Spg004228 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg004228
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectinesterase
Location	scaffold6: 7166923 .. 7173215 (+)
RNA-Seq Expression	Spg004228
Synteny	Spg004228

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATTATCAATCATAATCTCCTCACAAACATTGCTCTCTCTTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCCCGCCACTCGCTTTCTCTCCGACCTCGAGTTTTCCTTCCTCCGCCACCTCGTCGACTGCCATCATCATCACCACCACCACCGTCACCACTACCATCACCATCATCACCATCACAAAAGTCCGACGGACGAAGAAGACCAAGCAACCATCAGTTCCATTTGCGACACGTTCCCAACCAACCTTCTCCCACCAGAAACCACCAACACTTCTGTTTTCTGCGTCGATCGGAACGGTTGTTGCAACTTCACGACGGTTCAGGCTGCAGTCGACTCCGTACCGAACCTGAGCCCGAAACGGAGCGTGATATGGATCAACAGTGGAATCTACTAGTAAATATAATACTAGGGTTTCGTTTTTTTTTTTTTCATAGAAACTTCATTTTATTTTTGTTTTGGCTTGAATGGGAGGTTTTTTTTTGGAGTTGTAGTGAGAAGGTGATGGTTCCAAGATCAAAACCGAACGTGACATTTCAAGGACAAGGCTACACGACCACGGCCATTGTTTGGAACGACACTGCCAACTCTTCGCATGGGACTTTTTACAGTGGCTCTGTTCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGGTAAGTTTATAACTATTAATTAAAATTAGTTTATGAAACACATAAATCATTGAAGATTATTTCTAGACATATTTAAAATTCTCTTCTACGCTAATTTATAATTTTTAATTGACTAGATCTTTTTTAAAAAAAAAAAAAAATGACTTTTTGAAACAAAACATAGAACTATTATCAAATAGAGACTTACAAAATAAAACATAAGTTAAAAAATTTAAATATAATACATCTGTTGTTCATTTTCAAGTGTAAATACAAATAATTTGCTTAAGAAAAAAAAATTGGCACATGAAATATTTTTTGTTGGTTGTAGCTAAAGTGATTGAGATGTATTAGTTATGGTTATATAAAGCTTGCTGTATTATCGTCAAAGCGAGCATAGCTTAGCGGTAATTGATATGTATCTTCGACTAGGACGTTAGAGGTTTGAATCCCCCACTCCAAATGTTCTTAAACTTTAGGAGTATAAGAACTATTGAAACTAAGGGTCCGTTTGGAATACATTTTAATTTAAATGTTTTTCAATAATGAATTTTTAAAAAAACATTTTGCATAAAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTACATTTCCCAAGTGTTTTTATACATTAGTTATTGTATACTTAAAAACTCTTTTTGAAAAAGTTGGTTTCAAGTGTTTCCCTCAAAATGATTTATTTTTCCATCTCATTTTTTTGATAAGTCATTTTTAGTGGTTGCCAAACACTAGAAATTTTTTCAAAATGATTTTTTTTTTCAAAATGATTTTTTTTTTCAAAATTAAACACTTGGAAAGTTAAACCAAACACACCCTAAATTAAAGTAGTTGACAATGACTAAACATGCAATTAAACCTCTCTTTTTTTTTTTTTTTAGTTCTATTAAACTTCAATTTAGAGTTAGTAGATATAACTTGTTTGATTATAATTTTGCTTTTATGAATTGTATTTCTTTATTGATAAGTTTTTATTGGTAATCTATTTTTTTTTTAAAAGAAAATTATATAAAATTTAGAAATTTTAAAGTATATAATATAGGGCCCATTTCTATAACATCTTTTTTTTTTCGTAATTTATTTTAAAAATCTTTCTTATATAGCATTGTTCTATTTATTTTATAATTAATGTCTAGAAATTTTTTTAAAATTTTAATTAAAATTTAATTCTGATATAGTCTGATTAAAACCAATTCGTTTATAAAGTGTAGACAAAAAAAAAAAAAAAAAAAAAAAAAGAGTAAAATCATGAATTTAAACCTACAAAATGTCAAAAGAATCTAGATTTAATTGTGTCAATATTAATCTATTATACATTAAAAATAAATAAATAAAGACATCATCAAATCTTGTTAAACACGAAATTGGAAGTAATAAAACCTTTAGATCTTATTTTTGTCCTAAACTTTTAAGTTTGTTGTATTCTAGTCTCTAAAGTTTCAAAATGTCTATTTTGGTTCTTGAACTAAAAGAATCATTTTGGTTCTTACCGTTATTATGTTTTTAATATATTAATTACATAGTTTCAGCATATATATTAGCATATTAATATCAGCATCATTTCAAGTCACATAAGTTGGAGAGGAGCATCAAACAAATGATTATAAAATAATATAACAAGGATTAGAATTGTTATTTTAAAAATGTTTATGGACTAAGATAGATATTTTGAAGGTTTTAACAACCAAAATGAAAAAATTTTAAAAATTCAAGGACTAAAATAAATATTTTGAAAGTTTATGAGTCAAAAAAAAAAAAAATGTAAGTTTAGAACAAAATACTATCTAAAAAAACCAAAATACTATTTAAACTTAGAGCTTTTAAAATTAGAACAAAACTTGGCTGCATACAGGTATGTTAGGGGGACTTGGCTGCATACAAGTACGTAGGGATCTCCCTACATGCCTCATTAAAATCTTGACAAGTAAGAAAGAGGAAGGGAGAAAATGAGAAAAAATTTTATTGTCAACATTTTAATGGTGGTATGCAGGGAGATCCCTATATAGCAGTATGCAGTCAAGTTTTTCACTTAAAATTATCTGTGTTTAAAATTTAGGGATGTAGTTTATATCAGGGAGGACTAGATTTGTAATTTTACCGATTAATTAACATAAATTCCCCTTGGGAAATTTCACTCATGCACGAAAAAACTTTGATCAATAAGCTAATATTTTGGGGATCTGGCAGAATGTGGCGCCAATTCCAGGGCCGGGCGACGTCGGAGCGCAGGCGGTGGCGATCAGAATCGGCGGGGACCAGGCGGCGTTTTGGAGCTGCGGATTCTTCGGCGCCCAAGACACTCTCCACGACGATCGCGGCCGCCATTACTTCAAGGATTGCTACATTCAAGGCTCCATCGATTTCATCTTCGGCAACGGAAGATCCTTCTACGAGGTAAGTCGGATTCGGAACCCGAATTTTATCTCCGATTTCGATTTGACGGTGAATTTCGGGATGAATTCGAAGATTTTATCGCATCGCAGGGTTGCCAGTTGATTTCGATGGCGAATCCGGTGCCTCAAGGATCGAAGTTCGTGAACGGAGCAGTGACGGCGCACGGCAGAGCCTCCGCCGAGGAGAACTCTGGATTTGCGTTTGTTAATTGCAGCATCGGTGGGACCGGCAGAGTGTGGCTCGGCCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTCGCCAACACCGTCATGACGGATATTATTGCGTCGGAGGGGTGGAATGACTTTAATGACCCTGCCAGAGACCAGTACGTTTTCTCAATTTTAAACTTGAAATACAAAATATATTGGTTAAAATATTCAATTTTAGTCTCCGAAAGTTTAGAATTAATTTTTATCTAAATTGGTTAAATAATAATAATAGTTTGGGAAAAATACACTTTTGGTCGGAGGTTTGGGTTAGGTTTCATTGTGGTCCCTGAGTTTTCAAACTCGACATTTTAGTGTATGAGTTTTGAATTTTGTTACATTCTTGGTCAATTTTGCCGTTAAACACTAAAAATACACTTTTGGTCACTTTTGGAAAAATACACTTTTGGTCAATTTTGCCGTTAAACACTAAAAATGTATTTTTCCCTAATAGTTTTCGTAAAAAGACATAATATAACATATTTCTAAAATTTATAGAGGTTAATTAATTTAGATAAATTAACAATAAATTTAGGGTAGGGATCCATTTTAGGTTTTAAAAAATATGGACTAAATTGTTTAGGTCCAGTTTAATAATCAATTGATTTTTGGTTTTTGAAAATTAAAATGATGCAATTTCCATCAATTTTTTTATTCTATATTTACTTTCTTCCTATGTTTTCAAAAGAACAAGTCAAATTTTGAAAACTAAAAAAAGTATTTTAAAAAACTTGTGTTTTGTTTCTAGAATTTGATTAGGAATTCAAATATGTTTTTGACAAAGATGAAAACATAAGAAAATTGGTGAAAACAAACATAAATTTCAAAAACTGAAAACTAAAAACCAAAAGGTTATCAAACAAACCTTAACGTTTATGGAATCACTTTATACAATGGAAAAAAGTTGCACAAAATTGTGATTAAAATCATTTTTAAGAAGATGTTACTAAAAACACAAGATTTTAAAAGTTAAAATCACTCCAACAATAAGAGTTTTGTTTTTTAAAGGTTAAATTATTAAAAATAGTCTTGAACTATGAGGTTGATTTCAATTATACCATTGAACTTCAAAATTTTCATTTTAGCTCCTAAAGTTTGAAATTTGTTTCAAAATAATCCTTGAAAGCTTTTTTTTTTTCATTAAATTTGTGGACGAAAAATGATGTGTTTGCATATATATTGTTGCTTATGTGAACAAATTTGAACACATACGTGGAAAAAACAATAAATATACATGCAAATATTTCACTTCTCATCCTTAAAATTAACGGAAAATATCTCTTCTTCTTAAAAATAAAAAAAAAAAATAGAAAATGTCTCCAAAGGTCGTTTTGAAACAAACTTTAAACTTCAAGGGTTAAATTGAAACTTTTGAAAGTTCAATGTTATAATTGAAACCAATCTTATAGTTCAATGGTATTTTTTTTAATTAAAAAATGATTAAACCAATTCAAGCCGAACTCATCCAAAATCAAACATATAATTGATAAGACCACAAGAGAATTGATTTGATGCAGTGGTTTAAGGCGATGTTTGGGAGTGATTTTGGCATAGCCAAAATCAATTTTACCAATGTCAAAATCAATTTCATCTAAAATAAACATGTTTGGCAAAAAAATAAATTGATTTTGAAAAAATCTAAAATCAATTTAAATTGATTTTTAAAGAATTAGCTGGGAGGTGATTTTGGATTTGGTGTTGGACCGGGAATCACCTCCCAGATTTTACAATTTATTTGGGAGTCATAATGGTAAGAAGCCGCTGCTGGGTGGCGCCAACAACTTTTTTTTTTTTTTTTCTTTGAAAAACAACCTATTGAATTAACTTCACTAATGCCATGTCAAACTCTCTTTCTCATTCTCATGTCCCAAAAACCGCAATCTATTATTTTTTTTCTTTTCGGTTCTTATTTTTCTTATTAATTTTTCTTTACTTGAAACATTTTAGTTGGATGACATTGGAAGACAAAAAGGTTCGAATGTCTAGCTTCTTAGTTGAAATATATATCAATTATTGTTGAATTATTAGTGTTATTATGTTCACTTAACACCCAATTTTGACCTCTTAGTCAAAAGTATATATCATTTGAATCTCTGACTTCATGGTCGAGAGTACATGTCAATTATCATTGAACTAAGCTTACTTTGGCATAATAGAACAATACTCGACTGCATAACAAGACATAGGGATTTTTCTACCATAGAACATTTCAAGATTAGATGAGATATGACTTTTGAATTTTTATTTTGATATGTATTATATGTTTTTCTTACCTAACATAATTTATAAAAGTTTAACAATAAAAGAAATGAAAAAATAATTAATAAAAATTGATATTTATCATAATTATTTAGTTAAATAATTTATTGAATGTAAAATGTCATTTTTAGTAATTTGGTAATTCTAAATCACTTTTGAAACCAAACATAATATTTCAAAATCACTTTTAAAAATTTGGAACCAAACATAAATATGGTTGCATGAAAATCAATTTTACAAAATCTCTTTTCCCAAAATCAATAATTCAATCATCACTCCCAAACATAGTCTAAGTGCATGTTTGGAAGTGATTTTGAAATGACTGAGATCATTTTTGTCAGAAATCATCCGTAAACATAGCTTTAATTATTCAAAATCAATTTCATGTTTTATTTCACACTTTTGAACTTGATTTTCATAACATCAATTTTGATCGGAAAAGTTGTTTTAACCATTTTAAAATCATTTCCAAACATACAAGTAATGAAAATTGAAAAAGATTGGGATTGAATGATTGCAGGACAGTGTTCTATGGAGAGTACAACTGCAGTGGAGCAGGAGCCAATCTGACTGCAAGGGCTCCATTTGTTCAGAGGCTCAATGATACACTAGCTTCCCCTTTCCTCAACTTATCCTTCATTGATGCTGACCAATGGCTCCAACCCTATTAATTTTTTTTTTTTTTTTTTAATTTTGTATATACATATATTCTTTTCATTCTCATTACATT

