Spg005157 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg005157
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description15-cis-zeta-carotene isomerase, chloroplastic
Locationscaffold11: 33959758 .. 33963241 (-)
RNA-Seq ExpressionSpg005157
SyntenySpg005157
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGGCTTATAAGTTGAATGATTTTAAATGCAATTTTTCATCCTAATACATGTATAGTTTATTGGCCAACAGCACAGCTCAACGGACATATAGTTGTATTGATAACCACAAGATTTGTGATTCGAATCCCTCCACTCTACATATTGTCAAAAAAAAAAAAAAAAAAAATTTGTATATCTTACTGCATAAGGACATCTATTCCATCTAGTCAGGAATTCAATTCTCTAAGAAAAGAAATAACAAGAATACATCCTCCAACAAACTTAATTGCCATCCCCTGCGTGACTAGGCCTGCAATTTGCATATACATAATTCCACAAAATCCAAAATCTCCATTCAAGCGCACCAATTGCCTCAGATTCTCCTCCATTTTCCATTACCCCAACTTCCTCAATCCTCATTGCTTTCTCCTCTCACTCTCCACTGACACCCACCTCGGTTTACAGTGCTCCAATGGCGGTAGCCTCCGCCGCGTCCCTCTTTTCGTCCCCATTTCCCTCCTCTTCCAGCCAACCCCACCGTCGCCATCGCCGCCCTGATGCGATTGCCCTCCGACCCTTTTCAATTTCCGCCCCCAATTTCCCTCTGCAACTTCCCTTTTCCCTAAATTCCTCACCTGTTTCTCGACGCCGTCGATTTGCCGCCGAGGCTTCTGTTGGAGACAGCGAGAGTGGTGGTTCGACCCCTGTCTCGGACGACGGCGGCTTCGTCGGCGAGGACGCTGCTGCCTTTGATCTTTCCGAGCAGAAATTGGCGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATCGATAACTCTGCTGGGGGAGTTGGGAAGGCCTTTGTGGATGCTGTTTCTGGACTTTCGGATAGTCATGAGGTTCCCTTCTCTTTCACTTCGTTTCCTTTAATTATTTTTTTCCTTTTTGGAGTATTGGAACATGCTGAAGACTGTATACAAATGATTCCTTTGCCAATCCTGCGGGATTGATTGTTTTCTTCAGGAAAATATCATTATGTTATACTTTCTCCTTTTGGTGTAGAAACAGTGTTCATTGTAATTCTGTTGCTTTATGTATGGTGTACTTCAGAAAGGGAGAGATTATCTTACTCTGAGATGATTTGTAATGGCTGGTTTTGGTTGAAGCCAATGGGTGTTTGTAGCTGGGTGAATTGGCTTCCATTTTCTCTCCTATCCCTTCTGGGAGTTCTAAAAGTTTCTTGCTGTTAAAACTTTAAATGAGTTTTCCCCCCCTAATTTTGGGCCAAGTCATGTGTACTTGATAGATTTTCATTTTTATGATAGGAAAAGATGTCTAACTCTTTAAACTAAAATATGGAATTTTACGAACTCATGTTGCTAGCTGATTTTGTAATTTAGTGACAGTAAGGTTTTGATTTTAAAAACTTGATGTATGAAAGATGAAATTTGATTATGAATGACTGGGGAGGTTGAACTTAGCATATGGTTTATATTGTGCATGACCTTGCTAAATGTCTGAATCTGAGTACATAATCTAAGTCCTGAAACTTTCTTCTGCAGGTTGTGATGTTGGCCCTCATTTTCATTTTTGCCATTGTCCACAGTGGTTTGGCAAGTCTCCGAGATCAGGGCGAGAAGCTTGTCGGAGAACGAGCTTTTCGGGTTCTGTTTGCTGGAGTTTCTCTGCCATTGGCTGTCAGCACTGTGGTAGGTTCCTTCCCTTTTCTTTTCCCTTTAACTTGATGAAGTAAATACGATGTGCTTGAAGTTATCTTAGTTCCTCTTATTCATTCAATTTCTCTGAATGCACACCCATATTGACTTGTTTATACGAAGGAGTTGTATTCAGGTGTATTTCATTAACCATCGATACGACGGAGTACAGTTGTGGCAGCTCCAAGGCATTCCCGGAGTACATCAACTTGTGTGGCTCAGTTCGTTTATCTCCTTCTTCTTCCTCTATCCTTCTACTTTTAATCTGCTAGAGGTCGCAGCAGTTGATAAGCCAAAAATGCACCTTTGGGAAACAGGCATCATTAGAATAACTAGGCATCCACAGGTATGTTTATGTTTCATTATCTTGAATGTTCAGTTAATTTCATTACAGTATCAGAATTTCATTGCTGTTAATTTGGTGGCTTCGGCTTGAATCAGTTGATGAACTAGAGTTGTCATTGCCTCATTGGCATGTTATTCTACTGCATCTAAATTTTCTAATGGGTTAGTCCCTGAACTTTCATACATATATATTTCAAAATTATAGTCTCTAAATTTTCAAACATTGAATTTTAATCCCAAACTTTGCATAGAAAGCTTTTTACTTCTTGTTCTTAACTCTTTAATGAACTATTTAACAAAAAATTGACATAAATACATTTTGTTTATCCCTTGACAAACTTTTTATATCAAGTATATTCGGTTAGGTGTTGAATCTCTCTATACATATTTAGGTTCTTATTAAAGCACTATTTAAGGTCCTGTTTGATAACCATTTGGTTTTTGGTTTTTGAAAATTAAGGCTACAAACGCTACTTCCATCTATAAGTTTCCTTGTGTTGTTATTTATCTTCTTCACATGTTTTCAAAAACCAATTCAATTTTTGAAAACCAAAAAAAAAGTAATTTTCAAAAACTTGTTTTTGTTTTTGAAATTTGACTAGGAATGCAAATGTGTCATTGACAAAGATGAAACCATTCTAAGGAAATTGAGAGAAGACAAGTTTAATTTTCAAAAACCAAAAACCAAGAACCAAATGGTTATCAAACGGGACCTAAAAGATAGCAGTATGAAAGTTTAGAAACCAAAATAGGATTTAAACTGAACTATATTTCACATTGCATGAGTTTTCCCGCAAAAGTCGCATTTCGTTATTTCAGACAAATTTGTGAATAAGAGGGGAGGACAAGAAATACCACCAACCATTAAAGACCTTTTTATGAACACTGACCAAAGCAAATCTTGGTTTCTGCTCGAAGATGGTTGGGCAGGTGATGTGGTGCCTTGCTCACACAGTCTGGATTGGGAACTCTGTTGCAGTAGCAGCTTCCATTGGGTTGATAGGACATCATCTGTTCGGGGTATGGAACGGGGACAGGAGGCTAGCGAAGCGATACGGAGCAGATTTTGAAGCGGTGAAAAGGCGAACGAGCGTCGTCCCATTCGCTGCCATTGTGGATGGTCGTCAAAAGTTGCCCGACGATTACTACAAGGAGTTCATTCGGTTGCCATATATATCGATCACTGCATTGACCATTGGTGCCTACCTTGCTCACCCCCTAATGCAAGCTGCTAGTTACAGGCTTCATTGGTAAGTCTATTTCTATGCAGCCAACTCTCTTGTATGAGAAATATACATCATTGTATAAACCAAGGCATAGTGGATAGAGAAATCTGAGCTAGTCATAGCCTTATTTATTAAATAATTACAAATATAGCAGTTTAAATTGTTGAATGTGAATTTAATTTTATATATTTTGTAAAAAGAAATAAAT

