Spg004936 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004936
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
Locationscaffold9: 20548160 .. 20552533 (-)
RNA-Seq ExpressionSpg004936
SyntenySpg004936
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGTTTCTTCATCCCCAGTCTCGCCATTCCTTTTCGAATTCTCAACTCGATCACAGCTTTCACACTTGTCGATTCTTCCTTTCGGCTTCTTATCATCCTGCAATTCGGTGGTGAAAAGGGTTTTTGTTTTGATCTTCATTCGATCTTCATCTCCATGTCGATGCTCAACTCCTTCTTCAACAAGGGCTTCAAAGCTGCCCAATGGTATCGCTTCTGTTTATTTTGATTTTCGTTTTCTTTTCATTTTCTCTTTCCTTGCTCTTTCAATTGTGTCGGTCACTGCTTTCATTGATTCATATGCGATTACAGAATTTCTGCTTTTGATTGTAATTTGCGCTTCTGTATTCAGTAAAACCCTGCTTAAATTGACAATTCCACGAATAAAGCTGCTGAGGAATCGGAGGGAGATTCAGCTTAAGCAGATGCGACGGGACATAGCTAAGCTTCTTGAAACAGGCCAAGAAGCTACGGCTCGCATTCGGGTATGTGTTACTTTCTGAAGTGCATTTCACCAATTTAATATCAAGAGGGGGAGGATAAGAAAAAAGGGAAAAAAATTTATGTCCTGAAAGTTTTTGTTGTCTTAATTAGATCTGCTTTTGCATTCAGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTTCCAATTATTGAAACACAAAGGTAAATTTTCCTAACGAAGTTATTCTTTATATGAGGATATTAAGTGTTTCTTGTGGTTATCTAAATGAACACGAGAAAGATCTGAATGCAAATAAGTTAAATAGATTACAGTATTTTCATGGACCCTTGCTCTTCAACCTGTAGGATTTGGTTTGAAACAGACAGCTCAAAAAGTCTTCACACGATATCTAATATTTGTGAATTTTTAAAAATGACAACGATAATAATGGAGAAATTGACACTAATGGGAAATGCTATGGCTTTTGTCTACTGCTTATTTCTTCTTCTTCTTCCTAATATTTTGATTAAAAATGTCTAAATGAGTATAGCTGTCCATGTTTGGCAATTATGAAACACAATTTATTCTGGGAAACATTTATGTTTTCGATATGAACATGACTGACAAAACTAGGATGTTAGATCTTTTCAATGTTTCTAAATTTGAAATCAATACTGGACTTTTCTACAAGAGTTGTATTGTATAATGGTTTTTTTTTAGTCTTTTTATGAACATTTAATTATCTAGATTAGATATGTATATTTCTTTTGGAATTTCCTTCACAAATTGTTACCAAGAAGCTGGACCATTTCATGCCTACAACCAACCGAACTTGGAACATTGGATTTGTTTGTTGGACTTATGTACAAGTATAATTTTTTAAGTATTTTATTTACGAGGATTTAATTCTATGATTAGATGTGTATATATTTGGTTCGAGATTTCCCTCATAAATTGTGACTGAGGAGCTGCATCATATAGAAATATGTAATTGATGTCAAAGTTTCTCACTGATGTTGTATCTACAGTCCATTCTTTCCCATTATTGAATGAACTGAATCTGTCCAATTGTTTTTGTCCTTACTCCTTGTCTATGAGAAAATGGCTTGTGGCTCTCTCTTTCTTGGGTTGGGTTATTGTCATGTTTGTAAAACTCATCAGTAGTATCCATATTTGTGCTCTTTTGGTTGATAAGAATGAGCATTAGAAATATGCTGGTATTTCACTTTAAAAGTTTGAACAGATATTTGCACATAAAGATAGAATATTGGAGAATATGTGCTCCTTTGAGGAATATTTTCCCATATTGAAGAATATTCTTTCATCCTAGTTTGGGATATTGGAGATCCATCCCAAAATTATGGAAGTTAAGTCTTAGATTGAAAAATTGTTAATTATAACTACAGCTAGTTGTCCAGCCTGATCATCCAACTTGTCCTGCGAATGACTCAAAACATCAAGCCTTGCAAACTGGAAATTTAGTTGCAATTGATTATGCTTAAAGGGTGGATGCTAAACTTACAAGAAACTGATTAGTTTGCACTTTCAAATTCACAAAATTTTTGGGTTAATCACCAAGCTTTCCATTTTTGGTGAAAATCCATGTCGTCTTTTGGATAACAGCTTTTGTCCCAAGTTACCTGAAATTTAAAACTACATGTCCAAATTGTATCCTTACTGTAAAATCTTTTGAATCTTACTCCATCTTTATTGTAGAGAATGCTTGCATTTCCATGCATTTCTTCCTTTGTTCAATTGGTGCTTAATTAGTTGCAGTATCTTGATATTTCATATGGTCCCATTTTTATTTCATCCTGTCCAATGATATCTTGGTATCAATATTTATAAATACCATATATCTTTCTACGTTTTTTTTTATCATTCTGATTTCACTCTGTTGCTTATGGTACTTGTATTGAGACATTTGTTCCTTCTACTTTATGCTTGTCCCAAATATCATTGTAAACTCCCAAATTTTTCAGGGAATGTCCTCTAGACTTGAAAGAAGCTATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGCGCTGATCTTACAGAGCTTCTCCAGGTTCAGATGCATTTTGCTGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAATCGTCAGGTTCTTAGAATTTATTGTTTATGTTTGATCAGAAAGAAAGCTTTACTGCAGTCGATAGGATTATATATTTCCTTTTAATTTTGGTTGCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATTGCTGAAGAGCACGACTTAGATTGGGATCCTGCTGGAACGGAAGCTGAGTTTAACAAATCTCCTGAAGATTTGCTTGTAAGTGTCGTTCCTTTTTGTCTCAACTTGTGGTGACTCTTCATGTCTTAGTTTCTGTTCTCTCTCTTCACAGAATGGATCGGCGCAGTTTGTCAGTGCATCAAAATTACCCCTTCCTAAAGAGAAACATGACGAAACGTATAATGCTACTCCTGATTTGGCTAGTACACCACAACCTGATTCTGATTCAGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATCTCCACATCCTCCTACTGTAGAGGCTGGACCTGCATCATCACTGATCCCACCTCCATCTGTGCCACTACCGCCGCCGGAATCTGATCATGATTCATTCAAGTATTCTGGTTTTCCTGAAATCCCACAAGATTTACACCTAAGACATGATGAGGCGACATTGGTGAGATCTGTTTCTCCCAGCAACCATGAAATGAATATCTCTGTTGGTGAAGATAAACAGTTTTTGCCTTTTATTACTACCCCATCATTGTCATCTTCGTTTTCTCATAGACAAACTGATTTGTCGCCACCCTCTGAAACAAGAACTCCCGAGGAGAAGTTTGGTTTTGAACCAAAGCTCGAACCCGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCCTTTCAAACCACAGTTTGAACAGGAAATAAATTCACCACCACCCTCTGCTTCAAGGACAAAGAGTGAGGTCAATGTGGATATCAGTGTGGATTTGCAGGATGTATTGGCCGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTCTTGCAAAGGTTAGAATCGACGAGCTCACAAAGAAAAAGAACGATCATGACCCTGAAATTAATTGCGAGAATCCATTCCATGGAGCTACTGCAAGTCCACCAGACCATAAAGAAACATTCCAACTAAACCAACAAGATTCTCCGGGGCATCATTCTAGTTCTATGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAGGTAAGCCGGAATTCAGATGTTTCTGATCACCACCAGCTCGAGGAACAGAAATCGGGTTTCGATTCCTCGCCCGGTGATTCACCATCTGAACGTATAGTACATCCTCATCAACCCCAGAGGCTTCCATCAATGGATGACGACCCGTACTTTTCATACCCAAATTTATTTACATCACAGAAACCAAATCTGGGATCTGATCATTCATCTGGTGGTGGTTCCTGATCTACCCATGAGGGTTGAGTTGAATTACATGCCATGCCGTCTAGGCTGGGGCCTCGTGAATTAATATTTCGTTCAGCTTTCATAACGAGTTTTCAATATCCTCTAGAGCTATAGAAGCGAGAATTCAATGTACAAAACAGTAAAGATATATTGTACAATCAGGATTGGTCTCTTGTTATGTACATTTTTCGGCCTATAGTTTGTATTTATTTGATTATTTCAGATGTCACTAATATAATGCTATAAATCATTTTAGGGCTGGATCTAAAAAGCTCGG

mRNA sequence

TCAGTTTCTTCATCCCCAGTCTCGCCATTCCTTTTCGAATTCTCAACTCGATCACAGCTTTCACACTTGTCGATTCTTCCTTTCGGCTTCTTATCATCCTGCAATTCGGTGGTGAAAAGGGTTTTTGTTTTGATCTTCATTCGATCTTCATCTCCATGTCGATGCTCAACTCCTTCTTCAACAAGGGCTTCAAAGCTGCCCAATGGTATCGCTTCTGTTTATTTTGATTTTCGTTTTCTTTTCATTTTCTCTTTCCTTGCTCTTTCAATTGTGTCGGTCACTGCTTTCATTGATTCATATGCGATTACAGAATTTCTGCTTTTGATTGTAATTTGCGCTTCTGTATTCAGTAAAACCCTGCTTAAATTGACAATTCCACGAATAAAGCTGCTGAGGAATCGGAGGGAGATTCAGCTTAAGCAGATGCGACGGGACATAGCTAAGCTTCTTGAAACAGGCCAAGAAGCTACGGCTCGCATTCGGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTTCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCTATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGCGCTGATCTTACAGAGCTTCTCCAGGTTCAGATGCATTTTGCTGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAATCGTCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATTGCTGAAGAGCACGACTTAGATTGGGATCCTGCTGGAACGGAAGCTGAGTTTAACAAATCTCCTGAAGATTTGCTTAATGGATCGGCGCAGTTTGTCAGTGCATCAAAATTACCCCTTCCTAAAGAGAAACATGACGAAACGTATAATGCTACTCCTGATTTGGCTAGTACACCACAACCTGATTCTGATTCAGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATCTCCACATCCTCCTACTGTAGAGGCTGGACCTGCATCATCACTGATCCCACCTCCATCTGTGCCACTACCGCCGCCGGAATCTGATCATGATTCATTCAAGTATTCTGGTTTTCCTGAAATCCCACAAGATTTACACCTAAGACATGATGAGGCGACATTGGTGAGATCTGTTTCTCCCAGCAACCATGAAATGAATATCTCTGTTGGTGAAGATAAACAGTTTTTGCCTTTTATTACTACCCCATCATTGTCATCTTCGTTTTCTCATAGACAAACTGATTTGTCGCCACCCTCTGAAACAAGAACTCCCGAGGAGAAGTTTGGTTTTGAACCAAAGCTCGAACCCGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCCTTTCAAACCACAGTTTGAACAGGAAATAAATTCACCACCACCCTCTGCTTCAAGGACAAAGAGTGAGGTCAATGTGGATATCAGTGTGGATTTGCAGGATGTATTGGCCGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTCTTGCAAAGGTTAGAATCGACGAGCTCACAAAGAAAAAGAACGATCATGACCCTGAAATTAATTGCGAGAATCCATTCCATGGAGCTACTGCAAGTCCACCAGACCATAAAGAAACATTCCAACTAAACCAACAAGATTCTCCGGGGCATCATTCTAGTTCTATGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAGGTAAGCCGGAATTCAGATGTTTCTGATCACCACCAGCTCGAGGAACAGAAATCGGGTTTCGATTCCTCGCCCGGTGATTCACCATCTGAACGTATAGTACATCCTCATCAACCCCAGAGGCTTCCATCAATGGATGACGACCCGTACTTTTCATACCCAAATTTATTTACATCACAGAAACCAAATCTGGGATCTGATCATTCATCTGGTGGTGGTTCCTGA

Coding sequence (CDS)

