Spg004428 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg004428
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DNA primase
Location	scaffold9: 9464498 .. 9471298 (-)
RNA-Seq Expression	Spg004428
Synteny	Spg004428

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAGACCCACGACCATCCGCTTCAGATCTGCGGCTTCACGGCGAACGGACGACAGCTTCATGGCCATCCGTTTCAGGCCCACGACGAGCAGCAGCTTCACAGCGAATGGACGACATAATGGACAAAGAACCTCGTTTTGGGTTCAGATCTGAAAGATGAGTCTGTTTTAGTTTTTTTTAAGAGAAGTTAGAGAAGAAGGAGAGAAGAGTCGAAGAAAAGAAGAGAAAATAAAAAAAAATAAAAAGAAGTCGCTGGCGAGCGGTGGTCGCCGGCAGCCGGCGGTGTGTGTGGTGAAAAAGAAGAAGAAGGTGGAAGGTGGAGAGAGAAAATGAATAAATAAATTAAAAAAATATATTTTTATTATTTTAATATTATTTTATTTTAAAAAATTTAAAAATAATTTTTTTAATAAAAAAATAGTGTCACGTCAGCAATCCGTTAATTATTAACGAAAAATTGGATAGAGGGACCAAAATGAAACTAAAATCAAATCTTATGGACTAAATTGTTAAGTTTGAAAACTCAAGGACCAAAATAAAAGCTAACTCAAACCTTAGAAACTAAAAGTGTATTTTTCCCATTAAAAAAACTAACAACCTTGTTTATTTATTTATCTTTCCTTTACTATATTTTTAATATATTATATATTTATATTAAAAAGAGAGAAAAGTAAACCCTACACTCCATCTTTCTTTCTTCCCCTTTAACTTCCCGCCAAAACTTCTATCTTGTCCCAATTTTTTAGTCGACTCCCGCTATTTCTGTTCCTAATTATCCTTTGTCGTTGCATAGTCTAATTCGAGTCCCGGTATTCCAATTTGTAAAAATAACAACATATCTTCTCTTAGTCCCCGTTCAGAGCAGGCGTGGGTCTCGATTTGAGTGGCTGATTTAGGCTGTTTTCAGTAAAAGCTTCAGACGGCGTTGTTGTTGTTTTTCGGCGTGGGTGATTTCTCTGTGGGTATTCTCTTGAAGCTCGAATCAATCGAGGTTTGAGGTATTTTACATTGGGTTAAATTTAACGCCATTGTTGTACTCTGTCTTGTTGACTTGACGCTCTTAATAAATCTCTTATAAGTTGGTTCTCAAAGTGGATGTAGACTGGAAGGTTGAACCACTATATATCCTTGTGTTTTTTTTTCTCTATTGCTTGATTATTTGCTGCTGGAATCTGTGTTCTGATTGGGCATATTAAGTTTGCTTTAAATTCTGCACAACTTTCCGATCGAACTTCCTAACCTGTCTCCACTGCACTTGCGTTTCGTAATTCTTAACATCTCGCTCTCCTTTTCTTTTTCTTTTACGATCTACGATTCATTTCATTCTCGGGTTGAAAAATTTCGTCTCTGCTTGAAATTTGCAATAAATGCAGGTACTGTAGCGAAAATGGTTAACGAAGTGGTTGAGAATGGAGTAAGCGATATGGTAATTGATGGTAACGAACAAAAGCCGAAGAAAATTGTGCCTGATGGGTTCGATGCTAATTATCTCAAAATTTACTACGGTGAACCATTTATACCTCTTCGATTTTTTAGAAATTTTTGTGTTGAATTGTCCCATGAAATTTAGTTTACTTGTCTGTTTTATGTTTTTCTGCTACTGAAAGATATGGAATAAAATTGTTGCAGGGAAGCTTTTTCCGTATACTGATTTTTTCAAATGGATGTCTTATGGAAATGGTAAGCCCTTGATTCTTGGAAACAAAAACAAAATATTACCCCCAGAGGAAGATAAATATTGTCATATATGTAACTTTTCCTGCTATATATATGTGCTTGAAGATGGAAAGCATCCAGGCAGTGACCGATCCTATTTTGGCCGAAGAGAGTTCTCATTCACGTTGGATAATGATATTTATCTACGATTCCAATCTTTCAACAGTTTGTCTGAAATGGAAAATTCGATCAAGGAAAAGTGTCCGTTTAAGATTGATATTGGGTCTGTATACAGCGTGGATGTAAGTAAAATTCTTCCTTGTTCTTATCATGAATCTGATATTGTGCTCTCCCCCATCAATATTGTTTGATTTTTTTGGGACTAGGTTTTTTTATTCAACTTTAGCATCACCTTCAATGGAAAGGTATTCCAAGGATGGATATGTAGAATTTGAGTATTTAAAAGACAATTTAAATTTTCTTGTTGAAAGAAAATATGGTAGTGGTTGTCAAAGTGAGCATAACTCAACGGTAATTGACATGTACTCTTGACCAAGAAGTCTGAGATTCAAATCCGAGATCCCAAATGTTGTTGAACTCAAAAGAAAAAAAAAGATGTGGTAGTGGTTGTTTTGAAGGAGTTCTTATAGTGATTCTGGGTACTTAACTGGCTTCTTGGTTTGTTCTTTGGTTTGCTTTTGGTTTGTTTAGTACGGGGGCTTTCCTTTTAGCCCACTGTCTTTGGTTGGTTGTTGGTACTTTCTCTCTCTCTTTCTGGAAGTTTTTTCCTTGAACATTTTTTTTAATCTTTTCTTCTATCAATAAAAAGTTGTTTCTTGTCTAAAATCTAAAAGAAATAAAAGTGAAAGAATGGAAGTGAATAATCATGAAACTTCTATGCTTTGAAATTTGAGGTTTGACTTGCCTCTGACAGAAATAAGTAGGGATGATGATTCTATGGGGAGTGAATTGCAGTGGATTGTCTAAGGTTCCTAGAATCAACTAAAGTCTCAAGCTCAGCAAGTGCATGAGAATGATTGTCATTTAGTTTAGAATAGATAAGTTGAGTTGGAGGACTTGTTATGTCATATGTGTAAAGTTTGAGATAGAAATGTAGTTCCAGTTGCTACAACCGAGTTAGTAGATGACAAGCATCATTTAGTTTGTAAGTCTCTTAGGGGAACAGGCATGATTAAGCAGTGAATGGATATGACTCTCTTATCAAGAAAGGAAACGGAAATGTAAGTGAAGGAAGAAAATGAAAATGTAACGGCATGGTTACTTGGGGAGATTCAAAGCCCGATGAATTCTTTAAAGCAACTAGTTTTTAACAGTTATTCATAAAAAATAAAAAATAAATAAAAAACTGGTTTTAACAGTCAGCTATCCCTATAGAACGAATAACATGATTATTTAATGGAACTTTTTACTTGATTAATACATTACAGTACAACAGGTTTTAGAGCATCATCCATCCTTTATCTACATTTGACGTAGCTATTCATACCTTTTCTTGATGTTCTGATTTAAAGGAAAAAAAATACCTAGCAAATGCAACTGCACAGCTCAGCATCTAAAGTACATTCTCTAACACGTAATTTGAACTAAATACAAGCTTGATCATTCTAGTTCTCTTATGATTACTGATGATTTATTTATGAATTTTTGCATAATTTGGTTTTAGTAATGAGGTTTATTTCAAGAAGCAAGGCTTTGGACATTCCGCATTATTGTTTTGAATATTATTTATTTGCATGGTAGCACTTTTTTTGTAATTTTCATCTCTCTCTCACATTTCAACTCTTCTGCTTCAGCCTGCAAAGAGACACGCATATGCTGGTGATAATGTATTTACTCCACTGGAGAGAGAGCTTGTTTTTGATATAGTAAGTAGATCGTTTTTTAAAATGTTCAAAATTCTAATTCAATTTGAAAGTAATTATAAGATCCTTTTCACTGAAAAATTAATCTGTAAAATTCCCCTATATGCAGGATATGACCGATTATGATGATGTTAGATATTGTTGTTCAGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGACTGCTGCTATCAAAGTGATTGATACTACCCTCAGAGGTAAGAAAATTCATCATCTGTATCTGAAAAGTTATTCAAGAAATGAATATGAATGCATGCATTCTCACCACTCTTAATTTGAAAAGGAATGAACAAAGAGAAGGTTGGATATCATTGAATGAAATCACTTACCTTCTTGGCAGTTTCCCCTCCCCAACCCAACTCCATAACCAAGCCTTTTTTTTTTTAATCTTTATTGGGAACGAGATGTTCCATATTGAGCCCTTAAGTTAACTTATTCCTAAATTCATACTTCAACCTATTGTTTTATTTGACTAGAAAAGAATTAGGAAGTAGAATATGTATGGAAGGTGGTATGCATTCTGAAATATTCATACTATGAATCACTACCTTATTGTCAGTTTTAAAAAATAATTACTAGCTTACTGTCAGTTTTACTTTTCTACTTTATCTCCTGCAGCCAGTTACGTATGCTGCTAAGTTTTATTTAAATATGTTCATACATTAAGCCTCTCACAACACTTTTTTGAAGGGATATTCAAAATTAACCATAATGGCACTGTGGCATTGTCAAATGCATCTGAGAATTAACTTTTCTATTGCAGTTCTATGATTAGATAGCGCCTGATGTTCTGTTGAGTCCCATTGGATACACTTCACTTGAACTTTGTATTGCAGATGACTTTGGATTTAACCACATATTGTGGGTATTTAGTGGTCGACGTGGTGTTCACTGTTGGGTCTGTGATGGCAAAGCAAGAAGGTAAAGCTTGTAATCTAATTGTTGAATTGTAGTCATGTATATTATTTGTAAAGGTCTAAAATTGTCACCATCACTGCTACAGGTTGTCTAATGAACAGAGAGCTGCCATTTCTGAATATTTCCATTTATATCAGGTTATACTACGTTGTTACTAAATATTGGTGAATTTTAGTGTCTTGAGCTATGGAGTTGAATGTAGTTGATGTGCAGGGTAACGAAAATAGTCGCAAGAAAATTTCACTAACGAGTCCAGTTCTTCATCCATTTCTTGCGTAAGTGGTGTTTAATATTAAGATATGTTGATTCAAATCTCATTTTTCTCTGCTATCTTATGTATTTTTTTTTCAGGAGATCATACATAGACGTTCTCGAGGATTGCTTTGAATCAAAATTGCTTTGTAGTCAAAAAATTTTCTCAACTGAGGAGAGATATGAGAAAATCTTAGATATGATTCATGATGAATGTAAGTGATTTATGTGTTTGTCTTAGGTAAGTACTGTCATTATGATAATCTGTACGTTAGATACTAATAGAGTTGCCCCTGTAGCCATTACCTCAGAGCTTCGTGGAAGGTGGCAAGAGAATAGGCGACCCTCTAAAAAAGATGTTAATTCCGTAAGATGGGAGCAACTAAAAAATATGTTGCAATCAGGGAAACATAAGGTACAATCAACATCTTTTCTCATACTTGCAAAAATTACAACTTGGAACAGTTAATTGAAGAGGGCCTTGTGTTGTAATATTGCTGTTGGGAACAATTTTTGTCTAGTGCAATGAGCACCAAAAATAGAGTTAGAAGTGATTTACTGTTTTTTTTCTTTTGTTTTTGCTTTCCTTTTTCGTACTCAAGTTACCAAACAGAGAAGGGATAACCATTTTACATGTTTTGTTTGTCTTTATGATGTACTCAAGTTTATGCGTTATTTGAAAGAAATGACAATCAGCCTAAAACATTACATAGAGGGGTTGTAGTTATGCATGAAAAGAAGGTGGGATAACCACTTATCAGCCTAACTAACCAACTAATCAGCCTAACAAATAAGACTTCAGCCGACTAATAGCCATAACAAACTAAAATAATATCACAAGAAGCAGCCAAAAGATTTACAAGAAAAGGGTAAAATAAAAATAACCACAAACGGTAAAATAGGCTAAGACATCCGAATGTCCCTACATCAAACCCCCACACTTTTAAGGGAACTTGTCCTTTTTTTTAGAAAGAGGAATACTTTAAGGGAACTTGTCCTTGAGTTTATGTGGCCAAAGAAAATTCATCAGGAGAAAAATATAGAACGATATCAGAAACGTAGAATGTGAAATTAATGTGCGTTGAAGAGGGAAGATCAATGTAATAGGCATTAGTGACAATACACTCCAAAATGGGAAGAGGGTCAATTGTCTTATTTGTGAGTTTGTTGTGAGTTCCTGAAGGTAGAATCGACTTGCGAAGGTGAACTATCACCAAGTGACCAATCTGAATTTCCTTGGCCCGTTGACAAGTATCAACATGGTCCTTATAACGTTGGTTAGCCTCTTCCAAATGAGTCTTGACTTCTTGATGCAAGGCTTGAATATGATCCACCATAGTTTCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAATATAGCATAGTTTCAGCTTTGAAGCTGAGATCAACCGAAGATAGAATGGATGTAAGATCAATAGTTAGTCTAGGTAAATGAGTATACACAACTTCAAATGGTGACTTCCGTGTGGAACGATTTTGCATATGATTGTAGGCAAATTCGACTTGAGGCAAAGATAGGTTCCATTACCTAGATTTCTCACCACTTAAGCAACAAATGAGATTACCAAGGGTGCAATTAGTGACTTTCATTTGCTCATCCGTCTGATGCTGACTAATAGTGCTCAATGGTAAACTTGTTCCAAAATGCTTCCACAAGGACTTCCAAAATAACTCATAATTTAACATCATGATTAGAAACTATGTTTTTAGGAACTCCATGAAGGCAAACAATTTCACAAAAGAAGAGATGAGCAATGTTTAGAGCATCGTCCAAAGTCTTTTTACAAGGGAGAAAATGGGCCATTTTGCTAAAATGATTGTTAGACCTCCTATGATTCACACTTATAAGAGAAAGGGTAAAAAGGGAGATACAGAGAAGGAGGAAGTTGGCATAAGTGGGGACTATTAGAGTGCAGAGTTCGTGGCTGGTATGATTGTCGGGTTGGAGGGGTATATTGTAGAGGGAAGAAGGGCAGACGGGATTGTCTGTTGATTTTGGGATTGCACCCTTGTCTTAG

mRNA sequence

ATGACGAGACCCACGACCATCCGCTTCAGATCTGCGGCTTCACGGCGAACGGACGACAGCTTCATGGCCATCCGTACTGTAGCGAAAATGGTTAACGAAGTGGTTGAGAATGGAGTAAGCGATATGGTAATTGATGGTAACGAACAAAAGCCGAAGAAAATTGTGCCTGATGGGTTCGATGCTAATTATCTCAAAATTTACTACGGGAAGCTTTTTCCGTATACTGATTTTTTCAAATGGATGTCTTATGGAAATGATGGAAAGCATCCAGGCAGTGACCGATCCTATTTTGGCCGAAGAGAGTTCTCATTCACGTTGGATAATGATATTTATCTACGATTCCAATCTTTCAACAGTTTGTCTGAAATGGAAAATTCGATCAAGGAAAAGTGTCCGTTTAAGATTGATATTGGGTCTGTATACAGCGTGGATCCTGCAAAGAGACACGCATATGCTGGTGATAATGTATTTACTCCACTGGAGAGAGAGCTTGTTTTTGATATAGATATGACCGATTATGATGATGTTAGATATTGTTGTTCAGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGACTGCTGCTATCAAAGTGATTGATACTACCCTCAGAGATGACTTTGGATTTAACCACATATTGTGGGTATTTAGTGGTCGACGTGGTGTTCACTGTTGGGTCTGTGATGGCAAAGCAAGAAGGTTGTCTAATGAACAGAGAGCTGCCATTTCTGAATATTTCCATTTATATCAGGGTAACGAAAATAGTCGCAAGAAAATTTCACTAACGAGTCCAGTTCTTCATCCATTTCTTGCGAGATCATACATAGACGTTCTCGAGGATTGCTTTGAATCAAAATTGCTTTGTAGTCAAAAAATTTTCTCAACTGAGGAGAGATATGAGAAAATCTTAGATATGATTCATGATGAATATACTAATAGAGTTGCCCCTGTAGCCATTACCTCAGAGCTTCGTGGAAGGTGGCAAGAGAATAGGCGACCCTCTAAAAAAGATGTTAATTCCGTAAGATGGGAGCAACTAAAAAATATGTTGCAATCAGGGAAACATAAGAGGGAAGAAGGGCAGACGGGATTGTCTGTTGATTTTGGGATTGCACCCTTGTCTTAG

Coding sequence (CDS)

ATGACGAGACCCACGACCATCCGCTTCAGATCTGCGGCTTCACGGCGAACGGACGACAGCTTCATGGCCATCCGTACTGTAGCGAAAATGGTTAACGAAGTGGTTGAGAATGGAGTAAGCGATATGGTAATTGATGGTAACGAACAAAAGCCGAAGAAAATTGTGCCTGATGGGTTCGATGCTAATTATCTCAAAATTTACTACGGGAAGCTTTTTCCGTATACTGATTTTTTCAAATGGATGTCTTATGGAAATGATGGAAAGCATCCAGGCAGTGACCGATCCTATTTTGGCCGAAGAGAGTTCTCATTCACGTTGGATAATGATATTTATCTACGATTCCAATCTTTCAACAGTTTGTCTGAAATGGAAAATTCGATCAAGGAAAAGTGTCCGTTTAAGATTGATATTGGGTCTGTATACAGCGTGGATCCTGCAAAGAGACACGCATATGCTGGTGATAATGTATTTACTCCACTGGAGAGAGAGCTTGTTTTTGATATAGATATGACCGATTATGATGATGTTAGATATTGTTGTTCAGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGACTGCTGCTATCAAAGTGATTGATACTACCCTCAGAGATGACTTTGGATTTAACCACATATTGTGGGTATTTAGTGGTCGACGTGGTGTTCACTGTTGGGTCTGTGATGGCAAAGCAAGAAGGTTGTCTAATGAACAGAGAGCTGCCATTTCTGAATATTTCCATTTATATCAGGGTAACGAAAATAGTCGCAAGAAAATTTCACTAACGAGTCCAGTTCTTCATCCATTTCTTGCGAGATCATACATAGACGTTCTCGAGGATTGCTTTGAATCAAAATTGCTTTGTAGTCAAAAAATTTTCTCAACTGAGGAGAGATATGAGAAAATCTTAGATATGATTCATGATGAATATACTAATAGAGTTGCCCCTGTAGCCATTACCTCAGAGCTTCGTGGAAGGTGGCAAGAGAATAGGCGACCCTCTAAAAAAGATGTTAATTCCGTAAGATGGGAGCAACTAAAAAATATGTTGCAATCAGGGAAACATAAGAGGGAAGAAGGGCAGACGGGATTGTCTGTTGATTTTGGGATTGCACCCTTGTCTTAG

Protein sequence

MTRPTTIRFRSAASRRTDDSFMAIRTVAKMVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQENRRPSKKDVNSVRWEQLKNMLQSGKHKREEGQTGLSVDFGIAPLS

Homology

[Close]

BLAST of Spg004428 vs. NCBI nr
Match: XP_022925368.1 (DNA primase small subunit [Cucurbita moschata])

HSP 1 Score: 612.5 bits (1578), Expect = 2.5e-171
Identity = 293/328 (89.33%), Postives = 305/328 (92.99%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           MV+E  ENG SDMVIDGNEQKPK IV +GFDANYL+IYYGKLFP+ DFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLEIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIG VYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYAG NVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI++YFHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FLARSYI+VL+D FESKLLCSQKIFSTEERYEKILDMIHDE        +ITSELRGRWQ
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDE--------SITSELRGRWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHK 358
           +NRRPSK DVN VRWEQLKNMLQSGKHK
Sbjct: 301 DNRRPSKNDVNLVRWEQLKNMLQSGKHK 320

[Close]

BLAST of Spg004428 vs. NCBI nr
Match: KAG6592333.1 (DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.5 bits (1578), Expect = 2.5e-171
Identity = 292/330 (88.48%), Postives = 306/330 (92.73%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           MV+E  ENG SDMVIDGNEQKPK IV +GFDANYLKIYYGKLFP+ DFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLKIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIG VYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYAG NVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVID+TLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDSTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI++YFHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FLARSYI+VL+D FESKLLCSQKIFSTEERYEK+LDMIHDE        +ITSELRGRWQ
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKVLDMIHDE--------SITSELRGRWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           +NRRPSK DVN VRWEQLKNMLQSGKHK +
Sbjct: 301 DNRRPSKNDVNLVRWEQLKNMLQSGKHKSQ 322

[Close]

BLAST of Spg004428 vs. NCBI nr
Match: KAA0039338.1 (DNA primase, small subunit [Cucumis melo var. makuwa] >TYK00521.1 DNA primase, small subunit [Cucumis melo var. makuwa])

HSP 1 Score: 612.1 bits (1577), Expect = 3.3e-171
Identity = 294/333 (88.29%), Postives = 308/333 (92.49%), Query Frame = 0


Query: 27  VAKMVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGND 86
           VAKMV   VENG SDMVIDGNEQKPKK V DGFDANYLKIYYGKLFPY DFFKWMSYGND
Sbjct: 13  VAKMVTAAVENGGSDMVIDGNEQKPKKSVSDGFDANYLKIYYGKLFPYADFFKWMSYGND 72

Query: 87  GKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPA 146
           GKHPGSD+SYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKE+CPFKIDIG VYSVDPA
Sbjct: 73  GKHPGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEQCPFKIDIGPVYSVDPA 132

Query: 147 KRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRD 206
           KRHAYAGDNVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRD
Sbjct: 133 KRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRD 192

Query: 207 DFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPV 266
           DFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI+EYFHLYQGNENSRKKISLT PV
Sbjct: 193 DFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPV 252

Query: 267 LHPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRG 326
           LHPFLARSY +VL+D FES+LLC QKIFSTEERYEKILDMI DE        +ITS+LRG
Sbjct: 253 LHPFLARSYTEVLKDSFESQLLCRQKIFSTEERYEKILDMIPDE--------SITSDLRG 312

Query: 327 RWQENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           RWQ+NRR SK DVNS+RWEQLKN+LQSGKHK +
Sbjct: 313 RWQDNRRSSKNDVNSLRWEQLKNLLQSGKHKAQ 337

[Close]

BLAST of Spg004428 vs. NCBI nr
Match: XP_023535939.1 (DNA primase small subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.9 bits (1574), Expect = 7.3e-171
Identity = 293/330 (88.79%), Postives = 305/330 (92.42%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           MV+E  ENG SDMVIDGNEQKPK IV + FDANYLKIYYGKLFP+ DFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNVFDANYLKIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIG VYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYAG NVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI++YFHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FLARSYI+VL+D FESKLLCSQKIFSTEERYEKILDMIHDE        +ITSELRGRWQ
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDE--------SITSELRGRWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           +NRRPSK DVN VRWEQLKNMLQSGKHK +
Sbjct: 301 DNRRPSKNDVNLVRWEQLKNMLQSGKHKSQ 322

[Close]

BLAST of Spg004428 vs. NCBI nr
Match: XP_038889665.1 (DNA primase small subunit [Benincasa hispida])

HSP 1 Score: 609.4 bits (1570), Expect = 2.1e-170
Identity = 291/330 (88.18%), Postives = 306/330 (92.73%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           MV   +ENG SDMVIDGNEQKPK  V DGFDANYLKIYYGKLFPY DFFKWMSYGNDGKH
Sbjct: 1   MVTAAIENGESDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PG+D+SYFGRREFSFTLDND+YLRFQSFNSLSEMENSIKEKCPFKIDIG VYSVDPAKRH
Sbjct: 61  PGNDQSYFGRREFSFTLDNDVYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYAGDNVFTP+ERELVFDIDMTDYDDVRYCCSGA VCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGAGVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI+EYFHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FLARSYI+VL+D FES+LL SQKIFSTEERYEKILDMIHDE        +ITSELRG+WQ
Sbjct: 241 FLARSYIEVLKDSFESQLLGSQKIFSTEERYEKILDMIHDE--------SITSELRGKWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           +NRR SK DVNSVRWE+LKNMLQSGK+K +
Sbjct: 301 DNRRTSKNDVNSVRWEKLKNMLQSGKYKAQ 322

[Close]

BLAST of Spg004428 vs. ExPASy Swiss-Prot
Match: P20664 (DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.9e-69
Identity = 133/297 (44.78%), Postives = 189/297 (63.64%), Query Frame = 0


Query: 64  LKIYYGKLFPYTDFFKWMSYGNDGKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEM 123
           LK+YY +LFPY  +++W++YG      G  ++YF  REFSFTL +DIY+R+QSFN+ SE+
Sbjct: 13  LKLYYRRLFPYAQYYRWLNYG------GVTKNYFQHREFSFTLKDDIYIRYQSFNNQSEL 72

Query: 124 ENSIKEKCPFKIDIGSVYSVDPAKRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGA 183
           E  +++  P+KIDIG+VYS  P  +H       F   E+ELVFDIDMTDYDDVR CCS A
Sbjct: 73  EKEMQKMNPYKIDIGAVYSHRP-NQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSA 132

Query: 184 DVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI 243
           D+C  CW LMT A+++ID  L++DFGF H LWV+SGRRGVHCWVCD   R+LS+  R+ I
Sbjct: 133 DICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGI 192

Query: 244 SEYFHLYQGNENSRKKISLTSPVLHPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKI 303
            EY  L +G ++ +KK+ L   V HPF+ +S I++++  FE   L  Q I   +E ++KI
Sbjct: 193 VEYLSLVKGGQDVKKKVHLNEKV-HPFVRKS-INIIKKYFEEYALVGQDILENKENWDKI 252

Query: 304 LDMIHDEYTNRVAPVAITSELRGRWQENRRPSKKDVNSVRWEQLKNMLQSGKHKREE 361
           L ++         P  I  EL+  +Q      K   +  RWE L+ +  S ++ + +
Sbjct: 253 LALV---------PETIHDELQRGFQ------KFHSSPQRWEHLRKVANSSQNMKND 285

[Close]

BLAST of Spg004428 vs. ExPASy Swiss-Prot
Match: P49642 (DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 6.4e-69
Identity = 135/288 (46.88%), Postives = 189/288 (65.62%), Query Frame = 0


Query: 64  LKIYYGKLFPYTDFFKWMSYGNDGKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEM 123
           LK+YY +LFPY+ +++W++YG      G  ++YF  REFSFTL +DIY+R+QSFN+ S++
Sbjct: 13  LKLYYRRLFPYSQYYRWLNYG------GVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDL 72

Query: 124 ENSIKEKCPFKIDIGSVYSVDPAKRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGA 183
           E  +++  P+KIDIG+VYS  P  +H       F   E+ELVFDIDMTDYDDVR CCS A
Sbjct: 73  EKEMQKMNPYKIDIGAVYSHRP-NQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSA 132

Query: 184 DVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI 243
           D+C  CW LMT AI++ID  L++DFGF H LWV+SGRRGVHCWVCD   R+LS+  R+ I
Sbjct: 133 DICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGI 192

Query: 244 SEYFHLYQGNENSRKKISLTSPVLHPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKI 303
            EY  L +G ++ +KK+ L S  +HPF+ +S I++++  FE   L +Q I   +E ++KI
Sbjct: 193 VEYLSLVKGGQDVKKKVHL-SEKIHPFIRKS-INIIKKYFEEYALVNQDILENKESWDKI 252

Query: 304 LDMIHDEYTNRVAPVAITSELRGRWQENRRPSKKDVNSV-RWEQLKNM 351
           L ++         P  I  EL+  +Q       K  NS+ RWE LK +
Sbjct: 253 LALV---------PETIHDELQQSFQ-------KSHNSLQRWEHLKKV 275

[Close]

BLAST of Spg004428 vs. ExPASy Swiss-Prot
Match: Q24317 (DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-58
Identity = 118/273 (43.22%), Postives = 165/273 (60.44%), Query Frame = 0


Query: 64  LKIYYGKLFPYTDFFKWMSYGNDGKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEM 123
           L +YY +LFP+  F++W+SYG+      S+ + F  RE SFTL +DIY+R+  F++ +E+
Sbjct: 32  LPVYYRRLFPHLPFYRWLSYGS------SEDAIFSNREISFTLQDDIYIRYLCFDTQAEL 91

Query: 124 ENSIKEKCPFKIDIGSVYSVDPAKRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGA 183
           E  I  + P KIDIG V    P    +  G    TP++RELVFDIDMTDYD+VR CCSGA
Sbjct: 92  EKEICSRNPIKIDIGPVMHSKPKNHRSIPGG--LTPVQRELVFDIDMTDYDEVRTCCSGA 151

Query: 184 DVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI 243
            VCL CW  M  A +V+D  LR+DFGF HI+W+FSGRRG+HCWVCD +AR L    R A+
Sbjct: 152 GVCLKCWKFMVLAARVLDVALREDFGFEHIIWIFSGRRGIHCWVCDYQARHLDGRGRYAV 211

Query: 244 SEYFHL-----YQGNENSRKKISLTSPVLHPFLARSYIDVLEDCFESKLLCSQKIFSTEE 303
           +EY ++     + G  + R  +    P  H  L R+ + ++E  FE  +L  Q +F T +
Sbjct: 212 AEYLNIITYASFAGGNSPRCSMG-DRP--HHSLKRA-LKIVEPMFEEIVLEDQNLFGTPK 271

Query: 304 RYEKILDMIHDEYTNRVAPVAITSELRGRWQEN 332
              K+L+M+HD+        A   EL    Q+N
Sbjct: 272 GVTKLLNMVHDD--------AARGELESYMQKN 284

[Close]

BLAST of Spg004428 vs. ExPASy Swiss-Prot
Match: O14215 (DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pri1 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.4e-54
Identity = 119/331 (35.95%), Postives = 183/331 (55.29%), Query Frame = 0


Query: 29  KMVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYG---- 88
           K ++E++ NG     +DG +Q          D+  +  YY  LFP+   F+W+++G    
Sbjct: 11  KDLDEIIANG----TLDGAKQ-------GAVDSETMIQYYRHLFPWKYLFQWLNHGPVVT 70

Query: 89  NDGKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVD 148
           ND          F  REF+FTL ND Y+R+ SF++  E++      CP + ++G VYS +
Sbjct: 71  ND----------FAHREFAFTLPNDAYIRYLSFSNWEELKKEALNLCPSRFEVGPVYSAN 130

Query: 149 PAKRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTL 208
           P  R      + F PL++ELVFDIDMTDYDDVR CCS  ++C  CWP +T A++V+D   
Sbjct: 131 PRDRKTIR-KSTFHPLKKELVFDIDMTDYDDVRTCCSKTNICEKCWPFITIAVQVLDICF 190

Query: 209 RDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGN-ENSRKKISLT 268
            +DFGF HILWV+SGRRG+H W+CD  A  L +  R  I+ Y  +  GN +   + I+  
Sbjct: 191 HEDFGFKHILWVYSGRRGIHAWICDEIACSLDDRSRRMIASYLQVVVGNPQGGVRLINNL 250

Query: 269 SPVLHPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSE 328
              LHP L RS +++L+  F   +L  Q  ++++E  E +L ++ D+         + S 
Sbjct: 251 KRPLHPHLTRS-LNILKSAFVKIVLEDQDPWASKEGAENLLKLLPDK--------DLASA 306

Query: 329 LRGRWQENRRPSKKDVNSVRWEQLKNMLQSG 355
           LR +W+ +   S K+    +W  +  +L SG
Sbjct: 311 LRKKWEVDPERSSKN----KWSDIDTVLASG 306

[Close]

BLAST of Spg004428 vs. ExPASy Swiss-Prot
Match: Q7KQM1 (DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF14_0366 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.3e-49
Identity = 97/234 (41.45%), Postives = 143/234 (61.11%), Query Frame = 0


Query: 62  NYLKIYYGKLFPYTDFFKWMSYGND--GKHPG-SDRSYFGRREFSFTL------DNDIYL 121
           N L  YY  L P  D + W++Y ND  GK+   +D  +F +REFSFT         +IY+
Sbjct: 17  NDLIFYYRSLCPINDLYNWLNYKNDIKGKYTKLNDPHFFSKREFSFTCKKSDQGKEEIYI 76

Query: 122 RFQSFNSLSEMENS-IKEKCPFKIDIGSVYSVDPAKRHAYAGDNVFTPLERELVFDIDMT 181
           R+ SF++  E +N  + +  P K DIG++Y+  P  +    GD +F P+++EL+FDIDM 
Sbjct: 77  RWLSFSNPEEFKNKLLSDLVPIKFDIGAIYNF-PVSQKDQKGD-IFLPVQKELIFDIDMN 136

Query: 182 DYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGK 241
           DYDD+R CC+   VC  CW  +T AI ++DT LR+DF F HILWV+SGRRG+HCWV D  
Sbjct: 137 DYDDIRTCCTDKKVCKLCWKFLTVAIVLLDTALREDFSFEHILWVYSGRRGIHCWVADES 196

Query: 242 ARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHPFLARS------YIDVL 280
            R  + + RAA+++Y ++  G++  +KK+S+     +P   R+      Y DVL
Sbjct: 197 CRYYTTDARAALADYLNILSGSDTKKKKVSIWGKDKYPMFERAFDICYKYFDVL 248

[Close]

BLAST of Spg004428 vs. ExPASy TrEMBL
Match: A0A6J1EEZ9 (DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 293/328 (89.33%), Postives = 305/328 (92.99%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           MV+E  ENG SDMVIDGNEQKPK IV +GFDANYL+IYYGKLFP+ DFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLEIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIG VYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYAG NVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI++YFHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FLARSYI+VL+D FESKLLCSQKIFSTEERYEKILDMIHDE        +ITSELRGRWQ
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDE--------SITSELRGRWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHK 358
           +NRRPSK DVN VRWEQLKNMLQSGKHK
Sbjct: 301 DNRRPSKNDVNLVRWEQLKNMLQSGKHK 320

[Close]

BLAST of Spg004428 vs. ExPASy TrEMBL
Match: A0A5A7T8Y3 (DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.6e-171
Identity = 294/333 (88.29%), Postives = 308/333 (92.49%), Query Frame = 0


Query: 27  VAKMVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGND 86
           VAKMV   VENG SDMVIDGNEQKPKK V DGFDANYLKIYYGKLFPY DFFKWMSYGND
Sbjct: 13  VAKMVTAAVENGGSDMVIDGNEQKPKKSVSDGFDANYLKIYYGKLFPYADFFKWMSYGND 72

Query: 87  GKHPGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPA 146
           GKHPGSD+SYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKE+CPFKIDIG VYSVDPA
Sbjct: 73  GKHPGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEQCPFKIDIGPVYSVDPA 132

Query: 147 KRHAYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRD 206
           KRHAYAGDNVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRD
Sbjct: 133 KRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRD 192

Query: 207 DFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPV 266
           DFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI+EYFHLYQGNENSRKKISLT PV
Sbjct: 193 DFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPV 252

Query: 267 LHPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRG 326
           LHPFLARSY +VL+D FES+LLC QKIFSTEERYEKILDMI DE        +ITS+LRG
Sbjct: 253 LHPFLARSYTEVLKDSFESQLLCRQKIFSTEERYEKILDMIPDE--------SITSDLRG 312

Query: 327 RWQENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           RWQ+NRR SK DVNS+RWEQLKN+LQSGKHK +
Sbjct: 313 RWQDNRRSSKNDVNSLRWEQLKNLLQSGKHKAQ 337

[Close]

BLAST of Spg004428 vs. ExPASy TrEMBL
Match: A0A6J1CKZ3 (DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 7.4e-169
Identity = 287/330 (86.97%), Postives = 305/330 (92.42%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           M+ E VENG  DM IDG EQKP+K+VPDGFD NYLKIYYGKLFPYTDFFKWMSYGNDGKH
Sbjct: 1   MITEAVENGGGDMEIDGFEQKPEKVVPDGFDVNYLKIYYGKLFPYTDFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PGSD+SYFGRREFSFTL+NDIYLR+QSFN+LSEMENSIKEKCPFKIDIG VYSVD AKRH
Sbjct: 61  PGSDQSYFGRREFSFTLENDIYLRYQSFNNLSEMENSIKEKCPFKIDIGPVYSVDLAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYAGDNVFTP+EREL+FDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGDNVFTPVERELIFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI+E+FHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEHFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FLARSY +VL+D FES LLCSQKIFSTEERYEKILDMI DE        ++TSELRGRWQ
Sbjct: 241 FLARSYTEVLKDSFESTLLCSQKIFSTEERYEKILDMIPDE--------SVTSELRGRWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           ENR+PSKKDVN VRWEQLK+MLQS KHK +
Sbjct: 301 ENRQPSKKDVNYVRWEQLKSMLQSVKHKAQ 322

[Close]

BLAST of Spg004428 vs. ExPASy TrEMBL
Match: A0A6J1I8T4 (DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 287/320 (89.69%), Postives = 299/320 (93.44%), Query Frame = 0


Query: 40  SDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKHPGSDRSYFGR 99
           +DMVIDGNEQKPK IV +GFDANYLKIYYGKLFP+ DFFKWMSYGNDGKHPGSD SY GR
Sbjct: 6   ADMVIDGNEQKPKNIVSNGFDANYLKIYYGKLFPFADFFKWMSYGNDGKHPGSDHSYVGR 65

Query: 100 REFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRHAYAGDNVFTP 159
           REFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIG VYSVDPAKRHAYAG NVFTP
Sbjct: 66  REFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRHAYAGANVFTP 125

Query: 160 LERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG 219
           +ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG
Sbjct: 126 VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG 185

Query: 220 RRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHPFLARSYIDVL 279
           RRGVHCWVCDGKARRLSNEQRAAI++YFHLYQGNENSRKKISLT PVLHPFLARSYI+VL
Sbjct: 186 RRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVL 245

Query: 280 EDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQENRRPSKKDV 339
           +D FESKLLCSQKIFSTEERYEKILDMIHDE        +ITSELRGRWQ+NRRPSK DV
Sbjct: 246 KDSFESKLLCSQKIFSTEERYEKILDMIHDE--------SITSELRGRWQDNRRPSKNDV 305

Query: 340 NSVRWEQLKNMLQSGKHKRE 360
           N VRWEQLKNMLQSGKHK +
Sbjct: 306 NLVRWEQLKNMLQSGKHKSQ 317

[Close]

BLAST of Spg004428 vs. ExPASy TrEMBL
Match: A0A0A0KUP7 (DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.8e-168
Identity = 288/330 (87.27%), Postives = 302/330 (91.52%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           MV   VENG SDMVIDGNEQKPK  V DGFDANYLKIYYGKLFPY DFFKWMSYGNDGKH
Sbjct: 1   MVTAAVENGGSDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PGSD+SYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKEKCPFKIDIG VYSVDPAKRH
Sbjct: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 150 AYAGDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 209
           AYA DNVFTP+ERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVI TTLRDDFG
Sbjct: 121 AYADDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIHTTLRDDFG 180

Query: 210 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVLHP 269
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI+EYFHLYQGNENSRKKISLT PVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 270 FLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGRWQ 329
           FL RSY +VL+D FES+LLCSQKIFSTEERYEKILDMI DE        +ITS+LRGRWQ
Sbjct: 241 FLVRSYTEVLKDSFESQLLCSQKIFSTEERYEKILDMIPDE--------SITSDLRGRWQ 300

Query: 330 ENRRPSKKDVNSVRWEQLKNMLQSGKHKRE 360
           +NRR SK DVNS+RWEQLK++LQSGKHK +
Sbjct: 301 DNRRSSKNDVNSLRWEQLKSLLQSGKHKAQ 322

[Close]

BLAST of Spg004428 vs. TAIR 10
Match: AT5G41880.1 (DNA primases;DNA primases )

HSP 1 Score: 431.0 bits (1107), Expect = 9.6e-121
Identity = 207/332 (62.35%), Postives = 255/332 (76.81%), Query Frame = 0


Query: 30  MVNEVVENGVSDMVIDGNEQKPKKIVPDGFDANYLKIYYGKLFPYTDFFKWMSYGNDGKH 89
           M  E  E    DM+ID     PK  + + F+ +YL+IYYG LFPYTD  KW+SYG+DGKH
Sbjct: 1   MTREETETSGEDMMID----DPKPKIENDFNVHYLRIYYGNLFPYTDIHKWLSYGHDGKH 60

Query: 90  PGSDRSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGSVYSVDPAKRH 149
           PG D  YFGRREFSFTL+ND+YLR++SF + S ME +IK   P+KIDIG+VYSVDP KRH
Sbjct: 61  PGCDEYYFGRREFSFTLENDVYLRYKSFKNASLMEAAIKSNFPYKIDIGAVYSVDPDKRH 120

Query: 150 AYA--GDNVFTPLERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDD 209
           AYA  G N+FTP+ERELVFDID+TDYDDVRYCCSGADVC  CWPLMT AIKVIDT+LR+D
Sbjct: 121 AYAQSGTNLFTPVERELVFDIDITDYDDVRYCCSGADVCSKCWPLMTVAIKVIDTSLRED 180

Query: 210 FGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAISEYFHLYQGNENSRKKISLTSPVL 269
           FGF HILWVFSGRRGVHCWVCD KARRL+NEQR+A++EYF +Y+GNEN+ KK+ L    L
Sbjct: 181 FGFKHILWVFSGRRGVHCWVCDAKARRLTNEQRSAVAEYFRVYKGNENNAKKVDLMGHSL 240

Query: 270 HPFLARSYIDVLEDCFESKLLCSQKIFSTEERYEKILDMIHDEYTNRVAPVAITSELRGR 329
           HPFLARSY+D ++  FE +L  +Q IFS++E+YEKIL MI DE         I ++LRG+
Sbjct: 241 HPFLARSYVDFIKSFFEGELQATQSIFSSKEKYEKILGMITDE--------DIQADLRGK 300

Query: 330 WQENRRP--SKKDVNSVRWEQLKNMLQSGKHK 358
           W+ + R   S++  + +RWEQLK  LQS ++K
Sbjct: 301 WENSARSSLSEEATSLLRWEQLKKALQSKRNK 320

The following BLAST results are available for this feature:

BLAST of Spg004428 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925368.1	2.5e-171	89.33	DNA primase small subunit [Cucurbita moschata]	[more]
KAG6592333.1	2.5e-171	88.48	DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAA0039338.1	3.3e-171	88.29	DNA primase, small subunit [Cucumis melo var. makuwa] >TYK00521.1 DNA primase, s...	[more]
XP_023535939.1	7.3e-171	88.79	DNA primase small subunit [Cucurbita pepo subsp. pepo]	[more]
XP_038889665.1	2.1e-170	88.18	DNA primase small subunit [Benincasa hispida]	[more]

BLAST of Spg004428 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P20664	4.9e-69	44.78	DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1	[more]
P49642	6.4e-69	46.88	DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1	[more]
Q24317	1.3e-58	43.22	DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2	[more]
O14215	3.4e-54	35.95	DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)...	[more]
Q7KQM1	4.3e-49	41.45	DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF1...	[more]

BLAST of Spg004428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EEZ9	1.2e-171	89.33	DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1	[more]
A0A5A7T8Y3	1.6e-171	88.29	DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 P...	[more]
A0A6J1CKZ3	7.4e-169	86.97	DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1	[more]
A0A6J1I8T4	2.1e-168	89.69	DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1	[more]
A0A0A0KUP7	4.8e-168	87.27	DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1	[more]

BLAST of Spg004428 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41880.1	9.6e-121	62.35	DNA primases;DNA primases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.920.10	DNA primase, PRIM domain	coord: 53..369 e-value: 6.1E-106 score: 356.7
None	No IPR available	PANTHER	PTHR10536:SF1	DNA PRIMASE	coord: 70..358
None	No IPR available	SUPERFAMILY	56747	Prim-pol domain	coord: 61..318
IPR002755	DNA primase, small subunit	PFAM	PF01896	DNA_primase_S	coord: 166..342 e-value: 6.5E-43 score: 146.9
IPR014052	DNA primase, small subunit, eukaryotic/archaeal	TIGRFAM	TIGR00335	TIGR00335	coord: 65..351 e-value: 1.2E-45 score: 154.2
IPR014052	DNA primase, small subunit, eukaryotic/archaeal	PANTHER	PTHR10536	DNA PRIMASE SMALL SUBUNIT	coord: 70..358
IPR014052	DNA primase, small subunit, eukaryotic/archaeal	CDD	cd04860	AE_Prim_S	coord: 73..278 e-value: 1.10782E-80 score: 244.831

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg004428.1	Spg004428.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006269	DNA replication, synthesis of RNA primer
cellular_component	GO:0005658	alpha DNA polymerase:primase complex
molecular_function	GO:0003896	DNA primase activity
molecular_function	GO:0046872	metal ion binding