mRNA sequence

ATGGCGGTAGCCTCCGCCGCGTCCCTCTTTTCGTCCCCATTTCCCTCCTCTTCCAGCCAACCCCACCGTCGCCATCGCCGCCCTGATGCGATTGCCCTCCGACCCTTTTCAATTTCCGCCCCCAATTTCCCTCTGCAACTTCCCTTTTCCCTAAATTCCTCACCTGTTTCTCGACGCCGTCGATTTGCCGCCGAGGCTTCTGTTGGAGACAGCGAGAGTGGTGGTTCGACCCCTGTCTCGGACGACGGCGGCTTCGTCGGCGAGGACGCTGCTGCCTTTGATCTTTCCGAGCAGAAATTGGCGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATCGATAACTCTGCTGGGGGAGTTGGGAAGGCCTTTGTGGATGCTGTTTCTGGACTTTCGGATAGTCATGAGGTTGTGATGTTGGCCCTCATTTTCATTTTTGCCATTGTCCACAGTGGTTTGGCAAGTCTCCGAGATCAGGGCGAGAAGCTTGTCGGAGAACGAGCTTTTCGGGTTCTGTTTGCTGGAGTTTCTCTGCCATTGGCTGTCAGCACTGTGGTGTATTTCATTAACCATCGATACGACGGAGTACAGTTGTGGCAGCTCCAAGGCATTCCCGGAGTACATCAACTTGTGTGGCTCAGTTCGTTTATCTCCTTCTTCTTCCTCTATCCTTCTACTTTTAATCTGCTAGAGGTCGCAGCAGTTGATAAGCCAAAAATGCACCTTTGGGAAACAGGCATCATTAGAATAACTAGGCATCCACAGATGGTTGGGCAGGTGATGTGGTGCCTTGCTCACACAGTCTGGATTGGGAACTCTGTTGCAGTAGCAGCTTCCATTGGGTTGATAGGACATCATCTGTTCGGGGTATGGAACGGGGACAGGAGGCTAGCGAAGCGATACGGAGCAGATTTTGAAGCGGTGAAAAGGCGAACGAGCGTCGTCCCATTCGCTGCCATTGTGGATGGTCGTCAAAAGTTGCCCGACGATTACTACAAGGAGTTCATTCGGTTGCCATATATATCGATCACTGCATTGACCATTGGTGCCTACCTTGCTCACCCCCTAATGCAAGCTGCTAGTTACAGGCTTCATTGGTAA

Coding sequence (CDS)

ATGGCGGTAGCCTCCGCCGCGTCCCTCTTTTCGTCCCCATTTCCCTCCTCTTCCAGCCAACCCCACCGTCGCCATCGCCGCCCTGATGCGATTGCCCTCCGACCCTTTTCAATTTCCGCCCCCAATTTCCCTCTGCAACTTCCCTTTTCCCTAAATTCCTCACCTGTTTCTCGACGCCGTCGATTTGCCGCCGAGGCTTCTGTTGGAGACAGCGAGAGTGGTGGTTCGACCCCTGTCTCGGACGACGGCGGCTTCGTCGGCGAGGACGCTGCTGCCTTTGATCTTTCCGAGCAGAAATTGGCGTCGTGGGCTTATTTCACTGTGATTTTGGGGGTTGTTCTGTTCGTGTTGAATGTTGTTTGGATCGATAACTCTGCTGGGGGAGTTGGGAAGGCCTTTGTGGATGCTGTTTCTGGACTTTCGGATAGTCATGAGGTTGTGATGTTGGCCCTCATTTTCATTTTTGCCATTGTCCACAGTGGTTTGGCAAGTCTCCGAGATCAGGGCGAGAAGCTTGTCGGAGAACGAGCTTTTCGGGTTCTGTTTGCTGGAGTTTCTCTGCCATTGGCTGTCAGCACTGTGGTGTATTTCATTAACCATCGATACGACGGAGTACAGTTGTGGCAGCTCCAAGGCATTCCCGGAGTACATCAACTTGTGTGGCTCAGTTCGTTTATCTCCTTCTTCTTCCTCTATCCTTCTACTTTTAATCTGCTAGAGGTCGCAGCAGTTGATAAGCCAAAAATGCACCTTTGGGAAACAGGCATCATTAGAATAACTAGGCATCCACAGATGGTTGGGCAGGTGATGTGGTGCCTTGCTCACACAGTCTGGATTGGGAACTCTGTTGCAGTAGCAGCTTCCATTGGGTTGATAGGACATCATCTGTTCGGGGTATGGAACGGGGACAGGAGGCTAGCGAAGCGATACGGAGCAGATTTTGAAGCGGTGAAAAGGCGAACGAGCGTCGTCCCATTCGCTGCCATTGTGGATGGTCGTCAAAAGTTGCCCGACGATTACTACAAGGAGTTCATTCGGTTGCCATATATATCGATCACTGCATTGACCATTGGTGCCTACCTTGCTCACCCCCTAATGCAAGCTGCTAGTTACAGGCTTCATTGGTAA

Protein sequence

MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRRRFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAYLAHPLMQAASYRLHW
Homology
BLAST of Spg005157 vs. NCBI nr
Match: XP_022972107.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 684.1 bits (1764), Expect = 6.8e-193
Identity = 342/375 (91.20%), Postives = 356/375 (94.93%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA +LF SPFP+SS+QPH RHRRPDA  LRP SIS PNF  QL FSLNSSP+ RRR
Sbjct: 1   MAVASATTLFFSPFPTSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEAS+GDSESGGST  SDD G VGEDAA F+LSEQKLASW YFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQL  IPG+HQLVWLSSFISFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHTVWIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYGADFEAVKRRTSVVPFAAI+DGRQKLPDDYYKEFIRLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 375