mRNA sequence

ATGAAGATTATCAATCATAATCTCCTCACAAACATTGCTCTCTCTTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCCCGCCACTCGCTTTCTCTCCGACCTCGAGTTTTCCTTCCTCCGCCACCTCGTCGACTGCCATCATCATCACCACCACCACCGTCACCACTACCATCACCATCATCACCATCACAAAAGTCCGACGGACGAAGAAGACCAAGCAACCATCAGTTCCATTTGCGACACGTTCCCAACCAACCTTCTCCCACCAGAAACCACCAACACTTCTGTTTTCTGCGTCGATCGGAACGGTTGTTGCAACTTCACGACGGTTCAGGCTGCAGTCGACTCCGTACCGAACCTGAGCCCGAAACGGAGCGTGATATGGATCAACAGTGGAATCTACTATGAGAAGGTGATGGTTCCAAGATCAAAACCGAACGTGACATTTCAAGGACAAGGCTACACGACCACGGCCATTGTTTGGAACGACACTGCCAACTCTTCGCATGGGACTTTTTACAGTGGCTCTGTTCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGAATGTGGCGCCAATTCCAGGGCCGGGCGACGTCGGAGCGCAGGCGGTGGCGATCAGAATCGGCGGGGACCAGGCGGCGTTTTGGAGCTGCGGATTCTTCGGCGCCCAAGACACTCTCCACGACGATCGCGGCCGCCATTACTTCAAGGATTGCTACATTCAAGGCTCCATCGATTTCATCTTCGGCAACGGAAGATCCTTCTACGAGGGTTGCCAGTTGATTTCGATGGCGAATCCGGTGCCTCAAGGATCGAAGTTCGTGAACGGAGCAGTGACGGCGCACGGCAGAGCCTCCGCCGAGGAGAACTCTGGATTTGCGTTTGTTAATTGCAGCATCGGTGGGACCGGCAGAGTGTGGCTCGGCCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTCGCCAACACCGTCATGACGGATATTATTGCGTCGGAGGGGTGGAATGACTTTAATGACCCTGCCAGAGACCAGACAGTGTTCTATGGAGAGTACAACTGCAGTGGAGCAGGAGCCAATCTGACTGCAAGGGCTCCATTTGTTCAGAGGCTCAATGATACACTAGCTTCCCCTTTCCTCAACTTATCCTTCATTGATGCTGACCAATGGCTCCAACCCTATTAA

Coding sequence (CDS)

ATGAAGATTATCAATCATAATCTCCTCACAAACATTGCTCTCTCTTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCCCGCCACTCGCTTTCTCTCCGACCTCGAGTTTTCCTTCCTCCGCCACCTCGTCGACTGCCATCATCATCACCACCACCACCGTCACCACTACCATCACCATCATCACCATCACAAAAGTCCGACGGACGAAGAAGACCAAGCAACCATCAGTTCCATTTGCGACACGTTCCCAACCAACCTTCTCCCACCAGAAACCACCAACACTTCTGTTTTCTGCGTCGATCGGAACGGTTGTTGCAACTTCACGACGGTTCAGGCTGCAGTCGACTCCGTACCGAACCTGAGCCCGAAACGGAGCGTGATATGGATCAACAGTGGAATCTACTATGAGAAGGTGATGGTTCCAAGATCAAAACCGAACGTGACATTTCAAGGACAAGGCTACACGACCACGGCCATTGTTTGGAACGACACTGCCAACTCTTCGCATGGGACTTTTTACAGTGGCTCTGTTCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGAATGTGGCGCCAATTCCAGGGCCGGGCGACGTCGGAGCGCAGGCGGTGGCGATCAGAATCGGCGGGGACCAGGCGGCGTTTTGGAGCTGCGGATTCTTCGGCGCCCAAGACACTCTCCACGACGATCGCGGCCGCCATTACTTCAAGGATTGCTACATTCAAGGCTCCATCGATTTCATCTTCGGCAACGGAAGATCCTTCTACGAGGGTTGCCAGTTGATTTCGATGGCGAATCCGGTGCCTCAAGGATCGAAGTTCGTGAACGGAGCAGTGACGGCGCACGGCAGAGCCTCCGCCGAGGAGAACTCTGGATTTGCGTTTGTTAATTGCAGCATCGGTGGGACCGGCAGAGTGTGGCTCGGCCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTCGCCAACACCGTCATGACGGATATTATTGCGTCGGAGGGGTGGAATGACTTTAATGACCCTGCCAGAGACCAGACAGTGTTCTATGGAGAGTACAACTGCAGTGGAGCAGGAGCCAATCTGACTGCAAGGGCTCCATTTGTTCAGAGGCTCAATGATACACTAGCTTCCCCTTTCCTCAACTTATCCTTCATTGATGCTGACCAATGGCTCCAACCCTATTAA

Protein sequence

MKIINHNLLTNIALSSLIPLLAFLLINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHHHHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAGANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY

Homology

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BLAST of Spg004228 vs. NCBI nr
Match: XP_038903629.1 (probable pectinesterase 8 [Benincasa hispida])

HSP 1 Score: 694.5 bits (1791), Expect = 5.3e-196
Identity = 343/395 (86.84%), Postives = 364/395 (92.15%), Query Frame = 0


Query: 6   HNLLTNIALSSLIPLLAFL-LINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHHH 65
           +++LTN+ALSSL+PLLAFL +I P   FLS+L     R  + CHHHHHH  HH+HHH  H
Sbjct: 8   NHVLTNLALSSLLPLLAFLFMIKPTPPFLSNL--VIFRDSIGCHHHHHH--HHHHHHRRH 67

Query: 66  HKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLSP 125
           HK+  DEEDQA ISSICDTFP  LLPPET NTSVFCVDRNGCCNFTTVQAAVD+VP LS 
Sbjct: 68  HKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPILSF 127

Query: 126 KRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 185
           KR++IWIN GIYYEKV+VP+SK NVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS
Sbjct: 128 KRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 187

Query: 186 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 245
           NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD
Sbjct: 188 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 247

Query: 246 CYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVNC 305
           CYIQGSIDFIFGNGRSFYE CQLISMANPVPQGSKFVNGAVTAHGRASA+ENSGF FVNC
Sbjct: 248 CYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVFVNC 307

Query: 306 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAGA 365
           SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQT+FYGEYNC+GAGA
Sbjct: 308 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNGAGA 367

Query: 366 NLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           N+++RAPFVQRLNDT ASPFLNLSFIDADQWLQPY
Sbjct: 368 NMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 398

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BLAST of Spg004228 vs. NCBI nr
Match: XP_022136420.1 (probable pectinesterase 8 [Momordica charantia])

HSP 1 Score: 693.0 bits (1787), Expect = 1.6e-195
Identity = 345/396 (87.12%), Postives = 357/396 (90.15%), Query Frame = 0


Query: 5   NHNLLTNIALSSLIPLLAFLLIN-PATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHH 64
           N N L  IALSSLIPLLAFLLIN PA RFLSDL+F+FLR  V C  HHHH     HHHH 
Sbjct: 3   NLNNLMTIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHH-----HHHHR 62

Query: 65  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 124
            H+SP DEEDQA  SSICD FP +LLP ET  TSVFCVDRNGCCNFTTVQAAVD+VPNLS
Sbjct: 63  RHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLS 122

Query: 125 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 184
            KR++IWINSGIYYEKVMVPRSK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQVFS
Sbjct: 123 LKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFS 182

Query: 185 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 244
           SNFIAKNISFMNVAPIPGPGDVGAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFK
Sbjct: 183 SNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFK 242

Query: 245 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 304
           DCYIQGSIDFIFGNGRSFYE CQLISMANPVPQGSKFVNGAVTAHGRASA+ENSGFAFVN
Sbjct: 243 DCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVN 302

Query: 305 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 364
           CS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIA EGWNDFNDPARDQT+FYGEYNCSGAG
Sbjct: 303 CSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAG 362

Query: 365 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           AN  ARAPFVQRLNDT  SPFLNLSFIDADQWL PY
Sbjct: 363 ANTGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

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BLAST of Spg004228 vs. NCBI nr
Match: XP_008451885.1 (PREDICTED: probable pectinesterase 8 [Cucumis melo])

HSP 1 Score: 679.5 bits (1752), Expect = 1.8e-191
Identity = 334/395 (84.56%), Postives = 358/395 (90.63%), Query Frame = 0


Query: 6   HNLLTNIALSSLIPLLAFL-LINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHHH 65
           +N L N+ LSSL+PLLAFL +INP   +LS+L        VDC H          HHHHH
Sbjct: 9   NNFLINLTLSSLLPLLAFLFMINPTPSYLSNL--VVFGDPVDCPH---------PHHHHH 68

Query: 66  HKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLSP 125
           H+ P DE+DQ+ ISSICD FPTNL+PPETTNTSVFCVD+NGCCNFTTVQAAVD+VPNLS 
Sbjct: 69  HEDP-DEDDQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPNLSI 128

Query: 126 KRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 185
           KR++IWIN+GIYYEKVMVP+SKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS
Sbjct: 129 KRNIIWINAGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 188

Query: 186 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 245
           NFIAKNISFMNVAPIPGPGDVGAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRHYFKD
Sbjct: 189 NFIAKNISFMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 248

Query: 246 CYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVNC 305
           CYIQGSIDFIFGNGRSFYE CQL+SMANPVPQGSKFVNGAVTAHGRASAEENSGF+FVNC
Sbjct: 249 CYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNC 308

Query: 306 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAGA 365
           SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDP RDQT+FYGEYNCSGAGA
Sbjct: 309 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGA 368

Query: 366 NLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           N+++RAP+VQRLNDT  S FLNLSFIDADQWLQPY
Sbjct: 369 NMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of Spg004228 vs. NCBI nr
Match: KAG7015100.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.8 bits (1732), Expect = 3.7e-189
Identity = 333/396 (84.09%), Postives = 356/396 (89.90%), Query Frame = 0


Query: 6   HNLLTNIALSSLIPL-LAFLL-INPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHH 65
           +N+L NIALSSL+PL LA LL + P   FLS L+ S     + C             HHH
Sbjct: 4   NNVLINIALSSLVPLFLALLLVVIPTPPFLSVLDDS-----LSC-------------HHH 63

Query: 66  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 125
           HHKS  DEED+A+I SICDTFPT LLPPET NTSV CVD+NGCCNFTTVQAAVD+V N+S
Sbjct: 64  HHKSSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMS 123

Query: 126 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 185
            KRS+IWIN+GIY+EKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS
Sbjct: 124 SKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 183

Query: 186 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 245
           SNFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFK
Sbjct: 184 SNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFK 243

Query: 246 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 305
           DCYIQGSIDFIFGNGRS+YE CQ+ISMANPVPQGSKFVNGAVTAHGRASA+ENSGFAFVN
Sbjct: 244 DCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVN 303

Query: 306 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 365
           CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQT+FYGEYNCSGAG
Sbjct: 304 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAG 363

Query: 366 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           AN++ARAP+VQRLNDT ASPFLNLSFIDADQWLQPY
Sbjct: 364 ANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 381

[Close]

BLAST of Spg004228 vs. NCBI nr
Match: KAG6577098.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.4 bits (1731), Expect = 4.8e-189
Identity = 332/396 (83.84%), Postives = 356/396 (89.90%), Query Frame = 0


Query: 6   HNLLTNIALSSLIPL-LAFLL-INPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHH 65
           +N+L NIALSSL+PL LA LL + P   FLS L+ S     + C             HHH
Sbjct: 4   NNVLINIALSSLVPLFLALLLVVIPTPPFLSVLDDS-----LSC-------------HHH 63

