRACLLKITQESGNVLVVTTRSEEVAKIMETHHRHHLRKLSNNQCWSLFEKCAFGNELPI 392

Query: 387  IPRVDVVREMLVKKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSV 446
            IP++DVV+E L+KKFGGIPLVVKVLGG+VKL+K N EGLQS+LEN++RIPLQD++H+LS 
Sbjct: 393  IPKMDVVKEELIKKFGGIPLVVKVLGGIVKLEK-NPEGLQSTLENIMRIPLQDKSHVLST 452

Query: 447  IKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEK 506
            IKLSVDRLPS SLKQCFAYCS FP  FLF+KE+L+QMWIA+GFIQL +GSN++MEDIGEK
Sbjct: 453  IKLSVDRLPSPSLKQCFAYCSKFPPDFLFVKETLIQMWIARGFIQLSNGSNLMMEDIGEK 512

Query: 507  YFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDE-V 566
            YFNILLSRSLFQDVV+DNRGRII+CKMHDLIHD+ACAISNAQ+LQLDL +D K   D+ V
Sbjct: 513  YFNILLSRSLFQDVVKDNRGRIIHCKMHDLIHDVACAISNAQSLQLDLLIDGKRQRDKAV 572

Query: 567  LSIGHKIRTVHCSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKL 626
            LSIGH+IRT++CSEN  GKFH     SHVFHN+IT FIYLRVLI H+WFIRKL +SI KL
Sbjct: 573  LSIGHRIRTLYCSENGVGKFH-----SHVFHNRITSFIYLRVLIVHSWFIRKLSDSIVKL 632

Query: 627  KHLRYLDISHSRIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQ 686
            KHLRYLDISHS IRELPKSIVLLYNLQTL+LGSDT DLP  LRKLVNLRHLEF  N S+Q
Sbjct: 633  KHLRYLDISHSLIRELPKSIVLLYNLQTLKLGSDTVDLPKNLRKLVNLRHLEFPINPSNQ 692

Query: 687  TKQMPRHLSRLIQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAA 746
            TKQMPRHLSRLIQLQTLSSFVVG D+GCKIEELGPLK LRGELSL CLERVKS+EEAMAA
Sbjct: 693  TKQMPRHLSRLIQLQTLSSFVVGFDEGCKIEELGPLKNLRGELSLFCLERVKSREEAMAA 752

Query: 747  NLAEKENLSQLYFQWSLR-CEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAF 806
            NLAEKEN+S LY QW LR CEREDC+YND+NVLEGLQPH+NLQVLKIQNFAGELLP+  F
Sbjct: 753  NLAEKENISDLYLQWGLRSCEREDCHYNDMNVLEGLQPHRNLQVLKIQNFAGELLPNCIF 812

Query: 807  VENLVEIYLYDCKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLK 866
            VE LVEIYL+DCKRCETLPMLGQL+KLEVL IR LHSVKSIG EFYGNY  E TLFPKLK
Sbjct: 813  VEKLVEIYLHDCKRCETLPMLGQLSKLEVLRIRCLHSVKSIGHEFYGNYRHEMTLFPKLK 872

Query: 867  TFHISQMNSLEHWEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGP 926
            TFHI QMN+LEHWEEIETV NGT FP+LESL I+ CLKL N+PNFFAS S       +GP
Sbjct: 873  TFHIFQMNNLEHWEEIETVPNGTIFPYLESLKIVCCLKLRNVPNFFASDSE------EGP 932

Query: 927  PKLRSLKIVGCEKLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITN-FQ 986
             KL SL I  CE LT+LPN +E C SLE++ I KCPN+NSPPNLQ+M  LSSL I N   
Sbjct: 933  AKLLSLTIHECENLTKLPNGIEFCSSLENICISKCPNLNSPPNLQNMPKLSSLTIRNCIP 992

Query: 987  RLPEGLARLHNLKRLQVQGELRGFDWSPFTHLNSLESLVLLDMGSCLSQLPQQLEHLIAL 1046
            +L EGL  + NLK L++QG+ + +DWSP   L+SLE+L LL+MGSC +Q+PQQL+HL  L
Sbjct: 993  KLLEGLPCICNLKTLRIQGKWQEYDWSPMIQLSSLENLELLEMGSC-TQIPQQLQHLTTL 1052

Query: 1047 RSLYIVCFNGVESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQ 1106
            RSLYI   + +E+LPEWLGN+ SLE LNL+ CRNLKNLPSKEAMS LTRLNRL VHGCSQ
Sbjct: 1053 RSLYIGRCDSIETLPEWLGNLTSLEKLNLFGCRNLKNLPSKEAMSNLTRLNRLEVHGCSQ 1112

Query: 1107 IELDEGGFEREKVSHVPHIDFVP 1123
            IEL EGGFER KVSHVPHIDFVP
Sbjct: 1113 IELGEGGFERAKVSHVPHIDFVP 1122

BLAST of Spg005260 vs. NCBI nr
Match: XP_004152291.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KGN53095.1 hypothetical protein Csa_015377 [Cucumis sativus])

HSP 1 Score: 1549.6 bits (4011), Expect = 0.0e+00
Identity = 811/1099 (73.79%), Postives = 924/1099 (84.08%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MA+F+W+FAV+E+LKKTVKL AEQ+G++WGFKK+LSKL +SLLMVEAIL D +RIKAEHQ
Sbjct: 1    MADFLWSFAVDEVLKKTVKLVAEQIGMSWGFKKDLSKLRDSLLMVEAILRDVNRIKAEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            AL+LW+EKLE IVFEADVLLDEL+YED+RRKV+      V +FVS SKNPLVFR KMANK
Sbjct: 61   ALRLWVEKLEHIVFEADVLLDELSYEDLRRKVDARP---VRSFVSSSKNPLVFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            IKAIAK+LDEHY AASI+GLVA+TSKEVE++ SQILETDSFLDEIGVIGRE EV EIVNK
Sbjct: 121  IKAIAKRLDEHYCAASIMGLVAITSKEVESEPSQILETDSFLDEIGVIGREAEVLEIVNK 180

Query: 215  LIDLSSQE-ALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILR 274
            L++LS QE AL+VLPIVG+GGLGKT+LAK IFHHEMIRE+FD+ IWVCVSEPFVI  ILR
Sbjct: 181  LLELSKQEAALSVLPIVGIGGLGKTSLAKAIFHHEMIRENFDRMIWVCVSEPFVINKILR 240

Query: 275  AILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKK 334
            AILET NANFGGLD KE +LQELQKLL +KKYFLVLDDVWNENP+LW+ELRACLLK NKK
Sbjct: 241  AILETLNANFGGLDNKEALLQELQKLLRNKKYFLVLDDVWNENPDLWNELRACLLKANKK 300

Query: 335  FGSVVVVTTRSDEVAKIMETHH-RHHLRKLPNDHCWSLFEKCAFGSELPVIPRVD-VVRE 394
            FGSV+VVTTRSDEVA I+ET+H RH LRKL ND+CW+LFEKCAFGS+LPV PRVD V+RE
Sbjct: 301  FGSVIVVTTRSDEVANIVETNHQRHRLRKLSNDYCWTLFEKCAFGSDLPVTPRVDHVIRE 360

Query: 395  MLVKKFGGIPLVVKVLGGMVKLDKSN-YEGLQSSLENLIRIPLQDENHILSVIKLSVDRL 454
             LVK+FGGIPLVVKV GGMVKLDK+   +GL+S+LENLI  PLQ EN ILS IKLSVDRL
Sbjct: 361  ELVKRFGGIPLVVKVFGGMVKLDKNKCCQGLRSTLENLIISPLQYENSILSTIKLSVDRL 420

Query: 455  PSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSR 514
            PSSSLKQCFAYCSNFPRGFLFI+E LVQMWIAQGFI LPSGSNV MEDIG  YFN LLSR
Sbjct: 421  PSSSLKQCFAYCSNFPRGFLFIREPLVQMWIAQGFIHLPSGSNVTMEDIGANYFNTLLSR 480

Query: 515  SLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRT 574
            SLFQDVV+D+R RI+YCKMHD++HD+ACAISNAQ L+L      KS  D+ LSIGH+IRT
Sbjct: 481  SLFQDVVKDDRERILYCKMHDVVHDVACAISNAQKLRL----SGKSNGDKALSIGHEIRT 540

Query: 575  VHCSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDIS 634
            +HCSENV  +FH+ T DSHVFHN+I++F YL VLI H+WFI +LP+SIAKLKHLRYLDIS
Sbjct: 541  LHCSENVVERFHLPTFDSHVFHNEISNFTYLCVLIIHSWFIHQLPDSIAKLKHLRYLDIS 600

Query: 635  HSRIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLS 694
            HS IR LP SIV LYNLQTLRLGS    LPTKLRKLVNLRHLEF  +LS QTKQMP+HLS
Sbjct: 601  HSLIRTLPDSIVSLYNLQTLRLGSKIMHLPTKLRKLVNLRHLEF--SLSTQTKQMPQHLS 660

Query: 695  RLIQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLS 754
            RL+QLQTLSSFVVG DKGCKIEELGPL  L+GELSL  LE VKSK EAMAANLA KEN+S
Sbjct: 661  RLLQLQTLSSFVVGFDKGCKIEELGPLNNLKGELSLFHLEHVKSKTEAMAANLAMKENIS 720

Query: 755  QLYFQWSLRCEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLY 814
             LYFQWSL  EREDC+ NDLNVLEGL+PHKNLQ LKI+NF G +LP+G FVENLVE+ LY
Sbjct: 721  DLYFQWSLLSEREDCSNNDLNVLEGLRPHKNLQALKIENFGG-VLPNGLFVENLVEVILY 780

Query: 815  DCKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGN---YHDE--RTLFPKLKTFHIS 874
            DCKRCETLPMLG L+KLE+LHIR L SVKSIGDEFYGN   YH+E    LFPKLKT HIS
Sbjct: 781  DCKRCETLPMLGHLSKLELLHIRCLDSVKSIGDEFYGNNNSYHNEWSSLLFPKLKTLHIS 840

Query: 875  QMNSLEHWEEIETVSN-GTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLR 934
            QM SLE W+EI + SN G TFPHLESLSI++C KLMNIPN            FQ PPKL+
Sbjct: 841  QMKSLELWQEIGSSSNYGATFPHLESLSIVWCSKLMNIPNL-----------FQVPPKLQ 900

Query: 935  SLKIVGCEKLTELPNWLELCHSLEDMSICKCPNV--NSPPNLQDMQNLSSLIITNFQRLP 994
            SLKI  CEKLT+LP+WL LC S+E+M IC CPNV  NS PNL+ M NLSSL I  F++LP
Sbjct: 901  SLKIFYCEKLTKLPHWLNLCSSIENMVICNCPNVNNNSLPNLKSMPNLSSLSIQAFEKLP 960

Query: 995  EGLARLHNLKRLQVQGELRGFDWSPFTHLN-SLESLVLLDMG--SCLSQLPQQLEHLIAL 1054
            EGLA +HNLKRL V GEL+G DWSPF +LN S+E L L++ G  + L QLP+QLE+L AL
Sbjct: 961  EGLATIHNLKRLDVYGELQGLDWSPFMYLNSSIEILRLVNTGVSNLLLQLPRQLEYLTAL 1020

Query: 1055 RSLYIVCFNGVESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQ 1114
            RSL I  F+ ++SLPEWLGN+ SLETLNL  C+NLK+ PS EAMS LT+L+RL  + C Q
Sbjct: 1021 RSLDIERFSDIDSLPEWLGNLTSLETLNLRYCKNLKSFPSIEAMSNLTKLSRLETYECFQ 1078

Query: 1115 IELDEGGFEREKVSHVPHI 1119
            ++LDEG +ER K++HV  I
Sbjct: 1081 LKLDEGSYERAKIAHVHDI 1078

BLAST of Spg005260 vs. NCBI nr
Match: XP_038900029.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 1540.4 bits (3987), Expect = 0.0e+00
Identity = 779/1089 (71.53%), Postives = 909/1089 (83.47%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MAEF+WTFA +ELLKKTVKLAA+Q+GLAWGFK ELS L +SLLMVEAIL D DRIKAEHQ
Sbjct: 1    MAEFLWTFAAQELLKKTVKLAAKQIGLAWGFKNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            A+KLW+EKLEDIVFEADVLLDELAYED+RRK E  K++ VSNFVSFSKNPLVFR KMANK
Sbjct: 61   AVKLWVEKLEDIVFEADVLLDELAYEDIRRKAEPQKEVTVSNFVSFSKNPLVFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            IK IA+ L++HYSAAS VGLVA+ SK++E D SQI ETDSF DE GVIGRE EV EIV  
Sbjct: 121  IKNIARMLEKHYSAASTVGLVAIISKQIEPDFSQIQETDSFPDEFGVIGREAEVLEIVKI 180

Query: 215  LIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRA 274
             +DLS +EA++VLPIVG+GGLGKTALAK IF+HE+I+ +FD+TIWVCVSEPF+IK ILR 
Sbjct: 181  SVDLSYREAVSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTIWVCVSEPFLIKKILRT 240

Query: 275  ILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKF 334
            ILET N NFGGLD KE +LQELQKLL+DKKYFLVLDDVWNENP LW+EL++CLLKI+++ 
Sbjct: 241  ILETLNPNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKSCLLKISQRS 300

Query: 335  GSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLV 394
            G+VVVVTTRS  VA+IMETH R+HL KL +DHCWSLF+K AFG+E   IP +D+V++ LV
Sbjct: 301  GNVVVVTTRSGRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNEFLGIPELDIVQKELV 360

Query: 395  KKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSS 454
            K+FGGIPLVVKVLGG+VK D++ +EGLQ +LENL++I LQDENH++S IKL+VDRLP SS
Sbjct: 361  KRFGGIPLVVKVLGGIVKFDET-HEGLQQTLENLMKIQLQDENHVVSTIKLTVDRLPLSS 420

Query: 455  LKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ 514
            LKQCFAYCSNFP+ F F KE+L+QMWIAQGFIQ P GS+ +MEDIGEKYFN+LLSR LFQ
Sbjct: 421  LKQCFAYCSNFPKDFKFRKEALIQMWIAQGFIQPPEGSDEMMEDIGEKYFNVLLSRLLFQ 480

Query: 515  DVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDL--SMDEKSWSDEVLSIGHKIRTVH 574
            D+V+DNRGRI++CKMHDLIHD+A AISN+Q L LD    +D + W  E  S  HKIRT  
Sbjct: 481  DIVKDNRGRIVFCKMHDLIHDVALAISNSQELILDPLDLLDGEPWRREACSASHKIRTPD 540

Query: 575  CSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHS 634
            C+EN + K HM T DSHVFHNKITDFIYLRVLIAH+WFI KLPNSIAKLKHLRYLDIS+S
Sbjct: 541  CNENHSRKLHMLTFDSHVFHNKITDFIYLRVLIAHSWFICKLPNSIAKLKHLRYLDISYS 600

Query: 635  RIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRL 694
             IRELP+S+ LLYNLQTL+L     DLP  LRKLV+LRHLEFFA+    TKQMP+HL +L
Sbjct: 601  TIRELPESLFLLYNLQTLKLSRFLNDLPKNLRKLVSLRHLEFFAD-PCDTKQMPQHLGKL 660

Query: 695  IQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQL 754
            IQLQTLSSFVVG D GCKIEELGPL+ L+G+L+L CLERVKSK+EAMAANL EK N+S L
Sbjct: 661  IQLQTLSSFVVGFDDGCKIEELGPLRNLKGKLNLLCLERVKSKKEAMAANLVEKGNISFL 720

Query: 755  YFQWSLRCER-EDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYD 814
            YF W+LRCER E  NYNDLNVLEGLQPHKNLQ L+I NF G+LLP+G FVENLVEIYL+D
Sbjct: 721  YFHWTLRCERSEGSNYNDLNVLEGLQPHKNLQALRIHNFLGKLLPNGIFVENLVEIYLHD 780

Query: 815  CKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLE 874
            C+ CE LPMLGQL+KLEVL +R LHSV SIG+EFYGNY ++RTLFPKLK FHI++M SLE
Sbjct: 781  CEICEALPMLGQLSKLEVLELRCLHSVGSIGEEFYGNYREKRTLFPKLKAFHINEMISLE 840

Query: 875  HWEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGC 934
            +WEEI  VSNGT FPHLESLSI+ C +L NIPNFFASH    FPSFQG  KLRSLKI+GC
Sbjct: 841  NWEEIMVVSNGTIFPHLESLSIVCCPRLTNIPNFFASHHESSFPSFQGSTKLRSLKILGC 900

Query: 935  EKLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNL 994
            E L +LPN LE C SLE+M I     +NSPP LQ+MQNL+SL +T F++LP+GLA+L  L
Sbjct: 901  ENLRKLPNDLEFCSSLENMRISNSSKLNSPPRLQNMQNLTSLSMTEFRKLPDGLAQLRKL 960

Query: 995  KRLQVQGELRGFDWSPFTHLNSLESLVLLDM-GSCLSQLPQQLEHLIALRSLYIVCFNGV 1054
            K+L V G L+G+DWSP  HL SLE+L+L+D+ GS   QLPQQL  L +LRSL+I  FNGV
Sbjct: 961  KKLSVHGYLQGYDWSPLVHLGSLENLMLVDLEGSGAVQLPQQLAQLTSLRSLHISHFNGV 1020

Query: 1055 ESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFERE 1114
            E LPE  GN  SLE L LY+C  LKN+PSKEAMSKLTRL  L V+GCSQ+EL+EG FE  
Sbjct: 1021 EDLPECFGNFTSLEVLKLYNCVKLKNMPSKEAMSKLTRLKSLRVYGCSQLELNEGDFEWV 1080

Query: 1115 KVSHVPHID 1120
             +SHVP I+
Sbjct: 1081 NISHVPTIN 1087

BLAST of Spg005260 vs. NCBI nr
Match: XP_022145224.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 1520.4 bits (3935), Expect = 0.0e+00
Identity = 770/1083 (71.10%), Postives = 896/1083 (82.73%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MAEF+WTFA +ELLK+ VKLAAEQ  LAWGF KEL KL +SLLMVEAIL D DRIK EHQ
Sbjct: 1    MAEFLWTFAAQELLKQAVKLAAEQTALAWGFNKELLKLRDSLLMVEAILRDVDRIKGEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            A+KLW+EKL++IVFEADVLLD+LAYE +RRKVE G + MVSNFVS SKNPL FR KMANK
Sbjct: 61   AVKLWVEKLQEIVFEADVLLDDLAYEHLRRKVEAGNEAMVSNFVSISKNPLAFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            I+AIA+ LD+HYS+A+ VGLVA+T KE+E D SQI ETDSFLDE GVIGRE EVSEIVN+
Sbjct: 121  IRAIARMLDKHYSSAAAVGLVAVTPKEIEPDFSQIQETDSFLDEFGVIGREIEVSEIVNR 180

Query: 215  LIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRA 274
            LID S ++AL+VLPIVG+GGLGKTAL K +F+HE I+ +FD+TIWVCVSEPFVIK ILRA
Sbjct: 181  LIDFSHRDALSVLPIVGMGGLGKTALMKVVFNHEFIKGNFDRTIWVCVSEPFVIKKILRA 240

Query: 275  ILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKF 334
            ILET N NFGGLD KE +LQELQKLL+DK+ FLVLDDVWNEN  LW+EL+ACLLKI+++ 
Sbjct: 241  ILETLNPNFGGLDSKEALLQELQKLLNDKRCFLVLDDVWNENLTLWNELKACLLKISQRS 300

Query: 335  GSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLV 394
            G+ VVVTTRSD+VA++METH RHHL+ L +DHCWSLF+   FG+E   I  +D+V+  LV
Sbjct: 301  GNTVVVTTRSDKVAEVMETHPRHHLKSLSDDHCWSLFKSYVFGNESLDIQELDIVKGELV 360

Query: 395  KKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSS 454
            K+FGGIPLVVKVLGG VK D+ N EGLQS+LENL+RIPL+ ENH+LS IKLSVDRLPSSS
Sbjct: 361  KRFGGIPLVVKVLGGTVKFDE-NSEGLQSTLENLMRIPLEGENHVLSTIKLSVDRLPSSS 420

Query: 455  LKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ 514
            LKQCFAYCSNFP+ F F K++L+QMWIAQGFIQ   GSNV MED+G+KYFN+LLSRSLFQ
Sbjct: 421  LKQCFAYCSNFPQDFEFRKQALIQMWIAQGFIQSLGGSNVTMEDVGDKYFNVLLSRSLFQ 480

Query: 515  DVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDL--SMDEKSWSDEVLSIGHKIRTVH 574
            DVV+D+RGRIIYCKMHDLIHD+AC ISNAQ L+L+L  S+D      E  SIGH+IRT+ 
Sbjct: 481  DVVKDSRGRIIYCKMHDLIHDVACVISNAQKLKLNLLDSLDGIRRRGEDRSIGHRIRTLD 540

Query: 575  CSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHS 634
            CSEN A K H+   DSHVFHNKI DF+YLRVLIAH+WFI KLP+S+AKLKHLRY+DISHS
Sbjct: 541  CSENDARKLHVLAFDSHVFHNKILDFVYLRVLIAHSWFINKLPDSVAKLKHLRYIDISHS 600

Query: 635  RIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRL 694
             IRELP+SIVLLYNLQTLRLGS   DLP  LRKLV+LRHLE F N  + T QMP+ L +L
Sbjct: 601  TIRELPESIVLLYNLQTLRLGSFLKDLPKNLRKLVSLRHLEIFTNPCYST-QMPQRLGQL 660

Query: 695  IQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQL 754
             QLQTLSSFVVG D+GC+IEELG LK L+G+LSL  LERVKSK+EAM A L EK+N+S L
Sbjct: 661  SQLQTLSSFVVGFDEGCRIEELGTLKNLKGKLSLLRLERVKSKKEAMTAKLVEKDNISDL 720

Query: 755  YFQWSLRCEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYDC 814
            YFQW L  ER+  NYNDLNVLEGLQPHKNLQVL+I NFAGELLP+G FVE LVEIYL+ C
Sbjct: 721  YFQWCLIGERQGSNYNDLNVLEGLQPHKNLQVLRIHNFAGELLPNGTFVEKLVEIYLHGC 780

Query: 815  KRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLEH 874
            +RC+TLPMLGQL+KLEVL IR LHSV+SIG+EFYGNY D+RTLF KL+TFHIS+M+SLEH
Sbjct: 781  ERCKTLPMLGQLSKLEVLEIRDLHSVRSIGEEFYGNYRDKRTLFHKLRTFHISEMSSLEH 840

Query: 875  WEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCE 934
            WEEI  V NGT FPHLESLSI+ CL+L +IPNFFASH         G  KLRSLKI+GCE
Sbjct: 841  WEEIRAVPNGTIFPHLESLSIVCCLRLTSIPNFFASHEEERL----GSAKLRSLKILGCE 900

Query: 935  KLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNLK 994
             LTELPN LE C SL+ M I  C N+NS P+LQ+M NLSSL IT FQ+LPEGLA++ NLK
Sbjct: 901  NLTELPNGLEFCSSLKTMWIGNCSNLNSVPSLQNMHNLSSLSITEFQKLPEGLAQVRNLK 960

Query: 995  RLQVQGELRGFDWSPFTHLNSLESLVLLDMGSCLS-QLPQQLEHLIALRSLYIVCFNGVE 1054
             L V G L+G+DWSP  HL SLE+LVL D G   + QLPQQLEHL  LRSL+I  F+GVE
Sbjct: 961  SLSVHGYLQGYDWSPLVHLGSLENLVLADFGGTAAIQLPQQLEHLTNLRSLHISHFDGVE 1020

Query: 1055 SLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFEREK 1114
            +LPEW  N  SLE L +Y+CR LKN+PSKEAMSKLTRL  L V+GCS++E+ +  FER K
Sbjct: 1021 ALPEWFRNFTSLEILKVYNCRKLKNMPSKEAMSKLTRLKSLRVYGCSKLEVGQCDFERVK 1077

BLAST of Spg005260 vs. NCBI nr
Match: KAA0044611.1 (putative disease resistance protein RGA3 [Cucumis melo var. makuwa])

HSP 1 Score: 1490.7 bits (3858), Expect = 0.0e+00
Identity = 754/1088 (69.30%), Postives = 900/1088 (82.72%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MAEF+WTFA +ELLKKTVKLAAEQ+GLAWGF  ELS L +SLLMVEAIL D DRIKAEHQ
Sbjct: 1    MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            A+KLW+EKLE IVFE DVLLDELAYED+RRKVE+ K+ +VSNF+SFSK PLVFR KMANK
Sbjct: 61   AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            IK IAK L++HYSAAS VGLVA+ SK+ E D SQI ETDSFLDE GVIGRETEV EIVN 
Sbjct: 121  IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 215  LIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRA 274
             +DLS +E L+VLPIVG+GGLGKTALAK IF+HE+I+ +FD+T+WVCVSEPF+IK ILRA
Sbjct: 181  SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 275  ILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKF 334
            ILET N+NFGGLD KE +LQELQKLL+DKKYFLVLDDVWNENP LW+EL+ CLLKI+++ 
Sbjct: 241  ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 335  GSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLV 394
            G+VVVVTTRSD VA+IMETH R+HL KL +DHCWSLF+K AFG+EL  IP +D+V++ LV
Sbjct: 301  GNVVVVTTRSDRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKELV 360

Query: 395  KKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSS 454
            K+FGGIPL VKV+GG+VK D+ N+EGLQ SLENL+R+ LQDENH++S IKL+VDRLP SS
Sbjct: 361  KRFGGIPLAVKVMGGIVKFDE-NHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLSS 420

Query: 455  LKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ 514
            LKQCFAYCSNFP+ F F +E+L+QMWIAQGFIQ    S+ +MEDIGEKYFN+LLSR LFQ
Sbjct: 421  LKQCFAYCSNFPKDFKFRREALIQMWIAQGFIQPSLESDEMMEDIGEKYFNVLLSRFLFQ 480

Query: 515  DVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLS--MDEKSWSDEVLSIGHKIRTVH 574
            D+V+DNRGRII+CKMHDLIHD+ACAISN+Q L+ D S  +D + W  +  S  H+++T  
Sbjct: 481  DIVKDNRGRIIFCKMHDLIHDVACAISNSQGLKWDPSDLLDGEPWRRQACSASHELKTPD 540

Query: 575  CSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHS 634
            C+EN + K HM T DSHVFHNK+TDFI+LRVLIAH+WFI KLPNSIAKLKHLRYLDIS+S
Sbjct: 541  CNENHSRKLHMLTFDSHVFHNKVTDFIHLRVLIAHSWFICKLPNSIAKLKHLRYLDISYS 600

Query: 635  RIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRL 694
             IRELP S+VLLYNLQTL+L     DLP  LRKLV+LRHLEFF++  + TKQMP+HL +L
Sbjct: 601  TIRELPDSVVLLYNLQTLKLSRFFNDLPKNLRKLVSLRHLEFFSDPCN-TKQMPQHLGKL 660

Query: 695  IQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQL 754
            IQLQTLSSFVVG D GCKIEEL  L+ L+G L+L CLERVKSK EAMAANL EK N+S L
Sbjct: 661  IQLQTLSSFVVGFDDGCKIEELRSLRNLKGNLNLLCLERVKSKMEAMAANLVEKGNISFL 720

Query: 755  YFQWSLRCER-EDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYD 814
            YF W+LR ER E  NYNDLNVLEGLQPHKNLQ L+I+NF G+LLP+  FVENLVEIYL++
Sbjct: 721  YFYWTLRSERSEGSNYNDLNVLEGLQPHKNLQALRIRNFLGKLLPNVIFVENLVEIYLHE 780

Query: 815  CKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLE 874
            C+ CETLP LGQL+KLEVL +R L SV+SIG+EFYGNY ++R LFP LKTFHI +M +LE
Sbjct: 781  CEICETLPTLGQLSKLEVLELRCLCSVRSIGEEFYGNYREKRILFPTLKTFHICEMINLE 840

Query: 875  HWEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGC 934
            +WEEI  VSNGT F +LESL I+ C +L +IPN FA H    FPSFQ   KLRSLKI+GC
Sbjct: 841  NWEEIMVVSNGTIFSNLESLYIVCCPRLTSIPNLFAYHHESSFPSFQLSAKLRSLKILGC 900

Query: 935  EKLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNL 994
            E L + PN LE C SLE+M I  C N+N PP+L++MQNL+SL IT F++LP+GLA++  L
Sbjct: 901  ESLQKQPNGLEFCSSLENMWISNCSNLNYPPSLRNMQNLTSLSITEFRKLPDGLAQVCKL 960

Query: 995  KRLQVQGELRGFDWSPFTHLNSLESLVLLDM-GSCLSQLPQQLEHLIALRSLYIVCFNGV 1054
            + L V G L+G+DWSP  HL+SLE+LVL+D+ GS   QLP QLE L +LRSL++  F+G+
Sbjct: 961  RSLSVHGYLQGYDWSPLVHLDSLENLVLVDLEGSGAIQLPHQLEQLTSLRSLHLSHFSGI 1020

Query: 1055 ESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFERE 1114
            E+LPEW GN  SLE L LY+C NLKN+PSKEA+SKLTRL  L V+GC Q++LD G FER 
Sbjct: 1021 EALPEWFGNFTSLEILKLYNCVNLKNMPSKEAISKLTRLTSLRVYGCPQLKLDGGDFERV 1080

Query: 1115 KVSHVPHI 1119
             +S VP +
Sbjct: 1081 NISLVPTV 1086

BLAST of Spg005260 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 444.1 bits (1141), Expect = 4.6e-123
Identity = 339/1005 (33.73%), Postives = 524/1005 (52.14%), Query Frame = 0

Query: 58   QMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQALKLWLEKLEDIVFEADVLLDEL 117
            ++GL +GF+KE  KL++   M++A+L DA   + +++A+K WL+KL    +E D +LD+ 
Sbjct: 20   ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 118  AYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANKIKAIAKKLDEHYSAASIVGLVAM 177
              E  R K              +    + F +K+  ++K + +KLD   + A       +
Sbjct: 80   KTEAARFKQAV--------LGRYHPRTITFCYKVGKRMKEMMEKLD---AIAEERRNFHL 139

Query: 178  TSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNKLI-DLSSQEALAVLPIVGVGGLG 237
              + +E   ++  +T   L E  V GRE E  EIV  LI ++S  E + VLPI+G+GGLG
Sbjct: 140  DERIIERQAAR-RQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLG 199

Query: 238  KTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNA-NFGGLDRKEIILQE 297
            KT LA+ +F+ + I E F+  IWVCVS+ F  K +++AI+E+    + G +D    + ++
Sbjct: 200  KTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAP-LQKK 259

Query: 298  LQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKFGSVVVVTTRSDEVAKIMETHH 357
            LQ+LL+ K+YFLVLDDVWNE+   W  LRA +LKI    G+ +++TTR +++  IM T  
Sbjct: 260  LQELLNGKRYFLVLDDVWNEDQEKWDNLRA-VLKIGAS-GASILITTRLEKIGSIMGTLQ 319

Query: 358  RHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLVKKFGGIPLVVKVLGGMV--KL 417
             + L  L  + CW LF++ AF  +    P++  + + +VKK GG+PL  K LGG++  K 
Sbjct: 320  LYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKR 379

Query: 418  DKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIK 477
            ++S +E ++ S   +  +P QDEN +L  ++LS   LP   L+QCFAYC+ FP+     K
Sbjct: 380  EESEWEHVRDS--EIWNLP-QDENSVLPALRLSYHHLP-LDLRQCFAYCAVFPKDTKIEK 439

Query: 478  ESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQDVVRDNRGRIIYCKMHDLI 537
            E L+ +W+A  F  L S  N+ +ED+G + +N L  RS FQ++  + +    Y KMHDLI
Sbjct: 440  EYLIALWMAHSF--LLSKGNMELEDVGNEVWNELYLRSFFQEI--EVKSGKTYFKMHDLI 499

Query: 538  HDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRTVHCSENVAGKFHMSTSDSHVFHN 597
            HD+A ++ +A      +        ++++ I   +       ++     +S+    +F  
Sbjct: 500  HDLATSMFSASASSRSIRQINVKDDEDMMFI---VTNYKDMMSIGFSEVVSSYSPSLFKR 559

Query: 598  KITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHSRIRELPKSIVLLYNLQTLRLG 657
                F+ LRVL        +LP+S+  L HLRYLD+S ++I  LPK +  L NLQTL L 
Sbjct: 560  ----FVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLY 619

Query: 658  S--DTTDLPTKLRKLVNLRHLEFFANLSH-QTKQMPRHLSRLIQLQTLSSFVVGCDKGCK 717
            +    + LP +  KL +LR+L     L H     MP  +  L  L+TL  FVVG  KG +
Sbjct: 620  NCQSLSCLPKQTSKLCSLRNLV----LDHCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQ 679

Query: 718  IEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSLRCEREDCNYNDL 777
            + EL  L  LRG +S++ LERVK+  EA  ANL+ K NL  L   W      E     ++
Sbjct: 680  LGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDRPNRYES---EEV 739

Query: 778  NVLEGLQPHKNLQVLKIQNFAGELLP---SGAFVENLVEIYLYDCKRCETLPMLGQLAKL 837
             VLE L+PH NL+ L+I +F G  LP   + + ++N+V I +  C+ C  LP  G+L  L
Sbjct: 740  KVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCL 799

Query: 838  EVLHIR-SLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLEHWEEIETVSNGTTFP 897
            E L ++     V+ + D  +      R  FP L+  HI    +L   + ++ +     FP
Sbjct: 800  ESLELQDGSVEVEYVEDSGFLT----RRRFPSLRKLHIGGFCNL---KGLQRMKGAEQFP 859

Query: 898  HLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEKLTELPNWLELCHS 957
             LE + I  C      P F       +FP+     KL          L+ + N      +
Sbjct: 860  VLEEMKISDC------PMF-------VFPTLSSVKKLEIWGEADAGGLSSISN----LST 919

Query: 958  LEDMSICKCPNVNSP-----PNLQDMQNLSSLIITNFQRLPEGLARLHNLKRLQVQGELR 1017
            L  + I     V S       NL+++  LS   + N + LP  LA L+NLK L ++    
Sbjct: 920  LTSLKIFSNHTVTSLLEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIR-YCY 961

Query: 1018 GFDWSPFTHLNSLESLVLLDMGSC--LSQLPQQLEHLIALRSLYI 1045
              +  P   L  L SL  L +  C  L  LP+ L+HL  L SL I
Sbjct: 980  ALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKI 961

BLAST of Spg005260 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.7e-120
Identity = 338/1009 (33.50%), Postives = 514/1009 (50.94%), Query Frame = 0

Query: 44   VEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQALKLWLEKL 103
            ++ LL         ++ L +GF+ E  +L++    ++A+L DA   +  ++ L+ WL+KL
Sbjct: 6    IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65

Query: 104  EDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANKIKAIAKKLD 163
                +E D +LDE   +  R           S +  +    + FR K+  ++  + KKL 
Sbjct: 66   NAATYEVDDILDEYKTKATR--------FSQSEYGRYHPKVIPFRHKVGKRMDQVMKKLK 125

Query: 164  EHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNKLI-DLSSQE 223
               + A       +  K VE    +  ET S L E  V GR+ E  EIV  LI ++S  +
Sbjct: 126  ---AIAEERKNFHLHEKIVERQAVR-RETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQ 185

Query: 224  ALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNAN 283
             L+VLPI+G+GGLGKT LA+ +F+ + + E F   IW+CVSE F  K +++AI+E+    
Sbjct: 186  HLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGR 245

Query: 284  --FGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKFGSVVVV 343
               G +D    + ++LQ+LL+ K+Y LVLDDVWNE+   W+ LRA +LK+    G+ V+ 
Sbjct: 246  PLLGEMDLAP-LQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRA-VLKVGAS-GASVLT 305

Query: 344  TTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLVKKFGGI 403
            TTR ++V  IM T   + L  L  + CW LF + AFG +  + P +  + + +VKK GG+
Sbjct: 306  TTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSGGV 365

Query: 404  PLVVKVLGGMV--KLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQC 463
            PL  K LGG++  K ++  +E ++ S   +  +P QDE+ IL  ++LS  +LP   LKQC
Sbjct: 366  PLAAKTLGGILCFKREERAWEHVRDS--PIWNLP-QDESSILPALRLSYHQLP-LDLKQC 425

Query: 464  FAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQDV-V 523
            FAYC+ FP+     KE L+ +W+A GF  L S  N+ +ED+G++ +  L  RS FQ++ V
Sbjct: 426  FAYCAVFPKDAKMEKEKLISLWMAHGF--LLSKGNMELEDVGDEVWKELYLRSFFQEIEV 485

Query: 524  RDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRTVHCSENV 583
            +D +    Y KMHDLIHD+A ++ +A     ++    K     ++SIG            
Sbjct: 486  KDGK---TYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGF----------- 545

Query: 584  AGKFHMSTSDSHVFHN--KITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHSRIR 643
                    ++   F+    +  FI LRVL        KLP+SI  L HLRYL++  S +R
Sbjct: 546  --------AEVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMR 605

Query: 644  ELPKSIVLLYNLQTLRLGSDT--TDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLI 703
             LPK +  L NLQTL L   T    LP +  KL +LR+L    + S     MP  +  L 
Sbjct: 606  SLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNL--LLDGSQSLTCMPPRIGSLT 665

Query: 704  QLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLY 763
             L+TL  FVVG  KG ++ ELG L  L G + +S LERVK+ ++A  ANL+ K NL  L 
Sbjct: 666  CLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLS 725

Query: 764  FQWSLRCEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLP---SGAFVENLVEIYLY 823
              W+           ++ VLE L+PH NL  LKI  F G  LP   + + ++N+V I + 
Sbjct: 726  MSWN-NFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILIS 785

Query: 824  DCKRCETLPMLGQLAKLEVLHIR----SLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQ 883
            + + C  LP  G L  LE L +      +  V+ +  + +  +   R  FP L+   I  
Sbjct: 786  NFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGF-PTRIRFPSLRKLDIWD 845

Query: 884  MNSLEHWEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRL--------FPS-- 943
              SL+   + E       FP LE + I  C  L    N  A  S R+        FP   
Sbjct: 846  FGSLKGLLKKE---GEEQFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFPEEM 905

Query: 944  FQGPPKLRSLKIVGCEKLTELPNWLELCHSLEDMSICKCPNVNSPP--NLQDMQNLSSLI 1003
            F+    L+ L I  C  L ELP  L   ++L+ + I  C  + S P   L+ + +L+ L 
Sbjct: 906  FKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELF 963

Query: 1004 ITN---FQRLPEGLARLHNLKRLQVQG---------ELRGFDWSPFTHL 1012
            + +    + LPEGL  L  L  L+++G         +  G DW   +H+
Sbjct: 966  VEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHI 963

BLAST of Spg005260 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 7.6e-118
Identity = 342/1088 (31.43%), Postives = 530/1088 (48.71%), Query Frame = 0

Query: 44   VEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQALKLWLEKL 103
            ++ LL+       +++ L +GF+KE  KL++    ++A+L DA   + + +A++ WL+KL
Sbjct: 6    LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65

Query: 104  EDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANKIKAIAKKLD 163
                +E D +L E   E +R           S    +    + FR K+  ++K I +KLD
Sbjct: 66   NSAAYEVDDILGECKNEAIR--------FEQSRLGFYHPGIINFRHKIGRRMKEIMEKLD 125

Query: 164  E-HYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNKLI-DLSSQ 223
                       L  +T ++     +   ET   L E  V GR+ E  EIV  LI +++  
Sbjct: 126  AISEERRKFHFLEKITERQA---AAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVA 185

Query: 224  EALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNA 283
            E L V PI+G+GGLGKT LA+ IF+ E + + F+  IWVCVS+ F  K +++ I+     
Sbjct: 186  EELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIER 245

Query: 284  NFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKFGSVVVVT 343
            +   ++      ++LQ+LL+ K+Y LVLDDVWN++   W++LRA +L +  + G+ ++ T
Sbjct: 246  SSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRA-VLTVGAR-GASILAT 305

Query: 344  TRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLVKKFGGIP 403
            TR ++V  IM T   +HL  L       LF + AFG +    P +  + + +VKK GG+P
Sbjct: 306  TRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCGGVP 365

Query: 404  LVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQCFAY 463
            L  K LGG+++  +   E        +  +P QDE+ IL  ++LS   LP   L+QCFAY
Sbjct: 366  LAAKTLGGLLRFKREESEWEHVRDNEIWSLP-QDESSILPALRLSYHHLP-LDLRQCFAY 425

Query: 464  CSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQDVVRDNR 523
            C+ FP+    IKE+L+ +W+A GF  L S  N+ +ED+G + +N L  RS FQ++  + +
Sbjct: 426  CAVFPKDTKMIKENLITLWMAHGF--LLSKGNLELEDVGNEVWNELYLRSFFQEI--EAK 485

Query: 524  GRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRTVHCSENVAGKF 583
                Y K+HDLIHD+A ++ +A     ++           +SIG        S ++  K 
Sbjct: 486  SGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVSSYSPSLLKK- 545

Query: 584  HMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHSRIRELPKSI 643
                            F+ LRVL      + +LP+SI  L HLRYLD+S +  R LP+ +
Sbjct: 546  ----------------FVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNFRSLPERL 605

Query: 644  VLLYNLQTLRLGS--DTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQTLS 703
              L NLQTL + +      LP +  KL +LRHL         T   P  +  L  L+TL 
Sbjct: 606  CKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTST---PPRIGLLTCLKTLG 665

Query: 704  SFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSLR 763
             F+VG  KG ++ EL  L  L G +S++ LERVK+  +A  ANL+ K NL  L   W   
Sbjct: 666  FFIVGSKKGYQLGELKNL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSWD-N 725

Query: 764  CEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPS---GAFVENLVEIYLYDCKRCE 823
                     ++ VLE L+PH NL+ L+I  F G   PS    + +E ++ + +  CK C 
Sbjct: 726  DGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCL 785

Query: 824  TLPMLGQLAKLEVLHIRS-LHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLEHWEE 883
             LP  G+L  LE L +++    V+ + ++   +    R  FP LK   I    SL+   +
Sbjct: 786  CLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMK 845

Query: 884  IETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEKLT 943
             E       FP LE ++IL+C      P F       +FP+     KL            
Sbjct: 846  EE---GEEKFPMLEEMAILYC------PLF-------VFPTLSSVKKL------------ 905

Query: 944  ELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNLKRLQ 1003
                                  V+   N + + ++S+L      R+        N +   
Sbjct: 906  ---------------------EVHGNTNTRGLSSISNLSTLTSLRIGA------NYRATS 965

Query: 1004 VQGELRGFDWSPFTHLNSLESLVLLDMGSCLSQLPQQLEHLIALRSLYIVCFNGVESLPE 1063
            +  E+       FT L +LE L   D  + L  LP  L  L AL+ L I   + +ES PE
Sbjct: 966  LPEEM-------FTSLTNLEFLSFFDFKN-LKDLPTSLTSLNALKRLQIESCDSLESFPE 986

Query: 1064 -WLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIEL---DEGGFEREK 1120
              L  + SL  L +  C+ LK LP  E +  LT L  LGV GC ++E     E G +  K
Sbjct: 1026 QGLEGLTSLTQLFVKYCKMLKCLP--EGLQHLTALTNLGVSGCPEVEKRCDKEIGEDWHK 986

BLAST of Spg005260 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 423.7 bits (1088), Expect = 6.5e-117
Identity = 325/1011 (32.15%), Postives = 519/1011 (51.34%), Query Frame = 0

Query: 44   VEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQALKLWLEKL 103
            ++ +L         ++ L +GF+ E  +L++    ++A+L DA   +   + L+ WL+KL
Sbjct: 6    IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65

Query: 104  EDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANKIKAIAKKLD 163
                +E D +LDE   +  R         + S +  +    + FR K+  ++  + KKL+
Sbjct: 66   NAATYEVDDILDEYKTKATR--------FLQSEYGRYHPKVIPFRHKVGKRMDQVMKKLN 125

Query: 164  EHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNKLIDLSSQ-E 223
               + A       +  K +E   +   ET S L E  V GR+ E  EIV  LI+ +S  +
Sbjct: 126  ---AIAEERKKFHLQEKIIERQ-AATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQ 185

Query: 224  ALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNA- 283
             L+VLPI+G+GGLGKT L++ +F+ + + E F   IW+C+S+ F  K +++AI+E+    
Sbjct: 186  KLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGK 245

Query: 284  NFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKFGSVVVVT 343
            +   +D    + ++LQ+LL+ K+YFLVLDDVWNE+ + W+ LRA +LK+    G+ V+ T
Sbjct: 246  SLSDMDLAP-LQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRA-VLKVGAS-GAFVLTT 305

Query: 344  TRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLVKKFGGIP 403
            TR ++V  IM T   + L  L  + CW LF + AFG +  + P +  + + +VKK GG+P
Sbjct: 306  TRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVP 365

Query: 404  LVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQCFAY 463
            L  K LGG+++  +   E        +  +P QDE+ IL  ++LS   LP   L+QCF Y
Sbjct: 366  LAAKTLGGILRFKREEREWEHVRDSPIWNLP-QDESSILPALRLSYHHLP-LDLRQCFVY 425

Query: 464  CSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQDVVRDNR 523
            C+ FP+     KE+L+  W+A GF  L S  N+ +ED+G + +N L  RS FQ++  ++ 
Sbjct: 426  CAVFPKDTKMAKENLIAFWMAHGF--LLSKGNLELEDVGNEVWNELYLRSFFQEIEVES- 485

Query: 524  GRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRTVHCSENVAGKF 583
            G+  Y KMHDLIHD+A ++ +A     ++     ++   ++SIG           V   +
Sbjct: 486  GK-TYFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGF--------AEVVSSY 545

Query: 584  HMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDIS-HSRIRELPKS 643
              S          +  F+ LRVL      + +LP+SI  L HLRYLD+S + RIR LPK 
Sbjct: 546  SPSL---------LQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKR 605

Query: 644  IVLLYNLQTLRLG--SDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQTL 703
            +  L NLQTL L      + LP +  KL +LR+L         T   P  +  L  L++L
Sbjct: 606  LCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTST---PPRIGLLTCLKSL 665

Query: 704  SSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSL 763
            S FV+G  KG ++ EL  L  L G +S++ L+RVK   +A  ANL+ K NL  L   W L
Sbjct: 666  SCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWDL 725

Query: 764  RCEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLP---SGAFVENLVEIYLYDCKRC 823
                +  +  D  VLE L+PH NL+ L+I  F G  LP   + + ++N+V I +  C+ C
Sbjct: 726  ----DGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENC 785

Query: 824  ETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLEHWEE 883
              LP  G+L  LE L    LH+  +  +    N H  R  FP L+   I   ++L+   +
Sbjct: 786  SCLPPFGELPCLESL---ELHTGSADVEYVEDNVHPGR--FPSLRKLVIWDFSNLKGLLK 845

Query: 884  IETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEKLT 943
            +E       FP LE ++  +C  +  IP   +  + ++  +      LRS+  +      
Sbjct: 846  ME---GEKQFPVLEEMTFYWC-PMFVIPTLSSVKTLKVIVT--DATVLRSISNLRALTSL 905

Query: 944  ELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNLKRLQ 1003
            ++ + +E     E+M            +L +++ L      N + LP  LA L+ LK L+
Sbjct: 906  DISDNVEATSLPEEMF----------KSLANLKYLKISFFRNLKELPTSLASLNALKSLK 948

Query: 1004 VQGELRGFDWSPFTHLNSLESLVLLDMGSC--LSQLPQQLEHLIALRSLYI 1045
             +      +  P   +  L SL  L + +C  L  LP+ L+HL AL +L I
Sbjct: 966  FE-FCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTI 948

BLAST of Spg005260 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 4.7e-107
Identity = 310/1014 (30.57%), Postives = 510/1014 (50.30%), Query Frame = 0

Query: 69   LSKLTNSLLMVEAILGDADRIKAEHQALKLWLEKLEDIVFEADVLLDELAYEDVRRKVET 128
            L +L+ +LL + A+L DA+  +  +  ++ W+ +L D+V+ A+  LD++A E +R  +  
Sbjct: 39   LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI-- 98

Query: 129  GKDLMVSNFVSFSKNPLVF-------RFKMANKIKAIAKKLDEHYSAASIVGLVAMTSKE 188
            G +   SN +   +  +            +  +++ +  +L+   S  +I+GL  +T+  
Sbjct: 99   GAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMI 158

Query: 189  VETDLSQILETDSFLDEIGVIGRETEVSEIVNKLIDLSSQE-ALAVLPIVGVGGLGKTAL 248
             +    Q L T S +DE  V GR+ +  EI+  LI  + ++  + V+ IVG+GG+GKT L
Sbjct: 159  PK----QRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTL 218

Query: 249  AKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNA---NFGGLDRKEIILQELQ 308
            ++ +++ + +R  F   +W  VSE F +  I + + E+  +    F  LD  ++ L+E +
Sbjct: 219  SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKE-R 278

Query: 309  KLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKFGSVVVVTTRSDEVAKIMETHHRH 368
               +   + LVLDD+WNEN   W  LR     I+   GS ++VTTRS  VA IM   H H
Sbjct: 279  LTGTGLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRVASIMCAVHVH 338

Query: 369  HLRKLPNDHCWSLFEKCAFGSELPVIPR-VDVVREMLVKKFGGIPLVVKVLGGMVKLDKS 428
            +L+ L +  CWSLF K  FG++ P + R +  + E +V K  G+PL VK LGG+++ +  
Sbjct: 339  NLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGK 398

Query: 429  NYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESL 488
              E  +     +  +P  D++++L V+++S   LP + LK+CFAYCS FP+G  F K+ +
Sbjct: 399  VIEWERVLSSRIWDLP-ADKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFEKDKV 458

Query: 489  VQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDI 548
            V +W+A+GF+Q  + S+  +E++G +YF+ L SRSL Q      + R I   MHD I+++
Sbjct: 459  VLLWMAEGFLQ-QTRSSKNLEELGNEYFSELESRSLLQ----KTKTRYI---MHDFINEL 518

Query: 549  ACAISNAQNLQLD----LSMDEKSWSDEVLSIGH------------KIRTVHCSENVAGK 608
            A   S   + + +    L + E++     L   +            K        ++   
Sbjct: 519  AQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNS 578

Query: 609  FHMSTSDSHVFHNKITDFIYLRVL-IAHAWFIRKLPNSIAKLKHLRYLDISHSRIRELPK 668
                  D  V    +     LRVL ++H    R  P+    + H R+LD+S + + +LPK
Sbjct: 579  SRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPK 638

Query: 669  SIVLLYNLQTLRLG--SDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQT 728
            S+  +YNLQTL L   S   +LPT +  L+NLR+L+    +  + +QMPR   RL  LQT
Sbjct: 639  SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDL---IGTKLRQMPRRFGRLKSLQT 698

Query: 729  LSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWS 788
            L++F V    G +I ELG L  L G+L +  L+RV    +A  ANL  K++L ++ F W 
Sbjct: 699  LTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWR 758

Query: 789  LRCEREDCN------YNDLNVLEGLQPHKNLQVLKIQNFAGELLP---SGAFVENLVEIY 848
                  + N       N+  V E L+PH++++ L I+ + G   P   S      +V I 
Sbjct: 759  TGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIR 818

Query: 849  LYDCKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGN----YHDERTLFPKLKTFHI 908
            L +C+ C +LP LGQL  L+ LHI  +  ++SIG +FY +       ++  F  L+T   
Sbjct: 819  LRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRF 878

Query: 909  SQMNSLEHWEEIETVSNGTTFPHLESLSILFCLKLM-NIPNFFASHSSRLFPSFQGPPKL 968
              +   + W ++  V+ G  FP L+ L IL C +L   +P F               P L
Sbjct: 879  DNLPDWQEWLDVR-VTRGDLFPSLKKLFILRCPELTGTLPTFL--------------PSL 938

Query: 969  RSLKIVGCEKLTELPNWLELCH-SLEDMSI-CKCPNVNSPPNLQDMQNLSSLIITNFQRL 1028
             SL I  C  L   P+  E  + +L+ +SI   C  +   P L    NL  L +      
Sbjct: 939  ISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKFP-LNHFANLDKLEVD----- 998

Query: 1029 PEGLARLHNLKRLQVQGE-LRGFDWSPFTHLNSLESLVLLDMGSCLSQLPQQLE 1035
                 +  +L  L++  E LRG +      +N  ++L LL     L+ LPQ L+
Sbjct: 999  -----QCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPK---LNALPQNLQ 1001

BLAST of Spg005260 vs. ExPASy TrEMBL
Match: A0A6J1CTV3 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014729 PE=4 SV=1)

HSP 1 Score: 1648.6 bits (4268), Expect = 0.0e+00
Identity = 839/1103 (76.07%), Postives = 953/1103 (86.40%), Query Frame = 0

Query: 27   VGQSTKRNMAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDA 86
            +G+S  RNMAEF+WTFAV+E+LKKTV+LAAEQ+G+AWGF KELSKL +SL+MVEAIL D 
Sbjct: 33   LGESQNRNMAEFLWTFAVQEVLKKTVRLAAEQIGMAWGFNKELSKLRDSLVMVEAILRDV 92

Query: 87   DRIKA--EHQALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGK-DLMVSN-FVSFSK 146
            DRIKA  EH ALKLW+EKL+DIVFEADVLLD++AYED+RRKVETGK  +MVSN F S SK
Sbjct: 93   DRIKAEPEHHALKLWVEKLQDIVFEADVLLDDIAYEDLRRKVETGKVQVMVSNCFASSSK 152

Query: 147  NPLVFRFKMANKIKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVI 206
            NP++FR KMA KIK IAK LD HY  AS VGLVA TSKE E DLSQI ETDSFLDEIGVI
Sbjct: 153  NPIIFRLKMARKIKVIAKMLDRHYYRASAVGLVARTSKETEPDLSQIQETDSFLDEIGVI 212

Query: 207  GRETEVSEIVNKLIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCV 266
            GRE+EVSEIVNKLI+LS+QEAL++LPIVG+ GLGKT+LAK IF+H +I+ +FD+ IWVCV
Sbjct: 213  GRESEVSEIVNKLIELSNQEALSILPIVGMPGLGKTSLAKVIFNHGLIKANFDEIIWVCV 272

Query: 267  SEPFVIKNILRAILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSE 326
            SEPFVI  ILR++LET N N GGL+ KE ILQELQKLL+ KKYFLV DDVWNENP+LWSE
Sbjct: 273  SEPFVINKILRSVLETLNPNSGGLESKEAILQELQKLLNAKKYFLVFDDVWNENPDLWSE 332

Query: 327  LRACLLKINKKFGSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPV 386
            LRACLLKI ++ G+V+VVTTRS+EVAKIMETHHRHHLRKL N+ CWSLFEKCAFG+ELP+
Sbjct: 333  LRACLLKITQESGNVLVVTTRSEEVAKIMETHHRHHLRKLSNNQCWSLFEKCAFGNELPI 392

Query: 387  IPRVDVVREMLVKKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSV 446
            IP++DVV+E L+KKFGGIPLVVKVLGG+VKL+K N EGLQS+LEN++RIPLQD++H+LS 
Sbjct: 393  IPKMDVVKEELIKKFGGIPLVVKVLGGIVKLEK-NPEGLQSTLENIMRIPLQDKSHVLST 452

Query: 447  IKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEK 506
            IKLSVDRLPS SLKQCFAYCS FP  FLF+KE+L+QMWIA+GFIQL +GSN++MEDIGEK
Sbjct: 453  IKLSVDRLPSPSLKQCFAYCSKFPPDFLFVKETLIQMWIARGFIQLSNGSNLMMEDIGEK 512

Query: 507  YFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDE-V 566
            YFNILLSRSLFQDVV+DNRGRII+CKMHDLIHD+ACAISNAQ+LQLDL +D K   D+ V
Sbjct: 513  YFNILLSRSLFQDVVKDNRGRIIHCKMHDLIHDVACAISNAQSLQLDLLIDGKRQRDKAV 572

Query: 567  LSIGHKIRTVHCSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKL 626
            LSIGH+IRT++CSEN  GKFH     SHVFHN+IT FIYLRVLI H+WFIRKL +SI KL
Sbjct: 573  LSIGHRIRTLYCSENGVGKFH-----SHVFHNRITSFIYLRVLIVHSWFIRKLSDSIVKL 632

Query: 627  KHLRYLDISHSRIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQ 686
            KHLRYLDISHS IRELPKSIVLLYNLQTL+LGSDT DLP  LRKLVNLRHLEF  N S+Q
Sbjct: 633  KHLRYLDISHSLIRELPKSIVLLYNLQTLKLGSDTVDLPKNLRKLVNLRHLEFPINPSNQ 692

Query: 687  TKQMPRHLSRLIQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAA 746
            TKQMPRHLSRLIQLQTLSSFVVG D+GCKIEELGPLK LRGELSL CLERVKS+EEAMAA
Sbjct: 693  TKQMPRHLSRLIQLQTLSSFVVGFDEGCKIEELGPLKNLRGELSLFCLERVKSREEAMAA 752

Query: 747  NLAEKENLSQLYFQWSLR-CEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAF 806
            NLAEKEN+S LY QW LR CEREDC+YND+NVLEGLQPH+NLQVLKIQNFAGELLP+  F
Sbjct: 753  NLAEKENISDLYLQWGLRSCEREDCHYNDMNVLEGLQPHRNLQVLKIQNFAGELLPNCIF 812

Query: 807  VENLVEIYLYDCKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLK 866
            VE LVEIYL+DCKRCETLPMLGQL+KLEVL IR LHSVKSIG EFYGNY  E TLFPKLK
Sbjct: 813  VEKLVEIYLHDCKRCETLPMLGQLSKLEVLRIRCLHSVKSIGHEFYGNYRHEMTLFPKLK 872

Query: 867  TFHISQMNSLEHWEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGP 926
            TFHI QMN+LEHWEEIETV NGT FP+LESL I+ CLKL N+PNFFAS S       +GP
Sbjct: 873  TFHIFQMNNLEHWEEIETVPNGTIFPYLESLKIVCCLKLRNVPNFFASDSE------EGP 932

Query: 927  PKLRSLKIVGCEKLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITN-FQ 986
             KL SL I  CE LT+LPN +E C SLE++ I KCPN+NSPPNLQ+M  LSSL I N   
Sbjct: 933  AKLLSLTIHECENLTKLPNGIEFCSSLENICISKCPNLNSPPNLQNMPKLSSLTIRNCIP 992

Query: 987  RLPEGLARLHNLKRLQVQGELRGFDWSPFTHLNSLESLVLLDMGSCLSQLPQQLEHLIAL 1046
            +L EGL  + NLK L++QG+ + +DWSP   L+SLE+L LL+MGSC +Q+PQQL+HL  L
Sbjct: 993  KLLEGLPCICNLKTLRIQGKWQEYDWSPMIQLSSLENLELLEMGSC-TQIPQQLQHLTTL 1052

Query: 1047 RSLYIVCFNGVESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQ 1106
            RSLYI   + +E+LPEWLGN+ SLE LNL+ CRNLKNLPSKEAMS LTRLNRL VHGCSQ
Sbjct: 1053 RSLYIGRCDSIETLPEWLGNLTSLEKLNLFGCRNLKNLPSKEAMSNLTRLNRLEVHGCSQ 1112

Query: 1107 IELDEGGFEREKVSHVPHIDFVP 1123
            IEL EGGFER KVSHVPHIDFVP
Sbjct: 1113 IELGEGGFERAKVSHVPHIDFVP 1122

BLAST of Spg005260 vs. ExPASy TrEMBL
Match: A0A0A0KTZ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G015850 PE=4 SV=1)

HSP 1 Score: 1549.6 bits (4011), Expect = 0.0e+00
Identity = 811/1099 (73.79%), Postives = 924/1099 (84.08%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MA+F+W+FAV+E+LKKTVKL AEQ+G++WGFKK+LSKL +SLLMVEAIL D +RIKAEHQ
Sbjct: 1    MADFLWSFAVDEVLKKTVKLVAEQIGMSWGFKKDLSKLRDSLLMVEAILRDVNRIKAEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            AL+LW+EKLE IVFEADVLLDEL+YED+RRKV+      V +FVS SKNPLVFR KMANK
Sbjct: 61   ALRLWVEKLEHIVFEADVLLDELSYEDLRRKVDARP---VRSFVSSSKNPLVFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            IKAIAK+LDEHY AASI+GLVA+TSKEVE++ SQILETDSFLDEIGVIGRE EV EIVNK
Sbjct: 121  IKAIAKRLDEHYCAASIMGLVAITSKEVESEPSQILETDSFLDEIGVIGREAEVLEIVNK 180

Query: 215  LIDLSSQE-ALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILR 274
            L++LS QE AL+VLPIVG+GGLGKT+LAK IFHHEMIRE+FD+ IWVCVSEPFVI  ILR
Sbjct: 181  LLELSKQEAALSVLPIVGIGGLGKTSLAKAIFHHEMIRENFDRMIWVCVSEPFVINKILR 240

Query: 275  AILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKK 334
            AILET NANFGGLD KE +LQELQKLL +KKYFLVLDDVWNENP+LW+ELRACLLK NKK
Sbjct: 241  AILETLNANFGGLDNKEALLQELQKLLRNKKYFLVLDDVWNENPDLWNELRACLLKANKK 300

Query: 335  FGSVVVVTTRSDEVAKIMETHH-RHHLRKLPNDHCWSLFEKCAFGSELPVIPRVD-VVRE 394
            FGSV+VVTTRSDEVA I+ET+H RH LRKL ND+CW+LFEKCAFGS+LPV PRVD V+RE
Sbjct: 301  FGSVIVVTTRSDEVANIVETNHQRHRLRKLSNDYCWTLFEKCAFGSDLPVTPRVDHVIRE 360

Query: 395  MLVKKFGGIPLVVKVLGGMVKLDKSN-YEGLQSSLENLIRIPLQDENHILSVIKLSVDRL 454
             LVK+FGGIPLVVKV GGMVKLDK+   +GL+S+LENLI  PLQ EN ILS IKLSVDRL
Sbjct: 361  ELVKRFGGIPLVVKVFGGMVKLDKNKCCQGLRSTLENLIISPLQYENSILSTIKLSVDRL 420

Query: 455  PSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSR 514
            PSSSLKQCFAYCSNFPRGFLFI+E LVQMWIAQGFI LPSGSNV MEDIG  YFN LLSR
Sbjct: 421  PSSSLKQCFAYCSNFPRGFLFIREPLVQMWIAQGFIHLPSGSNVTMEDIGANYFNTLLSR 480

Query: 515  SLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRT 574
            SLFQDVV+D+R RI+YCKMHD++HD+ACAISNAQ L+L      KS  D+ LSIGH+IRT
Sbjct: 481  SLFQDVVKDDRERILYCKMHDVVHDVACAISNAQKLRL----SGKSNGDKALSIGHEIRT 540

Query: 575  VHCSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDIS 634
            +HCSENV  +FH+ T DSHVFHN+I++F YL VLI H+WFI +LP+SIAKLKHLRYLDIS
Sbjct: 541  LHCSENVVERFHLPTFDSHVFHNEISNFTYLCVLIIHSWFIHQLPDSIAKLKHLRYLDIS 600

Query: 635  HSRIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLS 694
            HS IR LP SIV LYNLQTLRLGS    LPTKLRKLVNLRHLEF  +LS QTKQMP+HLS
Sbjct: 601  HSLIRTLPDSIVSLYNLQTLRLGSKIMHLPTKLRKLVNLRHLEF--SLSTQTKQMPQHLS 660

Query: 695  RLIQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLS 754
            RL+QLQTLSSFVVG DKGCKIEELGPL  L+GELSL  LE VKSK EAMAANLA KEN+S
Sbjct: 661  RLLQLQTLSSFVVGFDKGCKIEELGPLNNLKGELSLFHLEHVKSKTEAMAANLAMKENIS 720

Query: 755  QLYFQWSLRCEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLY 814
             LYFQWSL  EREDC+ NDLNVLEGL+PHKNLQ LKI+NF G +LP+G FVENLVE+ LY
Sbjct: 721  DLYFQWSLLSEREDCSNNDLNVLEGLRPHKNLQALKIENFGG-VLPNGLFVENLVEVILY 780

Query: 815  DCKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGN---YHDE--RTLFPKLKTFHIS 874
            DCKRCETLPMLG L+KLE+LHIR L SVKSIGDEFYGN   YH+E    LFPKLKT HIS
Sbjct: 781  DCKRCETLPMLGHLSKLELLHIRCLDSVKSIGDEFYGNNNSYHNEWSSLLFPKLKTLHIS 840

Query: 875  QMNSLEHWEEIETVSN-GTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLR 934
            QM SLE W+EI + SN G TFPHLESLSI++C KLMNIPN            FQ PPKL+
Sbjct: 841  QMKSLELWQEIGSSSNYGATFPHLESLSIVWCSKLMNIPNL-----------FQVPPKLQ 900

Query: 935  SLKIVGCEKLTELPNWLELCHSLEDMSICKCPNV--NSPPNLQDMQNLSSLIITNFQRLP 994
            SLKI  CEKLT+LP+WL LC S+E+M IC CPNV  NS PNL+ M NLSSL I  F++LP
Sbjct: 901  SLKIFYCEKLTKLPHWLNLCSSIENMVICNCPNVNNNSLPNLKSMPNLSSLSIQAFEKLP 960

Query: 995  EGLARLHNLKRLQVQGELRGFDWSPFTHLN-SLESLVLLDMG--SCLSQLPQQLEHLIAL 1054
            EGLA +HNLKRL V GEL+G DWSPF +LN S+E L L++ G  + L QLP+QLE+L AL
Sbjct: 961  EGLATIHNLKRLDVYGELQGLDWSPFMYLNSSIEILRLVNTGVSNLLLQLPRQLEYLTAL 1020

Query: 1055 RSLYIVCFNGVESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQ 1114
            RSL I  F+ ++SLPEWLGN+ SLETLNL  C+NLK+ PS EAMS LT+L+RL  + C Q
Sbjct: 1021 RSLDIERFSDIDSLPEWLGNLTSLETLNLRYCKNLKSFPSIEAMSNLTKLSRLETYECFQ 1078

Query: 1115 IELDEGGFEREKVSHVPHI 1119
            ++LDEG +ER K++HV  I
Sbjct: 1081 LKLDEGSYERAKIAHVHDI 1078

BLAST of Spg005260 vs. ExPASy TrEMBL
Match: A0A6J1CUL2 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014727 PE=4 SV=1)

HSP 1 Score: 1520.4 bits (3935), Expect = 0.0e+00
Identity = 770/1083 (71.10%), Postives = 896/1083 (82.73%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MAEF+WTFA +ELLK+ VKLAAEQ  LAWGF KEL KL +SLLMVEAIL D DRIK EHQ
Sbjct: 1    MAEFLWTFAAQELLKQAVKLAAEQTALAWGFNKELLKLRDSLLMVEAILRDVDRIKGEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            A+KLW+EKL++IVFEADVLLD+LAYE +RRKVE G + MVSNFVS SKNPL FR KMANK
Sbjct: 61   AVKLWVEKLQEIVFEADVLLDDLAYEHLRRKVEAGNEAMVSNFVSISKNPLAFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            I+AIA+ LD+HYS+A+ VGLVA+T KE+E D SQI ETDSFLDE GVIGRE EVSEIVN+
Sbjct: 121  IRAIARMLDKHYSSAAAVGLVAVTPKEIEPDFSQIQETDSFLDEFGVIGREIEVSEIVNR 180

Query: 215  LIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRA 274
            LID S ++AL+VLPIVG+GGLGKTAL K +F+HE I+ +FD+TIWVCVSEPFVIK ILRA
Sbjct: 181  LIDFSHRDALSVLPIVGMGGLGKTALMKVVFNHEFIKGNFDRTIWVCVSEPFVIKKILRA 240

Query: 275  ILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKF 334
            ILET N NFGGLD KE +LQELQKLL+DK+ FLVLDDVWNEN  LW+EL+ACLLKI+++ 
Sbjct: 241  ILETLNPNFGGLDSKEALLQELQKLLNDKRCFLVLDDVWNENLTLWNELKACLLKISQRS 300

Query: 335  GSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLV 394
            G+ VVVTTRSD+VA++METH RHHL+ L +DHCWSLF+   FG+E   I  +D+V+  LV
Sbjct: 301  GNTVVVTTRSDKVAEVMETHPRHHLKSLSDDHCWSLFKSYVFGNESLDIQELDIVKGELV 360

Query: 395  KKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSS 454
            K+FGGIPLVVKVLGG VK D+ N EGLQS+LENL+RIPL+ ENH+LS IKLSVDRLPSSS
Sbjct: 361  KRFGGIPLVVKVLGGTVKFDE-NSEGLQSTLENLMRIPLEGENHVLSTIKLSVDRLPSSS 420

Query: 455  LKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ 514
            LKQCFAYCSNFP+ F F K++L+QMWIAQGFIQ   GSNV MED+G+KYFN+LLSRSLFQ
Sbjct: 421  LKQCFAYCSNFPQDFEFRKQALIQMWIAQGFIQSLGGSNVTMEDVGDKYFNVLLSRSLFQ 480

Query: 515  DVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDL--SMDEKSWSDEVLSIGHKIRTVH 574
            DVV+D+RGRIIYCKMHDLIHD+AC ISNAQ L+L+L  S+D      E  SIGH+IRT+ 
Sbjct: 481  DVVKDSRGRIIYCKMHDLIHDVACVISNAQKLKLNLLDSLDGIRRRGEDRSIGHRIRTLD 540

Query: 575  CSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHS 634
            CSEN A K H+   DSHVFHNKI DF+YLRVLIAH+WFI KLP+S+AKLKHLRY+DISHS
Sbjct: 541  CSENDARKLHVLAFDSHVFHNKILDFVYLRVLIAHSWFINKLPDSVAKLKHLRYIDISHS 600

Query: 635  RIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRL 694
             IRELP+SIVLLYNLQTLRLGS   DLP  LRKLV+LRHLE F N  + T QMP+ L +L
Sbjct: 601  TIRELPESIVLLYNLQTLRLGSFLKDLPKNLRKLVSLRHLEIFTNPCYST-QMPQRLGQL 660

Query: 695  IQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQL 754
             QLQTLSSFVVG D+GC+IEELG LK L+G+LSL  LERVKSK+EAM A L EK+N+S L
Sbjct: 661  SQLQTLSSFVVGFDEGCRIEELGTLKNLKGKLSLLRLERVKSKKEAMTAKLVEKDNISDL 720

Query: 755  YFQWSLRCEREDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYDC 814
            YFQW L  ER+  NYNDLNVLEGLQPHKNLQVL+I NFAGELLP+G FVE LVEIYL+ C
Sbjct: 721  YFQWCLIGERQGSNYNDLNVLEGLQPHKNLQVLRIHNFAGELLPNGTFVEKLVEIYLHGC 780

Query: 815  KRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLEH 874
            +RC+TLPMLGQL+KLEVL IR LHSV+SIG+EFYGNY D+RTLF KL+TFHIS+M+SLEH
Sbjct: 781  ERCKTLPMLGQLSKLEVLEIRDLHSVRSIGEEFYGNYRDKRTLFHKLRTFHISEMSSLEH 840

Query: 875  WEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCE 934
            WEEI  V NGT FPHLESLSI+ CL+L +IPNFFASH         G  KLRSLKI+GCE
Sbjct: 841  WEEIRAVPNGTIFPHLESLSIVCCLRLTSIPNFFASHEEERL----GSAKLRSLKILGCE 900

Query: 935  KLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNLK 994
             LTELPN LE C SL+ M I  C N+NS P+LQ+M NLSSL IT FQ+LPEGLA++ NLK
Sbjct: 901  NLTELPNGLEFCSSLKTMWIGNCSNLNSVPSLQNMHNLSSLSITEFQKLPEGLAQVRNLK 960

Query: 995  RLQVQGELRGFDWSPFTHLNSLESLVLLDMGSCLS-QLPQQLEHLIALRSLYIVCFNGVE 1054
             L V G L+G+DWSP  HL SLE+LVL D G   + QLPQQLEHL  LRSL+I  F+GVE
Sbjct: 961  SLSVHGYLQGYDWSPLVHLGSLENLVLADFGGTAAIQLPQQLEHLTNLRSLHISHFDGVE 1020

Query: 1055 SLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFEREK 1114
            +LPEW  N  SLE L +Y+CR LKN+PSKEAMSKLTRL  L V+GCS++E+ +  FER K
Sbjct: 1021 ALPEWFRNFTSLEILKVYNCRKLKNMPSKEAMSKLTRLKSLRVYGCSKLEVGQCDFERVK 1077

BLAST of Spg005260 vs. ExPASy TrEMBL
Match: A0A5A7TSS6 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003620 PE=4 SV=1)

HSP 1 Score: 1490.7 bits (3858), Expect = 0.0e+00
Identity = 754/1088 (69.30%), Postives = 900/1088 (82.72%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MAEF+WTFA +ELLKKTVKLAAEQ+GLAWGF  ELS L +SLLMVEAIL D DRIKAEHQ
Sbjct: 1    MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            A+KLW+EKLE IVFE DVLLDELAYED+RRKVE+ K+ +VSNF+SFSK PLVFR KMANK
Sbjct: 61   AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            IK IAK L++HYSAAS VGLVA+ SK+ E D SQI ETDSFLDE GVIGRETEV EIVN 
Sbjct: 121  IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 215  LIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRA 274
             +DLS +E L+VLPIVG+GGLGKTALAK IF+HE+I+ +FD+T+WVCVSEPF+IK ILRA
Sbjct: 181  SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 275  ILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKF 334
            ILET N+NFGGLD KE +LQELQKLL+DKKYFLVLDDVWNENP LW+EL+ CLLKI+++ 
Sbjct: 241  ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 335  GSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLV 394
            G+VVVVTTRSD VA+IMETH R+HL KL +DHCWSLF+K AFG+EL  IP +D+V++ LV
Sbjct: 301  GNVVVVTTRSDRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKELV 360

Query: 395  KKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSS 454
            K+FGGIPL VKV+GG+VK D+ N+EGLQ SLENL+R+ LQDENH++S IKL+VDRLP SS
Sbjct: 361  KRFGGIPLAVKVMGGIVKFDE-NHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLSS 420

Query: 455  LKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ 514
            LKQCFAYCSNFP+ F F +E+L+QMWIAQGFIQ    S+ +MEDIGEKYFN+LLSR LFQ
Sbjct: 421  LKQCFAYCSNFPKDFKFRREALIQMWIAQGFIQPSLESDEMMEDIGEKYFNVLLSRFLFQ 480

Query: 515  DVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLS--MDEKSWSDEVLSIGHKIRTVH 574
            D+V+DNRGRII+CKMHDLIHD+ACAISN+Q L+ D S  +D + W  +  S  H+++T  
Sbjct: 481  DIVKDNRGRIIFCKMHDLIHDVACAISNSQGLKWDPSDLLDGEPWRRQACSASHELKTPD 540

Query: 575  CSENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHS 634
            C+EN + K HM T DSHVFHNK+TDFI+LRVLIAH+WFI KLPNSIAKLKHLRYLDIS+S
Sbjct: 541  CNENHSRKLHMLTFDSHVFHNKVTDFIHLRVLIAHSWFICKLPNSIAKLKHLRYLDISYS 600

Query: 635  RIRELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRL 694
             IRELP S+VLLYNLQTL+L     DLP  LRKLV+LRHLEFF++  + TKQMP+HL +L
Sbjct: 601  TIRELPDSVVLLYNLQTLKLSRFFNDLPKNLRKLVSLRHLEFFSDPCN-TKQMPQHLGKL 660

Query: 695  IQLQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQL 754
            IQLQTLSSFVVG D GCKIEEL  L+ L+G L+L CLERVKSK EAMAANL EK N+S L
Sbjct: 661  IQLQTLSSFVVGFDDGCKIEELRSLRNLKGNLNLLCLERVKSKMEAMAANLVEKGNISFL 720

Query: 755  YFQWSLRCER-EDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYD 814
            YF W+LR ER E  NYNDLNVLEGLQPHKNLQ L+I+NF G+LLP+  FVENLVEIYL++
Sbjct: 721  YFYWTLRSERSEGSNYNDLNVLEGLQPHKNLQALRIRNFLGKLLPNVIFVENLVEIYLHE 780

Query: 815  CKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLE 874
            C+ CETLP LGQL+KLEVL +R L SV+SIG+EFYGNY ++R LFP LKTFHI +M +LE
Sbjct: 781  CEICETLPTLGQLSKLEVLELRCLCSVRSIGEEFYGNYREKRILFPTLKTFHICEMINLE 840

Query: 875  HWEEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGC 934
            +WEEI  VSNGT F +LESL I+ C +L +IPN FA H    FPSFQ   KLRSLKI+GC
Sbjct: 841  NWEEIMVVSNGTIFSNLESLYIVCCPRLTSIPNLFAYHHESSFPSFQLSAKLRSLKILGC 900

Query: 935  EKLTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNL 994
            E L + PN LE C SLE+M I  C N+N PP+L++MQNL+SL IT F++LP+GLA++  L
Sbjct: 901  ESLQKQPNGLEFCSSLENMWISNCSNLNYPPSLRNMQNLTSLSITEFRKLPDGLAQVCKL 960

Query: 995  KRLQVQGELRGFDWSPFTHLNSLESLVLLDM-GSCLSQLPQQLEHLIALRSLYIVCFNGV 1054
            + L V G L+G+DWSP  HL+SLE+LVL+D+ GS   QLP QLE L +LRSL++  F+G+
Sbjct: 961  RSLSVHGYLQGYDWSPLVHLDSLENLVLVDLEGSGAIQLPHQLEQLTSLRSLHLSHFSGI 1020

Query: 1055 ESLPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFERE 1114
            E+LPEW GN  SLE L LY+C NLKN+PSKEA+SKLTRL  L V+GC Q++LD G FER 
Sbjct: 1021 EALPEWFGNFTSLEILKLYNCVNLKNMPSKEAISKLTRLTSLRVYGCPQLKLDGGDFERV 1080

Query: 1115 KVSHVPHI 1119
             +S VP +
Sbjct: 1081 NISLVPTV 1086

BLAST of Spg005260 vs. ExPASy TrEMBL
Match: A0A5D3CZ17 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001180 PE=4 SV=1)

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 752/1086 (69.24%), Postives = 893/1086 (82.23%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MAEF+WTFA +ELLKKTVKLAAEQ+GLAWGF  ELS L +SLLMVEAIL D DRIKAEHQ
Sbjct: 1    MAEFLWTFAAQELLKKTVKLAAEQIGLAWGFNNELSNLRDSLLMVEAILRDVDRIKAEHQ 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
            A+KLW+EKLE IVFE DVLLDELAYED+RRKVE+ K+ +VSNF+SFSK PLVFR KMANK
Sbjct: 61   AVKLWVEKLEAIVFEVDVLLDELAYEDLRRKVESQKEAIVSNFISFSKTPLVFRLKMANK 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETDSFLDEIGVIGRETEVSEIVNK 214
            IK IAK L++HYSAAS VGLVA+ SK+ E D SQI ETDSFLDE GVIGRETEV EIVN 
Sbjct: 121  IKNIAKMLEKHYSAASTVGLVAILSKQTEPDFSQIQETDSFLDEHGVIGRETEVLEIVNV 180

Query: 215  LIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRA 274
             +DLS +E L+VLPIVG+GGLGKTALAK IF+HE+I+ +FD+T+WVCVSEPF+IK ILRA
Sbjct: 181  SVDLSYREGLSVLPIVGMGGLGKTALAKVIFNHELIKGNFDRTVWVCVSEPFLIKKILRA 240

Query: 275  ILETFNANFGGLDRKEIILQELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKF 334
            ILET N+NFGGLD KE +LQELQKLL+DKKYFLVLDDVWNENP LW+EL+ CLLKI+++ 
Sbjct: 241  ILETLNSNFGGLDSKEALLQELQKLLNDKKYFLVLDDVWNENPILWNELKGCLLKISQRS 300

Query: 335  GSVVVVTTRSDEVAKIMETHHRHHLRKLPNDHCWSLFEKCAFGSELPVIPRVDVVREMLV 394
            G+VVVVTTRSD VA+IMETH R+HL KL +DHCWSLF+K AFG+EL  IP +D+V++ LV
Sbjct: 301  GNVVVVTTRSDRVAEIMETHSRYHLTKLSDDHCWSLFKKYAFGNELLGIPELDIVQKELV 360

Query: 395  KKFGGIPLVVKVLGGMVKLDKSNYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSS 454
            K+FGGIPL VKV+GG+VK D+ N+EGLQ SLENL+R+ LQDENH++S IKL+VDRLP SS
Sbjct: 361  KRFGGIPLAVKVMGGIVKFDE-NHEGLQKSLENLMRLQLQDENHVVSTIKLTVDRLPLSS 420

Query: 455  LKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ 514
            LKQCFAYCSNFP+ F F +E+LVQMWIAQGFIQ   GS+ +MEDIGEKYFN+LLSR LFQ
Sbjct: 421  LKQCFAYCSNFPKDFKFRREALVQMWIAQGFIQPSLGSDEMMEDIGEKYFNVLLSRFLFQ 480

Query: 515  DVVRDNRGRIIYCKMHDLIHDIACAISNAQNLQLDLSMDEKSWSDEVLSIGHKIRTVHCS 574
            D+V+DNRGRII+CKMHDLIHD+ACAISN+Q L+         W    L  G +++T  C+
Sbjct: 481  DIVKDNRGRIIFCKMHDLIHDVACAISNSQGLK---------WDPSDLLDG-ELKTPDCN 540

Query: 575  ENVAGKFHMSTSDSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHSRI 634
            EN + K HM T DSHVFHNK+TDFI+LRVLIAH+WFI KLPNSIAKLKHLRYLDIS+S I
Sbjct: 541  ENHSRKLHMLTFDSHVFHNKVTDFIHLRVLIAHSWFICKLPNSIAKLKHLRYLDISYSTI 600

Query: 635  RELPKSIVLLYNLQTLRLGSDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQ 694
            RELP S+VLLYNLQTL+L     DLP  LRKLV+LRHLEFF++  + TKQMP+HL +LIQ
Sbjct: 601  RELPDSVVLLYNLQTLKLSRFFNDLPKNLRKLVSLRHLEFFSDPCN-TKQMPQHLGKLIQ 660

Query: 695  LQTLSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYF 754
            LQTLS FVVG D GCKIEEL  L+ L+G L+L CLERVKSK EAMAANL EK N+S LYF
Sbjct: 661  LQTLSGFVVGFDDGCKIEELRSLRNLKGNLNLLCLERVKSKMEAMAANLVEKGNISFLYF 720

Query: 755  QWSLRCER-EDCNYNDLNVLEGLQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYDCK 814
             W+LR ER E  NYNDLNVLEGLQPHKNLQ L+I+NF G+LLP+  FVENLVEIYL++C+
Sbjct: 721  YWTLRSERSEGSNYNDLNVLEGLQPHKNLQALRIRNFLGKLLPNVIFVENLVEIYLHECE 780

Query: 815  RCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGNYHDERTLFPKLKTFHISQMNSLEHW 874
             CETLP LGQL+KLEVL +R L SV+SIG+EFYGNY ++R LFP LKTFHI +M +LE+W
Sbjct: 781  ICETLPTLGQLSKLEVLELRCLCSVRSIGEEFYGNYREKRILFPTLKTFHICEMINLENW 840

Query: 875  EEIETVSNGTTFPHLESLSILFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEK 934
            EEI  VSNGT F +LESL+I+ C +L +IPN FA H    FPSFQ   KLRSLKI+GCE 
Sbjct: 841  EEIMVVSNGTIFSNLESLNIVCCPRLTSIPNLFAYHHESSFPSFQLSAKLRSLKILGCES 900

Query: 935  LTELPNWLELCHSLEDMSICKCPNVNSPPNLQDMQNLSSLIITNFQRLPEGLARLHNLKR 994
            L + PN LE C SLE+M I  C N+N PP+L++MQNL+SL IT F++LP+GL ++  L+ 
Sbjct: 901  LQKQPNGLEFCSSLENMWISNCSNLNYPPSLRNMQNLTSLSITEFRKLPDGLGQVCKLRS 960

Query: 995  LQVQGELRGFDWSPFTHLNSLESLVLLDM-GSCLSQLPQQLEHLIALRSLYIVCFNGVES 1054
            L V G L+G+DWSP  HL SLE+LVL+D+ GS   QLP QLE L +LRSL++  F+G+E+
Sbjct: 961  LSVHGYLQGYDWSPLVHLGSLENLVLVDLEGSGAIQLPHQLEQLTSLRSLHLSHFSGIEA 1020

Query: 1055 LPEWLGNVASLETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFEREKV 1114
            LPEW GN  SLE L LY+C NLKN+PSKEA+SKLTRL  L V+GC Q++LD G FER  +
Sbjct: 1021 LPEWFGNFTSLEILKLYNCVNLKNMPSKEAISKLTRLTSLRVYGCPQLKLDGGDFERVNI 1074

Query: 1115 SHVPHI 1119
            S VP I
Sbjct: 1081 SLVPTI 1074

BLAST of Spg005260 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 391.0 bits (1003), Expect = 3.3e-108
Identity = 310/1014 (30.57%), Postives = 510/1014 (50.30%), Query Frame = 0

Query: 69   LSKLTNSLLMVEAILGDADRIKAEHQALKLWLEKLEDIVFEADVLLDELAYEDVRRKVET 128
            L +L+ +LL + A+L DA+  +  +  ++ W+ +L D+V+ A+  LD++A E +R  +  
Sbjct: 39   LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI-- 98

Query: 129  GKDLMVSNFVSFSKNPLVF-------RFKMANKIKAIAKKLDEHYSAASIVGLVAMTSKE 188
            G +   SN +   +  +            +  +++ +  +L+   S  +I+GL  +T+  
Sbjct: 99   GAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMI 158

Query: 189  VETDLSQILETDSFLDEIGVIGRETEVSEIVNKLIDLSSQE-ALAVLPIVGVGGLGKTAL 248
             +    Q L T S +DE  V GR+ +  EI+  LI  + ++  + V+ IVG+GG+GKT L
Sbjct: 159  PK----QRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTL 218

Query: 249  AKEIFHHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNA---NFGGLDRKEIILQELQ 308
            ++ +++ + +R  F   +W  VSE F +  I + + E+  +    F  LD  ++ L+E +
Sbjct: 219  SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKE-R 278

Query: 309  KLLSDKKYFLVLDDVWNENPNLWSELRACLLKINKKFGSVVVVTTRSDEVAKIMETHHRH 368
               +   + LVLDD+WNEN   W  LR     I+   GS ++VTTRS  VA IM   H H
Sbjct: 279  LTGTGLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRVASIMCAVHVH 338

Query: 369  HLRKLPNDHCWSLFEKCAFGSELPVIPR-VDVVREMLVKKFGGIPLVVKVLGGMVKLDKS 428
            +L+ L +  CWSLF K  FG++ P + R +  + E +V K  G+PL VK LGG+++ +  
Sbjct: 339  NLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGK 398

Query: 429  NYEGLQSSLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESL 488
              E  +     +  +P  D++++L V+++S   LP + LK+CFAYCS FP+G  F K+ +
Sbjct: 399  VIEWERVLSSRIWDLP-ADKSNLLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFEKDKV 458

Query: 489  VQMWIAQGFIQLPSGSNVVMEDIGEKYFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDI 548
            V +W+A+GF+Q  + S+  +E++G +YF+ L SRSL Q      + R I   MHD I+++
Sbjct: 459  VLLWMAEGFLQ-QTRSSKNLEELGNEYFSELESRSLLQ----KTKTRYI---MHDFINEL 518

Query: 549  ACAISNAQNLQLD----LSMDEKSWSDEVLSIGH------------KIRTVHCSENVAGK 608
            A   S   + + +    L + E++     L   +            K        ++   
Sbjct: 519  AQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNS 578

Query: 609  FHMSTSDSHVFHNKITDFIYLRVL-IAHAWFIRKLPNSIAKLKHLRYLDISHSRIRELPK 668
                  D  V    +     LRVL ++H    R  P+    + H R+LD+S + + +LPK
Sbjct: 579  SRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPK 638

Query: 669  SIVLLYNLQTLRLG--SDTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQT 728
            S+  +YNLQTL L   S   +LPT +  L+NLR+L+    +  + +QMPR   RL  LQT
Sbjct: 639  SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDL---IGTKLRQMPRRFGRLKSLQT 698

Query: 729  LSSFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWS 788
            L++F V    G +I ELG L  L G+L +  L+RV    +A  ANL  K++L ++ F W 
Sbjct: 699  LTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWR 758

Query: 789  LRCEREDCN------YNDLNVLEGLQPHKNLQVLKIQNFAGELLP---SGAFVENLVEIY 848
                  + N       N+  V E L+PH++++ L I+ + G   P   S      +V I 
Sbjct: 759  TGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIR 818

Query: 849  LYDCKRCETLPMLGQLAKLEVLHIRSLHSVKSIGDEFYGN----YHDERTLFPKLKTFHI 908
            L +C+ C +LP LGQL  L+ LHI  +  ++SIG +FY +       ++  F  L+T   
Sbjct: 819  LRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRF 878

Query: 909  SQMNSLEHWEEIETVSNGTTFPHLESLSILFCLKLM-NIPNFFASHSSRLFPSFQGPPKL 968
              +   + W ++  V+ G  FP L+ L IL C +L   +P F               P L
Sbjct: 879  DNLPDWQEWLDVR-VTRGDLFPSLKKLFILRCPELTGTLPTFL--------------PSL 938

Query: 969  RSLKIVGCEKLTELPNWLELCH-SLEDMSI-CKCPNVNSPPNLQDMQNLSSLIITNFQRL 1028
             SL I  C  L   P+  E  + +L+ +SI   C  +   P L    NL  L +      
Sbjct: 939  ISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKFP-LNHFANLDKLEVD----- 998

Query: 1029 PEGLARLHNLKRLQVQGE-LRGFDWSPFTHLNSLESLVLLDMGSCLSQLPQQLE 1035
                 +  +L  L++  E LRG +      +N  ++L LL     L+ LPQ L+
Sbjct: 999  -----QCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPK---LNALPQNLQ 1001

BLAST of Spg005260 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 310.5 bits (794), Expect = 5.7e-84
Identity = 250/872 (28.67%), Postives = 418/872 (47.94%), Query Frame = 0

Query: 69  LSKLTNSLLMVEAILGDADRIKAEH-QALKLWLEKLEDIVFEADVLLDELAYEDVRRKVE 128
           L +L  +L+    +L DAD+ +AEH + +K WL  ++D  F+A+ +LDEL  E +RR+V 
Sbjct: 36  LKRLKVALVTANPVLADADQ-RAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVV 95

Query: 129 TGKDLMVSNFVSFSKNPLVFRFKMANKIKAIAKKLDEHYSAASIVGLVAMTSKEVETDLS 188
                +   F +        + K+  K++ + + L+ H     ++GL   +         
Sbjct: 96  AEAGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQ 155

Query: 189 QILETDSFLDEIGVIGRETEVSEIVNKLI--DLSSQEALAVLPIVGVGGLGKTALAKEIF 248
                   L +  ++GR  +   +VN L+  D  S    AV+ +VG+ G+GKT L + +F
Sbjct: 156 ASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVF 215

Query: 249 HHEMIRESFDKTIWVCVSEPFVIKNILRAILETFNANFGGLDRKEIILQELQKLLSDKKY 308
           +   + E F+  +W+     F +  + +A+L+   ++    +    +  +L+K LS K++
Sbjct: 216 NDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRF 275

Query: 309 FLVLDDVWNENPNLWSELRACLLKINKKFGSVVVVTTRSDEVAKIMETHHRHHLRKLPND 368
            LVLDD W+E+ + W   +       +  GS +V+TTRS+ V+ + +    + ++ + N+
Sbjct: 276 LLVLDDFWSESDSEWESFQVAFTDAEE--GSKIVLTTRSEIVSTVAKAEKIYQMKLMTNE 335

Query: 369 HCWSLFEKCAFG--SELPVIPRVDVVREMLVKKFGGIPLVVKVLGGMVKLDKSNYEGLQS 428
            CW L  + AFG  S   +   ++ + + + ++  G+PL  + +   ++  K N +   +
Sbjct: 336 ECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLR-SKPNPDDWYA 395

Query: 429 SLENLIRIPLQDENHILSVIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQ 488
             +N         N IL V+KLS D LP   LK+CFA CS FP+G +F +E LV +W+A 
Sbjct: 396 VSKNFSSY----TNSILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFDREELVLLWMAI 455

Query: 489 GFIQLPSGSNVVMEDIGEKYFNILLSRSLFQ--DVVRDNRGRIIYCKMHDLIHDIACAIS 548
             +  P  S   +EDIG  Y   L+++S FQ  D+   +        MHDL++D+A A+S
Sbjct: 456 DLLYQPRSSR-RLEDIGNDYLGDLVAQSFFQRLDITMTS------FVMHDLMNDLAKAVS 515

Query: 549 NAQNLQL-DLSMDEKSWSDEVLSIGHKIRTVHCSENVAGK-------------FHMSTS- 608
                +L D ++ E   +    S         C  +VA +             F+  TS 
Sbjct: 516 GDFCFRLEDDNIPEIPSTTRHFSFSRS----QCDASVAFRSICGAEFLRTILPFNSPTSL 575

Query: 609 -----DSHVFHNKITDFIYLRVLIAHAWFIRKLPNSIAKLKHLRYLDISHSRIRELPKSI 668
                   V +  +     LR+L    + I  LP S+  LK LRYLD+S ++I+ELP+ +
Sbjct: 576 ESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFV 635

Query: 669 VLLYNLQTLRLGS--DTTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQTLS 728
             L NLQTL L +  D T LP  + +L+NLR L+    +     +MP  + +L  LQ LS
Sbjct: 636 CTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDL---VGTPLVEMPPGIKKLRSLQKLS 695

Query: 729 SFVVGCDKGCKIEELGPLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSLR 788
           +FV+G   G  + EL  L  LRG L +S L+ V    EA  A L  K  L  L  +W+++
Sbjct: 696 NFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVK 755

Query: 789 CER-EDCNYNDL-----NVLEGLQPHKNLQVLKIQNFAGELLP---SGAFVENLVEIYLY 848
                  ++N L      VL  L+PH +L+   I+++ G   P     +    +  + L 
Sbjct: 756 GSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLS 815

Query: 849 DCKRCETLPMLGQLAKLEVLHIRSLHSVKSIG-DEFYGNYHDERTLFPKLKTFHISQMNS 900
            C  C +LP +GQL  L+ L I   + ++ +G D F+G  +     F  L+      M  
Sbjct: 816 SCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPR 875

BLAST of Spg005260 vs. TAIR 10
Match: AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 243.8 bits (621), Expect = 6.5e-64
Identity = 275/1137 (24.19%), Postives = 508/1137 (44.68%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MA  + +F ++ L      L +++  L  G + ++++L   L ++ + L DAD  K    
Sbjct: 1    MAGELISFGIQNLW----NLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSA 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
             +K  +E++++I+++ +  ++    E    K  +G    +        +   +   +   
Sbjct: 61   VVKNCVEEIKEIIYDGEDTIETFVLEQNLGKT-SGIKKSIRRLACIIPDRRRYALGIGGL 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETD-SFLDEIGVIGRETEVSEIVN 214
               I+K + +  S      +V    K+ + D  + +    S  D+   +G E  V ++V 
Sbjct: 121  SNRISKVIRDMQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVG 180

Query: 215  KLIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILR 274
             L+D   +  + V+ I G+GGLGKT LAK++F+HE ++  FD   WVCVS+ F   N+ +
Sbjct: 181  YLVD---EANVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQ 240

Query: 275  AILETFNANFGG---LDRKEIILQ-ELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLK 334
             IL            ++  +  LQ EL +LL   K  +VLDD+W +    W EL   +  
Sbjct: 241  KILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIWEKED--W-ELIKPIFP 300

Query: 335  INKKFGSVVVVTTRSDEVAKIMETHHRHHLRK-LPNDHCWSLFEKCAF----GSELPVIP 394
              K  G  V++T+R++ VA    T + +   + L  +  W+LF++ A      +E  +  
Sbjct: 301  PTK--GWKVLLTSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDE 360

Query: 395  RVDVVREMLVKKFGGIPLVVKVLGGMV--KLDKSNYEGLQSSLENLI---RIPLQDENHI 454
              + + ++++K  GG+PL ++VLGGM+  K    ++  L  ++ + +   R    D+N+ 
Sbjct: 361  EKEELGKLMIKHCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNN 420

Query: 455  LS--VIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVME 514
                V+ LS + LP S LK CF Y ++FP  +    ++L   W A+G  Q       ++ 
Sbjct: 421  TCNYVLSLSFEELP-SYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIR 480

Query: 515  DIGEKYFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQN-LQLDLSMDEKS 574
            D+G+ Y   L+ R++          R   C +HD++ ++    +  +N LQ+  S     
Sbjct: 481  DVGDVYIEELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTSTG 540

Query: 575  WSDEVLSIGHKIRTVHCSENVAGKFH-------MSTSDSHVF---------HNKITDFIY 634
             S  +++    +     + +V    +       +  +++++F          +       
Sbjct: 541  NSLSIVTSRRLVYQYPITLDVEKDINDPKLRSLVVVANTYMFWGGWSWMLLGSSFIRLEL 600

Query: 635  LRVLIAHAWFIR--KLPNSIAKLKHLRYLDISHSRIRELPKS---IVLLYNLQTLRLGSD 694
            LRVL  H   ++  KL +SI +L HLRYL++ H+ +  +P S   + LL  L  + L S 
Sbjct: 601  LRVLDIHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSG 660

Query: 695  TTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQTLSSFVVGCDKGCKIEELG 754
            +T +P  L+++  LR+L    ++  +TK     LS L++L+TL +F     K C +E+L 
Sbjct: 661  STLVPNVLKEMQQLRYLALPKDMGRKTK---LELSNLVKLETLKNF---STKNCSLEDLR 720

Query: 755  PLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSLRCEREDCNYNDLNVLEG 814
             + +LR   +L+   R ++  E +AA++   + L  L    ++     +    +  ++  
Sbjct: 721  GMVRLR---TLTIELRKETSLETLAASIGGLKYLESL----TITDLGSEMRTKEAGIVFD 780

Query: 815  LQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYDCKRCE-TLPMLGQLAKLEVLHIRS 874
                  L+ L ++ +   L     F  +L  +YL  C+  E  +P+L +L +L+ L +R 
Sbjct: 781  F---VYLKTLTLKLYMPRLSKEQHFPSHLTTLYLQHCRLEEDPMPILEKLHQLKELELRR 840

Query: 875  LHSVKSI-GDEFYGNYHDERTLFPKLKTFHISQMNSLEHWEEIETVSNGTTFPHLESLSI 934
                KS  G E   +       FP+L+   I  +   E W+  E     ++ P L +L I
Sbjct: 841  ----KSFSGKEMVCSSGG----FPQLQKLSIKGLEEWEDWKVEE-----SSMPVLHTLDI 900

Query: 935  LFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEKLTELPNWLELCHSLEDMSIC 994
              C KL  +P+    H          P  L S+ +  C            C   + M   
Sbjct: 901  RDCRKLKQLPD---EHL---------PSHLTSISLFFC------------CLEEDPM--- 960

Query: 995  KCPNVNSPPNLQDMQNLSSLIITNFQR-----LPEGLARLHNLKRLQVQGELRGFDWSPF 1054
              P +    +L+++Q    L+  +F          G  +LH LK  ++ G     +W   
Sbjct: 961  --PTLERLVHLKELQ----LLFRSFSGRIMVCAGSGFPQLHKLKLSELDGL---EEW--I 1020

Query: 1055 THLNSLESLVLLDMGSC--LSQLPQQLEHLIALRSLYIVCFNGVESLPEWL---GNVASL 1114
                S+  L  L++  C  L +LP     L  L        N +E   EW+   G++  L
Sbjct: 1021 VEDGSMPQLHTLEIRRCPKLKKLPNGFPQLQNLE------LNELEEWEEWIVEDGSMPLL 1048

Query: 1115 ETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFEREKVSHVPHIDF 1121
             TL +++C  LK LP  + +  +  L  L V    +  L +GG +  KV H+P ++F
Sbjct: 1081 HTLRIWNCPKLKQLP--DGLRFIYSLKNLTVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

BLAST of Spg005260 vs. TAIR 10
Match: AT1G59218.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 243.8 bits (621), Expect = 6.5e-64
Identity = 275/1137 (24.19%), Postives = 508/1137 (44.68%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MA  + +F ++ L      L +++  L  G + ++++L   L ++ + L DAD  K    
Sbjct: 1    MAGELISFGIQNLW----NLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSA 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
             +K  +E++++I+++ +  ++    E    K  +G    +        +   +   +   
Sbjct: 61   VVKNCVEEIKEIIYDGEDTIETFVLEQNLGKT-SGIKKSIRRLACIIPDRRRYALGIGGL 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETD-SFLDEIGVIGRETEVSEIVN 214
               I+K + +  S      +V    K+ + D  + +    S  D+   +G E  V ++V 
Sbjct: 121  SNRISKVIRDMQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVG 180

Query: 215  KLIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILR 274
             L+D   +  + V+ I G+GGLGKT LAK++F+HE ++  FD   WVCVS+ F   N+ +
Sbjct: 181  YLVD---EANVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQ 240

Query: 275  AILETFNANFGG---LDRKEIILQ-ELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLK 334
             IL            ++  +  LQ EL +LL   K  +VLDD+W +    W EL   +  
Sbjct: 241  KILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIWEKED--W-ELIKPIFP 300

Query: 335  INKKFGSVVVVTTRSDEVAKIMETHHRHHLRK-LPNDHCWSLFEKCAF----GSELPVIP 394
              K  G  V++T+R++ VA    T + +   + L  +  W+LF++ A      +E  +  
Sbjct: 301  PTK--GWKVLLTSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDE 360

Query: 395  RVDVVREMLVKKFGGIPLVVKVLGGMV--KLDKSNYEGLQSSLENLI---RIPLQDENHI 454
              + + ++++K  GG+PL ++VLGGM+  K    ++  L  ++ + +   R    D+N+ 
Sbjct: 361  EKEELGKLMIKHCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNN 420

Query: 455  LS--VIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVME 514
                V+ LS + LP S LK CF Y ++FP  +    ++L   W A+G  Q       ++ 
Sbjct: 421  TCNYVLSLSFEELP-SYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIR 480

Query: 515  DIGEKYFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQN-LQLDLSMDEKS 574
            D+G+ Y   L+ R++          R   C +HD++ ++    +  +N LQ+  S     
Sbjct: 481  DVGDVYIEELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTSTG 540

Query: 575  WSDEVLSIGHKIRTVHCSENVAGKFH-------MSTSDSHVF---------HNKITDFIY 634
             S  +++    +     + +V    +       +  +++++F          +       
Sbjct: 541  NSLSIVTSRRLVYQYPITLDVEKDINDPKLRSLVVVANTYMFWGGWSWMLLGSSFIRLEL 600

Query: 635  LRVLIAHAWFIR--KLPNSIAKLKHLRYLDISHSRIRELPKS---IVLLYNLQTLRLGSD 694
            LRVL  H   ++  KL +SI +L HLRYL++ H+ +  +P S   + LL  L  + L S 
Sbjct: 601  LRVLDIHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSG 660

Query: 695  TTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQTLSSFVVGCDKGCKIEELG 754
            +T +P  L+++  LR+L    ++  +TK     LS L++L+TL +F     K C +E+L 
Sbjct: 661  STLVPNVLKEMQQLRYLALPKDMGRKTK---LELSNLVKLETLKNF---STKNCSLEDLR 720

Query: 755  PLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSLRCEREDCNYNDLNVLEG 814
             + +LR   +L+   R ++  E +AA++   + L  L    ++     +    +  ++  
Sbjct: 721  GMVRLR---TLTIELRKETSLETLAASIGGLKYLESL----TITDLGSEMRTKEAGIVFD 780

Query: 815  LQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYDCKRCE-TLPMLGQLAKLEVLHIRS 874
                  L+ L ++ +   L     F  +L  +YL  C+  E  +P+L +L +L+ L +R 
Sbjct: 781  F---VYLKTLTLKLYMPRLSKEQHFPSHLTTLYLQHCRLEEDPMPILEKLHQLKELELRR 840

Query: 875  LHSVKSI-GDEFYGNYHDERTLFPKLKTFHISQMNSLEHWEEIETVSNGTTFPHLESLSI 934
                KS  G E   +       FP+L+   I  +   E W+  E     ++ P L +L I
Sbjct: 841  ----KSFSGKEMVCSSGG----FPQLQKLSIKGLEEWEDWKVEE-----SSMPVLHTLDI 900

Query: 935  LFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEKLTELPNWLELCHSLEDMSIC 994
              C KL  +P+    H          P  L S+ +  C            C   + M   
Sbjct: 901  RDCRKLKQLPD---EHL---------PSHLTSISLFFC------------CLEEDPM--- 960

Query: 995  KCPNVNSPPNLQDMQNLSSLIITNFQR-----LPEGLARLHNLKRLQVQGELRGFDWSPF 1054
              P +    +L+++Q    L+  +F          G  +LH LK  ++ G     +W   
Sbjct: 961  --PTLERLVHLKELQ----LLFRSFSGRIMVCAGSGFPQLHKLKLSELDGL---EEW--I 1020

Query: 1055 THLNSLESLVLLDMGSC--LSQLPQQLEHLIALRSLYIVCFNGVESLPEWL---GNVASL 1114
                S+  L  L++  C  L +LP     L  L        N +E   EW+   G++  L
Sbjct: 1021 VEDGSMPQLHTLEIRRCPKLKKLPNGFPQLQNLE------LNELEEWEEWIVEDGSMPLL 1048

Query: 1115 ETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFEREKVSHVPHIDF 1121
             TL +++C  LK LP  + +  +  L  L V    +  L +GG +  KV H+P ++F
Sbjct: 1081 HTLRIWNCPKLKQLP--DGLRFIYSLKNLTVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

BLAST of Spg005260 vs. TAIR 10
Match: AT1G58848.2 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 243.8 bits (621), Expect = 6.5e-64
Identity = 275/1137 (24.19%), Postives = 508/1137 (44.68%), Query Frame = 0

Query: 35   MAEFMWTFAVEELLKKTVKLAAEQMGLAWGFKKELSKLTNSLLMVEAILGDADRIKAEHQ 94
            MA  + +F ++ L      L +++  L  G + ++++L   L ++ + L DAD  K    
Sbjct: 1    MAGELISFGIQNLW----NLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSA 60

Query: 95   ALKLWLEKLEDIVFEADVLLDELAYEDVRRKVETGKDLMVSNFVSFSKNPLVFRFKMANK 154
             +K  +E++++I+++ +  ++    E    K  +G    +        +   +   +   
Sbjct: 61   VVKNCVEEIKEIIYDGEDTIETFVLEQNLGKT-SGIKKSIRRLACIIPDRRRYALGIGGL 120

Query: 155  IKAIAKKLDEHYSAASIVGLVAMTSKEVETDLSQILETD-SFLDEIGVIGRETEVSEIVN 214
               I+K + +  S      +V    K+ + D  + +    S  D+   +G E  V ++V 
Sbjct: 121  SNRISKVIRDMQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVG 180

Query: 215  KLIDLSSQEALAVLPIVGVGGLGKTALAKEIFHHEMIRESFDKTIWVCVSEPFVIKNILR 274
             L+D   +  + V+ I G+GGLGKT LAK++F+HE ++  FD   WVCVS+ F   N+ +
Sbjct: 181  YLVD---EANVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQ 240

Query: 275  AILETFNANFGG---LDRKEIILQ-ELQKLLSDKKYFLVLDDVWNENPNLWSELRACLLK 334
             IL            ++  +  LQ EL +LL   K  +VLDD+W +    W EL   +  
Sbjct: 241  KILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIWEKED--W-ELIKPIFP 300

Query: 335  INKKFGSVVVVTTRSDEVAKIMETHHRHHLRK-LPNDHCWSLFEKCAF----GSELPVIP 394
              K  G  V++T+R++ VA    T + +   + L  +  W+LF++ A      +E  +  
Sbjct: 301  PTK--GWKVLLTSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDE 360

Query: 395  RVDVVREMLVKKFGGIPLVVKVLGGMV--KLDKSNYEGLQSSLENLI---RIPLQDENHI 454
              + + ++++K  GG+PL ++VLGGM+  K    ++  L  ++ + +   R    D+N+ 
Sbjct: 361  EKEELGKLMIKHCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNN 420

Query: 455  LS--VIKLSVDRLPSSSLKQCFAYCSNFPRGFLFIKESLVQMWIAQGFIQLPSGSNVVME 514
                V+ LS + LP S LK CF Y ++FP  +    ++L   W A+G  Q       ++ 
Sbjct: 421  TCNYVLSLSFEELP-SYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIR 480

Query: 515  DIGEKYFNILLSRSLFQDVVRDNRGRIIYCKMHDLIHDIACAISNAQN-LQLDLSMDEKS 574
            D+G+ Y   L+ R++          R   C +HD++ ++    +  +N LQ+  S     
Sbjct: 481  DVGDVYIEELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSSRTSTG 540

Query: 575  WSDEVLSIGHKIRTVHCSENVAGKFH-------MSTSDSHVF---------HNKITDFIY 634
             S  +++    +     + +V    +       +  +++++F          +       
Sbjct: 541  NSLSIVTSRRLVYQYPITLDVEKDINDPKLRSLVVVANTYMFWGGWSWMLLGSSFIRLEL 600

Query: 635  LRVLIAHAWFIR--KLPNSIAKLKHLRYLDISHSRIRELPKS---IVLLYNLQTLRLGSD 694
            LRVL  H   ++  KL +SI +L HLRYL++ H+ +  +P S   + LL  L  + L S 
Sbjct: 601  LRVLDIHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYSLGNLKLLIYLNLVILVSG 660

Query: 695  TTDLPTKLRKLVNLRHLEFFANLSHQTKQMPRHLSRLIQLQTLSSFVVGCDKGCKIEELG 754
            +T +P  L+++  LR+L    ++  +TK     LS L++L+TL +F     K C +E+L 
Sbjct: 661  STLVPNVLKEMQQLRYLALPKDMGRKTK---LELSNLVKLETLKNF---STKNCSLEDLR 720

Query: 755  PLKKLRGELSLSCLERVKSKEEAMAANLAEKENLSQLYFQWSLRCEREDCNYNDLNVLEG 814
             + +LR   +L+   R ++  E +AA++   + L  L    ++     +    +  ++  
Sbjct: 721  GMVRLR---TLTIELRKETSLETLAASIGGLKYLESL----TITDLGSEMRTKEAGIVFD 780

Query: 815  LQPHKNLQVLKIQNFAGELLPSGAFVENLVEIYLYDCKRCE-TLPMLGQLAKLEVLHIRS 874
                  L+ L ++ +   L     F  +L  +YL  C+  E  +P+L +L +L+ L +R 
Sbjct: 781  F---VYLKTLTLKLYMPRLSKEQHFPSHLTTLYLQHCRLEEDPMPILEKLHQLKELELRR 840

Query: 875  LHSVKSI-GDEFYGNYHDERTLFPKLKTFHISQMNSLEHWEEIETVSNGTTFPHLESLSI 934
                KS  G E   +       FP+L+   I  +   E W+  E     ++ P L +L I
Sbjct: 841  ----KSFSGKEMVCSSGG----FPQLQKLSIKGLEEWEDWKVEE-----SSMPVLHTLDI 900

Query: 935  LFCLKLMNIPNFFASHSSRLFPSFQGPPKLRSLKIVGCEKLTELPNWLELCHSLEDMSIC 994
              C KL  +P+    H          P  L S+ +  C            C   + M   
Sbjct: 901  RDCRKLKQLPD---EHL---------PSHLTSISLFFC------------CLEEDPM--- 960

Query: 995  KCPNVNSPPNLQDMQNLSSLIITNFQR-----LPEGLARLHNLKRLQVQGELRGFDWSPF 1054
              P +    +L+++Q    L+  +F          G  +LH LK  ++ G     +W   
Sbjct: 961  --PTLERLVHLKELQ----LLFRSFSGRIMVCAGSGFPQLHKLKLSELDGL---EEW--I 1020

Query: 1055 THLNSLESLVLLDMGSC--LSQLPQQLEHLIALRSLYIVCFNGVESLPEWL---GNVASL 1114
                S+  L  L++  C  L +LP     L  L        N +E   EW+   G++  L
Sbjct: 1021 VEDGSMPQLHTLEIRRCPKLKKLPNGFPQLQNLE------LNELEEWEEWIVEDGSMPLL 1048

Query: 1115 ETLNLYSCRNLKNLPSKEAMSKLTRLNRLGVHGCSQIELDEGGFEREKVSHVPHIDF 1121
             TL +++C  LK LP  + +  +  L  L V    +  L +GG +  KV H+P ++F
Sbjct: 1081 HTLRIWNCPKLKQLP--DGLRFIYSLKNLTVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048