Spg004748 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004748
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCCHC-type domain-containing protein
Locationscaffold5: 20515473 .. 20518146 (-)
RNA-Seq ExpressionSpg004748
SyntenySpg004748
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTAATACCGGAAGGGAATCACATGTTTATTTCTCGCTTAGGCATCAATATTCTGTCGATTCATTGTTATGCGCTACATTAATATCTTATCTGTCGCATGTGGTTGCTGCGGCGAAAGTCTTGATAGCTCATCATCCACCGTATCTTTCTATCGCATTATTATTACTTTTCTTTATCTCATGTTCGACCTGTCGCATCCATATTTTATTCTTAGCACAACATCATTAGAATAAGATTTAATTTTAGATATTTTCTCTACTCGCCCCTTCCCTGTGGATTCGACCCTGGAATACTCCAGGTATTACTTGGTAGTGCATAATTTATACTTGGATTAATGCACAACAACGATATATTCTTTAGCTAATTCAAGATAATTAGAAGCGATTAAATTTCCCAACCAAGTTTTTGGCGCCGTTGCCGGGGAAGGCAACAGTAGTTTAGAACGTCTTTTATTATTTCTTATTCTGTGTATTGTGCTATTACAACCATTTGCCTTGAGTGGGTATTTTTCAGGTGTGTTAGGTGTGCACCCAAGAAATGGCTGCGCTGAACCCACAAAATCCTCCCGTAAATCCAATACAATTGGCTGATGATAGAGAACGTGTAAACCCGAGACTTTCCCTTAGATAATTTTCTCTTATCATCTATTGTGGTTTCATTGTTGAAGGAAAATGGCAAGCATTTAGTTCAAGTTGTTTTTTTTTAACCTTGGAGTTTATTTAGAGAAAAGGAAAATGAAAGGAAAGGAAAAGAAGAAAGGAAAAGAAATGGGAAAGGAAAGAAAGCCAAAAAAAAAAAGAAAAAAAAAGAAAAGAAAAGAAAGGAAAGTGGGAAAAGACAGCAGGAACCCACCACCGCCTAAGCCAACTCTTGCCCATTTTTAGCCCACTACCAGCCCATTTCTCATCATTTTTCATTCCAAAAGTTACAGAGCACTTGAGGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGGAAAAGCTCATGCAAAGCCAGCCATTGCAGAATCTGGGTGCAAAACTGTCAACCTCTGGTTTTTTGTTCGGTTTGAGCTACACAGAAGGAACTAAGAGGTGAGGTTTGATTTTCTTGAACGTTAGTTCATTTTATAACAAATGTCATGAAGGAAGCTTGAGCTAAATCGTATGAGAAGTTAATGGTTTTTGGAATGGAAAAAAGAGCACAGGATTTTCGAGCAAACCAGGAGGATCTCTGGTGTTCTCTGATGTTTTGAGTATTCTGGAGTGTACAGGTTGAATCAGAAGCTCTATTTGGTATGGTTGGAAAGCTTATTCAATTATCTACAACTTTCATGAAGAATTCGTGAGCTAAAAAGGACTTGAAGTAGGTTTAAATGCAGGAGGAAGATAAAGGGTAACATAGAAGCTCGAATCTGTGTTGTCTGATTTCGGGGAATTTCTGGACAAATTGGTTGGGAATCTCGTTTTCATTTCTTCAGGTAAGTTGTAGAGTGTTCGAAAATGAAGAGTTTGATGTGTATTTCATTAATTTTGGTTCAGTATTGAGAAAGTTGTGGGTGTTGGAAGTTAGGGTATAGAATCTGGAAAATCTGGAAAAGTAGAATGTGAATGTGATTCTTAATCCAATTGTTTAAAAATTCATGAGCATGGAAAGACAAAGCTATCTATGGTTCTAATGCTCGGTTTTGGTTATTTGACAGGCCAAGAGGAAGTAGGTCGAGTCTACAAACCAGGGAACTAGATAAGACTGTGAGTGACAAAATGAATTTCGAAAATGTTCTTAAACGGTTGTTCATGATTATATGTTTTACATGCGTTCCTATGAATGATACATGGTTTTTATGAACGGTTTCAAGCATGAGTTTTGAGCTCAGATTTTCTAATGAGGTTTATATACGAGCACGTGGCTAATGACTATATGAAAAGTAGCTGAAACGATATCTTTTAAAGCAAAGTATGGATTAGCAAGTACTTTTAAACGATTTCCAGTTTTTCATAAGCAAGCTGAGTAAGCTTAAGTTTTACGAAAGATAACGATAAGCAGGCATGTTTTAATGTTTTCATGTGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTCGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGAGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGATTGTACTGGGCGTGTCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATGTACTTGATATGCCTTGCTAGATTTTTAATGACGAACTTGTGAAATGTTTTAGAAAGCCTTTATCCTTACACGTTTATTTAAACGCTTTGACTGAGCATATTTATACGTTTAAAGGTTTATCACGTGTTTTGACTTTCAGATTTAGAGTTTTAAAATTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTCCACTTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCCATTCATATAGTAAATGTTTTGTAA

mRNA sequence

ATGTGTAATACCGGAAGGGAATCACATGTTTATTTCTCGCTTAGGCATCAATATTCTGTCGATTCATTGTTATGCGCTACATTAATATCTTATCTGTCGCATGTGGTTGCTGCGGCGAAAGTCTTGATAGCTCATCATCCACCAGCACTTGAGGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGGAAAAGCTCATGCAAAGCCAGCCATTGCAGAATCTGGGTGCAAAACTGTCAACCTCTGGTTTTTTGTTCGGTTTGAGCTACACAGAAGGAACTAAGAGGTTGAATCAGAAGCTCTATTTGGTAAGTTGTAGAGTGTTCGAAAATGAAGAGTTTGATGTGCCAAGAGGAAGTAGGTCGAGTCTACAAACCAGGGAACTAGATAAGACTGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCCATTCATATAGTAAATGTTTTGTAA

Coding sequence (CDS)

ATGTGTAATACCGGAAGGGAATCACATGTTTATTTCTCGCTTAGGCATCAATATTCTGTCGATTCATTGTTATGCGCTACATTAATATCTTATCTGTCGCATGTGGTTGCTGCGGCGAAAGTCTTGATAGCTCATCATCCACCAGCACTTGAGGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGGAAAAGCTCATGCAAAGCCAGCCATTGCAGAATCTGGGTGCAAAACTGTCAACCTCTGGTTTTTTGTTCGGTTTGAGCTACACAGAAGGAACTAAGAGGTTGAATCAGAAGCTCTATTTGGTAAGTTGTAGAGTGTTCGAAAATGAAGAGTTTGATGTGCCAAGAGGAAGTAGGTCGAGTCTACAAACCAGGGAACTAGATAAGACTGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCCATTCATATAGTAAATGTTTTGTAA

Protein sequence

MCNTGRESHVYFSLRHQYSVDSLLCATLISYLSHVVAAAKVLIAHHPPALEGESEGRRRRRISWFEVEEDREKLMQSQPLQNLGAKLSTSGFLFGLSYTEGTKRLNQKLYLVSCRVFENEEFDVPRGSRSSLQTRELDKTVEIELPVPDTLPTSAESSRSSSSTWLELYIESIHIVNVL
Homology
[Close]
BLAST of Spg004748 vs. NCBI nr
Match: KAA0058280.1 (uncharacterized protein E6C27_scaffold274G006090 [Cucumis melo var. makuwa])

HSP 1 Score: 108.6 bits (270), Expect = 5.6e-20
Identity = 72/143 (50.35%), Postives = 82/143 (57.34%), Query Frame = 0 

Query: 75  MQSQPLQNLGAKLSTSGFLFGLSYTEGTK------------------------RLNQKLY 134
           MQSQPLQNLGA ++    LF LSY EGTK                        + NQKLY
Sbjct: 1   MQSQPLQNLGATVNLWFVLFSLSYPEGTKSTGFSSKPGGFLVFSDALSILEIHKSNQKLY 60

Query: 135 LV-SCRV--FENEEFDVPRGSRSSLQTRELDKT---------------VEIELPVPDTLP 176
           LV   R+   EN +  +PRG+R SLQTRELDKT               V IELPVPD LP
Sbjct: 61  LVLEVRISNLENLKSGMPRGNRLSLQTRELDKTVIFPLSTLSVLRDNNVVIELPVPDILP 120

[Close]
BLAST of Spg004748 vs. NCBI nr
Match: KAA0037581.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 107.8 bits (268), Expect = 9.6e-20
Identity = 73/139 (52.52%), Postives = 82/139 (58.99%), Query Frame = 0 

Query: 53   ESEGRRRRRISWFEVEEDREKLMQSQPLQNLGAKL------STSGF--------LF--GL 112
            ESEGRR RRISWFEVEEDR+K MQSQPLQNLGA +      S++GF        +F   L
Sbjct: 1617 ESEGRRIRRISWFEVEEDRKKFMQSQPLQNLGATVNLWLFCSSTGFSSKLRGFLVFSDAL 1676

Query: 113  SYTEGTKRLNQKLYLVSCRVFENEEFDVPRGSRSSLQTRELDKTVEIELPVPDTLPTSAE 172
            S  E   + NQKLYL                             VEIELPVPDTL TSAE
Sbjct: 1677 SILE-VHKSNQKLYL-----------------------------VEIELPVPDTLSTSAE 1725

Query: 173  SSRSSSSTWLELYIESIHI 176
            SSRS+SSTWLELY ES+H+
Sbjct: 1737 SSRSNSSTWLELYFESVHV 1725

[Close]
BLAST of Spg004748 vs. NCBI nr
Match: KAA0040420.1 (NBS-LRR type resistance protein [Cucumis melo var. makuwa])

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-19
Identity = 60/101 (59.41%), Postives = 68/101 (67.33%), Query Frame = 0 

Query: 75  MQSQPLQNLGAKLSTSGFLFGLSYTEGTKRLNQKLYLVSCRVFENEEFDVPRGSRSSLQT 134
           MQSQPLQNLGA ++    LF LSY EGTKR                    PRG+R SLQT
Sbjct: 1   MQSQPLQNLGATVNFWFVLFSLSYPEGTKR--------------------PRGNRPSLQT 60

Query: 135 RELDKTVEIELPVPDTLPTSAESSRSSSSTWLELYIESIHI 176
            +LDKTVEIELP+PDTLPT AES  S+SSTWLELY ES+H+
Sbjct: 61  GKLDKTVEIELPMPDTLPTFAESFGSNSSTWLELYFESVHV 81

[Close]
BLAST of Spg004748 vs. NCBI nr
Match: KAA0066795.1 (uncharacterized protein E6C27_scaffold271G001440 [Cucumis melo var. makuwa] >TYK27942.1 uncharacterized protein E5676_scaffold384G001370 [Cucumis melo var. makuwa])

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-19
Identity = 68/125 (54.40%), Postives = 76/125 (60.80%), Query Frame = 0 

Query: 75  MQSQPLQNLGAKLSTSGFLFGLSYTEGTK------------------------RLNQKLY 134
           MQSQPLQNLGA ++    LFGLSY E TK                        + +QKLY
Sbjct: 1   MQSQPLQNLGATVNLWFVLFGLSYLEETKSTGSSSKPAGVLVFSDALSMLEVHKSDQKLY 60

Query: 135 LVSCRVFENEEFDVPRGSRSSLQTRELDKTVEIELPVPDTLPTSAESSRSSSSTWLELYI 176
           LV            PRG+R SLQTRELDKT  IELPVPDTLPTSAESS S+SSTWLELY 
Sbjct: 61  LV-----------WPRGNRPSLQTRELDKT--IELPVPDTLPTSAESSGSNSSTWLELYF 112

[Close]
BLAST of Spg004748 vs. NCBI nr
Match: KAA0053207.1 (hypothetical protein E6C27_scaffold1192G00340 [Cucumis melo var. makuwa] >TYK00136.1 hypothetical protein E5676_scaffold2510G00220 [Cucumis melo var. makuwa])

HSP 1 Score: 104.8 bits (260), Expect = 8.1e-19
Identity = 76/161 (47.20%), Postives = 85/161 (52.80%), Query Frame = 0 

Query: 75  MQSQPLQNLGA-----------KLST--SGFLF---GLSYTEGTKRLNQKLYLVSCRVFE 134
           MQSQPLQNLGA           K S+   GFL     LS  E   +LNQKLYLVSC VFE
Sbjct: 1   MQSQPLQNLGATANLWLFYSSTKFSSKPGGFLVFSDALSILE-LYKLNQKLYLVSCIVFE 60

Query: 135 NEEF---------------------------DVPRGSRSSLQTRELDKT----------- 176
           NEEF                           ++PRG+R SLQT ELDKT           
Sbjct: 61  NEEFYVYFINFGYVLRKLWVLEVRISNLENLELPRGNRPSLQTGELDKTIIFPLSTLSVL 120

[Close]
BLAST of Spg004748 vs. ExPASy TrEMBL
Match: A0A5A7UT17 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006090 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.7e-20
Identity = 72/143 (50.35%), Postives = 82/143 (57.34%), Query Frame = 0 

Query: 75  MQSQPLQNLGAKLSTSGFLFGLSYTEGTK------------------------RLNQKLY 134
           MQSQPLQNLGA ++    LF LSY EGTK                        + NQKLY
Sbjct: 1   MQSQPLQNLGATVNLWFVLFSLSYPEGTKSTGFSSKPGGFLVFSDALSILEIHKSNQKLY 60

Query: 135 LV-SCRV--FENEEFDVPRGSRSSLQTRELDKT---------------VEIELPVPDTLP 176
           LV   R+   EN +  +PRG+R SLQTRELDKT               V IELPVPD LP
Sbjct: 61  LVLEVRISNLENLKSGMPRGNRLSLQTRELDKTVIFPLSTLSVLRDNNVVIELPVPDILP 120

[Close]
BLAST of Spg004748 vs. ExPASy TrEMBL
Match: A0A5A7T7M6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002250 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.6e-20
Identity = 73/139 (52.52%), Postives = 82/139 (58.99%), Query Frame = 0 

Query: 53   ESEGRRRRRISWFEVEEDREKLMQSQPLQNLGAKL------STSGF--------LF--GL 112
            ESEGRR RRISWFEVEEDR+K MQSQPLQNLGA +      S++GF        +F   L
Sbjct: 1617 ESEGRRIRRISWFEVEEDRKKFMQSQPLQNLGATVNLWLFCSSTGFSSKLRGFLVFSDAL 1676

Query: 113  SYTEGTKRLNQKLYLVSCRVFENEEFDVPRGSRSSLQTRELDKTVEIELPVPDTLPTSAE 172
            S  E   + NQKLYL                             VEIELPVPDTL TSAE
Sbjct: 1677 SILE-VHKSNQKLYL-----------------------------VEIELPVPDTLSTSAE 1725

Query: 173  SSRSSSSTWLELYIESIHI 176
            SSRS+SSTWLELY ES+H+
Sbjct: 1737 SSRSNSSTWLELYFESVHV 1725

[Close]
BLAST of Spg004748 vs. ExPASy TrEMBL
Match: A0A5D3DWP3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001370 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 6.1e-20
Identity = 68/125 (54.40%), Postives = 76/125 (60.80%), Query Frame = 0 

Query: 75  MQSQPLQNLGAKLSTSGFLFGLSYTEGTK------------------------RLNQKLY 134
           MQSQPLQNLGA ++    LFGLSY E TK                        + +QKLY
Sbjct: 1   MQSQPLQNLGATVNLWFVLFGLSYLEETKSTGSSSKPAGVLVFSDALSMLEVHKSDQKLY 60

Query: 135 LVSCRVFENEEFDVPRGSRSSLQTRELDKTVEIELPVPDTLPTSAESSRSSSSTWLELYI 176
           LV            PRG+R SLQTRELDKT  IELPVPDTLPTSAESS S+SSTWLELY 
Sbjct: 61  LV-----------WPRGNRPSLQTRELDKT--IELPVPDTLPTSAESSGSNSSTWLELYF 112

[Close]
BLAST of Spg004748 vs. ExPASy TrEMBL
Match: A0A5A7TAS6 (NBS-LRR type resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold35G00450 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 6.1e-20
Identity = 60/101 (59.41%), Postives = 68/101 (67.33%), Query Frame = 0 

Query: 75  MQSQPLQNLGAKLSTSGFLFGLSYTEGTKRLNQKLYLVSCRVFENEEFDVPRGSRSSLQT 134
           MQSQPLQNLGA ++    LF LSY EGTKR                    PRG+R SLQT
Sbjct: 1   MQSQPLQNLGATVNFWFVLFSLSYPEGTKR--------------------PRGNRPSLQT 60

Query: 135 RELDKTVEIELPVPDTLPTSAESSRSSSSTWLELYIESIHI 176
            +LDKTVEIELP+PDTLPT AES  S+SSTWLELY ES+H+
Sbjct: 61  GKLDKTVEIELPMPDTLPTFAESFGSNSSTWLELYFESVHV 81

[Close]
BLAST of Spg004748 vs. ExPASy TrEMBL
Match: A0A5A7UFD2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2510G00220 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.9e-19
Identity = 76/161 (47.20%), Postives = 85/161 (52.80%), Query Frame = 0 

Query: 75  MQSQPLQNLGA-----------KLST--SGFLF---GLSYTEGTKRLNQKLYLVSCRVFE 134
           MQSQPLQNLGA           K S+   GFL     LS  E   +LNQKLYLVSC VFE
Sbjct: 1   MQSQPLQNLGATANLWLFYSSTKFSSKPGGFLVFSDALSILE-LYKLNQKLYLVSCIVFE 60

Query: 135 NEEF---------------------------DVPRGSRSSLQTRELDKT----------- 176
           NEEF                           ++PRG+R SLQT ELDKT           
Sbjct: 61  NEEFYVYFINFGYVLRKLWVLEVRISNLENLELPRGNRPSLQTGELDKTIIFPLSTLSVL 120

The following BLAST results are available for this feature:
BLAST of Spg004748 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0058280.15.6e-2050.35uncharacterized protein E6C27_scaffold274G006090 [Cucumis melo var. makuwa][more]
KAA0037581.19.6e-2052.52reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0040420.11.2e-1959.41NBS-LRR type resistance protein [Cucumis melo var. makuwa][more]
KAA0066795.11.2e-1954.40uncharacterized protein E6C27_scaffold271G001440 [Cucumis melo var. makuwa] >TYK... [more]
KAA0053207.18.1e-1947.20hypothetical protein E6C27_scaffold1192G00340 [Cucumis melo var. makuwa] >TYK001... [more]
BLAST of Spg004748 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg004748 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UT172.7e-2050.35CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5A7T7M64.6e-2052.52Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5D3DWP36.1e-2054.40Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7TAS66.1e-2059.41NBS-LRR type resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A5A7UFD23.9e-1947.20Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
BLAST of Spg004748 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004748.1Spg004748.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:0016787 hydrolase activity