BLAST of Spg005157 vs. NCBI nr
Match: XP_022932715.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita moschata] >KAG6597644.1 15-cis-zeta-carotene isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680.2 bits (1754), Expect = 9.8e-192
Identity = 340/375 (90.67%), Postives = 355/375 (94.67%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA +LF  P P+SS+QPH RHRR DA  LRP SIS PNF  QLPFSLNSSP+ RRR
Sbjct: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEAS+GDSESGGST  SDD G VGEDAA F+LSEQKLASWAYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDA+SGLSDSHEVVMLALIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQL  IPG+HQLVWLSSFISFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHTVWIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYGADFEAVKRRTSVVPFAAI+DGRQKLPDDYYKEFIRLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 375

BLAST of Spg005157 vs. NCBI nr
Match: XP_038882546.1 (15-cis-zeta-carotene isomerase, chloroplastic [Benincasa hispida])

HSP 1 Score: 679.1 bits (1751), Expect = 2.2e-191
Identity = 340/375 (90.67%), Postives = 353/375 (94.13%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA   FS PF SS     RRHRRPDA  LRPFSIS PN PLQLPFS NS+P+SRR 
Sbjct: 1   MAVASATPPFSPPFLSS-----RRHRRPDATTLRPFSISTPNCPLQLPFSFNSTPISRRP 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFAAEAS+GDSESGGST VSDDGGFVGEDAAAFDLS+QKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RFAAEASIGDSESGGSTSVSDDGGFVGEDAAAFDLSDQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDAVSG+SDSHEVVML L FIFAIVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGISDSHEVVMLLLTFIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQ +PG+HQLVWLSSF+SFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHT+WIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTIWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYGADFEAVKRRTS+VPFAAIVDGRQKLPDDYYKEF+RLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGADFEAVKRRTSIVPFAAIVDGRQKLPDDYYKEFVRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 370

BLAST of Spg005157 vs. NCBI nr
Match: XP_023540701.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.9 bits (1748), Expect = 4.9e-191
Identity = 341/377 (90.45%), Postives = 356/377 (94.43%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPH--RRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSR 60
           MAVASA +LF  PFPS S+QPH   RHRRPDA+ LRP SIS PN   QLPFSLNSSP+ R
Sbjct: 1   MAVASATTLFFPPFPSPSNQPHHRHRHRRPDALTLRPSSISTPNVLPQLPFSLNSSPIPR 60

Query: 61  RRRFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLN 120
           RRRF+AEAS+GDSESGGST  SDD G VGEDAA F+LS+QKLASWAYFTVILGVVLFVLN
Sbjct: 61  RRRFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSDQKLASWAYFTVILGVVLFVLN 120

Query: 121 VVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAF 180
           VVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFA+VHSGLASLRDQGEKLVGERAF
Sbjct: 121 VVWIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAF 180

Query: 181 RVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNL 240
           RVLFAGVSLPLAVSTVVYFINHRYDGVQLWQL  IPG+HQLVWLSSFISFFFLYPSTFNL
Sbjct: 181 RVLFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNL 240

Query: 241 LEVAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFG 300
           LEVAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHTVWIGNSVAVAASIGLIGHHLFG
Sbjct: 241 LEVAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFG 300

Query: 301 VWNGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIG 360
           VWNGDRRLAKRYGADFEAVKRRTSVVPFAAI+DGRQKLPDDYYKEFIRLPY+SITALTIG
Sbjct: 301 VWNGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIG 360

Query: 361 AYLAHPLMQAASYRLHW 376
           AYLAHPLMQAAS+RLHW
Sbjct: 361 AYLAHPLMQAASFRLHW 377

BLAST of Spg005157 vs. NCBI nr
Match: KAG7029085.1 (15-cis-zeta-carotene isomerase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 664.8 bits (1714), Expect = 4.3e-187
Identity = 340/404 (84.16%), Postives = 355/404 (87.87%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA +LF  P P+SS+QPH RHRR DA  LRP SIS PNF  QLPFSLNSSP+ RRR
Sbjct: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEAS+GDSESGGST  SDD G VGEDAA F+LSEQKLASWAYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDA+SGLSDSHEVVMLALIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTV-----------------------------VYFINHRYDGVQLWQLQ 240
           LFAGVSLPLAVSTV                             VYFINHRYDGVQLWQL 
Sbjct: 181 LFAGVSLPLAVSTVVGSFPLLFPLSSLTAHPHIDLFTLRSCIQVYFINHRYDGVQLWQLH 240

Query: 241 GIPGVHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIIRITRHPQMVGQVMW 300
            IPG+HQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGI+RITRHPQMVGQV+W
Sbjct: 241 SIPGLHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGIMRITRHPQMVGQVIW 300

Query: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAIVD 360
           CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAI+D
Sbjct: 301 CLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAVKRRTSVVPFAAILD 360

Query: 361 GRQKLPDDYYKEFIRLPYISITALTIGAYLAHPLMQAASYRLHW 376
           GRQKLPDDYYKEFIRLPY+SITALTIGAYLAHPLMQAAS+RLHW
Sbjct: 361 GRQKLPDDYYKEFIRLPYLSITALTIGAYLAHPLMQAASFRLHW 404

BLAST of Spg005157 vs. ExPASy Swiss-Prot
Match: Q9SAC0 (15-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=Z-ISO PE=1 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 7.6e-139
Identity = 243/358 (67.88%), Postives = 291/358 (81.28%), Query Frame = 0

Query: 28  PDAIALRPFSISAPNFPL--QLP--------FSLNSSPVSRRRRFAAEASVGDSESGGST 87
           P ++ L P S   PN  L  ++P         SL SS     R+    +++ + +   S 
Sbjct: 11  PPSLLLLPPSPRRPNLTLIRRIPAHPRLGNSTSLLSSSSPVIRKILVRSTLREDQPIASD 70

Query: 88  PVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGGVGKAFVDAV 147
             S     +GED+AAF+L +QKL SW YF V+LGVVLF+LNVVWIDNS  G GK+F+DAV
Sbjct: 71  SESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNST-GFGKSFIDAV 130

Query: 148 SGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVYF 207
           S +S S EV ML LI IFAIVHSGLASLRD GEKL+GERAFRVLFAG+SLPLA+ST+VYF
Sbjct: 131 SNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMSTIVYF 190

Query: 208 INHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGII 267
           INHRYDG QLWQLQG+PGVH+ +W+++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETGI+
Sbjct: 191 INHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIM 250

Query: 268 RITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAV 327
           RITRHPQMVGQ++WCLAHT+WIGN+VA +AS+GLI HHLFG WNGDRRLAKRYG DFE++
Sbjct: 251 RITRHPQMVGQIVWCLAHTLWIGNTVAASASLGLIAHHLFGAWNGDRRLAKRYGEDFESI 310

Query: 328 KRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAYLAHPLMQAASYRLHW 376
           K+RTSV+PFAAI +GRQ LP+DYYKEF+RLPY++ITALT+GAY AHPLMQ AS+RLHW
Sbjct: 311 KKRTSVIPFAAIFEGRQVLPEDYYKEFVRLPYLAITALTVGAYFAHPLMQGASFRLHW 367

BLAST of Spg005157 vs. ExPASy Swiss-Prot
Match: B4FHU1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.8e-132
Identity = 242/361 (67.04%), Postives = 279/361 (77.29%), Query Frame = 0

Query: 21  PHRRHR--RPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRRRFAAEASVGDSESGGSTP 80
           PHRR    RP    L P  I AP  PL        S V    R A     G     G   
Sbjct: 17  PHRRPHLARPLCPTLNP--IRAPLPPL--------SRVLSHARPARAVGGGIEPKEGVVA 76

Query: 81  VSDDGG----FVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGGVGKAFV 140
             D+ G     VGED+AAF+L +Q +ASWAYF  ILG VL  LNV+WID S  GVG  F+
Sbjct: 77  EGDESGGGPVLVGEDSAAFELKDQSVASWAYFAGILGAVLVALNVLWIDPST-GVGTKFL 136

Query: 141 DAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTV 200
           DAV+ +SDSHEVVML L  IFA+VHSG+ASLR+ GEK+VGER +RVLFAG+SLPLAV+T+
Sbjct: 137 DAVASVSDSHEVVMLLLTIIFAVVHSGMASLRESGEKIVGERVYRVLFAGISLPLAVTTI 196

Query: 201 VYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWET 260
           VYFINHRYDG QLWQ+QGI G+H+L+W SSFISFFFLYPSTFNLLEVAAVDKPK+H+WET
Sbjct: 197 VYFINHRYDGTQLWQVQGITGIHELLWFSSFISFFFLYPSTFNLLEVAAVDKPKLHMWET 256

Query: 261 GIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADF 320
           GI+RITRHPQMVGQV+WCLAHT+WIGNSVAVAAS+GLI HHLFG WNGDRRL  RYG  F
Sbjct: 257 GIMRITRHPQMVGQVIWCLAHTLWIGNSVAVAASVGLISHHLFGAWNGDRRLLSRYGEAF 316

Query: 321 EAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAYLAHPLMQAASYRLH 376
           E +K+RTSV+PFAAI+DGRQKLP DY+KEF RLPY++IT LT+GAY AHPLMQA+SY+L 
Sbjct: 317 EVLKKRTSVMPFAAIIDGRQKLPKDYHKEFFRLPYVAITMLTLGAYFAHPLMQASSYQLP 366

BLAST of Spg005157 vs. ExPASy TrEMBL
Match: A0A6J1I3V9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470740 PE=4 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.3e-193
Identity = 342/375 (91.20%), Postives = 356/375 (94.93%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA +LF SPFP+SS+QPH RHRRPDA  LRP SIS PNF  QL FSLNSSP+ RRR
Sbjct: 1   MAVASATTLFFSPFPTSSNQPHHRHRRPDAFTLRPSSISTPNFLPQLSFSLNSSPIPRRR 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEAS+GDSESGGST  SDD G VGEDAA F+LSEQKLASW YFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQL  IPG+HQLVWLSSFISFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHTVWIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYGADFEAVKRRTSVVPFAAI+DGRQKLPDDYYKEFIRLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 375

BLAST of Spg005157 vs. ExPASy TrEMBL
Match: A0A6J1F2X1 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439180 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.7e-192
Identity = 340/375 (90.67%), Postives = 355/375 (94.67%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA +LF  P P+SS+QPH RHRR DA  LRP SIS PNF  QLPFSLNSSP+ RRR
Sbjct: 1   MAVASATTLFFPPLPTSSNQPHHRHRRLDAFTLRPSSISTPNFLPQLPFSLNSSPIPRRR 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RF+AEAS+GDSESGGST  SDD G VGEDAA F+LSEQKLASWAYFTVILGVVLFVLNVV
Sbjct: 61  RFSAEASIGDSESGGSTSTSDDDGLVGEDAAVFNLSEQKLASWAYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAFVDA+SGLSDSHEVVMLALIFIFA+VHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFVDAISGLSDSHEVVMLALIFIFAVVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQL  IPG+HQLVWLSSFISFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLHSIPGLHQLVWLSSFISFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGI+RITRHPQMVGQV+WCLAHTVWIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIMRITRHPQMVGQVIWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYGADFEAVKRRTSVVPFAAI+DGRQKLPDDYYKEFIRLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAILDGRQKLPDDYYKEFIRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 375

BLAST of Spg005157 vs. ExPASy TrEMBL
Match: A0A1S3BYR9 (15-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494534 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.4e-183
Identity = 328/375 (87.47%), Postives = 345/375 (92.00%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA   FS P+ SS      RHRRPDAI+LRPFSISAPN  LQLP SLNS  + RR 
Sbjct: 1   MAVASATPPFSPPYLSSP-----RHRRPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRP 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFAAEAS+GD ESGGST VSDD G VGEDAA FDLSEQKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RFAAEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DAVSG+SDSHEVVML L  IFAIVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQ +PG+HQLVWLSSF+SFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGIIRITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVIWCLAHTIWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYG DFEAV+RRTS++PFAAIVDGRQKLPDDYYKEF+RLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGVDFEAVQRRTSIIPFAAIVDGRQKLPDDYYKEFLRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 370

BLAST of Spg005157 vs. ExPASy TrEMBL
Match: A0A0A0KXF1 (NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.0e-183
Identity = 328/375 (87.47%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA   FS PF SS   P  RHRRPDAI+ RPFSIS PN   QLP SLNS  + RR 
Sbjct: 1   MAVASATPPFSPPFLSS---PRHRHRRPDAISFRPFSISTPNCLQQLPLSLNSFHIFRRP 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           R  AEAS+GD ESGGST VSDD GFVGEDAAAFDLSEQKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RLVAEASIGDRESGGSTSVSDDEGFVGEDAAAFDLSEQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DAVSG+SDSHEVVML LIFIFAIVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLIFIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQ +P +HQLVWLSSF+SF FLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPLLHQLVWLSSFVSFIFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGIIRITRHPQMVGQV+WCLAHT+WIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVIWCLAHTIWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYGADFEAVK RTS++PFAAIVDGRQKLPDDYYKEF+RLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGADFEAVKSRTSIIPFAAIVDGRQKLPDDYYKEFLRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQAAS+RLHW
Sbjct: 361 LAHPLMQAASFRLHW 372

BLAST of Spg005157 vs. ExPASy TrEMBL
Match: A0A5A7TMA2 (15-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003540 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.7e-179
Identity = 324/375 (86.40%), Postives = 340/375 (90.67%), Query Frame = 0

Query: 1   MAVASAASLFSSPFPSSSSQPHRRHRRPDAIALRPFSISAPNFPLQLPFSLNSSPVSRRR 60
           MAVASA   FS P+ SS      RHRRPDAI+LRPFSISAPN  LQLP SLNS  + RR 
Sbjct: 1   MAVASATPPFSPPYLSSP-----RHRRPDAISLRPFSISAPNCLLQLPLSLNSLHIFRRP 60

Query: 61  RFAAEASVGDSESGGSTPVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVV 120
           RFAAEAS+GD ESGGST VSDD G VGEDAA FDLSEQKL SW YFTVILGVVLFVLNVV
Sbjct: 61  RFAAEASIGDRESGGSTSVSDDEGLVGEDAAVFDLSEQKLTSWVYFTVILGVVLFVLNVV 120

Query: 121 WIDNSAGGVGKAFVDAVSGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRV 180
           WIDNSAGGVGKAF+DAVSG+SDSHEVVML L  IFAIVHSGLASLRDQGEKLVGERAFRV
Sbjct: 121 WIDNSAGGVGKAFLDAVSGISDSHEVVMLLLTLIFAIVHSGLASLRDQGEKLVGERAFRV 180

Query: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLE 240
           LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQ +PG+HQLVWLSSF+SFFFLYPSTFNLLE
Sbjct: 181 LFAGVSLPLAVSTVVYFINHRYDGVQLWQLQSVPGLHQLVWLSSFVSFFFLYPSTFNLLE 240

Query: 241 VAAVDKPKMHLWETGIIRITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVW 300
           VAAVDKPKMHLWETGIIRITRHP    QV+WCLAHT+WIGNSVAVAASIGLIGHHLFGVW
Sbjct: 241 VAAVDKPKMHLWETGIIRITRHP----QVIWCLAHTIWIGNSVAVAASIGLIGHHLFGVW 300

Query: 301 NGDRRLAKRYGADFEAVKRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAY 360
           NGDRRLAKRYG DFEAVKRRTS++PFAAIVDGRQKLPDDYYKEF+RLPY+SITALTIGAY
Sbjct: 301 NGDRRLAKRYGVDFEAVKRRTSIIPFAAIVDGRQKLPDDYYKEFLRLPYLSITALTIGAY 360

Query: 361 LAHPLMQAASYRLHW 376
           LAHPLMQ AS+RLHW
Sbjct: 361 LAHPLMQVASFRLHW 366

BLAST of Spg005157 vs. TAIR 10
Match: AT1G10830.1 (15-cis-zeta-carotene isomerase )

HSP 1 Score: 495.0 bits (1273), Expect = 5.4e-140
Identity = 243/358 (67.88%), Postives = 291/358 (81.28%), Query Frame = 0

Query: 28  PDAIALRPFSISAPNFPL--QLP--------FSLNSSPVSRRRRFAAEASVGDSESGGST 87
           P ++ L P S   PN  L  ++P         SL SS     R+    +++ + +   S 
Sbjct: 11  PPSLLLLPPSPRRPNLTLIRRIPAHPRLGNSTSLLSSSSPVIRKILVRSTLREDQPIASD 70

Query: 88  PVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGGVGKAFVDAV 147
             S     +GED+AAF+L +QKL SW YF V+LGVVLF+LNVVWIDNS  G GK+F+DAV
Sbjct: 71  SESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNST-GFGKSFIDAV 130

Query: 148 SGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVYF 207
           S +S S EV ML LI IFAIVHSGLASLRD GEKL+GERAFRVLFAG+SLPLA+ST+VYF
Sbjct: 131 SNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMSTIVYF 190

Query: 208 INHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGII 267
           INHRYDG QLWQLQG+PGVH+ +W+++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETGI+
Sbjct: 191 INHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIM 250

Query: 268 RITRHPQMVGQVMWCLAHTVWIGNSVAVAASIGLIGHHLFGVWNGDRRLAKRYGADFEAV 327
           RITRHPQMVGQ++WCLAHT+WIGN+VA +AS+GLI HHLFG WNGDRRLAKRYG DFE++
Sbjct: 251 RITRHPQMVGQIVWCLAHTLWIGNTVAASASLGLIAHHLFGAWNGDRRLAKRYGEDFESI 310

Query: 328 KRRTSVVPFAAIVDGRQKLPDDYYKEFIRLPYISITALTIGAYLAHPLMQAASYRLHW 376
           K+RTSV+PFAAI +GRQ LP+DYYKEF+RLPY++ITALT+GAY AHPLMQ AS+RLHW
Sbjct: 311 KKRTSVIPFAAIFEGRQVLPEDYYKEFVRLPYLAITALTVGAYFAHPLMQGASFRLHW 367

BLAST of Spg005157 vs. TAIR 10
Match: AT1G10830.2 (15-cis-zeta-carotene isomerase )

HSP 1 Score: 302.0 bits (772), Expect = 6.7e-82
Identity = 159/256 (62.11%), Postives = 193/256 (75.39%), Query Frame = 0

Query: 28  PDAIALRPFSISAPNFPL--QLP--------FSLNSSPVSRRRRFAAEASVGDSESGGST 87
           P ++ L P S   PN  L  ++P         SL SS     R+    +++ + +   S 
Sbjct: 11  PPSLLLLPPSPRRPNLTLIRRIPAHPRLGNSTSLLSSSSPVIRKILVRSTLREDQPIASD 70

Query: 88  PVSDDGGFVGEDAAAFDLSEQKLASWAYFTVILGVVLFVLNVVWIDNSAGGVGKAFVDAV 147
             S     +GED+AAF+L +QKL SW YF V+LGVVLF+LNVVWIDNS  G GK+F+DAV
Sbjct: 71  SESSPTLLIGEDSAAFELGKQKLVSWVYFGVVLGVVLFILNVVWIDNST-GFGKSFIDAV 130

Query: 148 SGLSDSHEVVMLALIFIFAIVHSGLASLRDQGEKLVGERAFRVLFAGVSLPLAVSTVVYF 207
           S +S S EV ML LI IFAIVHSGLASLRD GEKL+GERAFRVLFAG+SLPLA+ST+VYF
Sbjct: 131 SNISGSPEVAMLMLILIFAIVHSGLASLRDIGEKLIGERAFRVLFAGISLPLAMSTIVYF 190

Query: 208 INHRYDGVQLWQLQGIPGVHQLVWLSSFISFFFLYPSTFNLLEVAAVDKPKMHLWETGII 267
           INHRYDG QLWQLQG+PGVH+ +W+++F+SFFFLYPSTFNLLEVAAVDKPKMHLWETGI+
Sbjct: 191 INHRYDGSQLWQLQGVPGVHEAIWVANFVSFFFLYPSTFNLLEVAAVDKPKMHLWETGIM 250

Query: 268 RITRHPQMVGQVMWCL 274
           RITRHPQ++   + CL
Sbjct: 251 RITRHPQVLNISLMCL 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022972107.16.8e-19391.2015-cis-zeta-carotene isomerase, chloroplastic [Cucurbita maxima][more]
XP_022932715.19.8e-19290.6715-cis-zeta-carotene isomerase, chloroplastic [Cucurbita moschata] >KAG6597644.1... [more]
XP_038882546.12.2e-19190.6715-cis-zeta-carotene isomerase, chloroplastic [Benincasa hispida][more]
XP_023540701.14.9e-19190.4515-cis-zeta-carotene isomerase, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG7029085.14.3e-18784.1615-cis-zeta-carotene isomerase, chloroplastic [Cucurbita argyrosperma subsp. arg... [more]
Match NameE-valueIdentityDescription
Q9SAC07.6e-13967.8815-cis-zeta-carotene isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
B4FHU11.8e-13267.0415-cis-zeta-carotene isomerase, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I3V93.3e-19391.2015-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1F2X14.7e-19290.6715-cis-zeta-carotene isomerase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A1S3BYR92.4e-18387.4715-cis-zeta-carotene isomerase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0KXF19.0e-18387.47NnrU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015720 PE=4 S... [more]
A0A5A7TMA22.7e-17986.4015-cis-zeta-carotene isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
Match NameE-valueIdentityDescription
AT1G10830.15.4e-14067.8815-cis-zeta-carotene isomerase [more]
AT1G10830.26.7e-8262.1115-cis-zeta-carotene isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.120.1630coord: 141..326
e-value: 1.8E-12
score: 49.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR3598815-CIS-ZETA-CAROTENE ISOMERASE, CHLOROPLASTICcoord: 48..375
IPR009915NnrU domainPFAMPF07298NnrUcoord: 147..366
e-value: 2.7E-51
score: 174.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg005157.1Spg005157.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016120 carotene biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0090471 9,15,9'-tri-cis-zeta-carotene isomerase activity