Spg004265 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004265
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionserine/threonine-protein kinase WAG1-like
Locationscaffold6: 6734005 .. 6735429 (-)
RNA-Seq ExpressionSpg004265
SyntenySpg004265
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATGGACGACGATTTCTCCATCTTCCCTCCTGATTCAGATCTCGACTTCAGCTTCACCTCCACCTCCACCGCCACCGACCGCACCTTCGCCTCCTCCAGCGCCCGCAGCAGTCTCGCCAGAACCAGCCTTACTCTCAGCTTCAACGAATCCCGTCTCTCCGCCGCTGCTGCCGCCCTCAACCAACACCCCCACCGTCACTCCGACCCGCACTGGTCCGCCATCAAAGCCGCGACCACTCTCTCTTCCGACGGCCACCTCCACCTCCGCCACCTGAAACTCATCCGCCACCTCGGCACCGGCAACCTCGGCCGCGTCTTCCTCTGCCATTTGAGGGACAACGACCACGCCAGTTTCGCTCTCAAGGTCGTCGACAGGGAGGCTTTGAGCAACAAGAAGCTTTTGCAGGTTCAAACGGAAGCCGAGATTCTCGCCTTGCTGGACCACCCTTTTCTCCCCACTCTCTACACGCGTCTCGACGTCTCTCACTACACGTGCCTCCTCATCGACTACTGCCCCGCCGGCGACCTCCACTCCCTCCTCCGCAAACAGCCCGGAAACCGACTCTCCGTCTCGGCGGCCAGATTCTTCGCCGCCGAGGTCCTGGTGGCGCTCGAGTACCTTCACGCGCTCGGGATCGTCTACCGGGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCTCTGATTTCGACTTGTGCTTTAAGTCCGACGTGGTCCCCACTTTTCACACGTGGACGCGGCGGGGACCACAGGGCAGTGGCTGTTTTGGGTTGAGGACGGCGGCGGCGGCGGAGGAGGAGATTGTGGGGGAGTTTGTGGCGGAGCCGACGGCGGCGTTTTCGAAGTCGTGTGTGGGGACTCATGAGTATTTGGCGCCGGAGTTGGTGGCCGGCGGTGGCCACGGCAACGGCGTCGACTGGTGGGCGTTTGGGGTGTTCGTTTACGAGCTCCTGCACGGAACGACGCCGTTTAAAGGAGTCAACAAGGAGGGAACTCTAAGAAACATTGCGTTGTCGGACGGGGTGAAGTTCCGGGCTGTGGCGGCGGCGGAGGAAGAGGAGGGGATGGCGGAGGCGAGGGATCTGATAGAGCGGCTGCTGGTGAAGGATCCGGCTCGGCGGATGGGGTCCGGCAAAGGGGCGACTGAGATCAAACGGCATCCGTTCTTCGACGGGATAAAATGGCCGTTGATCCGGACGTACCGGGCGCCGGAGGTGTATGGGCTGATGAGAAAAGCCAGATCGCACGTGAGCCACGTGACTGGCGTGACCCACCGCATGAGGCGGCGGGGGTGGTGGAACTGGAAGAAGCTGAGTAACCGTTTAATGAAAAATGGAAGTTCCAGTAAATCTCATACCAATACCAATTATTACAGTTATAGCGCCAAAAATAAGAAGGGTTAA

mRNA sequence

ATGGATATGGACGACGATTTCTCCATCTTCCCTCCTGATTCAGATCTCGACTTCAGCTTCACCTCCACCTCCACCGCCACCGACCGCACCTTCGCCTCCTCCAGCGCCCGCAGCAGTCTCGCCAGAACCAGCCTTACTCTCAGCTTCAACGAATCCCGTCTCTCCGCCGCTGCTGCCGCCCTCAACCAACACCCCCACCGTCACTCCGACCCGCACTGGTCCGCCATCAAAGCCGCGACCACTCTCTCTTCCGACGGCCACCTCCACCTCCGCCACCTGAAACTCATCCGCCACCTCGGCACCGGCAACCTCGGCCGCGTCTTCCTCTGCCATTTGAGGGACAACGACCACGCCAGTTTCGCTCTCAAGGTCGTCGACAGGGAGGCTTTGAGCAACAAGAAGCTTTTGCAGGTTCAAACGGAAGCCGAGATTCTCGCCTTGCTGGACCACCCTTTTCTCCCCACTCTCTACACGCGTCTCGACGTCTCTCACTACACGTGCCTCCTCATCGACTACTGCCCCGCCGGCGACCTCCACTCCCTCCTCCGCAAACAGCCCGGAAACCGACTCTCCGTCTCGGCGGCCAGATTCTTCGCCGCCGAGGTCCTGGTGGCGCTCGAGTACCTTCACGCGCTCGGGATCGTCTACCGGGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCTCTGATTTCGACTTGTGCTTTAAGTCCGACGTGGTCCCCACTTTTCACACGTGGACGCGGCGGGGACCACAGGGCAGTGGCTGTTTTGGGTTGAGGACGGCGGCGGCGGCGGAGGAGGAGATTGTGGGGGAGTTTGTGGCGGAGCCGACGGCGGCGTTTTCGAAGTCGTGTGTGGGGACTCATGAGTATTTGGCGCCGGAGTTGGTGGCCGGCGGTGGCCACGGCAACGGCGTCGACTGGTGGGCGTTTGGGGTGTTCGTTTACGAGCTCCTGCACGGAACGACGCCGTTTAAAGGAGTCAACAAGGAGGGAACTCTAAGAAACATTGCGTTGTCGGACGGGGTGAAGTTCCGGGCTGTGGCGGCGGCGGAGGAAGAGGAGGGGATGGCGGAGGCGAGGGATCTGATAGAGCGGCTGCTGGTGAAGGATCCGGCTCGGCGGATGGGGTCCGGCAAAGGGGCGACTGAGATCAAACGGCATCCGTTCTTCGACGGGATAAAATGGCCGTTGATCCGGACGTACCGGGCGCCGGAGGTGTATGGGCTGATGAGAAAAGCCAGATCGCACGTGAGCCACGTGACTGGCGTGACCCACCGCATGAGGCGGCGGGGGTGGTGGAACTGGAAGAAGCTGAGTAACCGTTTAATGAAAAATGGAAGTTCCAGTAAATCTCATACCAATACCAATTATTACAGTTATAGCGCCAAAAATAAGAAGGGTTAA

Coding sequence (CDS)