Query: 66  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 125
           HHKS  DEED+A+I SICDTFPT LLPPET NTS+ CVD+NGCCNFTTVQAAVD+V N+S
Sbjct: 64  HHKSSADEEDRASIGSICDTFPTALLPPETVNTSILCVDQNGCCNFTTVQAAVDAVLNMS 123

Query: 126 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 185
            KRS+IWIN+GIY+EKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS
Sbjct: 124 SKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 183

Query: 186 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 245
           SNFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFK
Sbjct: 184 SNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFK 243

Query: 246 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 305
           DCYIQGSIDFIFGNGRS+YE CQ+ISMANPVPQGSKFVNGAVTAHGRASA+ENSGFAFVN
Sbjct: 244 DCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVN 303

Query: 306 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 365
           CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQT+FYGEYNCSGAG
Sbjct: 304 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAG 363

Query: 366 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           AN++ARAP+VQRLNDT ASPFLNLSFIDADQWLQPY
Sbjct: 364 ANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 381

[Close]

BLAST of Spg004228 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 530.4 bits (1365), Expect = 1.7e-149
Identity = 257/397 (64.74%), Postives = 316/397 (79.60%), Query Frame = 0


Query: 1   MKIINHNLLTNIALSSLIPLLAFLLINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHH 60
           MKII+ ++   IA+ +++        +P    +  + +SF + L D HHHHHHH    HH
Sbjct: 1   MKIISLSISIGIAIIAVLASKTLFKTHPEAFGIKAISYSFKKSLCDHHHHHHHH----HH 60

Query: 61  HHHHHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVP 120
           HHH HK P+D + +    SICD FP N+ P +T  TS  CVD+NGCCNFTTVQ+AVD+V 
Sbjct: 61  HHHRHK-PSDTKRKV---SICDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVG 120

Query: 121 NLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 180
           N S +R+VIWINSG+YYEKV++P++KPN+T QGQG+  TAI WNDTA S++GTFY  +VQ
Sbjct: 121 NFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQ 180

Query: 181 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH 240
           VF S F+AKNISFMNVAPIP PGDVGAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRH
Sbjct: 181 VFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRH 240

Query: 241 YFKDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFA 300
           YFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S +ENSGF+
Sbjct: 241 YFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFS 300

Query: 301 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCS 360
           FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IA EGWN+FNDP+RD T+FYGEYNCS
Sbjct: 301 FVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCS 360

Query: 361 GAGANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQ 398
           G GA+++ RAP+VQ+LN+T  +  +N SFID DQWLQ
Sbjct: 361 GPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

[Close]

BLAST of Spg004228 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.5e-95
Identity = 193/394 (48.98%), Postives = 260/394 (65.99%), Query Frame = 0


Query: 8   LLTNIALSSLIPLLAFLLINPATRFLSDLEFSF----LRHLVDCHHHHHHHRHHYHHHHH 67
           L T IAL S+I L  + +  P++R LS +  +            H HHHHH HH+HHHH+
Sbjct: 7   LTTLIALLSII-LALYTIQLPSSRSLSSIATTIGALAKYSTFFGHKHHHHHHHHHHHHHY 66

Query: 68  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 127
           HH  P            C+ + T+ L  +   + V  VD +GC NF+ VQ+A+D VP+LS
Sbjct: 67  HHHEPI---------KCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLS 126

Query: 128 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 187
             +++I +NSG Y EKV V  +K N+  QG+GY  T+I WNDTA S+  T  S S  VF+
Sbjct: 127 SSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFA 186

Query: 188 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 247
           +NF A NISF N AP P PG+  AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH+FK
Sbjct: 187 ANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFK 246

Query: 248 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 307
           +C+IQGSIDFIFGNGRS Y+ C + S+A     G   V G++TA GR S +E SGF+FVN
Sbjct: 247 ECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVN 306

Query: 308 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 367
           C I G+G + LGRAW  ++ VVF+NT M+ II  EGWN++ D  +++TV +GE+ C G G
Sbjct: 307 CKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPG 366

Query: 368 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQ 398
           A+   R  F ++L D+ AS F+++SFID D+WL+
Sbjct: 367 ADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of Spg004228 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 9.4e-87
Identity = 163/334 (48.80%), Postives = 217/334 (64.97%), Query Frame = 0


Query: 67  SPTDEEDQAT-ISSICD---TFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNL 126
           SP +  DQ     S CD    FPT         T V  V  NGC  F  VQ A+D+    
Sbjct: 12  SPIESVDQRIHHPSKCDHLSKFPTKGF------TMVLKVSLNGCGRFKRVQDAIDASIGS 71

Query: 127 SPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVF 186
           S  +++I I+ GIY E+ +V  +K N+  QG GY+ T+I WN+T  SS+GTF S SV VF
Sbjct: 72  SQSKTLILIDFGIYRERFIVHENKNNLVVQGMGYSRTSIEWNNTTASSNGTFSSFSVAVF 131

Query: 187 SSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYF 246
              F A NISF N AP P PG V AQAVA+++ GD+AAF+ CGF+G QDTL D  GRH+F
Sbjct: 132 GEKFTAYNISFKNTAPAPNPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFF 191

Query: 247 KDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFV 306
           K C+I+GSIDFIFGNGRS YE C L S+A       +   G +TA+G+ + ++ +GF FV
Sbjct: 192 KGCFIEGSIDFIFGNGRSLYEDCTLHSIA------KENTIGCITANGKDTLKDRTGFVFV 251

Query: 307 NCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGA 366
           NC I G+ RVWLGRAWRP++RV+F+ T M+ +++ +GWND  DP   +TV+YGE+ C G 
Sbjct: 252 NCKITGSARVWLGRAWRPYARVIFSKTYMSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGP 311

Query: 367 GANLTARAPFVQRLNDTLASPFLNLSFIDADQWL 397
           GAN + R  + + L+D  A+PF N+SFID ++WL
Sbjct: 312 GANHSKRVTYAKLLSDVEAAPFTNISFIDGEEWL 333

[Close]

BLAST of Spg004228 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.0e-81
Identity = 147/296 (49.66%), Postives = 200/296 (67.57%), Query Frame = 0


Query: 108 NFTTVQAAVDSVPNLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTA 167
           +FT +Q A+DS+P ++  R VI +++G+Y EKV +P  K  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 168 NSSH------GTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAF 227
            +        GT+ S S  V S  F+AKNI+F N  P+P PG VG QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 228 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFV 287
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YEGC + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 288 NGAVTAHGRASAEENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWN 347
            GAVTA GR+S  E++GF+FV C + GTG ++LGRAW PFSRVVFA T M +II   GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 348 DFNDPARDQTVFYGEYNCSGAGANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQ 398
           ++ DP+R+ TVFYG+Y C+GAGAN   R  + + L D  A PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of Spg004228 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-73
Identity = 147/308 (47.73%), Postives = 198/308 (64.29%), Query Frame = 0


Query: 98  VFCVDRNGCCNFTTVQAAVDSVPNLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYT 157
           V  V  NG   F +VQ AVDS+P  + K   I I  G Y EKV+VP +KP +TF+G G  
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 158 TTAIVWNDTAN--SSHG----TFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA 217
            TAI W+D A+   ++G    T+ + SV V+++ F A+NISF N AP P PG  G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 218 IRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYEGCQLISMA 277
            RI GD+A F  CGF+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C+L S+A
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 278 NPVPQGSKFVNGAVTAHGRASAEENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVM 337
                 S+F  G++ AHGR   EE +GFAFV C + GTG +++GRA   +SR+V+A T  
Sbjct: 240 ------SRF--GSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYF 299

Query: 338 TDIIASEGWNDFNDPA-RDQTVFYGEYNCSGAGANLTARAPFVQRLNDTLASPFLNLSFI 397
             ++A  GW+D++  + + +T F+G YNC G GA  T    + + L+   A PF+  SF+
Sbjct: 300 DALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFV 359

Query: 398 DADQWLQP 399
           +   W+ P
Sbjct: 360 NGRHWIAP 359

[Close]

BLAST of Spg004228 vs. ExPASy TrEMBL
Match: A0A6J1C3U9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.5e-196
Identity = 345/396 (87.12%), Postives = 357/396 (90.15%), Query Frame = 0


Query: 5   NHNLLTNIALSSLIPLLAFLLIN-PATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHH 64
           N N L  IALSSLIPLLAFLLIN PA RFLSDL+F+FLR  V C  HHHH     HHHH 
Sbjct: 3   NLNNLMTIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHH-----HHHHR 62

Query: 65  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 124
            H+SP DEEDQA  SSICD FP +LLP ET  TSVFCVDRNGCCNFTTVQAAVD+VPNLS
Sbjct: 63  RHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLS 122

Query: 125 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 184
            KR++IWINSGIYYEKVMVPRSK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQVFS
Sbjct: 123 LKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFS 182

Query: 185 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 244
           SNFIAKNISFMNVAPIPGPGDVGAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFK
Sbjct: 183 SNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFK 242

Query: 245 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 304
           DCYIQGSIDFIFGNGRSFYE CQLISMANPVPQGSKFVNGAVTAHGRASA+ENSGFAFVN
Sbjct: 243 DCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVN 302

Query: 305 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 364
           CS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIA EGWNDFNDPARDQT+FYGEYNCSGAG
Sbjct: 303 CSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAG 362

Query: 365 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           AN  ARAPFVQRLNDT  SPFLNLSFIDADQWL PY
Sbjct: 363 ANTGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

[Close]

BLAST of Spg004228 vs. ExPASy TrEMBL
Match: A0A1S3BRY7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.6e-192
Identity = 334/395 (84.56%), Postives = 358/395 (90.63%), Query Frame = 0


Query: 6   HNLLTNIALSSLIPLLAFL-LINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHHH 65
           +N L N+ LSSL+PLLAFL +INP   +LS+L        VDC H          HHHHH
Sbjct: 9   NNFLINLTLSSLLPLLAFLFMINPTPSYLSNL--VVFGDPVDCPH---------PHHHHH 68

Query: 66  HKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLSP 125
           H+ P DE+DQ+ ISSICD FPTNL+PPETTNTSVFCVD+NGCCNFTTVQAAVD+VPNLS 
Sbjct: 69  HEDP-DEDDQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPNLSI 128

Query: 126 KRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 185
           KR++IWIN+GIYYEKVMVP+SKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS
Sbjct: 129 KRNIIWINAGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 188

Query: 186 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 245
           NFIAKNISFMNVAPIPGPGDVGAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRHYFKD
Sbjct: 189 NFIAKNISFMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 248

Query: 246 CYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVNC 305
           CYIQGSIDFIFGNGRSFYE CQL+SMANPVPQGSKFVNGAVTAHGRASAEENSGF+FVNC
Sbjct: 249 CYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNC 308

Query: 306 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAGA 365
           SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDP RDQT+FYGEYNCSGAGA
Sbjct: 309 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGA 368

Query: 366 NLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           N+++RAP+VQRLNDT  S FLNLSFIDADQWLQPY
Sbjct: 369 NMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of Spg004228 vs. ExPASy TrEMBL
Match: A0A6J1ETE2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.7e-187
Identity = 330/397 (83.12%), Postives = 352/397 (88.66%), Query Frame = 0


Query: 5   NHNLLTNIALSSLIPL-LAFLL-INPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHH 64
           ++NLL NIALSSL+PL LA LL + P   F     FS     + C             HH
Sbjct: 3   HNNLLINIALSSLVPLFLALLLVVIPTPPF-----FSVPDDSLSC-------------HH 62

Query: 65  HHHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNL 124
           HHHK   DEED+A+I SICDTFPT LLPPET NTSV CVD+NGCCNFTTVQAAVD+V N+
Sbjct: 63  HHHKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNM 122

Query: 125 SPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVF 184
           S KRS+IWIN+GIY+EKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVF
Sbjct: 123 SSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVF 182

Query: 185 SSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYF 244
           SSNFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYF
Sbjct: 183 SSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYF 242

Query: 245 KDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFV 304
           KDCYIQGSIDFIFGNGRS+YE CQ+ISMANPVPQGSKFVNGAVTAHGRASA+ENSGFAFV
Sbjct: 243 KDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFV 302

Query: 305 NCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGA 364
           NCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQT+FYGEYNCSGA
Sbjct: 303 NCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGA 362

Query: 365 GANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           GAN++ARAP+VQRLNDT  SPFLNLSFIDADQWLQPY
Sbjct: 363 GANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQPY 381

[Close]

BLAST of Spg004228 vs. ExPASy TrEMBL
Match: A0A0A0KVA9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.9e-186
Identity = 328/399 (82.21%), Postives = 357/399 (89.47%), Query Frame = 0


Query: 3   IINHN-LLTNIALSSLIPLLAFL-LINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHH 62
           I+N+N  L N+ LSSL+PLLAFL +INP   FLS+L        VDC HH        HH
Sbjct: 4   IMNYNHFLINLTLSSLLPLLAFLFMINPTPSFLSNL--VVFGDPVDCPHH--------HH 63

Query: 63  HHHHHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVP 122
            HHHH++P DE+DQ+ ISSICD FPT L+P ETTNTS+FCVD+N CCNFTT+QAAVD+VP
Sbjct: 64  PHHHHENP-DEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVP 123

Query: 123 NLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 182
           NLS KR++IWIN+GIYYEKVMVP++K NVTFQGQGYTTTAIVWNDTANSSHGTFYS SVQ
Sbjct: 124 NLSIKRNIIWINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQ 183

Query: 183 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH 242
           VFSSNFIAKN+SFMNVAPIP PGDVGAQ VAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH
Sbjct: 184 VFSSNFIAKNLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH 243

Query: 243 YFKDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFA 302
           YFKDCYIQGSIDFIFGNGRSFYE CQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+
Sbjct: 244 YFKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFS 303

Query: 303 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCS 362
           FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQT+FYGEYNCS
Sbjct: 304 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCS 363

Query: 363 GAGANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           GAGAN+++RAP+VQRLNDT  S FLNLSFIDADQWLQPY
Sbjct: 364 GAGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of Spg004228 vs. ExPASy TrEMBL
Match: A0A6J1J3X1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.4e-186
Identity = 329/396 (83.08%), Postives = 352/396 (88.89%), Query Frame = 0


Query: 6   HNLLTNIALSSLIPL-LAFLL-INPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHHHHH 65
           +N+L NIALSSL+PL LA LL + P   FLS L+ S     ++C            HHHH
Sbjct: 4   NNVLINIALSSLVPLFLALLLVVIPTPPFLSVLDDS-----LNC------------HHHH 63

Query: 66  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 125
           HHK   DEEDQ +I SICDTFPT LLP ET +TSV CVD NGCCNFTTVQAAVD+V N+S
Sbjct: 64  HHKPSADEEDQTSIGSICDTFPTALLPLETVDTSVLCVDLNGCCNFTTVQAAVDAVLNMS 123

Query: 126 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 185
            KRS+IWIN+GIY+EKVMVPRSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFS
Sbjct: 124 SKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYKTTAIVWNDTANSSHGTFYSGSVQVFS 183

Query: 186 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 245
           SNFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFK
Sbjct: 184 SNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFK 243

Query: 246 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 305
           DCYIQGSIDFIFGNGRS+YE CQ+ISMANPVPQGSKFVNGAVTAHGRASA+ENSGFAFVN
Sbjct: 244 DCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVN 303

Query: 306 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 365
           CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQT+FYGEYNCSG G
Sbjct: 304 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGTG 363

Query: 366 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQPY 400
           AN++ARAP+VQRLNDT ASPFLNLSFIDADQWLQPY
Sbjct: 364 ANMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 382

[Close]

BLAST of Spg004228 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.2e-150
Identity = 257/397 (64.74%), Postives = 316/397 (79.60%), Query Frame = 0


Query: 1   MKIINHNLLTNIALSSLIPLLAFLLINPATRFLSDLEFSFLRHLVDCHHHHHHHRHHYHH 60
           MKII+ ++   IA+ +++        +P    +  + +SF + L D HHHHHHH    HH
Sbjct: 1   MKIISLSISIGIAIIAVLASKTLFKTHPEAFGIKAISYSFKKSLCDHHHHHHHH----HH 60

Query: 61  HHHHHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVP 120
           HHH HK P+D + +    SICD FP N+ P +T  TS  CVD+NGCCNFTTVQ+AVD+V 
Sbjct: 61  HHHRHK-PSDTKRKV---SICDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVG 120

Query: 121 NLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 180
           N S +R+VIWINSG+YYEKV++P++KPN+T QGQG+  TAI WNDTA S++GTFY  +VQ
Sbjct: 121 NFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQ 180

Query: 181 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH 240
           VF S F+AKNISFMNVAPIP PGDVGAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRH
Sbjct: 181 VFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRH 240

Query: 241 YFKDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFA 300
           YFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S +ENSGF+
Sbjct: 241 YFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFS 300

Query: 301 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCS 360
           FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IA EGWN+FNDP+RD T+FYGEYNCS
Sbjct: 301 FVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCS 360

Query: 361 GAGANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQ 398
           G GA+++ RAP+VQ+LN+T  +  +N SFID DQWLQ
Sbjct: 361 GPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

[Close]

BLAST of Spg004228 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.9e-96
Identity = 193/394 (48.98%), Postives = 260/394 (65.99%), Query Frame = 0


Query: 8   LLTNIALSSLIPLLAFLLINPATRFLSDLEFSF----LRHLVDCHHHHHHHRHHYHHHHH 67
           L T IAL S+I L  + +  P++R LS +  +            H HHHHH HH+HHHH+
Sbjct: 7   LTTLIALLSII-LALYTIQLPSSRSLSSIATTIGALAKYSTFFGHKHHHHHHHHHHHHHY 66

Query: 68  HHKSPTDEEDQATISSICDTFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNLS 127
           HH  P            C+ + T+ L  +   + V  VD +GC NF+ VQ+A+D VP+LS
Sbjct: 67  HHHEPI---------KCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLS 126

Query: 128 PKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFS 187
             +++I +NSG Y EKV V  +K N+  QG+GY  T+I WNDTA S+  T  S S  VF+
Sbjct: 127 SSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFA 186

Query: 188 SNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFK 247
           +NF A NISF N AP P PG+  AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH+FK
Sbjct: 187 ANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFK 246

Query: 248 DCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFVN 307
           +C+IQGSIDFIFGNGRS Y+ C + S+A     G   V G++TA GR S +E SGF+FVN
Sbjct: 247 ECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVN 306

Query: 308 CSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGAG 367
           C I G+G + LGRAW  ++ VVF+NT M+ II  EGWN++ D  +++TV +GE+ C G G
Sbjct: 307 CKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPG 366

Query: 368 ANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQ 398
           A+   R  F ++L D+ AS F+++SFID D+WL+
Sbjct: 367 ADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of Spg004228 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 6.7e-88
Identity = 163/334 (48.80%), Postives = 217/334 (64.97%), Query Frame = 0


Query: 67  SPTDEEDQAT-ISSICD---TFPTNLLPPETTNTSVFCVDRNGCCNFTTVQAAVDSVPNL 126
           SP +  DQ     S CD    FPT         T V  V  NGC  F  VQ A+D+    
Sbjct: 12  SPIESVDQRIHHPSKCDHLSKFPTKGF------TMVLKVSLNGCGRFKRVQDAIDASIGS 71

Query: 127 SPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVF 186
           S  +++I I+ GIY E+ +V  +K N+  QG GY+ T+I WN+T  SS+GTF S SV VF
Sbjct: 72  SQSKTLILIDFGIYRERFIVHENKNNLVVQGMGYSRTSIEWNNTTASSNGTFSSFSVAVF 131

Query: 187 SSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYF 246
              F A NISF N AP P PG V AQAVA+++ GD+AAF+ CGF+G QDTL D  GRH+F
Sbjct: 132 GEKFTAYNISFKNTAPAPNPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFF 191

Query: 247 KDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFVNGAVTAHGRASAEENSGFAFV 306
           K C+I+GSIDFIFGNGRS YE C L S+A       +   G +TA+G+ + ++ +GF FV
Sbjct: 192 KGCFIEGSIDFIFGNGRSLYEDCTLHSIA------KENTIGCITANGKDTLKDRTGFVFV 251

Query: 307 NCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTVFYGEYNCSGA 366
           NC I G+ RVWLGRAWRP++RV+F+ T M+ +++ +GWND  DP   +TV+YGE+ C G 
Sbjct: 252 NCKITGSARVWLGRAWRPYARVIFSKTYMSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGP 311

Query: 367 GANLTARAPFVQRLNDTLASPFLNLSFIDADQWL 397
           GAN + R  + + L+D  A+PF N+SFID ++WL
Sbjct: 312 GANHSKRVTYAKLLSDVEAAPFTNISFIDGEEWL 333

[Close]

BLAST of Spg004228 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.4e-82
Identity = 147/296 (49.66%), Postives = 200/296 (67.57%), Query Frame = 0


Query: 108 NFTTVQAAVDSVPNLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYTTTAIVWNDTA 167
           +FT +Q A+DS+P ++  R VI +++G+Y EKV +P  K  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 168 NSSH------GTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAF 227
            +        GT+ S S  V S  F+AKNI+F N  P+P PG VG QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 228 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYEGCQLISMANPVPQGSKFV 287
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YEGC + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 288 NGAVTAHGRASAEENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWN 347
            GAVTA GR+S  E++GF+FV C + GTG ++LGRAW PFSRVVFA T M +II   GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 348 DFNDPARDQTVFYGEYNCSGAGANLTARAPFVQRLNDTLASPFLNLSFIDADQWLQ 398
           ++ DP+R+ TVFYG+Y C+GAGAN   R  + + L D  A PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of Spg004228 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 8.5e-75
Identity = 147/308 (47.73%), Postives = 198/308 (64.29%), Query Frame = 0


Query: 98  VFCVDRNGCCNFTTVQAAVDSVPNLSPKRSVIWINSGIYYEKVMVPRSKPNVTFQGQGYT 157
           V  V  NG   F +VQ AVDS+P  + K   I I  G Y EKV+VP +KP +TF+G G  
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 158 TTAIVWNDTAN--SSHG----TFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA 217
            TAI W+D A+   ++G    T+ + SV V+++ F A+NISF N AP P PG  G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 218 IRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYEGCQLISMA 277
            RI GD+A F  CGF+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C+L S+A
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 278 NPVPQGSKFVNGAVTAHGRASAEENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVM 337
                 S+F  G++ AHGR   EE +GFAFV C + GTG +++GRA   +SR+V+A T  
Sbjct: 240 ------SRF--GSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYF 299

Query: 338 TDIIASEGWNDFNDPA-RDQTVFYGEYNCSGAGANLTARAPFVQRLNDTLASPFLNLSFI 397
             ++A  GW+D++  + + +T F+G YNC G GA  T    + + L+   A PF+  SF+
Sbjct: 300 DALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFV 359

Query: 398 DADQWLQP 399
           +   W+ P
Sbjct: 360 NGRHWIAP 359

The following BLAST results are available for this feature:

BLAST of Spg004228 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903629.1	5.3e-196	86.84	probable pectinesterase 8 [Benincasa hispida]	[more]
XP_022136420.1	1.6e-195	87.12	probable pectinesterase 8 [Momordica charantia]	[more]
XP_008451885.1	1.8e-191	84.56	PREDICTED: probable pectinesterase 8 [Cucumis melo]	[more]
KAG7015100.1	3.7e-189	84.09	putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6577098.1	4.8e-189	83.84	putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg004228 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23038	1.7e-149	64.74	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9ZQA3	5.5e-95	48.98	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q9ZQA4	9.4e-87	48.80	Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1	[more]
Q8VYZ3	2.0e-81	49.66	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q8LPF3	1.2e-73	47.73	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]

BLAST of Spg004228 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C3U9	7.5e-196	87.12	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1	[more]
A0A1S3BRY7	8.6e-192	84.56	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1	[more]
A0A6J1ETE2	3.7e-187	83.12	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1	[more]
A0A0A0KVA9	1.9e-186	82.21	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1	[more]
A0A6J1J3X1	2.4e-186	83.08	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1	[more]

BLAST of Spg004228 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05310.1	1.2e-150	64.74	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	3.9e-96	48.98	Pectin lyase-like superfamily protein	[more]
AT2G36700.1	6.7e-88	48.80	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	1.4e-82	49.66	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	8.5e-75	47.73	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 97..399 e-value: 3.2E-99 score: 334.2
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 101..391 e-value: 8.9E-68 score: 228.2
None	No IPR available	PANTHER	PTHR31321:SF33	PECTINESTERASE 8-RELATED	coord: 21..399
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 21..399
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 247..256
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 97..398

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg004228.1	Spg004228.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity