Spg004112 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg004112 vs. NCBI nr
Match: KAG6571861.1 (Cysteine proteinase inhibitor 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 140.6 bits (353), Expect = 1.6e-29
Identity = 62/101 (61.39%), Postives = 78/101 (77.23%), Query Frame = 0

Query: 1   MSSAMVVNVGQYDPIHRVINGVKDSCPSGWTSIVDVEEPYVQEVAKFSVTEYDIKYKENL 60
           MSS+ +VNVGQYDP HRVIN +KD CPSGWT I D++E  V+E AK  V EY+++Y E L
Sbjct: 1   MSSSTIVNVGQYDPGHRVINDLKDCCPSGWTPIPDIDEGCVKEAAKHGVAEYNMEYNETL 60

Query: 61  KYTCIARGWYMEQKEGGMDYAFILEALDCLGCVGKYKATMA 102
           KY  I R WYME KEGG+DY F+LEA+DC+G V +YKA ++
Sbjct: 61  KYEGIVRAWYMELKEGGLDYGFVLEAMDCVGRVNRYKAVVS 101

BLAST of Spg004112 vs. NCBI nr
Match: KAG6588756.1 (hypothetical protein SDJN03_17321, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 137.9 bits (346), Expect = 1.0e-28
Identity = 72/119 (60.50%), Postives = 88/119 (73.95%), Query Frame = 0

Query: 100 MAAIV-AQNNLVVDGKIDD---CSRLWIPIPNVSEICVQEVARLAVKEYNLEHQENFKYK 159
           MAA+V A N+ +   KID    C   WIPIPNVSEICVQ VA+ AV++YNLEH+E+FKYK
Sbjct: 1   MAAVVHAPNDPINQLKIDGNLLCPSKWIPIPNVSEICVQNVAKFAVEDYNLEHKESFKYK 60

Query: 160 ITLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSEKKELGDKLRKLVSSKRIEKN 215
              CGWFME K E L FRLHVEV DCLGR F+ + +VSEKK   +K+RKL S+K I+K+
Sbjct: 61  NIKCGWFMELKGEDLAFRLHVEVKDCLGRVFEMDVVVSEKKVCAEKVRKLESAKLIKKH 119

BLAST of Spg004112 vs. NCBI nr
Match: XP_023522970.1 (uncharacterized protein LOC111787034 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 131.0 bits (328), Expect = 1.3e-26
Identity = 64/118 (54.24%), Postives = 84/118 (71.19%), Query Frame = 0

Query: 100 MAAIVAQNNLVVDGKIDD---CSRLWIPIPNVSEICVQEVARLAVKEYNLEHQENFKYKI 159
           + A +A N+ +   KID    C   WI IP+VSE+CVQ +A+ AV++YNL H+E+FKYK 
Sbjct: 3   VVAHIASNDPISQLKIDGCLICPSKWIQIPDVSEVCVQNIAKFAVQDYNLIHKESFKYKS 62

Query: 160 TLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSEKKELGDKLRKLVSSKRIEKN 215
             CGWFME K E L F LH+EV DCLGR F+ + +VSEKK  G+K+RKL S+K I+KN
Sbjct: 63  IKCGWFMELKGEDLAFHLHIEVKDCLGRIFELDVVVSEKKACGEKVRKLESAKLIKKN 120

BLAST of Spg004112 vs. NCBI nr
Match: KAG6588757.1 (hypothetical protein SDJN03_17322, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.4 bits (324), Expect = 3.7e-26
Identity = 66/118 (55.93%), Postives = 83/118 (70.34%), Query Frame = 0

Query: 100 MAAIVAQNNLVVDGKIDD---CSRLWIPIPNVSEICVQEVARLAVKEYNLEHQENFKYKI 159
           + A VA N+ +   KID    C   WI IP+VSE+CVQ VA+ AV++YNL H+E+FKYK 
Sbjct: 3   VVAQVALNDPISQLKIDGCLICPSKWIQIPDVSEVCVQNVAKFAVEDYNLVHKESFKYKS 62

Query: 160 TLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSEKKELGDKLRKLVSSKRIEKN 215
             CGWFME K E L +RLHVEV DCLGR F  + +VSEKK   +K+RKL S+K I+KN
Sbjct: 63  IKCGWFMELKGEDLAYRLHVEVKDCLGRIFVLDVVVSEKKTCAEKIRKLESAKLIKKN 120

BLAST of Spg004112 vs. NCBI nr
Match: KAG6588758.1 (hypothetical protein SDJN03_17323, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.4 bits (324), Expect = 3.7e-26
Identity = 63/118 (53.39%), Postives = 83/118 (70.34%), Query Frame = 0

Query: 100 MAAIVAQNNLVVDGKIDD---CSRLWIPIPNVSEICVQEVARLAVKEYNLEHQENFKYKI 159
           + A +A N+ +   KID    C   WI IP+VSE+CVQ +A+ AV++YNL H+E+FKYK 
Sbjct: 3   VVAQIASNDPISQLKIDGCLICPSKWIQIPDVSEVCVQNIAKFAVEDYNLVHKESFKYKS 62

Query: 160 TLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSEKKELGDKLRKLVSSKRIEKN 215
             CGWFME K E L +RLH+EV DCLGR F+   +VSEKK   +K+RKL S+K I+KN
Sbjct: 63  IKCGWFMELKGEDLAYRLHIEVKDCLGRIFELNVVVSEKKTCAEKIRKLESAKLIKKN 120

BLAST of Spg004112 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.4e-04
Identity = 26/83 (31.33%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 18  VINGVKDSCPSGWTSIVDVEEPYVQEVAKFSVTEYDIKYKENLKYTCIARGWYMEQKEGG 77
           V+ G K     GW  I ++    VQ+VA+F+V+E++ +  + L+Y  + RG+   Q   G
Sbjct: 20  VVGGRKRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGY--TQVVAG 79

Query: 78  MDYAFILEALDCLGCVGKYKATM 101
            +Y  ++ A D    VG Y+A +
Sbjct: 80  TNYRLVIAAKDG-AVVGNYEAVV 99

BLAST of Spg004112 vs. ExPASy TrEMBL
Match: A0A6J1EIQ8 (uncharacterized protein LOC111434517 OS=Cucurbita moschata OX=3662 GN=LOC111434517 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.4e-23
Identity = 67/184 (36.41%), Postives = 103/184 (55.98%), Query Frame = 0

Query: 30  WTSIVDVEEPYVQEVAKFSVTEYDIKYKENLKYTCIARGWYMEQKEGGMDYAFILEALDC 89
           W  I +++  +V E++KF+V E++IKYK+ L +  I  GWYME     + +   L+A+DC
Sbjct: 124 WIKIPNLQAVFVIEISKFAVHEFNIKYKDYLVFDSIYEGWYMEMGRDDLKFRLKLKAIDC 183

Query: 90  LGCVGKYKATMAA--IVAQNNLVVDGKIDDCSRL----WIPIPNVSEICVQEVARLAVKE 149
           L  V  Y+A +    I+ +  L+++      S L    WI IP+V E CVQ VA  AV E
Sbjct: 184 LKRVNSYEAVVFVKNILGKKILILES--FQLSSLVVEKWIQIPSVKEPCVQVVAEFAVDE 243

Query: 150 YNLEHQENFKYKITLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSEKKELGDKLR 208
           +N ++    K+     GW+ E     L +RLH++  D L R   +EF+V E K   +++R
Sbjct: 244 FNRKYGNILKFNSVYEGWYWELSPNSLKYRLHIKAIDFLERCLNYEFVVFEVKYQSERVR 303

BLAST of Spg004112 vs. ExPASy TrEMBL
Match: A0A0A0K3W6 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-17
Identity = 58/191 (30.37%), Postives = 99/191 (51.83%), Query Frame = 0

Query: 25  SCPS--GWTSIVDVEEPYVQEVAKFSVTEYDIKYKENLKYTCIARGWYMEQKEGGMDYAF 84
           SC S   W  I D   P V EVAK  V +++    ++L Y  +  GW+ E  +  + +  
Sbjct: 2   SCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHRL 61

Query: 85  ILEALDCLGCVGKYKATMAAIVAQNNLV------VDGKIDDCSRL--WIPIPNVSEICVQ 144
           I+E +   G V  Y+  +  +      V      + G +D+ + L  WI IP+V + CVQ
Sbjct: 62  IIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCVQ 121

Query: 145 EVARLAVKEYNLEHQENFKYKITLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSE 204
           + A+ AV E+N    +   Y  T+ GW+ E    +++F+LH++  +C+GR   +  +V E
Sbjct: 122 DCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVKE 181

Query: 205 KKELGDKLRKL 206
           +K L +K++KL
Sbjct: 182 EKPLTEKIKKL 192

BLAST of Spg004112 vs. ExPASy TrEMBL
Match: A0A6J1JKF7 (uncharacterized protein LOC111486626 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486626 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 3.4e-17
Identity = 65/199 (32.66%), Postives = 99/199 (49.75%), Query Frame = 0

Query: 30  WTSIVDVEEPYVQEVAKFSVTEYDIKYKENLKYTCIARGWYMEQKEGGMDYAFILEALDC 89
           W  I DV    VQEVAK +V ++++++  +LKY  I  GWY E  +  + Y   + A+D 
Sbjct: 17  WIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAIDF 76

Query: 90  LGCVGKYKA--------------TMAAIVAQNNLVVDGKIDDCS-RLWIPIPNVSEICVQ 149
           L     Y+A               ++ +   N     G +D      WI IP++    VQ
Sbjct: 77  LQRSLLYEALVFEEKPKLERIRKLISFLYLLNGGHYVGPVDPPQVEKWIKIPDLRVPFVQ 136

Query: 150 EVARLAVKEYNLEHQENFKYKITLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSE 209
           EV++ A+ EYN +  E  KY     GW+ E   + + FRLHV+  DCLGR   +E IV  
Sbjct: 137 EVSKFAIDEYN-KGGEGLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEAIVLV 196

Query: 210 KKELGDKLRKLVSSKRIEK 214
           K+ L  K++ L S K +++
Sbjct: 197 KQFLSKKIKILESFKVLDQ 214

BLAST of Spg004112 vs. ExPASy TrEMBL
Match: A0A6J1JG70 (uncharacterized protein LOC111486626 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486626 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 3.4e-17
Identity = 65/199 (32.66%), Postives = 99/199 (49.75%), Query Frame = 0

Query: 30  WTSIVDVEEPYVQEVAKFSVTEYDIKYKENLKYTCIARGWYMEQKEGGMDYAFILEALDC 89
           W  I DV    VQEVAK +V ++++++  +LKY  I  GWY E  +  + Y   + A+D 
Sbjct: 17  WIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAIDF 76

Query: 90  LGCVGKYKA--------------TMAAIVAQNNLVVDGKIDDCS-RLWIPIPNVSEICVQ 149
           L     Y+A               ++ +   N     G +D      WI IP++    VQ
Sbjct: 77  LQRSLLYEALVFEEKPKLERIRKLISFLYLLNGGHYVGPVDPPQVEKWIKIPDLRVPFVQ 136

Query: 150 EVARLAVKEYNLEHQENFKYKITLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSE 209
           EV++ A+ EYN +  E  KY     GW+ E   + + FRLHV+  DCLGR   +E IV  
Sbjct: 137 EVSKFAIDEYN-KGGEGLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEAIVLV 196

Query: 210 KKELGDKLRKLVSSKRIEK 214
           K+ L  K++ L S K +++
Sbjct: 197 KQFLSKKIKILESFKVLDQ 214

BLAST of Spg004112 vs. ExPASy TrEMBL
Match: P94012 (Phloem filament protein OS=Cucurbita maxima OX=3661 GN=PP1 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.4e-17
Identity = 65/195 (33.33%), Postives = 96/195 (49.23%), Query Frame = 0

Query: 30  WTSIVDVEEPYVQEVAKFSVTEYDIKYKENLKYTCIARGWYMEQKEGGMDYAFILEALDC 89
           W  I DV    VQEVAK +V ++++++  +LKY  I  GWY E  +  + Y   + A+D 
Sbjct: 17  WIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAIDF 76

Query: 90  LGCVGKYKA--------------TMAAIVAQNNLVVDGKIDDCS-RLWIPIPNVSEICVQ 149
           L     Y+A               ++ +   N     G +D      WI IP++    VQ
Sbjct: 77  LQRSLLYEALVFEEKPKLERIRKLISFLYLLNGGHYVGPVDPPQVEKWIKIPDLRVPFVQ 136

Query: 150 EVARLAVKEYNLEHQENFKYKITLCGWFMEQKDEILLFRLHVEVTDCLGRTFKFEFIVSE 209
           EV++ A+ EYN +  E  KY     GW+ E   + + FRLHV+  DCLGR   +E IV  
Sbjct: 137 EVSKFAIDEYN-KGGEGLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEAIVLV 196