TCAGTTTCTTCATCCCCAGTCTCGCCATTCCTTTTCGAATTCTCAACTCGATCACAGCTTTCACACTTGTCGATTCTTCCTTTCGGCTTCTTATCATCCTGCAATTCGGTGGTGAAAAGGGTTTTTGTTTTGATCTTCATTCGATCTTCATCTCCATGTCGATGCTCAACTCCTTCTTCAACAAGGGCTTCAAAGCTGCCCAATGGTATCGCTTCTGTTTATTTTGATTTTCGTTTTCTTTTCATTTTCTCTTTCCTTGCTCTTTCAATTGTGTCGGTCACTGCTTTCATTGATTCATATGCGATTACAGAATTTCTGCTTTTGATTGTAATTTGCGCTTCTGTATTCAGTAAAACCCTGCTTAAATTGACAATTCCACGAATAAAGCTGCTGAGGAATCGGAGGGAGATTCAGCTTAAGCAGATGCGACGGGACATAGCTAAGCTTCTTGAAACAGGCCAAGAAGCTACGGCTCGCATTCGGGTAGAGCATATAATCAGAGAAGAGAATATGATGGCTGCTCAGGAAATTCTTGAGTTGTTTTGTGAGCTTATTGTTGTTCGTCTTCCAATTATTGAAACACAAAGGGAATGTCCTCTAGACTTGAAAGAAGCTATCTCAAGTGTGTGTTTTGCTGCTCCAAGGTGCGCTGATCTTACAGAGCTTCTCCAGGTTCAGATGCATTTTGCTGCTAAATATGGGAAGGAATTTGTATCAGCTGCAACTGAGCTCATGCCTAACTGTGGTGTTAATCGTCAGTTGATAGAGCTGCTTTCTGTTCGTGCTCCTTCACCTGAAAAGAAACTGAAGCTCTTAAAGGAAATTGCTGAAGAGCACGACTTAGATTGGGATCCTGCTGGAACGGAAGCTGAGTTTAACAAATCTCCTGAAGATTTGCTTAATGGATCGGCGCAGTTTGTCAGTGCATCAAAATTACCCCTTCCTAAAGAGAAACATGACGAAACGTATAATGCTACTCCTGATTTGGCTAGTACACCACAACCTGATTCTGATTCAGAATTGGACATGTTAGACTTTCCTGAAGTTCCAAAGATGTCAGTATCTCCACATCCTCCTACTGTAGAGGCTGGACCTGCATCATCACTGATCCCACCTCCATCTGTGCCACTACCGCCGCCGGAATCTGATCATGATTCATTCAAGTATTCTGGTTTTCCTGAAATCCCACAAGATTTACACCTAAGACATGATGAGGCGACATTGGTGAGATCTGTTTCTCCCAGCAACCATGAAATGAATATCTCTGTTGGTGAAGATAAACAGTTTTTGCCTTTTATTACTACCCCATCATTGTCATCTTCGTTTTCTCATAGACAAACTGATTTGTCGCCACCCTCTGAAACAAGAACTCCCGAGGAGAAGTTTGGTTTTGAACCAAAGCTCGAACCCGAGATAAATTCACCACCACCCTCTGTTTCAAGGACCCCTTTCAAACCACAGTTTGAACAGGAAATAAATTCACCACCACCCTCTGCTTCAAGGACAAAGAGTGAGGTCAATGTGGATATCAGTGTGGATTTGCAGGATGTATTGGCCGCTGCTCAGGCTGCTGCTGAAACTGCTGAACGTGCTGCTGCTGCAGCTCGCTCCGCAGCTAGTCTTGCAAAGGTTAGAATCGACGAGCTCACAAAGAAAAAGAACGATCATGACCCTGAAATTAATTGCGAGAATCCATTCCATGGAGCTACTGCAAGTCCACCAGACCATAAAGAAACATTCCAACTAAACCAACAAGATTCTCCGGGGCATCATTCTAGTTCTATGCCATATTATTCACAAGATTCTTTCCAAGAACATCACAAGGTAAGCCGGAATTCAGATGTTTCTGATCACCACCAGCTCGAGGAACAGAAATCGGGTTTCGATTCCTCGCCCGGTGATTCACCATCTGAACGTATAGTACATCCTCATCAACCCCAGAGGCTTCCATCAATGGATGACGACCCGTACTTTTCATACCCAAATTTATTTACATCACAGAAACCAAATCTGGGATCTGATCATTCATCTGGTGGTGGTTCCTGA

Protein sequence

SVSSSPVSPFLFEFSTRSQLSHLSILPFGFLSSCNSVVKRVFVLIFIRSSSPCRCSTPSSTRASKLPNGIASVYFDFRFLFIFSFLALSIVSVTAFIDSYAITEFLLLIVICASVFSKTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEFVSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEIPQDLHLRHDEATLVRSVSPSNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPPPSVSRTPFKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS
Homology
BLAST of Spg004936 vs. NCBI nr
Match: XP_038884985.1 (uncharacterized protein LOC120075564 [Benincasa hispida])

HSP 1 Score: 890.6 bits (2300), Expect = 8.7e-255
Identity = 484/570 (84.91%), Postives = 510/570 (89.47%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQM FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAE+H+LDW+PA TEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEDHNLDWNPAETEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLP+E H+E YNATPDLAS+PQPDSDSELDMLDFPEVPK+SV P 
Sbjct: 198 DLLNGSAQFVSASKLPLPQENHNEAYNATPDLASSPQPDSDSELDMLDFPEVPKISVCPQ 257

Query: 358 PPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDLHLRHDEATLVRSVSPSN 417
           P T +AG ASS++PPPSVP P  E++H S KYS  PE  PQDLHLRH+E T VRSVSPS 
Sbjct: 258 PNT-DAGSASSMMPPPSVP-PASETNHASLKYSDIPETPPQDLHLRHEEVTSVRSVSPST 317

Query: 418 HEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPP 477
            +MNISVGEDKQFLPFIT PSLSSSFSHRQTD  PPS++  PEEKFGF+P++E EINSP 
Sbjct: 318 DQMNISVGEDKQFLPFITPPSLSSSFSHRQTDFLPPSDSTAPEEKFGFKPRIEQEINSPL 377

Query: 478 PSVSRTP-----FKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERA 537
           PSVSRT      FKP+FE EIN+PP S SRT SEVN D+ VDLQDVLAAAQAAAETAERA
Sbjct: 378 PSVSRTAEEKFGFKPRFEPEINAPPSSVSRTMSEVNEDVGVDLQDVLAAAQAAAETAERA 437

Query: 538 AAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHH 597
           AAAARSAASLAKVRIDELTKKKND DPEI+CENPFHGATAS PDHKETFQLNQQD  GHH
Sbjct: 438 AAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASSPDHKETFQLNQQDYLGHH 497

Query: 598 SSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPS 657
           SSSMPYYSQDSFQE H VS + DVS+HHQ E +K GFDSSPGDSPS  I  PHQPQRLPS
Sbjct: 498 SSSMPYYSQDSFQERHIVSHSLDVSEHHQPEPEKLGFDSSPGDSPSAHIAVPHQPQRLPS 557

Query: 658 MDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           MDDDPYFSYPNLFT QKPN GSDH S GGS
Sbjct: 558 MDDDPYFSYPNLFTPQKPNPGSDHPSAGGS 585

BLAST of Spg004936 vs. NCBI nr
Match: XP_023521005.1 (uncharacterized protein LOC111784587 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 888.6 bits (2295), Expect = 3.3e-254
Identity = 492/598 (82.27%), Postives = 519/598 (86.79%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSELD LDFPEVPK+SVS H
Sbjct: 198 DLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSELDTLDFPEVPKISVSLH 257

Query: 358 PPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQDLHLRHDEATLVRSVSP 417
           PPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQDLHLRH+E   VRSVSP
Sbjct: 258 PPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQDLHLRHEE---VRSVSP 317

Query: 418 SNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
           SN  +NISVGEDKQFLPFIT PSLSSSFSHRQTDL P S+++TPEEK GF+P+ E EINS
Sbjct: 318 SNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKTPEEKLGFKPRFEQEINS 377

Query: 478 PPPSVSRT-------------------------------PFKPQFEQEINSPPPSASRTK 537
           PPPSVSRT                                FKPQFEQ ++SPPPS SRTK
Sbjct: 378 PPPSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQNLDSPPPSVSRTK 437

Query: 538 SEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCE 597
           SEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND D E++CE
Sbjct: 438 SEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCE 497

Query: 598 NPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEE 657
           NPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EHH+VS +SDV+ HHQLE 
Sbjct: 498 NPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEHHRVSCDSDVTGHHQLEP 557

Query: 658 QKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSD SSGGGS
Sbjct: 558 QKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGS 606

BLAST of Spg004936 vs. NCBI nr
Match: XP_022981875.1 (uncharacterized protein LOC111480885 [Cucurbita maxima])

HSP 1 Score: 885.2 bits (2286), Expect = 3.6e-253
Identity = 492/598 (82.27%), Postives = 518/598 (86.62%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSELD LDFPEVPK+SVS H
Sbjct: 198 DLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSELDTLDFPEVPKISVSLH 257

Query: 358 PPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQDLHLRHDEATLVRSVSP 417
           PPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQDLHLRH+E   VRSVSP
Sbjct: 258 PPTTFDAATAQPLIPPPSVS-PPPETDCDSFKSSGIIPEIPPQDLHLRHEE---VRSVSP 317

Query: 418 SNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
           SN  +NISVGEDKQFLPFIT PSLSSSFSHRQTDL P S+++TPEEKFGF+P+ E EINS
Sbjct: 318 SNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKTPEEKFGFKPRFEQEINS 377

Query: 478 PPPSVSRT-------------------------------PFKPQFEQEINSPPPSASRTK 537
           PP SVSRT                                FKPQFEQ ++SPPPS SRTK
Sbjct: 378 PPLSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQNLDSPPPSVSRTK 437

Query: 538 SEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCE 597
           SEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND D E++CE
Sbjct: 438 SEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCE 497

Query: 598 NPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEE 657
           NPFHGA  +PPDHK+TF   QQDS G +  SMPYYSQDSF+EHH+VS +SDV+ HHQLE 
Sbjct: 498 NPFHGAAVTPPDHKQTF---QQDSLGDY--SMPYYSQDSFKEHHRVSCDSDVTGHHQLEP 557

Query: 658 QKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSD SSGGGS
Sbjct: 558 QKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGS 606

BLAST of Spg004936 vs. NCBI nr
Match: KAG6607619.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 883.2 bits (2281), Expect = 1.4e-252
Identity = 490/598 (81.94%), Postives = 517/598 (86.45%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSELD LDFPEVPK+SVS H
Sbjct: 198 DLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSELDTLDFPEVPKISVSLH 257

Query: 358 PPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQDLHLRHDEATLVRSVSP 417
           PPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQDLHLRH+E   VRSVSP
Sbjct: 258 PPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQDLHLRHEE---VRSVSP 317

Query: 418 SNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
           SN  +NISVGEDKQFLPFIT PSLSSS SHRQTDL P S+++TPEEK GF+P+ E EINS
Sbjct: 318 SNDHLNISVGEDKQFLPFITPPSLSSSSSHRQTDLCPSSDSKTPEEKLGFKPRFEQEINS 377

Query: 478 PPPSVSRT-------------------------------PFKPQFEQEINSPPPSASRTK 537
           PPPSVSRT                                FKPQFEQ ++SPPPS SR K
Sbjct: 378 PPPSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQNLDSPPPSVSRIK 437

Query: 538 SEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCE 597
           SEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND D E++CE
Sbjct: 438 SEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCE 497

Query: 598 NPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEE 657
           NPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EHH+VS +SDV+ HHQLE 
Sbjct: 498 NPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEHHRVSCDSDVTGHHQLEP 557

Query: 658 QKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSD SSGGGS
Sbjct: 558 QKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGS 606

BLAST of Spg004936 vs. NCBI nr
Match: XP_022926259.1 (uncharacterized protein LOC111433438 [Cucurbita moschata])

HSP 1 Score: 877.5 bits (2266), Expect = 7.6e-251
Identity = 486/598 (81.27%), Postives = 517/598 (86.45%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSELD LDFPEVPK+SVS H
Sbjct: 198 DLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSELDPLDFPEVPKISVSLH 257

Query: 358 PPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQDLHLRHDEATLVRSVSP 417
           PPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQDLHL+ +E   VRSVSP
Sbjct: 258 PPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQDLHLKREE---VRSVSP 317

Query: 418 SNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
           SN  +N+SVGEDKQFLPFIT PSLSSSFSHRQTD+ P S+++TPEEKFGF+P+ E EINS
Sbjct: 318 SNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEKFGFKPRFEQEINS 377

Query: 478 PPPSVSRT-------------------------------PFKPQFEQEINSPPPSASRTK 537
           PPPSVSRT                                FKPQFEQ ++SPPPS SRTK
Sbjct: 378 PPPSVSRTHEERNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQNLDSPPPSVSRTK 437

Query: 538 SEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCE 597
           SEVNVD+SV+LQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND D E++CE
Sbjct: 438 SEVNVDVSVNLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCE 497

Query: 598 NPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEE 657
           NPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EHH+VS + DV+ HHQLE 
Sbjct: 498 NPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEHHRVSCDLDVTGHHQLEP 557

Query: 658 QKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           QKSGFDSSP +SPSERI HPHQ QRLPSMDDDPYFSYPNLFTSQKPNLGSD SSGGGS
Sbjct: 558 QKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGS 606

BLAST of Spg004936 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.9e-19
Identity = 61/170 (35.88%), Postives = 102/170 (60.00%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           K  LKL + RI++L+N++   ++  +R++A+LL    E +ARIRVE IIR+E ++   +I
Sbjct: 14  KVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQI 73

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           +E+ CEL+  R+ +I    E PL++KE+I ++ +++ R   + EL Q++    AKYGK  
Sbjct: 74  IEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQR-IQIPELEQIKNQLKAKYGKGL 133

Query: 238 VSAATELMPNC----GVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW 284
            + A     NC     VN +++  LS   P P    + L EIAE+ ++DW
Sbjct: 134 ENEA-----NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of Spg004936 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-18
Identity = 62/166 (37.35%), Postives = 101/166 (60.84%), Query Frame = 0

Query: 121 LKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILEL 180
           L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ ++ A EILEL
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 181 FCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHFAAKYGKEFVS 240
           +C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V     AKY KE+  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGK 134

Query: 241 AATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 286
                     VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 135 LCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Spg004936 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-18
Identity = 62/166 (37.35%), Postives = 101/166 (60.84%), Query Frame = 0

Query: 121 LKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILEL 180
           L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ ++ A EILEL
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 181 FCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHFAAKYGKEFVS 240
           +C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V     AKY KE+  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGK 134

Query: 241 AATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 286
                     VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 135 LCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Spg004936 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.1e-18
Identity = 62/166 (37.35%), Postives = 101/166 (60.84%), Query Frame = 0

Query: 121 LKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILEL 180
           L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ ++ A EILEL
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 181 FCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHFAAKYGKEFVS 240
           +C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V     AKY KE+  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGK 134

Query: 241 AATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 286
                     VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 135 LCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Spg004936 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 7.1e-18
Identity = 61/166 (36.75%), Postives = 100/166 (60.24%), Query Frame = 0

Query: 121 LKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEILEL 180
           L+L I R+KLL  ++    ++ R++IA  L  G++  ARIRVEHIIRE+ ++ A EILEL
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 181 FCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRC-ADLTELLQVQMHFAAKYGKEFVS 240
           +C+L++ R  +I++ +E    L E++S++ +AAPR  +++ EL  V     AKY K +  
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKGYGK 134

Query: 241 AATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDP 286
                     VN +L+  LSV AP      + L EIA+ +++ ++P
Sbjct: 135 LCRTNQIGT-VNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 179

BLAST of Spg004936 vs. ExPASy TrEMBL
Match: A0A6J1J0W1 (uncharacterized protein LOC111480885 OS=Cucurbita maxima OX=3661 GN=LOC111480885 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.8e-253
Identity = 492/598 (82.27%), Postives = 518/598 (86.62%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSELD LDFPEVPK+SVS H
Sbjct: 198 DLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSELDTLDFPEVPKISVSLH 257

Query: 358 PPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQDLHLRHDEATLVRSVSP 417
           PPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQDLHLRH+E   VRSVSP
Sbjct: 258 PPTTFDAATAQPLIPPPSVS-PPPETDCDSFKSSGIIPEIPPQDLHLRHEE---VRSVSP 317

Query: 418 SNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
           SN  +NISVGEDKQFLPFIT PSLSSSFSHRQTDL P S+++TPEEKFGF+P+ E EINS
Sbjct: 318 SNDHLNISVGEDKQFLPFITPPSLSSSFSHRQTDLCPSSDSKTPEEKFGFKPRFEQEINS 377

Query: 478 PPPSVSRT-------------------------------PFKPQFEQEINSPPPSASRTK 537
           PP SVSRT                                FKPQFEQ ++SPPPS SRTK
Sbjct: 378 PPLSVSRTHEERNSLSPSVSRTHEEINSLPPSVSPEEKFGFKPQFEQNLDSPPPSVSRTK 437

Query: 538 SEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCE 597
           SEVNVD+SVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND D E++CE
Sbjct: 438 SEVNVDVSVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCE 497

Query: 598 NPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEE 657
           NPFHGA  +PPDHK+TF   QQDS G +  SMPYYSQDSF+EHH+VS +SDV+ HHQLE 
Sbjct: 498 NPFHGAAVTPPDHKQTF---QQDSLGDY--SMPYYSQDSFKEHHRVSCDSDVTGHHQLEP 557

Query: 658 QKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           QKSGFDSSP +SPSERI HPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSD SSGGGS
Sbjct: 558 QKSGFDSSPSNSPSERIAHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGS 606

BLAST of Spg004936 vs. ExPASy TrEMBL
Match: A0A6J1EEM3 (uncharacterized protein LOC111433438 OS=Cucurbita moschata OX=3662 GN=LOC111433438 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.7e-251
Identity = 486/598 (81.27%), Postives = 517/598 (86.45%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FAAKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLPK+KHDETY+ATPDL S PQPDSDSELD LDFPEVPK+SVS H
Sbjct: 198 DLLNGSAQFVSASKLPLPKDKHDETYSATPDLGSAPQPDSDSELDPLDFPEVPKISVSLH 257

Query: 358 PPTV-EAGPASSLIPPPSVPLPPPESDHDSFKYSG-FPEI-PQDLHLRHDEATLVRSVSP 417
           PPT  +A  A  LIPPPSV  PPPE+D DSFK SG  PEI PQDLHL+ +E   VRSVSP
Sbjct: 258 PPTTFDAATAQPLIPPPSVS-PPPETDRDSFKSSGIIPEIPPQDLHLKREE---VRSVSP 317

Query: 418 SNHEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
           SN  +N+SVGEDKQFLPFIT PSLSSSFSHRQTD+ P S+++TPEEKFGF+P+ E EINS
Sbjct: 318 SNDHLNVSVGEDKQFLPFITPPSLSSSFSHRQTDVCPSSDSKTPEEKFGFKPRFEQEINS 377

Query: 478 PPPSVSRT-------------------------------PFKPQFEQEINSPPPSASRTK 537
           PPPSVSRT                                FKPQFEQ ++SPPPS SRTK
Sbjct: 378 PPPSVSRTHEERNSLSPSVSRTHEETNSLRPSVSPEEKFGFKPQFEQNLDSPPPSVSRTK 437

Query: 538 SEVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCE 597
           SEVNVD+SV+LQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKND D E++CE
Sbjct: 438 SEVNVDVSVNLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDRDAEVSCE 497

Query: 598 NPFHGATASPPDHKETFQLNQQDSPGHHSSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEE 657
           NPFHGA  +PPDHK+TF   QQDS G++  SMPYYSQDSF+EHH+VS + DV+ HHQLE 
Sbjct: 498 NPFHGAAVTPPDHKQTF---QQDSLGNY--SMPYYSQDSFKEHHRVSCDLDVTGHHQLEP 557

Query: 658 QKSGFDSSPGDSPSERIVHPHQPQRLPSMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           QKSGFDSSP +SPSERI HPHQ QRLPSMDDDPYFSYPNLFTSQKPNLGSD SSGGGS
Sbjct: 558 QKSGFDSSPSNSPSERIAHPHQTQRLPSMDDDPYFSYPNLFTSQKPNLGSDRSSGGGS 606

BLAST of Spg004936 vs. ExPASy TrEMBL
Match: A0A0A0KBZ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 3.8e-248
Identity = 473/571 (82.84%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTEL+QVQM F AKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELIQVQMLFGAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           +SAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW+PA TEAEFNKSPE
Sbjct: 138 LSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGS QFV ASKLPLP+EKH+ET N T DLAS PQPDSDSELDMLDFPEVPKMSV P 
Sbjct: 198 DLLNGSTQFVGASKLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEVPKMSVCPQ 257

Query: 358 PPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDLHLRHDEATLVRSVSPSN 417
            PT++AG A S+IPPPSV  PP E+DH SF+YSG PE  PQ+LH RH+E TLVRSVSPSN
Sbjct: 258 -PTIDAGSAPSMIPPPSVS-PPRETDHASFEYSGIPESPPQNLHSRHEEVTLVRSVSPSN 317

Query: 418 HEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPP 477
            +MN+SVGEDKQFLPFIT PSLS SFS RQT+LSP S+  TPEEKF  +P++  EINSP 
Sbjct: 318 DQMNVSVGEDKQFLPFITPPSLSPSFSRRQTELSPSSDPTTPEEKFDIKPRIREEINSPL 377

Query: 478 PSVSRTP-----FKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERA 537
           PSVSR       FKP+FE EINS P S SRT SEVN D+ VDLQDVLAAAQAAAETAERA
Sbjct: 378 PSVSRASEEKYGFKPRFEPEINSTPSSVSRTMSEVNADLGVDLQDVLAAAQAAAETAERA 437

Query: 538 AAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHH 597
           AAAARSAASLAKVRIDELTKKKND DPEI+CENPFHG TASP DH ET++LNQQDS G+H
Sbjct: 438 AAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGGTASPADHTETYKLNQQDSLGYH 497

Query: 598 SSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSE-RIVHPHQPQRLP 657
           SS+MPYYSQDSFQEHHK+SR+ +VSDH QL+ +KS FD SPG+SPSE + V PHQPQRLP
Sbjct: 498 SSAMPYYSQDSFQEHHKLSRDPEVSDHSQLKPEKSSFDYSPGNSPSEHQTVAPHQPQRLP 557

Query: 658 SMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           SMDDDPYFSYPNLFTSQKPN G DHSS GGS
Sbjct: 558 SMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 586

BLAST of Spg004936 vs. ExPASy TrEMBL
Match: A0A1S4E4M8 (uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 2.7e-246
Identity = 475/571 (83.19%), Postives = 502/571 (87.92%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTEL+QVQM F AKYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELIQVQMLFGAKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDW+PA TEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWNPAETEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNG   FV  SKLPLP+EKH+ET N T DLAS PQPDSDSELDMLDFPEVPKMSV P 
Sbjct: 198 DLLNG---FVGTSKLPLPQEKHNETSNTTTDLASGPQPDSDSELDMLDFPEVPKMSVCPQ 257

Query: 358 PPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDLHLRHDEATLVRSVSPSN 417
            PT++AG A S+I PPSV  P PE+D  SFKYSG PE  PQ+LH RH E TLVRSVSPSN
Sbjct: 258 -PTIDAGSAPSMILPPSVS-PQPETDRASFKYSGIPESPPQNLHSRHGEVTLVRSVSPSN 317

Query: 418 HEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPP 477
            +MN+SVGEDKQFLPFIT PSLS SFSHRQTDLSPPS+  TPEEKF  +P++  EINSP 
Sbjct: 318 DQMNVSVGEDKQFLPFITPPSLSPSFSHRQTDLSPPSDPTTPEEKFDIKPRITEEINSPL 377

Query: 478 PSVSRTP-----FKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERA 537
           PSVSR       FKP+F+ EINS P S SRT  EVN D+SVDLQDVLAAAQAAAETAERA
Sbjct: 378 PSVSRASEEQFGFKPRFDSEINSTPSSVSRTMCEVNADLSVDLQDVLAAAQAAAETAERA 437

Query: 538 AAAARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPGHH 597
           AAAARSAASLAKVRIDELTKKKND DPEI+CENPFHGATASP  H ET++LNQQDS GHH
Sbjct: 438 AAAARSAASLAKVRIDELTKKKNDKDPEISCENPFHGATASPAGHTETYKLNQQDSLGHH 497

Query: 598 SSSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSE-RIVHPHQPQRLP 657
           SSSMPYYSQDS QEHHK+SR+ +VSDH QLE +KS FDSSPG+SPSE + V PHQPQRLP
Sbjct: 498 SSSMPYYSQDSSQEHHKLSRDPEVSDHSQLEPEKSSFDSSPGNSPSEHQTVAPHQPQRLP 557

Query: 658 SMDDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           SMDDDPYFSYPNLFTSQKPN G DHSS GGS
Sbjct: 558 SMDDDPYFSYPNLFTSQKPNPGFDHSSAGGS 583

BLAST of Spg004936 vs. ExPASy TrEMBL
Match: A0A6J1EU69 (uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436655 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 3.3e-236
Identity = 462/569 (81.20%), Postives = 483/569 (84.89%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI
Sbjct: 18  KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQ+ FA KYGKEF
Sbjct: 78  LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQLLFAVKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAATELMPNCGVNRQL+ELLSVRAPSPEKKLKLLKEIAEEHD+DWDPAGTEAEFNKSPE
Sbjct: 138 VSAATELMPNCGVNRQLVELLSVRAPSPEKKLKLLKEIAEEHDVDWDPAGTEAEFNKSPE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLLNGSAQFVSASKLPLP+EKH+E YNA       PQPDSDSELDMLDFPEVPK+SVSPH
Sbjct: 198 DLLNGSAQFVSASKLPLPQEKHNEAYNA-------PQPDSDSELDMLDFPEVPKISVSPH 257

Query: 358 PPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEI-PQDLHLRHDEATLVRSVSPSN 417
             T++ G ASS+IPPPS   PPPE+ HDS KYS  PE  P+DL LR  E T VRSVSPS 
Sbjct: 258 -STIDPGSASSMIPPPSTS-PPPETTHDSSKYSCIPETPPEDLQLRRGEVTPVRSVSPSK 317

Query: 418 HEMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINSPP 477
            +MNISVGEDKQFLPFI  PS                  RTPEEKF F+P+ EPEINSPP
Sbjct: 318 DQMNISVGEDKQFLPFIAPPS------------------RTPEEKFSFKPQFEPEINSPP 377

Query: 478 PSVSRTP---FKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAA 537
           PSVSRTP   F  ++EQEI SPPPSASRTKS    D+S+DLQDVLAAAQAA ETAERAAA
Sbjct: 378 PSVSRTPDEKFGLKYEQEIISPPPSASRTKS----DVSLDLQDVLAAAQAAVETAERAAA 437

Query: 538 AARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATASPPDHKETFQLNQQDSPG-HHS 597
           AARSAASL KVRID LT K ND  PEI CENPFHGAT SPPDH++TF LNQQDS G HHS
Sbjct: 438 AARSAASLTKVRIDTLTMKTNDQGPEICCENPFHGATTSPPDHEQTFHLNQQDSIGHHHS 497

Query: 598 SSMPYYSQDSFQEHHKVSRNSDVSDHHQLEEQKSGFDSSPGDSPSERIVHPHQPQRLPSM 657
           SSMPYYSQDSFQEHHK S  SDVSDHH+ E QKSGFDSSPGDSPSE I  PHQPQRLPSM
Sbjct: 498 SSMPYYSQDSFQEHHKESPGSDVSDHHRFESQKSGFDSSPGDSPSEHITIPHQPQRLPSM 555

Query: 658 DDDPYFSYPNLFTSQKPNLGSDHSSGGGS 682
           DDDPYFSYPNLFTSQKPN G DHSS  GS
Sbjct: 558 DDDPYFSYPNLFTSQKPNPGFDHSSADGS 555

BLAST of Spg004936 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 396.0 bits (1016), Expect = 6.2e-110
Identity = 303/655 (46.26%), Postives = 366/655 (55.88%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARIRVEHIIREE MMAAQEI
Sbjct: 18  KTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLTEL QVQ+ F +KYGKEF
Sbjct: 78  LELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           V+AA+EL P+ GVNR+L+ELLSVRAPSPE KLKLLKEIAEEH+LDWDPA TE +  KS E
Sbjct: 138 VAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHE 197

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNATPDLASTPQPDSDSELDMLDFPEVPKMSVSPH 357
           DLL+G  QF   SKLPLP+E++++T N T   A+  + DSDSE D+LDFPEVP + + P 
Sbjct: 198 DLLDGPKQFGGGSKLPLPEEQNEKT-NLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPT 257

Query: 358 PPTVEAGPASSLIPPPSVPLPPPESDHDSFKYSGFPEIPQDLHLRHDEATLVRSVSPSNH 417
           P       A+S+  P            D+ K + +     DL    + A + ++ S  + 
Sbjct: 258 P------GATSVNAP------------DAAKSASYEHTSHDLPFDSENAGVEKTASKRDE 317

Query: 418 ---EMNISVGEDKQFLPFITTPSLSSSFSHRQTDLSPPSETRTPEEKFGFEPKLEPEINS 477
              + + +V E +Q     ++P L  SF  +                           N 
Sbjct: 318 HPAKASKTVVEGQQ-----SSPILMESFEKK---------------------------NY 377

Query: 478 PPPSVSRTPFKPQFEQEINSPPPSASRTKSEVNVDISVDLQDVLAAAQAAAETAERAAAA 537
            PPS+      P  E         ASR       D   DLQDVL AAQAAA++AERAA+A
Sbjct: 378 SPPSIDAVGPIPTKES-------GASRDTPRKISD--GDLQDVLMAAQAAADSAERAASA 437

Query: 538 ARSAASLAKVRIDELTKKKNDHDPEINCENPFHGATAS--PPDHK--------------- 597
           ARSAASLA++RI+ELT+K +D  PE   ENPFH  +      DH+               
Sbjct: 438 ARSAASLAQLRINELTRKTSDQYPESPSENPFHAPSMGNLQFDHQNSSASSSGDLTELQR 497

Query: 598 -ETFQL-----NQQDSPGHHSSSMP-------------------YYSQDSFQEHHKVSRN 657
            ET  L     N Q    H S   P                   ++S +  Q  H+ S  
Sbjct: 498 AETSSLFNSEQNNQQPQTHSSMEKPQFDRQNSSFSSYGDLTPQRFHSMEKPQFDHQNSSG 557

Query: 658 S-----------------------------DVSDHHQLEEQKSGFD------SSPGD--- 682
           S                             D++ H     +K  FD      SS GD   
Sbjct: 558 SNYDDLTPQRFSSLEKPQFDHQNSSGSNYDDLTPHRFPSMEKPQFDHQNSSVSSYGDLPE 608

BLAST of Spg004936 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 381.7 bits (979), Expect = 1.2e-105
Identity = 304/685 (44.38%), Postives = 366/685 (53.43%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KTLLKLTIPRIKL+RNRRE Q+KQMRR+IAKLLETGQEATARIRVEHIIREE MMAAQEI
Sbjct: 18  KTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMMAAQEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCELI VRLPIIE QRECPLDLKEAISSVCFAAPRC+DLTEL QVQ+ F +KYGKEF
Sbjct: 78  LELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSKYGKEF 137

Query: 238 VSAATELMPNCGVNR------------------------------QLIELLSVRAPSPEK 297
           V+AA+EL P+ GVNR                              QL+ELLSVRAPSPE 
Sbjct: 138 VAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQLVELLSVRAPSPET 197

Query: 298 KLKLLKEIAEEHDLDWDPAGTEAEFNKSPEDLLNGSAQFVSASKLPLPKEKHDETYNATP 357
           KLKLLKEIAEEH+LDWDPA TE +  KS EDLL+G  QF   SKLPLP+E++++T N T 
Sbjct: 198 KLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKT-NLTS 257

Query: 358 DLASTPQPDSDSELDMLDFPEVPKMSVSPHPPTVEAGPASSLIPPPSVPLPPPESDHDSF 417
             A+  + DSDSE D+LDFPEVP + + P P       A+S+  P            D+ 
Sbjct: 258 LSAAKEKSDSDSEYDILDFPEVPNVLLRPTP------GATSVNAP------------DAA 317

Query: 418 KYSGFPEIPQDLHLRHDEATLVRSVSPSNH---EMNISVGEDKQFLPFITTPSLSSSFSH 477
           K + +     DL    + A + ++ S  +    + + +V E +Q     ++P L  SF  
Sbjct: 318 KSASYEHTSHDLPFDSENAGVEKTASKRDEHPAKASKTVVEGQQ-----SSPILMESFEK 377

Query: 478 RQTDLSPPSETRTPEEKFGFEPKLEPEINSPPPSVSRTPFKPQFEQEINSPPPSASRTKS 537
           +                           N  PPS+      P  E         ASR   
Sbjct: 378 K---------------------------NYSPPSIDAVGPIPTKES-------GASRDTP 437

Query: 538 EVNVDISVDLQDVLAAAQAAAETAERAAAAARSAASLAKVRIDELTKKKNDHDPEINCEN 597
               D   DLQDVL AAQAAA++AERAA+AARSAASLA++RI+ELT+K +D  PE   EN
Sbjct: 438 RKISD--GDLQDVLMAAQAAADSAERAASAARSAASLAQLRINELTRKTSDQYPESPSEN 497

Query: 598 PFHGATAS--PPDHK----------------ETFQL-----NQQDSPGHHSSSMP----- 657
           PFH  +      DH+                ET  L     N Q    H S   P     
Sbjct: 498 PFHAPSMGNLQFDHQNSSASSSGDLTELQRAETSSLFNSEQNNQQPQTHSSMEKPQFDRQ 557

Query: 658 --------------YYSQDSFQEHHKVSRNS----------------------------- 682
                         ++S +  Q  H+ S  S                             
Sbjct: 558 NSSFSSYGDLTPQRFHSMEKPQFDHQNSSGSNYDDLTPQRFSSLEKPQFDHQNSSGSNYD 617

BLAST of Spg004936 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 231.5 bits (589), Expect = 2.0e-60
Identity = 121/209 (57.89%), Postives = 160/209 (76.56%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KT  K+ + RIKL+RN+R + +KQMRRDIA LL++GQ+ATARIRVEH+IRE+N+ AA EI
Sbjct: 32  KTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAANEI 91

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           +ELFCELIV RL II  Q++CP+DLKE I+S+ FAAPRC+++ EL  ++  FA KYGK+F
Sbjct: 92  IELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYGKDF 151

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           VSAAT+L P+CGVNR LI+ LSVR P  E KLK++KEIA+E  +DWD   TE E  K  E
Sbjct: 152 VSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELLKPQE 211

Query: 298 DLLNGSAQFVSASKLPLPKEKHDETYNAT 327
           + ++G  +FVSAS LP+ +   +E  + T
Sbjct: 212 ESIDGPRKFVSASSLPVNRAAINEPIDPT 240

BLAST of Spg004936 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 217.2 bits (552), Expect = 4.0e-56
Identity = 109/188 (57.98%), Postives = 148/188 (78.72%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KT L L I R+KLL+N+R++QLK M+++IA  L+ GQE  ARIRVEH+IRE N+ AA EI
Sbjct: 18  KTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMNLWAAYEI 77

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           LELFCE I+ R+PI+E+++ECP +L+EAI+S+ FAAPRC+++ +LLQ++  F  KYGKEF
Sbjct: 78  LELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFGTKYGKEF 137

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
           +  A+EL P+ GVNR +IE LS  +PS   +LK+LKEIA+E+ L+WD + TEAEF KS E
Sbjct: 138 IMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATEAEFMKSHE 197

Query: 298 DLLNGSAQ 306
           DLL G+ Q
Sbjct: 198 DLLGGAKQ 205

BLAST of Spg004936 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 189.1 bits (479), Expect = 1.2e-47
Identity = 96/195 (49.23%), Postives = 145/195 (74.36%), Query Frame = 0

Query: 118 KTLLKLTIPRIKLLRNRREIQLKQMRRDIAKLLETGQEATARIRVEHIIREENMMAAQEI 177
           KT L++   R+K+L+N++EIQ+KQ+RR++A+LLE+GQ  TARIRVEH++REE  +AA E+
Sbjct: 15  KTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTVAAYEL 74

Query: 178 LELFCELIVVRLPIIETQRECPLDLKEAISSVCFAAPRCADLTELLQVQMHFAAKYGKEF 237
           + ++CEL+VVRL +IE+Q+ CP+DLKEA++SV FA+ R +D+ EL ++   F  KYGK+F
Sbjct: 75  IGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTKYGKDF 134

Query: 238 VSAATELMPNCGVNRQLIELLSVRAPSPEKKLKLLKEIAEEHDLDWDPAGTEAEFNKSPE 297
            ++A EL P+ GV+R L+E LS +AP    K+K+L  IAEEH++ W+ A +  E +    
Sbjct: 135 STSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWE-AQSFVESDPKDT 194

Query: 298 DLLNGSAQFVSASKL 313
           +LLNG+  F  AS +
Sbjct: 195 ELLNGANSFQPASSM 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884985.18.7e-25584.91uncharacterized protein LOC120075564 [Benincasa hispida][more]
XP_023521005.13.3e-25482.27uncharacterized protein LOC111784587 [Cucurbita pepo subsp. pepo][more]
XP_022981875.13.6e-25382.27uncharacterized protein LOC111480885 [Cucurbita maxima][more]
KAG6607619.11.4e-25281.94IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022926259.17.6e-25181.27uncharacterized protein LOC111433438 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q54I392.9e-1935.88IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Q3ZBV11.1e-1837.35IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
P539901.1e-1837.35IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX001.1e-1837.35IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q5R6G87.1e-1836.75IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J0W11.8e-25382.27uncharacterized protein LOC111480885 OS=Cucurbita maxima OX=3661 GN=LOC111480885... [more]
A0A6J1EEM33.7e-25181.27uncharacterized protein LOC111433438 OS=Cucurbita moschata OX=3662 GN=LOC1114334... [more]
A0A0A0KBZ03.8e-24882.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G020110 PE=3 SV=1[more]
A0A1S4E4M82.7e-24683.19uncharacterized protein LOC103502259 OS=Cucumis melo OX=3656 GN=LOC103502259 PE=... [more]
A0A6J1EU693.3e-23681.20uncharacterized protein LOC111436655 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G34220.26.2e-11046.26Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.11.2e-10544.38Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.12.0e-6057.89Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.14.0e-5657.98Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.11.2e-4749.23Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 132..159
NoneNo IPR availableCOILSCoilCoilcoord: 518..538
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 604..625
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..681
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 436..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 576..603
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 660..681
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 365..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 483..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 436..453
NoneNo IPR availablePANTHERPTHR12161:SF62REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 117..674
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 118..281
e-value: 1.0E-58
score: 197.9
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 117..674
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 113..289
e-value: 3.0E-75
score: 253.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004936.1Spg004936.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
molecular_function GO:0003676 nucleic acid binding