Spg004518 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg004518 vs. NCBI nr
Match: XP_022141862.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 766.1 bits (1977), Expect = 3.1e-217
Identity = 436/894 (48.77%), Postives = 573/894 (64.09%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           R  YDVF+SF  +DT N   G L+EALHQ+GI+ F  D+KL+   +  E L +KAI+ES+
Sbjct: 56  RMTYDVFISFRGEDTRNNFVGFLYEALHQLGIMTFMDDRKLLIGDDLSENL-MKAIEESR 115

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
           + IVVLSKDYASS  CL+EL K+M+  SET T +VLP+FYHVDPSHVRHQ+G+F+K F +
Sbjct: 116 SSIVVLSKDYASSKWCLKELVKIMDCMSET-THQVLPVFYHVDPSHVRHQSGNFKKSFDE 175

Query: 124 H-----------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALL 183
           H            + +  EV  W+D+M K+ NL+G VV   S E  I+KK+T+Q+F    
Sbjct: 176 HEEDAKKITGQQKEKYLVEVKNWKDSMTKIGNLTGEVVTQHSSEVNIVKKITNQIFERWR 235

Query: 184 SGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTT 243
             + TS++NLVG++    ++   L +  D+VRF+GIVGMGGIGK              TT
Sbjct: 236 PKIATSDKNLVGMTYQLLKMNVKLGLGLDDVRFVGIVGMGGIGK--------------TT 295

Query: 244 LAEAIYHKICDEFDGSCFLRV-SGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGV 303
           +A+ +Y  I   F+GSCFLRV  GS   NN+ SLQ QLLS +F  E     ++WDED G 
Sbjct: 296 IAKVVYDCIKSNFEGSCFLRVFGGSSKQNNLLSLQEQLLSRLFLKE---NVRMWDEDYGA 355

Query: 304 EEITRLHC-KKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIP 363
           E I    C +KVLLV+DG E+   +  LA +PDWFGPGSR+IITTRNK++L      EI 
Sbjct: 356 EMIKNQLCSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEIL--HQPYEII 415

Query: 364 TYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALR 423
            Y++  L+ D +LQLFC  AF  +      D+N+  L + I++    LPLALKV+GS L 
Sbjct: 416 EYKLKLLDADSALQLFCNRAFGID----YIDSNFMNLSKEILEKLGRLPLALKVIGSYLN 475

Query: 424 GRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELL 483
            +   IW+E L  L +V +K + ++LK+SYD L    Q++FLD+AC F G+  D+V E+L
Sbjct: 476 KKEEVIWKETLYRLIKVDEKNLFEVLKISYDGLGVESQKVFLDLACLFHGRKKDKVIEIL 535

Query: 484 GSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRD 543
             FG+     E++L M RCL+++   +I+MHDL+  +G+EIV RES TQ GKQSRIW ++
Sbjct: 536 ECFGYSP-YSELQLFMERCLLEVSPTKILMHDLVVALGQEIVHRESLTQPGKQSRIWFQE 595

Query: 544 DMKRVSVQQYGVEETRGIVLDLE-EGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLS 603
           D+ RV   ++G++ T GIVL+LE +  EL +EA   + MT LRILEI++V+L+  + +LS
Sbjct: 596 DVDRVFTVKHGLKYTEGIVLNLEKKQKELILEAKSFTDMTYLRILEISNVQLSGNINFLS 655

Query: 604 NLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLL 663
           NLLGF+NWPG PSKCLPP FQ   L+EL +P S I  LW+G K F  LK+ID+SDSK LL
Sbjct: 656 NLLGFINWPGYPSKCLPPNFQSRCLLELHLPLSSIEILWEGKKDFEKLKVIDVSDSKYLL 715

Query: 664 KTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTF-----CWECD- 723
            TPD S V N+E LVLR C  L  IH S+  LNRL  LDL +CS+ K+F     C   + 
Sbjct: 716 GTPDFSKVPNLERLVLRNCRKLYHIHPSINDLNRLRLLDLSNCSSFKSFSSHLRCKSLEG 775

Query: 724 -----------QKIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPS 783
                       K E  MEHL +L+L GT I+ LHSSIG L GLVLL+LR+C  LSSLPS
Sbjct: 776 LILSESGLTSSPKFEVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIMLSSLPS 835

Query: 784 NVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLR 843
             IG  KSL+ L+L GC  LD I  S  NA CLE LD+ +TS+ Q PP+IH   NLK L 
Sbjct: 836 E-IGNLKSLQVLLLEGCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENLKRLN 895

Query: 844 CQGLFNSIWHSL------------NSPSFGLLSLTSLDLSNCNLVDENIPQDLH 855
           C+GL   IWHSL            +   FGL SL  L L NCNLVDE+IP+DLH
Sbjct: 896 CEGLSRKIWHSLLPRDIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLH 922

BLAST of Spg004518 vs. NCBI nr
Match: XP_022141775.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 735.3 bits (1897), Expect = 5.9e-208
Identity = 419/895 (46.82%), Postives = 575/895 (64.25%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           R  YDVF+SF  +DT N   G L++AL Q+GI+ F  DKKL+   +  E+  +KAI+ES+
Sbjct: 41  RMTYDVFISFRGEDTRNNFVGFLYQALRQLGIMTFMDDKKLLIGDDLSEKF-VKAIEESR 100

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
           + IVVLSKDYASS  CL+EL K+M+  SET T RVLP+FYHVDPSHVRHQ+G+F+K F  
Sbjct: 101 SSIVVLSKDYASSKWCLKELAKIMDCMSET-THRVLPVFYHVDPSHVRHQSGNFKKSFDG 160

Query: 124 H-----------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALL 183
           H            + + +EV  W+D+M K+ NL+G VV   S E +++KK+T+Q+F    
Sbjct: 161 HEEKAPKLIGVQREEYLKEVQNWKDSMTKIGNLAGEVVTQHSSEVDVVKKITNQIFERWR 220

Query: 184 SGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTT 243
             + TS++NLVG++R   E+   L +  D+VRF+GIVGMGGIGK              TT
Sbjct: 221 PKIATSDKNLVGMTRQLLEMNVKLGLGLDDVRFVGIVGMGGIGK--------------TT 280

Query: 244 LAEAIYHKICDEFDGSCFLRV-SGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGV 303
           +A+ +Y  I  +F+GSCFLRV  GS    N+ SLQ QLLS +F  E     ++WDED G 
Sbjct: 281 IAKVVYDCITSKFEGSCFLRVFGGSSKQCNLVSLQEQLLSRLFLKE---NVRIWDEDYGA 340

Query: 304 EEITRLHC-KKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIP 363
           E I    C +KVLLV+D  E+   +  LA +PDWFGPGSR+IITTRNK++L      EI 
Sbjct: 341 EMIENQLCSRKVLLVLDEVEEKEQLEMLARSPDWFGPGSRIIITTRNKEIL--HQPYEIL 400

Query: 364 TYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALR 423
            Y++  L++D +LQLFC  AF  +      D+++  L + +V+    LPLALKV+GS L 
Sbjct: 401 EYKMKLLDDDSALQLFCNRAFGID----YIDSSFMNLSKGMVEKLGRLPLALKVIGSYLN 460

Query: 424 GRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELL 483
            + + IW+E L  L +V +K + ++L +SYD L A  Q++FLD+ACFF G+  D+V ++L
Sbjct: 461 KKDVLIWKETLNRLIKVDEKHLFEVLNISYDGLGAESQKVFLDLACFFNGRRTDKVIKIL 520

Query: 484 GSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRD 543
            SFG+     E+ L   +CLI++ + +I+MHDLI  +G+EIVR+ES TQ GKQSRIWL++
Sbjct: 521 ESFGYNP-HSELNLFTEKCLIEVSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQE 580

Query: 544 DMKRVSVQQYGVEETRGIVLDLE-EGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLS 603
           D+ R    ++G++ T GIVL+LE +  EL +EA   + MT LRILEI++V+L   ++ LS
Sbjct: 581 DVVRAFTMKHGLKYTEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLYGNIKSLS 640

Query: 604 NLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLL 663
           NLL  LNWPG PSKCLP  FQ   L+EL +  S I+++WDG K+F +LK+ID+SDS+ LL
Sbjct: 641 NLLALLNWPGYPSKCLPSNFQSGYLLELHLRHSSIVRIWDGKKEFENLKVIDVSDSEYLL 700

Query: 664 KTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTF-----CWECDQ 723
           +TP+ S V  +E LVL  C  L  IH S+  L+ L FLDL +CS+LK+F     C   ++
Sbjct: 701 ETPNFSKVPKLERLVLCNCRRLNHIHPSINSLHLLKFLDLTECSSLKSFSSNLRCKNLER 760

Query: 724 ------------KIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPS 783
                       +IE  ME L +LYLDGT I+ LH SIGRL GL+ L+LR C  LSSLP 
Sbjct: 761 LVLSKSGVTSSPEIEENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIMLSSLP- 820

Query: 784 NVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLR 843
           N IG  +SL++L+L GC  LD +  S  N   L+ L++ ETS+  +PP+IHS  NLK L+
Sbjct: 821 NEIGNLESLQSLLLGGCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNLKGLK 880

Query: 844 CQGLFNSIWHSLNSPS-------------FGLLSLTSLDLSNCNLVDENIPQDLH 855
             GL   IWHSL  PS             FG+ SL  L L  CNLV+E+IP+DLH
Sbjct: 881 FDGLSGRIWHSLLPPSNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLH 908

BLAST of Spg004518 vs. NCBI nr
Match: XP_038890520.1 (TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890521.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890522.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890523.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890524.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890525.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890526.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890527.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890528.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890530.1 TMV resistance protein N-like isoform X1 [Benincasa hispida])

HSP 1 Score: 701.8 bits (1810), Expect = 7.2e-198
Identity = 401/893 (44.90%), Postives = 558/893 (62.49%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDE 63
           +  YDVF+SF  +D  +T  G+L++AL+++GI  F  DK+ +     G++L    + IDE
Sbjct: 13  KMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDDKRFL----IGDDLHGLFQIIDE 72

Query: 64  SKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHF 123
           S++ IVVLS+DYAS+  CLRELTK+M+    T   RVLP+FYH+DPS V+ Q+G+F+K F
Sbjct: 73  SRSAIVVLSEDYASAKWCLRELTKIMDSMGTTMD-RVLPVFYHIDPSVVKDQSGTFKKSF 132

Query: 124 HQH------------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFS 183
            +H             +   +E+ +W+ AM+K+ N +G V+   S E +I+ K+ +Q+F 
Sbjct: 133 DEHEANALKEIDDQEKEKRLKELQSWKSAMKKIGNHTGVVITKNSSEVDIVNKIANQIFD 192

Query: 184 ALLSGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFI 243
                L   N+NLVG++     +  HL +  D+VRF+ IVGMGGIGK             
Sbjct: 193 VWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGK------------- 252

Query: 244 TTTLAEAIYHKICDEFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQ 303
            TT+A+ ++  I  +FD  CFL + G D+  ++ SLQ ++LS IF  E    F++W E+ 
Sbjct: 253 -TTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKE---DFRIWHENH 312

Query: 304 GVEEI-TRLHCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLE 363
           GVE I  RL  +KVL+V+DG E+   +  LA + +WFGPGSR+IITTRNK +L      E
Sbjct: 313 GVEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSAEWFGPGSRIIITTRNKGILNHHNYDE 372

Query: 364 IPTYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSA 423
           +  Y V  L+ D +LQLF  +AF      +  + ++  L   +++ A  LPLAL+V+GS 
Sbjct: 373 MKEYNVEELDHDSALQLFLKHAFGSN---HQNNDSFMDLSNEMIEKAKRLPLALRVIGSF 432

Query: 424 LRGRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSE 483
           L G+ + IW+E L+ L +V ++    +LK+SYD L    Q++FLDI CFF GKN DRV E
Sbjct: 433 LYGKEITIWRETLKRLIKVDERSFFHVLKISYDGLGVESQQVFLDITCFFNGKNEDRVIE 492

Query: 484 LLGSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWL 543
           +L SFG+     E++LLM RCLI++ +K+I++HDLI +MGREIVR+ES TQ  KQSRIWL
Sbjct: 493 ILESFGYSP-KSEIQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWL 552

Query: 544 RDDMKRVSVQQYGVEETRGIVLDLEEGSE--LEMEAMCISKMTKLRILEINHVRLNQQLE 603
            +D+     +++ +   +GIVL LE+  E  +E++A   S+MTKLRILEIN+V L++ +E
Sbjct: 553 HEDLYCKFAEKHDLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELSEDIE 612

Query: 604 YLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSK 663
           YLS LL  +NWPG PSK LPP FQ   L EL +P SHIL+LWDG K+F  LK+ID+S+S+
Sbjct: 613 YLSQLLRIINWPGYPSKSLPPMFQSRYLFELLLPHSHILRLWDGKKRFPKLKVIDVSNSE 672

Query: 664 NLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTF-----C-- 723
           +L  TPD SGV N+E LVL  C  L EIH S+  L++L  LDL+ C +LK F     C  
Sbjct: 673 HLRVTPDFSGVPNLERLVLSNCVKLCEIHPSINSLSKLILLDLEGCGDLKHFPPNIRCKN 732

Query: 724 ----------WECDQKIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSS 783
                      E   +I G MEHL  L+LD + I  LH SIG LTGLVLL+L  C  LSS
Sbjct: 733 LQTLKLSGTGLETSPEIGGNMEHLTHLHLDESNITQLHPSIGHLTGLVLLDLSSCLGLSS 792

Query: 784 LPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPS-IHSNNNL 843
           LP   IG  KSL+TL+L  C  LD I  S  NA  LE L +SETS+T +P S IH   NL
Sbjct: 793 LPCE-IGNLKSLKTLLLKYCEKLDQIPKSLANAESLETLCISETSITHLPSSIIHCLKNL 852

Query: 844 KVLRCQGLFNSIWHSL-------NSPSFGLLSLTSLDLSNCNLVDENIPQDLH 855
           + L C+GL   IW S+        + S GL  L +L+L  C L+DE+IP+DLH
Sbjct: 853 ETLECEGLSCGIWKSMLPQFNIHQTVSTGLGCLKTLNLMGCKLMDEDIPEDLH 878

BLAST of Spg004518 vs. NCBI nr
Match: KAA0039330.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 699.9 bits (1805), Expect = 2.7e-197
Identity = 406/892 (45.52%), Postives = 559/892 (62.67%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDE 63
           +  YDVF+SF  +D  +T  G+L++AL+++GI  F  +K+ +     G++L    K IDE
Sbjct: 13  KMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFL----IGDDLHDLFKIIDE 72

Query: 64  SKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHF 123
           S++ IVVLS+DYAS+  CLRELTK+M+    T   RVLP+FYH+DPS V+ Q+G+F+  F
Sbjct: 73  SRSAIVVLSEDYASAKWCLRELTKIMDSMG-TSMERVLPVFYHIDPSIVKDQSGTFKTSF 132

Query: 124 HQH------------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFS 183
            +H             +   +E+  W++A++K+ N +G V+   S E +I+ K+  Q+F 
Sbjct: 133 DEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNSSEVDIVNKIASQIFD 192

Query: 184 ALLSGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFI 243
           A    L   N+NLVG++     +  HL +  D+VRF+ IVGMGGIGK             
Sbjct: 193 AWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGK------------- 252

Query: 244 TTTLAEAIYHKICDEFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQ 303
            TT+A+ ++  I  +FD  CFL + G D+  ++ SLQ ++LS IF  E    F++W E+ 
Sbjct: 253 -TTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKE---DFRIWHENH 312

Query: 304 GVEEI-TRLHCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLE 363
           GVE I  RL  +KVL+V+DG E+   +  LA + DWFGPGSR+IITTRNK LL      E
Sbjct: 313 GVEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYDE 372

Query: 364 IPTYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSA 423
           +  Y V  L+ D +LQLF  +AF      +    ++  L   IV+ A  LPLAL+V+GS+
Sbjct: 373 MKEYNVEELDHDSALQLFLKHAFGSN---HQNKDSFMDLSNEIVEKAKRLPLALRVIGSS 432

Query: 424 LRGRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSE 483
           L G+ + IW+E L+ L +V ++   DILK+SYD L    Q++FLDI CFF GKN DRV+E
Sbjct: 433 LYGKEITIWRETLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNE 492

Query: 484 LLGSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWL 543
           +L SFG+     E++LLM RCLI++ +K+I++HDLI +MGREIVR+ES TQ  KQSRIWL
Sbjct: 493 ILESFGYSP-NSELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWL 552

Query: 544 RDDMKRVSVQQYGVEETRGIVLDLEEGSE--LEMEAMCISKMTKLRILEINHVRLNQQLE 603
            +D+     +++ +   +GIVL LE+  E  +E++A   S+MTKLRILEIN+V L++ +E
Sbjct: 553 HEDLYCRFAEKHDLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIE 612

Query: 604 YLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSK 663
           YLS LL  +NW G PSK LPPTFQ   L EL +P SH+L++WDG K+F  LKLID+S+S+
Sbjct: 613 YLSPLLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSNSE 672

Query: 664 NLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTFCWECDQK- 723
           +L  TPD SGV N+E LVL  C  L EIH S+  LN+L  LDL+ C +LK F      K 
Sbjct: 673 HLRVTPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKN 732

Query: 724 ----------IE-----GKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSL 783
                     +E     G MEHL  L+LDG+ I +LH SIG LTGLV L+L  C  LSSL
Sbjct: 733 LQTLKLSGTGLEIFPEIGHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSL 792

Query: 784 PSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPS-IHSNNNLK 843
           P   IG  KSL+TL+L  C  LD I  S  NA  LE L +SETS+T VP S IH   NL+
Sbjct: 793 PFE-IGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLE 852

Query: 844 VLRCQGLFNSIWHSL-------NSPSFGLLSLTSLDLSNCNLVDENIPQDLH 855
            L C+GL   IW SL        + + GL  L +L+L  C L+DE+IP+DLH
Sbjct: 853 TLDCEGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLH 877

BLAST of Spg004518 vs. NCBI nr
Match: XP_011656060.2 (TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656062.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_031741444.1 TMV resistance protein N isoform X1 [Cucumis sativus] >KAE8648981.1 hypothetical protein Csa_009304 [Cucumis sativus])

HSP 1 Score: 696.8 bits (1797), Expect = 2.3e-196
Identity = 404/893 (45.24%), Postives = 558/893 (62.49%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDE 63
           +  YDVF+SF  +D  +T  G+L++AL+++GI  F  +K+ +     G++L    K IDE
Sbjct: 13  KMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFL----IGDDLHDLFKIIDE 72

Query: 64  SKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHF 123
           S++ IVVLS+DYAS+  CLRELTK+M+    T   RVLP+FYH+DPS V+ Q+G+F+  F
Sbjct: 73  SRSAIVVLSEDYASAKWCLRELTKIMDSMG-TSMERVLPVFYHIDPSIVKDQSGTFKTSF 132

Query: 124 HQH------------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFS 183
            +H             +   +E+  W+ A++K+ N +G V+   S E +I+ K+  Q+F 
Sbjct: 133 DEHEANVLKEIDNQEKEKRLKELQNWKSALKKIGNHTGVVITKNSSEVDIVNKIASQIFD 192

Query: 184 ALLSGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFI 243
           A    L   N+NLVG++     +  HL +  D+VRF+ IVGMGGIGK             
Sbjct: 193 AWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGK------------- 252

Query: 244 TTTLAEAIYHKICDEFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQ 303
            TT+A+ ++  I  +F+  CFL + G D+  ++ SLQ ++LS IF  E    F++W E+ 
Sbjct: 253 -TTIAQVVFDCILSKFEDCCFLTLPGGDSKQSLVSLQREMLSQIFHKE---DFRIWHENH 312

Query: 304 GVEEI-TRLHCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLE 363
           GVE I  RL  +KVL+V+DG E+   +  LA + +WFGPGSR+IITTRNK LL      E
Sbjct: 313 GVEMIKNRLSGRKVLIVLDGIEERRQLEMLAGSIEWFGPGSRIIITTRNKGLLCHPNYDE 372

Query: 364 IPTYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSA 423
           +  Y V  L+ D +LQLF  +AF      +  + ++  L   IV+ A  LPLAL+V+GS+
Sbjct: 373 MKVYNVEELDHDSALQLFLKHAFGSN---HQNNDSFMDLSNEIVEKAKRLPLALRVIGSS 432

Query: 424 LRGRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSE 483
           L G+ + +W+E L+ L +V ++   D+LK+SYD L    Q++FLDI CFF GKN DRV E
Sbjct: 433 LYGKDITVWRETLKRLIKVDERNFFDVLKISYDGLGVESQQVFLDITCFFNGKNEDRVIE 492

Query: 484 LLGSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWL 543
           +L SFG+     EV+LLM RCLI++ +K+I++HDLI +MGREIVR+ES TQ  KQSRIWL
Sbjct: 493 ILESFGYSP-NSEVQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQAEKQSRIWL 552

Query: 544 RDDMKRVSVQQYGVEETRGIVLDLEEGSE--LEMEAMCISKMTKLRILEINHVRLNQQLE 603
            +D+     +++ +   +GIVL L +  E  +E++A   S+MTKLRILEI++V L++ +E
Sbjct: 553 HEDLYCRFAEKHDLMHIQGIVLSLAKEMEESIELDAESFSEMTKLRILEISNVELDEDIE 612

Query: 604 YLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSK 663
           YLS LL  +NW G PSK LPPTFQ   L EL +P SH+L++WDG K+F  LKLID+S+S+
Sbjct: 613 YLSPLLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRIWDGKKRFPKLKLIDVSNSE 672

Query: 664 NLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTFCWECDQK- 723
           +L  TPD SGV N+E LVL  C  L EIH S+  LN+L  LDL+ C +LK F      K 
Sbjct: 673 HLRVTPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKN 732

Query: 724 ----------IE-----GKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSL 783
                     +E     G MEHL  L+LDG+ I + H SIG LTGLV L+L  C  LSSL
Sbjct: 733 LQTLKLSGTGLEIFPEIGHMEHLTHLHLDGSNITHFHPSIGYLTGLVFLDLSSCLGLSSL 792

Query: 784 PSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPS-IHSNNNLK 843
           P   IG  KSL+TL+L  C  LD I  S  NA  LE L +SETS+T VPPS IH   NLK
Sbjct: 793 PCE-IGNLKSLKTLLLKYCKKLDKIPPSLANAESLETLSISETSITHVPPSIIHCLKNLK 852

Query: 844 VLRCQGLFNSIWHSLNSPSF--------GLLSLTSLDLSNCNLVDENIPQDLH 855
            L C+GL + IW SL  P F        GL  L +L+L  C L+DE+IP+DLH
Sbjct: 853 TLDCEGLSHGIWKSL-LPQFNINQTITTGLGCLKALNLMGCKLMDEDIPEDLH 877

BLAST of Spg004518 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.1e-127
Identity = 312/879 (35.49%), Postives = 477/879 (54.27%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           RW YDVF+SF  +DT  T T HL+E L+  GI  F+ DK+L        EL  KAI+ES+
Sbjct: 9   RWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGEL-CKAIEESQ 68

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
             IVV S++YA+S  CL EL K+ME ++  K   V+P+FY VDPSHVR+Q  SF K F +
Sbjct: 69  FAIVVFSENYATSRWCLNELVKIMECKTRFKQ-TVIPIFYDVDPSHVRNQKESFAKAFEE 128

Query: 124 HT---KTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALLSGLTTSNE 183
           H    K   E +  WR A+ + ANL GS       +A+ I+++  Q+ S L     +  +
Sbjct: 129 HETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKISLSYLQ 188

Query: 184 NLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYHK 243
           N+VGI    +++   L++  + VR +GI GMGG+GK              TT+A AI+  
Sbjct: 189 NIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGK--------------TTIARAIFDT 248

Query: 244 ICD------EFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGVEEI 303
           +        +FDG+CFL+    +    + SLQN LLS + + + ++     +ED   +  
Sbjct: 249 LLGRMDSSYQFDGACFLK-DIKENKRGMHSLQNALLSELLREKANYNN---EEDGKHQMA 308

Query: 304 TRLHCKKVLLVVDGAE-KDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYE 363
           +RL  KKVL+V+D  + KD ++  LA   DWFG GSR+IITTR+K L+    K +I  YE
Sbjct: 309 SRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLI---EKNDI-IYE 368

Query: 364 VHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRP 423
           V  L + +S+QLF  +AF  E    + + N+  L   +V +A GLPLALKV GS L    
Sbjct: 369 VTALPDHESIQLFKQHAFGKE----VPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLR 428

Query: 424 LGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSF 483
           L  W+  +E +   S   I+D LK+SYD LE +QQE+FLDIACF +G+  D + ++L S 
Sbjct: 429 LTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESC 488

Query: 484 GHQQVMQEVELLMNRCLIDLLN-KRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDM 543
            H      + +L+++ L+ +    ++ MHDLIQDMG+ IV  +     G++SR+WL  ++
Sbjct: 489 -HIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQKDP--GERSRLWLAKEV 548

Query: 544 KRVSVQQYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLSNLL 603
           + V     G      I +     S L      +  M +LR+  +     +  ++YL N L
Sbjct: 549 EEVMSNNTGTMAMEAIWVS-SYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNL 608

Query: 604 GFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLLKTP 663
                   P +  P TF+   L+ L++  + +  LW  TK   SL+ IDLS SK L +TP
Sbjct: 609 RCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTP 668

Query: 664 DLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTF------------CW 723
           D +G+ N+E + L  C++L E+H S+   +++  L L DC +LK F              
Sbjct: 669 DFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFPCVNVESLEYLGLR 728

Query: 724 ECDQ-----KIEGKMEHLEKLYLDGTAIRNLHSSIGRL-TGLVLLNLRDCKRLSSLPSNV 783
            CD      +I G+M+   ++++ G+ IR L SSI +  T +  L L + K L +LPS++
Sbjct: 729 SCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSI 788

Query: 784 IGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLRCQ 843
             + KSL +L + GCS L+ +     + + L   D S+T + + P SI   N L +L  +
Sbjct: 789 C-RLKSLVSLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLNKLIILMFR 848

Query: 844 GLFNSIWHSLNSPSFGLLSLTSLDLSNCNLVDENIPQDL 854
           G  + +       + GL SL  L+LS CNL+D  +P+++
Sbjct: 849 GFKDGVHFEFPPVAEGLHSLEYLNLSYCNLIDGGLPEEI 854

BLAST of Spg004518 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.9e-124
Identity = 314/878 (35.76%), Postives = 466/878 (53.08%), Query Frame = 0

Query: 7   YDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESKTCI 66
           YDVF+SF  +DT  T  GHLF AL + GI  F  DK+L        EL +KAI ES+  +
Sbjct: 12  YDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSEL-MKAIGESRFAV 71

Query: 67  VVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFE---KHFHQ 126
           VV SK+YASST CL EL K++E   E   L V+P+FY VDPS VR Q G +      F  
Sbjct: 72  VVFSKNYASSTWCLEELVKILEIH-EKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEA 131

Query: 127 HTKTHAEEVLTWRDAMEKVANLSGSVVYPT--SDEAEIIKKMTHQVFSALLSGLTTSNEN 186
           +     ++VL WR+A+ KVAN+SG  +  T   DE++ I+++   +F      ++ +N +
Sbjct: 132 NLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRD 191

Query: 187 LVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYHKI 246
           LVGI    K++   L M    VR +GI GMGG+GK              TT A A++++ 
Sbjct: 192 LVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGK--------------TTAARALFNRY 251

Query: 247 CDEFDGSCFLR-VSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGVEEITRLHCK 306
              F+ +CFL  V     ++ +  LQ  LLS + K E  F      E+  V    RL  K
Sbjct: 252 YQNFESACFLEDVKEYLQHHTLLYLQKTLLSKLLKVE--FVDCTDTEEMCVILKRRLCSK 311

Query: 307 KVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPLEED 366
           KVL+V+D    +  + KL    DWFG GSR++ITTR+  LL +    E  TYE+  LE+D
Sbjct: 312 KVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHDVHE--TYEIKVLEKD 371

Query: 367 DSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIWQEI 426
           ++++LF  +AF         +  +  L   +V +  GLPLALKVLGS L    L +W   
Sbjct: 372 EAIELFNLHAFKRSS----PEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWIST 431

Query: 427 LESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSFGHQQVMQ 486
           ++ L    + +I+  LK+S+D L   ++ IFLDIACFF+G N   ++ L  + G   V+ 
Sbjct: 432 IDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVL- 491

Query: 487 EVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRVSVQQY 546
            V+ L+ + LI +L  +I MHDL+Q+MGR+I  +ES  +     RI+  +D+K   +   
Sbjct: 492 GVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQESPMR-----RIYRPEDVKDACIGDM 551

Query: 547 GVEETRGIVL----DLEEGS-ELEMEAMCISKMTKLRIL--EINHVRLNQQLEYLSNLLG 606
             E   G++L      EEG  E    A  + K  +LRIL  E  +   ++ + YL N L 
Sbjct: 552 RKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLL 611

Query: 607 FLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLLKTPD 666
           +L W    S   P  F+P+ L+ L M  S I++LW+G K+   L  +DLS    L++TPD
Sbjct: 612 WLEWRNYSSNSFPSNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPD 671

Query: 667 LSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLK----------------T 726
              + N+E L+L  C +L E+H SV  L  L  L++  C +L+                 
Sbjct: 672 FRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLN 731

Query: 727 FCWECDQ--KIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPSNVI 786
           +C+      ++E  M HL+KL L  T IR L +SI  L+ L  L +  C +L SLPS+ I
Sbjct: 732 YCFNLKMFPEVERNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSS-I 791

Query: 787 GKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSI---HSNNNLKVLR 846
            +F++L+   +  C  L  +     N+NC  EL L   S+ ++P SI    S N L++  
Sbjct: 792 WRFRNLK---ISECEKLGSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICN 849

Query: 847 CQGLFNSIWHSLNSPSFGLLSLTSLDLSNCNLVDENIP 851
           C+ +      SL+S  +GL SLT+L L +C  + +N+P
Sbjct: 852 CKTI-----SSLSSSIWGLTSLTTLKLLDCRKL-KNLP 849

BLAST of Spg004518 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 3.5e-123
Identity = 324/895 (36.20%), Postives = 462/895 (51.62%), Query Frame = 0

Query: 7   YDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE---LKAIDESK 66
           YDVF+SF  +DT    T HL+ AL + GI  F+ DK       +GE +    LKAI+ES+
Sbjct: 27  YDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRDDK-----LRRGEAIAPELLKAIEESR 86

Query: 67  TCIVVLSKDYASSTKCLRELTKVMERRSETKT--LRVLPLFYHVDPSHVRHQTGSFEKHF 126
           + ++V S++YA S  CL EL K+ME   + K     V P+FYHVDPSHVR Q GSF + F
Sbjct: 87  SSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHVRKQEGSFGEAF 146

Query: 127 HQHTKTHAEEVLTWRDAMEKVANLSGSVVYPTSD--EAEIIKKMTHQVFSALLSGLTTSN 186
             + +   +++  WR A+ + ANLSG   +P  D  E+  IK++T  +F  L      + 
Sbjct: 147 AGYGENLKDKIPRWRTALTEAANLSG---WPLQDGYESNQIKEITDSIFRRLKCKRLDAG 206

Query: 187 ENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYH 246
            NLVGI    KE+   L M+S +VR +G+ G+GGIGK              TT+A+ IY+
Sbjct: 207 ANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGK--------------TTIAKVIYN 266

Query: 247 KICDEFDGSCFL-RVSGSDTNNNITSLQNQLLSTIFKNEKSFQF-QVWDEDQGVEEITRL 306
           ++  EF+   FL  +        ++ LQNQLL  I K E S     V      +++I  L
Sbjct: 267 ELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDI--L 326

Query: 307 HCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPL 366
             K V +V+D  +    +  L    +W G GSRVIITTRNK  +LD  K++   YEV  L
Sbjct: 327 SSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKH-VLDVQKVD-DLYEVKGL 386

Query: 367 EEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIW 426
             +++ +LF  YA  FEQ+  L  ++Y  L   +V +  GLPLALKVLG  L  + +  W
Sbjct: 387 NFEEACELFSLYA--FEQN--LPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEW 446

Query: 427 QEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSFG-HQ 486
           +  L  L +  + +I+ +LK SYD L   ++ IFLD+ACFFKG++ D VS++L +   H 
Sbjct: 447 ESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHA 506

Query: 487 QVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRVS 546
           ++   ++ L ++CLI L   RI MHDLIQ MG EIVR +   +  K SR+W   D +R  
Sbjct: 507 EI--GIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERAL 566

Query: 547 VQQYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEI-------------------- 606
               G++    I LDL +   +   +   +KMT+LR+L++                    
Sbjct: 567 TAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVE 626

Query: 607 ---------NHVRLNQQLEYLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQL 666
                    + +RL +  ++ S  L +L W G P   LP  F    L+EL + CS+I QL
Sbjct: 627 LYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQL 686

Query: 667 WDGTKKFGSLKLIDLSDSKNLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFL 726
             G K    LK+IDLS S+ L +  + S + N+E L LRGC SL +IH SV  + +L  L
Sbjct: 687 RLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTL 746

Query: 727 DLKDCSNLK-----------------TFCWECDQKIE--GKMEHLEKLYLDGTAIRNLHS 786
            LK C  LK                  +C + ++  E  G M+ L +L L  TAI++L  
Sbjct: 747 SLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDLPD 806

Query: 787 SIGRLTGLVLLNLRDCKRLSSLPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEEL 844
           SIG L  L  L+L DC +    P    G  KSLR L L   ++ DL + S  +   LE L
Sbjct: 807 SIGDLESLKYLDLSDCSKFEKFPEKG-GNMKSLRELDLRNTAIKDLPD-SIRDLESLERL 866

BLAST of Spg004518 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.7e-115
Identity = 300/878 (34.17%), Postives = 436/878 (49.66%), Query Frame = 0

Query: 3   RRWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE---LKAI 62
           R   YDVF+SF  +DT    T HL+ AL + GI  F+ D+       +GE +    LKAI
Sbjct: 20  RTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRDDR-----LRRGEAIAPELLKAI 79

Query: 63  DESKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEK 122
           +ES++ ++V S++YA S  CL EL K+ME + +     V P+FYHVDPSHVR Q GSF +
Sbjct: 80  EESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGH-AVFPIFYHVDPSHVRKQEGSFGE 139

Query: 123 HFHQHTKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALLSGLTTSN 182
            F  + +   +++  WR A+ + ANLSG  +     E+  IK++T+ +F  L        
Sbjct: 140 AFAGYEENWKDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLKCKRLDVG 199

Query: 183 ENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYH 242
            NLVGI    KE+   L ++S +VR +GI G+GGIGK              TT+A+ IY+
Sbjct: 200 ANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGIGK--------------TTIAKVIYN 259

Query: 243 KICDEFDGSCFLRVSGSDTNNNIT-SLQNQLLSTIFKNEKSFQF-QVWDEDQGVEEITRL 302
           ++  EF+   FL      +N  +   LQNQLL  I + E S     V      +++I  L
Sbjct: 260 ELSCEFEYMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDI--L 319

Query: 303 HCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPL 362
             ++V +V+D  +    +  L    +W G GSRVIITTRNK +L      ++  YEV  L
Sbjct: 320 LSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQEVDDL--YEVEGL 379

Query: 363 EEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIW 422
             +++ +LF  YAF       L  ++Y  L   +V +  GLPLALKVLGS L  + +  W
Sbjct: 380 NFEEACELFSLYAFK----QNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQW 439

Query: 423 QEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSFGHQQ 482
           +  L+ L       I  +LK SYD L+   + IFLD+ACFFKG+  D V  +L       
Sbjct: 440 EGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDF-P 499

Query: 483 VMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRVSV 542
               +  L + CLI L   +I MHDLIQ MG EIVR     +  K SR+W   D +R   
Sbjct: 500 AETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALT 559

Query: 543 QQYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEI--------------------- 602
              G++    + LDL +   +   +   +KMTKLR+L++                     
Sbjct: 560 ADEGIKSVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVY 619

Query: 603 -------NHVRLNQQLEYLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWD 662
                  + ++L Q  ++ S  L +L W G P   LP  F    L+EL + CS+I QLW 
Sbjct: 620 DVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQ 679

Query: 663 GTKKFGSLKLIDLSDSKNLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDL 722
           G K    LK+IDLS S+ L +  + S + N+E L L GC SL +IH SV  + +L  L L
Sbjct: 680 GHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSL 739

Query: 723 KDCSNLKTFCWECDQKIEGKMEHLEKLYLDG-TAIRNLHSSIGRLTGLVLLNLRDCKRLS 782
           + C+ LK           G +E LE LYL   +         G +  L  L+L++   + 
Sbjct: 740 RSCNKLKNL-----PDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKN-TAIK 799

Query: 783 SLPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNL 842
            LP + IG  +SL +L L  CS  +       N   L ELDL  T++  +P SI    +L
Sbjct: 800 DLPDS-IGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESL 859

Query: 843 KVLRCQGLFNSIWHSLNSPSFGLLSLTSLDLSNCNLVD 847
           ++L       + +         + SL  LDL N  + D
Sbjct: 860 EILNLSDC--AKFEKFPEKGGNMKSLKELDLQNTAIKD 859

BLAST of Spg004518 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.0e-114
Identity = 302/890 (33.93%), Postives = 467/890 (52.47%), Query Frame = 0

Query: 7   YDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDESKT 66
           +DVF+SF   DT N  TGHL +AL   GI  F  D+       +G+ L      I++SK 
Sbjct: 11  FDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDDR-----LRRGDNLTALFDRIEKSKI 70

Query: 67  CIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQH 126
            I+V S +YA+S  CLREL K++E R+  + L V+P+FY VD S V  Q  SF   F   
Sbjct: 71  AIIVFSTNYANSAWCLRELVKILECRNSNQQL-VVPIFYKVDKSDVEKQRNSFAVPFKLP 130

Query: 127 TKTH----AEEVLTWRDAMEKVANLSGSVVYPTS-DEAEIIKKMTHQVFSALLSGLTTSN 186
             T      EE+ +W+ A+   +N+ G VV   S  EA+++ ++    F  L     + N
Sbjct: 131 ELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAPSGN 190

Query: 187 ENLVGISRSFKEVYEHLDMKS-DEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIY 246
           E LVGI    K + + L  +  D V  +GIVGM GIGK              TTLA+ +Y
Sbjct: 191 EGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGK--------------TTLADCLY 250

Query: 247 HKICDEFDGSCFL-RVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGVEEITRL 306
            ++  +FDGSCFL  +  +   + + SL  +L ST+  N++  +           E  RL
Sbjct: 251 GRMRGQFDGSCFLTNIRENSGRSGLESLLQKLFSTVL-NDRDLEIGAPGNAHERFE-RRL 310

Query: 307 HCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPL 366
             K++L+V+D    +  I  L     W+  GSR+IITTR+  L+     ++   Y +  L
Sbjct: 311 KSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI---ETIKGRKYVLPKL 370

Query: 367 EEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIW 426
            + ++L+LF   AFS    + L +  +  L   ++ +A G PLALKVLGS L  R    W
Sbjct: 371 NDREALKLFSLNAFS--NSFPLKE--FEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYW 430

Query: 427 QEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSFGHQQ 486
           +  L+ L   S   I ++L+ SY+ L   Q+ +FLDIACFF+ +N+D V+ LL S G   
Sbjct: 431 EAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHG-VD 490

Query: 487 VMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESS---------TQFGKQS---- 546
           V   V+ L+++CLI L + RI MHD++Q M +EI  +  +         ++ G Q     
Sbjct: 491 VSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHI 550

Query: 547 RIWLRDDMKRVSVQQYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEI--NH---- 606
           R+W  +D+  +  +  G ++ RGI LD  +   + + A     M  L+ L+I  +H    
Sbjct: 551 RLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRG 610

Query: 607 ------VRLNQQLEYLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTK 666
                 + L + L +L N L +L+W G P + +P  F P NL++L++P S + ++WD  K
Sbjct: 611 CEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEK 670

Query: 667 KFGSLKLIDLSDSKNLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDC 726
             G LK +DLS S NL +   L+   N+E L L GCTSL+++ S++  L +L +L+L+DC
Sbjct: 671 DVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDC 730

Query: 727 SNLKTF----------------CWECDQKIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGL 786
           ++L++                 C    +K     E++E L LDGT I++L  SI     L
Sbjct: 731 TSLRSLPKGIKTQSLQTLILSGCSSL-KKFPLISENVEVLLLDGTVIKSLPESIQTFRRL 790

Query: 787 VLLNLRDCKRLSSLPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLT 845
            LLNL++CK+L  L S++  K K L+ LIL GCS L++     ++   LE L + +TS+T
Sbjct: 791 ALLNLKNCKKLKHLSSDLY-KLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSIT 850

BLAST of Spg004518 vs. ExPASy TrEMBL
Match: A0A6J1CL15 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012122 PE=4 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 1.5e-217
Identity = 436/894 (48.77%), Postives = 573/894 (64.09%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           R  YDVF+SF  +DT N   G L+EALHQ+GI+ F  D+KL+   +  E L +KAI+ES+
Sbjct: 56  RMTYDVFISFRGEDTRNNFVGFLYEALHQLGIMTFMDDRKLLIGDDLSENL-MKAIEESR 115

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
           + IVVLSKDYASS  CL+EL K+M+  SET T +VLP+FYHVDPSHVRHQ+G+F+K F +
Sbjct: 116 SSIVVLSKDYASSKWCLKELVKIMDCMSET-THQVLPVFYHVDPSHVRHQSGNFKKSFDE 175

Query: 124 H-----------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALL 183
           H            + +  EV  W+D+M K+ NL+G VV   S E  I+KK+T+Q+F    
Sbjct: 176 HEEDAKKITGQQKEKYLVEVKNWKDSMTKIGNLTGEVVTQHSSEVNIVKKITNQIFERWR 235

Query: 184 SGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTT 243
             + TS++NLVG++    ++   L +  D+VRF+GIVGMGGIGK              TT
Sbjct: 236 PKIATSDKNLVGMTYQLLKMNVKLGLGLDDVRFVGIVGMGGIGK--------------TT 295

Query: 244 LAEAIYHKICDEFDGSCFLRV-SGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGV 303
           +A+ +Y  I   F+GSCFLRV  GS   NN+ SLQ QLLS +F  E     ++WDED G 
Sbjct: 296 IAKVVYDCIKSNFEGSCFLRVFGGSSKQNNLLSLQEQLLSRLFLKE---NVRMWDEDYGA 355

Query: 304 EEITRLHC-KKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIP 363
           E I    C +KVLLV+DG E+   +  LA +PDWFGPGSR+IITTRNK++L      EI 
Sbjct: 356 EMIKNQLCSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEIL--HQPYEII 415

Query: 364 TYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALR 423
            Y++  L+ D +LQLFC  AF  +      D+N+  L + I++    LPLALKV+GS L 
Sbjct: 416 EYKLKLLDADSALQLFCNRAFGID----YIDSNFMNLSKEILEKLGRLPLALKVIGSYLN 475

Query: 424 GRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELL 483
            +   IW+E L  L +V +K + ++LK+SYD L    Q++FLD+AC F G+  D+V E+L
Sbjct: 476 KKEEVIWKETLYRLIKVDEKNLFEVLKISYDGLGVESQKVFLDLACLFHGRKKDKVIEIL 535

Query: 484 GSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRD 543
             FG+     E++L M RCL+++   +I+MHDL+  +G+EIV RES TQ GKQSRIW ++
Sbjct: 536 ECFGYSP-YSELQLFMERCLLEVSPTKILMHDLVVALGQEIVHRESLTQPGKQSRIWFQE 595

Query: 544 DMKRVSVQQYGVEETRGIVLDLE-EGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLS 603
           D+ RV   ++G++ T GIVL+LE +  EL +EA   + MT LRILEI++V+L+  + +LS
Sbjct: 596 DVDRVFTVKHGLKYTEGIVLNLEKKQKELILEAKSFTDMTYLRILEISNVQLSGNINFLS 655

Query: 604 NLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLL 663
           NLLGF+NWPG PSKCLPP FQ   L+EL +P S I  LW+G K F  LK+ID+SDSK LL
Sbjct: 656 NLLGFINWPGYPSKCLPPNFQSRCLLELHLPLSSIEILWEGKKDFEKLKVIDVSDSKYLL 715

Query: 664 KTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTF-----CWECD- 723
            TPD S V N+E LVLR C  L  IH S+  LNRL  LDL +CS+ K+F     C   + 
Sbjct: 716 GTPDFSKVPNLERLVLRNCRKLYHIHPSINDLNRLRLLDLSNCSSFKSFSSHLRCKSLEG 775

Query: 724 -----------QKIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPS 783
                       K E  MEHL +L+L GT I+ LHSSIG L GLVLL+LR+C  LSSLPS
Sbjct: 776 LILSESGLTSSPKFEVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIMLSSLPS 835

Query: 784 NVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLR 843
             IG  KSL+ L+L GC  LD I  S  NA CLE LD+ +TS+ Q PP+IH   NLK L 
Sbjct: 836 E-IGNLKSLQVLLLEGCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENLKRLN 895

Query: 844 CQGLFNSIWHSL------------NSPSFGLLSLTSLDLSNCNLVDENIPQDLH 855
           C+GL   IWHSL            +   FGL SL  L L NCNLVDE+IP+DLH
Sbjct: 896 CEGLSRKIWHSLLPRDIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLH 922

BLAST of Spg004518 vs. ExPASy TrEMBL
Match: A0A6J1CJ31 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 2.8e-208
Identity = 419/895 (46.82%), Postives = 575/895 (64.25%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           R  YDVF+SF  +DT N   G L++AL Q+GI+ F  DKKL+   +  E+  +KAI+ES+
Sbjct: 41  RMTYDVFISFRGEDTRNNFVGFLYQALRQLGIMTFMDDKKLLIGDDLSEKF-VKAIEESR 100

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
           + IVVLSKDYASS  CL+EL K+M+  SET T RVLP+FYHVDPSHVRHQ+G+F+K F  
Sbjct: 101 SSIVVLSKDYASSKWCLKELAKIMDCMSET-THRVLPVFYHVDPSHVRHQSGNFKKSFDG 160

Query: 124 H-----------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALL 183
           H            + + +EV  W+D+M K+ NL+G VV   S E +++KK+T+Q+F    
Sbjct: 161 HEEKAPKLIGVQREEYLKEVQNWKDSMTKIGNLAGEVVTQHSSEVDVVKKITNQIFERWR 220

Query: 184 SGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTT 243
             + TS++NLVG++R   E+   L +  D+VRF+GIVGMGGIGK              TT
Sbjct: 221 PKIATSDKNLVGMTRQLLEMNVKLGLGLDDVRFVGIVGMGGIGK--------------TT 280

Query: 244 LAEAIYHKICDEFDGSCFLRV-SGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGV 303
           +A+ +Y  I  +F+GSCFLRV  GS    N+ SLQ QLLS +F  E     ++WDED G 
Sbjct: 281 IAKVVYDCITSKFEGSCFLRVFGGSSKQCNLVSLQEQLLSRLFLKE---NVRIWDEDYGA 340

Query: 304 EEITRLHC-KKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIP 363
           E I    C +KVLLV+D  E+   +  LA +PDWFGPGSR+IITTRNK++L      EI 
Sbjct: 341 EMIENQLCSRKVLLVLDEVEEKEQLEMLARSPDWFGPGSRIIITTRNKEIL--HQPYEIL 400

Query: 364 TYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALR 423
            Y++  L++D +LQLFC  AF  +      D+++  L + +V+    LPLALKV+GS L 
Sbjct: 401 EYKMKLLDDDSALQLFCNRAFGID----YIDSSFMNLSKGMVEKLGRLPLALKVIGSYLN 460

Query: 424 GRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELL 483
            + + IW+E L  L +V +K + ++L +SYD L A  Q++FLD+ACFF G+  D+V ++L
Sbjct: 461 KKDVLIWKETLNRLIKVDEKHLFEVLNISYDGLGAESQKVFLDLACFFNGRRTDKVIKIL 520

Query: 484 GSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRD 543
            SFG+     E+ L   +CLI++ + +I+MHDLI  +G+EIVR+ES TQ GKQSRIWL++
Sbjct: 521 ESFGYNP-HSELNLFTEKCLIEVSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQE 580

Query: 544 DMKRVSVQQYGVEETRGIVLDLE-EGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLS 603
           D+ R    ++G++ T GIVL+LE +  EL +EA   + MT LRILEI++V+L   ++ LS
Sbjct: 581 DVVRAFTMKHGLKYTEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLYGNIKSLS 640

Query: 604 NLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLL 663
           NLL  LNWPG PSKCLP  FQ   L+EL +  S I+++WDG K+F +LK+ID+SDS+ LL
Sbjct: 641 NLLALLNWPGYPSKCLPSNFQSGYLLELHLRHSSIVRIWDGKKEFENLKVIDVSDSEYLL 700

Query: 664 KTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTF-----CWECDQ 723
           +TP+ S V  +E LVL  C  L  IH S+  L+ L FLDL +CS+LK+F     C   ++
Sbjct: 701 ETPNFSKVPKLERLVLCNCRRLNHIHPSINSLHLLKFLDLTECSSLKSFSSNLRCKNLER 760

Query: 724 ------------KIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPS 783
                       +IE  ME L +LYLDGT I+ LH SIGRL GL+ L+LR C  LSSLP 
Sbjct: 761 LVLSKSGVTSSPEIEENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIMLSSLP- 820

Query: 784 NVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLR 843
           N IG  +SL++L+L GC  LD +  S  N   L+ L++ ETS+  +PP+IHS  NLK L+
Sbjct: 821 NEIGNLESLQSLLLGGCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNLKGLK 880

Query: 844 CQGLFNSIWHSLNSPS-------------FGLLSLTSLDLSNCNLVDENIPQDLH 855
             GL   IWHSL  PS             FG+ SL  L L  CNLV+E+IP+DLH
Sbjct: 881 FDGLSGRIWHSLLPPSNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLH 908

BLAST of Spg004518 vs. ExPASy TrEMBL
Match: A0A5A7T7V5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001220 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 406/892 (45.52%), Postives = 559/892 (62.67%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDE 63
           +  YDVF+SF  +D  +T  G+L++AL+++GI  F  +K+ +     G++L    K IDE
Sbjct: 13  KMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFL----IGDDLHDLFKIIDE 72

Query: 64  SKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHF 123
           S++ IVVLS+DYAS+  CLRELTK+M+    T   RVLP+FYH+DPS V+ Q+G+F+  F
Sbjct: 73  SRSAIVVLSEDYASAKWCLRELTKIMDSMG-TSMERVLPVFYHIDPSIVKDQSGTFKTSF 132

Query: 124 HQH------------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFS 183
            +H             +   +E+  W++A++K+ N +G V+   S E +I+ K+  Q+F 
Sbjct: 133 DEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNSSEVDIVNKIASQIFD 192

Query: 184 ALLSGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFI 243
           A    L   N+NLVG++     +  HL +  D+VRF+ IVGMGGIGK             
Sbjct: 193 AWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGK------------- 252

Query: 244 TTTLAEAIYHKICDEFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQ 303
            TT+A+ ++  I  +FD  CFL + G D+  ++ SLQ ++LS IF  E    F++W E+ 
Sbjct: 253 -TTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKE---DFRIWHENH 312

Query: 304 GVEEI-TRLHCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLE 363
           GVE I  RL  +KVL+V+DG E+   +  LA + DWFGPGSR+IITTRNK LL      E
Sbjct: 313 GVEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYDE 372

Query: 364 IPTYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSA 423
           +  Y V  L+ D +LQLF  +AF      +    ++  L   IV+ A  LPLAL+V+GS+
Sbjct: 373 MKEYNVEELDHDSALQLFLKHAFGSN---HQNKDSFMDLSNEIVEKAKRLPLALRVIGSS 432

Query: 424 LRGRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSE 483
           L G+ + IW+E L+ L +V ++   DILK+SYD L    Q++FLDI CFF GKN DRV+E
Sbjct: 433 LYGKEITIWRETLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNE 492

Query: 484 LLGSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWL 543
           +L SFG+     E++LLM RCLI++ +K+I++HDLI +MGREIVR+ES TQ  KQSRIWL
Sbjct: 493 ILESFGYSP-NSELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWL 552

Query: 544 RDDMKRVSVQQYGVEETRGIVLDLEEGSE--LEMEAMCISKMTKLRILEINHVRLNQQLE 603
            +D+     +++ +   +GIVL LE+  E  +E++A   S+MTKLRILEIN+V L++ +E
Sbjct: 553 HEDLYCRFAEKHDLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIE 612

Query: 604 YLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSK 663
           YLS LL  +NW G PSK LPPTFQ   L EL +P SH+L++WDG K+F  LKLID+S+S+
Sbjct: 613 YLSPLLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSNSE 672

Query: 664 NLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTFCWECDQK- 723
           +L  TPD SGV N+E LVL  C  L EIH S+  LN+L  LDL+ C +LK F      K 
Sbjct: 673 HLRVTPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKN 732

Query: 724 ----------IE-----GKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSL 783
                     +E     G MEHL  L+LDG+ I +LH SIG LTGLV L+L  C  LSSL
Sbjct: 733 LQTLKLSGTGLEIFPEIGHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSL 792

Query: 784 PSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPS-IHSNNNLK 843
           P   IG  KSL+TL+L  C  LD I  S  NA  LE L +SETS+T VP S IH   NL+
Sbjct: 793 PFE-IGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLE 852

Query: 844 VLRCQGLFNSIWHSL-------NSPSFGLLSLTSLDLSNCNLVDENIPQDLH 855
            L C+GL   IW SL        + + GL  L +L+L  C L+DE+IP+DLH
Sbjct: 853 TLDCEGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLH 877

BLAST of Spg004518 vs. ExPASy TrEMBL
Match: A0A0A0KXU0 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647530 PE=4 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-196
Identity = 404/893 (45.24%), Postives = 558/893 (62.49%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDE 63
           +  YDVF+SF  +D  +T  G+L++AL+++GI  F  +K+ +     G++L    K IDE
Sbjct: 80  KMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFL----IGDDLHDLFKIIDE 139

Query: 64  SKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHF 123
           S++ IVVLS+DYAS+  CLRELTK+M+    T   RVLP+FYH+DPS V+ Q+G+F+  F
Sbjct: 140 SRSAIVVLSEDYASAKWCLRELTKIMDSMG-TSMERVLPVFYHIDPSIVKDQSGTFKTSF 199

Query: 124 HQH------------TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFS 183
            +H             +   +E+  W+ A++K+ N +G V+   S E +I+ K+  Q+F 
Sbjct: 200 DEHEANVLKEIDNQEKEKRLKELQNWKSALKKIGNHTGVVITKNSSEVDIVNKIASQIFD 259

Query: 184 ALLSGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFI 243
           A    L   N+NLVG++     +  HL +  D+VRF+ IVGMGGIGK             
Sbjct: 260 AWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGK------------- 319

Query: 244 TTTLAEAIYHKICDEFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQ 303
            TT+A+ ++  I  +F+  CFL + G D+  ++ SLQ ++LS IF  E    F++W E+ 
Sbjct: 320 -TTIAQVVFDCILSKFEDCCFLTLPGGDSKQSLVSLQREMLSQIFHKE---DFRIWHENH 379

Query: 304 GVEEI-TRLHCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLE 363
           GVE I  RL  +KVL+V+DG E+   +  LA + +WFGPGSR+IITTRNK LL      E
Sbjct: 380 GVEMIKNRLSGRKVLIVLDGIEERRQLEMLAGSIEWFGPGSRIIITTRNKGLLCHPNYDE 439

Query: 364 IPTYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSA 423
           +  Y V  L+ D +LQLF  +AF      +  + ++  L   IV+ A  LPLAL+V+GS+
Sbjct: 440 MKVYNVEELDHDSALQLFLKHAFGSN---HQNNDSFMDLSNEIVEKAKRLPLALRVIGSS 499

Query: 424 LRGRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSE 483
           L G+ + +W+E L+ L +V ++   D+LK+SYD L    Q++FLDI CFF GKN DRV E
Sbjct: 500 LYGKDITVWRETLKRLIKVDERNFFDVLKISYDGLGVESQQVFLDITCFFNGKNEDRVIE 559

Query: 484 LLGSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWL 543
           +L SFG+     EV+LLM RCLI++ +K+I++HDLI +MGREIVR+ES TQ  KQSRIWL
Sbjct: 560 ILESFGYSP-NSEVQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQAEKQSRIWL 619

Query: 544 RDDMKRVSVQQYGVEETRGIVLDLEEGSE--LEMEAMCISKMTKLRILEINHVRLNQQLE 603
            +D+     +++ +   +GIVL L +  E  +E++A   S+MTKLRILEI++V L++ +E
Sbjct: 620 HEDLYCRFAEKHDLMHIQGIVLSLAKEMEESIELDAESFSEMTKLRILEISNVELDEDIE 679

Query: 604 YLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSK 663
           YLS LL  +NW G PSK LPPTFQ   L EL +P SH+L++WDG K+F  LKLID+S+S+
Sbjct: 680 YLSPLLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRIWDGKKRFPKLKLIDVSNSE 739

Query: 664 NLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTFCWECDQK- 723
           +L  TPD SGV N+E LVL  C  L EIH S+  LN+L  LDL+ C +LK F      K 
Sbjct: 740 HLRVTPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKN 799

Query: 724 ----------IE-----GKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSL 783
                     +E     G MEHL  L+LDG+ I + H SIG LTGLV L+L  C  LSSL
Sbjct: 800 LQTLKLSGTGLEIFPEIGHMEHLTHLHLDGSNITHFHPSIGYLTGLVFLDLSSCLGLSSL 859

Query: 784 PSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPS-IHSNNNLK 843
           P   IG  KSL+TL+L  C  LD I  S  NA  LE L +SETS+T VPPS IH   NLK
Sbjct: 860 PCE-IGNLKSLKTLLLKYCKKLDKIPPSLANAESLETLSISETSITHVPPSIIHCLKNLK 919

Query: 844 VLRCQGLFNSIWHSLNSPSF--------GLLSLTSLDLSNCNLVDENIPQDLH 855
            L C+GL + IW SL  P F        GL  L +L+L  C L+DE+IP+DLH
Sbjct: 920 TLDCEGLSHGIWKSL-LPQFNINQTITTGLGCLKALNLMGCKLMDEDIPEDLH 944

BLAST of Spg004518 vs. ExPASy TrEMBL
Match: M4QSV5 (Resistance gene-like protein OS=Cucumis melo OX=3656 GN=RGH11 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 1.6e-195
Identity = 407/894 (45.53%), Postives = 559/894 (62.53%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDE 63
           +  YDVF+SF  +D  +T  G+L++AL+++GI  F  +K+ +     G++L    K IDE
Sbjct: 13  KMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFL----IGDDLHDLFKIIDE 72

Query: 64  SKTCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHF 123
           S++ IVVLS+DYAS+  CLRELTK+M+    T   RVLP+FYH+DPS V+ Q+G+F+  F
Sbjct: 73  SRSAIVVLSEDYASAKWCLRELTKIMDSMG-TSMERVLPVFYHIDPSIVKDQSGTFKTSF 132

Query: 124 HQH------------TKTHAEEVLTWRDAMEKVANLSGSVVYPT-SDEAEIIKKMTHQVF 183
            +H             +   +E+  W++A++K+ N +G V+    S E +I+ K+  Q+F
Sbjct: 133 DEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNRSSEVDIVNKIASQIF 192

Query: 184 SALLSGLTTSNENLVGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFF 243
            A    L   N+NLVG++     +  HL +  D+VRF+ IVGMGGIGK            
Sbjct: 193 DAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIGK------------ 252

Query: 244 ITTTLAEAIYHKICDEFDGSCFLRVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDED 303
             TT+A+ ++  I  +FD  CFL + G D+  ++ SLQ ++LS IF  E    F++W E+
Sbjct: 253 --TTIAQVVFDCILSKFDDCCFLTLPGGDSKQSLVSLQREMLSQIFHKE---DFRIWHEN 312

Query: 304 QGVEEI-TRLHCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKL 363
            GVE I  RL  +KVL+V+DG E+   +  LA + DWFGPGSR+IITTRNK LL      
Sbjct: 313 HGVEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYD 372

Query: 364 EIPTYEVHPLEEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGS 423
           E+  Y V  L+ D +LQLF  +AF      +    ++  L   IV+ A  LPLAL+V+GS
Sbjct: 373 EMKEYNVEELDHDSALQLFLKHAFGSN---HQNKDSFMDLSNEIVEKAKRLPLALRVIGS 432

Query: 424 ALRGRPLGIWQEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVS 483
           +L G+ + IW+E L+ L +V ++   DILK+SYD L    Q++FLDI CFF GKN DRV+
Sbjct: 433 SLYGKEITIWRETLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVN 492

Query: 484 ELLGSFGHQQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIW 543
           E+L SFG+     E++LLM RCLI++ +K+I++HDLI +MGREIVR+ES TQ  KQSRIW
Sbjct: 493 EILESFGYSP-NSELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIW 552

Query: 544 LRDDMK-RVSVQQYGVEETRGIVLDLEEGSE--LEMEAMCISKMTKLRILEINHVRLNQQ 603
           L +D+  R + +   +   +GIVL LE+  E  +E++A   S+MTKLRILEIN+V L++ 
Sbjct: 553 LHEDLYCRFAEKHVDLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDED 612

Query: 604 LEYLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSD 663
           +EYLS LL  +NW G PSK LPPTFQ   L EL +P SH+L++WDG K+F  LKLID+S+
Sbjct: 613 IEYLSPLLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSN 672

Query: 664 SKNLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTFCWECDQ 723
           S++L  TPD SGV N+E LVL  C  L EIH S+  LN+L  LDL+ C +LK F      
Sbjct: 673 SEHLRVTPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRC 732

Query: 724 K-----------IE-----GKMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLS 783
           K           +E     G MEHL  L+LDG+ I +LH SIG LTGLV L+L  C  LS
Sbjct: 733 KNLQTLKLSGTGLEIFPEIGHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLS 792

Query: 784 SLPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPS-IHSNNN 843
           SLP   IG  KSL+TL+L  C  LD I  S  NA  LE L +SETS+T VP S IH   N
Sbjct: 793 SLPFE-IGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKN 852

Query: 844 LKVLRCQGLFNSIWHSL-------NSPSFGLLSLTSLDLSNCNLVDENIPQDLH 855
           L+ L C+GL   IW SL        + + GL  L +L+L  C L+DE+IP+DLH
Sbjct: 853 LETLDCEGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLH 879

BLAST of Spg004518 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 416.0 bits (1068), Expect = 7.3e-116
Identity = 302/890 (33.93%), Postives = 467/890 (52.47%), Query Frame = 0

Query: 7   YDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELE--LKAIDESKT 66
           +DVF+SF   DT N  TGHL +AL   GI  F  D+       +G+ L      I++SK 
Sbjct: 11  FDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDDR-----LRRGDNLTALFDRIEKSKI 70

Query: 67  CIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQH 126
            I+V S +YA+S  CLREL K++E R+  + L V+P+FY VD S V  Q  SF   F   
Sbjct: 71  AIIVFSTNYANSAWCLRELVKILECRNSNQQL-VVPIFYKVDKSDVEKQRNSFAVPFKLP 130

Query: 127 TKTH----AEEVLTWRDAMEKVANLSGSVVYPTS-DEAEIIKKMTHQVFSALLSGLTTSN 186
             T      EE+ +W+ A+   +N+ G VV   S  EA+++ ++    F  L     + N
Sbjct: 131 ELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAPSGN 190

Query: 187 ENLVGISRSFKEVYEHLDMKS-DEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIY 246
           E LVGI    K + + L  +  D V  +GIVGM GIGK              TTLA+ +Y
Sbjct: 191 EGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGK--------------TTLADCLY 250

Query: 247 HKICDEFDGSCFL-RVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGVEEITRL 306
            ++  +FDGSCFL  +  +   + + SL  +L ST+  N++  +           E  RL
Sbjct: 251 GRMRGQFDGSCFLTNIRENSGRSGLESLLQKLFSTVL-NDRDLEIGAPGNAHERFE-RRL 310

Query: 307 HCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPL 366
             K++L+V+D    +  I  L     W+  GSR+IITTR+  L+     ++   Y +  L
Sbjct: 311 KSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI---ETIKGRKYVLPKL 370

Query: 367 EEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIW 426
            + ++L+LF   AFS    + L +  +  L   ++ +A G PLALKVLGS L  R    W
Sbjct: 371 NDREALKLFSLNAFS--NSFPLKE--FEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYW 430

Query: 427 QEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSFGHQQ 486
           +  L+ L   S   I ++L+ SY+ L   Q+ +FLDIACFF+ +N+D V+ LL S G   
Sbjct: 431 EAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHG-VD 490

Query: 487 VMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESS---------TQFGKQS---- 546
           V   V+ L+++CLI L + RI MHD++Q M +EI  +  +         ++ G Q     
Sbjct: 491 VSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHI 550

Query: 547 RIWLRDDMKRVSVQQYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEI--NH---- 606
           R+W  +D+  +  +  G ++ RGI LD  +   + + A     M  L+ L+I  +H    
Sbjct: 551 RLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRG 610

Query: 607 ------VRLNQQLEYLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTK 666
                 + L + L +L N L +L+W G P + +P  F P NL++L++P S + ++WD  K
Sbjct: 611 CEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEK 670

Query: 667 KFGSLKLIDLSDSKNLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDC 726
             G LK +DLS S NL +   L+   N+E L L GCTSL+++ S++  L +L +L+L+DC
Sbjct: 671 DVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDC 730

Query: 727 SNLKTF----------------CWECDQKIEGKMEHLEKLYLDGTAIRNLHSSIGRLTGL 786
           ++L++                 C    +K     E++E L LDGT I++L  SI     L
Sbjct: 731 TSLRSLPKGIKTQSLQTLILSGCSSL-KKFPLISENVEVLLLDGTVIKSLPESIQTFRRL 790

Query: 787 VLLNLRDCKRLSSLPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLT 845
            LLNL++CK+L  L S++  K K L+ LIL GCS L++     ++   LE L + +TS+T
Sbjct: 791 ALLNLKNCKKLKHLSSDLY-KLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSIT 850

BLAST of Spg004518 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 403.7 bits (1036), Expect = 3.8e-112
Identity = 294/895 (32.85%), Postives = 452/895 (50.50%), Query Frame = 0

Query: 5   WRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESKT 64
           W+ DVFVSF  +D   T   HLF    +MGI  F+ D  L        EL + AI  S+ 
Sbjct: 16  WKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPEL-IDAIKGSRF 75

Query: 65  CIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQH 124
            IVV+S++YA+S+ CL EL K+ME   +T    ++P+FY VDPS VR Q GSF +    H
Sbjct: 76  AIVVVSRNYAASSWCLDELLKIMECNKDT----IVPIFYEVDPSDVRRQRGSFGEDVESH 135

Query: 125 TKTHAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALLSGLTTSNENLVG 184
             +  E+V  W++A++K+A +SG       D++++IKK+   +   L+S     ++ L+G
Sbjct: 136 --SDKEKVGKWKEALKKLAAISGE-DSRNWDDSKLIKKIVKDISDKLVSTSWDDSKGLIG 195

Query: 185 ISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYHKICDE 244
           +S     +   + +   +VR LGI GMGG+GK              TT+A+ +Y+++  +
Sbjct: 196 MSSHMDFLQSMISIVDKDVRMLGIWGMGGVGK--------------TTIAKYLYNQLSGQ 255

Query: 245 FDGSCFL-RVSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGVEEI-TRLHCKKV 304
           F   CF+  V        +  LQ + L  +F+       + W        I  R   K V
Sbjct: 256 FQVHCFMENVKEVCNRYGVRRLQVEFLCRMFQERDK---EAWSSVSCCNIIKERFRHKMV 315

Query: 305 LLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPLEEDDS 364
            +V+D  ++   + +L     WFGPGSR+I+TTR++ LLL      +  Y+V  L + ++
Sbjct: 316 FIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLV--YKVKCLPKKEA 375

Query: 365 LQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIWQEILE 424
           LQLFC YAF  E    +    +  L    V +ASGLPLAL+VLGS L  R    W+  L 
Sbjct: 376 LQLFCNYAFREE---IILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLA 435

Query: 425 SLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGSFGHQQVMQEV 484
            L       I+++L+VSYD L+ +++ IFL I+CF+  K +D V +LL   G+   +  +
Sbjct: 436 RLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEI-GI 495

Query: 485 ELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRVSVQQYGV 544
            +L  + LI   N  + +HDL++ MGRE+VR+++     ++  +W  +D+  +  +  G 
Sbjct: 496 TILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGT 555

Query: 545 EETRGIVLDLEEGSELEMEAMCISKMTKLRILEI--------NHVRLNQQLEYLSNLLGF 604
           +   GI L+L E SE+         ++ L++L            V L   L YL   L +
Sbjct: 556 QLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRY 615

Query: 605 LNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLSDSKNLLKTPDL 664
           L W G P K +P  F P  L+EL M  S++ +LWDG +   +LK +DLS  K L++ PDL
Sbjct: 616 LRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDL 675

Query: 665 SGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCSNLKTFCWECDQK-IE------ 724
           S   N+E L L  C SL E+  S+  L  L+   L +C  LK        K +E      
Sbjct: 676 SKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSG 735

Query: 725 -GKMEHL-------EKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPSNVIGKFK 784
              ++H         +LYL  T I  L SSI RL+ LV L++ DC+RL +LPS  +G   
Sbjct: 736 CSSLKHFPEISWNTRRLYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPS-YLGHLV 795

Query: 785 SLRTLILLGCSLLDLIEFSSDNANCLEELD---------------------LSETSLTQV 844
           SL++L L GC  L+ +  +  N   LE L+                     +SETS+ ++
Sbjct: 796 SLKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISETSIEEI 855

Query: 845 PPSIHSNNNLKVLRCQGLFNSIWHSLNSPSFGLLSLTSLDLSNCNLVDENIPQDL 854
           P  I + + L+ L      N    SL      L SL  L LS C+++ E+ P ++
Sbjct: 856 PARICNLSQLRSLDISE--NKRLASLPVSISELRSLEKLKLSGCSVL-ESFPLEI 875

BLAST of Spg004518 vs. TAIR 10
Match: AT4G19510.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 387.9 bits (995), Expect = 2.1e-107
Identity = 289/842 (34.32%), Postives = 436/842 (51.78%), Query Frame = 0

Query: 7   YDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELEL--KAIDESKT 66
           +DVFVSF   DT +  T HL + L   GI  F  D KL      GE + L    I++SK 
Sbjct: 17  FDVFVSFRGADTRHDFTSHLVKYLRGKGIDVFS-DAKL----RGGEYISLLFDRIEQSKM 76

Query: 67  CIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQH 126
            IVV S+DYA+S  CL E+ K+M+RR E     VLP+FY V  S V +QTGSFE  F   
Sbjct: 77  SIVVFSEDYANSWWCLEEVGKIMQRRKEFNH-GVLPIFYKVSKSDVSNQTGSFEAVFQSP 136

Query: 127 TKT---HAEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALLSGLT--TSN 186
           TK      +++   + A++  +N+ G V    S E + + ++    F  +L+ L+     
Sbjct: 137 TKIFNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTF-RMLNELSPCVIP 196

Query: 187 ENLVGISRSFKEVYEHLDMKSDE-VRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIY 246
           ++L GI    KE+ + L   +DE VR +G++GM GIGK              TT+A+ +Y
Sbjct: 197 DDLPGIESRSKELEKLLMFDNDECVRVVGVLGMTGIGK--------------TTVADIVY 256

Query: 247 HKICDEFDGSCFLR-VSGSDTNNNITSLQNQLLSTIFKNEKSFQFQVWDEDQGVEEITRL 306
            +    FDG  FL  +  +     +  L  +LL  +   E      V    QG  E   L
Sbjct: 257 KQNFQRFDGYEFLEDIEDNSKRYGLPYLYQKLLHKLLDGE-----NVDVRAQGRPE-NFL 316

Query: 307 HCKKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPL 366
             KK+ +V+D   ++  I  L    + +  GSR++I TR+K LL    K    TY V  L
Sbjct: 317 RNKKLFIVLDNVTEEKQIEYLIGKKNVYRQGSRIVIITRDKKLL---QKNADATYVVPRL 376

Query: 367 EEDDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIW 426
            + ++++LFC   F    HY      +  L    V +A GLPLALK+LG  L    +  W
Sbjct: 377 NDREAMELFCLQVFG--NHY--PTEEFVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYW 436

Query: 427 QEILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELLGS--FGH 486
           ++ LE L    DK++   LK SY AL+  Q+ +FLDIACFF+ +  D VS +L S     
Sbjct: 437 KKKLEFLQVNPDKELQKELKSSYKALDDDQKSVFLDIACFFRSEKADFVSSILKSDDIDA 496

Query: 487 QQVMQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRV 546
           + VM+E+E    +CL+ +   RI MHDL+  MG+EI + +S  + G++ R+W   D++ +
Sbjct: 497 KDVMRELE---EKCLVTISYDRIEMHDLLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDI 556

Query: 547 SVQQYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEINHVRLNQQL---------- 606
                G E  RGI L++ E   +++     + ++KL+ L+ +    +Q            
Sbjct: 557 LEHNTGTECVRGIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHSSHCSQWCDNDHIFQCSK 616

Query: 607 --EYLSNLLGFLNWPGCPSKCLPPTFQPANLIELRMPCSHILQLWDGTKKFGSLKLIDLS 666
             ++  + L +L+W G P  CLP  F P  L++L +  SHI QLW+  K   SL+ +DL 
Sbjct: 617 VPDHFPDELVYLHWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLG 676

Query: 667 DSKNLLKTPDLSGVLNIESLVLRGCTSLREIHSSVTRLNRLAFLDLKDCS---------- 726
            SK+LL    LS   N+E L L GCTSL ++  SV ++N L +L+L+DC+          
Sbjct: 677 QSKDLLNLSGLSRAKNLERLDLEGCTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFK 736

Query: 727 --NLKTFCWECDQKIEG---KMEHLEKLYLDGTAIRNLHSSIGRLTGLVLLNLRDCKRLS 786
             +LKT       K++      E +E L+L+GTAI  +   I  L  L+LLNL++C++L 
Sbjct: 737 IKSLKTLILSGCLKLKDFHIISESIESLHLEGTAIERVVEHIESLHSLILLNLKNCEKLK 796

Query: 787 SLPSNVIGKFKSLRTLILLGCSLLDLIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNL 811
            LP N + K KSL+ L+L GCS L+ +    +   CLE L +  TS+ Q  P +   +NL
Sbjct: 797 YLP-NDLYKLKSLQELVLSGCSALESLPPIKEKMECLEILLMDGTSIKQT-PEMSCLSNL 818

BLAST of Spg004518 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 382.9 bits (982), Expect = 6.9e-106
Identity = 294/860 (34.19%), Postives = 425/860 (49.42%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           RW YDVFVSF   D       HL+++L + GI  F  D +L        EL L AI+ SK
Sbjct: 11  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPEL-LNAIETSK 70

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
             IVVL+KDYASS  CL EL  +M+      +  V P+F +VDPS +R Q GS+ K F +
Sbjct: 71  ILIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSK 130

Query: 124 HTKTH-AEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALLSGLTTSNENL 183
           H  +H   ++  WR+A+ KVAN+SG  +    +EAE I  +T ++   L           
Sbjct: 131 HKNSHPLNKLKDWREALTKVANISGWDI-KNRNEAECIADITREILKRLPCQYLHVPSYA 190

Query: 184 VGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYHKIC 243
           VG+    + +   L + SD VR + I GMGGIGK              TTLA+  +++  
Sbjct: 191 VGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGK--------------TTLAKVAFNEFS 250

Query: 244 DEFDGSCFLR--VSGSDTNNNITSLQNQLLSTIF-KNEKSFQFQVWDEDQGVEEITRLHC 303
             F+GS FL      S      T LQ+QLLS I  +N+  F+      D  V+E  R   
Sbjct: 251 HLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFK----GLDHAVKE--RFRS 310

Query: 304 KKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPLEE 363
           K+VLLVVD  +    +   A   D FG GSR+IITTRN  LL    + E  +Y    L+ 
Sbjct: 311 KRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQL-RAE-GSYSPKELDG 370

Query: 364 DDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIWQE 423
           D+SL+LF  +AF   +        +    E +V + +GLPLA++VLG+ L  R +  W+ 
Sbjct: 371 DESLELFSWHAFRTSE----PPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWES 430

Query: 424 ILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELL-GSFGHQQV 483
            L+ L ++ +  I   L++S++AL   Q+++FLDIACFF G +   V+ +L G   +  +
Sbjct: 431 TLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDI 490

Query: 484 MQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRVSVQ 543
           +  + LLM RCLI +    IMMHDL++DMGR+IVR  S  + G++SR+W  +D+  V  +
Sbjct: 491 V--LSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKK 550

Query: 544 QYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLSNLLGFLNWP 603
           + G     G+ L  +       E    +KM +LR+LE+ +V LN   E+    L +L W 
Sbjct: 551 KSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWH 610

Query: 604 GCPSKCLPPTFQPANLIELRMPCSHILQLWDG---TKKFGSLKLIDLSDSKNLLKTPDLS 663
           G   +C P      +L  L +  S++ + W      +    +K +DLS S  L +TPD S
Sbjct: 611 GFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFS 670

Query: 664 GVLNIESLVLRGCTSLREIHSSVTRLN-RLAFLDLKDCSNLKTFCWECDQKIEGKMEHLE 723
              N+E L+L  C SL  +H S+  L+ +L  L+L  C  L     E       K++ LE
Sbjct: 671 YFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEI-----YKLKSLE 730

Query: 724 KLYLDG-TAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPSNVIGKFKSLRTLILLGCSLLD 783
            L+L   + +  L  ++G L  L  L L D   L  +PS  I + K L+ L L GC  L 
Sbjct: 731 SLFLSNCSKLERLDDALGELESLTTL-LADFTALREIPS-TINQLKKLKRLSLNGCKGL- 790

Query: 784 LIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLRCQGLFNSIWHSLNSPSFGLLS 843
               S D  N   E   S + L  V  S                            GL  
Sbjct: 791 ---LSDDIDNLYSEKSHSVSLLRPVSLS----------------------------GLTY 801

Query: 844 LTSLDLSNCNLVDENIPQDL 854
           +  L L  CNL DE IP+D+
Sbjct: 851 MRILSLGYCNLSDELIPEDI 801

BLAST of Spg004518 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 382.9 bits (982), Expect = 6.9e-106
Identity = 294/860 (34.19%), Postives = 425/860 (49.42%), Query Frame = 0

Query: 4   RWRYDVFVSFLEKDTGNTITGHLFEALHQMGIVGFKPDKKLVEDYEKGEELELKAIDESK 63
           RW YDVFVSF   D       HL+++L + GI  F  D +L        EL L AI+ SK
Sbjct: 14  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPEL-LNAIETSK 73

Query: 64  TCIVVLSKDYASSTKCLRELTKVMERRSETKTLRVLPLFYHVDPSHVRHQTGSFEKHFHQ 123
             IVVL+KDYASS  CL EL  +M+      +  V P+F +VDPS +R Q GS+ K F +
Sbjct: 74  ILIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSK 133

Query: 124 HTKTH-AEEVLTWRDAMEKVANLSGSVVYPTSDEAEIIKKMTHQVFSALLSGLTTSNENL 183
           H  +H   ++  WR+A+ KVAN+SG  +    +EAE I  +T ++   L           
Sbjct: 134 HKNSHPLNKLKDWREALTKVANISGWDI-KNRNEAECIADITREILKRLPCQYLHVPSYA 193

Query: 184 VGISRSFKEVYEHLDMKSDEVRFLGIVGMGGIGKTTDRSGEGRYFFITTTLAEAIYHKIC 243
           VG+    + +   L + SD VR + I GMGGIGK              TTLA+  +++  
Sbjct: 194 VGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGK--------------TTLAKVAFNEFS 253

Query: 244 DEFDGSCFLR--VSGSDTNNNITSLQNQLLSTIF-KNEKSFQFQVWDEDQGVEEITRLHC 303
             F+GS FL      S      T LQ+QLLS I  +N+  F+      D  V+E  R   
Sbjct: 254 HLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFK----GLDHAVKE--RFRS 313

Query: 304 KKVLLVVDGAEKDFHITKLAATPDWFGPGSRVIITTRNKDLLLDFPKLEIPTYEVHPLEE 363
           K+VLLVVD  +    +   A   D FG GSR+IITTRN  LL    + E  +Y    L+ 
Sbjct: 314 KRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQL-RAE-GSYSPKELDG 373

Query: 364 DDSLQLFCTYAFSFEQHYYLADANYSTLCESIVKHASGLPLALKVLGSALRGRPLGIWQE 423
           D+SL+LF  +AF   +        +    E +V + +GLPLA++VLG+ L  R +  W+ 
Sbjct: 374 DESLELFSWHAFRTSE----PPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWES 433

Query: 424 ILESLGQVSDKKIVDILKVSYDALEARQQEIFLDIACFFKGKNIDRVSELL-GSFGHQQV 483
            L+ L ++ +  I   L++S++AL   Q+++FLDIACFF G +   V+ +L G   +  +
Sbjct: 434 TLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDI 493

Query: 484 MQEVELLMNRCLIDLLNKRIMMHDLIQDMGREIVRRESSTQFGKQSRIWLRDDMKRVSVQ 543
           +  + LLM RCLI +    IMMHDL++DMGR+IVR  S  + G++SR+W  +D+  V  +
Sbjct: 494 V--LSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKK 553

Query: 544 QYGVEETRGIVLDLEEGSELEMEAMCISKMTKLRILEINHVRLNQQLEYLSNLLGFLNWP 603
           + G     G+ L  +       E    +KM +LR+LE+ +V LN   E+    L +L W 
Sbjct: 554 KSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWH 613

Query: 604 GCPSKCLPPTFQPANLIELRMPCSHILQLWDG---TKKFGSLKLIDLSDSKNLLKTPDLS 663
           G   +C P      +L  L +  S++ + W      +    +K +DLS S  L +TPD S
Sbjct: 614 GFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFS 673

Query: 664 GVLNIESLVLRGCTSLREIHSSVTRLN-RLAFLDLKDCSNLKTFCWECDQKIEGKMEHLE 723
              N+E L+L  C SL  +H S+  L+ +L  L+L  C  L     E       K++ LE
Sbjct: 674 YFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEI-----YKLKSLE 733

Query: 724 KLYLDG-TAIRNLHSSIGRLTGLVLLNLRDCKRLSSLPSNVIGKFKSLRTLILLGCSLLD 783
            L+L   + +  L  ++G L  L  L L D   L  +PS  I + K L+ L L GC  L 
Sbjct: 734 SLFLSNCSKLERLDDALGELESLTTL-LADFTALREIPS-TINQLKKLKRLSLNGCKGL- 793

Query: 784 LIEFSSDNANCLEELDLSETSLTQVPPSIHSNNNLKVLRCQGLFNSIWHSLNSPSFGLLS 843
               S D  N   E   S + L  V  S                            GL  
Sbjct: 794 ---LSDDIDNLYSEKSHSVSLLRPVSLS----------------------------GLTY 804

Query: 844 LTSLDLSNCNLVDENIPQDL 854
           +  L L  CNL DE IP+D+
Sbjct: 854 MRILSLGYCNLSDELIPEDI 804