ATGGATATGGACGACGATTTCTCCATCTTCCCTCCTGATTCAGATCTCGACTTCAGCTTCACCTCCACCTCCACCGCCACCGACCGCACCTTCGCCTCCTCCAGCGCCCGCAGCAGTCTCGCCAGAACCAGCCTTACTCTCAGCTTCAACGAATCCCGTCTCTCCGCCGCTGCTGCCGCCCTCAACCAACACCCCCACCGTCACTCCGACCCGCACTGGTCCGCCATCAAAGCCGCGACCACTCTCTCTTCCGACGGCCACCTCCACCTCCGCCACCTGAAACTCATCCGCCACCTCGGCACCGGCAACCTCGGCCGCGTCTTCCTCTGCCATTTGAGGGACAACGACCACGCCAGTTTCGCTCTCAAGGTCGTCGACAGGGAGGCTTTGAGCAACAAGAAGCTTTTGCAGGTTCAAACGGAAGCCGAGATTCTCGCCTTGCTGGACCACCCTTTTCTCCCCACTCTCTACACGCGTCTCGACGTCTCTCACTACACGTGCCTCCTCATCGACTACTGCCCCGCCGGCGACCTCCACTCCCTCCTCCGCAAACAGCCCGGAAACCGACTCTCCGTCTCGGCGGCCAGATTCTTCGCCGCCGAGGTCCTGGTGGCGCTCGAGTACCTTCACGCGCTCGGGATCGTCTACCGGGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCTCTGATTTCGACTTGTGCTTTAAGTCCGACGTGGTCCCCACTTTTCACACGTGGACGCGGCGGGGACCACAGGGCAGTGGCTGTTTTGGGTTGAGGACGGCGGCGGCGGCGGAGGAGGAGATTGTGGGGGAGTTTGTGGCGGAGCCGACGGCGGCGTTTTCGAAGTCGTGTGTGGGGACTCATGAGTATTTGGCGCCGGAGTTGGTGGCCGGCGGTGGCCACGGCAACGGCGTCGACTGGTGGGCGTTTGGGGTGTTCGTTTACGAGCTCCTGCACGGAACGACGCCGTTTAAAGGAGTCAACAAGGAGGGAACTCTAAGAAACATTGCGTTGTCGGACGGGGTGAAGTTCCGGGCTGTGGCGGCGGCGGAGGAAGAGGAGGGGATGGCGGAGGCGAGGGATCTGATAGAGCGGCTGCTGGTGAAGGATCCGGCTCGGCGGATGGGGTCCGGCAAAGGGGCGACTGAGATCAAACGGCATCCGTTCTTCGACGGGATAAAATGGCCGTTGATCCGGACGTACCGGGCGCCGGAGGTGTATGGGCTGATGAGAAAAGCCAGATCGCACGTGAGCCACGTGACTGGCGTGACCCACCGCATGAGGCGGCGGGGGTGGTGGAACTGGAAGAAGCTGAGTAACCGTTTAATGAAAAATGGAAGTTCCAGTAAATCTCATACCAATACCAATTATTACAGTTATAGCGCCAAAAATAAGAAGGGTTAA

Protein sequence

MDMDDDFSIFPPDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRLSAAAAALNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFHTWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYSYSAKNKKG
Homology
BLAST of Spg004265 vs. NCBI nr
Match: XP_038899976.1 (serine/threonine-protein kinase WAG1-like [Benincasa hispida])

HSP 1 Score: 844.7 bits (2181), Expect = 3.8e-241
Identity = 438/479 (91.44%), Postives = 451/479 (94.15%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTF-ASSSARSSLARTSLTLSFNESRLSAAAAA- 62
           M+ D+SIFPPDSDLDFSF  TSTATDRTF  SSSARSSLAR+SLTLSFNESRLSAAAAA 
Sbjct: 1   MEYDYSIFPPDSDLDFSF--TSTATDRTFTTSSSARSSLARSSLTLSFNESRLSAAAAAA 60

Query: 63  --LNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHA 122
             +N  PHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHA
Sbjct: 61  AIVNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHA 120

Query: 123 SFALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDL 182
           SFALKVVD+EALSNKKLLQVQTEAEILA LDHPFLPTLY RLDVSHYTCLLIDYCPAGDL
Sbjct: 121 SFALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDL 180

Query: 183 HSLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFD 242
           HSLLRKQPGNR SV+AARFFAAEVLVALEYLH+LGIVYRDLKPENVLLREDGHVMLSDFD
Sbjct: 181 HSLLRKQPGNRFSVAAARFFAAEVLVALEYLHSLGIVYRDLKPENVLLREDGHVMLSDFD 240

Query: 243 LCFKSDVVPTFHTWTRRG-PQGSG-CFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHE 302
           LCFKSDVVPTFHTWTR G PQ +G CFG RT A  EEEIVGEFVAEPTAAFSKSCVGTHE
Sbjct: 241 LCFKSDVVPTFHTWTRPGPPQPTGCCFGWRT-AEEEEEIVGEFVAEPTAAFSKSCVGTHE 300

Query: 303 YLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAA 362
           YLAPELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+G+KFR   A
Sbjct: 301 YLAPELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGMKFR--PA 360

Query: 363 AEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGL 422
           AEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGL
Sbjct: 361 AEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGL 420

Query: 423 MRKARSHVSHVTGVTHRMRRRGWWNWKKL-SNRLMKNGSSSKSHTNTNYYSYSAKNKKG 475
           MRKARSHVSHVTGVTHRMRRRGWWNW+KL SNRL+KNGSSSKSHTNTNYYSYS KNK G
Sbjct: 421 MRKARSHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSFKNKTG 474

BLAST of Spg004265 vs. NCBI nr
Match: XP_023553017.1 (serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 831.6 bits (2147), Expect = 3.3e-237
Identity = 414/471 (87.90%), Postives = 439/471 (93.21%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRL---SAAAA 62
           M+DD+S FPPDSDLDFSFTST+  TDRTFASSSAR+SLAR+SLTLSFNESRL   +AAAA
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTT--TDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAA 60

Query: 63  ALNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 122
           A+N  PH HSDPHWSAIKAAT LSSDGHLHLRHLKL+RHLGTGNLGRVFLCHLRDNDHAS
Sbjct: 61  AVNHRPHHHSDPHWSAIKAATALSSDGHLHLRHLKLVRHLGTGNLGRVFLCHLRDNDHAS 120

Query: 123 FALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLH 182
           FALKVVD++ALSNKKLLQVQTE+EILA L+HPFLPTLY RLDVSHYTCLLIDYCPAGDLH
Sbjct: 121 FALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPAGDLH 180

Query: 183 SLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 242
           SLLRKQP  R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL
Sbjct: 181 SLLRKQPNYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 240

Query: 243 CFKSDVVPTFHTWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLA 302
           CFKS+VVPTFHTWT+ GPQ + CFG RTAAA  +EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 CFKSEVVPTFHTWTQPGPQSTSCFGWRTAAAESQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 303 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEE 362
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+ VKFR   AA E
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEAVKFR---AAVE 360

Query: 363 EEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 422
           EEGMAEARDL+ERLLVKDPARR+GSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK
Sbjct: 361 EEGMAEARDLVERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 420

Query: 423 ARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYSYSAK 471
           ARSHVSHVTGV+HRMRRRGWWNW+KLSN L KNG++SKSHTN NYYSYS+K
Sbjct: 421 ARSHVSHVTGVSHRMRRRGWWNWRKLSNGLFKNGNASKSHTNNNYYSYSSK 466

BLAST of Spg004265 vs. NCBI nr
Match: XP_022984745.1 (serine/threonine-protein kinase WAG1-like [Cucurbita maxima])

HSP 1 Score: 823.9 bits (2127), Expect = 6.9e-235
Identity = 411/467 (88.01%), Postives = 434/467 (92.93%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRL---SAAAA 62
           M+DD+S FPPDSDLDFSFTST+  TDRTFASSSAR+SLAR+SLTLSFNESRL   +AAAA
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTT--TDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAA 60

Query: 63  ALNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 122
           A++  PH HSDPHWSAIKAAT LSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS
Sbjct: 61  AVHHRPHHHSDPHWSAIKAATALSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 120

Query: 123 FALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLH 182
           FALKVVD++ALSNKKLLQVQTE+EILA L+HPFLPTLY RLDVSHYTCLLIDYCP+GDLH
Sbjct: 121 FALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPSGDLH 180

Query: 183 SLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 242
           SLLRKQP  R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL
Sbjct: 181 SLLRKQPNYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 240

Query: 243 CFKSDVVPTFHTWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLA 302
           CFKS+VVPTFHTWT+ GPQ + CFG RTAA   +EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 CFKSEVVPTFHTWTQPGPQSTSCFGWRTAAVESQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 303 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEE 362
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+GVKFR   AA E
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFR---AAVE 360

Query: 363 EEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 422
           EEGMAEARDLIERLLVKDPARR+GSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK
Sbjct: 361 EEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 420

Query: 423 ARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYS 467
           ARSHVSHVTG +HRMRRRGWWNW+KLSN L KNGS+SKSHTN NYYS
Sbjct: 421 ARSHVSHVTGASHRMRRRGWWNWRKLSNGLFKNGSASKSHTNNNYYS 462

BLAST of Spg004265 vs. NCBI nr
Match: XP_022931531.1 (serine/threonine-protein kinase WAG1-like [Cucurbita moschata])

HSP 1 Score: 819.7 bits (2116), Expect = 1.3e-233
Identity = 411/471 (87.26%), Postives = 436/471 (92.57%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRL---SAAAA 62
           M+DD+S FPPDSDLDFSFTST+  TDRTFASSSAR+SLAR+SLTLSFNESRL   +AAAA
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTT--TDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAA 60

Query: 63  ALNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 122
           A+N  PH HSDPHWSAIKAAT LSSDG LHLR+LKL+RHLGTGNLGRVFLCHLRDNDHAS
Sbjct: 61  AVNHRPHHHSDPHWSAIKAATALSSDGRLHLRNLKLVRHLGTGNLGRVFLCHLRDNDHAS 120

Query: 123 FALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLH 182
           FALKVVD++ALSNKKLLQVQTE+EILA L+HPFLPTLY RLDVSHYTCLLIDYCPAGDLH
Sbjct: 121 FALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPAGDLH 180

Query: 183 SLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 242
           SLLRKQP  R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL
Sbjct: 181 SLLRKQPSYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 240

Query: 243 CFKSDVVPTFHTWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLA 302
           CFKS+VVPTFHTWT+ GPQ + CFG RTAAA  +EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 CFKSEVVPTFHTWTQPGPQSTSCFGWRTAAAEPQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 303 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEE 362
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+ VKFR   AA E
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEAVKFR---AAVE 360

Query: 363 EEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 422
           EEGMAEARDLIERLLVKDPARR+GSGKGATEIKRH FFDGIKWPLIRTYRAPEVYGLMRK
Sbjct: 361 EEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHTFFDGIKWPLIRTYRAPEVYGLMRK 420

Query: 423 ARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYSYSAK 471
           ARSHVSHVTGV+HRMRRR WWNW+KLSN L KNG++SKSHTN NYYSYS+K
Sbjct: 421 ARSHVSHVTGVSHRMRRRTWWNWRKLSNGLFKNGNASKSHTNNNYYSYSSK 466

BLAST of Spg004265 vs. NCBI nr
Match: XP_008451816.1 (PREDICTED: serine/threonine-protein kinase WAG1 [Cucumis melo] >TYK16379.1 serine/threonine-protein kinase WAG1 [Cucumis melo var. makuwa])

HSP 1 Score: 810.4 bits (2092), Expect = 7.9e-231
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTF-ASSSARSSLARTSLTLSFNESRLSAAAAAL 62
           M+DD+SIFPPDSDLDFSFTST+  TDRTF  SSSAR+SLAR+SLTLSFNESRLSAAAAAL
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTT--TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAL 60

Query: 63  NQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 122
           N  PH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA
Sbjct: 61  NLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 120

Query: 123 LKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSL 182
           LKVVD+EALSNKKL QVQTEAEILA LDHPFLPTLY RLDVSHYTCLLIDYCPAGDLHSL
Sbjct: 121 LKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSL 180

Query: 183 LRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 242
           LRKQPG+R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF
Sbjct: 181 LRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 240

Query: 243 KSDVVPTFHTWTRRGPQGSG-CFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 302
           KSDVVPTFHTWTR GP+ +G C G RT    EEEIVGEFVAEPTAAFSKSCVGTHEYLAP
Sbjct: 241 KSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 300

Query: 303 ELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEEE 362
           ELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+GVKFR  AA EEE
Sbjct: 301 ELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFR--AAGEEE 360

Query: 363 EGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKA 422
           EGM EARDLIERLLVKDPA R+GS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK 
Sbjct: 361 EGMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKG 420

Query: 423 RSHVSHVTGVTHRMRRRGWWNWKK-LSNRLMKNGSSSKSHTNTNYYSYSAKNKKG 475
           RS VSHV GV+ R+RRRGWWNW+K +SN L+KNGS SKSH+NTN+YSYS+K K G
Sbjct: 421 RSQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Spg004265 vs. ExPASy Swiss-Prot
Match: Q9C8M5 (Serine/threonine-protein kinase WAG1 OS=Arabidopsis thaliana OX=3702 GN=WAG1 PE=2 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 9.0e-169
Identity = 314/476 (65.97%), Postives = 360/476 (75.63%), Query Frame = 0

Query: 13  DSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRLSAA--------AAALNQH 72
           D+DLD SF  TSTATDRTF SSSARSSLAR+SLTLSFN+ RLS A        +AA   H
Sbjct: 9   DTDLDLSF--TSTATDRTFTSSSARSSLARSSLTLSFND-RLSTATTPSTTTSSAATTLH 68

Query: 73  PHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRD-NDHASFALK 132
            HR  DPHW++I+AATTLSSDG LHLRH KL+RHLGTGNLGRVFLCHLRD  +   FALK
Sbjct: 69  -HRRYDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRVFLCHLRDCPNPTGFALK 128

Query: 133 VVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLR 192
           V+DR+ L+ KK+  V+TEAEIL+LLDHPFLPTLY R+D SHYTCLLIDYCP GDLHSLLR
Sbjct: 129 VIDRDVLTAKKISHVETEAEILSLLDHPFLPTLYARIDASHYTCLLIDYCPNGDLHSLLR 188

Query: 193 KQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKS 252
           KQP NRL +S  RFFAAEVLVALEYLHALGIVYRDLKPEN+L+REDGH+MLSDFDLCFK+
Sbjct: 189 KQPNNRLPISPVRFFAAEVLVALEYLHALGIVYRDLKPENILIREDGHIMLSDFDLCFKA 248

Query: 253 DVVPTFH--------TWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTH 312
           DVVPTF         +  R+  +G GCF         EEIV EF AEP  AFSKSCVGTH
Sbjct: 249 DVVPTFRSRRFRRTSSSPRKTRRGGGCFSTE-VEYEREEIVAEFAAEPVTAFSKSCVGTH 308

Query: 313 EYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVA 372
           EYLAPELVAG GHG+GVDWWAFG+F+YE+L+GTTPFKG  KE TLRNI  +D V F    
Sbjct: 309 EYLAPELVAGNGHGSGVDWWAFGIFLYEMLYGTTPFKGGTKEQTLRNIVSNDDVAFTL-- 368

Query: 373 AAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYG 432
              EEEGM EA+DLIE+LLVKDP +R+G  +GA +IKRH FF+GIKWPLIR Y+ PE+ G
Sbjct: 369 ---EEEGMVEAKDLIEKLLVKDPRKRLGCARGAQDIKRHEFFEGIKWPLIRNYKPPEIRG 428

Query: 433 LMRKARSHVSHVT-GVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYSYSAK 471
           L++K ++H  HVT  VT R  +  WW    L      + SSSK  +N NYY Y  K
Sbjct: 429 LVKKTKAHAGHVTAAVTPRRNKWLWWALSHLLRSKSLSKSSSKIQSNNNYYHYVGK 474

BLAST of Spg004265 vs. ExPASy Swiss-Prot
Match: Q9LUL2 (Serine/threonine-protein kinase WAG2 OS=Arabidopsis thaliana OX=3702 GN=WAG2 PE=2 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 4.2e-158
Identity = 313/481 (65.07%), Postives = 357/481 (74.22%), Query Frame = 0

Query: 12  PDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRLSAAAAALNQHP------ 71
           PD+DLD SFTST+  TDRTFASSS     ARTSLTLSFN+ RLS ++A            
Sbjct: 9   PDTDLDLSFTSTT--TDRTFASSS-----ARTSLTLSFND-RLSTSSAVTTSSTSSSSVN 68

Query: 72  HRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVV 131
           HR  DPHWSAIK+A  LSSDG++HLRHLKLIRHLGTGNLGRVFLC+LRD+  A FALKV+
Sbjct: 69  HRRHDPHWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVFLCNLRDSS-ARFALKVI 128

Query: 132 DREAL-SNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRK 191
           DR  L + KKL QV+TEAEIL+LLDHPFLPTLY R+D SHYTCLLIDY P GDLHSLLRK
Sbjct: 129 DRNCLTTEKKLSQVETEAEILSLLDHPFLPTLYARIDESHYTCLLIDYAPNGDLHSLLRK 188

Query: 192 QPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSD 251
           QPGNRL +   RFFAAEVLVALEYLHA+GIVYRDLKPENVLLREDGHVMLSDFDLCFKSD
Sbjct: 189 QPGNRLPIQPVRFFAAEVLVALEYLHAMGIVYRDLKPENVLLREDGHVMLSDFDLCFKSD 248

Query: 252 VVPTFHTWTRRGPQG--------SGCFGLRTAAAAE-EEIVGEFVAEPTAAFSKSCVGTH 311
           VVPTF +   R            SGCF +      E EEIV EF AEP  AFS+SCVGTH
Sbjct: 249 VVPTFKSRRYRRSSSSPSLRRRRSGCFSVAAEKKYEREEIVSEFAAEPVTAFSRSCVGTH 308

Query: 312 EYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVA 371
           EYLAPELV+G GHG+GVDWWAFG+F+YELL+GTTPFKG +KE TLRNI     V     A
Sbjct: 309 EYLAPELVSGNGHGSGVDWWAFGIFLYELLYGTTPFKGESKEQTLRNI-----VSTTKTA 368

Query: 372 AAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAP-EVY 431
           +   +  + EARDLIE+LLVKDP +R+G  +GA +IKRHPFFDGIKWPLIR Y+ P EV 
Sbjct: 369 SFHMDGDLDEARDLIEKLLVKDPRKRLGCARGAQDIKRHPFFDGIKWPLIRHYKPPEEVR 428

Query: 432 GLM--RKARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSS--SKSHTNTNYYSYSAK 472
           GL+  +  R H SHV  V+   RRR  + W+ LS  L    SS  SK+ +N+NYY Y  K
Sbjct: 429 GLVIKKSTRPHASHVIAVS--PRRRKSFLWRALSYLLRGKSSSGGSKNQSNSNYYHYVGK 473

BLAST of Spg004265 vs. ExPASy Swiss-Prot
Match: Q94E49 (Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-89
Identity = 198/465 (42.58%), Postives = 265/465 (56.99%), Query Frame = 0

Query: 13  DSDLDFSFTS-TSTATDRTFASSSARSSLARTSLTLSFNESRLSAAAAALNQHPHRHSDP 72
           +SD D S +S T+  + R++ S    SS      ++S         A++    PH+ +  
Sbjct: 7   ESDYDSSRSSLTAPDSRRSWISDIGSSS------SVSARSFGGDTPASSCRYKPHKANQA 66

Query: 73  HWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS----------FA 132
            W AI+     +  G + L H +L+R LG+G+LG V+LC LR+   +S          +A
Sbjct: 67  EWEAIRRLR--AGAGRVGLEHFRLVRRLGSGDLGNVYLCRLREPWSSSSMTTTAGGCLYA 126

Query: 133 LKVVDREALS-NKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHS 192
           +KVVD++AL+  KKL + + E +IL  LDHPFLPTLY   + SHY CL++++CP GDLH 
Sbjct: 127 MKVVDKDALAFRKKLRRAEVERDILRTLDHPFLPTLYADFEASHYACLVMEFCPGGDLHV 186

Query: 193 LLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 252
             ++QPG R +VS+ RF+ AE ++ALEYLH +G+VYRDLKPENVL+R DGH+MLSDFDL 
Sbjct: 187 ARQRQPGRRFTVSSTRFYVAETVLALEYLHMMGVVYRDLKPENVLVRGDGHIMLSDFDLS 246

Query: 253 FKSDVVPTFHTWTRRGPQG---------------------------------SGCFGLRT 312
            K DVVP      R    G                                   C G   
Sbjct: 247 LKCDVVPKLLRPARSAAAGGKPPLPPPSSCVPPTIQPVLSCIFRGVHKCHHAKECAGGGA 306

Query: 313 AA----------------AAEEEIVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNG 372
           AA                 AE E+V   V EP AA SKS VGTHEYLAPE+++G GHG+ 
Sbjct: 307 AAGNNGDGDGNDEEAETETAEPEVV---VVEPVAARSKSFVGTHEYLAPEVISGQGHGSA 366

Query: 373 VDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKF---RAVAAAEEEEGMAEARD 414
           VDWW  GVF+YE+L+G TPFKG + E TL NI +   V F      AAA E E M  A+D
Sbjct: 367 VDWWTLGVFMYEMLYGRTPFKGESNEKTLINI-IKQPVTFPRLAGAAAAGEWEEMKTAQD 426

BLAST of Spg004265 vs. ExPASy Swiss-Prot
Match: Q9SUA3 (Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1 PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.4e-89
Identity = 184/418 (44.02%), Postives = 248/418 (59.33%), Query Frame = 0

Query: 32  ASSSARSSLARTSLTLSFNESRLSAAAAALNQHPHRHSDPHWSAIKAATTLSSDGHLHLR 91
           +  S +S+  R S +   ++    ++ +  N  PH+ +D  W AI+A  T    G L L 
Sbjct: 65  SDQSGKSNTCRPSTSSDISDESTCSSFSG-NNKPHKANDVRWEAIQAVRT--KHGVLGLN 124

Query: 92  HLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDREAL-SNKKLLQVQTEAEILALLDH 151
           H +L++ LG G++G V L  L       FA+KV+D+ AL S KKLL+ QTE EIL  LDH
Sbjct: 125 HFRLLKRLGCGDIGTVHLAELH-GTRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDH 184

Query: 152 PFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFAAEVLVALEYLH 211
           PFLPTLY+  +   ++CL++++CP GDLH+L ++QPG R S  AA+F+ AEVL+A+EYLH
Sbjct: 185 PFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLH 244

Query: 212 ALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFHTWT---RRGPQGSG------ 271
            LGI+YRDLKPENVL+R+DGHVMLSDFDL  +  V PT    T     G + SG      
Sbjct: 245 MLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKNSGYCAQPA 304

Query: 272 -------------CFG-------------LRTAAAAEEEIVGEFVAEPTAAFSKSCVGTH 331
                        CF              ++     +   + E VAEPT+A S S VGTH
Sbjct: 305 CIQQPSCISAPTTCFSPRYFSSKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTH 364

Query: 332 EYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVA 391
           EYLAPE++ G GHG+ VDWW FG+F+YELL G TPFKG     TL N+ +   ++F    
Sbjct: 365 EYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNV-VGQPLRF---- 424

Query: 392 AAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEV 414
             E       ARDLI  LLVK+P  R+   +GATE+K+HPFF+G+ W L+R    PE+
Sbjct: 425 -PESPVVSFAARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEI 472

BLAST of Spg004265 vs. ExPASy Swiss-Prot
Match: Q9FG74 (Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.0e-88
Identity = 191/444 (43.02%), Postives = 262/444 (59.01%), Query Frame = 0

Query: 14  SDLDFSFTSTSTATDRTFAS---SSARSSLARTSLTLSF-NESRLSAAAAALNQHPHRHS 73
           S L F+  S+   T  +  +    S +SS  R S +    +ES  S+ ++++N+ PH+ +
Sbjct: 29  SSLSFADPSSDGKTGNSKINEQGESGKSSTCRPSTSSDISDESTCSSFSSSINK-PHKAN 88

Query: 74  DPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDREA 133
           D  W AI+A  T    G L L H +L++ LG G++G V L  L +     FA+KV+D+ A
Sbjct: 89  DVRWEAIQAVRT--KHGGLGLNHFRLLKRLGCGDIGTVHLAEL-NGTRCYFAMKVMDKTA 148

Query: 134 L-SNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRKQPGN 193
           L S KKLL+ QTE EIL  LDHPFLPTLY+  +   ++CL++++CP GDLH+L ++QPG 
Sbjct: 149 LASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQPGK 208

Query: 194 RLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPT 253
           R +  AA+F+ AEVL+A+EYLH LGI+YRDLKPENVL+R+DGHVMLSDFDL  +  V  +
Sbjct: 209 RFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVSLS 268

Query: 254 FHTWTRRGPQG-------------------------SGCFGLRTAAAAEEE--------- 313
                  G +G                         + CFG R  ++  ++         
Sbjct: 269 IVRSANVGSEGLSKNSVSCSQQPACIQQPSCISMAPTSCFGPRFFSSKSKKDKKPKTENG 328

Query: 314 -----IVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTT 373
                 + E VAEPT A S S VGTHEYLAPE++ G GHG+ VDWW FG+F+YELL G T
Sbjct: 329 NHQVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKT 388

Query: 374 PFKGVNKEGTLRNIALSDGVKFRAVAAAEEEEGMAEARDLIERLLVKDPARRMGSGKGAT 414
           PFKG     TL N+ +   ++F      E       ARDLI  LLVK+P  R+   +GAT
Sbjct: 389 PFKGSGNRATLFNV-VGQPLRF-----PESPVVSFAARDLIRSLLVKEPQHRLAYKRGAT 448

BLAST of Spg004265 vs. ExPASy TrEMBL
Match: A0A6J1J9F8 (serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC111482931 PE=4 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.4e-235
Identity = 411/467 (88.01%), Postives = 434/467 (92.93%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRL---SAAAA 62
           M+DD+S FPPDSDLDFSFTST+  TDRTFASSSAR+SLAR+SLTLSFNESRL   +AAAA
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTT--TDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAA 60

Query: 63  ALNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 122
           A++  PH HSDPHWSAIKAAT LSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS
Sbjct: 61  AVHHRPHHHSDPHWSAIKAATALSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 120

Query: 123 FALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLH 182
           FALKVVD++ALSNKKLLQVQTE+EILA L+HPFLPTLY RLDVSHYTCLLIDYCP+GDLH
Sbjct: 121 FALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPSGDLH 180

Query: 183 SLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 242
           SLLRKQP  R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL
Sbjct: 181 SLLRKQPNYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 240

Query: 243 CFKSDVVPTFHTWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLA 302
           CFKS+VVPTFHTWT+ GPQ + CFG RTAA   +EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 CFKSEVVPTFHTWTQPGPQSTSCFGWRTAAVESQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 303 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEE 362
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+GVKFR   AA E
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFR---AAVE 360

Query: 363 EEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 422
           EEGMAEARDLIERLLVKDPARR+GSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK
Sbjct: 361 EEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 420

Query: 423 ARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYS 467
           ARSHVSHVTG +HRMRRRGWWNW+KLSN L KNGS+SKSHTN NYYS
Sbjct: 421 ARSHVSHVTGASHRMRRRGWWNWRKLSNGLFKNGSASKSHTNNNYYS 462

BLAST of Spg004265 vs. ExPASy TrEMBL
Match: A0A6J1ETX0 (serine/threonine-protein kinase WAG1-like OS=Cucurbita moschata OX=3662 GN=LOC111437682 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.3e-234
Identity = 411/471 (87.26%), Postives = 436/471 (92.57%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRL---SAAAA 62
           M+DD+S FPPDSDLDFSFTST+  TDRTFASSSAR+SLAR+SLTLSFNESRL   +AAAA
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTT--TDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAA 60

Query: 63  ALNQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS 122
           A+N  PH HSDPHWSAIKAAT LSSDG LHLR+LKL+RHLGTGNLGRVFLCHLRDNDHAS
Sbjct: 61  AVNHRPHHHSDPHWSAIKAATALSSDGRLHLRNLKLVRHLGTGNLGRVFLCHLRDNDHAS 120

Query: 123 FALKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLH 182
           FALKVVD++ALSNKKLLQVQTE+EILA L+HPFLPTLY RLDVSHYTCLLIDYCPAGDLH
Sbjct: 121 FALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPAGDLH 180

Query: 183 SLLRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 242
           SLLRKQP  R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL
Sbjct: 181 SLLRKQPSYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDL 240

Query: 243 CFKSDVVPTFHTWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLA 302
           CFKS+VVPTFHTWT+ GPQ + CFG RTAAA  +EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 CFKSEVVPTFHTWTQPGPQSTSCFGWRTAAAEPQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 303 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEE 362
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+ VKFR   AA E
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEAVKFR---AAVE 360

Query: 363 EEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRK 422
           EEGMAEARDLIERLLVKDPARR+GSGKGATEIKRH FFDGIKWPLIRTYRAPEVYGLMRK
Sbjct: 361 EEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHTFFDGIKWPLIRTYRAPEVYGLMRK 420

Query: 423 ARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYSYSAK 471
           ARSHVSHVTGV+HRMRRR WWNW+KLSN L KNG++SKSHTN NYYSYS+K
Sbjct: 421 ARSHVSHVTGVSHRMRRRTWWNWRKLSNGLFKNGNASKSHTNNNYYSYSSK 466

BLAST of Spg004265 vs. ExPASy TrEMBL
Match: A0A5D3D1P2 (Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001730 PE=4 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.8e-231
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTF-ASSSARSSLARTSLTLSFNESRLSAAAAAL 62
           M+DD+SIFPPDSDLDFSFTST+  TDRTF  SSSAR+SLAR+SLTLSFNESRLSAAAAAL
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTT--TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAL 60

Query: 63  NQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 122
           N  PH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA
Sbjct: 61  NLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 120

Query: 123 LKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSL 182
           LKVVD+EALSNKKL QVQTEAEILA LDHPFLPTLY RLDVSHYTCLLIDYCPAGDLHSL
Sbjct: 121 LKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSL 180

Query: 183 LRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 242
           LRKQPG+R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF
Sbjct: 181 LRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 240

Query: 243 KSDVVPTFHTWTRRGPQGSG-CFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 302
           KSDVVPTFHTWTR GP+ +G C G RT    EEEIVGEFVAEPTAAFSKSCVGTHEYLAP
Sbjct: 241 KSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 300

Query: 303 ELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEEE 362
           ELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+GVKFR  AA EEE
Sbjct: 301 ELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFR--AAGEEE 360

Query: 363 EGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKA 422
           EGM EARDLIERLLVKDPA R+GS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK 
Sbjct: 361 EGMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKG 420

Query: 423 RSHVSHVTGVTHRMRRRGWWNWKK-LSNRLMKNGSSSKSHTNTNYYSYSAKNKKG 475
           RS VSHV GV+ R+RRRGWWNW+K +SN L+KNGS SKSH+NTN+YSYS+K K G
Sbjct: 421 RSQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Spg004265 vs. ExPASy TrEMBL
Match: A0A1S3BRT1 (serine/threonine-protein kinase WAG1 OS=Cucumis melo OX=3656 GN=LOC103492987 PE=4 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.8e-231
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTF-ASSSARSSLARTSLTLSFNESRLSAAAAAL 62
           M+DD+SIFPPDSDLDFSFTST+  TDRTF  SSSAR+SLAR+SLTLSFNESRLSAAAAAL
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTT--TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAL 60

Query: 63  NQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 122
           N  PH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA
Sbjct: 61  NLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 120

Query: 123 LKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSL 182
           LKVVD+EALSNKKL QVQTEAEILA LDHPFLPTLY RLDVSHYTCLLIDYCPAGDLHSL
Sbjct: 121 LKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSL 180

Query: 183 LRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 242
           LRKQPG+R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF
Sbjct: 181 LRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 240

Query: 243 KSDVVPTFHTWTRRGPQGSG-CFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 302
           KSDVVPTFHTWTR GP+ +G C G RT    EEEIVGEFVAEPTAAFSKSCVGTHEYLAP
Sbjct: 241 KSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 300

Query: 303 ELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEEE 362
           ELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA S+GVKFR  AA EEE
Sbjct: 301 ELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFR--AAGEEE 360

Query: 363 EGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKA 422
           EGM EARDLIERLLVKDPA R+GS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK 
Sbjct: 361 EGMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKG 420

Query: 423 RSHVSHVTGVTHRMRRRGWWNWKK-LSNRLMKNGSSSKSHTNTNYYSYSAKNKKG 475
           RS VSHV GV+ R+RRRGWWNW+K +SN L+KNGS SKSH+NTN+YSYS+K K G
Sbjct: 421 RSQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Spg004265 vs. ExPASy TrEMBL
Match: A0A5A7V4J3 (Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold468G00580 PE=4 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 9.4e-230
Identity = 410/475 (86.32%), Postives = 435/475 (91.58%), Query Frame = 0

Query: 3   MDDDFSIFPPDSDLDFSFTSTSTATDRTF-ASSSARSSLARTSLTLSFNESRLSAAAAAL 62
           M+DD+SIFPPDSDLDFSFTST+  TDRTF  SSSAR+SLAR+SLTLSFNESRLSAAAAAL
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTT--TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAL 60

Query: 63  NQHPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 122
           N  PH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA
Sbjct: 61  NLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFA 120

Query: 123 LKVVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSL 182
           LKVVD+EALSNKKL QVQTEAEILA LDHPFLPTLY RLDVSHYTCLLIDYCPAGDLHSL
Sbjct: 121 LKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSL 180

Query: 183 LRKQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 242
           LRKQPG+R SV+AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF
Sbjct: 181 LRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCF 240

Query: 243 KSDVVPTFHTWTRRGPQGSG-CFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 302
           KSDVVPTFHTWTR GP+ +G C G RT    EEEIVGEFVAEPTAAFSKSCVGTHEYLAP
Sbjct: 241 KSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLAP 300

Query: 303 ELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVAAAEEE 362
           EL++GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKE TLRNIA S+GVKFR  AA EEE
Sbjct: 301 ELLSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEVTLRNIASSEGVKFR--AAGEEE 360

Query: 363 EGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKA 422
           EGM EARDLIERLLVKDPA R+GS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK 
Sbjct: 361 EGMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKG 420

Query: 423 RSHVSHVTGVTHRMRRRGWWNWKK-LSNRLMKNGSSSKSHTNTNYYSYSAKNKKG 475
           RS VSHV GV+ R+RRRGWWNW+K +SN L+KNGS SKSH+NTN+YSYS+K K G
Sbjct: 421 RSQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Spg004265 vs. TAIR 10
Match: AT1G53700.1 (WAG 1 )

HSP 1 Score: 594.7 bits (1532), Expect = 6.4e-170
Identity = 314/476 (65.97%), Postives = 360/476 (75.63%), Query Frame = 0

Query: 13  DSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRLSAA--------AAALNQH 72
           D+DLD SF  TSTATDRTF SSSARSSLAR+SLTLSFN+ RLS A        +AA   H
Sbjct: 9   DTDLDLSF--TSTATDRTFTSSSARSSLARSSLTLSFND-RLSTATTPSTTTSSAATTLH 68

Query: 73  PHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRD-NDHASFALK 132
            HR  DPHW++I+AATTLSSDG LHLRH KL+RHLGTGNLGRVFLCHLRD  +   FALK
Sbjct: 69  -HRRYDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRVFLCHLRDCPNPTGFALK 128

Query: 133 VVDREALSNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLR 192
           V+DR+ L+ KK+  V+TEAEIL+LLDHPFLPTLY R+D SHYTCLLIDYCP GDLHSLLR
Sbjct: 129 VIDRDVLTAKKISHVETEAEILSLLDHPFLPTLYARIDASHYTCLLIDYCPNGDLHSLLR 188

Query: 193 KQPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKS 252
           KQP NRL +S  RFFAAEVLVALEYLHALGIVYRDLKPEN+L+REDGH+MLSDFDLCFK+
Sbjct: 189 KQPNNRLPISPVRFFAAEVLVALEYLHALGIVYRDLKPENILIREDGHIMLSDFDLCFKA 248

Query: 253 DVVPTFH--------TWTRRGPQGSGCFGLRTAAAAEEEIVGEFVAEPTAAFSKSCVGTH 312
           DVVPTF         +  R+  +G GCF         EEIV EF AEP  AFSKSCVGTH
Sbjct: 249 DVVPTFRSRRFRRTSSSPRKTRRGGGCFSTE-VEYEREEIVAEFAAEPVTAFSKSCVGTH 308

Query: 313 EYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVA 372
           EYLAPELVAG GHG+GVDWWAFG+F+YE+L+GTTPFKG  KE TLRNI  +D V F    
Sbjct: 309 EYLAPELVAGNGHGSGVDWWAFGIFLYEMLYGTTPFKGGTKEQTLRNIVSNDDVAFTL-- 368

Query: 373 AAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYG 432
              EEEGM EA+DLIE+LLVKDP +R+G  +GA +IKRH FF+GIKWPLIR Y+ PE+ G
Sbjct: 369 ---EEEGMVEAKDLIEKLLVKDPRKRLGCARGAQDIKRHEFFEGIKWPLIRNYKPPEIRG 428

Query: 433 LMRKARSHVSHVT-GVTHRMRRRGWWNWKKLSNRLMKNGSSSKSHTNTNYYSYSAK 471
           L++K ++H  HVT  VT R  +  WW    L      + SSSK  +N NYY Y  K
Sbjct: 429 LVKKTKAHAGHVTAAVTPRRNKWLWWALSHLLRSKSLSKSSSKIQSNNNYYHYVGK 474

BLAST of Spg004265 vs. TAIR 10
Match: AT3G14370.1 (Protein kinase superfamily protein )

HSP 1 Score: 559.3 bits (1440), Expect = 3.0e-159
Identity = 313/481 (65.07%), Postives = 357/481 (74.22%), Query Frame = 0

Query: 12  PDSDLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRLSAAAAALNQHP------ 71
           PD+DLD SFTST+  TDRTFASSS     ARTSLTLSFN+ RLS ++A            
Sbjct: 9   PDTDLDLSFTSTT--TDRTFASSS-----ARTSLTLSFND-RLSTSSAVTTSSTSSSSVN 68

Query: 72  HRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVV 131
           HR  DPHWSAIK+A  LSSDG++HLRHLKLIRHLGTGNLGRVFLC+LRD+  A FALKV+
Sbjct: 69  HRRHDPHWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVFLCNLRDSS-ARFALKVI 128

Query: 132 DREAL-SNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRK 191
           DR  L + KKL QV+TEAEIL+LLDHPFLPTLY R+D SHYTCLLIDY P GDLHSLLRK
Sbjct: 129 DRNCLTTEKKLSQVETEAEILSLLDHPFLPTLYARIDESHYTCLLIDYAPNGDLHSLLRK 188

Query: 192 QPGNRLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSD 251
           QPGNRL +   RFFAAEVLVALEYLHA+GIVYRDLKPENVLLREDGHVMLSDFDLCFKSD
Sbjct: 189 QPGNRLPIQPVRFFAAEVLVALEYLHAMGIVYRDLKPENVLLREDGHVMLSDFDLCFKSD 248

Query: 252 VVPTFHTWTRRGPQG--------SGCFGLRTAAAAE-EEIVGEFVAEPTAAFSKSCVGTH 311
           VVPTF +   R            SGCF +      E EEIV EF AEP  AFS+SCVGTH
Sbjct: 249 VVPTFKSRRYRRSSSSPSLRRRRSGCFSVAAEKKYEREEIVSEFAAEPVTAFSRSCVGTH 308

Query: 312 EYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVA 371
           EYLAPELV+G GHG+GVDWWAFG+F+YELL+GTTPFKG +KE TLRNI     V     A
Sbjct: 309 EYLAPELVSGNGHGSGVDWWAFGIFLYELLYGTTPFKGESKEQTLRNI-----VSTTKTA 368

Query: 372 AAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAP-EVY 431
           +   +  + EARDLIE+LLVKDP +R+G  +GA +IKRHPFFDGIKWPLIR Y+ P EV 
Sbjct: 369 SFHMDGDLDEARDLIEKLLVKDPRKRLGCARGAQDIKRHPFFDGIKWPLIRHYKPPEEVR 428

Query: 432 GLM--RKARSHVSHVTGVTHRMRRRGWWNWKKLSNRLMKNGSS--SKSHTNTNYYSYSAK 472
           GL+  +  R H SHV  V+   RRR  + W+ LS  L    SS  SK+ +N+NYY Y  K
Sbjct: 429 GLVIKKSTRPHASHVIAVS--PRRRKSFLWRALSYLLRGKSSSGGSKNQSNSNYYHYVGK 473

BLAST of Spg004265 vs. TAIR 10
Match: AT2G44830.1 (Protein kinase superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 7.0e-92
Identity = 198/437 (45.31%), Postives = 258/437 (59.04%), Query Frame = 0

Query: 15  DLDFSFTSTSTATDRTFASSSARSSLARTSLTLSFNESRLSAAAAALNQHPHRHSDPHWS 74
           D ++   S S   D +F S+   +S A  S  LS  ES  S    +LN+ PH+ +DP W+
Sbjct: 290 DKNYFDRSISMVLD-SFESTKTSASRASDSSGLS-EESSWSNFTGSLNK-PHKGNDPWWN 349

Query: 75  AIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDREALSN-K 134
           AI A  T   DG L + H KL++ LG G++G V+L  L       FA+KV+D+ +L + K
Sbjct: 350 AILAIRT--RDGILGMSHFKLLKRLGCGDIGSVYLAEL-SGTRCHFAVKVMDKASLEDRK 409

Query: 135 KLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVS 194
           KL + QTE +IL LLDHPFLPTLYT  +   ++CL+++YCP GDLH+L ++QPG   S  
Sbjct: 410 KLNRAQTERDILQLLDHPFLPTLYTHFETDRFSCLVMEYCPGGDLHTLRQRQPGKHFSEY 469

Query: 195 AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFHTWT 254
           AARF+AAEVL+ALEYLH LG+VYRDLKPENVL+R+DGH+MLSDFDL  +  V PT     
Sbjct: 470 AARFYAAEVLLALEYLHMLGVVYRDLKPENVLVRDDGHIMLSDFDLSLRCAVSPTLIKTF 529

Query: 255 RRGPQGSGCFGLRTAA-------------------------------------AAEEEIV 314
              P   G F ++ A                                       +    +
Sbjct: 530 DSDPSRRGAFCVQPACMEPTSACIIQPSCFLPRSIFPNKNKKNKSRKTQADFFKSHSGSL 589

Query: 315 GEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNK 374
            E VAEP    S S VGTHEYLAPE++ G GHG+ VDWW FG+FV+ELL+G TPFKG   
Sbjct: 590 PELVAEPNTR-SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFVHELLYGKTPFKGSGN 649

Query: 375 EGTLRNIALSDGVKFRAVAAAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPF 414
             TL N+ + + +KF      E        RDLI+ LLVKDP  R+G+ +GATEIK+HPF
Sbjct: 650 RATLFNV-VGEQLKF-----PESPATSYAGRDLIQALLVKDPKNRLGTKRGATEIKQHPF 709

