Homology
BLAST of Spg005214 vs. NCBI nr
Match:
XP_038877313.1 (putative disease resistance protein RGA3 [Benincasa hispida])
HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 692/1063 (65.10%), Postives = 823/1063 (77.42%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLW FAVQEVLKK VK+AAE+I +AWG +KELSKL LLK+EA LRDI++++LHHE
Sbjct: 124 MAEFLWIFAVQEVLKKTVKVAAEEISMAWGFKKELSKLRKCLLKSEAILRDINKKKLHHE 183
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SVRMWV++LQH VYE DDLLDELVYE LRQKV+TEK +KVR FFS S+N F FR KM K
Sbjct: 184 SVRMWVEDLQHFVYEVDDLLDELVYEHLRQKVDTEKRNKKVRNFFSSSNNPFPFRRKMAK 243
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETNKNI-LKQIRETTSTLNSE--VIGREAE 180
++K++TETL + Y E S G+VAL+G+ES ET+KNI LKQIRETTS LN E +IGRE E
Sbjct: 244 RMKDITETLYKHYCEASPLGLVALLGEESIETHKNINLKQIRETTSNLNFEEVIIGREVE 303
Query: 181 VSNIVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFI 240
VSNIVK+ I+SSNE+PMSIIPIVGMGG+G+TTLAKM+FNHEL+K HFDETIWVCVSEPFI
Sbjct: 304 VSNIVKMVIESSNEHPMSIIPIVGMGGLGETTLAKMVFNHELVKDHFDETIWVCVSEPFI 363
Query: 241 ITNILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCL 300
I NILE +F SLT TS+G +KENLL+ LQE+MQGKSY LVLD VWNEEA LWGEL++CL
Sbjct: 364 ILNILEGMFQSLTKTSSGL-NKENLLRKLQEEMQGKSYLLVLDYVWNEEARLWGELRECL 423
Query: 301 KESAGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELK 360
K AGK GN IIVTTRS +VA +METT SHH+ KLSDDQCWSLFKESA ANGL M+P+L+
Sbjct: 424 KNIAGKFGNSIIVTTRSAEVATVMETTSSHHLTKLSDDQCWSLFKESASANGLSMSPKLE 483
Query: 361 TIRVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSV 420
I+ ++++I GVPL+AKVLGGA FEGN+ERW+T+V+ VV NIS DDK+FV S +KLSV
Sbjct: 484 IIQKLVIRRIGGVPLVAKVLGGATKFEGNYERWLTQVESVVTNISNDDKDFVFSTLKLSV 543
Query: 421 DRLPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNF 480
D LPL S KQCFAYCSNFPKD+ F KE+LIQMWIAQGF+Q Q+G++ M+ E+ GE+Y NF
Sbjct: 544 DCLPLFSLKQCFAYCSNFPKDYEFAKETLIQMWIAQGFIQAQKGNNTMLEENIGEDYINF 603
Query: 481 LLSRSLFEVV---IKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLIC 540
LLSRSLF+ V I G +T F MHDLIHDIAC IS+HQ E +N EKS KLRT +
Sbjct: 604 LLSRSLFQDVSKDIDGTVTHFKMHDLIHDIACVISSHQNFEFKHSNWSEKSTRKLRTTVI 663
Query: 541 GTGYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDVES 600
K+ H+ L LRVLV+++ + + IGM KHLRYL DI G LK ES
Sbjct: 664 NN--KMIHDKLVDLGSLRVLVMNS-PFLRILPDSIGMLKHLRYL---DIIGSSILKLPES 723
Query: 601 IDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYEAS 660
I LYNLQTLKLG R+ LPKNLRKLV LRHLQF I K+MPL +SQLIHLQTL A+
Sbjct: 724 ITMLYNLQTLKLGTRVDLPKNLRKLVMLRHLQFFYIYIKQMPLCISQLIHLQTLPAFVVG 783
Query: 661 YEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKG 720
E+G KIEELGPL NLKGSL+L +L +VKSK+EAI A+L+ KKNL +L W+ S
Sbjct: 784 SEKGCKIEELGPLNNLKGSLQLSSLNRVKSKKEAIAAKLMGKKNLCELTFEWDVYS---- 843
Query: 721 NHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVK 780
E+ +NC N FEVL+GLQPH NL+SLS+ Y GQV PN IFVE LV
Sbjct: 844 ----------EEEKHNNC--NNDFEVLEGLQPHKNLRSLSVVHYGGQVLPNGIFVENLVI 903
Query: 781 IFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISL 840
IFL++C +CEMLPMLGQLPNLEEL ISS+ SVRSIG EFYG+ +NQRVSFPKLK++S
Sbjct: 904 IFLQECNRCEMLPMLGQLPNLEELHISSMGSVRSIGSEFYGNTHYSNQRVSFPKLKELSF 963
Query: 841 HFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPNQL 900
+QNLEQWED+++GIAFP+L+ +SI C KL+ VPH FVSL+TL + GC+EL +LPN L
Sbjct: 964 RLMQNLEQWEDVTDGIAFPYLQSLSIVICSKLLDVPHHFVSLRTLRIVGCEELAKLPNGL 1023
Query: 901 EHYNSIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSI-TDLSLTDSL 960
NSI+SLKIS+CPNL+LN+ N NL+EMEI GCMQDYDFSP H SI T LSL DSL
Sbjct: 1024 NCPNSIESLKISECPNLALNLHNMHNLKEMEIVGCMQDYDFSPLLHQSSILTHLSLIDSL 1083
Query: 961 ENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFASLEALHLSDCKNLKRLPSKEV 1020
NATELP+QL++LT LK L+I+ FN +EALP+WLGN SLE L L DCKNLK PSKEV
Sbjct: 1084 -RNATELPRQLKYLTALKALTIKNFNSLEALPDWLGNLTSLETLGLFDCKNLKCFPSKEV 1143
Query: 1021 MLCLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLPKICDDLK 1057
MLCL KLN L+V GCP+ L L Q PE AKLSHLP C D++
Sbjct: 1144 MLCLKKLNHLHV-RGCPM-LLLGQDDPEKAKLSHLPNNCVDVR 1160
BLAST of Spg005214 vs. NCBI nr
Match:
XP_022145229.1 (putative disease resistance protein RGA4 [Momordica charantia])
HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 670/1059 (63.27%), Postives = 797/1059 (75.26%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAVQEVLKK++KLAAEQIGVAW L+KELSKL LLKAE L DI+R++L HE
Sbjct: 1 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+WV++LQH VY+ADDLLD+LVYEDLRQKVE EK KVR F SPS+N FLFR+KM K
Sbjct: 61 SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKI-NKVRNFLSPSNNPFLFRFKMAK 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETN-KNILKQIRETTSTLNSEVIGREAEVS 180
++K ++ETL + Y E S V+ VG+ESTET KNIL+QIRETTS LN +VIGRE EV
Sbjct: 121 RMKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVL 180
Query: 181 NIVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIIT 240
I+KL IDSS+E+ MSIIPIVGMGG+GKTTLAKM+FNHELIK HFD+TIWVCVS+PFII
Sbjct: 181 KIMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIV 240
Query: 241 NILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKE 300
NILE IF SLTNTS+G KE L LQ++MQGK YFLVLDDVWNEE +LW EL+DCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300
Query: 301 SAGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTI 360
A KSGN IIVTTRS VA IM+T SH + KLSDD+CWSLFKESA ANGLLMNPEL+ I
Sbjct: 301 IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360
Query: 361 RVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDR 420
R +VK+I G+PL+AKVLGG FEGN+ERWVT V+ +V+NI D+K+FVLS +KLSVD
Sbjct: 361 REVVVKRIGGIPLVAKVLGGTAKFEGNYERWVTTVESIVRNILNDEKDFVLSTLKLSVDS 420
Query: 421 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 480
LPLLS KQCFA+CSNFPKD F KE+LIQMWIAQGF+Q +EGD+ ++ E+ GE YFNFLL
Sbjct: 421 LPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFNFLL 480
Query: 481 SRSLFEVV---IKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGT 540
SRSLF+ V I G IT F MHDLIHD+AC +SN QK+E N EK V LRTL+
Sbjct: 481 SRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRALRTLVINN 540
Query: 541 GYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDVESID 600
G + H L+ LRVLV+++ + KN IGM KHLRYL DI G + ESI
Sbjct: 541 G-AIIHNKLEDSVYLRVLVLNS-DFIKNLPDSIGMLKHLRYL---DIIGSRIRRLPESIT 600
Query: 601 KLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYEASYE 660
LYNLQ LKL RI LPKNLRKLV LRHL F I +KMPLH+SQLIHLQTL Y +E
Sbjct: 601 MLYNLQILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYIVGFE 660
Query: 661 QGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKGNH 720
+G KIEELGPL +LKGSL+LLNL +VKSKEEAI A+LIEK NL +L L H
Sbjct: 661 KGRKIEELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTL-----------H 720
Query: 721 WNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIF 780
WN+SS GEE N + N EVL+GLQPH NLQSLSI + G PN +FVE LV I
Sbjct: 721 WNSSSCGEEGYN-----NRNDSEVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAIV 780
Query: 781 LKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISLHF 840
L++C KCEMLPMLGQLPNLEEL +S + SVR IG EFYG+ ++ +++ FPKL+++S
Sbjct: 781 LQECNKCEMLPMLGQLPNLEELRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFSH 840
Query: 841 LQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVP-HCFVSLQTLVLEGCKELRELPNQLE 900
++ LEQWE++SNGI FPHLE +S+ C KL SVP H FVSL+TL + GC+EL +LP++LE
Sbjct: 841 MEKLEQWEELSNGINFPHLETLSMFYCSKLTSVPDHEFVSLRTLRIVGCEELSKLPSKLE 900
Query: 901 HYNSIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSITDLSLTDSLEN 960
N +KIS CP L LN+ N NL E+EI G M++YDFS H S+T++ LTDSL
Sbjct: 901 CCN---FMKISGCPKLRLNMHNMCNLNELEINGPMEEYDFSFLMHQSSVTNIFLTDSL-R 960
Query: 961 NATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFASLEALHLSDCKNLKRLPSKEVML 1020
NAT+LPQQLQHLTTLK+L+I FNG+EALPEWL A+LE + + +CKNLKRLPS++ ML
Sbjct: 961 NATQLPQQLQHLTTLKSLAIEYFNGVEALPEWLEKLANLETMGVFECKNLKRLPSQQAML 1020
Query: 1021 CLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLPKICDD 1055
LTKL L V GCPL L L QG E AKLSHLP D+
Sbjct: 1021 RLTKLKHLRV-RGCPL-LPLGQGDLERAKLSHLPITVDN 1027
BLAST of Spg005214 vs. NCBI nr
Match:
XP_038895409.1 (disease resistance protein RGA2-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 673/1059 (63.55%), Postives = 781/1059 (73.75%), Query Frame = 0
Query: 3 EFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESV 62
EFLWTF VQE LKK+VK+AA++I VAWG +KEL KL+ LLK+EA LRDI ++L++E V
Sbjct: 5 EFLWTFVVQEALKKVVKVAAQEIAVAWGFKKELEKLTKWLLKSEAILRDIITKKLYNEPV 64
Query: 63 RMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKL 122
RMWV++LQH VYEADDLLDELVYEDLR+K ETEK ++KV FFS S N FLFR KM K++
Sbjct: 65 RMWVEDLQHFVYEADDLLDELVYEDLRRKAETEKIEKKVHNFFSCSHNPFLFRRKMAKRM 124
Query: 123 KEVTETLCQLYHETSSFGVVALVGQESTETNKNI-LKQIRETTSTLNSE--VIGREAEVS 182
K++TETL + Y E S G+VALVG ESTE KNI LKQIR+TTS L E +IGRE EVS
Sbjct: 125 KDITETLYKHYCEASPLGLVALVGHESTEARKNIDLKQIRDTTSKLKPEEVIIGREVEVS 184
Query: 183 NIVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIIT 242
NIVKL I+SSNE+PMSIIPIVGMGG+GKTTLAKM+FNH L++ HFD+T+WVCVSEPFII
Sbjct: 185 NIVKLVIESSNEHPMSIIPIVGMGGLGKTTLAKMVFNHGLVQTHFDKTLWVCVSEPFIII 244
Query: 243 NILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKE 302
NILE IF SLT TS+ +KENLL+ LQE+M+G +YFLVLDDVWNEEA LW LKDCL E
Sbjct: 245 NILEGIFQSLTKTSSSCLNKENLLQRLQEEMRGNNYFLVLDDVWNEEAILWDNLKDCLNE 304
Query: 303 SAGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTI 362
AGK GN IIVTTRS VA +M TT S H+ KLSDD CWSLFK+ A+GL EL+ I
Sbjct: 305 IAGKPGNTIIVTTRSPDVATVMGTTSSIHLKKLSDDLCWSLFKKGVSADGL----ELEII 364
Query: 363 RVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDR 422
R E+V KI G+PL+AKVLGGAV FEGN+ERW+T+ K VVKNISKDDK+F+ SI+KLSVD
Sbjct: 365 REEVVTKIGGIPLVAKVLGGAVKFEGNYERWMTKFKSVVKNISKDDKDFISSILKLSVDC 424
Query: 423 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 482
LPLLS KQCFAYCSNFPKD+ F KESLIQ WIAQGF+QPQE D+E+M+ED GE YF+ LL
Sbjct: 425 LPLLSLKQCFAYCSNFPKDYKFCKESLIQTWIAQGFIQPQEEDNELMMEDIGEGYFDLLL 484
Query: 483 SRSLFEVVIKG---LITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGT 542
SRSLFEVV + T F MHDLIHDIAC ISN +VES+L+NS EKSV KLRT+I
Sbjct: 485 SRSLFEVVDEDSNERTTHFKMHDLIHDIACAISN--QVESNLHNSSEKSVRKLRTIIHDI 544
Query: 543 GYKVF--HEDLKHHACLRVLVVDTII-YDKNFSGPIGMF-KHLRYLDISDISGDFELKDV 602
+ EDL CLRVLV +D + IG+ KHLRYL+I + + +
Sbjct: 545 DNSICDKFEDL---VCLRVLVFALHPGWDVSLFNSIGIIAKHLRYLEIKQCYHELKY-SL 604
Query: 603 ESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYE 662
ESI LYNLQTLKLG LP NLRKLVKLRHL+F I KMPLHM QLIHLQTL+A+
Sbjct: 605 ESIAMLYNLQTLKLGVGASLPTNLRKLVKLRHLEFKDIGDGKMPLHMRQLIHLQTLTAFF 664
Query: 663 ASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKE 722
E G KIEELGPLKNLKGSL L +LG+VKSKEEA+GARLIEKKNL L WNSSS+
Sbjct: 665 VG-EDGCKIEELGPLKNLKGSLDLGSLGEVKSKEEAMGARLIEKKNLTKLAFYWNSSSE- 724
Query: 723 KGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKL 782
EEDN N EVL+GLQPH+NLQSLSI Y+GQ FPN+IFVE L
Sbjct: 725 -----------EEDNL-------NELEVLEGLQPHNNLQSLSILHYNGQAFPNNIFVEGL 784
Query: 783 VKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKI 842
V I L C KCEMLPMLGQLPNLEEL IS +DSV+SIGREFYG +++NQRV FPKLKK
Sbjct: 785 VHIMLHGCMKCEMLPMLGQLPNLEELVISEVDSVKSIGREFYG-SNNSNQRVCFPKLKKF 844
Query: 843 SLHFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPN 902
SL +++NLE WED+ NG+AF HL I+ + C KL S+PH F
Sbjct: 845 SLMYMENLENWEDVPNGMAFSHLTILYMRGCPKL-SIPHHF------------------- 904
Query: 903 QLEHYNSIKSLKISDCPNLSLNVCN-WSNLEEMEITGCMQDYDFSPFTHLCSITDLSLTD 962
E NSIKSL CP L N+CN ++ LE+MEI GCM+DYDFSP H SIT +SLTD
Sbjct: 905 --ECLNSIKSLTHIHCPKLPQNMCNQYNKLEDMEIWGCMKDYDFSPLIHQSSITRVSLTD 964
Query: 963 SLENN-ATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFASLEALHLSDCKNLKRLPS 1022
+ E+N A ELPQQL +LTTLK L I F GIEALP+WL N A LE L L CKNLK LPS
Sbjct: 965 TSEDNPAIELPQQLGYLTTLKLLEIFGFEGIEALPDWLENLACLEELKLYYCKNLKWLPS 1008
Query: 1023 KEVMLCLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLP 1050
K VML LTKLN++ V GCPL L L +G PE AKLSH P
Sbjct: 1025 KHVMLSLTKLNRVEV-KGCPL-LPLGEGDPERAKLSHFP 1008
BLAST of Spg005214 vs. NCBI nr
Match:
XP_022145177.1 (putative disease resistance protein RGA3 [Momordica charantia])
HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 630/1057 (59.60%), Postives = 744/1057 (70.39%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAVQEVL+K+VK+AA+QIG+AWGLEKELSKL+ LLKAE L DI+R++LHH+
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+W+++LQ V+EA DLLDELVYEDLR+KVE K KVR S S N F FR KM K
Sbjct: 61 SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEIGK-MNKVRGSISRSYNLFSFRRKMAK 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNSEVIGREAEVSN 180
K+K+VTETL + Y E S G LVG ES ETN+ +LKQIRETTS LN EV+GRE EVSN
Sbjct: 121 KMKDVTETLYKHYCEASPLG---LVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSN 180
Query: 181 IVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITN 240
IVKL IDSSNE+ SIIPIVGMGG+GKTTLA+MIFNHE+IKGHFD+TIW+CVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIIN 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKES 300
ILE IF LT TS+G SKE L++ LQE+M GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 ILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQI 300
Query: 301 AGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIR 360
KSGN IIVTTRS++VA +ET SHH+ KLSDDQ W LFK SA ANGL MNP+L IR
Sbjct: 301 TEKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDIIR 360
Query: 361 VELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRL 420
LVKKI GVPL+AKVLGGA+ FEG++E WV +VK VV+NISKDDK+FVLSI+KLSVD L
Sbjct: 361 EILVKKIGGVPLVAKVLGGAIKFEGDYETWVKKVKSVVRNISKDDKDFVLSILKLSVDCL 420
Query: 421 PLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLS 480
PLLS KQCFAYCSNF KD +FDKE+LIQMWIAQGF+Q EG +E ED GE + NFLLS
Sbjct: 421 PLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNFLLS 480
Query: 481 RSLFEVVIKGL---ITRFTMHDLIHDIACDISNHQK-VESSLNNSGEKSVSKLRTLICGT 540
RSLF+ V+K + +T F MHDLIHDIAC ISNH K +E +N KS KLRTLIC
Sbjct: 481 RSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLICNN 540
Query: 541 GYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDV-ESI 600
+V + +L LRVLV+++ D N IG KHLRYLDIS + ++ + ESI
Sbjct: 541 --QVIYNELMDCVSLRVLVLNSAKTD-NLLDSIGKLKHLRYLDIS----HYSIRVLPESI 600
Query: 601 DKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSR-IKTKKMPLHMSQLIHLQTLSAYEAS 660
KLYNLQTLK+G LPKNLRK+V LRHL+F T +MP HMS LIHLQTL + A
Sbjct: 601 AKLYNLQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAG 660
Query: 661 YEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKG 720
+E G KIEELGPLKNLKG L+L NL +VKSKEEAI A+L+EKKNL +L W
Sbjct: 661 FENGCKIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDW-------- 720
Query: 721 NHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVK 780
S E N+DD+ EVL+GLQPH NLQSL+I + GQV P +IFVE L
Sbjct: 721 ------SLDFERNDDDS-------EVLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAV 780
Query: 781 IFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISL 840
I L KCEMLPMLGQLPNL EL+IS +DSVR IG EFYG+ SF KL+K
Sbjct: 781 IRLCGGKKCEMLPMLGQLPNLVELEISFMDSVRCIGSEFYGNG-------SFSKLRKFDF 840
Query: 841 HFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPNQL 900
L NLEQW + + S+ + F SLQTL + GC L++LPN L
Sbjct: 841 IGLHNLEQWTEAA--------------------SISNSFGSLQTLNVIGCGNLKKLPNGL 900
Query: 901 EHYNSIKSLKISDCPNLSLNVCNW---------------------SNLEEMEITGCMQDY 960
E +S+ L + DCP L+LNV N +NL+EME+ GCMQDY
Sbjct: 901 EFCSSLHDLTVRDCPKLALNVQNMHNLSNLFIHGFKELPLGLSHLTNLKEMEVVGCMQDY 960
Query: 961 DFSPFTHLCSITDLSLTDSLENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFAS 1020
DFSPF L S+ L L D + TELPQ ++LT L+TL+I F+ IEALPEWL N AS
Sbjct: 961 DFSPFLSLQSLIKLHLND-VSRICTELPQHFENLTALETLTIVNFDAIEALPEWLENLAS 996
Query: 1021 LEALHLSDCKNLKRLPSKEVMLCLTKLNKLNVGYGCP 1031
LE L+L DCKNLKRLPS+E ML LTKL L V + CP
Sbjct: 1021 LETLNLEDCKNLKRLPSREAMLRLTKLEHLKV-FRCP 996
BLAST of Spg005214 vs. NCBI nr
Match:
XP_038895415.1 (disease resistance protein RGA2-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1069.3 bits (2764), Expect = 2.3e-308
Identity = 625/1057 (59.13%), Postives = 722/1057 (68.31%), Query Frame = 0
Query: 3 EFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESV 62
EFLWTF VQE LKK+VK+AA++I VAWG +KEL KL+ LLK+EA LRDI ++L++E V
Sbjct: 5 EFLWTFVVQEALKKVVKVAAQEIAVAWGFKKELEKLTKWLLKSEAILRDIITKKLYNEPV 64
Query: 63 RMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKL 122
RMWV++LQH VYEADDLLDELVYEDLR+K ETEK ++KV FFS S N FLFR KM K++
Sbjct: 65 RMWVEDLQHFVYEADDLLDELVYEDLRRKAETEKIEKKVHNFFSCSHNPFLFRRKMAKRM 124
Query: 123 KEVTETLCQLYHETSSFGVVALVGQESTETNKNI-LKQIRETTSTLNSE--VIGREAEVS 182
K++TETL + Y E S G+VALVG ESTE KNI LKQIR+TTS L E +IGRE EVS
Sbjct: 125 KDITETLYKHYCEASPLGLVALVGHESTEARKNIDLKQIRDTTSKLKPEEVIIGREVEVS 184
Query: 183 NIVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIIT 242
NIVKL I+SSNE+PMSIIPIVGMGG+GKTTLAKM+FNH L++ HFD+T+WVCVSEPFII
Sbjct: 185 NIVKLVIESSNEHPMSIIPIVGMGGLGKTTLAKMVFNHGLVQTHFDKTLWVCVSEPFIII 244
Query: 243 NILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKE 302
NILE IF SLT TS+ +KENLL+ LQE+M+G +YFLVLDDVWNEEA LW LKDCL E
Sbjct: 245 NILEGIFQSLTKTSSSCLNKENLLQRLQEEMRGNNYFLVLDDVWNEEAILWDNLKDCLNE 304
Query: 303 SAGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTI 362
AGK GN IIVTTRS VA +M TT S H+ KLSDD CWSLFK+ A+GL EL+ I
Sbjct: 305 IAGKPGNTIIVTTRSPDVATVMGTTSSIHLKKLSDDLCWSLFKKGVSADGL----ELEII 364
Query: 363 RVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDR 422
R E+V KI G+PL+AKVLGGAV FEGN+ERW+T+ K VVKNISKDDK+F+ SI+KLSVD
Sbjct: 365 REEVVTKIGGIPLVAKVLGGAVKFEGNYERWMTKFKSVVKNISKDDKDFISSILKLSVDC 424
Query: 423 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 482
LPLLS KQCFAYCSNFPKD+ F KESLIQ WIAQGF+QPQE D+E+M+ED GE YF+ LL
Sbjct: 425 LPLLSLKQCFAYCSNFPKDYKFCKESLIQTWIAQGFIQPQEEDNELMMEDIGEGYFDLLL 484
Query: 483 SRSLFEVVIKG---LITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGT 542
SRSLFEVV + T F MHDLIHDIAC ISN +VES+L+NS EKSV KLRT+I
Sbjct: 485 SRSLFEVVDEDSNERTTHFKMHDLIHDIACAISN--QVESNLHNSSEKSVRKLRTIIHDI 544
Query: 543 GYKVF--HEDLKHHACLRVLVVDTII-YDKNFSGPIGMF-KHLRYLDISDISGDFELKDV 602
+ EDL CLRVLV +D + IG+ KHLRYL+I + + +
Sbjct: 545 DNSICDKFEDL---VCLRVLVFALHPGWDVSLFNSIGIIAKHLRYLEIKQCYHELKY-SL 604
Query: 603 ESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYE 662
ESI LYNLQTLKLG LP NLRKLVKLRHL+F I KMPLHM QLIHLQTL+A+
Sbjct: 605 ESIAMLYNLQTLKLGVGASLPTNLRKLVKLRHLEFKDIGDGKMPLHMRQLIHLQTLTAFF 664
Query: 663 ASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKE 722
E G KIEELGPLKNLKGSL L +LG+VKSKEEA+GARLIEKKNL L WNSSS+
Sbjct: 665 VG-EDGCKIEELGPLKNLKGSLDLGSLGEVKSKEEAMGARLIEKKNLTKLAFYWNSSSE- 724
Query: 723 KGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKL 782
EEDN N EVL+GLQPH+NLQSLSI Y+GQ FPN+IFVE L
Sbjct: 725 -----------EEDNL-------NELEVLEGLQPHNNLQSLSILHYNGQAFPNNIFVEGL 784
Query: 783 VKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKI 842
V I L C KCEMLPMLGQLPNLEEL IS +DSV+SIGREFYG +++NQRV FPKLKK
Sbjct: 785 VHIMLHGCMKCEMLPMLGQLPNLEELVISEVDSVKSIGREFYG-SNNSNQRVCFPKLKKF 844
Query: 843 SLHFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPN 902
SL +++NLE WED+ N
Sbjct: 845 SLMYMENLENWEDVPN-------------------------------------------- 904
Query: 903 QLEHYNSIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSITDLSLTDS 962
S
Sbjct: 905 ---------------------------------------------------------DTS 927
Query: 963 LENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFASLEALHLSDCKNLKRLPSKE 1022
+N A ELPQQL +LTTLK L I F GIEALP+WL N A LE L L CKNLK LPSK
Sbjct: 965 EDNPAIELPQQLGYLTTLKLLEIFGFEGIEALPDWLENLACLEELKLYYCKNLKWLPSKH 927
Query: 1023 VMLCLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLP 1050
VML LTKLN++ V GCPL L L +G PE AKLSH P
Sbjct: 1025 VMLSLTKLNRVEV-KGCPL-LPLGEGDPERAKLSHFP 927
BLAST of Spg005214 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 428.3 bits (1100), Expect = 2.7e-118
Identity = 352/1067 (32.99%), Postives = 521/1067 (48.83%), Query Frame = 0
Query: 10 VQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNL 69
+Q +L L ++G+ +G EKE KLS++ +A L D ++L +++++ W+ L
Sbjct: 6 LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+LD+ K E + K+ V + P + F YK+ K++KE+ E L
Sbjct: 66 NVAAYEVDDILDDC-------KTEAARFKQAVLGRYHP--RTITFCYKVGKRMKEMMEKL 125
Query: 130 CQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNS-EVIGREAEVSNIVKLAIDS 189
+ E +F + + + R+T L +V GRE E IVK+ I++
Sbjct: 126 DAIAEERRNFHLDERIIERQAAR--------RQTGFVLTEPKVYGREKEEDEIVKILINN 185
Query: 190 -SNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFH 249
S + ++PI+GMGG+GKTTLA+M+FN + I HF+ IWVCVS+ F +++ I
Sbjct: 186 VSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVE 245
Query: 250 SLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGNV 309
S+ S G L K LQE + GK YFLVLDDVWNE+ W L+ LK G SG
Sbjct: 246 SIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGAS 305
Query: 310 IIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIRVELVKKI 369
I++TTR K+ +IM T + ++ LS + CW LFK+ A + +P+L I E+VKK
Sbjct: 306 ILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKC 365
Query: 370 DGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFKQ 429
GVPL AK LGG + F+ W + N+ +D+ N VL ++LS LP L +Q
Sbjct: 366 GGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDE-NSVLPALRLSYHHLP-LDLRQ 425
Query: 430 CFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLSRSLF-EV 489
CFAYC+ FPKD +KE LI +W+A F+ + M +ED G E +N L RS F E+
Sbjct: 426 CFAYCAVFPKDTKIEKEYLIALWMAHSFLLSK---GNMELEDVGNEVWNELYLRSFFQEI 485
Query: 490 VIKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGTGYK-----VF 549
+K T F MHDLIHD+A + + S+ K + ++ T YK F
Sbjct: 486 EVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIV--TNYKDMMSIGF 545
Query: 550 HEDL--------KHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDVE 609
E + K LRVL + +++ +G HLRYL D+SG+ +
Sbjct: 546 SEVVSSYSPSLFKRFVSLRVLNLSNSEFEQ-LPSSVGDLVHLRYL---DLSGNKICSLPK 605
Query: 610 SIDKLYNLQTLKL---GERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSA 669
+ KL NLQTL L LPK KL LR+L MP + L L+TL
Sbjct: 606 RLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTLGY 665
Query: 670 YEASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSS 729
+ +G+++ EL L NL+G++ + +L +VK+ EA A L K NL L ++W+
Sbjct: 666 FVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDR-- 725
Query: 730 KEKGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFP---NSI 789
N + +VL+ L+PH NL+ L I + G P N
Sbjct: 726 -------------------PNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHS 785
Query: 790 FVEKLVKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFP 849
++ +V I + C C LP G+LP LE L++ S+ E+ D +R FP
Sbjct: 786 VLKNVVSILISGCENCSCLPPFGELPCLESLELQD----GSVEVEYVEDSGFLTRR-RFP 845
Query: 850 KLKKISLHFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKEL 909
L+K+ + NL+ + + FP LE + I C M V S++ L + G +
Sbjct: 846 SLRKLHIGGFCNLKGLQRMKGAEQFPVLEEMKISDC--PMFVFPTLSSVKKLEIWGEADA 905
Query: 910 RELPNQLEHYNSIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSITDL 969
L + + + +++ SLKI N S LEEM F +L ++ L
Sbjct: 906 GGL-SSISNLSTLTSLKIFS------NHTVTSLLEEM-------------FKNLENLIYL 965
Query: 970 SLTDSLENNATELPQQLQHLTTLKTLSIRRFNGIEALP-EWLGNFASLEALHLSDCKNLK 1029
S+ S N ELP L L LK L IR +E+LP E L +SL L + C LK
Sbjct: 966 SV--SFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLK 988
Query: 1030 RLPSKEVMLCLTKLNKLNVGYGCP-LGLQLRQGHPE-MAKLSHLPKI 1052
LP E + LT L L + GCP L + +G E K+SH+P +
Sbjct: 1026 CLP--EGLQHLTTLTSLKI-RGCPQLIKRCEKGIGEDWHKISHIPNV 988
BLAST of Spg005214 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 407.5 bits (1046), Expect = 5.0e-112
Identity = 342/1041 (32.85%), Postives = 504/1041 (48.41%), Query Frame = 0
Query: 10 VQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNL 69
+Q +L+ L +++ + +G EKE KLS++ +A L+D ++L +++ W+ L
Sbjct: 6 LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+L E K E + ++ F+ P FR+K+ +++KE+ E L
Sbjct: 66 NSAAYEVDDILGEC-------KNEAIRFEQSRLGFYHP--GIINFRHKIGRRMKEIMEKL 125
Query: 130 CQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNS-EVIGREAEVSNIVKLAIDS 189
+ E F + + T + RET L +V GR+ E IVK+ I++
Sbjct: 126 DAISEERRKFHFLEKI------TERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINN 185
Query: 190 SN-ENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFH 249
N + + PI+GMGG+GKTTLA+MIFN E + HF+ IWVCVS+ F +++ I
Sbjct: 186 VNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIG 245
Query: 250 SLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGNV 309
++ +S + + K LQE + GK Y LVLDDVWN++ W +L+ L + G G
Sbjct: 246 NIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGAS 305
Query: 310 IIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIRVELVKKI 369
I+ TTR KV +IM T +H++ LS LF + A NP L I E+VKK
Sbjct: 306 ILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKC 365
Query: 370 DGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFKQ 429
GVPL AK LGG + F+ W + ++ +D+ + +L ++LS LP L +Q
Sbjct: 366 GGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESS-ILPALRLSYHHLP-LDLRQ 425
Query: 430 CFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLSRSLF-EV 489
CFAYC+ FPKD KE+LI +W+A GF+ + + +ED G E +N L RS F E+
Sbjct: 426 CFAYCAVFPKDTKMIKENLITLWMAHGFLLSK---GNLELEDVGNEVWNELYLRSFFQEI 485
Query: 490 VIKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLR-TLICGTGYKVFHED- 549
K T F +HDLIHD+A + + +S N E +V + T+ G V
Sbjct: 486 EAKSGNTYFKIHDLIHDLATSLFS---ASASCGNIREINVKDYKHTVSIGFAAVVSSYSP 545
Query: 550 --LKHHACLRVLVVDTIIYDK--NFSGPIGMFKHLRYLDISDISGDFELKDVESIDKLYN 609
LK LRVL + Y K IG HLRYLD+S +F E + KL N
Sbjct: 546 SLLKKFVSLRVL---NLSYSKLEQLPSSIGDLLHLRYLDLS--CNNFRSLP-ERLCKLQN 605
Query: 610 LQTLKLGERIP---LPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYEASYEQ 669
LQTL + LPK KL LRHL P + L L+TL + ++
Sbjct: 606 LQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKK 665
Query: 670 GHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKGNHW 729
G+++ EL L NL GS+ + +L +VK+ +A A L K NL L ++W
Sbjct: 666 GYQLGELKNL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSW----------- 725
Query: 730 NNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFP---NSIFVEKLVK 789
DN+ N + +VL+ L+PH NL+ L I + G FP N +EK++
Sbjct: 726 --------DNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVIS 785
Query: 790 IFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRV----SFPKLK 849
+ +K C C LP G+LP LE L++ + Y ++DD + R SFP LK
Sbjct: 786 VRIKSCKNCLCLPPFGELPCLENLELQN-----GSAEVEYVEEDDVHSRFSTRRSFPSLK 845
Query: 850 KISLHFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELREL 909
K+ + F ++L+ FP LE ++I C L P S++ L + G R L
Sbjct: 846 KLRIWFFRSLKGLMKEEGEEKFPMLEEMAI-LYCPLFVFP-TLSSVKKLEVHGNTNTRGL 905
Query: 910 PNQLEHYNSIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSITDLSLT 969
+ + + +++ SL+I N S EEM FT L ++ LS
Sbjct: 906 -SSISNLSTLTSLRI------GANYRATSLPEEM-------------FTSLTNLEFLSFF 962
Query: 970 DSLENNATELPQQLQHLTTLKTLSIRRFNGIEALPE-WLGNFASLEALHLSDCKNLKRLP 1029
D N +LP L L LK L I + +E+ PE L SL L + CK LK LP
Sbjct: 966 DF--KNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP 962
Query: 1030 SKEVMLCLTKLNKLNVGYGCP 1031
E + LT L L V GCP
Sbjct: 1026 --EGLQHLTALTNLGVS-GCP 962
BLAST of Spg005214 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 406.0 bits (1042), Expect = 1.4e-111
Identity = 327/1054 (31.02%), Postives = 500/1054 (47.44%), Query Frame = 0
Query: 10 VQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNL 69
+Q VL L ++ + +G + E +LS++ +A L D ++L+ + + W+ L
Sbjct: 6 IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+LDE +T+ T+ ++ FR+K+ K++ +V + L
Sbjct: 66 NAATYEVDDILDE---------YKTKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVMKKL 125
Query: 130 CQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNS-EVIGREAEVSNIVKLAID- 189
+ E F + + + T RET S L +V GR+ E IVK+ I+
Sbjct: 126 NAIAEERKKFHLQEKIIERQAAT--------RETGSVLTEPQVYGRDKEKDEIVKILINT 185
Query: 190 SSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFH 249
+S+ +S++PI+GMGG+GKTTL++M+FN + + F IW+C+S+ F +++ I
Sbjct: 186 ASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVE 245
Query: 250 SLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGNV 309
S+ S L K LQE + GK YFLVLDDVWNE+ W L+ LK G SG
Sbjct: 246 SIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAF 305
Query: 310 IIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIRVELVKKI 369
++ TTR KV +IM T + ++ LS + CW LF + A + +NP L I E+VKK
Sbjct: 306 VLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKC 365
Query: 370 DGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFKQ 429
GVPL AK LGG + F+ W + N+ +D+ + +L ++LS LP L +Q
Sbjct: 366 GGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESS-ILPALRLSYHHLP-LDLRQ 425
Query: 430 CFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLSRSLF-EV 489
CF YC+ FPKD KE+LI W+A GF+ + + +ED G E +N L RS F E+
Sbjct: 426 CFVYCAVFPKDTKMAKENLIAFWMAHGFLLSK---GNLELEDVGNEVWNELYLRSFFQEI 485
Query: 490 VIKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGTGYKVFHED-L 549
++ T F MHDLIHD+A + + S++ + ++ + L
Sbjct: 486 EVESGKTYFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYSPSLL 545
Query: 550 KHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDV-ESIDKLYNLQTL 609
+ LRVL + ++ IG HLRYL D+SG+F ++++ + + KL NLQTL
Sbjct: 546 QKFVSLRVLNLRNSNLNQ-LPSSIGDLVHLRYL---DLSGNFRIRNLPKRLCKLQNLQTL 605
Query: 610 KL---GERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYEASYEQGHKI 669
L LPK KL LR+L P + L L++LS + +GH++
Sbjct: 606 DLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQL 665
Query: 670 EELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKGNHWNNSS 729
EL L NL GS+ + L +VK +A A L K NL L L+W+ K
Sbjct: 666 GELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWDLDGK---------- 725
Query: 730 SGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFP---NSIFVEKLVKIFLK 789
H EVL+ L+PH NL+ L IN + G P N ++ +V I ++
Sbjct: 726 ------------HRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIR 785
Query: 790 DCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISLHFLQ 849
C C LP G+LP LE L++ + G +DN FP L+K+ +
Sbjct: 786 GCENCSCLPPFGELPCLESLELHT-------GSADVEYVEDNVHPGRFPSLRKLVIWDFS 845
Query: 850 NLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPNQLEHYN 909
NL+ + FP LE ++ C + +P L K L+ +
Sbjct: 846 NLKGLLKMEGEKQFPVLEEMTF-YWCPMFVIP---------TLSSVKTLKVIVTDATVLR 905
Query: 910 SIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSITDLSLTDSLENNAT 969
SI +L+ ++S NV S EEM F L ++ L + S N
Sbjct: 906 SISNLRALTSLDISDNVEATSLPEEM-------------FKSLANLKYLKI--SFFRNLK 965
Query: 970 ELPQQLQHLTTLKTLSIRRFNGIEALP-EWLGNFASLEALHLSDCKNLKRLPSKEVMLCL 1029
ELP L L LK+L + +E+LP E + SL L +S+C LK LP E + L
Sbjct: 966 ELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLP--EGLQHL 973
Query: 1030 TKLNKLNVGYGCPLGLQL--RQGHPEMAKLSHLP 1050
T L L + CP+ + R + K++H+P
Sbjct: 1026 TALTTLTITQ-CPIVFKRCERGIGEDWHKIAHIP 973
BLAST of Spg005214 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 403.3 bits (1035), Expect = 9.4e-111
Identity = 321/1009 (31.81%), Postives = 493/1009 (48.86%), Query Frame = 0
Query: 10 VQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNL 69
+Q +L L ++ + +G + E +LS++ +A L D ++L+++ + W+ L
Sbjct: 6 IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65
Query: 70 QHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETL 129
A YE DD+LDE +T+ T+ ++ FR+K+ K++ +V + L
Sbjct: 66 NAATYEVDDILDE---------YKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKL 125
Query: 130 CQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNS-EVIGREAEVSNIVKLAIDS 189
+ E +F + + + RET S L +V GR+ E IVK+ I++
Sbjct: 126 KAIAEERKNFHLHEKIVERQAVR--------RETGSVLTEPQVYGRDKEKDEIVKILINN 185
Query: 190 -SNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFH 249
S+ +S++PI+GMGG+GKTTLA+M+FN + + HF IW+CVSE F +++ I
Sbjct: 186 VSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVE 245
Query: 250 SLTNTS-NGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGN 309
S+ G L K LQE + GK Y LVLDDVWNE+ W L+ LK G SG
Sbjct: 246 SIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGA 305
Query: 310 VIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIRVELVKK 369
++ TTR KV +IM T + ++ LS + CW LF + A + +NP L I E+VKK
Sbjct: 306 SVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKK 365
Query: 370 IDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFK 429
GVPL AK LGG + F+ W + N+ +D+ + +L ++LS +LP L K
Sbjct: 366 SGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESS-ILPALRLSYHQLP-LDLK 425
Query: 430 QCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLSRSLF-E 489
QCFAYC+ FPKD +KE LI +W+A GF+ + M +ED G+E + L RS F E
Sbjct: 426 QCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSK---GNMELEDVGDEVWKELYLRSFFQE 485
Query: 490 VVIKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGTGYKVFH--- 549
+ +K T F MHDLIHD+A + + S++ + S + + ++ G VF
Sbjct: 486 IEVKDGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSI--GFAEVVFFYTL 545
Query: 550 EDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDVESIDKLYNLQ 609
L+ LRVL + ++K IG HLRYL++ SG L + + KL NLQ
Sbjct: 546 PPLEKFISLRVLNLGDSTFNK-LPSSIGDLVHLRYLNLYG-SGMRSLP--KQLCKLQNLQ 605
Query: 610 TLKL---GERIPLPKNLRKLVKLRHLQFSRIKTKK-MPLHMSQLIHLQTLSAYEASYEQG 669
TL L + LPK KL LR+L ++ MP + L L+TL + ++G
Sbjct: 606 TLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKG 665
Query: 670 HKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKGNHWN 729
+++ ELG L NL GS+K+ +L +VK+ ++A A L K NL L ++WN
Sbjct: 666 YQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWN----------- 725
Query: 730 NSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFP---NSIFVEKLVKI 789
N + + +VL+ L+PH NL SL I + G P N ++ +V I
Sbjct: 726 --------NFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSI 785
Query: 790 FLKDCGKCEMLPMLGQLPNLEELDI----SSLDSVRSIGREFYGDKDDNNQRVSFPKLKK 849
+ + C LP G LP LE L++ + ++ V + + + R+ FP L+K
Sbjct: 786 LISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFP---TRIRFPSLRK 845
Query: 850 ISLHFLQNLEQWEDISNGIAFPHLEIVSIEKC---------CKLMSVPHCF--------- 909
+ + +L+ FP LE + I +C L S+ C+
Sbjct: 846 LDIWDFGSLKGLLKKEGEEQFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFPE 905
Query: 910 ------VSLQTLVLEGCKELRELPNQLEHYNSIKSLKISDCPNLSLNVCNWSNLEEMEIT 969
+L+ L + C L+ELP L N++KSLKI C C +L E +
Sbjct: 906 EMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLC-------CALESLPEEGLE 940
Query: 970 GCMQDYDFSPFTHLCSITDLSLTDSLENNATELPQQLQHLTTLKTLSIR 977
G L S+T+L + N LP+ LQHLTTL +L IR
Sbjct: 966 G------------LSSLTELFVEHC--NMLKCLPEGLQHLTTLTSLKIR 940
BLAST of Spg005214 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 373.6 bits (958), Expect = 8.0e-102
Identity = 307/999 (30.73%), Postives = 500/999 (50.05%), Query Frame = 0
Query: 32 EKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNLQHAVYEADDLLDELVYEDLRQK 91
E L +LS LL A L D + +++ + V WV+ L+ VY A+D LD++ E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 92 VETEKTK----EKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLYHETSSFGVVALVGQ 151
+ E + ++R S D + +L++VT L +L + + G+ L
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155
Query: 152 ESTETNKNILKQIRETTSTLN-SEVIGREAEVSNIVKLAI-DSSNENPMSIIPIVGMGGV 211
I KQ TTS ++ SEV GR+ + I++ I ++ +N ++++ IVG+GGV
Sbjct: 156 --------IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 215
Query: 212 GKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFHSLTNTSNGFKSKENLLKG 271
GKTTL+++++N + ++ +F +W VSE F + I ++++ S+T+ F + L
Sbjct: 216 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 275
Query: 272 LQEKMQGKS--YFLVLDDVWNEEASLWGELKDCLKESAGKSGNVIIVTTRSVKVANIMET 331
L+E++ G + LVLDD+WNE + W L+ +A G+ I+VTTRS +VA+IM
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCA 335
Query: 332 TLSHHVNKLSDDQCWSLFKESAKAN-GLLMNPELKTIRVELVKKIDGVPLIAKVLGGAVT 391
H++ LSD CWSLF ++ N +N E+ + +V K G+PL K LGG +
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 392 FEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFKQCFAYCSNFPKDHLFD 451
FEG W + + ++ D N +L ++++S LP K+CFAYCS FPK H F+
Sbjct: 396 FEGKVIEWERVLSSRIWDLPADKSN-LLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 455
Query: 452 KESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLSRSLFEVVIKGLITRFTMHDLIH 511
K+ ++ +W+A+GF+Q +E+ G EYF+ L SRSL ++ TR+ MHD I+
Sbjct: 456 KDKVVLLWMAEGFLQQTRSSKN--LEELGNEYFSELESRSL----LQKTKTRYIMHDFIN 515
Query: 512 DIA----------------CDISNHQKVESSLNNS--------GEKSVSKLRTLI----- 571
++A +S + S L ++ + V LRT +
Sbjct: 516 ELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLT 575
Query: 572 -----CGTGYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFE 631
C V + L LRVL ++ + K P FK++ + D+S
Sbjct: 576 NSSRSCCLDQMVSEKLLPTLTRLRVL---SLSHYKIARLPPDFFKNISHARFLDLSRTEL 635
Query: 632 LKDVESIDKLYNLQTLKL---GERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHL 691
K +S+ +YNLQTL L LP ++ L+ LR+L K ++MP +L L
Sbjct: 636 EKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSL 695
Query: 692 QTLSAYEASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLT 751
QTL+ + S G +I ELG L +L G LK++ L +V +A A L KK+L ++
Sbjct: 696 QTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFV 755
Query: 752 WNSSSKEKGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPN 811
W + SSS E + N N EV + L+PH +++ L+I Y G+ FP+
Sbjct: 756 WRT----------GSSSSENNTNPHRT--QNEAEVFEKLRPHRHIEKLAIERYKGRRFPD 815
Query: 812 SI---FVEKLVKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFY-GDKDDNN 871
+ ++V I L++C C LP LGQLP L+EL IS + ++SIGR+FY D+ +
Sbjct: 816 WLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRD 875
Query: 872 Q-RVSFPKLKKISLHFLQNLEQWED--ISNGIAFPHLEIVSIEKCCKLM-SVPHCFVSLQ 931
Q + F L+ + L + ++W D ++ G FP L+ + I +C +L ++P SL
Sbjct: 876 QDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLI 935
Query: 932 TLVLEGCKELRELPNQLEH-YNSIKSLKI-SDCPNL-SLNVCNWSNLEEMEITGCMQDYD 974
+L + C L P+ E+ Y ++++L I S C L + +++NL+++E+ C Y
Sbjct: 936 SLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKFPLNHFANLDKLEVDQCTSLYS 995
BLAST of Spg005214 vs. ExPASy TrEMBL
Match:
A0A6J1CUL8 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111014734 PE=4 SV=1)
HSP 1 Score: 1222.2 bits (3161), Expect = 0.0e+00
Identity = 670/1059 (63.27%), Postives = 797/1059 (75.26%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAVQEVLKK++KLAAEQIGVAW L+KELSKL LLKAE L DI+R++L HE
Sbjct: 1 MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+WV++LQH VY+ADDLLD+LVYEDLRQKVE EK KVR F SPS+N FLFR+KM K
Sbjct: 61 SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKI-NKVRNFLSPSNNPFLFRFKMAK 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETN-KNILKQIRETTSTLNSEVIGREAEVS 180
++K ++ETL + Y E S V+ VG+ESTET KNIL+QIRETTS LN +VIGRE EV
Sbjct: 121 RMKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVL 180
Query: 181 NIVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIIT 240
I+KL IDSS+E+ MSIIPIVGMGG+GKTTLAKM+FNHELIK HFD+TIWVCVS+PFII
Sbjct: 181 KIMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIV 240
Query: 241 NILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKE 300
NILE IF SLTNTS+G KE L LQ++MQGK YFLVLDDVWNEE +LW EL+DCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300
Query: 301 SAGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTI 360
A KSGN IIVTTRS VA IM+T SH + KLSDD+CWSLFKESA ANGLLMNPEL+ I
Sbjct: 301 IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360
Query: 361 RVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDR 420
R +VK+I G+PL+AKVLGG FEGN+ERWVT V+ +V+NI D+K+FVLS +KLSVD
Sbjct: 361 REVVVKRIGGIPLVAKVLGGTAKFEGNYERWVTTVESIVRNILNDEKDFVLSTLKLSVDS 420
Query: 421 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 480
LPLLS KQCFA+CSNFPKD F KE+LIQMWIAQGF+Q +EGD+ ++ E+ GE YFNFLL
Sbjct: 421 LPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEGDNVVLEENIGEGYFNFLL 480
Query: 481 SRSLFEVV---IKGLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGT 540
SRSLF+ V I G IT F MHDLIHD+AC +SN QK+E N EK V LRTL+
Sbjct: 481 SRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRALRTLVINN 540
Query: 541 GYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDVESID 600
G + H L+ LRVLV+++ + KN IGM KHLRYL DI G + ESI
Sbjct: 541 G-AIIHNKLEDSVYLRVLVLNS-DFIKNLPDSIGMLKHLRYL---DIIGSRIRRLPESIT 600
Query: 601 KLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYEASYE 660
LYNLQ LKL RI LPKNLRKLV LRHL F I +KMPLH+SQLIHLQTL Y +E
Sbjct: 601 MLYNLQILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYIVGFE 660
Query: 661 QGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKGNH 720
+G KIEELGPL +LKGSL+LLNL +VKSKEEAI A+LIEK NL +L L H
Sbjct: 661 KGRKIEELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTL-----------H 720
Query: 721 WNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIF 780
WN+SS GEE N + N EVL+GLQPH NLQSLSI + G PN +FVE LV I
Sbjct: 721 WNSSSCGEEGYN-----NRNDSEVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAIV 780
Query: 781 LKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISLHF 840
L++C KCEMLPMLGQLPNLEEL +S + SVR IG EFYG+ ++ +++ FPKL+++S
Sbjct: 781 LQECNKCEMLPMLGQLPNLEELRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFSH 840
Query: 841 LQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVP-HCFVSLQTLVLEGCKELRELPNQLE 900
++ LEQWE++SNGI FPHLE +S+ C KL SVP H FVSL+TL + GC+EL +LP++LE
Sbjct: 841 MEKLEQWEELSNGINFPHLETLSMFYCSKLTSVPDHEFVSLRTLRIVGCEELSKLPSKLE 900
Query: 901 HYNSIKSLKISDCPNLSLNVCNWSNLEEMEITGCMQDYDFSPFTHLCSITDLSLTDSLEN 960
N +KIS CP L LN+ N NL E+EI G M++YDFS H S+T++ LTDSL
Sbjct: 901 CCN---FMKISGCPKLRLNMHNMCNLNELEINGPMEEYDFSFLMHQSSVTNIFLTDSL-R 960
Query: 961 NATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFASLEALHLSDCKNLKRLPSKEVML 1020
NAT+LPQQLQHLTTLK+L+I FNG+EALPEWL A+LE + + +CKNLKRLPS++ ML
Sbjct: 961 NATQLPQQLQHLTTLKSLAIEYFNGVEALPEWLEKLANLETMGVFECKNLKRLPSQQAML 1020
Query: 1021 CLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLPKICDD 1055
LTKL L V GCPL L L QG E AKLSHLP D+
Sbjct: 1021 RLTKLKHLRV-RGCPL-LPLGQGDLERAKLSHLPITVDN 1027
BLAST of Spg005214 vs. ExPASy TrEMBL
Match:
A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)
HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 630/1057 (59.60%), Postives = 744/1057 (70.39%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAVQEVL+K+VK+AA+QIG+AWGLEKELSKL+ LLKAE L DI+R++LHH+
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+W+++LQ V+EA DLLDELVYEDLR+KVE K KVR S S N F FR KM K
Sbjct: 61 SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEIGK-MNKVRGSISRSYNLFSFRRKMAK 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNSEVIGREAEVSN 180
K+K+VTETL + Y E S G LVG ES ETN+ +LKQIRETTS LN EV+GRE EVSN
Sbjct: 121 KMKDVTETLYKHYCEASPLG---LVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSN 180
Query: 181 IVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITN 240
IVKL IDSSNE+ SIIPIVGMGG+GKTTLA+MIFNHE+IKGHFD+TIW+CVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIIN 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKES 300
ILE IF LT TS+G SKE L++ LQE+M GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 ILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQI 300
Query: 301 AGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIR 360
KSGN IIVTTRS++VA +ET SHH+ KLSDDQ W LFK SA ANGL MNP+L IR
Sbjct: 301 TEKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDIIR 360
Query: 361 VELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRL 420
LVKKI GVPL+AKVLGGA+ FEG++E WV +VK VV+NISKDDK+FVLSI+KLSVD L
Sbjct: 361 EILVKKIGGVPLVAKVLGGAIKFEGDYETWVKKVKSVVRNISKDDKDFVLSILKLSVDCL 420
Query: 421 PLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLS 480
PLLS KQCFAYCSNF KD +FDKE+LIQMWIAQGF+Q EG +E ED GE + NFLLS
Sbjct: 421 PLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETTEEDIGEWHLNFLLS 480
Query: 481 RSLFEVVIKGL---ITRFTMHDLIHDIACDISNHQK-VESSLNNSGEKSVSKLRTLICGT 540
RSLF+ V+K + +T F MHDLIHDIAC ISNH K +E +N KS KLRTLIC
Sbjct: 481 RSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKLRTLICNN 540
Query: 541 GYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDV-ESI 600
+V + +L LRVLV+++ D N IG KHLRYLDIS + ++ + ESI
Sbjct: 541 --QVIYNELMDCVSLRVLVLNSAKTD-NLLDSIGKLKHLRYLDIS----HYSIRVLPESI 600
Query: 601 DKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSR-IKTKKMPLHMSQLIHLQTLSAYEAS 660
KLYNLQTLK+G LPKNLRK+V LRHL+F T +MP HMS LIHLQTL + A
Sbjct: 601 AKLYNLQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFAAG 660
Query: 661 YEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKG 720
+E G KIEELGPLKNLKG L+L NL +VKSKEEAI A+L+EKKNL +L W
Sbjct: 661 FENGCKIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDW-------- 720
Query: 721 NHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVK 780
S E N+DD+ EVL+GLQPH NLQSL+I + GQV P +IFVE L
Sbjct: 721 ------SLDFERNDDDS-------EVLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAV 780
Query: 781 IFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISL 840
I L KCEMLPMLGQLPNL EL+IS +DSVR IG EFYG+ SF KL+K
Sbjct: 781 IRLCGGKKCEMLPMLGQLPNLVELEISFMDSVRCIGSEFYGNG-------SFSKLRKFDF 840
Query: 841 HFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPNQL 900
L NLEQW + + S+ + F SLQTL + GC L++LPN L
Sbjct: 841 IGLHNLEQWTEAA--------------------SISNSFGSLQTLNVIGCGNLKKLPNGL 900
Query: 901 EHYNSIKSLKISDCPNLSLNVCNW---------------------SNLEEMEITGCMQDY 960
E +S+ L + DCP L+LNV N +NL+EME+ GCMQDY
Sbjct: 901 EFCSSLHDLTVRDCPKLALNVQNMHNLSNLFIHGFKELPLGLSHLTNLKEMEVVGCMQDY 960
Query: 961 DFSPFTHLCSITDLSLTDSLENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGNFAS 1020
DFSPF L S+ L L D + TELPQ ++LT L+TL+I F+ IEALPEWL N AS
Sbjct: 961 DFSPFLSLQSLIKLHLND-VSRICTELPQHFENLTALETLTIVNFDAIEALPEWLENLAS 996
Query: 1021 LEALHLSDCKNLKRLPSKEVMLCLTKLNKLNVGYGCP 1031
LE L+L DCKNLKRLPS+E ML LTKL L V + CP
Sbjct: 1021 LETLNLEDCKNLKRLPSREAMLRLTKLEHLKV-FRCP 996
BLAST of Spg005214 vs. ExPASy TrEMBL
Match:
A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)
HSP 1 Score: 1062.0 bits (2745), Expect = 1.8e-306
Identity = 608/1061 (57.30%), Postives = 735/1061 (69.27%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAVQEVL+K+VK+AA+QIG+A GLEKELS L+ L KAE LRDI+R++ H +
Sbjct: 1 MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSHSD 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SVR+WV++LQ V+EADDLLDELVYE LR+KVE K KVR FS S N FLFR KM K
Sbjct: 61 SVRLWVEDLQDVVHEADDLLDELVYEHLRRKVEIGKI-NKVRDSFSTSHNVFLFRLKMAK 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNSEVIGREAEVSN 180
K+K++T+ L + Y E S G LVGQESTE N+ +L QIR+TTS LN EV+GRE EVSN
Sbjct: 121 KIKDITQILHKHYCEASPLG---LVGQESTEANQIVLSQIRDTTSNLNFEVVGREDEVSN 180
Query: 181 IVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITN 240
IVKL IDSSNE+ MSI+PIVGMGG+GKTT A+ IFNH+ IKGHFD IWVCVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIIIN 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKES 300
ILE IF SLT TS+G K LL+ +Q++M GK YFLVLDDVWNEE+ LW +L DCLK+
Sbjct: 241 ILEGIFQSLTKTSSGLNHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQI 300
Query: 301 AGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIR 360
AGKSGN IIVTTRS++VANI+ T SH + KLSDDQCW LFKESA AN L MNP+L+ IR
Sbjct: 301 AGKSGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKESANANRLSMNPKLEIIR 360
Query: 361 VELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRL 420
LVKKI GVPL+AKVLGGA FEG++ERWVT+V+ VV+NIS DDK+FV SI+KLSVD L
Sbjct: 361 DMLVKKIGGVPLVAKVLGGATKFEGDYERWVTKVESVVRNISNDDKDFVSSILKLSVDCL 420
Query: 421 PLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQP--QEGDDEMMIEDRGEEYFNFL 480
PLLS KQCFAYCSNF KD L+DKE+LI++WIAQGF+QP QEGD+EM ED GE +FNFL
Sbjct: 421 PLLSLKQCFAYCSNFAKDCLYDKENLIRIWIAQGFIQPQDQEGDNEMTEEDIGEWHFNFL 480
Query: 481 LSRSLFEVVIKGLITR---FTMHDLIHDIACDISNHQKV--ESSLNNSGEKSVSKLRTLI 540
LSRSLF+ V R F MHDLIHDIAC ISNHQK+ E S +N KS KLRTLI
Sbjct: 481 LSRSLFQDVTMDKSERGIYFIMHDLIHDIACAISNHQKLLHELSSSNRSRKSARKLRTLI 540
Query: 541 CGTGYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDVE 600
C +V + +L LRVLV+ N IG KHLRYL I+ +G++ E
Sbjct: 541 CNN--QVIYNELVDCVSLRVLVLH-CEKTGNLLESIGKLKHLRYLSIA--NGNYVDVLPE 600
Query: 601 SIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFS-RIKTKKMPLHMSQLIHLQTLSAYE 660
SI LYNLQTLKL LPKN RK+V LRHL+F + ++MP HMS+LIHLQTLS +
Sbjct: 601 SIVMLYNLQTLKLRHMKSLPKNSRKMVSLRHLEFELKSDFRQMPSHMSELIHLQTLSRFV 660
Query: 661 ASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKE 720
A +E G KIEELGPLKNLKG L+L L +V+SKEE+I A+L+EKKNL +L+ W+S +
Sbjct: 661 AGFENGCKIEELGPLKNLKGKLQLSCLERVESKEESIVAKLVEKKNLQELIFEWSSRFEG 720
Query: 721 KGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKL 780
K + + + EVL+GLQPH NLQSL++ + GQV ++IFVE L
Sbjct: 721 KNSRY------------------SELEVLEGLQPHKNLQSLTVIGFGGQVLGDAIFVENL 780
Query: 781 VKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKI 840
I L +C CE LPMLG+LPNL EL+I+S+DS+R IG EFYG+ NNQ SF KL+K+
Sbjct: 781 TVIRLSNCKNCERLPMLGRLPNLVELNINSMDSLRCIGSEFYGN-GSNNQGHSFSKLRKL 840
Query: 841 SLHFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPN 900
+ + +NLEQW+D + V + F SLQTL + GC L +LPN
Sbjct: 841 TFIWCRNLEQWKDAA--------------------VVSNSFGSLQTLSIYGCGNLEKLPN 900
Query: 901 QLEHYNSIKSLKISDCPNLSLNVCN---------------------WSNLEEMEITGCMQ 960
LE +S++ L ++ CP LSLNV N +NL+EM+ GCMQ
Sbjct: 901 GLEGCSSLRDLTVAYCPKLSLNVQNCQNLSNLRIDGLKKLPLGLGHLTNLKEMKFVGCMQ 960
Query: 961 DYDFSPFTHLCS-ITDLSLTDSLENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGN 1020
DYDF+PF L S I L L D N T+LP LQHLT LK LSI F GIE PEWL N
Sbjct: 961 DYDFTPFVSLQSLIIKLELRDECSGNCTQLPPHLQHLTALKQLSIGNFGGIEVPPEWLNN 1012
Query: 1021 FASLEALHLSDCKNLKRLPSKEVMLCLTKLNKLNVGYGCPL 1032
SLE L C+NL+RLPS E + LTKL L+ CPL
Sbjct: 1021 LTSLEMLSFEGCENLRRLPSTEAIRRLTKLELLS-AIKCPL 1012
BLAST of Spg005214 vs. ExPASy TrEMBL
Match:
A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)
HSP 1 Score: 1003.4 bits (2593), Expect = 7.6e-289
Identity = 574/1088 (52.76%), Postives = 732/1088 (67.28%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAVQEVLKK+V AEQI +AWGLEKELS L LLKA+ L DI+ ++ HH
Sbjct: 1 MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SV +WV+ L +YEADDLLDE+VYE +RQ VE KVR SPS NSFLF KM K
Sbjct: 61 SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMAK 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETNKNILKQIRETTSTLNSEVIGREAEVSN 180
K+K++T+TL + Y E S G LVG EST ++ L QIRETTS L+ EV GREAEV
Sbjct: 121 KMKKITKTLYEHYCEASPLG---LVGDESTTESEAALNQIRETTSILDFEVEGREAEVLE 180
Query: 181 IVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITN 240
I+KL IDS++E+ +S+I IVGMGG+GKTTLAKM+FNH+ IKGHFD+T+WVCVS+PFI+
Sbjct: 181 ILKLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMK 240
Query: 241 ILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKES 300
ILE IF LTNTS+G S+E LL L+E+MQGK YFLVLDDVW++E LW EL LK
Sbjct: 241 ILEAIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYI 300
Query: 301 AGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPELKTIR 360
AGKSGN I+VTTRSV+VA +++T +H+ KLSDD CW+L K+SA AN L MN +L+ +
Sbjct: 301 AGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENTK 360
Query: 361 VELVKKIDGVPLIAKVLGGAVTF-EGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDR 420
LV+KI GVPLIAKVLGGAV F EG E W+ +++ +NIS +DK+FVLSI+KLSV+
Sbjct: 361 NILVRKIGGVPLIAKVLGGAVKFEEGGSESWMAKIESFARNISIEDKDFVLSILKLSVES 420
Query: 421 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 480
LP + KQCFAYCSNFP+D+ FDK+ IQMWIA+GF+QP++ + + +E+ GEEY NFLL
Sbjct: 421 LPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERENLTMENIGEEYLNFLL 480
Query: 481 SRSLFEVVIK--GLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGTG 540
SRSLFE IK G I F +HDL+HDIAC ISNH K++S+ + KS KLRTLIC
Sbjct: 481 SRSLFEDAIKYDGRIVTFKIHDLMHDIACAISNHHKMDSNPISWNGKSTRKLRTLIC-EN 540
Query: 541 YKVFHEDLKHHACLRVLVVDTIIYDKN-FSGPIGMFKHLRYLDISDISGDFELKDVESID 600
+ FH+ CLRVLV+ +D N S + HLRYLDIS+ + + L+D SI
Sbjct: 541 EEAFHKIQTDIICLRVLVLKW--FDTNTLSTIMDKLIHLRYLDISNCNINKLLRD--SIC 600
Query: 601 KLYNLQTLKLGE-RIPLPKNLRKLVKLRHLQFSR-IKTKKMPLHMSQLIHLQTLSAYEAS 660
LYNLQTLKLG LPKNLR LV LRHL+F + +MP HM +IHLQTLS +
Sbjct: 601 ALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFVVG 660
Query: 661 YEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSSKEKG 720
E+G KI+ELGPLK+LKG+L L NL V++K+EA+ A+L+EKK L L+ W + ++G
Sbjct: 661 LEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYDRG 720
Query: 721 NHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVK 780
+ +ED+N +VL+GLQPH N+QSL I + G+V N+IFVE LV+
Sbjct: 721 EY-------DEDDNK---------QVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVE 780
Query: 781 IFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISL 840
I L DCG+CE+LPMLGQLPNL++L+I S++SVRSIG EFYG ++ +FP+L K +
Sbjct: 781 IRLVDCGRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHI 840
Query: 841 HFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPNQL 900
L+ L+QW++ + + + F L+ L+L GC +L +LP+ L
Sbjct: 841 CGLKKLQQWDEAT-------------------VFASNRFGCLKELILSGCHQLAKLPSGL 900
Query: 901 EHYNSIKSLKISDCPNLSLNVCN---------------------WSNLEEMEITGCMQDY 960
E SI+ L I CPNL LNV N +NL+++ I GCMQ+Y
Sbjct: 901 EGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLPDEFGKLTNLKKLRIGGCMQNY 960
Query: 961 DFSPFTHLCS-ITDLSLTD--SLENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLGN 1020
+FSPF HL S + +L LTD S + T+LPQQLQHLT LK L I F+ IE LPEWLGN
Sbjct: 961 EFSPFIHLSSQLVELELTDDGSSGSETTQLPQQLQHLTNLKVLKIADFDDIEVLPEWLGN 1020
Query: 1021 FASLEALHLSDCKNLKRLPSKEVMLCLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLPK 1059
L L +CKNLK LPS+E + LTKL+ L + GCP L L +G E AKLSHLP
Sbjct: 1021 LTCLATLVFLECKNLKELPSREAIQRLTKLDDLVID-GCP-KLLLGEGDQERAKLSHLPS 1043
BLAST of Spg005214 vs. ExPASy TrEMBL
Match:
A0A1S3BXM2 (disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103494527 PE=4 SV=1)
HSP 1 Score: 949.9 bits (2454), Expect = 9.9e-273
Identity = 569/1080 (52.69%), Postives = 701/1080 (64.91%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAEFLWTFAV+E LK+ VK+AA++I V WGLE ELS LS LL A A LRDIDR+ L E
Sbjct: 1 MAEFLWTFAVEETLKRTVKVAAQKIAVVWGLEDELSNLSKWLLDAGALLRDIDREILRKE 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
SV+ WVD L+ V EA+DLLDEL YEDLR+KVET T F S + L R+ M
Sbjct: 61 SVKRWVDGLEDIVSEAEDLLDELAYEDLRRKVET--TSRVCNNFKFSSVLNPLVRHDMAC 120
Query: 121 KLKEVTETLCQLYHETSSFGVVALVGQESTETNK--NILKQIRETTSTLNSEVIGREAEV 180
K+K +T+ L Q Y +++ G LVG+ES E N L+QIRETTS LN +V+GREAEV
Sbjct: 121 KVKTITKMLKQHYRKSAPLG---LVGKESMEKEDGGNNLRQIRETTSILNFDVVGREAEV 180
Query: 181 SNIVKLAIDSS---NENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEP 240
+I++L IDSS +E P+ I+PIVGMGGVGKTTLAK++F HELIK HF ETIWVCVSE
Sbjct: 181 LDILRLVIDSSSNESELPLLIVPIVGMGGVGKTTLAKLVFRHELIKKHFHETIWVCVSEN 240
Query: 241 FIITNILEEIFHSLTNTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKD 300
F I IL I SL++ +E LL+ LQ+++Q K FLVLDDVWNE + LW EL+D
Sbjct: 241 FNIDEILVAILESLSDKVP--TRREALLRRLQKELQDKRCFLVLDDVWNESSKLWEELED 300
Query: 301 CLKESAGKSGNVIIVTTRSVKVANIMETTLSHHVNKLSDDQCWSLFKESAKANGLLMNPE 360
CLKE GK G I+VTTR +VANIM T + + KL +D CWSLFK SA ANG+ M P+
Sbjct: 301 CLKEIVGKFGITIMVTTRLDEVANIMGTVSGYRLEKLPEDHCWSLFKRSANANGIKMTPK 360
Query: 361 LKTIRVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNISKDDKNFVLSIVKL 420
L+ IR++L++KIDG+PL+AKVLGGAV FEG+ +RW T ++ VV+ I K++VLSI++L
Sbjct: 361 LEAIRIKLLQKIDGIPLVAKVLGGAVEFEGDLDRWETTLESVVREIPMKQKSYVLSILQL 420
Query: 421 SVDRLPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYF 480
SVDRLP + KQCFAYCS FPKD KESLI+MWIAQGF+QP EG++ MM+ED GE YF
Sbjct: 421 SVDRLPFVE-KQCFAYCSIFPKDCEVVKESLIRMWIAQGFIQPPEGEN-MMMEDLGEGYF 480
Query: 481 NFLLSRSLFEVVIK---GLITRFTMHDLIHDIACDI-SNHQKVESSLNNSGEKSVSKLRT 540
NFLLSRSLF+ V+K G IT F MHDLIHD+A I SN QK+ + K+ KLRT
Sbjct: 481 NFLLSRSLFQDVVKDKYGSITHFKMHDLIHDVALAILSNRQKMVLDPTHWNGKTARKLRT 540
Query: 541 LICGTGYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKD 600
LI + H L LRVL V+++ N I KHLRYLDIS
Sbjct: 541 LIYNN--QEIHHKLADCVFLRVLEVNSLRMMNNLPDFIAKLKHLRYLDISSCP---MWAI 600
Query: 601 VESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFS-RIKTKKMPLHMSQLIHLQTLSA 660
SI L+NLQTLKLG LP NLR LV+LRHL+F T+KMP HM +LIHLQTLS
Sbjct: 601 PHSITTLFNLQTLKLGSIENLPMNLRNLVRLRHLEFHVYYNTRKMPPHMGELIHLQTLSG 660
Query: 661 YEASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNSSS 720
+ A +E+G KIEELG LKNL+G L+L NL +V+SK+EAI +L++KKNL +L W+
Sbjct: 661 FVAGFEEGCKIEELGNLKNLRGKLQLSNLEQVRSKQEAIAVKLVDKKNLRELTFEWSIDL 720
Query: 721 KEKGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVE 780
+ + +N+ FEVL+GLQP NL SL I + G+ P FVE
Sbjct: 721 LREFSSYND------------------FEVLEGLQPPKNLTSLKITNFGGKFLPAGTFVE 780
Query: 781 KLVKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLK 840
L + L C KCE LPMLGQLPNL+EL I LD V SIG EFYG +D+N+R FPKLK
Sbjct: 781 NLAFLCLYGCTKCERLPMLGQLPNLQELGICFLDRVISIGNEFYG--NDSNRRGYFPKLK 840
Query: 841 KISLHFLQNLEQWEDISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELREL 900
K L ++ NLEQWE LE+ + E + F SLQTL L+ C +L +L
Sbjct: 841 KFDLGWMCNLEQWE----------LEVANHES--------NHFGSLQTLKLDRCGKLTKL 900
Query: 901 PNQLEHYNSIKSLKISDCPNLSLNV---------------------CNWSNLEEMEITGC 960
PN LE S++ + IS+CPNL+LNV + NL+ M ITGC
Sbjct: 901 PNGLECCKSVREVIISNCPNLTLNVEKMHSLSVLLIDGLKFLPKGLAHLPNLKTMMITGC 960
Query: 961 MQDYDFSPFTHLCSITDLSLTDSLENNATELPQQLQHLTTLKTLSIRRFNGIEALPEWLG 1020
++DYD+SPF +L S+T L L D L NAT+LPQQLQHLT LK L+I F GIE LPEWL
Sbjct: 961 IEDYDYSPFLNLPSLTKLYLNDGL-GNATQLPQQLQHLTALKILAIENFYGIEVLPEWLR 1020
Query: 1021 NFASLEALHLSDCKNLKRLPSKEVMLCLTKLNKLNVGYGCPLGLQLRQGHPEMAKLSHLP 1050
LE L L CKNLKRLPS+E MLCLTKL V CPL L Q E AK H+P
Sbjct: 1021 KLTCLETLDLVRCKNLKRLPSREAMLCLTKLKDFKV-MACPLLLLGGQADQEGAKYLHIP 1026
BLAST of Spg005214 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 373.6 bits (958), Expect = 5.7e-103
Identity = 307/999 (30.73%), Postives = 500/999 (50.05%), Query Frame = 0
Query: 32 EKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNLQHAVYEADDLLDELVYEDLRQK 91
E L +LS LL A L D + +++ + V WV+ L+ VY A+D LD++ E LR
Sbjct: 36 ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95
Query: 92 VETEKTK----EKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLYHETSSFGVVALVGQ 151
+ E + ++R S D + +L++VT L +L + + G+ L
Sbjct: 96 IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155
Query: 152 ESTETNKNILKQIRETTSTLN-SEVIGREAEVSNIVKLAI-DSSNENPMSIIPIVGMGGV 211
I KQ TTS ++ SEV GR+ + I++ I ++ +N ++++ IVG+GGV
Sbjct: 156 --------IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGV 215
Query: 212 GKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFHSLTNTSNGFKSKENLLKG 271
GKTTL+++++N + ++ +F +W VSE F + I ++++ S+T+ F + L
Sbjct: 216 GKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVK 275
Query: 272 LQEKMQGKS--YFLVLDDVWNEEASLWGELKDCLKESAGKSGNVIIVTTRSVKVANIMET 331
L+E++ G + LVLDD+WNE + W L+ +A G+ I+VTTRS +VA+IM
Sbjct: 276 LKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMCA 335
Query: 332 TLSHHVNKLSDDQCWSLFKESAKAN-GLLMNPELKTIRVELVKKIDGVPLIAKVLGGAVT 391
H++ LSD CWSLF ++ N +N E+ + +V K G+PL K LGG +
Sbjct: 336 VHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLR 395
Query: 392 FEGNFERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFKQCFAYCSNFPKDHLFD 451
FEG W + + ++ D N +L ++++S LP K+CFAYCS FPK H F+
Sbjct: 396 FEGKVIEWERVLSSRIWDLPADKSN-LLPVLRVSYYYLP-AHLKRCFAYCSIFPKGHAFE 455
Query: 452 KESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLLSRSLFEVVIKGLITRFTMHDLIH 511
K+ ++ +W+A+GF+Q +E+ G EYF+ L SRSL ++ TR+ MHD I+
Sbjct: 456 KDKVVLLWMAEGFLQQTRSSKN--LEELGNEYFSELESRSL----LQKTKTRYIMHDFIN 515
Query: 512 DIA----------------CDISNHQKVESSLNNS--------GEKSVSKLRTLI----- 571
++A +S + S L ++ + V LRT +
Sbjct: 516 ELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLT 575
Query: 572 -----CGTGYKVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFE 631
C V + L LRVL ++ + K P FK++ + D+S
Sbjct: 576 NSSRSCCLDQMVSEKLLPTLTRLRVL---SLSHYKIARLPPDFFKNISHARFLDLSRTEL 635
Query: 632 LKDVESIDKLYNLQTLKL---GERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHL 691
K +S+ +YNLQTL L LP ++ L+ LR+L K ++MP +L L
Sbjct: 636 EKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSL 695
Query: 692 QTLSAYEASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLT 751
QTL+ + S G +I ELG L +L G LK++ L +V +A A L KK+L ++
Sbjct: 696 QTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFV 755
Query: 752 WNSSSKEKGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPN 811
W + SSS E + N N EV + L+PH +++ L+I Y G+ FP+
Sbjct: 756 WRT----------GSSSSENNTNPHRT--QNEAEVFEKLRPHRHIEKLAIERYKGRRFPD 815
Query: 812 SI---FVEKLVKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFY-GDKDDNN 871
+ ++V I L++C C LP LGQLP L+EL IS + ++SIGR+FY D+ +
Sbjct: 816 WLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRD 875
Query: 872 Q-RVSFPKLKKISLHFLQNLEQWED--ISNGIAFPHLEIVSIEKCCKLM-SVPHCFVSLQ 931
Q + F L+ + L + ++W D ++ G FP L+ + I +C +L ++P SL
Sbjct: 876 QDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLI 935
Query: 932 TLVLEGCKELRELPNQLEH-YNSIKSLKI-SDCPNL-SLNVCNWSNLEEMEITGCMQDYD 974
+L + C L P+ E+ Y ++++L I S C L + +++NL+++E+ C Y
Sbjct: 936 SLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKFPLNHFANLDKLEVDQCTSLYS 995
BLAST of Spg005214 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 281.2 bits (718), Expect = 3.8e-75
Identity = 278/1042 (26.68%), Postives = 476/1042 (45.68%), Query Frame = 0
Query: 35 LSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNLQHAVYEADDLLDELVYEDLRQKVET 94
L +L L+ A L D D++ H V+ W+ ++ A ++A+D+LDEL E LR++V
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95
Query: 95 EKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLY-HETSSFGVVALVGQESTETN 154
E N R + KK++ E + +L H V+ L +E +ET
Sbjct: 96 E-----AGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEHHVKHIEVIGL--KEYSETR 155
Query: 155 KNILKQIRET--TSTLNSEVIGREAEVSNIVKLAI--DSSNENPMSIIPIVGMGGVGKTT 214
+ +Q + ++GR + +V L + D + ++I +VGM GVGKTT
Sbjct: 156 EPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTT 215
Query: 215 LAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFHSLTNTSNGFKSKENLLKGLQEK 274
L +++FN + HF+ +W+ F + + + + +T+++ + +L L++
Sbjct: 216 LTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKT 275
Query: 275 MQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGNVIIVTTRSVKVANIMETTLSHHV 334
+ GK + LVLDD W+E S W + ++ + G+ I++TTRS V+ + + + +
Sbjct: 276 LSGKRFLLVLDDFWSESDSEWESFQVAFTDA--EEGSKIVLTTRSEIVSTVAKAEKIYQM 335
Query: 335 NKLSDDQCWSLFKESAKANGLL--MNPELKTIRVELVKKIDGVPLIAKVLGGAVTFEGNF 394
+++++CW L A N + +N EL+ I + ++ G+PL A+ + + + N
Sbjct: 336 KLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNP 395
Query: 395 ERWVTEVKRVVKNISKDDKNFVLSIVKLSVDRLPLLSFKQCFAYCSNFPKDHLFDKESLI 454
+ W V KN S N +L ++KLS D LP K+CFA CS FPK H+FD+E L+
Sbjct: 396 DDWYA----VSKNFS-SYTNSILPVLKLSYDSLP-PQLKRCFALCSIFPKGHVFDREELV 455
Query: 455 QMWIAQGFV-QPQEGDDEMMIEDRGEEYFNFLLSRSLFEVVIKGLITRFTMHDLIHDIAC 514
+W+A + QP+ +ED G +Y L+++S F+ + +T F MHDL++D+A
Sbjct: 456 LLWMAIDLLYQPR---SSRRLEDIGNDYLGDLVAQSFFQ-RLDITMTSFVMHDLMNDLAK 515
Query: 515 DIS----------NHQKVESSLN----NSGEKSVSKLRTLICGTGY-------------- 574
+S N ++ S+ + + S ICG +
Sbjct: 516 AVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLE 575
Query: 575 ------KVFHEDLKHHACLRVLVVDTIIYDKNFSGPIGMFKHLRYLDISDISGDFELKDV 634
KV + L + LR+L + + N + K LRYLD+S ++K++
Sbjct: 576 SLQLTEKVLNPLLNALSGLRILSL-SHYQITNLPKSLKGLKLLRYLDLSST----KIKEL 635
Query: 635 -ESIDKLYNLQTLKLG---ERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTL 694
E + L NLQTL L + LPK++ +L+ LR L +MP + +L LQ L
Sbjct: 636 PEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKL 695
Query: 695 SAYEASYEQGHKIEELGPLKNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVLTWNS 754
S + G + EL L +L+G+L++ L V EA A L K L L+L W
Sbjct: 696 SNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWT- 755
Query: 755 SSKEKGNHWNNSSSGEEDNNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFP---- 814
KG+ + S N + EVL+ L+PH +L++ I Y G FP
Sbjct: 756 ---VKGSGFVPGSF--------NALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLG 815
Query: 815 -NSIFVEKLVKIFLKDCGKCEMLPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQR 874
+S F + + L C C LP +GQLP+L+ L I + ++ +G +F+ ++N++
Sbjct: 816 DSSFF--GITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFG-ENNSRG 875
Query: 875 VSFPKLKKISLHFLQNLEQW--EDISNGIAFPHLEIVSIEKCCKL-MSVPHCFVSLQTLV 934
V F L+ + + + ++W ++ +GI FP L+ + I++C L P S +
Sbjct: 876 VPFQSLQILKFYGMPRWDEWICPELEDGI-FPCLQKLIIQRCPSLRKKFPEGLPSSTEVT 935
Query: 935 LEGC----------KELRELPNQLEHYNSIKSLKISDCPNLSLN-VCNWSNLEEMEITGC 994
+ C R L N E SI S+ + + + N + S +
Sbjct: 936 ISDCPLRAVSGGENSFRRSLTNIPESPASIPSMSRRELSSPTGNPKSDASTSAQPGFASS 995
Query: 995 MQDYDFSPFTHLCSITDLSLT------DSLENNATELPQQLQHLTTLKTLSIRRFNG-IE 1005
Q D + T S++ L D E LPQQ + + R++G I
Sbjct: 996 SQSNDDNEVTSTSSLSSLPKDRQTEDFDQYETQLGSLPQQFEEPAVISA----RYSGYIS 1032
BLAST of Spg005214 vs. TAIR 10
Match:
AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 218.4 bits (555), Expect = 3.0e-56
Identity = 237/960 (24.69%), Postives = 441/960 (45.94%), Query Frame = 0
Query: 1 MAEFLWTFAVQEVLKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHE 60
MAE + +F V+ KL +L + + G+++++ L L + ++ L+D D ++ E
Sbjct: 1 MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60
Query: 61 SVRMWVDNLQHAVYEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTK 120
VR ++++++ VY+ADD+++ + +LR K + K + + F + R K
Sbjct: 61 RVRNFLEDVKDIVYDADDIIESFLLNELRGKEKGIKKQVRTLACF------LVDRRKFAS 120
Query: 121 KLKEVTETLCQLYHETSSFGV--VALVGQESTETNKNILKQIRETTS-TLNSEVIGREAE 180
++ +T+ + ++ S G+ +A G S + ++IR+T S S+++G +
Sbjct: 121 DIEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQER-QREIRQTFSRNSESDLVGLDQS 180
Query: 181 VSNIVKLAIDSSNENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFI 240
V +V +++ + + ++ + GMGG+GKTTLA+ +F+H++++ HFD WVCVS+ F
Sbjct: 181 VEELVDHLVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFT 240
Query: 241 ITNILEEIFHSLTNTSNG-FKSKENLLKG-LQEKMQGKSYFLVLDDVWNEEASLWGELKD 300
++ + I L G + E L+G L E ++ Y LVLDDVW EE W +K
Sbjct: 241 RKDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKA 300
Query: 301 CLKESAGKSGNVIIVTTRSVKVA-NIMETTLSHHVNKLSDDQCWSLFKE--SAKANGLLM 360
K G +++T+R+ + + T + L+ +Q W LF+ S++ +
Sbjct: 301 VFPH---KRGWKMLLTSRNEGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSRRDKTEF 360
Query: 361 NPELKTIRVELVKKIDGVPLIAKVLGGAVTFEGNFERWVTEVKRVVKNI------SKDDK 420
+ + + E+V G+PL KVLGG + + W +V +I S D+
Sbjct: 361 KVD-EAMGKEMVTYCGGLPLAVKVLGGLLAKKHTVLEWKRVHSNIVTHIVGKSGLSDDNS 420
Query: 421 NFVLSIVKLSVDRLPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMM 480
N V ++ LS + LP + K CF Y ++FP+D+ D + L W+A+G + P D
Sbjct: 421 NSVYRVLSLSYEDLP-MQLKHCFFYLAHFPEDYKIDVKILFNYWVAEGIITPFH--DGST 480
Query: 481 IEDRGEEYFNFLLSRSLFEVVIKGLITRF---TMHDLIHDIACDISNHQ------KVESS 540
I+D GE Y L+ R++ V L +R MHD++ ++ + + KV ++
Sbjct: 481 IQDTGESYLEELVRRNMVVVEESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVVKVPTT 540
Query: 541 LNNS-GEKSVSKLRTLICGTG---YKVFHEDLKH------------------HACLRVLV 600
+ + +S + R L+ +G + + H+D K CL +L
Sbjct: 541 TSTTINAQSPCRSRRLVLHSGNALHMLGHKDNKKARSVLIFGVEEKFWKPRGFQCLPLLR 600
Query: 601 VDTIIYDKNFSG----PIGMFKHLRYLDISDISGDFELKDVESIDKLYNLQTLKLGER-- 660
V + Y + G IG HLR+L + + +G L S+ L L L LG
Sbjct: 601 VLDLSYVQFEGGKLPSSIGDLIHLRFLSLYE-AGVSHLP--SSLGNLKLLLCLNLGVADR 660
Query: 661 --IPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSAYEASYEQGHKIEELGPL 720
+ +P L+++ +LR+L+ R K L + L++L++L+ + + G +
Sbjct: 661 LLVHVPNVLKEMQELRYLRLPRSMPAKTKLELGDLVNLESLTNFSTKH---------GSV 720
Query: 721 KNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDL-VLTWNSSSKEKGNHWNNSSSGEED 780
+L KL L + S E L+ + L +L L+++ K +
Sbjct: 721 TDLLRMTKLSVLNVIFSGECTFETLLLSLRELRNLETLSFHDFQKVSVAN---------- 780
Query: 781 NNDDNCFHGNSFEVLKGLQPHHNLQSLSINLYSGQVFPNSIFVEKLVKIFLKDCGKCE-M 840
HG VL + +L+ L+++++ + F L I+L C E
Sbjct: 781 -------HGGELLVLDFI----HLKDLTLSMHLPRFPDQYRFPPHLAHIWLIGCRMEEDP 840
Query: 841 LPMLGQLPNLEELDISSLDSVRSIGREFYGDKDDNNQRVSFPKLKKISLHFLQNLEQWED 900
+P+L +L +L+ + +S S +GR K FP+L + + + + L +W
Sbjct: 841 MPILEKLLHLKSVYLS---SGAFLGRRMVCSKG------GFPQLLALKMSYKKELVEWR- 872
Query: 901 ISNGIAFPHLEIVSIEKCCKLMSVPHCFVSLQTLVLEGCKELRELPNQLEHYNSIKSLKI 906
+ G S+P L+TL ++ CK+L++LP+ L++ +K LKI
Sbjct: 901 VEEG------------------SMP----CLRTLTIDNCKKLKQLPDGLKYVTCLKELKI 872
BLAST of Spg005214 vs. TAIR 10
Match:
AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 199.1 bits (505), Expect = 1.9e-50
Identity = 217/803 (27.02%), Postives = 362/803 (45.08%), Query Frame = 0
Query: 14 LKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNLQHAV 73
L+K + + E+ K+L L + L ++ L+D +RQ+ +E++R V +L+ V
Sbjct: 10 LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69
Query: 74 YEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLY 133
YEA+D+L + D E + + + +YK +K+L+E+ E + ++
Sbjct: 70 YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPA---RVPLQYKKSKRLQEINERITKIK 129
Query: 134 HETS---SFGVVALVGQESTETNKNILKQIRETTSTLNSEVIGREAEVSNIVKLAIDSSN 193
+ F + VG+++ + + +++V+G E + I K + SN
Sbjct: 130 SQVEPYFEFITPSNVGRDNGTDRWS-------SPVYDHTQVVGLEGDKRKI-KEWLFRSN 189
Query: 194 ENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFHSLT 253
++ + I+ VGMGG+GKTT+A+ +FN + I+ F+ IWV VS+ F I+ I +L
Sbjct: 190 DSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLG 249
Query: 254 NTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGNVIIV 313
+ S G LL+ +Q+ + GK Y +V+DDVW++ S W ++ L G S +IV
Sbjct: 250 DASVG-DDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGS---VIV 309
Query: 314 TTRSVKVANIMET--TLSHHVNKLSDDQCWSLFKESAKA--NGLLMNPELKTIRVELVKK 373
TTRS VA ++ +H LS D W LF A A +G PEL+ + E+V K
Sbjct: 310 TTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTK 369
Query: 374 IDGVPLIAKVLGGAVTFEGN-FERWVTEVKRVVKNISKD------DKNFVLSIVKLSVDR 433
G+PL K +GG + + + + W +R+ ++ + + + V+S ++LS D
Sbjct: 370 CKGLPLTIKAVGGLLLCKDHVYHEW----RRIAEHFQDELRGNTSETDNVMSSLQLSYDE 429
Query: 434 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 493
LP K C S +P+D + K+ L+ WI +GFV + G + GE+ F+ L
Sbjct: 430 LP-SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNG---RSATESGEDCFSGLT 489
Query: 494 SRSLFEVVIK---GLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGT 553
+R L EVV K G I +HD++ D+ DI+ K +S N G L G
Sbjct: 490 NRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIA---KKDSFSNPEG------LNCRHLGI 549
Query: 554 GYKVFHEDLKHHACLRVLVVDTIIYDKN-----FSGPIGMFKHLRYLDISDISGDFELKD 613
+ +K + LR +V T + N + K+LR LDIS D L +
Sbjct: 550 SGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSE 609
Query: 614 V-ESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSA 673
+ + I L +L L L PL + R + L +LQ L S +L+ L
Sbjct: 610 ILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQI---------LDASYCQNLKQLQP 669
Query: 674 YEASYEQGHKIE--ELGPL----KNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVL 733
+++ ++ G L K + +KL L K G +L E KNL +L
Sbjct: 670 CIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNL-- 729
Query: 734 TWNSSSKEKGNHWNNSSSGEEDNNDD------------NCFHGNSFEV---LKGLQPHHN 773
++ G EE+ D NC+ ++ + L P H
Sbjct: 730 ------RKLGLSLTRGDQIEEEELDSLINLSKLMSISINCYDSYGDDLITKIDALTPPHQ 763
BLAST of Spg005214 vs. TAIR 10
Match:
AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 199.1 bits (505), Expect = 1.9e-50
Identity = 217/803 (27.02%), Postives = 362/803 (45.08%), Query Frame = 0
Query: 14 LKKLVKLAAEQIGVAWGLEKELSKLSNLLLKAEATLRDIDRQRLHHESVRMWVDNLQHAV 73
L+K + + E+ K+L L + L ++ L+D +RQ+ +E++R V +L+ V
Sbjct: 10 LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69
Query: 74 YEADDLLDELVYEDLRQKVETEKTKEKVRKFFSPSDNSFLFRYKMTKKLKEVTETLCQLY 133
YEA+D+L + D E + + + +YK +K+L+E+ E + ++
Sbjct: 70 YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPA---RVPLQYKKSKRLQEINERITKIK 129
Query: 134 HETS---SFGVVALVGQESTETNKNILKQIRETTSTLNSEVIGREAEVSNIVKLAIDSSN 193
+ F + VG+++ + + +++V+G E + I K + SN
Sbjct: 130 SQVEPYFEFITPSNVGRDNGTDRWS-------SPVYDHTQVVGLEGDKRKI-KEWLFRSN 189
Query: 194 ENPMSIIPIVGMGGVGKTTLAKMIFNHELIKGHFDETIWVCVSEPFIITNILEEIFHSLT 253
++ + I+ VGMGG+GKTT+A+ +FN + I+ F+ IWV VS+ F I+ I +L
Sbjct: 190 DSQLLIMAFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLG 249
Query: 254 NTSNGFKSKENLLKGLQEKMQGKSYFLVLDDVWNEEASLWGELKDCLKESAGKSGNVIIV 313
+ S G LL+ +Q+ + GK Y +V+DDVW++ S W ++ L G S +IV
Sbjct: 250 DASVG-DDIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGS---VIV 309
Query: 314 TTRSVKVANIMET--TLSHHVNKLSDDQCWSLFKESAKA--NGLLMNPELKTIRVELVKK 373
TTRS VA ++ +H LS D W LF A A +G PEL+ + E+V K
Sbjct: 310 TTRSESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTK 369
Query: 374 IDGVPLIAKVLGGAVTFEGN-FERWVTEVKRVVKNISKD------DKNFVLSIVKLSVDR 433
G+PL K +GG + + + + W +R+ ++ + + + V+S ++LS D
Sbjct: 370 CKGLPLTIKAVGGLLLCKDHVYHEW----RRIAEHFQDELRGNTSETDNVMSSLQLSYDE 429
Query: 434 LPLLSFKQCFAYCSNFPKDHLFDKESLIQMWIAQGFVQPQEGDDEMMIEDRGEEYFNFLL 493
LP K C S +P+D + K+ L+ WI +GFV + G + GE+ F+ L
Sbjct: 430 LP-SHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNG---RSATESGEDCFSGLT 489
Query: 494 SRSLFEVVIK---GLITRFTMHDLIHDIACDISNHQKVESSLNNSGEKSVSKLRTLICGT 553
+R L EVV K G I +HD++ D+ DI+ K +S N G L G
Sbjct: 490 NRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIA---KKDSFSNPEG------LNCRHLGI 549
Query: 554 GYKVFHEDLKHHACLRVLVVDTIIYDKN-----FSGPIGMFKHLRYLDISDISGDFELKD 613
+ +K + LR +V T + N + K+LR LDIS D L +
Sbjct: 550 SGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSE 609
Query: 614 V-ESIDKLYNLQTLKLGERIPLPKNLRKLVKLRHLQFSRIKTKKMPLHMSQLIHLQTLSA 673
+ + I L +L L L PL + R + L +LQ L S +L+ L
Sbjct: 610 ILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQI---------LDASYCQNLKQLQP 669
Query: 674 YEASYEQGHKIE--ELGPL----KNLKGSLKLLNLGKVKSKEEAIGARLIEKKNLIDLVL 733
+++ ++ G L K + +KL L K G +L E KNL +L
Sbjct: 670 CIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCKLSEVKNLTNL-- 729
Query: 734 TWNSSSKEKGNHWNNSSSGEEDNNDD------------NCFHGNSFEV---LKGLQPHHN 773
++ G EE+ D NC+ ++ + L P H
Sbjct: 730 ------RKLGLSLTRGDQIEEEELDSLINLSKLMSISINCYDSYGDDLITKIDALTPPHQ 763
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038877313.1 | 0.0e+00 | 65.10 | putative disease resistance protein RGA3 [Benincasa hispida] | [more] |
| XP_022145229.1 | 0.0e+00 | 63.27 | putative disease resistance protein RGA4 [Momordica charantia] | [more] |
| XP_038895409.1 | 0.0e+00 | 63.55 | disease resistance protein RGA2-like isoform X1 [Benincasa hispida] | [more] |
| XP_022145177.1 | 0.0e+00 | 59.60 | putative disease resistance protein RGA3 [Momordica charantia] | [more] |
| XP_038895415.1 | 2.3e-308 | 59.13 | disease resistance protein RGA2-like isoform X2 [Benincasa hispida] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q7XA40 | 2.7e-118 | 32.99 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA39 | 5.0e-112 | 32.85 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA42 | 1.4e-111 | 31.02 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XBQ9 | 9.4e-111 | 31.81 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
| Q9LRR4 | 8.0e-102 | 30.73 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CUL8 | 0.0e+00 | 63.27 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A6J1CVR6 | 0.0e+00 | 59.60 | putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A6J1CU86 | 1.8e-306 | 57.30 | disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014... | [more] |
| A0A0A0KST5 | 7.6e-289 | 52.76 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1 | [more] |
| A0A1S3BXM2 | 9.9e-273 | 52.69 | disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103494527 PE=... | [more] |
