Spg004486 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg004486 vs. NCBI nr
Match: XP_038889504.1 (putative endo-1,3(4)-beta-glucanase 2 [Benincasa hispida])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 589/693 (84.99%), Postives = 631/693 (91.05%), Query Frame = 0

Query: 1   METPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPL 60
           METPKPWLI IFFF      + L+  PP  + QF FP+  STAVPDP+KFFSPTLLSSPL
Sbjct: 23  METPKPWLISIFFF----FFIILTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPL 82

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALN 120
           PTNSFFQNFVLNNGD PEYIHPY++RTANSS SVSYPSR SNSSITQL F+PDLTI+AL+
Sbjct: 83  PTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALD 142

Query: 121 RTSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVD 180
           RTSPVSN THF+SSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLK SSVSISTGNGVRSVD
Sbjct: 143 RTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVD 202

Query: 181 SYDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEK 240
           SYDDSTK+ IRL+NGR+WVLYSSSAIYL+KSK NQIVTSGGFSGVIR+AVLP+S +ES  
Sbjct: 203 SYDDSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGN 262

Query: 241 ILDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRYG 300
           ILD+Y GCYPVSGY KLS NFGFKYKWQK+GSGGLLMLAH LHR++L  NQTVL NLRYG
Sbjct: 263 ILDRYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYG 322

Query: 301 SIDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAA 360
           SIDGDLLGVVGDSWDLNF+P+PITWHSINGIDKKFFPEIVAALKRD  TLNVTELSSTA+
Sbjct: 323 SIDGDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTAS 382

Query: 361 SYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAG 420
           SYFY KLLARA+RLALIAEEVD  AGVIPAVV+FLKNGIQPWL GKF KNGFLYERKWAG
Sbjct: 383 SYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAG 442

Query: 421 LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMN 480
           LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVL KLDPNWG ++K QAYALV DYMN
Sbjct: 443 LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMN 502

Query: 481 FRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEG 540
           FRPKKSQFSIPFRNFDFWK HSWAAGLT+FPDGRNQESTSEAVNAYYAAALMG+AY DE 
Sbjct: 503 FRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDES 562

Query: 541 LSAAGAVLTAAEIAATQTWWHVK-EENSFYDEGFAEENRMVGILWSAARESRLWFAPPEW 600
           L+AAG+ LTAAEIAATQTWWHVK E+N  YD+GF EENR+VGILWSAARESRLWFAP EW
Sbjct: 563 LTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEW 622

Query: 601 KECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKK 660
           +ECRVGIQV PV+PV+ERVFSD GF KEVVEWVS ALER DAGEGWKGFAYALE +YDKK
Sbjct: 623 RECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKK 682

Query: 661 SAVGKLKKLKKHDDGNSLSNLLWWIYSRP-GRG 692
           SA+ K+KKLKKHDDGNSLSNLLWWIYSRP GRG
Sbjct: 683 SAIEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 711

BLAST of Spg004486 vs. NCBI nr
Match: KAG7025154.1 (hypothetical protein SDJN02_13977, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 573/693 (82.68%), Postives = 617/693 (89.03%), Query Frame = 0

Query: 1   METPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPL 60
           ME P PWL+  F   LL    A SD  PPS+ QFPFPE  STAVPDP+KFFSP+LLSSPL
Sbjct: 1   MEAPNPWLLPFF---LLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPL 60

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALN 120
           PTNSFFQNFVLNNGDLPEYIHPYLIRTANSS SVSYPSR+SNSSITQLPF PDLTI    
Sbjct: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTI---- 120

Query: 121 RTSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GNGVRSV 180
             S VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLK SSVSIST GNGV SV
Sbjct: 121 --SSVSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKNSSVSISTGGNGVGSV 180

Query: 181 DSYDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESE 240
           DSYDDSTK+ IRLSNGR+WV+YS++AIYL+K K ++IVTSGGFSGVIRVAVLP+S  E+E
Sbjct: 181 DSYDDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETE 240

Query: 241 KILDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRY 300
           +ILD+Y GCYPVSGYAKLSGNFGF YKWQK+GSGGLLMLAH LHRR+L EN TVLQN +Y
Sbjct: 241 QILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKY 300

Query: 301 GSIDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTA 360
           GSIDGDL+GVVGDSWDLNF PIPITWHSINGI++KFFPEIVAALK D  TLNVTELSSTA
Sbjct: 301 GSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTA 360

Query: 361 ASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWA 420
           ASYFYGKLLARA+RLALIAEEVDYAAGVIPAVVKFLK G+QPWLDGKF +NGFLY+RKWA
Sbjct: 361 ASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWA 420

Query: 421 GLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYM 480
           GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVL KLD NWGN+YKA AYALV DYM
Sbjct: 421 GLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYM 480

Query: 481 NFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDE 540
           N+R K SQFSIPFRNFDFWK HSWAAGLT+FPDGRNQESTSEA+NAYYAAALMG+AYGDE
Sbjct: 481 NYRSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDE 540

Query: 541 GLSAAGAVLTAAEIAATQTWWHVKE--ENSFYDEGFAEENRMVGILWSAARESRLWFAPP 600
            L+A G+ LTAAEIAA QTWWHV     +S YDEGF EENR+VGILWS ARESRLWFA  
Sbjct: 541 SLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATA 600

Query: 601 EWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYD 660
           EW+ECRVGIQV PV+PVTERVF+D GF KEVVEWVSPALER DAGEGWKGF YALE +YD
Sbjct: 601 EWRECRVGIQVLPVLPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYD 660

Query: 661 KKSAVGKLKKLKKHDDGNSLSNLLWWIYSRPGR 691
           K++AVGK+KKLKKHDDGNSLSNLLWWIYSRP R
Sbjct: 661 KENAVGKVKKLKKHDDGNSLSNLLWWIYSRPER 684

BLAST of Spg004486 vs. NCBI nr
Match: XP_022925518.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita moschata])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 575/694 (82.85%), Postives = 618/694 (89.05%), Query Frame = 0

Query: 1   METPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPL 60
           ME P PWL+  F   LL A  A SD  PPS+ QFPFPE  STAVPDP+KFFSP+LLSSPL
Sbjct: 1   MEAPNPWLLPFFLLLLLAA--AFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPL 60

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALN 120
           PTNSFFQNFVLNNGDLPEYIHPYLIRTANSS SVSYPSR+SNSSITQLPF PDLTI    
Sbjct: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTI---- 120

Query: 121 RTSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GNGVRSV 180
             S VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSS+SIST GNGV SV
Sbjct: 121 --SSVSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSISISTGGNGVGSV 180

Query: 181 DSYDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESE 240
           DSYD STK+ IRLSNGRSWV+YS++AIYL+K K ++IVTSGGFSGVIRVAVLP+S  E+E
Sbjct: 181 DSYDGSTKHIIRLSNGRSWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETE 240

Query: 241 KILDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRY 300
           +ILD+Y GCYPVSGYAKLSGNFGF YKWQK+GSGGLLMLAH LHRR+L EN TVLQN +Y
Sbjct: 241 QILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKY 300

Query: 301 GSIDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTA 360
           GSIDGDL+GVVGDSWDLNF PIPITWHSINGI++KFFPEIVAALK D  TLNVTELSSTA
Sbjct: 301 GSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTA 360

Query: 361 ASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWA 420
           ASYFYGKLLARA+RLALIAEEVDYAAGVIPAVVKFLK G+QPWLDGKF +NGFLY+RKWA
Sbjct: 361 ASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWA 420

Query: 421 GLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYM 480
           GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVL KLD NWGN+YKA AYALV DYM
Sbjct: 421 GLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYM 480

Query: 481 NFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDE 540
           N+R K SQFSIPFRNFDFWK HSWAAGLT+FPDGRNQESTSEA+NAYYAAALMG+AYGDE
Sbjct: 481 NYRSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDE 540

Query: 541 GLSAAGAVLTAAEIAATQTWWHVKE--ENSFYDEGFAEENRMVGILWSAARESRLWFAPP 600
            L+A G+ LTAAEIAA QTWWHV     +S YDEGF EENR+VGILWS ARESRLWFA  
Sbjct: 541 SLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATA 600

Query: 601 EWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYD 660
           EW+ECRVGIQV PVVPVTERVF+D GF KEVVEWVSPALER DAGEGWKGF YALE +YD
Sbjct: 601 EWRECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYD 660

Query: 661 -KKSAVGKLKKLKKHDDGNSLSNLLWWIYSRPGR 691
            K++AVGK+KKLKKHDDGNSLSNLLWWIYSRP R
Sbjct: 661 NKENAVGKVKKLKKHDDGNSLSNLLWWIYSRPER 686

BLAST of Spg004486 vs. NCBI nr
Match: XP_023534896.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 572/693 (82.54%), Postives = 617/693 (89.03%), Query Frame = 0

Query: 1   METPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPL 60
           ME P PWL+  F   LL    A SD  PPS+ QFPFPE  STAVPDPSKFFSP+LLSSPL
Sbjct: 1   MEAPNPWLLPFF---LLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPSKFFSPSLLSSPL 60

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALN 120
           PTNSFFQNFVLNNGDLPEYIHPYLIRTANSS SVSYPSR+SNSSITQLPF PDLTI    
Sbjct: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTI---- 120

Query: 121 RTSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GNGVRSV 180
             S VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST GNGV SV
Sbjct: 121 --SSVSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGGNGVGSV 180

Query: 181 DSYDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESE 240
           DSYDDSTK+ IRLSNGR+WV+YS++AIYL+K K ++IVTSGGF GVIRVAVLP+S  E+E
Sbjct: 181 DSYDDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFFGVIRVAVLPNSAAETE 240

Query: 241 KILDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRY 300
           +ILD+Y GCYPVSGYAKLSG+FGF YKWQK+GSGGLLMLAH LHRR+L EN TVLQN +Y
Sbjct: 241 QILDRYSGCYPVSGYAKLSGSFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKY 300

Query: 301 GSIDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTA 360
           GSIDGDL+GVVGDSWDLNF PIPITWHSINGI++KFFPEIVAALK D  TLNVTELSSTA
Sbjct: 301 GSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTA 360

Query: 361 ASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWA 420
           ASYFYGKLLARA+RLALIAEEVDYAAGVIPAVVKFLK G+QPWLDGKF +NGFLY+RKWA
Sbjct: 361 ASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWA 420

Query: 421 GLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYM 480
           GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVL KLD NWG++YKA AYALV DYM
Sbjct: 421 GLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGSQYKAPAYALVYDYM 480

Query: 481 NFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDE 540
           N+R K +QFSIPFRNFDFWK HSWAAGLT+FPDGRNQESTSEA+NAYYAAALMG+AYGDE
Sbjct: 481 NYRSKNTQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDE 540

Query: 541 GLSAAGAVLTAAEIAATQTWWHVKE--ENSFYDEGFAEENRMVGILWSAARESRLWFAPP 600
            L+A G+ LTAAEIAA QTWWHV     +S YDEGF EENR+VGILWS ARESRLWFA  
Sbjct: 541 SLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATA 600

Query: 601 EWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYD 660
           EW+ECRVGIQV PVVPVTERVF+D GF KEVVEWVSPALER DAGEGWKGF YALE +YD
Sbjct: 601 EWRECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYD 660

Query: 661 KKSAVGKLKKLKKHDDGNSLSNLLWWIYSRPGR 691
           K++AVGK+KKLKKHDDGNSLSNLLWWIYSRP R
Sbjct: 661 KENAVGKVKKLKKHDDGNSLSNLLWWIYSRPER 684

BLAST of Spg004486 vs. NCBI nr
Match: KAA0039339.1 (putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo var. makuwa] >TYK00522.1 putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo var. makuwa])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 574/683 (84.04%), Postives = 611/683 (89.46%), Query Frame = 0

Query: 11  IFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPLPTNSFFQNFV 70
           +FFF LLTA         P + QFPFPE  STAVPDPSKFFSP LLSSPLPTNSFFQNFV
Sbjct: 13  LFFFLLLTA--------APVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFV 72

Query: 71  LNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALNRTSPVSNRTH 130
           LNNGD PEYIHPYLIRTANSS  VSYPSR SNSSI QL FFPDL I++LN+T   SN TH
Sbjct: 73  LNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATH 132

Query: 131 FVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDSTKYFI 190
           FVSSFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V IST NGVRSVDSY+D TKY I
Sbjct: 133 FVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYII 192

Query: 191 RLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKILDQYRGCYP 250
           RL+NGRSW+LYSSSAIYL+KSK NQ+VTSGGF GVIRVAVLPDS +ESEKILD+Y GCYP
Sbjct: 193 RLNNGRSWLLYSSSAIYLIKSKSNQVVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYP 252

Query: 251 VSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRYGSIDGDLLGVV 310
           VSG+AKLSG FGF+YKWQK+GSGGLLMLAHTLHR +LP +QTVLQNLRY SIDGDLLGVV
Sbjct: 253 VSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVV 312

Query: 311 GDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAASYFYGKLLAR 370
           GDSWDL F+PIPITWHSINGID KFFPEIVAALKRD  TLNVTELSST ASYFYGKLLAR
Sbjct: 313 GDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLAR 372

Query: 371 ASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTKNGATST 430
           A+RLALIAEEV+Y A VIPAVVKFLKNGIQPWL GKF KNGFLYERKWAGLVTKNGATST
Sbjct: 373 AARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATST 432

Query: 431 TEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNFRPKKSQFSI 490
            EDFGFGIYNDHHFHLGYFVYSIAVL KLDPNWG +YK QAYAL+ DYMNFRPKKSQFSI
Sbjct: 433 IEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSI 492

Query: 491 PFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGLSAAGAVLTA 550
           PFRNFDFWK HSWAAGL +FPDGRNQESTSEAVNAYYAAALMG+AYGD  L+AAG+ LTA
Sbjct: 493 PFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTA 552

Query: 551 AEIAATQTWWHV-KEENSFYDEGFAEENRMVGILWSAARESRLWFAPPEWKECRVGIQVF 610
           AEI A+QTWWHV KE+N  YD+GFAEENRMVGILWSAARESRLWFAP EW+ECR+GIQV 
Sbjct: 553 AEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVL 612

Query: 611 PVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKKSAVGKLKKLK 670
           PV+PVTER+F+DVGF KE VEWV PALER DAGEGWKGFAYALE +YDKKSAV K+KKLK
Sbjct: 613 PVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLK 672

Query: 671 KHDDGNSLSNLLWWIYSRP-GRG 692
           KHDDGNSLSNLLWWIYSRP GRG
Sbjct: 673 KHDDGNSLSNLLWWIYSRPEGRG 684

BLAST of Spg004486 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.7e-41
Identity = 182/659 (27.62%), Postives = 286/659 (43.40%), Query Frame = 0

Query: 57  SSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTAN-----SSFSVSY----PS-RSSNSSIT 116
           + P+ TN F+ NF L N     + HPY +  A      SSF ++     PS R++     
Sbjct: 223 TGPISTNKFYANFFLGNQTSTTFTHPYTMIWAKGDKNASSFGMAISHVEPSQRATGEPNN 282

Query: 117 QLPFFP-----------DLTITALNRTSPVSNRTHFVSSFSDLGVDLDIG----DFRFHL 176
           +LP  P            L ++A       +       +FS   +    G       F L
Sbjct: 283 KLPGNPVRYYINPVGIKSLVLSASELKESTTMSVAKPQAFSAQAILRPTGGSSESITFPL 342

Query: 177 VRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDST----KYFIRLSNGRSWVLY---SSS 236
           V+G  ++T ++      +I +    R V+          KY I L + ++W+LY    + 
Sbjct: 343 VQGMGFIT-AIYNNLQPAIQSAVLFRKVEPAGSPQGGIFKYKITLEDDKNWLLYVIPENG 402

Query: 237 AIYLVKSKKNQIVTS-GGFSGVIRVAVLPDSGIESEKILDQYRGCY----PVSGYAKLSG 296
           A   +K + N++++   GF GVI+VA  P S  E E I D+  G Y     +SG     G
Sbjct: 403 ADPKLKLEGNKLISGPTGFKGVIQVAKNP-SAEEGEGIYDKSAGSYATDIKISGSVGTDG 462

Query: 297 NFGFKYKWQKRGSGG-LLMLAHTLHRRLLPE-NQTVLQNLRYG-SIDGDLLGVVGDSWDL 356
              +K+ ++K G G  L+M A   H     +  +   +N++   +  G     VGDSW +
Sbjct: 463 TGTYKFSFEKAGKGAPLVMYALPHHVESFDDATKNTKKNMKLSTTTKGMATACVGDSWTM 522

Query: 357 NFDPIPIT-----WHSINGIDKKFFPEIVAALKRDA--ETLNVTELSSTAAS-YFYGKLL 416
               +P++     W   +            A+K  A  E     EL +   S YF GK L
Sbjct: 523 VEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNELSQDMELQTNLNSMYFSGKGL 582

Query: 417 ARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTKN--G 476
            + +      +E+          +  LK   + ++D K  +   +Y+  W G+V+     
Sbjct: 583 NKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNVWKGVVSSGTYE 642

Query: 477 ATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNFRPKKS 536
              T  DFG  +YNDHHFH GYF+ + A+L KLDP W +  KA    LV D  N      
Sbjct: 643 KGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNMLVRDSGNSVDNDE 702

Query: 537 QFSIPF-RNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGLSAAG 596
            F  PF R FD++  HSWA GL +  DG++QESTSE     YA  + G   GD+ + A G
Sbjct: 703 HF--PFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEARG 762

Query: 597 AVLTAAEIAATQTWWHVKEENSFYDEGFAEENRMVGILWSAARESRLWFAPPEWKECRVG 656
            ++          ++ +K +N    + F   N++ GIL+    +   +F      E   G
Sbjct: 763 NLMLGILARTLNNYFLMKNDNVNQPKNFI-GNKVTGILFENKIDHTTYFGAN--LEYIQG 822

Query: 657 IQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGE---GWKGFAYALEAVYDKKSA 662
           I + P++P +    S   F KE  E +  +   A A +   GWKG  YA  A+ D +++
Sbjct: 823 IHMLPLLPNSAYTRS-AEFVKEEWEAMFASGAAAPAEKVTGGWKGVLYANLAIIDPEAS 872

BLAST of Spg004486 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.3e-39
Identity = 164/672 (24.40%), Postives = 292/672 (43.45%), Query Frame = 0

Query: 45   PDPSKFFSPTLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSS 104
            P+P           P+ TN F+ N ++ + + P +++PY +    SS   SY     +++
Sbjct: 419  PNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSS---SYGFAVQHTT 478

Query: 105  ITQLPF-------FPDLTITALNRTSPVSNRTHFVSSFSDLGVDLDIGDFR--------- 164
            + Q  +         +  +  L     V + ++F SS +    ++ +   R         
Sbjct: 479  VDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRVVLSESNDS 538

Query: 165  -----FHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDST------KYFIRLSNGRS 224
                   LV+G  + T     + +  I +  G  ++ S   S       KY I L NG +
Sbjct: 539  SNYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYRITLLNGVT 598

Query: 225  WVLY-------SSSAIYLVKSKKNQIVTSGGFSG-VIRVAVLPDSGIESEKILDQYRGCY 284
            W+ Y       +S+   L  S + +I  S    G +I++AV P S  + E   DQ  G Y
Sbjct: 599  WLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAP-SETDYEVFYDQAAGMY 658

Query: 285  PVSGYAKLSG-------NFGFKYKWQKRGSGGLLMLAHTLHRR-----LLPENQTVLQ-- 344
              +   KL G        + F Y  Q   + G  M+    H       ++ +  T +Q  
Sbjct: 659  VTN--FKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQDYYTGIQLA 718

Query: 345  NLRYGSIDGDLLGVVGDSWDLNFDPIPITWHSINGID----KKFFPEIVAALKRDAETLN 404
            +   G ++G L   +  S  LN     + W S  G +     K   +++A +      ++
Sbjct: 719  STTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEVANSELQVS 778

Query: 405  VTELSSTAASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNG 464
            ++E  S   +Y+ GK++ + S + L   E+        + ++ +K+     L  +     
Sbjct: 779  ISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFDILLQNE-QTYP 838

Query: 465  FLYERKWAGLVTKN--GATSTTEDFGFGIYNDHHFHLGYFVYSIAVL----TKLDPNWGN 524
             +Y+ K+ GLV+    G+TST  DFG   YNDHHFH GY +++ AV+    +KL+  W  
Sbjct: 839  LIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVDSKLNGTWAA 898

Query: 525  RYKAQAYALVNDYMNFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNA 584
              K    +LV D  N   +K ++    R FD++  HSWAAGL +  +G+N+ES+SE  N 
Sbjct: 899  DNKDWVNSLVRDVAN-PSEKDEYFAQSRMFDWFNGHSWAAGLYENGNGKNEESSSEDYNF 958

Query: 585  YYAAALMGMAYGDEGLSAAGAVLTAAEIAATQTWWHVKEENSFYDEGFAEENRMVGILWS 644
             YA  L G   GD+ +   G ++ +    A   +++ + +N+   E     N++ GIL+ 
Sbjct: 959  AYAMKLWGATIGDQSMELRGDLMISIMKDAMNDYFYYQNDNTVEPEEII-GNKVSGILFD 1018

Query: 645  AARESRLWFAPPEWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGW 658
               +   +F      E   GI + P+ PV+  + S+    +E    + P +E  ++  GW
Sbjct: 1019 NIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQTKIEPIIESIES--GW 1077

BLAST of Spg004486 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 9.7e-39
Identity = 179/715 (25.03%), Postives = 297/715 (41.54%), Query Frame = 0

Query: 37  PEIASTAVPDPSKFFSPTLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSY 96
           P   S A P P    +  L   P+ TN F+ N  +     P + HPY +   N   S  Y
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNG--SSYY 73

Query: 97  PSRSSNSSITQLPFFPD-----------------LTITALNRTS----PVSNRTHFVSSF 156
               S+   +Q  F PD                 + I+A    S     +    HF    
Sbjct: 74  GLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQA 133

Query: 157 SDLGVDLDIGDFRFHLVRG-----SPYLTFSVLKTSSVSISTGNGVRSVDSYDDSTKYFI 216
           +        G     +V G       Y   + +  SS+  S+   +     Y    KY I
Sbjct: 134 TLSATTSGSGTIILPIVAGMGFVSGYYTNLTPVFNSSILFSSITKINFSRGY----KYRI 193

Query: 217 RLSNGRSWVLY-----SSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIES---EKIL 276
           +L++G+ W LY     SSS   L  +  +Q+ TS  F+G+I++  +P+  + +   + I 
Sbjct: 194 QLTDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIY 253

Query: 277 DQYRGCY--PVSGYAKLSGNFG-FKYKWQKRGSGGL--LMLAHTLHRRLL-PENQTVLQN 336
           D   G Y   +S  A++SG  G + +++   G   L  LM A   H +    + Q     
Sbjct: 254 DASAGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSFGSDTQAYKTG 313

Query: 337 LRYGSID-GDLLGVVGDSWDL-------NFDPIPITWHSINGIDKKFFPEIVAALKRDAE 396
           L   S   G +      +W L           +PI W   NG    + P  +AA++    
Sbjct: 314 LGLASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPW---NGGSNTYSPTALAAIRAACA 373

Query: 397 T---LNVTELSSTAASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDG 456
           T    +V   S+  + Y  GK++A  +++ L+A  +   + +    +  LK  +      
Sbjct: 374 TDINFDVVNASNLDSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAM-----A 433

Query: 457 KFAKN----GFLYERKWAGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDP 516
           +F  N      +Y+  + G+++  G +S   D+G   YNDHHFH GY +Y+ AV+  LDP
Sbjct: 434 RFTTNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDP 493

Query: 517 NW-GNRYKAQAYALVNDYMNFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTS 576
           +W  N       AL+ D  N     + F++ FRNFD++  HSWA G+ +  DG+++ESTS
Sbjct: 494 SWLVNDNIRYVNALLRDSANPSESDTYFAM-FRNFDWFVGHSWATGIFESGDGKDEESTS 553

Query: 577 EAVNAYYAAALMGMAYGDEGLSAAGAVLTAAEIAATQTWWHVKEENSFYDEGFAEENRMV 636
           E  N  YA  L GM   D  L     ++ A    +  T+ ++    S         N + 
Sbjct: 554 EDFNFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNTYIYMTPTTSVQPSQIL-GNYVT 613

Query: 637 GILWSAARESRLWFAPPEWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERAD 692
           GI +    +   +F+  E+  C+ GI + P  P++  + S     ++    ++P +    
Sbjct: 614 GITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSPSYVQQDWNAKIAPIIN--T 673

BLAST of Spg004486 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 7.2e-34
Identity = 176/709 (24.82%), Postives = 301/709 (42.45%), Query Frame = 0

Query: 11  IFFFPLLTALMALSDHPPPST---TQFPFPEIASTAVPDPSKFFSPTLLSSPLPTNSFFQ 70
           IF  P+L+ L A     PPS     Q P P+     + D S          PL TN F+ 
Sbjct: 75  IFQSPVLSNLKA-----PPSVFNKVQHPVPK---PNIDDQS--------VDPLETNKFYT 134

Query: 71  NFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSS-----ITQLPFFPDLTITALNRT 130
           N +L++   P + HPY I  +         +  + +S      T  P  P       N  
Sbjct: 135 NMLLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLASQRVFDTTTNP--PRFYFNPTNIK 194

Query: 131 SPVSNRTHFVSS------FSD-------LGVDLDIGDF-RFHLVRGSPYLTFSVLKTSSV 190
           S V     FVSS      F D       L + L    F  F LV+G  ++T ++      
Sbjct: 195 SFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVT-AIYHDLGF 254

Query: 191 SISTGNGVRSVDSYDDST---KYFIRLSNGRSWVLYSSSAIY-------LVKSKKNQIVT 250
            + +  G RS++    +    KY I+L N R+W+LY +S  Y       +     N I++
Sbjct: 255 ELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNTIIS 314

Query: 251 SGGFSGVIRVAVLPDSGIESEKILDQYRGCYPVSGYAKLSG--------NFGFKYKWQKR 310
           S   +G+I          +S   +D   GCYPV  Y  LSG        N+ F Y     
Sbjct: 315 SHKINGLI-----CQLSADSVPSIDMAAGCYPV--YCDLSGQTVDEHFTNYRFNYTVAGY 374

Query: 311 G-SGGLLMLAHTLHR-RLLPENQ-TVLQNLRYGSIDGDLLGVVGDSWD--------LNFD 370
             SG  LM A   H+    PE Q   + +    ++ G + G + +S+D        L F+
Sbjct: 375 SQSGTTLMYALPHHKAAFTPEMQEREIASSLDSTVKGLMTGYLTNSFDMQVQVPQELGFE 434

Query: 371 PIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAASYFYGKLLARASRLALIAE 430
           P+ ++ +      ++   +I  A  ++ +  +  + S+  + YF GK+LA+ + +  +  
Sbjct: 435 PVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILAKYAWILYVTH 494

Query: 431 EVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTKNGATSTTEDFGFGIY 490
            + +   +   ++  L   ++ ++  +       Y+  W G+++     S+++DFG   Y
Sbjct: 495 YILHDENLTKELLSKLTIAMERFISNQQVL-PLNYDVSWKGIISSG---SSSQDFGNSYY 554

Query: 491 NDHHFHLGYFVYSIAVLTKLDP--------NWGNRYKAQAYALVNDYMNFRPKKSQFSIP 550
           NDHHFH  Y V + A+++ +D         +W    +     L+ DY         F   
Sbjct: 555 NDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVDNDDPYFP-Q 614

Query: 551 FRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGLSAAGAVLTAA 610
           FR+FD++  HSWA GL    DG+++ESTSE VN+ YA  L G+  G+  L+    +    
Sbjct: 615 FRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANLQLGI 674

Query: 611 EIAATQTWWHVKEENSFYDEGFAEENRMVGILWSAARESRLWFA-PPEWKECRVGIQVFP 660
                Q+++  +  N+   + F   N++ GIL+    +   +F   P++      I   P
Sbjct: 675 MRNVFQSYFLYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEPQYIHM---IHAIP 734

BLAST of Spg004486 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.2e-28
Identity = 119/494 (24.09%), Postives = 215/494 (43.52%), Query Frame = 0

Query: 187 KYFIRLSNGRSWVLY-SSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKILDQY 246
           KY + +S+   W++Y    ++ L +S    +V S  F+G I++A +P     +E + D Y
Sbjct: 226 KYRVTMSDNHVWLIYIYGDSLTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTY 285

Query: 247 RGCY----PVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTL-HRRLLPENQTVLQNLRYG 306
            G Y     +SGY + +  + + + +   G   +  L   L H+     + T + ++   
Sbjct: 286 AGVYITGISISGYVEDAVGY-YSFDFTTAGDTSVEPLFFLLPHQVDTAVSGTKVTSIVLA 345

Query: 307 S-IDGDLLGVVGDS--------WDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLN 366
           S + GD+    G+S         D+ F P   T   I   ++    EI+A +       +
Sbjct: 346 SLVSGDMNAAAGNSITFAEAIPQDIGFLPWSPTGGQIGYSEEAL--EIIAEVAGTELGED 405

Query: 367 VTELSSTAASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNG 426
            +  S+  + Y+ GK+LA+ + L +   ++          ++ L+     ++D +     
Sbjct: 406 FSANSNLNSMYYSGKVLAKYAMLCVTINDILGDETSSEQCIQKLEAAFARFVDNQ-QIYP 465

Query: 427 FLYERKWAGLVTKNGAT-STTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGN--RYK 486
             Y+  W G+V+  G +  +  DFG   YNDHHFH GYFV++ AV+  +DP+W N    K
Sbjct: 466 LTYDNTWKGVVSVAGLSGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNK 525

Query: 487 AQAYALVNDYMNFRPKKSQFSIP-FRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYY 546
                LV D  N  P  +    P  R  D +  H WA+GL +  DG+++ESTSE  N ++
Sbjct: 526 EWVNFLVRDVAN--PSSNDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFF 585

Query: 547 AAALMGMAYGDEGLSAAGAVLTAAEIAATQTWWHVKEENSFYDEGFAEENRMVGILWSAA 606
              L G   GD  +     ++   E  A   +    + N        + N + GI +   
Sbjct: 586 GMKLWGQVIGDSDMEDRANIILGIERNALNKYMLYADGN--VQPTSMQPNYVAGITFMNK 645

Query: 607 RESRLWFAPPEWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKG 662
                +F      E   GI + P+ P++  +        E    ++  ++  D+  GW+ 
Sbjct: 646 ITHTTYFGTN--IEYIQGIHMLPITPISAFIRGPSFVLAEWNALLASVIDYVDS--GWRS 705

BLAST of Spg004486 vs. ExPASy TrEMBL
Match: A0A6J1EFF1 (Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111432794 PE=3 SV=1)

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 575/694 (82.85%), Postives = 618/694 (89.05%), Query Frame = 0

Query: 1   METPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPL 60
           ME P PWL+  F   LL A  A SD  PPS+ QFPFPE  STAVPDP+KFFSP+LLSSPL
Sbjct: 1   MEAPNPWLLPFFLLLLLAA--AFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPL 60

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALN 120
           PTNSFFQNFVLNNGDLPEYIHPYLIRTANSS SVSYPSR+SNSSITQLPF PDLTI    
Sbjct: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTI---- 120

Query: 121 RTSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GNGVRSV 180
             S VSN+THFVSSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSS+SIST GNGV SV
Sbjct: 121 --SSVSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSISISTGGNGVGSV 180

Query: 181 DSYDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESE 240
           DSYD STK+ IRLSNGRSWV+YS++AIYL+K K ++IVTSGGFSGVIRVAVLP+S  E+E
Sbjct: 181 DSYDGSTKHIIRLSNGRSWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAAETE 240

Query: 241 KILDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRY 300
           +ILD+Y GCYPVSGYAKLSGNFGF YKWQK+GSGGLLMLAH LHRR+L EN TVLQN +Y
Sbjct: 241 QILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQNFKY 300

Query: 301 GSIDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTA 360
           GSIDGDL+GVVGDSWDLNF PIPITWHSINGI++KFFPEIVAALK D  TLNVTELSSTA
Sbjct: 301 GSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTA 360

Query: 361 ASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWA 420
           ASYFYGKLLARA+RLALIAEEVDYAAGVIPAVVKFLK G+QPWLDGKF +NGFLY+RKWA
Sbjct: 361 ASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDRKWA 420

Query: 421 GLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYM 480
           GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVL KLD NWGN+YKA AYALV DYM
Sbjct: 421 GLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVYDYM 480

Query: 481 NFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDE 540
           N+R K SQFSIPFRNFDFWK HSWAAGLT+FPDGRNQESTSEA+NAYYAAALMG+AYGDE
Sbjct: 481 NYRSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDE 540

Query: 541 GLSAAGAVLTAAEIAATQTWWHVKE--ENSFYDEGFAEENRMVGILWSAARESRLWFAPP 600
            L+A G+ LTAAEIAA QTWWHV     +S YDEGF EENR+VGILWS ARESRLWFA  
Sbjct: 541 SLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFATA 600

Query: 601 EWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYD 660
           EW+ECRVGIQV PVVPVTERVF+D GF KEVVEWVSPALER DAGEGWKGF YALE +YD
Sbjct: 601 EWRECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYD 660

Query: 661 -KKSAVGKLKKLKKHDDGNSLSNLLWWIYSRPGR 691
            K++AVGK+KKLKKHDDGNSLSNLLWWIYSRP R
Sbjct: 661 NKENAVGKVKKLKKHDDGNSLSNLLWWIYSRPER 686

BLAST of Spg004486 vs. ExPASy TrEMBL
Match: A0A5A7TDJ6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001320 PE=3 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 574/683 (84.04%), Postives = 611/683 (89.46%), Query Frame = 0

Query: 11  IFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPLPTNSFFQNFV 70
           +FFF LLTA         P + QFPFPE  STAVPDPSKFFSP LLSSPLPTNSFFQNFV
Sbjct: 13  LFFFLLLTA--------APVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFV 72

Query: 71  LNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALNRTSPVSNRTH 130
           LNNGD PEYIHPYLIRTANSS  VSYPSR SNSSI QL FFPDL I++LN+T   SN TH
Sbjct: 73  LNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATH 132

Query: 131 FVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDSTKYFI 190
           FVSSFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V IST NGVRSVDSY+D TKY I
Sbjct: 133 FVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYII 192

Query: 191 RLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKILDQYRGCYP 250
           RL+NGRSW+LYSSSAIYL+KSK NQ+VTSGGF GVIRVAVLPDS +ESEKILD+Y GCYP
Sbjct: 193 RLNNGRSWLLYSSSAIYLIKSKSNQVVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYP 252

Query: 251 VSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRYGSIDGDLLGVV 310
           VSG+AKLSG FGF+YKWQK+GSGGLLMLAHTLHR +LP +QTVLQNLRY SIDGDLLGVV
Sbjct: 253 VSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVV 312

Query: 311 GDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAASYFYGKLLAR 370
           GDSWDL F+PIPITWHSINGID KFFPEIVAALKRD  TLNVTELSST ASYFYGKLLAR
Sbjct: 313 GDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLAR 372

Query: 371 ASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTKNGATST 430
           A+RLALIAEEV+Y A VIPAVVKFLKNGIQPWL GKF KNGFLYERKWAGLVTKNGATST
Sbjct: 373 AARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATST 432

Query: 431 TEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNFRPKKSQFSI 490
            EDFGFGIYNDHHFHLGYFVYSIAVL KLDPNWG +YK QAYAL+ DYMNFRPKKSQFSI
Sbjct: 433 IEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSI 492

Query: 491 PFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGLSAAGAVLTA 550
           PFRNFDFWK HSWAAGL +FPDGRNQESTSEAVNAYYAAALMG+AYGD  L+AAG+ LTA
Sbjct: 493 PFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTA 552

Query: 551 AEIAATQTWWHV-KEENSFYDEGFAEENRMVGILWSAARESRLWFAPPEWKECRVGIQVF 610
           AEI A+QTWWHV KE+N  YD+GFAEENRMVGILWSAARESRLWFAP EW+ECR+GIQV 
Sbjct: 553 AEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVL 612

Query: 611 PVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKKSAVGKLKKLK 670
           PV+PVTER+F+DVGF KE VEWV PALER DAGEGWKGFAYALE +YDKKSAV K+KKLK
Sbjct: 613 PVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLK 672

Query: 671 KHDDGNSLSNLLWWIYSRP-GRG 692
           KHDDGNSLSNLLWWIYSRP GRG
Sbjct: 673 KHDDGNSLSNLLWWIYSRPEGRG 684

BLAST of Spg004486 vs. ExPASy TrEMBL
Match: A0A6J1I8S4 (Endo-1,3(4)-beta-glucanase OS=Cucurbita maxima OX=3661 GN=LOC111472032 PE=3 SV=1)

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 572/693 (82.54%), Postives = 619/693 (89.32%), Query Frame = 0

Query: 1   METPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPL 60
           M    PWL+   FF LL A  A SD  PPS+ QFPFPE  STAVPDP+KFFSP+LLSSPL
Sbjct: 1   MAATNPWLLP--FFLLLAA--AYSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLSSPL 60

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALN 120
           PTNSFFQNFVLN GDLPEYIHPYLIRTANSS SVSYPSR+SN+SITQLPF PDLTI    
Sbjct: 61  PTNSFFQNFVLNKGDLPEYIHPYLIRTANSSLSVSYPSRTSNTSITQLPFSPDLTI---- 120

Query: 121 RTSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSIST-GNGVRSV 180
             S VSN+THFVSSFSDL VDLDIG+FRFHLVRGSPYLTFSVLKTSSVSIST GNGV SV
Sbjct: 121 --SSVSNKTHFVSSFSDLAVDLDIGEFRFHLVRGSPYLTFSVLKTSSVSISTGGNGVDSV 180

Query: 181 DSYDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESE 240
           DSYD STK+ IRLSNGR+WV+YS++AIYL+K+K ++IVTSGGFSGVIRVAVLP+S  E+E
Sbjct: 181 DSYDGSTKHIIRLSNGRNWVVYSTAAIYLMKAKSSEIVTSGGFSGVIRVAVLPNSAAETE 240

Query: 241 KILDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRY 300
           +ILD+Y GCYPVSGYAKLSGNFGF YKWQK+GSGGLLMLAH LHRR+LPEN TVLQN +Y
Sbjct: 241 QILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLPENLTVLQNFKY 300

Query: 301 GSIDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTA 360
           GSIDGDL+GVVGDSWDLNF PIPITWHSINGI++KFFPEIVAALK D  TLNVTELSSTA
Sbjct: 301 GSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELSSTA 360

Query: 361 ASYFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWA 420
           ASYFYGKLLARA+RLALIAEEVDYAAGVIPAVVKFLK GIQPWLDGKF +NGFLY+RKWA
Sbjct: 361 ASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGIQPWLDGKFGRNGFLYDRKWA 420

Query: 421 GLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYM 480
           GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVL KLD NWGN+YKA AYALV+DYM
Sbjct: 421 GLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVHDYM 480

Query: 481 NFRPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDE 540
           N+R + +QFSIPFRNFDFWK HSWAAGLT+FPDGRNQESTSEA+NAYYAAALMG+AYGDE
Sbjct: 481 NYRSRNTQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAYGDE 540

Query: 541 GLSAAGAVLTAAEIAATQTWWHVKE--ENSFYDEGFAEENRMVGILWSAARESRLWFAPP 600
            L+A G+ LTAAEIAA QTWWHV     +S YDEGF EENR+VGILWS ARESRLWFA  
Sbjct: 541 SLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWFASA 600

Query: 601 EWKECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYD 660
           EW+ECRVGIQV PVVPVTERVF+D GF KEVVEWVSPALER DAGEGWKGF YALE +YD
Sbjct: 601 EWRECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEGIYD 660

Query: 661 KKSAVGKLKKLKKHDDGNSLSNLLWWIYSRPGR 691
           KK+AVGK+KKLKKHDDGNSLSNLLWWIYSRP R
Sbjct: 661 KKNAVGKVKKLKKHDDGNSLSNLLWWIYSRPER 683

BLAST of Spg004486 vs. ExPASy TrEMBL
Match: A0A0A0KW06 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G647400 PE=3 SV=1)

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 575/688 (83.58%), Postives = 608/688 (88.37%), Query Frame = 0

Query: 2   ETPKPWLICIFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPLP 61
           ET KP  I   FF LL    A+S        QFPFPE  STAVPDP KFFSP LLSSPLP
Sbjct: 3   ETSKPSPISTLFFLLLLTAAAVS-------AQFPFPETTSTAVPDPGKFFSPNLLSSPLP 62

Query: 62  TNSFFQNFVLNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALNR 121
           TNSFFQNFVLNNGDLPEYIHPYLIRTANSS SVSYPSR SNSSITQL FFPDL I++  +
Sbjct: 63  TNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKK 122

Query: 122 TSPVSNRTHFVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDS 181
           T    N THF+SSFSDLGVDLDIG FRFHLVRGSPYLTFSVLKTSSV IST NGVRSVDS
Sbjct: 123 T---FNTTHFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDS 182

Query: 182 YDDSTKYFIRLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKI 241
           Y+D TK+ IRL+NGRSWVLYSSSAIYLVKSK NQIVTSGGF GVIRVAVLPDS +ESEKI
Sbjct: 183 YEDYTKHIIRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKI 242

Query: 242 LDQYRGCYPVSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRYGS 301
           LD+Y GCYPVSG+ KL G FGF+YKWQK+GSGGLLMLAHTLHR +LP +QTVLQN+RY S
Sbjct: 243 LDRYSGCYPVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSS 302

Query: 302 IDGDLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAAS 361
           IDGDLLGVVGDSWDL F+PIPITWHSINGID KFFPEIVAALKRD  TLN TELSS  AS
Sbjct: 303 IDGDLLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPAS 362

Query: 362 YFYGKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGL 421
           YFYGKLLARA+RLALIAEEV+  AGVIPAVVKFLKNGIQPWL GKF KNGFLYERKWAGL
Sbjct: 363 YFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGL 422

Query: 422 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNF 481
           VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVL KLDPNWG +YK QAYAL+ DYMNF
Sbjct: 423 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNF 482

Query: 482 RPKKSQFSIPFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGL 541
           RPKKSQFSIPFRNFDFWK HSWAAGLT+FPDGRNQEST+EAVNAYYAAALMG+AY DE L
Sbjct: 483 RPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESL 542

Query: 542 SAAGAVLTAAEIAATQTWWHVKEENS-FYDEGFAEENRMVGILWSAARESRLWFAPPEWK 601
           +AAG+ LTAAEI ATQTWWHVK EN+  YD+GF EENRMVGILWSAARESRLWFAP EW+
Sbjct: 543 TAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWR 602

Query: 602 ECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKKS 661
           ECRVGIQV PV+PVTERVF+DVGF KEVVEWV PALER DAGEGWKGFAYALE +YDKKS
Sbjct: 603 ECRVGIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKS 662

Query: 662 AVGKLKKLKKHDDGNSLSNLLWWIYSRP 689
           AV K+KKLKKHDDGNSLSNLLWWIYSRP
Sbjct: 663 AVEKVKKLKKHDDGNSLSNLLWWIYSRP 680

BLAST of Spg004486 vs. ExPASy TrEMBL
Match: A0A142BTQ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=glu2 PE=2 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 574/683 (84.04%), Postives = 610/683 (89.31%), Query Frame = 0

Query: 11  IFFFPLLTALMALSDHPPPSTTQFPFPEIASTAVPDPSKFFSPTLLSSPLPTNSFFQNFV 70
           +FFF LLTA         P + QFPFPE  STAVPDPSKFFSP LLSSPLPTNSFFQNFV
Sbjct: 13  LFFFLLLTA--------APVSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFV 72

Query: 71  LNNGDLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALNRTSPVSNRTH 130
           LNNGD PEYIHPYLIRTANSS  VSYPSR SNSSI QL FFPDL I++LN+T   SN TH
Sbjct: 73  LNNGDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATH 132

Query: 131 FVSSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDSTKYFI 190
           FVSSFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V IST NGVRSVDSY+D TKY I
Sbjct: 133 FVSSFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYII 192

Query: 191 RLSNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKILDQYRGCYP 250
           RL+NGRSWVLYSSSAIYL+KSK NQIVTSGGF GVIRVAVLPDS +ESEKILD+Y GCYP
Sbjct: 193 RLNNGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYP 252

Query: 251 VSGYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQTVLQNLRYGSIDGDLLGVV 310
           VSG+AKLSG FGF+YKWQK+GSGGLLMLAHTLHR +LP +QTVLQNLRY SIDGDLLGVV
Sbjct: 253 VSGFAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVV 312

Query: 311 GDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAASYFYGKLLAR 370
           GDSWDL F+PIPITWHSINGI+ KFFPEIVAALKRD  TLNVTELSST ASYFYGKLLAR
Sbjct: 313 GDSWDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLAR 372

Query: 371 ASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTKNGATST 430
           A+RLALIAEEV+Y A VIPAVVKFLKNGIQPWL GKF KNGFLYERKWAGLVTKNGATST
Sbjct: 373 AARLALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATST 432

Query: 431 TEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNFRPKKSQFSI 490
            EDFGFGIYNDHHFHLGYFVYSIAVL KLDPNW  +YK QAYAL+ DYMNFRPKKSQFSI
Sbjct: 433 IEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSI 492

Query: 491 PFRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGLSAAGAVLTA 550
           PFRNFDFWK HSWAAGL +FPDGRNQESTSEAVNAYYAAALMG+AYGD  L+AAG+ LTA
Sbjct: 493 PFRNFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTA 552

Query: 551 AEIAATQTWWHV-KEENSFYDEGFAEENRMVGILWSAARESRLWFAPPEWKECRVGIQVF 610
           AEI A+QTWWHV KE+N  YD+GFAEENRMVGILWSAARESRLWFAP EW+ECR+GIQV 
Sbjct: 553 AEITASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVL 612

Query: 611 PVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKKSAVGKLKKLK 670
           PV+PVTER+F+DVGF KE VEWV PALER DAGEGWKGFAYALE +YDKKSAV K+KKLK
Sbjct: 613 PVLPVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLK 672

Query: 671 KHDDGNSLSNLLWWIYSRP-GRG 692
           KHDDGNSLSNLLWWIYSRP GRG
Sbjct: 673 KHDDGNSLSNLLWWIYSRPEGRG 684

BLAST of Spg004486 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 741.1 bits (1912), Expect = 8.0e-214
Identity = 377/681 (55.36%), Postives = 475/681 (69.75%), Query Frame = 0

Query: 24  SDHPPPS---------TTQFPFPEIASTAVPDPSKFFSPTLLSSPLPTNSFFQNFVLNNG 83
           S  PPPS          T F FP   S+ +PDPS+FFS  LLSSPLPTNSFFQNF LNNG
Sbjct: 36  SPSPPPSQQMSSSRQKNTPFLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNG 95

Query: 84  DLPEYIHPYLIRTANSSFSVSYPSRSSNSSITQLPFFPDLTITALNRTSPVSNRTHFVSS 143
           D  EY HPY+I+ + SS S+SYPS S NS+     F  D+TIT  +   P S ++H +SS
Sbjct: 96  DQAEYFHPYIIKPSTSSLSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISS 155

Query: 144 FSDLGVDLDI--GDFRFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDSTKYFIRL 203
           FSDLGV LD    + RF LVRGSP++TFSV   SS++IST + V S+     STKY ++L
Sbjct: 156 FSDLGVTLDFPSSNLRFFLVRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKL 215

Query: 204 SNGRSWVLYSSSAIYLVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKILDQYRGCYPVS 263
           +N ++W++Y+SS I L K   + I    GFSG+IR+ VLP+     E ILD +   YPVS
Sbjct: 216 NNNQTWLIYASSPINLTKDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVS 275

Query: 264 GYAKLSGNFGFKYKWQKRGSGGLLMLAHTLHRRLLPENQ---TVLQNLRYGSIDGDLLGV 323
           G A  +  F  +YKW+KRG G LLMLAH LH +LL  N    TVL N +Y SIDGDL+GV
Sbjct: 276 GDADFTKPFALEYKWEKRGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGV 335

Query: 324 VGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAASYFYGKLLA 383
           +GDSW L  DP+ +TWHSI G+ +    EI++AL +D   L+ +    T +SYFY KL+A
Sbjct: 336 IGDSWVLKPDPVSVTWHSIKGVQEDSHQEIISALIKDVNALD-SSAEVTNSSYFYAKLIA 395

Query: 384 RASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTKNGATS 443
           RA+RLALIAEEV Y   VIP +  +LKN I+PWL+G F  NGFLY+ KW G++TK G+  
Sbjct: 396 RAARLALIAEEVCY-LDVIPKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRD 455

Query: 444 TTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNFRPK--KSQ 503
           +  DFGFGIYNDHH+HLGYFVY+IAVL K+DP WG RY+ QAY L+ DY+    K  KS 
Sbjct: 456 SGADFGFGIYNDHHYHLGYFVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSN 515

Query: 504 FSIP-FRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEGLSAAGA 563
            + P  R FD +K HSWA GLT+F DGRNQESTSEAVNAYY+AAL+G+AYGD  L AA +
Sbjct: 516 SNYPRLRCFDLFKLHSWAGGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAAS 575

Query: 564 VLTAAEIAATQTWWHVKEENSFYDEGFAEENRMVGILWSAARESRLWFAPPEWKECRVGI 623
           ++   EI A + WW VKE+++ Y + F  ENR+VG+LWS  R+S LWFAP EWKECR+GI
Sbjct: 576 MVLTLEIHAAKMWWQVKEDDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGI 635

Query: 624 QVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKKSAVGKLK 683
           Q+ P++PV+E +FSDV F K++V W  PAL R   GEGWKGF YALE++YDK  A+ K+K
Sbjct: 636 QLLPLLPVSEVLFSDVTFVKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIK 695

Query: 684 KLKKHDDGNSLSNLLWWIYSR 688
            L   DDGNSLSNLLWW++SR
Sbjct: 696 GLNGFDDGNSLSNLLWWVHSR 712

BLAST of Spg004486 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 680.2 bits (1754), Expect = 1.7e-195
Identity = 351/687 (51.09%), Postives = 442/687 (64.34%), Query Frame = 0

Query: 30  STTQFPFPEIASTAVPDPSKFFSPTLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLIRTAN 89
           S   F FPE  S+ +PDPS+FFSP LLS+PLPTNSFFQNF L NGD  EY HPYLI++ +
Sbjct: 4   SNALFLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVD 63

Query: 90  SSFSVSYPSRSSNSSITQLPFFPDLTITALNRTSPVSNRTHFVSSFSDLGVDLDI--GDF 149
           SS  +SYPS   NS      F  D+ I+  N   P S +TH +SSFSDL V LD    + 
Sbjct: 64  SSLCISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNL 123

Query: 150 RFHLVRGSPYLTFSVLKTSSVSISTGNGVRSVDSYDDSTKYFIRLSNGRSWVLYSSSAIY 209
           RF LVRGSP                            STK+  +L+N + W++Y+SS I 
Sbjct: 124 RFFLVRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPID 183

Query: 210 LVKSKKNQIVTSGGFSGVIRVAVLPDSGIESEKILDQYRGCYPVSGYAKLSGNFGFKYKW 269
           L K   + I   GGF+G++R+ VLP S    E  LD++  CYPVSG A  +  F  KY W
Sbjct: 184 LTKHGDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMW 243

Query: 270 QKRGSGGLLMLAHTLHRRLLPENQ-----------------------TVLQNLRYGSIDG 329
           +KRGSG LLMLAH LH +LL ++                        TVL + RY SIDG
Sbjct: 244 EKRGSGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDG 303

Query: 330 DLLGVVGDSWDLNFDPIPITWHSINGIDKKFFPEIVAALKRDAETLNVTELSSTAASYFY 389
           DL+GVVGDSW L  D + +TWHS+ G+ K  + EI++AL +D   LN +    T++SYFY
Sbjct: 304 DLVGVVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLN-SSAEVTSSSYFY 363

Query: 390 GKLLARASRLALIAEEVDYAAGVIPAVVKFLKNGIQPWLDGKFAKNGFLYERKWAGLVTK 449
           GKL+ARA+R ALIAEEV Y   VIP +V +LKN I+PWLDG F  NGFLY+ KW GL+TK
Sbjct: 364 GKLIARAARFALIAEEVCY-LDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITK 423

Query: 450 NGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLTKLDPNWGNRYKAQAYALVNDYMNFRPK 509
            G+  +  DFGFGIYNDHH+H+GYF+Y+IAVL K DP WG RY+AQAY+L+ D+M F  K
Sbjct: 424 QGSKDSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRK 483

Query: 510 ---KSQFSIP-FRNFDFWKHHSWAAGLTQFPDGRNQESTSEAVNAYYAAALMGMAYGDEG 569
               S  S P  RNFD +K HSWA GLT+F DGRNQESTSEAVNAYY+AAL+G+AYGD+ 
Sbjct: 484 DDNNSNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKH 543

Query: 570 LSAAGAVLTAAEIAATQTWWHVKEENSFYDEGFAEENRMVGILWSAARESRLWFAPPEWK 629
           L    + +   EI A + WW VK+  + Y + F  ENR+VG+LWS  R+S LWF P EWK
Sbjct: 544 LVETASTIMTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWK 603

Query: 630 ECRVGIQVFPVVPVTERVFSDVGFAKEVVEWVSPALERADAGEGWKGFAYALEAVYDKKS 688
           ECR+GIQ+ P++P              +V W  PAL+R   GEGWKGF YALE++YDK  
Sbjct: 604 ECRLGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDG 648