BLAST of Spg004265 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 330.9 bits (847), Expect = 1.7e-90
Identity = 184/418 (44.02%), Postives = 248/418 (59.33%), Query Frame = 0

Query: 32  ASSSARSSLARTSLTLSFNESRLSAAAAALNQHPHRHSDPHWSAIKAATTLSSDGHLHLR 91
           +  S +S+  R S +   ++    ++ +  N  PH+ +D  W AI+A  T    G L L 
Sbjct: 65  SDQSGKSNTCRPSTSSDISDESTCSSFSG-NNKPHKANDVRWEAIQAVRT--KHGVLGLN 124

Query: 92  HLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDREAL-SNKKLLQVQTEAEILALLDH 151
           H +L++ LG G++G V L  L       FA+KV+D+ AL S KKLL+ QTE EIL  LDH
Sbjct: 125 HFRLLKRLGCGDIGTVHLAELH-GTRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDH 184

Query: 152 PFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFAAEVLVALEYLH 211
           PFLPTLY+  +   ++CL++++CP GDLH+L ++QPG R S  AA+F+ AEVL+A+EYLH
Sbjct: 185 PFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLH 244

Query: 212 ALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFHTWT---RRGPQGSG------ 271
            LGI+YRDLKPENVL+R+DGHVMLSDFDL  +  V PT    T     G + SG      
Sbjct: 245 MLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKNSGYCAQPA 304

Query: 272 -------------CFG-------------LRTAAAAEEEIVGEFVAEPTAAFSKSCVGTH 331
                        CF              ++     +   + E VAEPT+A S S VGTH
Sbjct: 305 CIQQPSCISAPTTCFSPRYFSSKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTH 364

Query: 332 EYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIALSDGVKFRAVA 391
           EYLAPE++ G GHG+ VDWW FG+F+YELL G TPFKG     TL N+ +   ++F    
Sbjct: 365 EYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNV-VGQPLRF---- 424

Query: 392 AAEEEEGMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEV 414
             E       ARDLI  LLVK+P  R+   +GATE+K+HPFF+G+ W L+R    PE+
Sbjct: 425 -PESPVVSFAARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEI 472

BLAST of Spg004265 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 327.8 bits (839), Expect = 1.5e-89
Identity = 191/444 (43.02%), Postives = 262/444 (59.01%), Query Frame = 0

Query: 14  SDLDFSFTSTSTATDRTFAS---SSARSSLARTSLTLSF-NESRLSAAAAALNQHPHRHS 73
           S L F+  S+   T  +  +    S +SS  R S +    +ES  S+ ++++N+ PH+ +
Sbjct: 29  SSLSFADPSSDGKTGNSKINEQGESGKSSTCRPSTSSDISDESTCSSFSSSINK-PHKAN 88

Query: 74  DPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDREA 133
           D  W AI+A  T    G L L H +L++ LG G++G V L  L +     FA+KV+D+ A
Sbjct: 89  DVRWEAIQAVRT--KHGGLGLNHFRLLKRLGCGDIGTVHLAEL-NGTRCYFAMKVMDKTA 148

Query: 134 L-SNKKLLQVQTEAEILALLDHPFLPTLYTRLDVSHYTCLLIDYCPAGDLHSLLRKQPGN 193
           L S KKLL+ QTE EIL  LDHPFLPTLY+  +   ++CL++++CP GDLH+L ++QPG 
Sbjct: 149 LASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQPGK 208

Query: 194 RLSVSAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPT 253
           R +  AA+F+ AEVL+A+EYLH LGI+YRDLKPENVL+R+DGHVMLSDFDL  +  V  +
Sbjct: 209 RFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVSLS 268

Query: 254 FHTWTRRGPQG-------------------------SGCFGLRTAAAAEEE--------- 313
                  G +G                         + CFG R  ++  ++         
Sbjct: 269 IVRSANVGSEGLSKNSVSCSQQPACIQQPSCISMAPTSCFGPRFFSSKSKKDKKPKTENG 328

Query: 314 -----IVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTT 373
                 + E VAEPT A S S VGTHEYLAPE++ G GHG+ VDWW FG+F+YELL G T
Sbjct: 329 NHQVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKT 388

Query: 374 PFKGVNKEGTLRNIALSDGVKFRAVAAAEEEEGMAEARDLIERLLVKDPARRMGSGKGAT 414
           PFKG     TL N+ +   ++F      E       ARDLI  LLVK+P  R+   +GAT
Sbjct: 389 PFKGSGNRATLFNV-VGQPLRF-----PESPVVSFAARDLIRSLLVKEPQHRLAYKRGAT 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899976.13.8e-24191.44serine/threonine-protein kinase WAG1-like [Benincasa hispida][more]
XP_023553017.13.3e-23787.90serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo][more]
XP_022984745.16.9e-23588.01serine/threonine-protein kinase WAG1-like [Cucurbita maxima][more]
XP_022931531.11.3e-23387.26serine/threonine-protein kinase WAG1-like [Cucurbita moschata][more]
XP_008451816.17.9e-23186.74PREDICTED: serine/threonine-protein kinase WAG1 [Cucumis melo] >TYK16379.1 serin... [more]
Match NameE-valueIdentityDescription
Q9C8M59.0e-16965.97Serine/threonine-protein kinase WAG1 OS=Arabidopsis thaliana OX=3702 GN=WAG1 PE=... [more]
Q9LUL24.2e-15865.07Serine/threonine-protein kinase WAG2 OS=Arabidopsis thaliana OX=3702 GN=WAG2 PE=... [more]
Q94E491.8e-8942.58Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV... [more]
Q9SUA32.4e-8944.02Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1... [more]
Q9FG742.0e-8843.02Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1J9F83.4e-23588.01serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1ETX06.3e-23487.26serine/threonine-protein kinase WAG1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5D3D1P23.8e-23186.74Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BRT13.8e-23186.74serine/threonine-protein kinase WAG1 OS=Cucumis melo OX=3656 GN=LOC103492987 PE=... [more]
A0A5A7V4J39.4e-23086.32Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT1G53700.16.4e-17065.97WAG 1 [more]
AT3G14370.13.0e-15965.07Protein kinase superfamily protein [more]
AT2G44830.17.0e-9245.31Protein kinase superfamily protein [more]
AT4G26610.11.7e-9044.02D6 protein kinase like 1 [more]
AT5G55910.11.5e-8943.02D6 protein kinase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 356..376
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 288..410
e-value: 2.0E-31
score: 111.0
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 92..176
e-value: 1.5E-41
score: 144.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 177..243
e-value: 1.5E-41
score: 144.3
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 37..238
e-value: 6.7E-25
score: 85.5
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 98..238
e-value: 1.6E-6
score: 25.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 450..474
NoneNo IPR availablePANTHERPTHR45637FLIPPASE KINASE 1-RELATEDcoord: 12..430
NoneNo IPR availablePANTHERPTHR45637:SF2SERINE/THREONINE-PROTEIN KINASE WAG1coord: 12..430
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 93..397
e-value: 1.5E-75
score: 267.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 95..397
e-value: 1.2E-52
score: 178.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 93..397
score: 42.68964
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 214..226
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 91..465

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004265.1Spg004265.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity